SRT3025 HCl

Name: SRT3025 HCl
Brand: Selleck Chemicals
SKU: S8481
Rating: 5 (1 reviews)

For research use only.

Catalog No.S8481

1 publication

Molecular Weight(MW): 606.2

SRT3025 is an orally available small molecule activator of the SIRT1 enzyme.

Selleck's SRT3025 HCl has been cited by 1 publication

Acta Haematol, 2019, 142(3):142-148

PubMed: 31141802

Purity & Quality Control

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Biological Activity

Description

SRT3025 is an orally available small molecule activator of the SIRT1 enzyme.

Targets

SIRT1 ^[1]
(Cell-free assay)

In vitro

SRT3025 inhibits RANKL-induced osteoclast differentiation, fusion and resorptive capacity without affecting osteoclast survival. SRT3025 inhibits RANKL-induced osteoclastogenesis in bone marrow-derived macrophages (BMMs) by activating AMPK and deacetylating RelA/p65 lysine 310, critical for activation of the NF-κB signaling pathway^[2]. SRT3025 acts faster than oxymetholone to improve hematopoiesis. It does not work by direct activation of Sirt1 in hematopoietic cells. SRT3025 might suppress p21 transcription through the down-regulation of Egr1^[3]. SRT3025 is found to activate wild-type Sirt1 protein but failed to activate the E230K mutant, an activation-resistant Sirt1 protein (due to a mutation at position 230)^[4].

In vivo

SRT3025 treatment also inhibits tumor growth in Panc-1 xenografts, even though it is not as effective in inhibiting the viability of Panc-1 cells in culture. SRT3025 is well tolerated in mice and has the potential to be used in several diseases^[1]. SRT3025 administration expands HSPCs and boosts blood counts while long term SRT3025 administration does not permanently increase or decrease HSC repopulating potential^[3]. SRT3025 reduces plasma cholesterol, inflammation, and atherosclerosis in Apoe−/− mice, and it increases hepatic Ldlr expression and Pcsk9 accumulation^[4].

Protocol

Cell Research: ^[1]	- Collapse Cell lines: HPDE, Panc-1, SU86.86, Patu8988t cells Concentrations: 0-5 μM Incubation Time: 72 h Method: Cells are treated with vehicle or different concentrations of SRT3025 for 72 hours and submitted to MTT analysis. (Only for Reference)
Animal Research: ^[1]	- Collapse Animal Models: Female athymic nu/nu mice Dosages: 50-200 mg/kg Administration: oral administration (Only for Reference)

References

[4] Miranda MX, et al. Eur Heart J. 2015, 36(1):51-9.

- Collapse

Solubility (25°C)

In vitro	DMSO	100 mg/mL warmed (164.96 mM)
	Ethanol	5 mg/mL warmed (8.24 mM)
	Water	Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download SRT3025 HCl SDF

Molecular Weight	606.2
Formula	C₃₁H₃₁N₅O₂S₂.HCl
CAS No.	2070015-26-6
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	N/A
Smiles	Cl.COCCCC1=C(N=C(S1)C2=CC=CC=C2)C(=O)NC3=CC=CC=C3C4=NC5=CC(=CN=C5S4)CN6CCCC6

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)

% DMSO+ % + % Tween 80+ % ddH₂O

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Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

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Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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Computed Result

C1=C0/X	C1:	LOG(C1):
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C3=C2/X	C3:	LOG(C3):
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Molecular Weight Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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SRT3025 HCl