Resveratrol (SRT501)

Catalog No.S1396 Synonyms: trans-Resveratrol

For research use only.

Resveratrol (SRT501, trans-Resveratrol) has a wide spectrum of targets including cyclooxygenases(i.e. COX, IC50=1.1 μM), lipooxygenases(LOX, IC50=2.7 μM), kinases, sirtuins and other proteins. It has anti-cancer, anti-inflammatory, blood-sugar-lowering and other beneficial cardiovascular effects. Resveratrol induces mitophagy/autophagy and autophagy-dependent apoptosis.

Resveratrol (SRT501) Chemical Structure

CAS No. 501-36-0

Selleck's Resveratrol (SRT501) has been cited by 51 publications

Purity & Quality Control

Choose Selective Autophagy Inhibitors

Biological Activity

Description Resveratrol (SRT501, trans-Resveratrol) has a wide spectrum of targets including cyclooxygenases(i.e. COX, IC50=1.1 μM), lipooxygenases(LOX, IC50=2.7 μM), kinases, sirtuins and other proteins. It has anti-cancer, anti-inflammatory, blood-sugar-lowering and other beneficial cardiovascular effects. Resveratrol induces mitophagy/autophagy and autophagy-dependent apoptosis.
Targets
SIRT1 [2]
(Cell-free assay)
SIRT2 [2]
(Cell-free assay)
Quinone reductase 2 [9]
(Cell-free assay)
IKK β [1]
(Cell-free assay)
COX1 [9]
(Cell-free assay)
Click to View More Targets
88 nM 1 μM 1.1 μM
In vitro

Resveratrol inhibits the activity of Cyclooxygenase and lipooxygenase, PKCs and p56lck, ERK1, JNK1, p38, IKK β, Src, STAT3, Ribonucleotide Reductase, DNA polymerases α and δ, PKD, PKC α, Quinone reductase 2, and Aromatase with IC50 of from 0.035-60 μM. Resveratrol is also an activator of Adenylyl cyclase and AMPK with EC50 of 0.8 μM and 50 μM, respectively. The roles of Resveratrol as inhibitor or activator enable its effects on decreasing cell inflammatory associated behaviors, growth inhibition and induction of apoptosis in cancer cells, reversal of endothelin-1 stimulated cell responses, inhibition of phorbol ester-induced expression of COX-2, inhibition of DNA synthesis in cell, resistance to menadione-induced cell death, and improvement of cell mitochondrial function and glucose/lipid metabolism. [1] Resveratrol is also an activator of sirtuins. Resveratrol lowers the Michaelis constant of SIRT1 for both the acetylated substrate and NAD+, and increases cell survival by stimulating SIRT1-dependent deacetylation of p53. In yeast, Resveratrol mimics calorie restriction by stimulating Sir2, increasing DNA stability and extending lifespan. [2] Resveratrol is effective at protecting isolated rat hearts against ischemia/reperfusion injury via its antioxidant activity, with improved recovery of developed pressure and aortic flow, reduction of malondialdehyde concentrations and reduction of infarct size. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HL60 cell line Mn3uVJJwdGmoZYLheIlwdiCjc4PhfS=> MlfERY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKTE[wJINmdGxibHnu[UwhUUN3ME21JO69VQ>? MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjZ6NkW0N{c,OTZ4OE[1OFM9N2F-
human K562 cell line NIK0TFJRem:uaX\ldoF1cW:wIHHzd4F6 MYfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEt3NkKgZ4VtdCCuaX7lJIV5eHKnc4PpcochSmO{LVHicEwhUUN3ME2yPEDPxE1? M4T0WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4Nki2OVQ{Lz5zNk[4OlU1OzxxYU6=
HUVEC MXvGeY5kfGmxbjDhd5NigQ>? MknjNVAxKM7:TR?= Ml7lNUBl[Xl? M2nkRmlv\HWldHnvckBw\iCvaYTvZ4hwdmS{aXHsJIJqd2enbnXzbZMhcW5iSELPNk1qdmS3Y3XkJGhWXkWFIHH0JFExOCC3bX;sM2wheHKndILlZZRm\CCob4KgNUBl[XliZn;scI94\WRiYomgZ49ueG:3bnSge4F{cG:3dDDhcoQhUDKRMj30doVifG2nboSgcYVie3W{ZXSgZYZ1\XJiMUCg[IF6eyCkeTDNbZRwXHKjY3vldkBT\WRic4ThbY5qdmdvYnHz[YQh[XO|YYm= NF\2Spo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i1PVI1QSd-MkO4OVkzPDl:L3G+
Hepa1clc7 M1rQTWN6fG:2b4jpZ4l1gSCjc4PhfS=> M1TScVczKGi{cx?= MUjDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDI[ZBiOWOuY{egZ4VtdHNiYX\0[ZIhPzJiaILzMEBKSzVyPUGx{txONg>? NUjiOZVURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwO|U4PDJpPkGwNFc2PzR{PD;hQi=>
MCF7 NV35cVd{TnWwY4Tpc44h[XO|YYm= NYDNNpNGPiCqcoO= M2PUTGlvcGmkaYTpc44hd2ZicHjvdoJwdCCnc4Tldk1qdmS3Y3XkJI9zdmm2aHnu[UBl\WOjcnLvfJlt[XOnIHnuJIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiNjDodpMtKEmFNUC9NVnPxE1w NH\xWmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEC3OVc1Oid-MUCwO|U4PDJ:L3G+
U937 NWjSTWJGTnWwY4Tpc44h[XO|YYm= MVrJcohq[mm2aX;uJI9nKFtzNFPd[IVpgWS{b3HzZ49z[mmlIHHjbYQhfXC2YXvlJIlvKGi3bXHuJHU6OzdiY3XscJMh[nliTXnjbIFmdGm|LV3lcpRmdiCybH;0MEBMcT1|Nd88UU4> NHns[JA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUK3O|c3PCd-MUGyO|c4PjR:L3G+
U937 Ml;YSpVv[3Srb36gZZN{[Xl? NXLxXGlYUW6qaXLpeIlwdiCxZjCyMXsyNDJvM1ioUkle\GWxeImtSE1odHWlb4PlJJVxfGGtZTDpckBpfW2jbjDVPVM4KGOnbHzzJIJ6KHOlaX70bYxt[XSrb36gd5Bm[3S{b33leJJ6KGmwIIDy[ZNmdmOnIH;mJJNw\Gm3bT3mdoVmKGK3Zn\ldi=> M3KxXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzMke3O|Y1Lz5zMUK3O|c3PDxxYU6=
HL60 M{LtSWZ2dmO2aX;uJIF{e2G7 MnLnTY5pcWKrdHnvckBw\iB{LWuxMFIuO0hqTjnd[IVwgHlvRD3ncJVkd3OnIIXweIFs\SCrbjDoeY1idiCKTE[wJINmdGy|IHL5JJNkcW62aXzsZZRqd25ic4DlZ5Rzd22ndIL5JIlvKHC{ZYPlcoNmKG:oIIPv[Il2dS2ocnXlJIJ2\m[nch?= Mn7MQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF{N{e3OlQoRjFzMke3O|Y1RC:jPh?=
U937 NULQSYFlTnWwY4Tpc44h[XO|YYm= MoP2TY5pcWKrdHnvckBw\iCdMUTDYYRmcHmmcn;hd4NwemKrYzDhZ4llKHWydHHr[UBqdiCqdX3hckBWQTN5IHPlcIx{KGK7IIPjbY51cWyuYYTpc44he3CnY4Tyc41mfHK7IHnuJJBz\XOnbnPlJI9nKHOxZHn1cU1nemWnIHL1[oZmeg>? M{nXXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzMke3O|Y1Lz5zMUK3O|c3PDxxYU6=
HL60 NFrZVnRHfW6ldHnvckBie3OjeR?= NXqyRpVLUW6qaXLpeIlwdiCxZjDbNVREZWSnaInkdo9ie2OxcnLpZ{Bi[2mmIIXweIFs\SCrbjDoeY1idiCKTE[wJINmdGy|IHL5JJNkcW62aXzsZZRqd25ic4DlZ5Rzd22ndIL5JIlvKHC{ZYPlcoNmKG:oIIPv[Il2dS2ocnXlJIJ2\m[nch?= MlXoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF{N{e3OlQoRjFzMke3O|Y1RC:jPh?=
CHO M3zseGZ2dmO2aX;uJIF{e2G7 M{P0ZWJtd2OtYXTlJI9nKHKjdDDHcJV1OSCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gNk1cOSx{LUPILG4qZWSnb4j5MWQu\2y3Y3;z[UB2eHSja3WgZpkhe2OrboTpcIxifGmxbjDzdIVkfHKxbXX0dpk> M1;FWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzMke3O|Y1Lz5zMUK3O|c3PDxxYU6=
CHO M3rjNWZ2dmO2aX;uJIF{e2G7 NIe5WmhDdG:la3Hk[UBw\iC{YYSgS4x2fDFiZYjwdoV{e2WmIHnuJGNJVyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJHsyPEOfZHXofYRzd2G|Y3;yZolkKGGlaXSgeZB1[WunIHL5JJNkcW62aXzsZZRqd25ic4DlZ5Rzd22ndIL5 M1TaRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzMke3O|Y1Lz5zMUK3O|c3PDxxYU6=
CHO NGnwdnVHfW6ldHnvckBie3OjeR?= MX3CcI9kc2GmZTDv[kBpfW2jbjDHcJV1OyCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gNk1cOSx{LUPILG4qZWSnb4j5MWQu\2y3Y3;z[UB2eHSja3WgZpkhe2OrboTpcIxifGmxbjDzdIVkfHKxbXX0dpk> M1;ndFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzMke3O|Y1Lz5zMUK3O|c3PDxxYU6=
CHO MVTGeY5kfGmxbjDhd5NigQ>? M3LTeWJtd2OtYXTlJI9nKGi3bXHuJGdtfXR|IHX4dJJme3OnZDDpckBEUE9iY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBcOTSFXXTlbJllem:jc3PvdoJq[yCjY3nkJJVxfGGtZTDifUB{[2mwdHnscIF1cW:wIIPw[YN1em:vZYTyfS=> M362O|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzMke3O|Y1Lz5zMUK3O|c3PDxxYU6=
HL60 NFy5[IhHfW6ldHnvckBie3OjeR?= Mn\4TY4hfmm2cn:gbY5pcWKrdH;yfUBkd26lZX70doF1cW:wIHHnZYlve3RicILvcIln\XKjdHnvckBw\iCKTE[wJINmdGy|LDDJR|UxRTYQvF2u MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjh5N{W5N{c,OTJ6N{e1PVM9N2F-
RAW264.7 M2PKXmZ2dmO2aX;uJIF{e2G7 MXXJcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4Sgcol1emmlIH;4bYRmKHC{b3T1Z5Rqd25iaX6gcIlxd3CxbInzZYNkcGG{aXTlMYFkfGm4YYTl[EBud3W|ZTDtZYNzd3CqYXflJHJCXzJ4ND63JINmdGy|LDDJR|UxRTF3LkpOwG0v MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDV3Mke1PUc,OTR3NUK3OVk9N2F-
RAW264.7 M2XrOGZ2dmO2aX;uJIF{e2G7 MUjJcohq[mm2b4L5JIVn\mWldDDvckBRT0VvMjDwdo9lfWO2aX;uJIlvKEySUz3zeIlufWyjdHXkJHJCXzJ4ND63JINmdGy|LDDJR|UxRTUQvF2u M1r0VFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3MEiwPVg5Lz5zNUC4NFk5QDxxYU6=
RAW264.7 NIq1fIJHfW6ldHnvckBie3OjeR?= NV3jS3E6cW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4SgVGdGNTJicILv[JVkfGmxbjDpckBNWFNvc4TpcZVt[XSnZDDSRXczPjRwNzDj[YxteyxiSVO1NF0zOC56zszNMi=> NH;NWmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUWwNVA3PCd-MUW1NFExPjR:L3G+
T47D MVTGeY5kfGmxbjDhd5NigQ>? NWTM[JlVTXO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCWNEfEJINmdGy|IHHzd4V{e2WmIHHzJIRzfWdibHX2[Ywh[2G3c3nu[{B{fGmvdXzheIlwdiCxZjDj[YxtKHC{b3zp[oVz[XSrb36g[ZF2cX[jbHXueEB1dyBzMDDwUUBme3S{YXTpc4wh[nliYXzhcYFzKGKudXWgZZN{[XluIFHjeIl3cXS7PUCuNFPPxE1w MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd6N{SzOkc,OTV5OEe0N|Y9N2F-
MCF7 M2XuRmZ2dmO2aX;uJIF{e2G7 MVfFd5Rzd2enbnnjJIFkfGm4aYT5JIlvKGi3bXHuJG1ETjdiY3XscJMh[XO|ZYPz[YQh[XNiZIL1[{Bt\X[nbDDjZZV{cW6pIIP0bY12dGG2aX;uJI9nKGOnbHygdJJwdGmoZYLheIlwdiCncYXpeoFt\W62IITvJFExKHCPIHXzeJJi\GmxbDDifUBidGGvYYKgZox2\SCjc4PhfUwhSWO2aY\peJk:OS54zszNMi=> NVnlZosxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3PFc1OzZpPkG1O|g4PDN4PD;hQi=>
MDA-MB-231 NFnvNYhCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NYPYTpY4UW6qaXLpeI9zgSClb37j[Y51emG2aX;uJJJmeXWrcnXkJIZweiCjboTpdJJwdGmoZYLheIl3\SCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOsJGlEPTB;MkCuOe69VS5? M3vCUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4MkWwOlM3Lz5zNkK1NFY{PjxxYU6=
U937 NV3YZ3pIS3m2b4TvfIlkcXS7IHHzd4F6 M3\O[mN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHU6OzdiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHdUXDFidHXzeEwhUUN3ME2xO:69VS5? MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjJ3MkmyOEc,OTZ{NUK5NlQ9N2F-
HL60 NInZNY5CdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MXvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiONkCgZ4VtdHNuIFnDOVA:Pc7:TT6= MoHOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ3OECyNFQoRjF4NUiwNlA1RC:jPh?=
HEK293T MkXZSpVv[3Srb36gZZN{[Xl? M12w[GlvcGmkaYTpc44hd2ZiVF7GMYFteGijLXnu[JVk\WRiTl[tb4FxeGGEIHHjeIl3[XSrb36gbY4hUEWNMkmzWEBk\WyuczygTWM2OD1{MN88UU4> M2rnW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MUK1NlcxLz5zN{GyOVI4ODxxYU6=
BV2 NILo[npHfW6ldHnvckBie3OjeR?= NV7PelZjW3WycILld5Nqd25ib3[gUHBUNXO2aX31cIF1\WRiQ1;YNkBuWk6DIHX4dJJme3Orb36gbY4hdW:3c3WgRnYzKGOnbHzzJIF1KDJyIIXN MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF{NUK3NEc,OTdzMkWyO|A9N2F-
RAW 264.7 NH\GUJZHfW6ldHnvckBie3OjeR?= NXLlTVNDUW6qaXLpeIlwdiCxZjDMVHMh[W6mIGTOSk1idHCqYT3hZ5RqfmG2ZXSgUm8heHKxZIXjeIlwdiCrbjDteZJqdmViUlHXJFI3PC55IHPlcIx{NCCLQ{WwQVI{NjYQvF2u NE\1dHY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{K1N|g1PCd-MUeyOVM5PDR:L3G+
Hepa 1c1c7c1 NVTiU2FUTnWwY4Tpc44h[XO|YYm= M2XnZVEzNjVidV2= MkjWNlQhcHK| M4HTc2lv\HWldHnvckBw\iCzdXnuc45mKHKnZIXjeIF{\SCrbjDtc5V{\SCvdYThcpQhUGWyYTCxZ|FkP2NzIHPlcIx{KGG2IEGyMlUhfU1iYX\0[ZIhOjRiaILzJJJmdGG2aY\lJJRwKGOxboTyc4wtKEGldHn2bZR6RTFwMUdOwG0v NV7mUnh4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezNVY6OThpPkG3N|E3QTF6PD;hQi=>
Hepa 1c1c7 MVXGeY5kfGmxbjDhd5NigQ>? MkC3NVIvPSC3TR?= M3XObVI1KGi{cx?= NGHpVopKdmS3Y4Tpc44hd2ZicYXpco9v\SC{ZXT1Z5Rie2ViYXP0bZZqfHliaX6gcY92e2ViSHXwZUAy[zGlNzDj[YxteyCjdDCxNk42KHWPIHHmeIVzKDJ2IHjyd{Bz\WyjdHn2[UB1dyClb370do9tNCCDY4Tpeol1gT1zLkK0{txONg>? NHTKb2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{OxOlkyQCd-MUezNVY6OTh:L3G+
MC3T3-E1 MUTGeY5kfGmxbjDhd5NigQ>? MXGxJJVO M4TvOXJmfmW{c3HsJI9nKGmwY4LlZZNmKGmwIFXSMmRPSSClb33wcIV5KGmwIH3veZNmKE2FM2SzMWUyKGOnbHzzJJRz[W6|ZnXjeIVlKHerdHig[I9ucW6jboStcoVo[XSrdnWgcZV1[W62IGPyZ{BmgHC{ZYPzbY9vKH[nY4TvdkBifCBzIIXNJIJ6KFKWLWDDVi=> NHHaUlg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MDA-MB-231 NV7mWWo6TnWwY4Tpc44h[XO|YYm= MknJVoV3\XK|YXygc4YhcW6mdXP0bY9vKG:oIF\PXG8{[SCjY4Tpeol1gSCrbjDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KHS{YX7z[oVkfGWmIIfpeIghTk:[T{PhMZNx\WOrZnnjJJNu[WyuIHnueIVz\mW{aX7nJHJPSQ>? M{j3cVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MDA-MB-231 MXTGeY5kfGmxbjDhd5NigQ>? M363UWlv\HWldHnvckBw\iCIT2jPN4Eh[WO2aY\peJkhcW5iaIXtZY4hVUSDLV3CMVI{OSClZXzsdy=> NX7sVpo2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MC3T3-E1 MmjZSpVv[3Srb36gZZN{[Xl? NX[5ZZlCOSC3TR?= NUfkSpMyWmW4ZYLzZYwhd2ZiaX7jdoVie2ViaX6gV5JkKGurbnHz[UBxcG:|cHjvdplt[XSrb36gbY4hdW:3c3WgUWM{XDNvRUGgZ4VtdHNiYYSgNUB2VSCrbjDwdoV{\W6lZTDv[kBRWDJ? NVm3PJl1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MC3T3-E1 MVrGeY5kfGmxbjDhd5NigQ>? M4e4[VEhfU1? NWS3ZZFVWmW4ZYLzZYwhd2ZiaX7jdoVie2ViaX6gV5JkKGurbnHz[UBxcG:|cHjvdplt[XSrb36gbY4hdW:3c3WgUWM{XDNvRUGgZ4VtdHNidILhcpNn\WO2ZXSge4l1cCCmb33pcoFvfC2wZXfheIl3\SCvdYThcpQhW3KlIHX4dJJme3Orb36geoVkfG:{IHH0JFEhfU1? NY\se4o6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MC3T3-E1 NGP3dHNHfW6ldHnvckBie3OjeR?= Mke2NUB2VQ>? NInXVFhKdmO{ZXHz[UBqdiCVcnOgb4lv[XOnIIDoc5NxcG:{eXzheIlwdiCrbjDtc5V{\SCPQ{PUN{1GOSClZXzsd{BifCBzIIXN NIK0eIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MC3T3-E1 NV\QNlFOTnWwY4Tpc44h[XO|YYm= NVH4PIJlWmW4ZYLzZYwhd2ZiaX7jdoVie2ViaX6gSG5CNWKrbnTpcoch[WO2aY\peJkhd2ZiRWLhcJBp[SCrbjDueYNt\WG{IHX4eJJi[3S|IH;mJI1wfXOnIF3DN3Q{NUVzIHPlcIx{KGK7IHXs[YN1em:yaH;y[ZRq[yCvb3LpcIl1gSC|aHnmeEBie3OjeYOgbY4heHKnc3XuZ4Uhd2Zic3jvdpQhcW62ZYLm[ZJqdmdiRWKgZYxxcGFvUl7B NHvFRmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MC3T3-E1 NYTV[Xp6TnWwY4Tpc44h[XO|YYm= MW\S[ZZmenOjbDDv[kBqdmO{ZXHz[UBqdiCGTlGtZolv\GmwZzDhZ5Rqfmm2eTDv[kBGWmGucHjhJIlvKG63Y3zlZZIh\Xi2cnHjeJMhd2ZibX;1d4UhVUN|VEOtSVEh[2WubIOgZpkh\WynY4Tyc5Bpd3KndHnjJI1w[mmuaYT5JJNpcW[2IHHzd4F6eyCrbjDwdoV{\W6lZTDv[kBKS0lvMUiyO|gx NUH5UYxSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
T47D MmriRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MlS4NUB2VQ>? M4jsUWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iVES3SEBk\WyuczDheEAyKHWPIHL5JIZtd3diY4n0c41mfHK7 MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
primary osteoblast cells MlWxSpVv[3Srb36gZZN{[Xl? MmXMNUB2VQ>? NF7m[|NT\X[ncoPhcEBw\iCrbnPy[YF{\SCrbjDFVk5FVkFiY3;tdIxmgCCrbjDyZZQheHKrbXHyfUBwe3Snb3LsZZN1KGOnbHzzJJRz[W6|ZnXjeIVlKHerdHig[I9ucW6jboStcoVo[XSrdnWgcZV1[W62IGPyZ{BmgHC{ZYPzbY9vKH[nY4TvdkBifCBzIIXNJIJ6KFKWLWDDVi=> MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MC3T3-E1 MkizSpVv[3Srb36gZZN{[Xl? MYCxJJVO NVnue3pYWmW4ZYLzZYwhd2ZiaX7jdoVie2ViaX6gdGJOWDJiZYjwdoV{e2mxbjDpckBud3W|ZTDNR|NVOy2HMTDj[YxteyCjdDCxJJVOKGK7IHz1Z4ln\XKjc3WgcYV1cG:mIHnuJJBz\XOnbnPlJI9nKFCSMh?= M2HvRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MC3T3-E1 NWj3N4VQTnWwY4Tpc44h[XO|YYm= Mm\mNUB2VQ>? NFztZWJKdmS3Y4Tpc44hd2ZiRWKgZZN{d2OrYYTpc44hf2m2aDDCUXAzKHC{b33veIVzKGmwIH3veZNmKE2FM2SzMWUyKGOnbHzzJIF1KDFidV2gZpkhS2irUDDhd5NigQ>? NHPMeJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MC3T3-E1 M1P5[mZ2dmO2aX;uJIF{e2G7 MWOxJJVO NGC4fpdT\X[ncoPhcEBw\iCrbnT1Z5Rqd25iaX6gcpVkdGWjcjD0doFve2yxY3H0bY9vKG:oIFXSZYxxcGFiaX6gcY92e2ViTVOzWFMuTTFiY3XscJMhfHKjboPm[YN1\WRid3n0bEBld22rbnHueE1v\WejdHn2[UBufXSjboSgV5JkKGW6cILld5Nqd25idnXjeI9zKGG2IEGgeW0> NXLWdWJ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MG63 NXPRPHY6TnWwY4Tpc44h[XO|YYm= MkLsNE4yKHSxIEGwJJVO NVHQfZp[OjRiaIK= MWjJcoNz\WG|ZTDpckBxem:uaX\ldoF1cW:wIH;mJIh2dWGwIF3HOlMh[2WubIOgZZQhOC5zIITvJFExKHWPIHHmeIVzKDJ2IHjyJIJ6KG[ub4egZ5l1d22ndIL5 M3nLelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MC3T3-E1 M1T1N2Z2dmO2aX;uJIF{e2G7 NWjkRVRWUW6lcnXhd4UhcW5iRF7BMYJqdmSrbnegZYN1cX[rdImgc4YhTVKjbIDoZUBqdiCwdXPs[YFzKGW6dILhZ5R{KG:oIH3veZNmKE2FM2SzMWUyKGOnbHzzJIJ6KGWuZXP0do9xcG:{ZYTpZ{Bud2KrbHn0fUB{cGmodDDhd5NigXN? M1K2XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MCF7 MWXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NUXTUlViOSC3TR?= NVLTT|BpSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIF1KDFidV2gZpkh\myxdzDjfZRwdWW2com= MkjLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
MC3T3-E1 MYrGeY5kfGmxbjDhd5NigQ>? NYHUPWRUOSC3TR?= NVTLb4ZtWmW4ZYLzZYwhd2ZiaX7jdoVie2ViaX6gdGJOWDJiZYjwdoV{e2mxbjDpckBud3W|ZTDNR|NVOy2HMTDj[YxteyC2cnHud4Zm[3SnZDD3bZRpKGSxbXnuZY51NW6nZ3H0bZZmKG23dHHueEBUemNiZYjwdoV{e2mxbjD2[YN1d3JiYYSgNUB2VSCkeTDseYNq\mW{YYPlJI1mfGixZB?= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MC3T3-E1 MliwSpVv[3Srb36gZZN{[Xl? MXOwMlEhfG9iMUCgeW0> NXz6WoliPyCmYYnz MmXCTY5kemWjc3WgbY4hSUySIHHjeIl3cXS7IHnuJI1wfXOnIF3DN3Q{NUVzIHPlcIx{KGG2IECuNUB1dyBzMDD1UUBi\nSncjC3JIRigXN? NXHYXGhwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
primary osteoblast cells NW\zcZBMTnWwY4Tpc44h[XO|YYm= MVewMlEhfG9iMUCgeW0> NFPs[4Y4KGSjeYO= NFzmOmdKdmO{ZXHz[UBqdiCDTGCgZYN1cX[rdImgbY4hemG2IIDybY1ienlib4P0[Y9jdGG|dDDj[YxteyCjdDCwMlEhfG9iMUCgeW0h[W[2ZYKgO{Bl[Xm| MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MC3T3-E1 MnTKSpVv[3Srb36gZZN{[Xl? MlTHNUB2VQ>? Mlj5O|IhcHK| Mn\xTY5kemWjc3WgbY4hd3O2ZX;jZYxkcW5ibWLORUBt\X[nbIOgbY4hdW:3c3WgUWM{XDNvRUGgZ4VtdHNiYYSgNUB2VSCjZoTldkA4OiCqcoOgZpkhWlRvUFPS MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MG63 MYfGeY5kfGmxbjDhd5NigQ>? NXrtVZN2OSC3TR?= NXzsb29NPzJiaILz NFvkb|hKdmO{ZXHz[UBqdiCxc4Tlc4NidGOrbjDtVm5CKGyndnXsd{BqdiCqdX3hckBOTzZ|IHPlcIx{KGG2IEGgeW0h[W[2ZYKgO|IhcHK|IHL5JHJVNVCFUh?= MnTYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
primary osteoblast cells MWXGeY5kfGmxbjDhd5NigQ>? MkK2NUB2VQ>? NFLRVZU4OiCqcoO= MYjJcoNz\WG|ZTDpckBwe3Snb3PhcINqdiCvUl7BJIxmfmWuczDpckBz[XRicILpcYFzgSCxc4Tlc4Jt[XO2IHPlcIx{KGG2IEGgeW0h[W[2ZYKgO|IhcHK|IHL5JHJVNVCFUh?= MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MC3T3-E1 Mk\XSpVv[3Srb36gZZN{[Xl? M2q5SlEhfU1? NFT0W2Q4OiCqcoO= MXvJcoNz\WG|ZTDpckBwe3Snb3PhcINqdiCuZY\lcJMhcW5ibX;1d4UhVUN|VEOtSVEh[2WubIOgZZQhOSC3TTDh[pRmeiB5MjDodpMh[nliRVzJV2E> NXvjUHA3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MG63 NWfNRW1JTnWwY4Tpc44h[XO|YYm= M3rXRlEhfU1? MVy3NkBpenN? NXzLNmVMUW6lcnXhd4UhcW5ib4P0[Y9k[WylaX6gcIV3\Wy|IHnuJIh2dWGwIF3HOlMh[2WubIOgZZQhOSC3TTDh[pRmeiB5MjDodpMh[nliRVzJV2E> NYnNN286RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
primary osteoblast cells M2PSdGZ2dmO2aX;uJIF{e2G7 NYHJOFlbOSC3TR?= NHSwSZI4OiCqcoO= MlTKTY5kemWjc3WgbY4hd3O2ZX;jZYxkcW5ibHX2[Yx{KGmwIILheEBxemmvYYL5JI9{fGWxYnzhd5Qh[2WubIOgZZQhOSC3TTDh[pRmeiB5MjDodpMh[nliRVzJV2E> NIrKSVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MC3T3-E1 MWrGeY5kfGmxbjDhd5NigQ>? NYjTUHBnOSC3TR?= MlzaO|IhcHK| NYXqVW5[UW6lcnXhd4UhcW5ib4P0[Y9xd262aX6gcXJPSSCuZY\lcJMhcW5ibX;1d4UhVUN|VEOtSVEh[2WubIOgZZQhOSC3TTDh[pRmeiB5MjDodpMh[nliUmStVGNT NWrCZWw6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MG63 NHSxO5lHfW6ldHnvckBie3OjeR?= MmXHNUB2VQ>? M1fhbFczKGi{cx?= MlLOTY5kemWjc3WgbY4hd3O2ZX;wc451cW5ibWLORUBt\X[nbIOgbY4hcHWvYX6gUWc3OyClZXzsd{BifCBzIIXNJIFnfGW{IEeyJIhzeyCkeTDSWE1RS1J? NUTOSIxZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
primary osteoblast cells NX;ZS2dsTnWwY4Tpc44h[XO|YYm= NH7TRpEyKHWP NETJR4Q4OiCqcoO= MonDTY5kemWjc3WgbY4hd3O2ZX;wc451cW5ibWLORUBt\X[nbIOgbY4hemG2IIDybY1ienlib4P0[Y9jdGG|dDDj[YxteyCjdDCxJJVOKGGodHXyJFczKGi{czDifUBTXC2SQ2K= M3y1T|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MC3T3-E1 NWjjfHB1TnWwY4Tpc44h[XO|YYm= M2nyfVEhfU1? NFPiOWQ4OiCqcoO= NWLVS3RRUW6lcnXhd4UhcW5ib4P0[Y9xd262aX6gcIV3\Wy|IHnuJI1wfXOnIF3DN3Q{NUVzIHPlcIx{KGG2IEGgeW0h[W[2ZYKgO|IhcHK|IHL5JGVNUVOD M2e2XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MG63 MoDQSpVv[3Srb36gZZN{[Xl? NG\HcIoyKHWP NIjFdIg4OiCqcoO= M2H2bGlv[3KnYYPlJIlvKG:|dHXvdI9vfGmwIHzleoVteyCrbjDoeY1idiCPR{[zJINmdGy|IHH0JFEhfU1iYX\0[ZIhPzJiaILzJIJ6KEWOSWPB NYnXbnU{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
