Fisetin

Catalog No.S2298

Fisetin Chemical Structure

Molecular Weight(MW): 286.24

Fisetin (Fustel) is a potent sirtuin activating compound (STAC) and an agent that modulates sirtuins.

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Cited by 2 Publications

1 Customer Review

  • Liver pathology and serum markers of liver injury in mice fed control (Ctrl), or ethanol (EtOH), or ethanol combined with fisetin (E/Fisetin) diet for 4 weeks. (A) Light microscopy with hematoxylin and eosin staining shows lipid accumulation (arrows) in the liver of mice after alcohol exposure, which was alleviated by fisetin administration. (B) Plasma alanine aminotransferase (ALT) and (C) aspartate aminotransferase (AST) activities. Infinity ALT and AST reagents were used formeasuring ALT and AST activities. Scale bar: 50 lm. Results are mean±SD (n = 3 to 6). Results for bars that do not share a letter differed significantly among groups (p < 0.05). Significant differences among groups are determined by analysis of variance followed by Tukey’s test.

    Alcohol Clin Exp Res, 2016, 40(10):2076-2084.. Fisetin purchased from Selleck.

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Biological Activity

Description Fisetin (Fustel) is a potent sirtuin activating compound (STAC) and an agent that modulates sirtuins.
Targets
Sirtuin [1]
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MDA-MB-453 Mkj6R4VtdCC4aXHibYxqfHliYYPzZZk> MXmwMEAzOCxiNECsJFYxNCB6MDygZY5lKDFyMDFCuW0> NVPy[lRWOjRuIES4MEBidmRiN{KgbI92enN? Mmj1W4l1cCCrbnPy[YF{cW6pIHPvcoNmdnS{YYTpc45{KG:oIH\pd4V1cW5iKECsJFIxNCB2MDygOlAtKDhyLDDhcoQhOTByINM1UUktKHSqZYLlJJdieyCjIHTlZ5Jm[XOnIH;id4VzfmWmIHnuJINmdGxiboXtZoVzKGmwIHTp[oZmemWwdDD0bY1mKGmwdHXyeoFteyBqMkSsJFQ5NCCjbnSgO|IhcG:3coOpMi=> MXyzNFQ{OTZ7Mh?=
SGC7901 MnvhVJJwdGmoZYLheIlwdiCjc4PhfS=> NYrVPFdYOCxiMTygOUwhOTBuIEG1JI9zKDJyINM1US=> M2\mfFQ5KGh? NX:5U3k5cW6qaXLpeJMheHKxbHnm[ZJifGmxbh?= MWiyPVgxPTV6MB?=
A549 NHnMOoNE\WyuII\pZYJqdGm2eTDhd5NigQ>? NH[1fIQxNCBzMDygNlAtKDNyIH;yJFQxKML3TR?= MnfyNlQtKDR6IH;yJFczKGh? NIjWUFBncXOndHnuJJdieyCnZn\lZ5RqfmViaX6gbY5pcWKrdHnu[{B1cGViZ4Lve5RpKG:oIFG1OFkh[2WubIOgbY4h[SCmb4PlMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy M33sZ|I6PDZ5OEW5
ARPE-19 M3vlcGNmdGxibXnndoF1cW:wIHHzd4F6 MYK1JI9zKDFyIN88US=> MlmyNlQhcA>? NFHYboxxemW2cnXheI1mdnRid3n0bEBncXOndHnuJI1iemunZHz5JJJm\HWlZXSgZ4VtdCCvaXfyZZRqd25iaX6gSWdHNXS{ZXH0[YQhSVKSRT2xPUBk\Wyucx?= MX[yPVI6PjB5MB?=
SCC-23 MYfD[YxtKH[rYXLpcIl1gSCjc4PhfS=> MWmwMVExOCEQvF2= MYSyOEBp MnXv[ol{\XSrbjDzbYdvcW[rY3HueIx6KGmwaHnibZRm\CClZXzsJJZq[WKrbHn0fUBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldkApe2mpbnnmbYNidnSueTD3bIVvKHSqZTDjc45k\W62cnH0bY9vKGm|IHjp[4hmeiC2aHHuJFIxKM7:TTm= M1XYPFI6OzF{NUKw
Tca-8113 MUXD[YxtKH[rYXLpcIl1gSCjc4PhfS=> NVjlZ3ZROC1zMECg{txO Ml7oNlQhcA>? NXm2UJhb\mm|ZYTpckB{cWewaX\pZ4FvfGy7IHnubIljcXSnZDDj[YxtKH[rYXLpcIl1gSCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meiBqc3nncolncWOjboTsfUB4cGWwIITo[UBkd26lZX70doF1cW:wIHnzJIhq\2incjD0bIFvKDJyIN88UUk> NHfjZY0zQTNzMkWyNC=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-IKKα/β / IKKβ / IKKα / p-IκBα / IκBα / p-p65 / p65 ; 

PubMed: 23861901     


Similar experiments were conducted with increasing doses of fisetin (1 to 10 µM), for a 15 minutes incubation with RANKL. Blots are representative of 3 independent experiments.

XBP1s / ATF6 / CHOP / ATF4 / PERK; 

PubMed: 25016296     


A375 and 451Lu cells were treated with varying doses of fisetin for 24 h and western blot analysis was performed. Equal loading was confirmed by reprobing for β-actin. Data shown are representative of three independent experiments.

DR3 / pNF-κB / NF-κB ; 

PubMed: 19670328     


Representative immunoblots showing expression of DR3, pNF-κB/p65 and NF-κB/p65 in cells treated with fisetin (0-40 μM).

p-mTOR / mTOR / Raptor / Rictor ; 

PubMed: 20530556     


Effect of fisetin on mTOR, Raptor and Rictor shown by western blot.

23861901 25016296 19670328 20530556
Immunofluorescence
p-4EBP1 / p-eIF4B / p-rpS6 ; 

PubMed: 20530556     


Effect of fisetin on mTOR target proteins. Immunofluorescent imaging of mTOR target proteins upon fisetin treatment is shown. Cells were seeded and cultured for 24 h in two-chamber glass slides followed by treatment with indicated doses of fisetin for 48 h before fixing and labeling with antibodies. For labeling, anti-rabbit antibodies against (A) p-4EBP1 (S65), (B) p-eIF4B (S422) and (C) p-rpS6 (S235/S236) (all from Cell Signaling Technology) were used. Alexa Fluor goat and anti-rabbit IgGs (Invitrogen) were used as secondary antibodies. 4′,6-Diamidino-2-phenylindole (DAPI) was used to counter stain the nucleus; ×600.

uPA ; 

PubMed: 23940799     


Cells were fixed, permeabilized, and immunostained with anti-uPA antibody (red), and cell nuclei were counter-stained with DAPI reagent. 

20530556 23940799
Growth inhibition assay
Cell viability; 

PubMed: 19670328     


Cell growth and viability of human PaC AsPC-1cells treated with fisetin (0-80 μM for 24 and 48 h) as measured by MTT assay. Each concentration of fisetin was repeated in ten wells. The values are represented as percent viable cells where vehicle (DMSO)-treated cells were regarded as 100% viable. Data is represented as mean ± SE of three independent experiments. Inset: Structure of fisetin.

19670328

Protocol

Solubility (25°C)

In vitro DMSO 57 mg/mL (199.13 mM)
Ethanol 3 mg/mL (10.48 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 286.24
Formula

C15H10O6

CAS No. 528-48-3
Storage powder
in solvent
Synonyms N/A

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Sirtuin Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID