Resveratrol (SRT501)

For research use only.

Catalog No.S1396 Synonyms: trans-Resveratrol

41 publications

Resveratrol (SRT501) Chemical Structure

CAS No. 501-36-0

Resveratrol (SRT501, trans-Resveratrol) has a wide spectrum of targets including cyclooxygenases(i.e. COX, IC50=1.1 μM), lipooxygenases(LOX, IC50=2.7 μM), kinases, sirtuins and other proteins. It has anti-cancer, anti-inflammatory, blood-sugar-lowering and other beneficial cardiovascular effects. Resveratrol induces mitophagy/autophagy and autophagy-dependent apoptosis.

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Selleck's Resveratrol (SRT501) has been cited by 41 publications

Purity & Quality Control

Choose Selective Autophagy Inhibitors

Biological Activity

Description Resveratrol (SRT501, trans-Resveratrol) has a wide spectrum of targets including cyclooxygenases(i.e. COX, IC50=1.1 μM), lipooxygenases(LOX, IC50=2.7 μM), kinases, sirtuins and other proteins. It has anti-cancer, anti-inflammatory, blood-sugar-lowering and other beneficial cardiovascular effects. Resveratrol induces mitophagy/autophagy and autophagy-dependent apoptosis.
Targets
SIRT1 [2]
(Cell-free assay)
SIRT2 [2]
(Cell-free assay)
Quinone reductase 2 [9]
(Cell-free assay)
IKK β [1]
(Cell-free assay)
COX1 [9]
(Cell-free assay)
88 nM 1 μM 1.1 μM
In vitro

Resveratrol inhibits the activity of Cyclooxygenase and lipooxygenase, PKCs and p56lck, ERK1, JNK1, p38, IKK β, Src, STAT3, Ribonucleotide Reductase, DNA polymerases α and δ, PKD, PKC α, Quinone reductase 2, and Aromatase with IC50 of from 0.035-60 μM. Resveratrol is also an activator of Adenylyl cyclase and AMPK with EC50 of 0.8 μM and 50 μM, respectively. The roles of Resveratrol as inhibitor or activator enable its effects on decreasing cell inflammatory associated behaviors, growth inhibition and induction of apoptosis in cancer cells, reversal of endothelin-1 stimulated cell responses, inhibition of phorbol ester-induced expression of COX-2, inhibition of DNA synthesis in cell, resistance to menadione-induced cell death, and improvement of cell mitochondrial function and glucose/lipid metabolism. [1] Resveratrol is also an activator of sirtuins. Resveratrol lowers the Michaelis constant of SIRT1 for both the acetylated substrate and NAD+, and increases cell survival by stimulating SIRT1-dependent deacetylation of p53. In yeast, Resveratrol mimics calorie restriction by stimulating Sir2, increasing DNA stability and extending lifespan. [2] Resveratrol is effective at protecting isolated rat hearts against ischemia/reperfusion injury via its antioxidant activity, with improved recovery of developed pressure and aortic flow, reduction of malondialdehyde concentrations and reduction of infarct size. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HL60 cell line NGPGcJhRem:uaX\ldoF1cW:wIHHzd4F6 MlPkRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKTE[wJINmdGxibHnu[UwhUUN3ME21JO69VQ>? NEfa[VY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNk[4OlU1Oyd-MU[2PFY2PDN:L3G+
human K562 cell line NEXtVYpRem:uaX\ldoF1cW:wIHHzd4F6 Ml70RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCNNU[yJINmdGxibHnu[UBmgHC{ZYPzbY5oKEKlcj3BZowtKEmFNUC9Nlgh|ryP M1ruSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4Nki2OVQ{Lz5zNk[4OlU1OzxxYU6=
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MCF7 M3PrN2Z2dmO2aX;uJIF{e2G7 NXraUpd2TXO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCPQ1[3JINmdGy|IHHzd4V{e2WmIHHzJIRzfWdibHX2[Ywh[2G3c3nu[{B{fGmvdXzheIlwdiCxZjDj[YxtKHC{b3zp[oVz[XSrb36g[ZF2cX[jbHXueEB1dyBzMDDwUUBme3S{YXTpc4wh[nliYXzhcYFzKGKudXWgZZN{[XluIFHjeIl3cXS7PUGuOu69VS5? MlLDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5OEe0N|YoRjF3N{i3OFM3RC:jPh?=
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Nanog / Oct-4 / Sox-2 ; 

PubMed: 21304978     


Pancreatic CSCs were treated with resveratrol (0–30 µM) for 48 h. The expression of Nanog, Oct-4 and Sox-2 was measured by the Western blot analysis. GAPDH was used as a loading control. 

p-EGFR / EGFR / p-HER2 / HER2 / p-ERK / ERK / PTEN ; 

PubMed: 20729295     


DU145 cells were treated with DMSO, and 10, 20 or 50 µm resveratrol. Western blots show resveratrol decreased phosphorylation of EGFR-Tyr1068 (upper panel), HER2-Tyr1221/1222 (second panel).

