AGK2

Catalog No.S7577

For research use only.

AGK2 is a potent, and selective SIRT2 inhibitor with IC50 of 3.5 μM that minimally affects either SIRT1 or SIRT3 at 10-fold higher levels.

AGK2 Chemical Structure

CAS No. 304896-28-4

Selleck's AGK2 has been cited by 23 publications

Purity & Quality Control

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Biological Activity

Description AGK2 is a potent, and selective SIRT2 inhibitor with IC50 of 3.5 μM that minimally affects either SIRT1 or SIRT3 at 10-fold higher levels.
Targets
SIRT2 [1]
(Cell-free assay)
3.5 μM
In vitro

In SIRT2-myc-expressing HeLa cells, AGK2 effectively inhibits the activity of SIRT2, and increases acetylated tubulin. AGK2 protects dopaminergic neurons from α-Syn–induced toxicity in primary midbrain cultures. [1] AGK2 induces both necrosis and caspase-3-dependent apoptosis in C6 glioma cells. [2] SIRT2 also decreases merlin-mutant viability of mouse schwann cells (MSCs) without substantially reducing wild-type MSC viability. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa MXrGeY5kfGmxbjDhd5NigQ>? NGr6Z29KdmirYnn0bY9vKG:oIH;2[ZIu\XiycnXzd4VlKFOLUmSyJIludXWwb4Dy[YNqeGm2YYTl[EBnem:vIFjlUIEh[2WubIOgeJJidnOoZXP0[YQhf2m2aDDTTXJVOi2veXOg[ZhxemW|c3nvckBkd26|dIL1Z5Q> MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV6OEmwNEc,OTd3OEi5NFA9N2F-
H4 NHvpS5NHfW6ldHnvckBie3OjeR?= Mln6NlQhcHK| MkLRTY5pcWKrdHnvckBw\iCVSWLUNkBqdiCKNDDj[YxteyClbz30doFve2[nY4Tl[EB4cXSqIHHsdIhiW3mwIHHu[EB{gW6yaHnsbY4yKGG|c3Xzd4VlKGG|IHXucIFz\2WmIHHsdIhiW3mwIHnuZ4x2e2mxboOgZYZ1\XJiMkSgbJJ{ NXn0cmhzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PFg6ODBpPkG3OVg5QTByPD;hQi=>
rat embryo midbrain MnTLSpVv[3Srb36gZZN{[Xl? M3PodHBzd3SnY4Tpc44hd2ZiZH;wZY1qdmW{Z3njJINmdGxiZHXheIghcW5icnH0JIVu[nK7bzDtbYRjemGrbjD0doFve2S3Y3XkJJdqfGhibHXueIl3cXK3czDlcoNw\GmwZzDhcJBp[VO7bj3BOVNVKG23dHHueEBie3Onc4Pl[EBieyC2eYLvd4lv\SCqeXTyc5h6dGG|ZTDwc5NqfGm4ZTDj[Yxtew>? MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV6OEmwNEc,OTd3OEi5NFA9N2F-
HCT116 M2nxNmZ2dmO2aX;uJIF{e2G7 NVX5UWdsOTBidV2= NVraTHZJQCCqcoO= M1j3[GlvcGmkaYTpc44hd2ZiU1nSWFIhcW5iaIXtZY4hUEOWMUG2JINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGGlZYT5cIF1\WRiYXzwbIEufHWkdXzpckBifCBzMDD1UUBi\nSncjC4JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> NWXFeHdYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2OFI{ODBpPkKyOlQzOzByPD;hQi=>
Escherichia coli cells NEnxPGJHfW6ldHnvckBie3OjeR?= Mk\5TY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCVSWLUNkBmgHC{ZYPz[YQhcW5iRYPjbIVzcWOqaXGgZ49tcSClZXzsd{B2e2mwZzDhZ4V1gWyjdHXkJGx6eyC|aXTlJINp[WmwIHHtbY5wKGGlaXTzJFM4QS1|OEKgLGFz\y2KaYOtUJl{NUy7czjBZ{kqKHB3MzDjc45rfWejdHXkJJdqfGhiYX3pco9u\XSqeXzjc5Vu[XKrbjDhd{B{fWK|dILheIUh[nliZnz1c5Jme2OnbnPlJIF{e2G7LDDJR|UxRTVwNd88US=> MkT4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7M{G1NlYoRjJ{OUOxOVI3RC:jPh?=
