AGK2

Catalog No.S7577

For research use only.

AGK2 is a potent, and selective SIRT2 inhibitor with IC50 of 3.5 μM that minimally affects either SIRT1 or SIRT3 at 10-fold higher levels.

AGK2 Chemical Structure

CAS No. 304896-28-4

Selleck's AGK2 has been cited by 21 publications

Purity & Quality Control

Choose Selective Sirtuin Inhibitors

Other Sirtuin Products

Biological Activity

Description AGK2 is a potent, and selective SIRT2 inhibitor with IC50 of 3.5 μM that minimally affects either SIRT1 or SIRT3 at 10-fold higher levels.
Targets
SIRT2 [1]
(Cell-free assay)
3.5 μM
In vitro

In SIRT2-myc-expressing HeLa cells, AGK2 effectively inhibits the activity of SIRT2, and increases acetylated tubulin. AGK2 protects dopaminergic neurons from α-Syn–induced toxicity in primary midbrain cultures. [1] AGK2 induces both necrosis and caspase-3-dependent apoptosis in C6 glioma cells. [2] SIRT2 also decreases merlin-mutant viability of mouse schwann cells (MSCs) without substantially reducing wild-type MSC viability. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa M{DPRmZ2dmO2aX;uJIF{e2G7 NXXWeI5ZUW6qaXLpeIlwdiCxZjDveoVzNWW6cILld5Nm\CCVSWLUNkBqdW23bn;wdoVkcXCrdHH0[YQh\nKxbTDI[WxiKGOnbHzzJJRz[W6|ZnXjeIVlKHerdHigV2lTXDJvbYnjJIV5eHKnc4Ppc44h[2:wc4TyeYN1 NUXDWYtnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PFg6ODBpPkG3OVg5QTByPD;hQi=>
H4 MYjGeY5kfGmxbjDhd5NigQ>? NV7GOZNjOjRiaILz NUTWR4gzUW6qaXLpeIlwdiCxZjDTTXJVOiCrbjDIOEBk\WyuczDjc{11emGwc3\lZ5Rm\CC5aYToJIFteGijU4nuJIFv\CC|eX7wbIltcW5zIHHzd4V{e2WmIHHzJIVvdGG{Z3XkJIFteGijU4nuJIlv[2y3c3nvcpMh[W[2ZYKgNlQhcHK| NEHLTG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W4PFkxOCd-MUe1PFg6ODB:L3G+
rat embryo midbrain MmTvSpVv[3Srb36gZZN{[Xl? MXvQdo91\WO2aX;uJI9nKGSxcHHtbY5memerYzDj[YxtKGSnYYToJIlvKHKjdDDlcYJzgW9ibXnkZpJicW5idILhcpNlfWOnZDD3bZRpKGynboTpeolzfXNiZX7jc4RqdmdiYXzwbIFUgW5vQUWzWEBufXSjboSgZZN{\XO|ZXSgZZMhfHm{b4PpcoUhcHmmcn;4fYxie2VicH;zbZRqfmViY3XscJM> MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV6OEmwNEc,OTd3OEi5NFA9N2F-
HCT116 MUfGeY5kfGmxbjDhd5NigQ>? Ml7rNVAhfU1? MYe4JIhzew>? M1vCfWlvcGmkaYTpc44hd2ZiU1nSWFIhcW5iaIXtZY4hUEOWMUG2JINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGGlZYT5cIF1\WRiYXzwbIEufHWkdXzpckBifCBzMDD1UUBi\nSncjC4JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> Mn7IQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4NEKzNFAoRjJ{NkSyN|AxRC:jPh?=
Escherichia coli cells NYjyfVRUTnWwY4Tpc44h[XO|YYm= MXHJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KFOLUmSyJIV5eHKnc4Pl[EBqdiCHc3Po[ZJq[2irYTDjc4xqKGOnbHzzJJV{cW6pIHHj[ZR6dGG2ZXSgUJl{KHOrZHWgZ4hicW5iYX3pco8h[WOrZIOgN|c6NTN6MjCoRZJoNUircz3MfZMuVHm|KFHjLUkheDV|IHPvcop2\2G2ZXSge4l1cCCjbXnuc41mfGi7bHPveY1iemmwIHHzJJN2[nO2cnH0[UBjgSCobIXvdoV{[2WwY3WgZZN{[XluIFnDOVA:PS53zszN MmfBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7M{G1NlYoRjJ{OUOxOVI3RC:jPh?=
30PT NWDaS5JlS3m2b4TvfIlkcXS7IHHzd4F6 NUT3[IJ7PzJiaILz MXTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjCzNHBVKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDD[YxtNXSrdHXyJGdNdyCjc4PhfUwhS0N3ME25MlbPxE1? NV\yRWprRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5N|E2OjZpPkKyPVMyPTJ4PD;hQi=>
30P NVzOXpVRS3m2b4TvfIlkcXS7IHHzd4F6 MX23NkBpenN? NWX2WoNmS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hOzCSIHPlcIx{KGGodHXyJFczKGi{czDifUBE\WyuLYTpeIVzKEeObzDhd5NigSxiQ1O1NF0yOi53zszN M{Ti[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUOxOVI3Lz5{MkmzNVUzPjxxYU6=
Escherichia coli cells Mn\xSpVv[3Srb36gZZN{[Xl? MWTJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KFOLUmSxJIV5eHKnc4Pl[EBqdiCHc3Po[ZJq[2irYTDjc4xqKGOnbHzzJJV{cW6pIHHj[ZR6dGG2ZXSgUJl{KHOrZHWgZ4hicW5iYX3pco8h[WOrZIOgN|c6NTN6MjCoRZJoNUircz3MfZMuVHm|KFHjLUkheDV|IHPvcop2\2G2ZXSge4l1cCCjbXnuc41mfGi7bHPveY1iemmwIHHzJJN2[nO2cnH0[UBjgSCobIXvdoV{[2WwY3WgZZN{[XluIFnDOVA:OzkQvF2= MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl|MUWyOkc,OjJ7M{G1NlY9N2F-
30PT M2PWfGN6fG:2b4jpZ4l1gSCjc4PhfS=> NIT1O4s4OiCqcoO= M3vnV2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJFMxWFRiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JINmdGy2aYTldk1odG9ibIXtbY5me2OnboSgZZN{[XluIFPDOVA:QS54zszN NE\wTFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G4PVk3Pyd-MkOxPFk6Pjd:L3G+
30P NWLyNYZmS3m2b4TvfIlkcXS7IHHzd4F6 MlzJO|IhcHK| NITUVVZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckA{OFBiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JINmdGy2aYTldk1odG9ibIXtbY5me2OnboSgZZN{[XluIFPDOVA:OTJwNd88US=> NV;zbYdjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxPFk6PjdpPkKzNVg6QTZ5PD;hQi=>
Escherichia coli BL21 (DE3) NF3NcGpHfW6ldHnvckBie3OjeR?= NIHxU4pKdmirYnn0bY9vKG:oIH\1cIwhdGWwZ4ToJIh2dWGwIGPJVnQzKGW6cILld5Nm\CCrbjDFd4Np\XKrY3jpZUBkd2yrIFLMNlEhMESHMzmgZ4VtdHNidYPpcoch\my3b4Lv[4VvcWNiNz3hcYlvdy12LX3leIh6dGOxdX3hdolvKCiDTVOpMYxi[mWuZXSgdIVxfGmmZTDifUBndHWxcnXzZ4Vv[2ViYYPzZZktKEmFNUC9NU42Ps7:TR?= MlnaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{N{W4NlQoRjJ3Mke1PFI1RC:jPh?=
SH-SY5Y MlW3UoV2em:ycn;0[YN1cX[nIHHzd4F6 M1\5XlEhfG9iMUCgeW0> MUWzNEBucW6| M1roN25mfXKxcILveIVkfGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IH;sbYdwdWW{aXOgZYxxcGFvc4nueYNt\WmwLXnu[JVk\WRiY4n0c5RwgGmlaYT5JIlvKGi3bXHuJHNJNVO\NWmgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4h[2WubDDndo94fGhiYYSgNUB1dyBzMDD1UUBxemWrbnP1ZoF1\WRiZn;yJFMxKG2rboOg[o9tdG:5ZXSgZpkh[WyyaHGtd5lvfWOuZXnuJIFl\Gm2aX;uJI1m[XO3cnXkJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYm= M4D5fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUiyNlM1Lz5{Nkm4NlI{PDxxYU6=