21304978 20729295
Growth inhibition assay
Cell viability; 

PubMed: 23272133     


A, MTS assay was performed on PC-3M-MM2 cells treated with vehicle or different concentrations of resveratrol (5–100 µM) for 72 h which showed that resveratrol dose-dependently reduced cell viability. B, Decrease in cell viability by resveratrol was time-dependent in the resveratrol-treated groups (25 and 50 µM) compared to vehicle.

23272133
In vivo Resveratrol improves health and survival of mice on a high-calorie diet. Resveratrol (22.4 mg/kg/day) shifts the physiology of middle-aged mice on a high-calorie diet towards that of mice on a standard diet and significantly increases their survival. Resveratrol produces changes associated with longer lifespan, including increased insulin sensitivity, reduced insulin-like growth factor-1 (IGF-I) levels, increased AMP-activated protein kinase (AMPK) and peroxisome proliferator-activated receptor-γcoactivator 1α(PGC-1α) activity, increased mitochondrial number, and improved motor function. Resveratrol opposed the effects of the high-calorie diet in 144 out of 153 significantly altered pathways. [3] Resveratrol has been shown to inhibit the initiation and growth of tumors in a wide variety of rodent cancer models. Dose of Resveratrol as low as 200 μg/kg daily already shows efficacy in a rat model of colon carcinogenesis. At higher dose of 40 mg/kg, Resveratrol increases the survival of mice with subcutaneous neuroblastomas from 0% to 70%. Resveratrol inhibits vascularization in the corneal micropocket assay in mice at a dose of only 48 μg/kg when administered daily. [3] Resveratrol shows beneficial effects on heart disease. Resveratrol blocks the increase in platelet aggregation induced by a hypercholesterolaemic diet. Resveratrol increase expression of both endothelial and inducible nitric oxide synthase. In stroke-prone, spontaneously hypertensive rats, resveratrol significantly reduces markers of oxidative stress such as glycated albumin in serum, and 8-hydroxyguanos-ine in urine. Providing Resveratrol in drinking water for 15 days (1 mg/kg) is sufficient to improve the recovery in function and coronary flow of isolated hearts. [3] Resveratrol displays anti-inflammatory activity in vivo. Resveratrol significantly reduces both acute and chronic chemically induced oedema, lipopolysaccharide-induced airway inflammation and osteoarthritis, and helps to prevent allograft rejection. Intravenously administered Resveratrol decreases inflammation induced by ischaemia/reperfusion, oxidants generated by hypoxanthine/xanthine oxidase (HX/XO) or platelet-activating factor, but not leukotriene B4 in rats. [3] Resveratrol shows beneficial effects on stroke and brain damage. Resveratrol administered intravenously significantly decreased ischaemic volume and brain water content at the extremely low doses of 100 ng/kg and 1 μg/kg after middle cerebral artery occlusion in rats. [5]

Protocol

Cell Research:

[6]

- Collapse
  • Cell lines: Human breast cancer cell MCF-7
  • Concentrations: 30-300 μM
  • Incubation Time: 2 days
  • Method:

    Cells are plated in flat-bottomed, 96-well microtiter plates (4000 cells/6.4-mm-diameter well). After 12–24 hr, cells are treated with DMSO (0.1–0.3%) or increasing doses of Resveratrol. After 48 h of treatment, cells are treated with 10μL of MTT reagent for 4 hr at 37 ℃ and then treated with 100 μL of solubilization solution at 37 ℃ overnight. The quantity of formazan product is measured using a spectrophotometric microtiter plate reader at 570 nm wavelength.


    (Only for Reference)
Animal Research:

[7]

- Collapse
  • Animal Models: Human ovarian xenografts PA-1
  • Dosages: 100 mg/kg
  • Administration: i.p. daily for consecutive 4 weeks
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 45 mg/mL warmed (197.16 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
15mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 228.24
Formula

C14H12O3

CAS No. 501-36-0
Storage powder
in solvent
Synonyms trans-Resveratrol
Smiles C1=CC(=CC=C1C=CC2=CC(=CC(=C2)O)O)O

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Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03743636 Recruiting Drug: Nicotinamide riboside|Drug: Resveratrol|Other: Placebo Peripheral Artery Disease Northwestern University|American Heart Association October 1 2018 Phase 3
NCT03253913 Unknown status Drug: Sirolimus|Drug: Resveratrol Lymphangioleiomyomatosis University of Cincinnati|National Heart Lung and Blood Institute (NHLBI) March 31 2018 Phase 2
NCT01476592 Completed Dietary Supplement: Resveratrol Neuroendocrine Tumor University of Wisconsin Madison December 2011 Not Applicable

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Autophagy Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID