Selisistat (EX 527)

For research use only.

Catalog No.S1541 Synonyms: SEN0014196

211 publications

Selisistat (EX 527) Chemical Structure

CAS No. 49843-98-3

Selisistat (EX 527, SEN0014196) is a potent and selective SIRT1 inhibitor with IC50 of 38 nM in a cell-free assay, exhibits >200-fold selectivity against SIRT2 and SIRT3. Phase 2.

Selleck's Selisistat (EX 527) has been cited by 211 publications

Purity & Quality Control

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Biological Activity

Description Selisistat (EX 527, SEN0014196) is a potent and selective SIRT1 inhibitor with IC50 of 38 nM in a cell-free assay, exhibits >200-fold selectivity against SIRT2 and SIRT3. Phase 2.
Features Greater potency, specificity, stability, and lower toxicity than other inhibitors of SIRT1 catalytic activity identified to date.
Targets
SIRT1 [1]
(Cell-free assay)
38 nM
In vitro

EX 527 exhibits potently inhibitory effect against SIRT1 deacetylase activity in a concentration-dependent manner with an IC50 of 38 nM, displays much lower activity against SIRT2 and SIRT3 with IC50 values of 19.6 μM and 48.7 μM, respectively. EX 527 does not inhibit SIRT4-7 and class I/II HDAC activity at concentrations up to 100 μM. EX-527 alone (1 μM) has no detectable effect on the acetylation of p53 lysine 382 in NCI-H460 cells. EX-527 significantly increases the amount of acetylated p53 in NCI-H460 cells, human mammary epithelial cells, U-2 OS and MCF-7 cells subjected to genotoxic agents such as Etoposide, Doxorubicin, Hydroxyurea, and Hydrogen peroxide, which is more effective than that caused by Nicotinamide (5 mM). But surprisingly EX 527 does not result in detectable effects on p53-controled gene expression, cell survival, or cell proliferation. [1] EX 527 causes a 90% increase in cell number of HCT116 cells after 7 days in the condition of 0.1% serum but not 10% serum, suggesting that SIRT1 is a significant regulator of cell proliferation during growth factor deprivation conditions. [2] EX 527 abrogates resveratrol effects on glucose responses, and prevents resveratrol-induced up-regulation of Glut2, glucokinase, Pdx-1, and Tfam in INS-1E Cells, due to the opposite effect of EX 527 and resveratrol on SIRT1 deacetylase activity. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Platelets MWrBdI9xfG:|aYOgRZN{[Xl? NY\wbVVWOTBxNUCg{txO NVr4SnZXOTBibXnu MVfEUXNQ MYTpcoNz\WG|ZYOgVm9UKGyndnXsJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh{OUS5OUc,OjV6Mkm0PVU9N2F-
HEK 293 M4Pn[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYOyOEBp NWnyNFZYUUN3ME25O{44KMLzIEiuNUDPxE1? NF;YPIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm5PFQzPyd-MkS5PVg1Ojd:L3G+
HeLa NF:xOmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIjUNmUzPCCq MXrJR|UxRTN5LkpCpOKyyqBzLkig{txO NXrZ[Hg3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5PVg1OjdpPkK0PVk5PDJ5PD;hQi=>
HEK 293 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVvoV3pXPDhiaB?= NEWyV|RKSzVyPU[5MlDDqMLzwrCwMlch|ryP M3;SbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUm4OFI4Lz5{NEm5PFQzPzxxYU6=
HeLa NY[ySGZvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3XKSlQ5KGh? MUfJR|UxRThwOdMgxtHDqDFwOTFOwG0> MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl7OESyO{c,OjR7OUi0Nlc9N2F-
RMECs MmX4RZBweHSxc3nzJGF{e2G7 NV3ldG1IOTEEoN88US=> NHPuO40zPMLiaB?= Mor5ZZR1\W63YYTld{B1cGViYX70bU1ieG:ydH;0bYMh\W[oZXP0JI9nKESncz3H Ml7nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2OE[xOFcoRjJ2NEi2NVQ4RC:jPh?=
HUVECs MVrBdI9xfG:|aYOgRZN{[Xl? NXq5cZNJOTEEoN88US=> NXP5c3pJOjRiaB?= M1fIdoJwdGm|aHXzJJRp\SCycn;0[YN1cX[nIHXm[oVkfCCxZjDy[ZN3\XKjdILvcEBqdiClZXzsJJZq[WKrbHn0fS=> M4LsVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{W4PVI5Lz5{M{O1PFkzQDxxYU6=
K562 NYf0bIlnTnWwY4Tpc44hSXO|YYm= NWjuOJByOC5zLUGg{txO NYjReZFUOiCq NYLlfWNle3SrbYXsZZRmeyCQcn[yMYRmeGWwZHXueEBo\W6nIITyZY5{[3KrcITpc44> MnHvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFzOU[0PVcoRjJzMUm2OFk4RC:jPh?=
INS-1E MkjrSpVv[3Srb36gRZN{[Xl? NHrjcXEyKM7:bR?= MXuyOEBp MXrwdoV3\W62czDy[ZN3\XKjdILvcE1qdmS3Y3XkJJVxNXKnZ4XsZZRqd25ib3dCpGdtfXR{LDDncJVkd2urbnHz[UxR\HhvMTygZY5lyqCWZnHt MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTF4M{m0Okc,OjFzNkO5OFY9N2F-
MCF-7  NYm0SG5rT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWDuVZlNOC1zMECg{txO NV:4fVBsOjRxNEivO|IhcA>? NH;MT5VFVVOR MX7y[ZBz\XO|ZYOgZ4VtdCCycn;sbYZmemG2aX;uJIF1KHSqZTDjc45k\W62cnH0bY9vKOLLpUGwNEDPxE1? NXfTPXV7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCzO|E4ODlpPkKwN|cyPzB7PD;hQi=>
NCI-H460  MoXaSpVv[3Srb36gRZN{[Xl? MlzSNUDPxG1? MVy2JIg> MUfEUXNQ MXfwdo9lfWOnczDhJINwdmOnboTyZZRqd25vZHXw[Y5l\W62IHnuZ5Jm[XOnIHnuJJRp\SCjbX;1cpQhd2ZiYXPleJlt[XSnZDDwOVM> MoH3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ|NUS2O|coRjF4M{W0Olc4RC:jPh?=
293T MV;GeY5kfGmxbjDhd5NigQ>? MYDJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEeVVD30ZYdo\WRiU1nSWFEh\XiycnXzd4VlKGmwIEK5N3Qh[2WubIOgZpkhTmy3b4Kg[IUhVHm|IH\seY9z\XOlZX7j[UBie3OjeTygTWM2OCB;IECuNFM5KM7:TT6= M2HpS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{C2PVA3Lz5{MUOwOlkxPjxxYU6=
Escherichia coli cells MXXGeY5kfGmxbjDhd5NigQ>? MWTJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KFOLUmSxJIV5eHKnc4Pl[EBqdiCHc3Po[ZJq[2irYTDjc4xqKGOnbHzzJJV{cW6pIHHj[ZR6dGG2ZXSgUJl{KHOrZHWgZ4hicW5iYX3pco8h[WOrZIOgN|c6NTN6MjCoRZJoNUircz3MfZMuVHm|KFHjLUkheDV|IHPvcop2\2G2ZXSge4l1cCCjbXnuc41mfGi7bHPveY1iemmwIHHzJJN2[nO2cnH0[UBjgSCobIXvdoV{[2WwY3WgZZN{[XluIFnDOVAhRSByLkG2JO69VS5? M1m0W|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUOxOVI3Lz5{MkmzNVUzPjxxYU6=
BL21 (DE3) MnL2SpVv[3Srb36gZZN{[Xl? NXLYOpZtUW6qaXLpeIlwdiCxZjDmeYxtKGynbnf0bEBpfW2jbjDTTXJVOSCneIDy[ZN{\WRiaX6gSZNkcGW{aXPobYEh[2:uaTDCUFIyKCiGRUOpJINmdGy|IIXzbY5oKG[udX;yc4dmdmmlIEetZY1qdm9vND3t[ZRpgWylb4XtZZJqdiBqQV3DLU1t[WKnbHXkJJBmeHSrZHWgZpkh\my3b4Lld4NmdmOnIHHzd4F6NCCLQ{WwJF0hOC5{MTFOwG0v MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ5NUiyOEc,OjV{N{W4NlQ9N2F-