primary osteoblast cells M2jOXWZ2dmO2aX;uJIF{e2G7 MnLTNUB2VQ>? NUHQWllkPzJiaILz NUDZTo04UW6lcnXhd4UhcW5ib4P0[Y9xd262aX6gcIV3\Wy|IHnuJJJifCCycnntZZJ6KG:|dHXvZoxie3RiY3XscJMh[XRiMTD1UUBi\nSncjC3NkBpenNiYomgSWxKW0F? NHzsb5k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MC3T3-E1 M1;oRmZ2dmO2aX;uJIF{e2G7 M4\4UlI1KGi{cx?= MnfSTY5pcWKrdHnvckBw\iCWTl[tZYxxcGFvaX7keYNm\CCjcH;weI9{cXNiaX6gcY92e2ViTVOzWFMuTTFiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JIZtd3diY4n0c41mfHK7 M4LqUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
primary osteoblast cells NYH0fY13TnWwY4Tpc44h[XO|YYm= MkP1NlQhcHK| M3vuZWlvcGmkaYTpc44hd2ZiZYTvdI9{cWSnLXnu[JVk\WRiYYDvdJRwe2m|IHnuJJJifCCycnntZZJ6KG:|dHXvZoxie3RiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JIZtd3diY4n0c41mfHK7 NGG1[HU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
primary osteoblast cells NV;ISnR1TnWwY4Tpc44h[XO|YYm= MXGyOEBpenN? MkXxTY5pcWKrdHnvckBw\iCWTl[tZYxxcGFvaX7keYNm\CCjcH;weI9{cXNiaX6gdoF1KHC{aX3hdpkhd3O2ZX;icIF{fCClZXzsd{Bi\nSncjCyOEBpenNiYomg[oxwfyCleYTvcYV1enl? NGnnUIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MCF7 MVnGeY5kfGmxbjDhd5NigQ>? NIXDOWwyKHWP NF3vPYFT\WS3Y4Tpc44hcW5iYX7jbI9z[WenLXnu[IVx\W6mZX70JINwdG:weTDmc5Ju[XSrb36gc4YhcHWvYX6gUWNHPyClZXzsd{BifCBzIIXNJIJ6KHOxZoSgZYdieiClb3zvcpkh\m:{bXnu[{Bie3OjeR?= MlmwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
T47D NGjkeJVHfW6ldHnvckBie3OjeR?= MkPoNUB2VQ>? NWnFZm5MWmWmdXP0bY9vKGmwIHHuZ4hwemGpZT3pcoRmeGWwZHXueEBkd2yxbomg[o9zdWG2aX;uJI9nKGi3bXHuJHQ1P0RiY3XscJMh[XRiMTD1UUBjgSC|b3\0JIFo[XJiY3;sc456KG[xcn3pcoch[XO|YYm= NWfub3dXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
primary osteoblast cells Mnq1SpVv[3Srb36gZZN{[Xl? MV6xJJVO M{LrNWlv[3KnYYPlJIlvKEKPUEKgcXJPSSCuZY\lcJMhcW5icnH0JJBzcW2jcomgc5N1\W:kbHHzeEBk\WyuczDheEAyKHWPIHL5JHJVNVCFUjDt[ZRpd2R? M4PO[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
primary osteoblast cells M3\MN2Z2dmO2aX;uJIF{e2G7 MVyxJJVO NX;pUXNPWmW4ZYLzZYwhd2ZiaX7jdoVie2ViaX6gSXIvTE6DIHPvcZBt\XhiaX6gdoF1KHC{aX3hdpkhd3O2ZX;icIF{fCClZXzsd{BifCBzIIXNJIJ6KFKWLWDDVkBqdiCycnXz[Y5k\SCxZjDQVFI> MnvWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
MC3T3-E1 MYDGeY5kfGmxbjDhd5NigQ>? MX[xJJVO NUnhVGdMPCCqcoO= NVvoepo6UW6lcnXhd4UhcW5iQl3QNkBmgHC{ZYPzbY9vKGmwIH3veZNmKE2FM2SzMWUyKGOnbHzzJIV5eHKnc4PpcocheEKPUEKgcZV1[W62IHH0JFEhfU1iYX\0[ZIhPCCqcoOgZpkhdHWlaX\ldoF{\SCvZYToc4QhemWuYYTpeoUhfG9iY3;ueJJwdA>? MmLrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
MC3T3-E1 NF76PYJHfW6ldHnvckBie3OjeR?= M4jJNlEhfU1? NUX2dGp6PCCqcoO= MUfJcoNz\WG|ZTDpckBDVVB{IHX4dJJme3Orb36gbY4hdW:3c3WgUWM{XDNvRUGgZ4VtdHNiZYjwdoV{e2mwZzDwRm1ROmSnbIThNUBufXSjboSgZZQhOSC3TTDh[pRmeiB2IHjyd{BjgSCudXPp[oVz[XOnIH3leIhw\CC{ZXzheIl3\SC2bzDjc451em:u M4nQfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MC3T3-E1 Mn\mSpVv[3Srb36gZZN{[Xl? NX\DdWVrOSC3TR?= NYjhbYozWmW4ZYLzZYwhd2ZiaX7keYN1cW:wIH;mJGJOWDJiZYjwdoV{e2mxbjDpckBud3W|ZTDNR|NVOy2HMTDj[YxteyCjdDCxJJVOKGK7IGfld5Rmem5iYnzveEBqdiCycnXz[Y5k\SCxZjDJR2kuOTh{N{iw MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MC3T3-E1 NWXP[m9JTnWwY4Tpc44h[XO|YYm= NUnqXo9DOSC3TR?= Mn:yVoV3\XK|YXygc4YhcW6mdXP0bY9vKG:oIFLNVFIh\XiycnXzd4lwdiCrbjDtc5V{\SCPQ{PUN{1GOSClZXzsd{BifCBzIIXNJIJ6KFenc4Tldo4h[myxdDDpckBxemW|ZX7j[UBw\iC|aH;yeEBqdnSncn\ldolv\yCHUnHsdIhiNVKQQR?= M2i2XFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MC3T3-E1 M1jQUmZ2dmO2aX;uJIF{e2G7 M4PBbFEhfU1? NF7yT|VT\X[ncoPhcEBw\iCrbnT1Z5Rqd25iaX6gcpVkdGWjcjD0doFve2yxY3H0bY9vKG:oIFXSZYxxcGFiaX6gcY92e2ViTVOzWFMuTTFiY3XscJMh[XRiMTD1UUBqdiCycnXz[Y5k\SCxZjDQVFI> MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MC3T3-E1 NVPCTphKTnWwY4Tpc44h[XO|YYm= NFnV[5MyKHWP NFvJcYtT\X[ncoPhcEBw\iCrbnT1Z5Rqd25iaX6gRm1ROiCneIDy[ZN{cW:wIHnuJI1wfXOnIF3DN3Q{NUVzIHPlcIx{KHS{YX7z[oVkfGWmIIfpeIgh\G:vaX7hcpQudmWpYYTpeoUhdXW2YX70JHNz[yCneIDy[ZN{cW:wII\lZ5RweiCjdDCxJJVOKGK7IGfld5Rmem5iYnzveC=> M1PiTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MC3T3-E1 Mn3wSpVv[3Srb36gZZN{[Xl? NVXxNpNjOSC3TR?= MnXrVoV3\XK|YXygc4YhcW6mdXP0bY9vKGmwIFLNVFIh\XiycnXzd4lwdiCrbjDtc5V{\SCPQ{PUN{1GOSClZXzsd{BifCBzIIXNJIJ6KFenc4Tldo4h[myxdDDpckBxemW|ZX7j[UBw\iCSUEK= M33PRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MC3T3-E1 MoO5SpVv[3Srb36gZZN{[Xl? MkjnNUB2VQ>? M{P1dXJmfmW{c3HsJI9nKGmwY4LlZZNmKGmwIFXSMmRPSSClb33wcIV5KGmwIH3veZNmKE2FM2SzMWUyKGOnbHzzJIF1KDFidV2gZpkhWlRvUFPSJIlvKHC{ZYPlcoNmKG:oIGDQNi=> NEO1b5A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
primary osteoblast cells MlTDSpVv[3Srb36gZZN{[Xl? M3jPS|EhfU1? NUj1UWczWmW4ZYLzZYwhd2ZiaX7jdoVie2ViaX6gdGJOWDJiZYjwdoV{e2mxbjDpckBz[XRicILpcYFzgSCxc4Tlc4Jt[XO2IHPlcIx{KHS{YX7z[oVkfGWmIIfpeIgh\G:vaX7hcpQudmWpYYTpeoUhdXW2YX70JHNz[yCneIDy[ZN{cW:wII\lZ5RweiCjdDCxJJVOKGK7IHz1Z4ln\XKjc3WgcYV1cG:m MkLoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
primary osteoblast cells M4TqVGZ2dmO2aX;uJIF{e2G7 MVSxJJVO MVvS[ZZmenOjbDDv[kBqdmO{ZXHz[UBqdiCyQl3QNkBmgHC{ZYPzbY9vKGmwIILheEBxemmvYYL5JI9{fGWxYnzhd5Qh[2WubIOgZZQhOSC3TTDifUBtfWOrZnXyZZNmKG2ndHjv[EBqdiCycnXz[Y5k\SCxZjDQVFI> MmLmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
MC3T3-E1 MmHmSpVv[3Srb36gZZN{[Xl? NYPJTWdyWmW4ZYLzZYwhd2ZiaX7jdoVie2ViaX6gSG5CNWKrbnTpcoch[WO2aY\peJkhd2ZiRWLhcJBp[SCrbjDueYNt\WG{IHX4eJJi[3S|IH;mJI1wfXOnIF3DN3Q{NUVzIHPlcIx{KHS{YX7z[oVkfGWmIIfpeIgh\G:vaX7hcpQudmWpYYTpeoUhdXW2YX70JHNz[yCneIDy[ZN{cW:wII\lZ5RweiCkeTDlcIVkfHKxcHjvdoV1cWNibX;ibYxqfHlic3jp[pQh[XO|YYnz MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MC3T3-E1 MWXGeY5kfGmxbjDhd5NigQ>? MXrS[ZZmenOjbDDv[kBqdmO{ZXHz[UBqdiCGTlGtZolv\GmwZzDhZ5Rqfmm2eTDv[kBGWmGucHjhJIlvKG63Y3zlZZIh\Xi2cnHjeJMhd2ZibX;1d4UhVUN|VEOtSVEh[2WubIOgZpkh\WynY4Tyc5Bpd3KndHnjJI1w[mmuaYT5JJNpcW[2IHHzd4F6eyCrbjDwdoV{\W6lZTDv[kBRWDJ? NXm5PVJbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MC3T3-E1 M4PCdWZ2dmO2aX;uJIF{e2G7 MVexJJVO M1;6XHJmfmW{c3HsJI9nKGmwY4LlZZNmKGmwIFLNVFIh\XiycnXzd4lwdiCrbjDtc5V{\SCPQ{PUN{1GOSClZXzsd{BmgHC{ZYPzbY5oKHOvYXzsJIlvfGW{ZnXybY5oKFKQQTDmc5IhTk:[QUGgdJJwfGWrbjDheEAyKHWPIHL5JHdme3Sncn6gZoxwfA>? MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MC3T3-E1 NWfJNJBpTnWwY4Tpc44h[XO|YYm= MWCxJJVO M1[5T3JmfmW{c3HsJI9nKGmwY4LlZZNmKGmwIIDCUXAzKGW6cILld5Nqd25iaX6gcY92e2ViTVOzWFMuTTFiY3XscJMh\XiycnXzd4lv\yC|bXHscEBqdnSncn\ldolv\yCUTlGg[o9zKE[RWFGxJJBzd3SnaX6gZZQhOSC3TTDifUBtfWOrZnXyZZNmKG2ndHjv[C=> MoTJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
MC3T3-E1 NWL1PXFWTnWwY4Tpc44h[XO|YYm= MmTJNUB2VQ>? NXTxOmRiWmW4ZYLzZYwhd2ZiaX7jdoVie2ViaX6gSXIvTE6DIHPvcZBt\XhiaX6gcY92e2ViTVOzWFMuTTFiY3XscJMh\XiycnXzd4lv\yC|bXHscEBqdnSncn\ldolv\yCUTlGg[o9zKE[RWFGxJJBzd3SnaX6gZZQhOSC3TTDifUBTXC2SQ2K= NYjxWYNiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MDA-MB-231 NVnrfohkTnWwY4Tpc44h[XO|YYm= M334[nJmfmW{c3HsJI9nKGmwaHnibZRqd25iaX6gZY5kcG:{YXflMYlv\GWyZX7k[Y51KGOxbH;ufUBnd3KvYYTpc44hd2ZiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{B1emGwc3\lZ5Rm\CC5aYToJGZQYE9|YT3zdIVkcW[rYzDzcYFtdCCrboTldoZmemmwZzDSUmEh[nlic3;meEBi\2G{IHPvcI9vgSCob4LtbY5oKGG|c3H5 MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MDA-MB-231 Mo\TSpVv[3Srb36gZZN{[Xl? NF;5RlNKdmirYnn0bY9vKG:oIFnHSk1qdmS3Y3XkJGZQYE9|YTDwbI9{eGixconsZZRqd25iYYSgWIhzOzJicnXzbYR2\SCrbjDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGK7IGfld5Rmem5iYnzveC=> M1S4PVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MDA-MB-231 MV\GeY5kfGmxbjDhd5NigQ>? MVrJcohq[mm2aX;uJI9nKEmJRj3pcoR2[2WmIFHreEBxcG:|cHjvdplt[XSrb36gbY4hcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDifUBY\XO2ZYLuJIJtd3R? MlfnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
MDA-MB-231 NY\yO3FrTnWwY4Tpc44h[XO|YYm= MVfS[ZZmenOjbDDv[kBqdmS3Y4Tpc44hd2ZiQXv0JIlv[WO2aY\heIlwdiCrbjDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KHS{YX7z[oVkfGWmIIfpeIgh[2:wc4TpeJV1cX[nbImgZYN1cX[jdHXkJGFsfCCvdYThcpQ> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MDA-MB-231 Mmf2SpVv[3Srb36gZZN{[Xl? NGSy[XBT\X[ncoPhcEBw\iCrbnT1Z5Rqd25ib3[gSm9ZVzOjIH71Z4xm[XJiYXPjeY12dGG2aX;uJIlvKGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgeJJidnOoZXP0[YQhf2m2aDDjc45{fGm2dYTpeoVtgSCjY4TpeoF1\WRiQXv0JI12fGGwdB?= NULiNmVZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MDA-MB-231 Mny2SpVv[3Srb36gZZN{[Xl? NGPnSXpT\X[ncoPhcEBw\iCrbnT1Z5Rqd25ib3[gSm9ZVzOjIHHjeIl3cXS7IHnuJIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNidILhcpNn\WO2ZXSge4l1cCClb37zeIl1fXSrdnXsfUBi[3SrdnH0[YQhSWu2IH31eIFvfA>? NFTvcmY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MDA-MB-231 MVjGeY5kfGmxbjDhd5NigQ>? NV\NWZBMTW6qYX7j[Y1mdnRib3[gRYt1KGmwYXP0bZZifGmxbjDpckBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IITyZY5{\mWldHXkJJdqfGhiZH;tbY5idnRibnXnZZRqfmViQXv0JI12fGGwdB?= M{PyXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MDA-MB-231 M33MemZ2dmO2aX;uJIF{e2G7 NHP3doNGdmijbnPlcYVvfCCxZjDGU3hQO2FiboXjcIVieiCjY3P1cZVt[XSrb36gbY4hcHWvYX6gUWRCNU2ELUKzNUBk\WyuczD0doFve2[nY4Tl[EB4cXSqIHTvcYlv[W62IH7l[4F1cX[nIFHreEBufXSjboS= Ml\VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
