Name: 3-TYP
Brand: Selleck Chemicals
SKU: S8628
Availability: InStock
Rating: 5 (39 reviews)

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Quality Control

Batch: Purity: 99.98%

99.98

Related Targets	Akt Wnt/beta-catenin HSP PKC ROCK Microtubule Associated Integrin Bcr-Abl Actin FAK
Other Sirtuin Inhibitors	SRT1720 HCl Selisistat (EX-527) Sirtinol AGK2 OSS_128167 SRT2104 (GSK2245840) SirReal2 Thiomyristoyl Salvianolic acid B SRT2183

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
HeLa	Cytotoxicity assay		1 to 6 hrs	Cytotoxicity against human HeLa cells assessed as cell death after 1 to 6 hrs, IC50=4.7μM	22835719
SK-MEL-28	Function assay	100 uM	24 hrs	Reduction in ATP level in human SK-MEL-28 cells at 100 uM maintained in Locke's solution after 24 hrs by luciferase-based assay in absence of GF and glucose	22835719
SK-MEL-28	Function assay	100 uM	24 hrs	Increase in superoxide level in human SK-MEL-28 cells maintained in medium containing GF and glucose at 100 uM after 24 hrs by DHE-based fluorescence ssay	22835719
SK-MEL-28	Function assay	100 uM	24 hrs	Increase in superoxide level in human SK-MEL-28 cells at 100 uM maintained in Locke's solution after 24 hrs by DHE-based fluorescence assay in absence of GF and glucose	22835719
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 100 mg/mL (684.22 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 29 mg/mL

Water : 1.25 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	10%DMSO 1 90%Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.340mg/ml (22.85mM)	Taking the 1 mL working solution as an example, add 100 μL of 33.4 mg/ml clear DMSO stock solution to 900 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	6.250mg/ml (42.76mM)	Taking the 1 mL working solution as an example, add 50 μL of 125 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	12.500mg/ml (85.53mM)	Taking the 1 mL working solution as an example, add 50 μL of 250 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	146.15	Formula	C₇H₆N₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	120241-79-4	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	3-(1H-1,2,3-triazol-4-yl) pyridine			Smiles	C1=CC(=CN=C1)C2=NNN=C2

Mechanism of Action

Targets/IC₅₀/Ki	SIRT3 (cell-free) 16 nM SIRT1 (cell-free) 88 nM SIRT2 (cell-free) 92 nM
In vitro	Melatonin-induced increases in deacetylated-SOD2 expression and SOD2 activity are significantly attenuated by 3-TYP in HepG2 cells exposed to Cd (cadmium).
References	[1] https://pubmed.ncbi.nlm.nih.gov/22835719/ [2] https://pubmed.ncbi.nlm.nih.gov/26120888/ [3] https://pubmed.ncbi.nlm.nih.gov/27067422/

Tech Support

Handling Instructions

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3-TYP Sirtuin inhibitor

Chemical Structure

Molecular Weight: 146.15