Home DNA Damage/DNA Repair Sirtuin inhibitor Salermide For research use only.

Salermide

Salermide is a reverse amide with a strong in vitro inhibitory effect on Sirt1 and Sirt2. Compared with Sirt1, Salermide is even more efficient at inhibiting Sirt2.

Salermide Chemical Structure

Salermide Chemical Structure

CAS: 1105698-15-4

Selleck's Salermide has been cited by 3 publications

Purity & Quality Control

Batch: S846001 DMSO] 78 mg/mL] false] Ethanol] 8 mg/mL] false] Water] Insoluble] false Purity: 99.79%
99.79

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Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CRO cells Cytotoxicity assay 72 h Cytotoxicity against human CRO cells after 72 hrs by celltiter-glo luminescent assay, CC50=6.7 μM 23189967
CRC 1.1 cells Cytotoxicity assay 72 h Cytotoxicity against human CRC 1.1 cells after 72 hrs by celltiter-glo luminescent assay, CC50=9.7 μM 23189967
30PT cells Cytotoxicity assay 72 h Cytotoxicity against human 30PT cells after 72 hrs by celltiter-glo luminescent assay, CC50=36.5 μM 23189967
30P cells Cytotoxicity assay 72 h Cytotoxicity against human 30P cells after 72 hrs by celltiter-glo luminescent assay, CC50=41 μM 23189967
MCF7 cells Proliferation assay 30 μM 24-72 h Antiproliferative activity against human MCF7 cells at 30 uM after 24 to 72 hrs 24340169
U937 cells Function assay 50 μM 45 h Induction of apoptosis in human U937 cells at 50 uM after 45 hrs by flow cytometry 23189967
MOLT4 cells Proliferation assay 25 μM 24-72 h Antiproliferative activity against human MOLT4 cells at 25 uM after 24 to 72 hrs by MTT assay 23189967
MDA-MB-231 cells Proliferation assay 25 μM 24-72 h Antiproliferative activity against human MDA-MB-231 cells at 25 uM after 24 to 72 hrs by MTT assay 23189967
RKO cells Proliferation assay 25 μM 24-72 h Antiproliferative activity against human RKO cells at 25 uM after 24 to 72 hrs by MTT assay 23189967
Click to View More Cell Line Experimental Data

Biological Activity

Description Salermide is a reverse amide with a strong in vitro inhibitory effect on Sirt1 and Sirt2. Compared with Sirt1, Salermide is even more efficient at inhibiting Sirt2.
Targets
SIRT1 [1] SIRT2 [1]
In vitro
In vitro Salermide prompts tumour-specific cell death in a wide range of human cancer cell lines. The antitumour activity of Salermide is primarily because of a massive induction of apoptosis. Salermide induces apoptosis in cancer but not in normal cells. It induces strong apoptosis without any evident effect on the cell cycle in all the cancer cell lines analysed except in non-tumorigenic MRC5 cells. The induction of apoptosis is cell-type-specific and dose-dependent[1].
Cell Research Cell lines MOLT4, MDA-MB-231 and SW480 cancer cell lines
Concentrations 0, 25, 50, 75 or 100 μM
Incubation Time 24 h
Method Cell viability is determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay as described earlier. IC50 index is calculated using four Salermide concentrations (25, 50, 75 and 100 μm) for 24 h. The percentage of apoptotic cells is determined with the FACSCalibur apparatus.
In Vivo
In vivo Salermide is well tolerated by mice at concentrations up to 100 μM. Its feeding does not produce any adverse health effects in mice as monitored by diet consumption, body-weight gain, and postural and behavioural changes[1].
Animal Research Animal Models Athymic (BALB/c, nu/nu) female nude mice
Dosages 100 μl of 100 μM
Administration i.p.

Chemical Information & Solubility

Molecular Weight 394.47 Formula

C26H22N2O2
CAS No. 1105698-15-4 SDF Download Salermide SDF
Smiles CC(C1=CC=CC=C1)C(=O)NC2=CC(=CC=C2)N=CC3=C(C=CC4=CC=CC=C43)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 78 mg/mL ( (197.73 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 8 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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