MDA-MB-231 M1LNbmZ2dmO2aX;uJIF{e2G7 NX\McWFpTW6qYX7j[Y1mdnRib3[gSm9ZVzOjIHHjeIl3cXS7IHnuJIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNidILhcpNn\WO2ZXSge4l1cCCmb33pcoFvfCCwZXfheIl3\SCDa4SgcZV1[W62 NI[weWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MDA-MB-231 NHLnd5hHfW6ldHnvckBie3OjeR?= NGDsb|MyOCCvZz;r[y=> NX;tVZpDOiCmYYnz NInrdYlF\WO{ZXHz[UBqdiCDa4SgZYN1cX[jdHnvckBqdiCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIV5eHKnc4PpcochTk:[T{PhMZNx\WOrZnnjJJNu[WyuIHnueIVz\mW{aX7nJHJPSSC6ZX7v[5Ji\nSnZDDpckBvfWSnIH3pZ4Uh[XRiMUCgcYcwc2diZn;yJFIh\GG7cx?= MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MDA-MB-231 MYrGeY5kfGmxbjDhd5NigQ>? MVOxNEBu\y:tZx?= MmDFNkBl[Xm| MlHoTY5lfWO2aX;uJI9nKE[RWF:zZUBvfWOuZXHyJIFk[3WvdXzheIlwdiCrbjDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KE[RWF:zZU1{eGWlaX\pZ{B{dWGubDDpcpRmem[ncnnu[{BTVkFieHXuc4dz[W[2ZXSgbY4hdnWmZTDtbYNmKGG2IEGwJI1oN2upIH\vdkAzKGSjeYO= NYD1TXU{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MDA-MB-231 M17Ub2FvfGmycn;sbYZmemG2aY\lJIF{e2G7 MoL4NUB2VQ>? NFzBdYtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYYSgNUB2VSCkeTDmcI94KGO7dH;t[ZRzgQ>? NYjCOmxpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MDA-MB-231 NES2VpBHfW6ldHnvckBie3OjeR?= NXL6SYJtOSC3TR?= MVvS[YR2[3Srb36gbY4h[W6laH;yZYdmNWmwZHXw[Y5l\W62IHPvcI9vgSCob4LtZZRqd25ib3[gbJVu[W5iTVTBMW1DNTJ|MTDj[YxteyCjdDCxJJVOKGK7IIPv[pQh[WejcjDjc4xwdnliZn;ycYlv\yCjc4PhfS=> NUCzSol4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MC3T3-E1 MVLGeY5kfGmxbjDhd5NigQ>? MnnhNUB2VQ>? NXTFfI85PDhiaILz MnnTTY5pcWKrdHnvckBw\iCrbnT1Z5Rqd25ib3[gRWxRKGGldHn2bZR6KGmwIH3veZNmKE2FM2SzMWUyKGOnbHzzJIF1KDFidV2gZYZ1\XJiNEigbJJ{KGmwIIDy[ZNmdmOnIH;mJGJOWDJvc4DlZ4lncWNibnX1eJJidGm8aX7nJIFvfGmkb3T5 NX3VbpFnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
primary osteoblast cells NEfndIpHfW6ldHnvckBie3OjeR?= Mnv4NUB2VQ>? M{e3RlQ5KGi{cx?= MVfJcohq[mm2aX;uJI9nKGmwZIXjeIlwdiCxZjDBUHAh[WO2aY\peJkhcW5icnH0JJBzcW2jcomgc5N1\W:kbHHzeEBk\WyuczDheEAyKHWPIHHmeIVzKDR6IHjyd{BqdiCycnXz[Y5k\SCxZjDCUXAzNXOyZXPp[olkKG6ndYTyZYxqgmmwZzDhcpRq[m:meR?= Mom3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
MC3T3-E1 NFTJe|NHfW6ldHnvckBie3OjeR?= MknyNUB2VQ>? NYSxUFl6PDhiaILz NX;rTZBzUW6qaXLpeIlwdiCxZjDpcoR2[3Srb36gc4YhSUySIHHjeIl3cXS7IHnuJI1wfXOnIF3DN3Q{NUVzIHPlcIx{KGG2IEGgeW0h[W[2ZYKgOFghcHK|IHnuJJBz\XOnbnPlJI9nKG6xZ3fpci=> M4WwR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
primary osteoblast cells NIr6Z4VHfW6ldHnvckBie3OjeR?= MV[xJJVO NIXtcFM1QCCqcoO= M2DDfmlvcGmkaYTpc44hd2ZiaX7keYN1cW:wIH;mJGFNWCCjY4Tpeol1gSCrbjDyZZQheHKrbXHyfUBwe3Snb3LsZZN1KGOnbHzzJIF1KDFidV2gZYZ1\XJiNEigbJJ{KGmwIIDy[ZNmdmOnIH;mJI5w\2erbh?= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
primary osteoblast cells NHfKTpRCdnSrYYDvdJRwfGmlIHHjeIl3cXS7IHHzd4F6 NGLL[VYyKHWP NGHCV|E1QCCqcoO= MnjxTY5pcWKrdHnvckBw\iCjboTpZZBweHSxdHnjJIFkfGm4aYT5JIlvKHKjdDDwdolu[XK7IH;zeIVw[myjc4SgZ4VtdHNiYYSgNUB2VSCjZoTldkA1QCCqcoOgbY4heHKnc3XuZ4Uhd2ZiQl3QNk1{eGWlaX\pZ{Bv\XW2cnHsbZpqdmdiYX70bYJw\Hl? MmDTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
primary osteoblast cells MlPYRY51cWGyb4D0c5Rq[yCjY4Tpeol1gSCjc4PhfS=> MU[xJJVO MnjMOFghcHK| NFvIO3hKdmirYnn0bY9vKG:oIHHueIlieG:ydH;0bYMh[WO2aY\peJkhcW5icnH0JJBzcW2jcomgc5N1\W:kbHHzeEBk\WyuczDheEAyKHWPIHHmeIVzKDR6IHjyd{BqdiCycnXz[Y5k\SCxZjDuc4docW5? NFTGeWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MC3T3-E1 NW\PO3l1TnWwY4Tpc44h[XO|YYm= MnLLNE4yKHSxIEGwJJVO Mn7INlQhcHJ? M4\2d2lv[3KnYYPlJIlvKHC{b3zp[oVz[XSrb36gc4YhdW:3c3WgUWM{XDNvRUGgZ4VtdHNiYYPz[ZN{\WRiYYOg[pJi[3Srb36gc4Yh[2WubIOgbY4hWyCyaHHz[UBifCByLkGgeI8hOTBidV2gZYZ1\XJiMkSgbJIh[nliZnzve{BkgXSxbXX0dpk> MmHvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
primary osteoblast cells M3vWVWZ2dmO2aX;uJIF{e2G7 MnLsNE4yKHSxIEGwJJVO MmflNlQhcHJ? MkDrTY5kemWjc3WgbY4heHKxbHnm[ZJifGmxbjDv[kBz[XRicILpcYFzgSCxc4Tlc4Jt[XO2IHPlcIx{KGG2IECuNUB1dyBzMDD1UUBi\nSncjCyOEBpeiCkeTDmcI94KGO7dH;t[ZRzgQ>? MnHoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
MG63 M{TpcmZ2dmO2aX;uJIF{e2G7 NFPr[lIxNjFidH:gNVAhfU1? M13VOFch\GG7cx?= MWPJcoNz\WG|ZTDpckBCVFBiYXP0bZZqfHliaX6gbJVu[W5iTVe2N{Bk\WyuczDheEAxNjFidH:gNVAhfU1iYX\0[ZIhPyCmYYnz NX[1O3V5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MC3T3-E1 Moe4SpVv[3Srb36gZZN{[Xl? NHHxeogzPCCqcoO= Mlf3TY5pcWKrdHnvckBw\iCndH;wc5Nq\GVvaX7keYNm\CCjcH;weI9{cXNiaX6gcY92e2ViTVOzWFMuTTFiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JIZtd3diY4n0c41mfHK7 M4e3TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MC3T3-E1 MVHBcpRq[XCxcITveIlkKGGldHn2bZR6KGG|c3H5 M1m1fVEhfU1? NHjPT3A1QCCqcoO= NXLrUpBXUW6qaXLpeIlwdiCxZjDhcpRq[XCxcITveIlkKGGldHn2bZR6KGmwIH3veZNmKE2FM2SzMWUyKGOnbHzzJIF1KDFidV2gZYZ1\XJiNEigbJJ{KGmwIIDy[ZNmdmOnIH;mJGJOWDJvc4DlZ4lncWNibnX1eJJidGm8aX7nJIFvfGmkb3T5 M{CwO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MC3T3-E1 M1TsPWFvfGmjcH;weI91cWNiYXP0bZZqfHliYYPzZZk> MnPZNUB2VQ>? NX;C[lZNPDhiaILz NUT2OJdbUW6qaXLpeIlwdiCxZjDhcpRq[XCxcITveIlkKGGldHn2bZR6KGmwIH3veZNmKE2FM2SzMWUyKGOnbHzzJIF1KDFidV2gZYZ1\XJiNEigbJJ{KGmwIIDy[ZNmdmOnIH;mJI5w\2erbh?= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
RAW264.7 MUfGeY5kfGmxbjDhd5NigQ>? M4DWNmlvcGmkaYTpc44hd2ZicHjh[49kgXSxc3nzJI9nKE[LVFOtZ49vcnWpYYTl[EBGe2OqZYLpZ4hq[SClb3zpJGsyOiCrbjDtc5V{\SCUQWeyOlQvPyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gdoF1cW9ib3[gcpVu[mW{IH;mJIJi[3SncnnhcEBk\WyuczD0c{BxcGGpb3P5eIV{KHW|aX7nJJRzgXCjbjDicJVmKHO2YXnubY5oKGK7IH\sc5ch[3m2b33leJJ6 M4rqelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUO4NVg4Lz5zN{mzPFE5PzxxYU6=
RAW264.7 MU\GeY5kfGmxbjDhd5NigQ>? M{nsN2lvcGmkaYTpc44hd2ZicHjh[49kgXSxc3nzJI9nKE[LVFOtZ49vcnWpYYTl[EBUfGGyaInsc4Nw[2O3czDheZJmfXNiMkC5VEBqdiCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5icnH0bY8hd2ZiboXtZoVzKG:oIHLhZ5RmemmjbDDj[YxteyC2bzDwbIFod2O7dHXzJJV{cW6pIITyfZBidiCkbIXlJJN1[WmwaX7nJIJ6KG[ub4egZ5l1d22ndIL5 M{nsUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUO4NVg4Lz5zN{mzPFE5PzxxYU6=
THP1 NFXDTYNHfW6ldHnvckBie3OjeR?= M3vZUmlvcGmkaYTpc44hd2ZicHjh[49kgXSxc3nzJI9nKE[LVFOtZ49vcnWpYYTl[EBGe2OqZYLpZ4hq[SClb3zpJGsyOiCrbjDoeY1idiCWSGCxJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiC{YYTpc{Bw\iCwdX3i[ZIhd2ZiYnHjeIVzcWGuIHPlcIx{KHSxIIDoZYdw[3m2ZYOgeZNqdmdidIL5dIFvKGKudXWgd5RicW6rbnegZpkh\myxdzDjfZRwdWW2com= MmqxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7M{ixPFcoRjF5OUO4NVg4RC:jPh?=
THP1 MWrGeY5kfGmxbjDhd5NigQ>? MX3Jcohq[mm2aX;uJI9nKHCqYXfvZ5l1d3OrczDv[kBHUVSFLXPvcop2\2G2ZXSgV5RieGi7bH;jc4NkfXNiYYXy[ZV{KDJyOWCgbY4hcHWvYX6gWGhROSClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gdoF1cW9ib3[gcpVu[mW{IH;mJIJi[3SncnnhcEBk\WyuczD0c{BxcGGpb3P5eIV{KHW|aX7nJJRzgXCjbjDicJVmKHO2YXnubY5oKGK7IH\sc5ch[3m2b33leJJ6 MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzl|OEG4O{c,OTd7M{ixPFc9N2F-
THP1 NV:3[Y1vTnWwY4Tpc44h[XO|YYm= M1;xflEhcHJ? NGfPOW1KdmirYnn0bY9vKG:oIF\TUE0yNWmwZIXj[YQhcW6lcnXhd4UhcW5icHjh[49kgXSxc3nzJI9nKE[LVFOtZ49vcnWpYYTl[EBGe2OqZYLpZ4hq[SClb3zpJGsyOiCrbjDoeY1idiCWSGCxJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCwdX3i[ZIhd2ZiY3XscJMheGijZ3;jfZRwe2mwZzDiZYN1\XKrYTDw[ZIh[2WubDDh[pRmeiBzIHjyJIJ6KEOOU12= MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzl|OEG4O{c,OTd7M{ixPFc9N2F-
THP1 NXn5OXlWTnWwY4Tpc44h[XO|YYm= M4P5PFEhcHJ? M3ntbmlvcGmkaYTpc44hd2ZiRmPMMVEucW6mdXPl[EBqdmO{ZXHz[UBqdiCyaHHnc4N6fG:|aYOgc4YhTkmWQz3jc45rfWejdHXkJGV{[2incnnjbIliKGOxbHmgT|EzKGmwIHj1cYFvKFSKUEGgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKG63bXLldkBw\iCyaHHnc4N6fG:|aX7nJIJi[3SncnnhJJRwKHSxdHHsJI52dWKncjDv[kBk\WyuczDh[pRmeiBzIHjyJIJ6KEOOU12= MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzl|OEG4O{c,OTd7M{ixPFc9N2F-
HEK293 NH60UIpHfW6ldHnvckBie3OjeR?= MmG3NUBpeg>? M1eyUmlvcGmkaYTpc44hd2ZiaHXheEBscWyuZXSgSZNkcGW{aXPobYEh[2:uaTDLNVIucW6mdXPl[EBPTi2tYYDwZWIh[WO2aY\heIlwdiCrbjDISWszQTNiY3XscJMh\XiycnXzd4lv\yCWTGKyJJBz\XS{ZXH0[YQh\m:{IEGgbJIh[mWob4LlJFYhcHK|IHjlZZQhc2mubHXkJIJi[3SncnnhcEB{fGmvdXzheIlwdiCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk> NGH6S4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{mzPFE5Pyd-MUe5N|gyQDd:L3G+
HEK293 NUXQRld3TnWwY4Tpc44h[XO|YYm= MYSxJIhz M2HiZmlvcGmkaYTpc44hd2ZiaHXheEBscWyuZXSgV5RieGi7bH;jc4NkfXNiYYXy[ZV{KDJyOWCtbY5lfWOnZDDOSk1s[XCyYVKgZYN1cX[jdHnvckBqdiCKRVuyPVMh[2WubIOg[ZhxemW|c3nu[{BVVFJ{IIDy[ZRz\WG2ZXSg[o9zKDFiaIKgZoVnd3KnIE[gbJJ{KGinYYSgb4ltdGWmIHLhZ5RmemmjbDDzeIlufWyjdHnvckBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYm= NG\iN4w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{mzPFE5Pyd-MUe5N|gyQDd:L3G+
HEK293 NEG0S5lHfW6ldHnvckBie3OjeR?= NISxS2IyODBidV2= M3OzO|EhcHJ? M{XldGlvcGmkaYTpc44hd2ZiaHXheEBscWyuZXSgSZNkcGW{aXPobYEh[2:uaTDLNVIucW6mdXPl[EBvfWOuZXHyJJRz[W6|bH;jZZRqd25ib3[gdFY2KGWuZX3lcpQhd2ZiTl[tb4FxeGGEIHnuJGhGUzJ7MzDj[YxteyCneIDy[ZN{cW6pIGTMVlIh[XRiMUCwJJVOKHC{ZYTy[YF1\WRiZn;yJFEhcHJiYnXmc5JmKDZiaILzJIhm[XRia3nscIVlKGKjY4TldolidCC|dHnteYxifGmxbjDifUBqdW23bn;zeIFqdmmwZzDt[ZRpd2R? MkLIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7M{ixPFcoRjF5OUO4NVg4RC:jPh?=
HEK293 MVvGeY5kfGmxbjDhd5NigQ>? M1TTU|ExOCC3TR?= MX[xJIhz MoW5TY5pcWKrdHnvckBw\iCqZXH0JItqdGynZDDTeIFxcHmub3PvZ4N2eyCjdYLleZMhOjB7UD3pcoR2[2WmIH71Z4xm[XJidILhcpNtd2OjdHnvckBw\iCyNkWg[YxmdWWwdDDv[kBPTi2tYYDwZWIhcW5iSFXLNlk{KGOnbHzzJIV5eHKnc4PpcochXEyUMjDheEAyODBidV2gdJJmfHKnYYTl[EBnd3JiMTDodkBj\W[xcnWgOkBpenNiaHXheEBscWyuZXSgZoFkfGW{aXHsJJN1cW23bHH0bY9vKGK7IHntcZVvd3O2YXnubY5oKG2ndHjv[C=> NWfMRZg4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5N|gyQDdpPkG3PVM5OTh5PD;hQi=>
RAW264.7 MXjGeY5kfGmxbjDhd5NigQ>? M4rWdFEhfG9iMUCwJJVO NFvKWHE3KGi{cx?= M{PWSmlvcGmkaYTpc44hd2ZiaHXheEBscWyuZXSgSZNkcGW{aXPobYEh[2:uaTDLNVIucW6mdXPl[EBVVkZiYXzwbIEheHKxZIXjeIlwdiCrbjDtc5V{\SCUQWeyOlQvPyClZXzsd{BifCBzIITvJFExOCC3TTDh[pRmeiB4IHjyd{BjgSCHTFnTRS=> MmDRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7M{ixPFcoRjF5OUO4NVg4RC:jPh?=
THP1 MYTGeY5kfGmxbjDhd5NigQ>? M1zh[lEhfG9iMUCwJJVO MYO2JIhzew>? MUDJcohq[mm2aX;uJI9nKGinYYSgb4ltdGWmIFXzZ4hmemmlaHnhJINwdGliS{GyMYlv\HWlZXSgWG5HKGGucHjhJJBzd2S3Y4Tpc44hcW5iaIXtZY4hXEiSMTDj[YxteyCjdDCxJJRwKDFyMDD1UUBi\nSncjC2JIhzeyCkeTDFUGlUSQ>? NXK2cVlQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5N|gyQDdpPkG3PVM5OTh5PD;hQi=>