Namalwa cells M2G3UGZ2dmO2aX;uJIF{e2G7 NYLOTJhUOyCqcoO= MV7Jcohq[mm2aX;uJI9nKFOLUmSxMY1m\GmjdHXkJIVv\G:pZX7veZMheDV|IHTlZYNmfHmuYYPlJIFkfGm4aYT5JIlvKGi3bXHuJG5idWGud3GgZ4VtdHNiYYPz[ZN{\WRiYYOgZ49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDlcohidmOnIIC1N{Bl\WGlZYT5cIF1cW:wIITvJJR4cWOnIITo[UBj[XOjbDDs[ZZmdCCjZoTldkA{KGi{czDifUBGVEmVQTygTW5JKD1iMD62JO69VS5? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl5MUe2PUc,OjV7N{G3Olk9N2F-
BL21 (DE3) MXvGeY5kfGmxbjDhd5NigQ>? NVjzRnlVUW6qaXLpeIlwdiCxZjDmeYxtKGynbnf0bEBpfW2jbjDTTXJVOiCneIDy[ZN{\WRiaX6gSZNkcGW{aXPobYEh[2:uaTDCUFIyKCiGRUOpJINmdGy|IIXzbY5oKG[udX;yc4dmdmmlIEetZY1qdm9vND3t[ZRpgWylb4XtZZJqdiBqQV3DLU1t[WKnbHXkJJBmeHSrZHWgZpkh\my3b4Lld4NmdmOnIHHzd4F6NCCLQ{WwJF0hOS57NDFOwG0v M1PBb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Mke1PFI1Lz5{NUK3OVgzPDxxYU6=
Escherichia coli cells NHTmV3RHfW6ldHnvckBie3OjeR?= MWTJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KFOLUmSyJIV5eHKnc4Pl[EBqdiCHc3Po[ZJq[2irYTDjc4xqKGOnbHzzJJV{cW6pIHHj[ZR6dGG2ZXSgUJl{KHOrZHWgZ4hicW5iYX3pco8h[WOrZIOgN|c6NTN6MjCoRZJoNUircz3MfZMuVHm|KFHjLUkheDV|IHPvcop2\2G2ZXSge4l1cCCjbXnuc41mfGi7bHPveY1iemmwIHHzJJN2[nO2cnH0[UBjgSCobIXvdoV{[2WwY3WgZZN{[XluIFnDOVAhRSB2OD61JO69VS5? MnrrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7M{G1NlYoRjJ{OUOxOVI3RC:jPh?=
A673 MoPWdWhVWyCjc4PhfS=> MYLxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSTZ5MzDj[Yxtew>? MoL4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC M3qyU5FJXFNiYYPzZZk> M4HiW5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMW1EKGOnbHzz Ml3HQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Hs683 NXXtNJZwS2WubDDjfYNt\SCjc4PhfS=> NWfvSoxNOjRidH:gOFghcHK| NWrvfVQ4S2WubDDjfYNt\SCjcoLld5QhcW5iaIXtZY4hUHN4OEOgZ4VtdHNiYYPz[ZN{\WRiYYOgZYNkfW23bHH0bY9vKGG2IFexJJBp[XOnIHH0JGlEPTBiYX\0[ZIhOjRidH:gOFghcHK|IHL5JJBzd3CrZHn1cUBqd2SrZHWgd5RicW6rbnetZoF{\WRiZnzve{BkgXSxbXX0dpk> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR5NUOzNEc,Ojh2N{WzN|A9N2F-
HCT116 MWnGeY5kfGmxbjDhd5NigQ>? M{DoNFExKHWP M{iyNFghcHK| M4Pa[WlvcGmkaYTpc44hd2ZiU1nSWFEhcW5iaIXtZY4hUEOWMUG2JINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGGlZYT5cIF1\WRicEWzJIF1KDFyIIXNJIFnfGW{IEigbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> NXrXd5llRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2OFI{ODBpPkKyOlQzOzByPD;hQi=>
NCI-H460 M3\XXGZ2dmO2aX;uJIF{e2G7 NIHiUG43KGi{cx?= NYrGTnBWUW6qaXLpeIlwdiCxZjDTTXJVNTJiaX6gbJVu[W5iTlPJMWg1PjBiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBxPTNiZHXhZ4V1gWyjdHnvckBi\nSncjC2JIhzeyCkeTDpcY12dm:ycnXjbZBqfGG2aX;uM4ludXWwb3Lsc5R1cW6pIHHuZYx6e2m|LDDJR|UxKD1iMTFOwG0v MnPVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFIxOzFzLze+R4hGVUKOPD;hQi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
GRP78 / FASL / Bcl-2 / LC3 ; 

PubMed: 29312612     


Western blot analysis of GRP78, FASL and LC3II after 0.5 mg/ml SB or 1 μM EX527 treatment of CL1-5 cells for 24 h. 

Ac-H3K9 / Fibronectin / Collagen 1 / α-SMA; 

PubMed: 24833701     


NRK-49F cells were treated with EX527 (0–100 μM) for 36 hours. Then, cell lysates were prepared and subjected to immunoblot analysis with antibodies for acetyl-H3K9 (Ac-H3K9), α-SMA, collagen I, fibronectin, or glyceraldehyde-3-phosphate dehydrogenase.

PCNA / Cyclin D1 / Cyclin E; 

PubMed: 24833701     


NRK-49F cells were cultured in medium with 5% fetal bovine serum and treated with EX527 (0–100 μM) for 36 hours. Then, cell lysates were prepared and subjected to immunoblot analysis with antibodies for PCNA, cyclin D1, cyclin E, or glyceraldehyde-3-phosp䲧疝Ỵ疞㧀疜膉痘 

pEGFR / EGFR / pPDGFRβ / PDGFRβ; 

PubMed: 24833701     


Cultured NRK-49F cells were treated with EX527 (0–100 μM) for 36 hours. Cell lysates were prepared and subjected to immunoblot analysis with antibodies for phospho-EGFR (pEGFR; Tyr1068), phospho-PDGFRβ (pPDGFRβ; Tyr751), EGFR, PDGFRβ, glyceraldehyde-3-pho䲧疝Ỵ疞㧀疜膉痘 瘿뙠ෆᾰƌෆĀ 㺣痖

pSTAT3 / STAT3 ; 

PubMed: 24833701     


NRK-49F cells were cultured in medium with 5% fetal bovine serum (FBS) and then treated with EX527 (0–100 μM) for 36 hours. After treatment, cell lysates were prepared and subjected to immunoblot analysis with antibodies for phospho-STAT3 (pSTAT3; Tyr705)䲧疝Ỵ疞㧀

p27 ; 

PubMed: 25143434     


A & B. SIRT1 inhibition with SIRT1 inhibitors upregulates p27 expression. H1299 (A) and H460 (B) cells were treated with Ex527 1 uM, Sirtinol 100 uM or Nicotinamide 10 mM for 12 hrs. Immunoblot analysis was performed with p27kip1 and β-actin antibodies.

FOXO3a / SIRT1 / Ac-p53 / p16(INK4a) ; 

PubMed: 31223423     


Whole cell lysates were subjected to western blot analysis for determining protein levels of FOXO3a, SIRT1, acetylated p53, p16INK4a, and p21 in senescent EPCs (endothelial progenitor cells). 

29312612 24833701 25143434 31223423
Immunofluorescence
p-p38; 

PubMed: 26824501     


M-P. Subcellular localization of hp-p38 in Bel-7402 (M-N) and SMMC-7721 (O-P) cells treated with identical amounts of either DMSO or EX527 at a final concentration of 50 μM for 24 h. Scale bar, 50 μm.

