MDL-28170

Catalog No.S7394 Synonyms: Calpain Inhibitor III

For research use only.

MDL-28170 (Calpain Inhibitor III) is a potent and selective calpain inhibitor of calpain that penetrates the blood-brain barrier and inhibits brain cysteine protease activity after systemic administration. MDL-28170 is also an inhibitor of γ-secretase.

MDL-28170 Chemical Structure

CAS No. 88191-84-8

Purity & Quality Control

Choose Selective Secretase Inhibitors

Biological Activity

Description MDL-28170 (Calpain Inhibitor III) is a potent and selective calpain inhibitor of calpain that penetrates the blood-brain barrier and inhibits brain cysteine protease activity after systemic administration. MDL-28170 is also an inhibitor of γ-secretase.
Targets
calpain [1] Secretase [2]
In vitro

MDL28170 significantly enhances Schwann cells (SCs) survival in vitro in response to oxidative stress induced by application of H2O2, without reducing LDH release.[3]

In vivo

MDL 28170 is a calpain inhibitor that protects against cortical neuronal damage even if the treatment is delayed until 3 h after reperfusion. However, the neuroprotective effect of this agent is less pronounced in the hippocampal CA1 sector.[1]

Protocol (from reference)

Cell Research:

[3]

  • Cell lines: Schwann cells
  • Concentrations: 50 nM, 500 nM, 5 μM, 50 μM
  • Incubation Time: 4 h
  • Method:

    To test the ability of MDL28170 to inhibit H2O2-mediated necrosis. Cells are treated with MDL28170 (50 nM, 500 nM, 5 μM, or 50 μM). After 1 h of drug pretreatment, fresh solution of drugs and H2O2 is added to the cells for the duration of the experiment. After 3 h of exposure the number of live or dead cells is assayed.

Animal Research:

[1]

  • Animal Models: Male Mongolian gerbils
  • Dosages: 50 mg/kg
  • Administration: IP

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 382.45
Formula

C22H26N2O4

Density g/mL
CAS No. 88191-84-8
Storage 2 years -80 in solvent
Smiles CC(C)C(C(=O)NC(CC1=CC=CC=C1)C=O)NC(=O)OCC2=CC=CC=C2

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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