RAW264.7 MmPOSpVv[3Srb36gZZN{[Xl? NE[0fWgyKHSxIEGwNEB2VQ>? NUnOdpBbPiCqcoO= NXzNWlFUUW6qaXLpeIlwdiCxZjDo[YF1KGurbHzl[EBUfGGyaInsc4Nw[2O3czDheZJmfXNiMkC5VE1qdmS3Y3XkJHRPTiCjbIDoZUBxem:mdXP0bY9vKGmwIH3veZNmKFKDV{K2OE44KGOnbHzzJIF1KDFidH:gNVAxKHWPIHHmeIVzKDZiaILzJIJ6KEWOSWPB MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzl|OEG4O{c,OTd7M{ixPFc9N2F-
THP1 NGXmbmFHfW6ldHnvckBie3OjeR?= NVX2doVHOSC2bzCxNFAhfU1? NW\EfnFvPiCqcoO= MV;Jcohq[mm2aX;uJI9nKGinYYSgb4ltdGWmIGP0ZZBpgWyxY3;jZ5V{KGG3cnX1d{AzODmSLXnu[JVk\WRiVF7GJIFteGijIIDyc4R2[3Srb36gbY4hcHWvYX6gWGhROSClZXzsd{BifCBzIITvJFExOCC3TTDh[pRmeiB4IHjyd{BjgSCHTFnTRS=> NIfGVI89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{mzPFE5Pyd-MUe5N|gyQDd:L3G+
THP1 MXLGeY5kfGmxbjDhd5NigQ>? NGL5VGMyODBidV2= NFnST28yKGi{ MXfJcohq[mm2aX;uJI9nKE[VTEGtbY5lfWOnZDD1dJJm\3WuYYTpc44hd2ZiTWPSNUBuWk6DIHX4dJJme3Orb36gbY4hcW5iaIXtZY4hXEiSMTDj[YxteyCjdDCxNFAhfU1iYX\0[ZIhOSCqcjDifUBTXC2SQ2K= NV7rcXVyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5N|gyQDdpPkG3PVM5OTh5PD;hQi=>
THP1 MmS1SpVv[3Srb36gZZN{[Xl? M3rDfFExOCC3TR?= MX2xJIhz MmDJTY5pcWKrdHnvckBw\iCIU1yxMYlv\HWlZXSgeZBz\We3bHH0bY9vKG:oIFPEN|YhdVKQQTDlfJBz\XO|aX;uJIlvKGmwIHj1cYFvKFSKUEGgZ4VtdHNiYYSgNVAxKHWPIHHmeIVzKDFiaIKgZpkhWlRvUFPS MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzl|OEG4O{c,OTd7M{ixPFc9N2F-
THP1 MV7GeY5kfGmxbjDhd5NigQ>? MYGxNFAhfU1? Mo\JNUBpeg>? MVjJcohq[mm2aX;uJI9nKE[VTEGtbY5lfWOnZDD1dJJm\3WuYYTpc44hd2ZiRFOtV2lIViCvUl7BJIV5eHKnc4Ppc44hcW5iaX6gbJVu[W5iVFjQNUBk\WyuczDheEAyODBidV2gZYZ1\XJiMTDodkBjgSCUVD3QR3I> NV7JbJN5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5N|gyQDdpPkG3PVM5OTh5PD;hQi=>
THP1 NYrmRlZUTnWwY4Tpc44h[XO|YYm= MYSxNFAhfU1? NVz6NFNmOSCqch?= MUnJcohq[mm2aX;uJI9nKE[VTEGtbY5lfWOnZDD1dJJm\3WuYYTpc44hd2ZiZHXjeIlvNTFibWLORUBmgHC{ZYPzbY9vKGmwIHnuJIh2dWGwIGTIVFEh[2WubIOgZZQhOTByIIXNJIFnfGW{IEGgbJIh[nliUmStVGNT M2f5S|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUO4NVg4Lz5zN{mzPFE5PzxxYU6=
HEK293 MWjGeY5kfGmxbjDhd5NigQ>? M{\MVVExOCC3TR?= M3z3TGlvcGmkaYTpc44hd2ZiRFOtV2lIViCneIDy[ZN{cW:wIHnuJGhGUzJ7MzDj[YxteyCneIDy[ZN{cW6pIFTDMXNKT05iYYSgNVAxKHWPIHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? NYD1[3NmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5N|gyQDdpPkG3PVM5OTh5PD;hQi=>
U2OS MlXDSpVv[3Srb36gZZN{[Xl? NV\1ZXpVOTByIIXN NHK3d5hC[3SrdnH0bY9vKG:oIGPJVnQyKGmwIHj1cYFvKFV{T2OgZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4heDV|IHTlZYNmfHmuYYTpc44hdGW4ZXygZZQhOTByIIXN M{Tl[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MES2OFA6Lz5zOEC0OlQxQTxxYU6=
293 NIPGeHFHfW6ldHnvckBie3OjeR?= NHfJN|EzPCCqcoO= NF3mXVNKdmirYnn0bY9vKG:oIGTOSoFteGijIHnu[JVk\WRiTl[tb4FxeGGEIHHjeIl3[XSrb36gbY4hcHWvYX6gNlk{KGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEmFNUC9NVYvOc7:TT6= MnHGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh2OEewOVMoRjF6NEi3NFU{RC:jPh?=
cortical microglial MVrGeY5kfGmxbjDhd5NigQ>? NXfwbFNkPDhiaILz NHnBWJVKdmirYnn0bY9vKG:oIFzQV{1{fGmvdXzheIVlKE6RIILlcIVie2ViaX6gdoF1KGOxcoTpZ4FtKG2rY4Lv[4xq[WxiY3XscJMh[XO|ZYPz[YQh[XNibnn0dol1\SCjY3P1cZVt[XSrb36gZYZ1\XJiNEigbJJ{NCCLQ{WwQVEyNjYQvF2u MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDV6OEO0N{c,OTh3OEizOFM9N2F-
HL60 MXXDfZRwfG:6aXPpeJkh[XO|YYm= NYH1[GZ{PDhiaILz MWLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;M{[uN:69VS5? NEDrUIE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUK1NVQzOCd-MUmyOVE1OjB:L3G+
PBL MVfGeY5kfGmxbjDhd5NigQ>? MUWxNk42KHWP NFnVN4xKdmirYnn0bY9vKG:oIHj5[JJw\2WwIIDldo95cWSnLXnu[JVk\WRiRF7BJIRidWGpZTDpckBpfW2jbjDQRmwh[2WubIOgZZQhOTJwNTD1UUBjgSCFb33leEBie3OjeR?= MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTJ5MUezOEc,OTl{N{G3N|Q9N2F-
HL60 MmfqSpVv[3Srb36gZZN{[Xl? MXuxOVAhfU1? NX3kdVhEPDhiaILz Mmn1TY5lfWO2aX;uJI9nKESQQTDmdoFodWWwdHH0bY9vKGmwIHj1cYFvKEiONkCgZ4VtdHNiYYSgNVUxKHWPIHHmeIVzKDR6IHjyd{BjgSCndHjp[Il2dSCkcn;tbYRmKGKjc3XkJIdmdCCnbHXjeJJweGixcnXzbZM> M2LsTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MkexO|M1Lz5zOUK3NVc{PDxxYU6=
DLKP M{P4XGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M{\JXlUh\GG7cx?= M3S2bGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgUXJROS2neIDy[ZN{cW6pIHj1cYFvKESOS2CgZ4VtdHNiYX\0[ZIhPSCmYYnzJIJ6KGGlaXSgdIhwe3CqYYThd4Uh[XO|YYmsJGlEPTB;MUFOwG0v NF;PWGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS4NVQ3Oid-MUm0PFE1PjJ:L3G+
DLKP-A M3u2OmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MVm1JIRigXN? NWG4b4xWSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBRNWeyIHX4dJJme3OrbnegbJVu[W5iRFzLVE1CKGOnbHzzJIFnfGW{IEWg[IF6eyCkeTDhZ4llKHCqb4PwbIF1[XOnIHHzd4F6NCCLQ{WwQVE2|ryPLh?= MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR6MUS2Nkc,OTl2OEG0OlI9N2F-
HT144 NEDyTY5CdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NH73clI2KGSjeYO= MnrtRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKVEG0OEBk\WyuczDh[pRmeiB3IHThfZMh[nliYXPp[EBxcG:|cHjheIF{\SCjc4PhfUwhUUN3ME20NO69VS5? NFz2eIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS4NVQ3Oid-MUm0PFE1PjJ:L3G+
C2C12 M4LqcWZ2dmO2aX;uJIF{e2G7 MVm1NEB2VQ>? NHe0bJcyPiCqcoO= MlPHTY5kemWjc3WgbY4hdWm2b3Poc45lemmjbDDmeY5kfGmxbjDpckBud3W|ZTDDNmMyOiClZXzsd{Bie3Onc4Pl[EBieyCnbnjhcoNmdWWwdDDv[kBucXSxY3jvcoRzcWGuIHTlcpNqfHliYYSgOVAhfU1iYX\0[ZIhOTZiaILzJIJ6KGe{ZXXuJGZOKGS7ZTDzeJJicW6rbnetZoF{\WRiZnz1c5Jme2OnbnPlJIF{e2G7 MnvqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl4NkO0PVgoRjF7Nk[zOFk5RC:jPh?=
3T3L1 M1jtbWFvfGmjZHnwc4dmdmmlIHHzd4F6 NHy0R2c4KGSjeYO= MVfBcpRq[WSrcH;n[Y5q[yCjY4Tpeol1gSCjZ3HpcpN1KG2xdYPlJFNVO0xzIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yh\GmoZnXy[Y51cWG2aX;uJIFnfGW{IEeg[IF6eyCkeTDvbYwuemWmIF:gd5RicW6rbnesJGlEPTB;M{GuOO69VS5? NH21bHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUe1O|g2Oyd-MUm3OVc5PTN:L3G+
SW480 NFfDZoVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M1PD[FQ5KGi{cx?= MXnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFOZNEiwJINmdGy|IHHzd4V{e2WmIHHzJINmdGxidnnhZoltcXS7IIXzbY5oKHC{b4Dp[Il2dSCrb3Tp[IUhe3SjaX7pcoch[W[2ZYKgOFghcHK|LDDJR|UxRTJyzszNMi=> NUPmN|IzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCzPVUxOTlpPkKwN|k2ODF7PD;hQi=>
H460 MmO4RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NF\2dFM4OiCqcoO= MXPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEh2NkCgZ4VtdHNiYX\0[ZIhPzJiaILzMEBKSzVyPUGyMlnPxE1w MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODRyOUeyN{c,OjB2MEm3NlM9N2F-
H460 MXXD[YxtKGO7Y3zlJIF{e2G7 M3n4NlIxKHWP MnTXNlQhcHK| M1LVPWNmdGxiY4njcIUh[XK{ZYP0JIlvKGi3bXHuJGg1PjBiY3XscJMh[XO|ZYPz[YQh[XNiYXPjeY12dGG2aX;uJIF1KEd{L12gdIhie2ViYYSgNlAhfU1iYX\0[ZIhOjRiaILzJIJ6KHC{b4Dp[Il2dSCrb3Tp[IUhe3SjaX7pcocu[mG|ZXSg[oxwfyCleYTvcYV1enl? NGTObFQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MESwPVczOyd-MkC0NFk4OjN:L3G+
HEK293 NYLwe4syTnWwY4Tpc44h[XO|YYm= NVrUbJc1PDhiaILz NUXGWm5pUW6qaXLpeIlwdiCxZjDUUmYu[WyyaHGtbY5lfWOnZDDOSk1s[XCyYVKgZYN1cX[rdImg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDh[pRmeiB2ODDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyPUCuNVc{|ryPLh?= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODV{N{i5NUc,OjB3Mke4PVE9N2F-
RAW 264.7 MmG1SpVv[3Srb36gZZN{[Xl? NIXDTmUzOCCqcoO= NFfwdVJKdmirYnn0bY9vKG:oIHnOU3MudWWmaXH0[YQhdmm2cnnjJI95cWSnIIDyc4R2[3Srb36gbY4hVFCVLYP0bY12dGG2ZXSgcY92e2ViUlHXJFI3PC55IHPlcIx{KHC{ZYTy[YF1\WRiM{CgcYlveyCkZX\vdoUhVFCVIHPoZYxt\W6pZTDt[YF{fXKnZDDh[pRmeiB{MDDodpMh[nliR4Lp[ZN{KHKnYXP0bY9vKG2ndHjv[EBz\WyjdHn2[UB1dyClb370do9tNCCLQ{WwQVE2|ryPLh?= MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODV{N{i5NUc,OjB3Mke4PVE9N2F-
Hepa 1c1c7 MULGeY5kfGmxbjDhd5NigQ>? MoDzTY5pcWKrdHnvckBw\iCzdXnuc45mKHKnZIXjeIF{\SBzIHnuJI1wfXOnIFjldIEhOWNzY{egZ4VtdHNiYYPz[ZN{\WRiYYOg[JJ2\yCuZY\lcEBz\XG3aYLl[EB1dyCmb4XicIUh\W68eX3lJIFkfGm4aYT5JIJ6KE2WVDDhd5NigSxiQ1S9NlHPxE1w NYnTc29TRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC1Nlc5QTFpPkKwOVI4QDlzPD;hQi=>
RAW264.7 NX;rbWFYTnWwY4Tpc44h[XO|YYm= NVvTfmtHOzRidV2= Mn\sNVUhdWmwcx?= M1ey[GlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgbW5QWyCneIDy[ZN{cW:wIHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KGG2IEO0JJVOKHS{ZXH0[YQhOTVibXnud{Bj\W[xcnWgUHBUKGOqYXzs[Y5o\SCvZXHzeZJm\CCjZoTldkAyQCCqcoOgZpkhX2W|dHXyckBjdG:2dHnu[y=> NFf4S|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEWyO|g6OSd-MkC1Nlc5QTF:L3G+
Caco-2 NYjQfoxvS3m2b4TvfIlkcXS7IHHzd4F6 NE\ySpY{KGSjeYO= MXfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDDZYNwNTJiY3XscJMh[W[2ZYKgN{Bl[Xm|IHL5JHs{UF22aIntbYRqdmViaX7jc5Jxd3KjdHnvckBie3OjeTygTWM2OD1{ND6zOe69VS5? MonvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB4MkezO|koRjJyNkK3N|c6RC:jPh?=
HT-29 NIjlU4hEgXSxdH;4bYNqfHliYYPzZZk> Moi4N{Bl[Xm| MoToR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTHQuOjliY3XscJMh[W[2ZYKgN{Bl[Xm|IHL5JHs{UF22aIntbYRqdmViaX7jc5Jxd3KjdHnvckBie3OjeTygTWM2OD12NT6z{txONg>? NHnqcFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{ME[yO|M4QSd-MkC2Nlc{Pzl:L3G+
MDA-MB-231 MnLJRY51cXS3bX;yJIF{e2G7 M3PlNFYh\GG7cx?= Mmr1RY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBMW1DNTJ|MTDj[YxteyCjZoTldkA3KGSjeYOgZpkhW1KEIHHzd4F6NCCLQ{WwQVIxNjYQvF2u NYHZdlM6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC3Nlg{PjlpPkKwO|I5OzZ7PD;hQi=>
CCD-18Co NGS0[ndCdnSraX7mcIFudWG2b4L5JIF{e2G7 M1nkWlEhfU1? M2P3bFE5KGi{cx?= MVrBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJIh2dWGwIFPDSE0yQEOxIHPlcIx{KGG|c3Xzd4VlKGmwaHnibZRqd25ib3[gTWwy[mW2YT3pcoR2[2WmIGDHSVIheHKxZIXjeIlwdiCjdDCxJJVOKGGodHXyJFE5KGi{cx?= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh4NkCzNkc,OjB6Nk[wN|I9N2F-
