Home DNA Damage/DNA Repair Sirtuin inhibitor Thiomyristoyl

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Thiomyristoyl Sirtuin inhibitor

Cat.No.S8245

Thiomyristoyl (TM) is a potent and specific SIRT2 inhibitor with an IC50 of 28 nM. It inhibits SIRT1 with an IC50 value of 98 μM and does not inhibit SIRT3 even at 200 μM.
Thiomyristoyl Sirtuin inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 581.85

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Quality Control

Batch: S824501 DMSO]100 mg/mL]false]Ethanol]100 mg/mL]false]Water]Insoluble]false Purity: 99.84%
99.84

Cell Culture, Treatment & Working Concentration

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
BxPC3 Growth inhibition assay 72 hrs Growth inhibition of human BxPC3 cells after 72 hrs by CellTiter-Blue assay, GI50=13.3μM 30986062
MDA-MB-468 Growth inhibition assay 72 hrs Growth inhibition of human MDA-MB-468 cells after 72 hrs by CellTiter-Blue assay, GI50=15.7μM 30986062
NCI-H23 Growth inhibition assay 72 hrs Growth inhibition of human NCI-H23 cells after 72 hrs by CellTiter-Blue assay, GI50=16.4μM 30986062
HCT116 Function assay 5 days Inhibition of anchorage-independent cell growth in human HCT116 cells assessed as reduction in colony formation incubated for 5 days followed by compound re-addition and measured after 9 to 11 days by nitro blue tetrazolium chloride-based soft agar colony, GI50=16.7μM 30986062
A549 Growth inhibition assay 72 hrs Growth inhibition of human A549 cells after 72 hrs by CellTiter-Blue assay, GI50=17.3μM 30986062
SW948 Growth inhibition assay 72 hrs Growth inhibition of human SW948 cells after 72 hrs by CellTiter-Blue assay, GI50=19.2μM 30986062
MCF7 Growth inhibition assay 72 hrs Growth inhibition of human MCF7 cells after 72 hrs by CellTiter-Blue assay, GI50=37μM 30986062
MDA-MB-231 Growth inhibition assay 72 hrs Growth inhibition of human MDA-MB-231 cells after 72 hrs by CellTiter-Blue assay, GI50=42.8μM 30986062
HeLa Cytotoxicity assay 50 uM 72 hrs Cytotoxicity against human HeLa cells assessed as reduction in cell viability at 50 uM measured after 72 hrs by CCK8 assay 31117516
MCF7 Growth inhibition assay 15 uM 72 hrs Growth inhibition of human MCF7 cells at 15 uM measured after 72 hrs by AlamarBlue assay 31144814
Neuro2a Function assay 0.2 to 2 uM 24 hrs Induction of neurite outgrowth in mouse Neuro2a cells at 0.2 to 2 uM measured after 24 hrs by microscopic analysis 31144814
HCT116 Function assay 25 uM 6 hrs Inhibition of SIRT2 in human HCT116 cells assessed as increase in alpha-tubulin acetylation at 25 uM incubated for 6 hrs by DAPI staining-based laser scanning confocal microscopic analysis 30986062
HCT116 Function assay 50 uM 6 hrs Inhibition of SIRT2 in human HCT116 cells assessed as increase in alpha-tubulin acetylation at 50 uM incubated for 6 hrs by DAPI staining-based laser scanning confocal microscopic analysis 30986062
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Solubility

In vitro
Batch:

DMSO : 100 mg/mL (171.86 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 100 mg/mL

Water : Insoluble

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Mass Concentration Volume Molecular Weight
Dilution Calculator Molecular Weight Calculator

In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

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%
% Tween 80
% ddH2O
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Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight 581.85 Formula

C34H51N3O3S

 Storage (From the date of receipt)
CAS No. 1429749-41-6 Download SDF Storage of Stock Solutions

Synonyms TM Smiles CCCCCCCCCCCCCC(=S)NCCCCC(C(=O)NC1=CC=CC=C1)NC(=O)OCC2=CC=CC=C2

Mechanism of Action

Targets/IC50/Ki
SIRT2
(Cell-free assay)
28 nM
In vitro
Thiomyristoyl(TM) is a highly selective SIRT2 inhibitor. It cannot efficiently inhibit SIRT3, SIRT5, SIRT6, or SIRT7. In vitro, it shows great inhibition of cell viability and its cytotoxicity is relatively selective toward cancer cells. TM decreases c-Myc oncoprotein level in cancer cells, the ability of TM to decrease c-Myc abundance in different cell lines correlates with the sensitivity of the cell lines to TM.
In vivo
The anticancer effect of TM correlates with its ability to decrease c-Myc level. TM has limited effects on non-cancerous cells and tumor-free mice.
References

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06147232 Not yet recruiting
Nephropathy|Diabetic Nephropathies|Diabetes Mellitus Type 1|Albuminuria|Diabetic Complications Renal|Diabetic Complications Cardiovascular|Hypoxia
Steno Diabetes Center Copenhagen|Juvenile Diabetes Research Foundation|King''s College London|Glostrup University Hospital Copenhagen
 August 2024 Phase 4
NCT06384157 Not yet recruiting
Opioid Use Disorder
Indivior Inc.
 May 2024 Phase 2

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