30PT MX7DfZRwfG:6aXPpeJkh[XO|YYm= MVO3NkBpenN? MYjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjCzNHBVKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDD[YxtNXSrdHXyJGdNdyCjc4PhfUwhS0N3ME25MlbPxE1? MnfxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7M{G1NlYoRjJ{OUOxOVI3RC:jPh?=
30P NU\QXFhLS3m2b4TvfIlkcXS7IHHzd4F6 MWi3NkBpenN? MmT4R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gN|BRKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDD[YxtNXSrdHXyJGdNdyCjc4PhfUwhS0N3ME2xNk42|ryP M{H1WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUOxOVI3Lz5{MkmzNVUzPjxxYU6=
Escherichia coli cells NFvlV3JHfW6ldHnvckBie3OjeR?= MWTJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KFOLUmSxJIV5eHKnc4Pl[EBqdiCHc3Po[ZJq[2irYTDjc4xqKGOnbHzzJJV{cW6pIHHj[ZR6dGG2ZXSgUJl{KHOrZHWgZ4hicW5iYX3pco8h[WOrZIOgN|c6NTN6MjCoRZJoNUircz3MfZMuVHm|KFHjLUkheDV|IHPvcop2\2G2ZXSge4l1cCCjbXnuc41mfGi7bHPveY1iemmwIHHzJJN2[nO2cnH0[UBjgSCobIXvdoV{[2WwY3WgZZN{[XluIFnDOVA:OzkQvF2= NXHnboJbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5N|E2OjZpPkKyPVMyPTJ4PD;hQi=>
30PT MYnDfZRwfG:6aXPpeJkh[XO|YYm= NITSd2I4OiCqcoO= NVq4bFA3S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hOzCSVDDj[YxteyCjZoTldkA4OiCqcoOgZpkh[2WubITpeIVzNWeubzDseY1qdmW|Y3XueEBie3OjeTygR2M2OD17LkdOwG0> M1yzWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MUi5PVY4Lz5{M{G4PVk3PzxxYU6=
30P Mn;lR5l1d3SxeHnjbZR6KGG|c3H5 MX23NkBpenN? NW\telk5S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hOzCSIHPlcIx{KGGodHXyJFczKGi{czDifUBk\WyudHn0[ZIu\2yxIHz1cYlv\XOlZX70JIF{e2G7LDDDR|UxRTF{LkZOwG0> MoDKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzOEm5OlcoRjJ|MUi5PVY4RC:jPh?=
Escherichia coli BL21 (DE3) Ml\qSpVv[3Srb36gZZN{[Xl? NVjqWIV6UW6qaXLpeIlwdiCxZjDmeYxtKGynbnf0bEBpfW2jbjDTTXJVOiCneIDy[ZN{\WRiaX6gSZNkcGW{aXPobYEh[2:uaTDCUFIyKCiGRUOpJINmdGy|IIXzbY5oKG[udX;yc4dmdmmlIEetZY1qdm9vND3t[ZRpgWylb4XtZZJqdiBqQV3DLU1t[WKnbHXkJJBmeHSrZHWgZpkh\my3b4Lld4NmdmOnIHHzd4F6NCCLQ{WwQVEvPTcQvF2= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ5NUiyOEc,OjV{N{W4NlQ9N2F-
SH-SY5Y NG\LVHdP\XW{b4Dyc5Rm[3SrdnWgZZN{[Xl? M2rYbVEhfG9iMUCgeW0> M3HXRVMxKG2rboO= NYDsbVRLVmW3cn;wdo91\WO2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sgc4xq\2:vZYLpZ{BidHCqYT3zfY52[2ynaX6tbY5lfWOnZDDjfZRwfG:6aXPpeJkhcW5iaIXtZY4hW0hvU2m1XUBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiClZXzsJIdzd3e2aDDheEAyKHSxIEGwJJVOKHC{ZXnuZ5Vj[XSnZDDmc5IhOzBibXnud{Bnd2yub4fl[EBjgSCjbIDoZU1{gW63Y3zlbY4h[WSmaYTpc44hdWWjc4Xy[YQh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfS=> M1fDVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUiyNlM1Lz5{Nkm4NlI{PDxxYU6=