CCD-18Co MWHBcpRqcW6obHHtcYF1d3K7IHHzd4F6 M1Tyd|IvPSC2bzCyOUB2VQ>? MmLnNVghcHK| MlPSRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBpfW2jbjDDR2QuOTiFbzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEmOMXLleIEucW6mdXPl[EBRT0V{IIDyc4R2[3Srb36gZZQhOi53IITvJFI2KHWPIHHmeIVzKDF6IHjydy=> NGDTb409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEi2OlA{Oid-MkC4OlYxOzJ:L3G+
Caco2 MYrGeY5kfGmxbjDhd5NigQ>? M2nWVFYhcHK| MUfN[ZRi[m:uaXOgd5Ri[mmuaYT5JIlvKGi3bXHuJGNi[29{IHPlcIx{KGGodHXyJFYhcHK|IHL5JGxENU2VLV3TJIFv[Wy7c3nz M33uOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOE[2NFMzLz5{MEi2OlA{OjxxYU6=
HT22 MnraUoV2em:ycn;0[YN1cX[nIHHzd4F6 M4LoTFI1KGi{cx?= NYrZdHpIVmW3cn;wdo91\WO2aY\lJIFkfGm4aYT5JIlvKG2xdYPlJGhVOjJiY3XscJMh[XO|ZYPz[YQh[XNicILveIVkfGmxbjDh[4FqdnO2IHfseZRidWG2ZT3pcoR2[2WmIH;4fZRwe2m|IHHmeIVzKDJ2IHjyd{BjgSCPVGSgZZN{[XluIFXDOVA:PC54NkhOwG0v MmLPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFzMkm5O|goRjJzMUK5PVc5RC:jPh?=
SW480 MlroR5l1d3SxeHnjbZR6KGG|c3H5 NWr2[|ZrOTBidV2= MXuyJIRigXN? NF\6cYJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUXzR6MDDj[YxteyCjdDCxNEB2VSCob4KgNkBl[Xm|IHL5JJZqNWOnbHygZ4VtdCC4aXHibYxqfHliYX7hcJl{cXN? NISzT2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK5NVI{PSd-MkGyPVEzOzV:L3G+
HCT116 NVLIcXg5S3m2b4TvfIlkcXS7IHHzd4F6 Mon3NVAhfU1? M1nyRlIh\GG7cx?= MoHUR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVOTF4IHPlcIx{KGG2IEGwJJVOKG[xcjCyJIRigXNiYomgeoku[2WubDDj[YxtKH[rYXLpcIl1gSCjbnHsfZNqew>? NHryeZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK5NVI{PSd-MkGyPVEzOzV:L3G+
HCT116 NHXOcWhEgXSxdH;4bYNqfHliYYPzZZk> NXX3W2pHOTBidV2= NEC4eYw1KGSjeYO= MVTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[XRiMUCgeW0h\m:{IESg[IF6eyCkeTD2bU1k\WyuIHPlcIwhfmmjYnnsbZR6KGGwYXz5d4l{ MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ7MUKzOUc,OjF{OUGyN|U9N2F-
LS 174T MmXsR5l1d3SxeHnjbZR6KGG|c3H5 NGG3N2syOCC3TR?= NVq0TXh2PCCmYYnz Mnq1R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUHMhOTd2VDDj[YxteyCjdDCxNEB2VSCob4KgOEBl[Xm|IHL5JJZqNWOnbHygZ4VtdCC4aXHibYxqfHliYX7hcJl{cXN? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ7MUKzOUc,OjF{OUGyN|U9N2F-
LS 174T M3;mNmN6fG:2b4jpZ4l1gSCjc4PhfS=> MkXINVAhfU1? MVWyJIRigXN? MVXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMV{AyPzSWIHPlcIx{KGG2IEGwJJVOKG[xcjCyJIRigXNiYomgeoku[2WubDDj[YxtKH[rYXLpcIl1gSCjbnHsfZNqew>? MoTYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{OUGyN|UoRjJzMkmxNlM2RC:jPh?=
LS174T NYX0RWhTTnWwY4Tpc44h[XO|YYm= MX2xNFAhfU1? MlT4N|YhcHK| MVXJcohq[mm2aX;uJI9nKFewdD;i[ZRiNWOjc3XpckBqdiCqdX3hckBNWzF5NGSgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKEurNkegcXJPSSCuZY\lcEBifCBzMECgeW0h[W[2ZYKgN|YhcHK|IHL5JHJVNVCFUjDhcoFtgXOrcx?= M3voNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmxNlM2Lz5{MUK5NVI{PTxxYU6=
LS174T MW\GeY5kfGmxbjDhd5NigQ>? M3;hOFExOCC3TR?= MWmzOkBpenN? NEj6PYZKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPhd4VqdiCrbjDoeY1idiCOU{G3OHQh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHOtUZlkKG2UTlGgcIV3\WxiYYSgNVAxKHWPIHHmeIVzKDN4IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZM> MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ7MUKzOUc,OjF{OUGyN|U9N2F-
LS174T M1r3SGZ2dmO2aX;uJIF{e2G7 NUS5SYdNOTByIIXN NXXBTVM3OzZiaILz MX\Jcohq[mm2aX;uJI9nKFewdD;i[ZRiNWOjc3XpckBqdiCqdX3hckBNWzF5NGSgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKEOGNESgcXJPSSCuZY\lcEBifCBzMECgeW0h[W[2ZYKgN|YhcHK|IHL5JHJVNVCFUjDhcoFtgXOrcx?= NYrabGhxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyPVEzOzVpPkKxNlkyOjN3PD;hQi=>
LS174T NHzyeoNHfW6ldHnvckBie3OjeR?= NGXSZ44yODBidV2= NIXaW5k{PiCqcoO= M{PVZmlvcGmkaYTpc44hd2ZiV370M4JmfGFvY3Hz[YlvKGmwIHj1cYFvKEyVMUe0WEBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iTHfyOUBuWk6DIHzleoVtKGG2IEGwNEB2VSCjZoTldkA{PiCqcoOgZpkhWlRvUFPSJIFv[Wy7c3nz M3:w[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmxNlM2Lz5{MUK5NVI{PTxxYU6=
LS174T MYTGeY5kfGmxbjDhd5NigQ>? MkH0NVAxKHWP MYWzOkBpenN? NE\CeW9KdmirYnn0bY9vKG:oIGfueE9j\XSjLXPhd4VqdiCrbjDoeY1idiCOU{G3OHQh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIIP1dpZqfmmwIH3SUmEhdGW4ZXygZZQhOTByIIXNJIFnfGW{IEO2JIhzeyCkeTDSWE1RS1JiYX7hcJl{cXN? NHXGeYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK5NVI{PSd-MkGyPVEzOzV:L3G+
LS174T NIOyUYpHfW6ldHnvckBie3OjeR?= NGnxWIoyODBidV2= Mn;sTY5pcWKrdHnvckBw\iCZboSvZoV1[S2lYYPlbY4hcW5iaIXtZY4hVFNzN{TUJINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKHB{MWfBSlEwS0mSMTDwdo91\WmwIHzleoVtKGG2IEGwNEB2VSCkeTDX[ZN1\XKwIHLsc5R1cW6p NUXOWHJSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyPVEzOzVpPkKxNlkyOjN3PD;hQi=>
LS174T Mm\zSpVv[3Srb36gZZN{[Xl? NGnKNYYyODBidV2= NFz5TZRKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPhd4VqdiCrbjDoeY1idiCOU{G3OHQh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHP5Z4xqdiCEMTDwdo91\WmwIHzleoVtKGG2IEGwNEB2VSCkeTDX[ZN1\XKwIHLsc5R1cW6p MmraQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{OUGyN|UoRjJzMkmxNlM2RC:jPh?=
LS174T MorrSpVv[3Srb36gZZN{[Xl? Mn7yNVAxKHWP Mn24TY5pcWKrdHnvckBw\iCZboSvZoV1[S2lYYPlbY4hcW5iaIXtZY4hVFNzN{TUJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiClLV35Z{Bxem:2ZXnuJIxmfmWuIHH0JFExOCC3TTDifUBY\XO2ZYLuJIJtd3S2aX7n MkDIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{OUGyN|UoRjJzMkmxNlM2RC:jPh?=
LS174T MVnGeY5kfGmxbjDhd5NigQ>? NWjmUYIxOTByIIXN MV\Jcohq[mm2aX;uJI9nKFewdD;i[ZRiNWOjc3XpckBqdiCqdX3hckBNWzF5NGSgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKEO7Y3zpckBFOSCycn;0[YlvKGyndnXsJIF1KDFyMDD1UUBjgSCZZYP0[ZJvKGKub4T0bY5o NIG3WlI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK5NVI{PSd-MkGyPVEzOzV:L3G+
HEK293T MkXBSpVv[3Srb36gZZN{[Xl? MY[xNFAhfU1? NU\t[XF6OTJiaILz M{DOS2lvcGmkaYTpc44hd2ZiTHnDcE1qdmS3Y3XkJHdvfC:kZYThMYNie2WrbjDpckBpfW2jbjDISWszQTOWIHPlcIx{KHS{YX7z[oVkfGWmIIfpeIghXE:SIF\sZZNpKGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJIx2[2moZYLhd4Uh\XiycnXzd4lwdiCjdDCxNFAhfU1iYX\0[ZIhOTJiaILzJIJ6KGS3YXygcJVkcW[ncnHz[UBz\XCxcoTldkBie3OjeR?= NH3s[lY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK5NVI{PSd-MkGyPVEzOzV:L3G+
SW480 NXrXVlhYS3m2b4TvfIlkcXS7IHHzd4F6 MUixNEB2VQ>? NUO1bWpIPCCmYYnz MkP3R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV3c1QDBiY3XscJMh[XRiMUCgeW0h\m:{IESg[IF6eyCkeTD2bU1k\WyuIHPlcIwhfmmjYnnsbZR6KGGwYXz5d4l{ MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ7MUKzOUc,OjF{OUGyN|U9N2F-
LS 174T M3TydmZ2dmO2aX;uJIF{e2G7 M3KzdVExOCC3TR?= MWDJcohq[mm2aX;uJI9nKFewdD;i[ZRiNWOjc3XpckBqdiCqdX3hckBNWyBzN{TUJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCWQ1[0JJBzd3SnaX6gcIV3\WxiYYSgNVAxKHWPIHL5JHdme3Sncn6gZoxwfHSrbne= M{e5fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmxNlM2Lz5{MUK5NVI{PTxxYU6=
LS 174T MWrGeY5kfGmxbjDhd5NigQ>? M3nh[VExOCC3TR?= M2W2fmlvcGmkaYTpc44hd2ZiV370M4JmfGFvY3Hz[YlvKGmwIHj1cYFvKEyVIEG3OHQh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIID5[49xfXN{IIDyc5RmcW5ibHX2[Ywh[XRiMUCwJJVOKGK7IGfld5Rmem5iYnzveJRqdmd? M3i1d|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmxNlM2Lz5{MUK5NVI{PTxxYU6=
HEK293T M2m3fGZ2dmO2aX;uJIF{e2G7 M13YZ|ExOCC3TR?= MWOxNkBpenN? Mlf2TY5pcWKrdHnvckBw\iCZboSvZoV1[S2lYYPlbY4hcW5iaIXtZY4hUEWNMkmzWEBk\WyuczD0doFve2[nY4Tl[EB4cXSqIGTPVEBHdGG|aDDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiCudXPp[oVz[XOnIHX4dJJme3Orb36gZZQhOTByIIXNJIFnfGW{IEGyJIhzeyCkeTDkeYFtKGy3Y3nm[ZJie2VicnXwc5J1\XJiYYPzZZk> M4\ZUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmxNlM2Lz5{MUK5NVI{PTxxYU6=
32Dcl3 NFfySplHfW6ldHnvckBie3OjeR?= MWGxNEB2VQ>? NU\neJQ6OSCqch?= NWT2dYV4WmGmaX;wdo91\WO2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgNEB1dyB5IFf5JIdidW2jIILh[IlifGmxbj3pcoR2[2WmIHTlZZRpKGmwIH3veZNmKDN{RHPsN{Bk\WyuczDheEAyOCC3TTD0doVifGWmIEGgbJIheHKrb4KgeI8hcXK{YXTpZZRqd25iYX\0[ZIhPyCmYYnzJIJ6KG2rY4Lvd4NweHl? NUjBfm1kRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4NlYzPTNpPkKxPFI3OjV|PD;hQi=>
HeLa NV[5fopiSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MV[0PEBpenN? NWTPOFBJSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;MD6wNFAxOjJ3zszNMi=> NEnjOZQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi1NVA5Oyd-MkG4OVExQDN:L3G+
SK-N-SH MUfBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? Mm\HOFghcHK| NIPEcolCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPLMW4uW0hiY3XscJMh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME2wMlAxODB2MEROwG0v NV3rW5h5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4OVExQDNpPkKxPFUyODh|PD;hQi=>
A549 MmG1RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M1PiVVQ5KGi{cx?= NGO0Wm1CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZZN{\XO|ZXSgZZMh[2WubDD2bYFjcWyrdImgZYZ1\XJiNEigbJJ{KGK7IGPSRkBie3OjeTygTWM2OD1yLkCwNFA1PDgQvF2u NYmwWIRbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4OVExQDNpPkKxPFUyODh|PD;hQi=>
MCF7 MkX4RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NXLTVGhkPDhiaILz MUHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgZ4VtdCC4aXHibYxqfHliYX\0[ZIhPDhiaILzJIJ6KFOUQjDhd5NigSxiSVO1NF0xNjByMEC3PVHPxE1w MkC4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6NUGwPFMoRjJzOEWxNFg{RC:jPh?=
RAW264.7 MljyRY51cWmwZnzhcY1ifG:{eTDhd5NigQ>? NEHXOWQ2OCC3TR?= NH;2OGoyNjViaILz NHTQNXZCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKG2xdYPlJHJCXzJ4ND63JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgWG5HNWGucHjhJJBzd2S3Y4Tpc44h[XRiNUCgeW0h[W[2ZYKgNU42KGi{czDifUBGVEmVQR?= NYTRdlJ7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwPFg{ODlpPkKyNFg5OzB7PD;hQi=>
HEK293 MnzDSpVv[3Srb36gZZN{[Xl? MWLJcohq[mm2aX;uJI9nKFSQRnHsdIhiNWGldHn2ZZRm\CCQRj3rZZBx[UJiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEmFNUC9NE46QM7:TT6= NHLQdVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkGxOVg{QSd-MkKxNVU5Ozl:L3G+