MCF7 MknhSpVv[3Srb36gZZN{[Xl? NULUSVZYOTBidH:gOVAhfU1? M3izRlI1KGi{cx?= NHfP[GVKdmirYnn0bY9vKG:oIGPJVnQzKGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4h[WyyaHGteJVjfWyrbjDLOFAh[WOndInsZZRqd25ibHX2[Ywh[XRiMUCgeI8hPTBidV2gZYZ1\XJiMkSgbJJ{KGmwIIDy[ZNmdmOnIH;mJHRUSSCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> M{DYb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MUO1NFg3Lz5{OEGzOVA5PjxxYU6=
U373 NV7p[YtESW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NWf1WWloPzJiaILz MX\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFV|N{OgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF01Py54zszN NXi5ZYZPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0O|U{OzBpPkK4OFc2OzNyPD;hQi=>
Hs683 MYHD[YxtKGO7Y3zlJIF{e2G7 NYO1T2NjOjRidH:gOFghcHK| MoLnR4VtdCCleXPs[UBienKnc4SgbY4hcHWvYX6gTJM3QDNiY3XscJMh[XO|ZYPz[YQh[XNiYXPjeY12dGG2aX;uJIF1KHO3Yj3HNUBxcGG|ZTDheEBKSzVyIH\vdkBOXFRiYYPzZZkh[W[2ZYKgNlQhfG9iNEigbJJ{KGK7IIDyc5Bq\Gm3bTDpc4Rq\GVic4ThbY5qdmdvYnHz[YQh\myxdzDjfZRwdWW2com= MmLqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2N{WzN|AoRjJ6NEe1N|MxRC:jPh?=
U373 MoHJR4VtdCCleXPs[UBie3OjeR?= M2L4[|I1KHSxIES4JIhzew>? MUPD[YxtKGO7Y3zlJIFzemW|dDDpckBpfW2jbjDVN|c{KGOnbHzzJIF{e2W|c3XkJIF{KGGlY4XteYxifGmxbjDheEB{fWJvR{GgdIhie2ViYYSgTWM2OCCob4KgUXRVKGG|c3H5JIFnfGW{IEK0JJRwKDR6IHjyd{BjgSCycn;wbYRqfW1iaX;kbYRmKHO2YXnubY5oNWKjc3XkJIZtd3diY4n0c41mfHK7 MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR5NUOzNEc,Ojh2N{WzN|A9N2F-
Hs683 MnO3R4VtdCCleXPs[UBie3OjeR?= NXzufZprOjRidH:gOFghcHK| NXTUdJVIS2WubDDjfYNt\SCjcoLld5QhcW5iaIXtZY4hUHN4OEOgZ4VtdHNiYYPz[ZN{\WRiYYOgZYNkfW23bHH0bY9vKGG2IFeyM20heGijc3WgZZQhUUN3MDDh[pRmeiB{NDD0c{A1QCCqcoOgZpkheHKxcHnkbZVuKGmxZHnk[UB{fGGrbnnu[{1j[XOnZDDmcI94KGO7dH;t[ZRzgQ>? MoTKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2N{WzN|AoRjJ6NEe1N|MxRC:jPh?=
U373 MWTD[YxtKGO7Y3zlJIF{e2G7 M2PNflI1KHSxIES4JIhzew>? MW\D[YxtKGO7Y3zlJIFzemW|dDDpckBpfW2jbjDVN|c{KGOnbHzzJIF{e2W|c3XkJIF{KGGlY4XteYxifGmxbjDheEBIOi:PIIDoZZNmKGG2IFnDOVAh[W[2ZYKgNlQhfG9iNEigbJJ{KGK7IIDyc5Bq\Gm3bTDpc4Rq\GVic4ThbY5qdmdvYnHz[YQh\myxdzDjfZRwdWW2com= MkL6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2N{WzN|AoRjJ6NEe1N|MxRC:jPh?=
In vivo In drosophila model of parkinson's disease, AGK2 rescues α-Syn–mediated toxicity and modify aggregation. [1]

Protocol (from reference)

Animal Research:

[1]

  • Animal Models: Drosophila model of parkinson
  • Dosages: 1mM
  • Administration: --
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 10 mg/mL warmed
(23.02 mM)
Water Insoluble
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
8% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.

1.4mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 434.27
Formula

C23H13Cl2N3O2

CAS No. 304896-28-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC2=C(C=CC=N2)C(=C1)NC(=O)C(=CC3=CC=C(O3)C4=C(C=CC(=C4)Cl)Cl)C#N

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy AGK2 | AGK2 supplier | purchase AGK2 | AGK2 cost | AGK2 manufacturer | order AGK2 | AGK2 distributor