26824501
Growth inhibition assay
Cell viability ; 

PubMed: 24484175     


Growth inhibitory effect of EX527 on pancreatic cancer cell lines but not 293T cells. PANC-1, BXPC-3, ASPC-1, and 293T cells were exposed to different concentrations of EX527 for 48 h, and MTT assay was used to determine cell viability.

24484175
In vivo Administration of EX 527 (~10 μg) to rats increases hypothalamic acetyl-p53 levels by inhibiting hypothalamic SIRT1 activity. Co-administration of EX 527 with ghrelin markedly blunts the orexigenic action of ghrelin by decreasing the pAMPK levels, increasing the ACC levels, and abolishing the higher expression of the transcription factors FoxO1, pCREB, and Bsx and the neuropeptides NPY and AgRP in the hypothalamic arcuate nucleus. [4]

Protocol

Kinase Assay:[1]
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Inhibition of GST-SIRT1 deacetylase activity:

293T cells are transiently transfected with GST-tagged human SIRT1 in the pDEST27 Gateway vector using FuGENE-6. After 48 hours, the cells are lysed with 50 mM Tris, pH 8.0, 120 mM NaCl, 1 mM EDTA, and 0.5% Nonidet P-40, supplemented with Complete Mini protease inhibitor cocktail tablets. GST-SIRT1 is purified from lysates using glutathione-Sepharose beads and washed extensively in the above buffer. The deacetylation assay is performed with approximately 30 ng of GST-SIRT1 in the presence of EX 527 (48 pM to 100 μM). Deacetylation is measured using the Fluor de Lys kit using a fluorogenic peptide encompassing residues 379 to 382 of p53, acetylated on lysine 382. The acetylated lysine residue is coupled to an aminomethylcoumarin moiety. The peptide is deacetylated by SIRT1, followed by the addition of a proteolytic developer that releases the fluorescent aminomethylcoumarin. Briefly, enzyme preparations are incubated with 170 μM NAD+ and 100 μM p53 fluorogenic peptide for 45 minutes at 37 °C followed by incubation in developer for 15 minutes at 37 °C. Fluorescence is measured by excitation at 360 nm and emission at 460 nm and enzymatic activity is expressed in relative fluorescence units.
Cell Research:[1]
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  • Cell lines: NCI-H460, MCF-7, U-2 OS and HMEC
  • Concentrations: Dissolved in DMSO, final concentration 1 μM
  • Incubation Time: 48 or 72 hours
  • Method: For viability assays, cells are treated with EX 527 for 48 hours. Cell viability is then determined using the Cell Titer-Glo luminescent assay, which measures total ATP level as an index of cell number. Luminescence is measured on a Luminoskan Ascent. For the proliferation assay, 0.5 μCi/mL of [14C]thymidine is added to the medium immediately after EX 527. Plates are counted at 48 hours (HMEC) or 72 hours (NCI-H460, MCF-7, and U-2 OS cells) in a Microbeta liquid scintillation counter. Thymidine incorporated by the cells is detected by proximity to the scintillant in the base of the Cytostar-T tissue culture plate.
    (Only for Reference)
Animal Research:[4]
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  • Animal Models: Male Sprague-Dawley rats
  • Dosages: ~5 μg/rat
  • Administration: Intracerebroventricular injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 49 mg/mL (197.01 mM)
Ethanol 18 mg/mL (72.37 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
15mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 248.71
Formula

C13H13ClN2O

CAS No. 49843-98-3
Storage powder
in solvent
Synonyms SEN0014196
Smiles C1CC(C2=C(C1)C3=C(N2)C=CC(=C3)Cl)C(=O)N

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  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01485965 Completed Drug: SEN0014196 Huntington''s Disease Siena Biotech S.p.A. November 2011 Phase 1
NCT01521832 Completed Drug: SEN0014196 Huntington''s Disease Siena Biotech S.p.A. October 2009 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    what is the extinction coefficient of S1541 WX527?

  • Answer:

    The extinction coefficient of S1541 EX-527 is 1421.650635.

Sirtuin Signaling Pathway Map

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