Hepa-1c1c7 MmPkSpVv[3Srb36gZZN{[Xl? M2TsRmlv\HWldHnvckBw\iCTUkGgZYN1cX[rdImgbY4hdW:3c3WgTIVx[S1zY{HjO{Bk\WyuczD1d4lv\yCPVGSgZZMhe3Wkc4TyZZRmKGK7IIPw[YN1em:yaH;0c41mfHK7LDDDSF0zOc7:TT6= MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjFzNUizPUc,OjJzMUW4N|k9N2F-
RAW264.7 MUTBcpRqcW6obHHtcYF1d3K7IHHzd4F6 MUizNEBucW6| MUjBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhVFCVLXnu[JVk\WRiTl:gdJJw\HWldHnvckBxemWrbnP1ZoF1\WRiZn;yJFMxKG2rboOgZoVnd3KnIFzQV{BkcGGubHXu[4UhdWWjc4Xy[YQh[W[2ZYKgNlQhcHK|IHL5JGdzcWW|czDt[ZRpd2RuIFnDOVA:OzBwN{JOwG0v MnPzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzMUW4N|koRjJ{MUG1PFM6RC:jPh?=
B16F10 NF\NeIlCdnSrbXXsZY5w\2WwaXOgZZN{[Xl? NGPxcGIzODBidV2= M{niOVczKGi{cx?= NFzCdJdCdnSrbXXsZY5w\2WwaXOgZYN1cX[rdImgbY4hdW:3c3WgZZN{\XO|ZXSgZZMh[2WubIXsZZIhdWWuYX7pckBkd262ZX70JGIyPkZzMDDj[YxteyCjdDCyNFAhfU1iYX\0[ZIhPzJiaILzJJNx\WO2cn;wbI91d22ndILpZ{BidmGueYPpdy=> MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF6OUK3Nkc,OjJzOEmyO|I9N2F-
HEK293 NG\GSnFHfW6ldHnvckBie3OjeR?= NE\TVWZKdmirYnn0bY9vKG:oIGTOSk1idHCqYTDhZ5RqfmG2ZXSgUmYuc2GycHHCJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR|UxRTBwOUlOwG0v Mn7PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|OE[1OlQoRjJ{M{i2OVY1RC:jPh?=
Hepa-1c1c7 NUXxO2g6TnWwY4Tpc44h[XO|YYm= NIriUoxKdmS3Y4Tpc44hd2ZiUWKxJIlvKG2xdYPlJGhmeGFvMXOxZ|ch[2WubIOgZZN{\XO|ZXSgZZMh[2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{Bld3WkbHWgVXIyKGmwZIXjeIlwdiCkeTDNWHQh[XO|YYmsJGNFRTJzzszNMi=> MmXUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|OE[1OlQoRjJ{M{i2OVY1RC:jPh?=
RAW264.7 NUXFVXhETnWwY4Tpc44h[XO|YYm= MWSxPEBpenN? M4r1N2lvcGmkaYTpc44hd2ZiaV7PV{BqdiCOUGOtd5RqdXWuYYTl[EBud3W|ZTDSRXczPjRwNzDj[YxteyCjZoTldkAyQCCqcoOsJGlEPTB;MkOuNu69VS5? MmWxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|OE[1OlQoRjJ{M{i2OVY1RC:jPh?=
RAW264.7 M1HlWGFvfGmrbn\sZY1u[XSxcomgZZN{[Xl? MXizNEBucW6| Mn:5RY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEySUz3pcoR2[2WmIF7PJJBzd2S3Y4Tpc44heHKnaX7jeYJifGWmIH\vdkA{OCCvaX7zJI1m[XO3cnXkJFI1KGi{czDwc5N1KEySUzDjbIFtdGWwZ3WgZpkhT3KrZYPzJI1mfGixZDygTWM2OD1|MD63Ne69VS5? NHXLPYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkO4OlU3PCd-MkKzPFY2PjR:L3G+
NIH/3T3 NVTCbZRpS3m2b4TvfIlkcXS7IHHzd4F6 M2\MbFk3KGi{cx?= Ml7CR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgUmlJNzOWMzDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVI1NjJ{zszNMi=> NGDCfZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0PVM6Oid-MkK3OFk{QTJ:L3G+
HEK293 MoPYSpVv[3Srb36gZZN{[Xl? M1jFTWlvcGmkaYTpc44hd2ZiVF7GZYxxcGFvaX7keYNm\CCQRj3rZZBx[UJiYXP0bZZqfHliZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEmFNUC9NE46QM7:TT6= M4W0WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MUSyN|IxLz5{M{G0NlMzODxxYU6=
Hepa-1c1c7 MXvGeY5kfGmxbjDhd5NigQ>? Mny0OFghcHK| NEjVfnJKdmS3Y4Tpc44hd2ZicYXpco9v\SC{ZXT1Z5Rie2VvMTDhZ5Rqfmm2eTDpckBud3W|ZTDI[ZBiNTGlMXO3JINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFPEQVIy|ryPLh?= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF2MkOyNEc,OjNzNEKzNlA9N2F-
RAW 264.7 M{Tye2Z2dmO2aX;uJIF{e2G7 NUjyRYdXOzBibXnudy=> MoPBTY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDpUm9UKGGldHn2bZR6KGmwIH3veZNmKFKDVzCyOlQvPyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJG5QKHC{b3T1Z5Rqd25icILleJJm[XSnZDD3bZRpKGOxbYDveY5lKG[xcjCzNEBucW6|IHLl[o9z\SCOUGOgZ4hidGynbnflJIFnfGW{IEK0JIhzeyCkeTDHdolme3NicnXh[4VvfCCvZYToc4QtKEmFNUC9NlMvOs7:TT6= NV;CWG9PRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOFI{OjBpPkKzNVQzOzJyPD;hQi=>
MCF7 NH72VJJHfW6ldHnvckBie3OjeR?= M1\pPV4yODBidV2= M{jLOWFkfGm4YYTpc44hd2ZiU1nSWFEhcW5iaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h\G:6b4L1ZolkcW5vaX7keYNm\CCjY3X0fYwueDV|IHzleoVtKGG2IE6xNFAhfU1iYX\0[ZIhPiCqcoOgZpkhX2W|dHXyckBjdG:2dHnu[{BidmGueYPpdy=> NVTEbGZHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzNVY5ODNpPkKzN|E3QDB|PD;hQi=>
HL60 NYexPYJKS3m2b4TvfIlkcXS7IHHzd4F6 NYLJZ2s1PDhiaILz MoiyR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCvZXHzeZJm\CCjZoTldkA1QCCqcoOgZpkhYFSWIHHzd4F6NCCLQ{WwQVMyNjkQvF2u M3zpWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUS3PFQ{Lz5{M{W0O|g1OzxxYU6=
HeLa NUX2PZZUTnWwY4Tpc44h[XO|YYm= NUHwPYgyUW6qaXLpeIlwdiCxZjDzcYFlOy92IIPp[45idGmwZzDwZZRpf2G7IHnuJIh2dWGwIFjlUIEh[2WubIOgZZN{\XO|ZXSgZZMhdWWjc4Xy[YQh[XNibH;3[ZN1KGOxbnPlcpRz[XSrb36gdoVyfWm{ZXSgOVAhfG9iNkCgKUBqdmirYnn0bY9vKG:oIGTHSk1j\XSjIHnu[JVk\WRibIXjbYZmemG|ZTD0doVifGWmIEOwJI1qdnNiYnXmc5JmKGmwZIXjeIlwdixiTVXEQVM{|ryPLh?= NHjFZYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W0O|g1Oyd-MkO1OFc5PDN:L3G+
HeLa M4XydGZ2dmO2aX;uJIF{e2G7 NELMOmxKdmirYnn0bY9vKG:oIFjl[IdmcG:pIIPp[45idGmwZzDwZZRpf2G7IHnuJIh2dWGwIFjlUIEh[2WubIOgZZN{\XO|ZXSgZZMhdWWjc4Xy[YQh[XNibH;3[ZN1KGOxbnPlcpRz[XSrb36gdoVyfWm{ZXSgOVAhfG9iNkCgKUBqdmirYnn0bY9vKG:oIGDNRUBqdmS3Y3XkJIx2[2moZYLhd4UhfHKnYYTl[EA{OCCvaX7zJIJm\m:{ZTDpcoR2[3Srb36sJG1GTD12MN88UU4> M1HuV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUS3PFQ{Lz5{M{W0O|g1OzxxYU6=
HeLa NUWzTFJxTnWwY4Tpc44h[XO|YYm= MUTJcohq[mm2aX;uJI9nKEinZHflbI9oKHOrZ37hcIlv\yCyYYToe4F6KGmwIHj1cYFvKEinTHGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCSTVGgbY5lfWOnZDDseYNq\mW{YYPlJJRz\WG2ZXSgN|AhdWmwczDi[YZwemViaX7keYN1cW:wLDDJR|UxRTRyzszNMi=> NVLoUZU4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1OFc5PDNpPkKzOVQ4QDR|PD;hQi=>
HeLa M1fhZ2Z2dmO2aX;uJIF{e2G7 MYrJcohq[mm2aX;uJI9nKE6xdHPoJJNq\26jbHnu[{Bx[XSqd3H5JIlvKGi3bXHuJGhmVGFiY3XscJMh[XO|ZYPz[YQh[XNibXXhd5Vz\WRiYYOgcI94\XO2IHPvcoNmdnS{YYTpc44hemWzdXny[YQhPTBidH:gOlAhLSCrbnjpZol1cW:wIH;mJHBOSSCrbnT1Z4VlKGy3Y3nm[ZJie2VidILlZZRm\CB|MDDtbY5{KGKnZn;y[UBqdmS3Y4Tpc44tKE2HRE20NO69VS5? NX7Pdo9qRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1OFc5PDNpPkKzOVQ4QDR|PD;hQi=>
HeLa NGXOXFlHfW6ldHnvckBie3OjeR?= M1vDXGlvcGmkaYTpc44hd2ZiTn;0Z4ghe2mpbnHsbY5oKHCjdHj3ZZkhcW5iaIXtZY4hUGWOYTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKFCPQTDpcoR2[2WmIHz1Z4ln\XKjc3WgeJJm[XSnZDCzNEBucW6|IHLl[o9z\SCrbnT1Z5Rqd25uIFnDOVA:PDEQvF2u M{nMelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUS3PFQ{Lz5{M{W0O|g1OzxxYU6=
SK-MEL NU\OXoxTS3m2b4TvfIlkcXS7IHHzd4F6 MnTJOFghcHK| MmK3R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2suVUWOIHPlcIx{KGG|c3Xzd4VlKGG|IHfyc5d1cCCrbnjpZol1cW:wIH3lZZN2emWmIHHmeIVzKDR6IHjyd{BjgSC[VGSgZZN{[XluIFnDOVA:PDdwM988UU4> NGH6OoY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W0O|g1Oyd-MkO1OFc5PDN:L3G+
HL60 NFLtUpJEgXSxdH;4bYNqfHliYYPzZZk> MVfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzMEBGTDVyPUKy{txONg>? NH;aWYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W4Nlc4QCd-MkO1PFI4Pzh:L3G+
Huh7.5.1 NYDDfWZkS3m2b4TvfIlkcXS7IHHzd4F6 NXSy[mJuPzJiaILz MoKwR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJVpPy53LkGgZ4VtdHNiYX\0[ZIhPzJiaILzMEBKSzVyPUGwMlbPxE1w M4nhd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkezNlI2Lz5{M{[3N|IzPTxxYU6=
MCF7 M37uZ2N6fG:2b4jpZ4l1gSCjc4PhfS=> NHr4Z5E4OiCqcoO= NHPWR2VEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBme3S{b3flckBz\WOncITvdkBxd3OrdHn2[UBOS0Z5IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NlgvODgQvF2u MlS3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NkC1PVAoRjJ|OE[wOVkxRC:jPh?=
SKBR3 MmLyR5l1d3SxeHnjbZR6KGG|c3H5 NGHOV244OiCqcoO= M3[2bGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJIV{fHKxZ3XuJJJm[2WydH;yJI5m\2G2aY\lJHNMSlJ|IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9OFEvPDMQvF2u M4O4[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OE[wOVkxLz5{M{i2NFU6ODxxYU6=
ScN2a-cl3 NFvESlJHfW6ldHnvckBie3OjeR?= M4TuUFUh\GG7cx?= M{nqRWlvcGmkaYTpc44hd2ZiUl3MJJBzcW:wIIDyc5RmcW5iaX7m[YN1\WRiaX6gcY92e2ViZHn2bYRqdmdiU3PONoEu[2x|IHPlcIx{KGW6cILld5NqdmdiZoXscEBt\W6pdHigcY92e2ViUILQJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCxZjDQdnB{[yCuZY\lcEBi\nSncjC1JIRigXNiYomgW4V{fGW{bjDicI91fGmwZzDhcoFtgXOrczygSWM2OD1yLkW4{txONg>? MnfmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzOEO1PFkoRjJ2MUizOVg6RC:jPh?=
ScN2a-cl3 NWDhUo9ITnWwY4Tpc44h[XO|YYm= MnzyOUBl[Xm| MlzOTY5pcWKrdHnvckBw\iCUTVygdJJqd25icILveIVqdiCrbn\lZ5Rm\CCrbjDtc5V{\SCmaY\p[Ilv\yCVY16yZU1kdDNiY3XscJMh\XiycnXzd4lv\yCodXzsJIxmdme2aDDtc5V{\SCScmCgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKG:oIGDyVJNkKGyndnXsJIFnfGW{IEWg[IF6eyCkeTDFUGlUSSxiRVO1NF0{NjB4zszNMi=> M{LaVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUizOVg6Lz5{NEG4N|U5QTxxYU6=
SH-SY5Y NYTrNIl5VmW3cn;wdo91\WO2aY\lJIF{e2G7 NULISYl4OTBidV2= MoHPNlQhcHK| MkfhUoV2em:ycn;0[YN1cX[nIHHjeIl3cXS7IHnuJIh2dWGwIGPIMXN[PVliY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDINm8zNWmwZIXj[YQhemWmdXP0bY9vKG:oIHPlcIwhfmmjYnnsbZR6KGG2IEGwJJVOKGGodHXyJFI1KGi{czDifUBxcGG|ZT3jc451emG|dDDtbYNzd2e{YYDoJIFv[Wy7c3nz NXPEeWxPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOlk2OTVpPkK0NlY6PTF3PD;hQi=>
HEK293 NEHiclVHfW6ldHnvckBie3OjeR?= Mn\TOkBpenN? NUe2dnNIUW6qaXLpeIlwdiCxZjDUUmYu[WyyaHGtbY5lfWOnZDDOSk1s[XCyYVKgZYN1cX[rdImgbY4hUEWNMkmzJINmdGy|IHHmeIVzKDZiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OD1{LkZOwG0v MlnVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7OUK3NFIoRjJ2OUmyO|AzRC:jPh?=
Hepa-1c1c7 M4rpR2Z2dmO2aX;uJIF{e2G7 NXn1UGF3PDhiaILz MYLJcoR2[3Srb36gc4YheXWrbn;u[UBz\WS3Y4Thd4UhOSCrbjDtc5V{\SCKZYDhMVFkOWN5IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEOGPUKx{txONg>? MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl7MkewNkc,OjR7OUK3NFI9N2F-
HeLa NIrlbZZHfW6ldHnvckBie3OjeR?= M4HEZ|ExKHSxIEGwNEB2VQ>? M3LSblI1KGi{cx?= NEPUXYJKdmS3Y4Tpc44hd2ZiU2[0NEBxem:vb4Tldk1u\WSrYYTl[EBtfWOrZnXyZZNmKGenbnWg[ZhxemW|c3nvckBqdiCqdX3hckBJ\UyjIHPlcIx{KGG2IEGwJJRwKDFyMDD1UUBi\nSncjCyOEBpenNiYomgVnQuWEOUIHHuZYx6e2m| M2rtNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUS3OlA1Lz5{NUG0O|YxPDxxYU6=