MCF7 NUXMVndJTnWwY4Tpc44h[XO|YYm= NV;mTIZCOTBidH:gOVAhfU1? NF2zfI8zPCCqcoO= M1\POmlvcGmkaYTpc44hd2ZiU1nSWFIhcW5iaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBidHCqYT30eYJ2dGmwIFu0NEBi[2W2eXzheIlwdiCuZY\lcEBifCBzMDD0c{A2OCC3TTDh[pRmeiB{NDDodpMhcW5icILld4Vv[2Vib3[gWHNCKGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> NF\4W4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEGzOVA5Pid-MkixN|UxQDZ:L3G+
U373 MoDhRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NVK5W2M6PzJiaILz Mn33RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCXM{ezJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:PDdwNt88US=> NVjsZZd5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0O|U{OzBpPkK4OFc2OzNyPD;hQi=>
Hs683 M13kd2NmdGxiY4njcIUh[XO|YYm= NVTJeYxMOjRidH:gOFghcHK| NILPPWRE\WyuIHP5Z4xmKGG{cnXzeEBqdiCqdX3hckBJezZ6MzDj[YxteyCjc4Pld5Nm\CCjczDhZ4N2dXWuYYTpc44h[XRic4XiMWcyKHCqYYPlJIF1KEmFNUCg[o9zKE2WVDDhd5NigSCjZoTldkAzPCC2bzC0PEBpenNiYomgdJJweGmmaYXtJIlw\GmmZTDzeIFqdmmwZz3iZZNm\CCobH;3JIN6fG:vZYTyfS=> MoXQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2N{WzN|AoRjJ6NEe1N|MxRC:jPh?=
U373 MV7D[YxtKGO7Y3zlJIF{e2G7 M4CyO|I1KHSxIES4JIhzew>? Ml;BR4VtdCCleXPs[UBienKnc4SgbY4hcHWvYX6gWVM4OyClZXzsd{Bie3Onc4Pl[EBieyCjY3P1cZVt[XSrb36gZZQhe3WkLVexJJBp[XOnIHH0JGlEPTBiZn;yJG1VXCCjc4PhfUBi\nSncjCyOEB1dyB2ODDodpMh[nlicILvdIllcXWvIHnv[Ill\SC|dHHpcolv\y2kYYPl[EBndG:5IHP5eI9u\XS{eR?= NV\qdotHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0O|U{OzBpPkK4OFc2OzNyPD;hQi=>
Hs683 MmjaR4VtdCCleXPs[UBie3OjeR?= MV[yOEB1dyB2ODDodpM> NVjxZmpNS2WubDDjfYNt\SCjcoLld5QhcW5iaIXtZY4hUHN4OEOgZ4VtdHNiYYPz[ZN{\WRiYYOgZYNkfW23bHH0bY9vKGG2IFeyM20heGijc3WgZZQhUUN3MDDh[pRmeiB{NDD0c{A1QCCqcoOgZpkheHKxcHnkbZVuKGmxZHnk[UB{fGGrbnnu[{1j[XOnZDDmcI94KGO7dH;t[ZRzgQ>? NXXKS5d[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0O|U{OzBpPkK4OFc2OzNyPD;hQi=>
U373 NVS3N3dzS2WubDDjfYNt\SCjc4PhfS=> MX[yOEB1dyB2ODDodpM> MmLaR4VtdCCleXPs[UBienKnc4SgbY4hcHWvYX6gWVM4OyClZXzsd{Bie3Onc4Pl[EBieyCjY3P1cZVt[XSrb36gZZQhTzJxTTDwbIF{\SCjdDDJR|UxKGGodHXyJFI1KHSxIES4JIhzeyCkeTDwdo9xcWSrdX2gbY9lcWSnIIP0ZYlvcW6pLXLhd4VlKG[ub4egZ5l1d22ndIL5 MnrmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2N{WzN|AoRjJ6NEe1N|MxRC:jPh?=
In vivo In drosophila model of parkinson's disease, AGK2 rescues α-Syn–mediated toxicity and modify aggregation. [1]

Protocol (from reference)

Animal Research:

[1]

  • Animal Models: Drosophila model of parkinson
  • Dosages: 1mM
  • Administration: --

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
8% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.

1.4mg/mL

Chemical Information

Molecular Weight 434.27
Formula

C23H13Cl2N3O2

CAS No. 304896-28-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC2=C(C=CC=N2)C(=C1)NC(=O)C(=CC3=CC=C(O3)C4=C(C=CC(=C4)Cl)Cl)C#N

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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