COLO205 NEHBV3JCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NIfLNmNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFPPUG8zODViY3XscJMh[nliTWTUJIF{e2G7LDDJR|UxRTJ|LkZOwG0v M3vkOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEW1OFg3Lz5{NUS1OVQ5PjxxYU6=
MGC803 NFztfnJCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M4nsN2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVfDPFA{KGOnbHzzJIJ6KE2WVDDhd5NigSxiSVO1NF01Os7:TT6= Mn\JQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2NUW0PFYoRjJ3NEW1OFg3RC:jPh?=
MDA468 MnXWRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M4\pT2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBOFY5KGOnbHzzJIJ6KE2WVDDhd5NigSxiSVO1NF01PS5{zszNMi=> M2TBV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEW1OFg3Lz5{NUS1OVQ5PjxxYU6=
HeLa MYHBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NYDLeWpJPDhiaILz M4[5dGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSHXMZUBk\WyuczDhd5Nme3OnZDDhd{Bk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTR5LkpOwG0v M2DNTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEG3O|czLz5{NUixO|c4OjxxYU6=
RAW264.7 NE\ZOpJCdnSrLXnu[oxidW2jdH;yfUBie3OjeR?= NYDtb4lrOTBibV2= M3TvOVExKG2rboO= NWnDcZV7SW62aT3pcoZt[W2vYYTvdpkh[WO2aY\peJkhcW5ibX;1d4UhWkGZMk[0Mlch[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDMVHMucW6mdXPl[EBKVDZicILv[JVkfGmxbjDheEAyOCCvTTDwdoUucW6ldXLheIVlKG[xcjCxNEBucW6|IHLl[o9z\SCOUGOgd5RqdXWuYYTpc44h\m:{IEK0JIhzeyCkeTDFUGlUSSCvZYToc4Q> M4HkNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MES4O|g5Lz5{NkC0PFc5QDxxYU6=
RAW264.7 MmjwRY51cS2rbn\sZY1u[XSxcomgZZN{[Xl? NV;lUYl4OTBibV2= NVHHPWl6OTBibXnudy=> M2niR2FvfGlvaX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKG2xdYPlJHJCXzJ4ND63JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgWG5H[WyyaHGgdJJw\HWldHnvckBifCBzMDDtUUBxemVvaX7jeYJifGWmIH\vdkAyOCCvaX7zJIJm\m:{ZTDMVHMhe3SrbYXsZZRqd25iZn;yJFI1KGi{czDifUBGVEmVQTDt[ZRpd2R? M1HjfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MES4O|g5Lz5{NkC0PFc5QDxxYU6=
HaCaT MlS2SpVv[3Srb36gZZN{[Xl? MXSxJJVO NWftRod7OSCqch?= NH7hSJlC[3SrdnH0bY9vKG:oIGPJVnQyKGmwIHj1cYFvKEijQ3HUJINmdGy|IHHzd4V{e2WmIHHzJGg1UzF4IHTlZYNmfHmuYYTpc44h[XRiMTD1UUBi\nSncjCxJIhzKGK7IGfld5Rmem5iYnzveJRqdmdibXX0bI9l MmTkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ4OEmzOVIoRjJ4Nki5N|UzRC:jPh?=
HEK293 NHvnTXBHfW6ldHnvckBie3OjeR?= NV\5R2IzSW62YXfvcol{fCCjY4Tpeol1gSCjdDDyZZQhXFKSQUGg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFHJWGMucW6mdXPl[EBqdmO{ZXHz[UBw\iCrboTyZYNmdGy3bHHyJINidGOrdX2gcIV3\WxuIFnDOVA:OC55Nd88UU4> M4KwPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{WwNlU5Lz5{Nke1NFI2QDxxYU6=
HEK293 NHzNd5RHfW6ldHnvckBie3OjeR?= M1n1XGFod26rc4SgZYN1cX[rdImgZZQhemG2IGTSVGEyKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJIlvfHKjY3XscJVt[XJiY3HsZ4l2dSCuZY\lcEBqdiCjYoPlcoNmKG:oIFHJWGMtKEmFNUC9NU41|ryPLh?= MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjd3MEK1PEc,OjZ5NUCyOVg9N2F-
HEK293 MX3GeY5kfGmxbjDhd5NigQ>? NIfGWFBC\2:waYP0JIFkfGm4aYT5JIF1KHKjdDDUVnBCOSCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBqdnS{YXPlcIx2dGG{IHPhcINqfW1ibHX2[YwhcW5icILld4Vv[2Vib3[gRWlVSyxiSVO1NF0zNjd2zszNMi=> NVnrN2lxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3OVAzPThpPkK2O|UxOjV6PD;hQi=>
HEK293 MonjSpVv[3Srb36gZZN{[Xl? MXLBcpRi\2:waYP0JIFkfGm4aYT5JIF1KHKjdDDUVnBCOSCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yh[WyueXygbZNwfGirb3P5ZY5ifGVvaX7keYNm\CCrbnPy[YF{\SCxZjDpcpRz[WOnbHz1cIFzKGOjbHPpeY0hdGW4ZXysJGlEPTB;MUmuPe69VS5? MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjd3MEK1PEc,OjZ5NUCyOVg9N2F-
U373-MAGI NFTjbZhCdnSrdnnyZYwh[XO|YYm= NGXjNoQ2OCC3TR?= MkHDNkBpenN? M4TLZ3BwfGWwdHnheIlwdiCxZjC1MWF7[S2FLXnu[JVk\WRiYX70bZZqemGuIHHjeIl3cXS7IHHnZYlve3RiVmPWMWcheHOndXTveJlx\WRiSFnWMVEhVkx2LUOgbY5n\WO2ZXSgbY4hcHWvYX6gWVM4Oy2PQVfJJINmdGy|IHHzd4V{e2WmIHHzJFUuSXqjLVOgSWM2OCCjdDC1NEB2VSCycnXpcoN2[mG2ZXSg[o9zKDJiaILzJIZwdGyxd3XkJIJ6KDVvQYrhMWMh[WSmaYTpc44h\m:{IEKgbJJ{KGGwZDDzeYJ{\XG3ZX70JJZqemGuIHnu[oVkfGmxbjDt[YF{fSxiRVO1NF0yOM7:TT6= NXfMRYk{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexNVczPjBpPkK3NVE4OjZyPD;hQi=>
U373-MAGI MmLrRY51cX[rcnHsJIF{e2G7 NE\afpQ2OCC3TR?= NUHTUIY6PCCqcoO= NXTne4F2SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhXlOYLVegdJNmfWSxdInw[YQhUEmYLUGgUmw1NTNiaX7m[YN1\WRiaX6gbJVu[W5iVUO3N{1OSUeLIHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckB3cXKjbDDpcoZm[3Srdnn0fUBifCB3MDD1UUBqdmO3YnH0[YQh\m:{IESgbJJ{KHC{aX;yJJRwKH[rcnHsJIlv\mWldHnvckBu\WG|dYLl[EBifCB5MjDodpMheG:|dDDpcoZm[3Srb36gZpkh\myxdzDjfZRwdWW2cnnjJIFv[Wy7c3nz NG\Se3c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{GxO|I3OCd-MkexNVczPjB:L3G+
U373-MAGI MnTqSpVv[3Srb36gZZN{[Xl? M2HtcVIxOCC3TR?= MnrLOkBpenN? M{fQ[XJm\HWldHnvckBqdiCmR2TQJIxmfmWuIHnuJIh2dWGwIGWzO|MuVUGJSTDj[YxteyCjdDCyNFAhfU1iYX\0[ZIhPiCqcoOgZpkhVENvTWOvUXMh[W6jbInzbZM> MljLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzMUeyOlAoRjJ5MUG3NlYxRC:jPh?=
U373-MAGI MXvGeY5kfGmxbjDhd5NigQ>? MlnUNlAxKHWP MXq2JIhzew>? NVHEUmk5WmWmdXP0bY9vKGmwIHTUWHAhdGW4ZXygbY4hcHWvYX6gWVM4Oy2PQVfJJINmdGy|IHH0JFIxOCC3TTDh[pRmeiB4IHjyd{BjgSCOQz3NV{9OWyCjbnHsfZNqew>? NXvNOox5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexNVczPjBpPkK3NVE4OjZyPD;hQi=>
U373-MAGI M1HwZWFvfGm4aYLhcEBie3OjeR?= MYSyOUB1dyBzMECgeW0> MVqyJIhzew>? NUfpV|k4WG:2ZX70bYF1cW:wIH;mJFUuSXqjLVOtbY5lfWOnZDDhcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDWV3YuTyCyc3X1[I91gXCnZDDITXYuOSCQTEStN{Bqdm[nY4Tl[EBqdiCqdX3hckBWOzd|LV3BS2kh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwII\pdoFtKGmwZnXjeIl3cXS7IHH0JFI2KHSxIEGwNEB2VSCycnXpcoN2[mG2ZXSg[o9zKDJiaILzJIZwdGyxd3XkJIJ6KDVvQYrhMWMh[WSmaYTpc44h\m:{IEKgbJJ{KGGwZDDzeYJ{\XG3 MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzFzN{K2NEc,OjdzMUeyOlA9N2F-
U373-MAGI NIPYcGJCdnSrdnnyZYwh[XO|YYm= M2DxU|IhfG9iODDodpM> MY\BcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDWV3YuTyCyc3X1[I91gXCnZDDITXYuOSCQTEStN{Bqdm[nY4Tl[EBqdiCqdX3hckBWOzd|LV3BS2kh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwII\pdoFtKGmwZnXjeIl3cXS7IIDy[Ylv[3WkYYTl[EBnd3JiMjD0c{A5KGi{czDmc4xtd3enZDDifUB3cXKjbDDpcoZm[3Srb36gcYVie3W{ZXSgZZQhPzJiaILzJJBwe3RiaX7m[YN1cW:wIHL5JIZtd3diY4n0c41mfHKrYzDhcoFtgXOrcx?= MkP5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzMUeyOlAoRjJ5MUG3NlYxRC:jPh?=
U373-MAGI NF[wc3NHfW6ldHnvckBie3OjeR?= MUGyNFAhfU1? MVe2JIhzew>? MX7S[YR2[3Srb36gbY4h\EOWUDDs[ZZmdCCrbjDoeY1idiCXM{ezMW1CT0liY3XscJMh[XRiMkCwJJVOKGGodHXyJFYhcHK|IHL5JGxENU2VL13TJIFv[Wy7c3nz Mo\2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzMUeyOlAoRjJ5MUG3NlYxRC:jPh?=
U373-MAGI M4j5WGZ2dmO2aX;uJIF{e2G7 NVWzVGZmPTBidV2= NVfwTHpqOiCqcoO= M4ftSXJm\HWldHnvckBqdiCmQ2TQJIxmfmWuIHnuJIh2dWGwIGWzO|MuVUGJSTDj[YxteyCjdDC1NEB2VSCycnXpcoN2[mG2ZXSg[o9zKDJiaILzJIZwdGyxd3XkJIJ6KDVvYYrhMWMh[WSmaYTpc44hdWWjc4Xy[YQh[W[2ZYKgOEBpenNiYomgUGMuVVNxTWOgZY5idHm|aYO= NWH1R3I5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexNVczPjBpPkK3NVE4OjZyPD;hQi=>
U373-MAGI MXXGeY5kfGmxbjDhd5NigQ>? NWHLPIhoOjByIIXN MmP6OkBpenN? MWfS[YR2[3Srb36gbY4h\EGWUDDs[ZZmdCCrbjDoeY1idiCXM{ezMW1CT0liY3XscJMh[XRiMkCwJJVOKGGodHXyJFYhcHK|IHL5JGxENU2VL13TJIFv[Wy7c3nz MlzNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzMUeyOlAoRjJ5MUG3NlYxRC:jPh?=
U373-MAGI NFTWbIhHfW6ldHnvckBie3OjeR?= MVWyNFAhfU1? M4\hPFIhcHK| NWPqR5dmWmWmdXP0bY9vKGmwIHTSS3UuXFBibHX2[YwhcW5iaIXtZY4hXTN5Mz3NRWdKKGOnbHzzJIF1KDJyMDD1UUBxemWrbnP1ZoF1\WRiZn;yJFIhcHK|IH\vcIxwf2WmIHL5JFUu[XqjLVOgZYRlcXSrb36gcYVie3W{ZXSgZYZ1\XJiNDDodpMh[nliTFOtUXMwVVNiYX7hcJl{cXN? NXWwVIRNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexNVczPjBpPkK3NVE4OjZyPD;hQi=>
U373-MAGI MYLGeY5kfGmxbjDhd5NigQ>? NFrkWJk2OCC3TR?= NXrvdYxuOiCqcoO= NHfzfG5KdmO{ZXHz[UBqdiB3LXH6ZU1lS1SSL3TDWHAhemG2aX:gbY4hcHWvYX6gWVM4Oy2PQVfJJINmdGy|IHH0JFUxKHWPIIDy[Ylv[3WkYYTl[EBnd3JiMjDodpMh\m:ubH;3[YQh[nliNT3hfoEu\ENiYXTkbZRqd25ibXXhd5Vz\WRiYX\0[ZIhPCCqcoOgZpkhVENvTWOvUXMh[W6jbInzbZMhemWuYYTpeoUhfG9iNT3hfoEu\EN? NIHBXlQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{GxO|I3OCd-MkexNVczPjB:L3G+
U373-MAGI MUfGeY5kfGmxbjDhd5NigQ>? MlTjNlAxKHWP NIDjcZIzKGi{cx?= M2jWcnJm\HWldHnvckBqdiCmQ2TQJIxmfmWuIHnuJIh2dWGwIGWzO|MuVUGJSTDj[YxteyCjdDCyNFAhfU1icILlbY5kfWKjdHXkJIZweiB{IHjyd{Bnd2yub4fl[EBjgSB3LXH6ZU1EKGGmZHn0bY9vKG2nYYP1doVlKGGodHXyJFQhcHK|IHL5JGxENU2VL13TJIFv[Wy7c3nzJJJmdGG2aY\lJJRwKDVvYYrhMWM> MlS0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzMUeyOlAoRjJ5MUG3NlYxRC:jPh?=
U373-MAGI NFfQb2tCdnSrdnnyZYwh[XO|YYm= MnG3OVAhfU1? NFf6d2MzKHSxIEeyJIhzew>? MVjQc5RmdnSrYYTpc44hd2ZiNT3BfoEuSy2rbnT1Z4VlKGGwdHn2bZJidCCjY4Tpeol1gSCjZ3HpcpN1KF[VVj3HJJB{\XWmb4T5dIVlKEiLVj2xJG5NPC1|IHnu[oVkfGWmIHnuJIh2dWGwIGWzO|MuVUGJSTDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hXTVvZ3HnJIxmfmWuIHH0JFUxKHWPIHHmeIVzKDJidH:gO|IhcHK|IHL5JJFRS1JibXX0bI9l MkjmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzMUeyOlAoRjJ5MUG3NlYxRC:jPh?=
U373-MAGI NVLNVIR2SW62aY\pdoFtKGG|c3H5 NX\xNGVoPTBidV2= Mn\JOkBpenN? M{[wbGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JHZUXi2JIIDz[ZVld3S7cHXkJGhKXi1zIF7MOE0{KGmwZnXjeIVlKGmwIHj1cYFvKFV|N{OtUWFIUSClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6g[4FoKGyndnXsJIF1KDVyIIXNJI1m[XO3cnXkJIF1KDZiaILzJJBwe3RiaX7m[YN1cW:wIHL5JJFRS1JibXX0bI9l MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzFzN{K2NEc,OjdzMUeyOlA9N2F-
U373-MAGI NWXYTmtvTnWwY4Tpc44h[XO|YYm= M2fVXVIxOCC3TR?= NUDldlR[OiCqcoO= MmL1TY5kemWjc3WgbY4hPS2jenGt[GNVWC:mQ2TQJJJifGmxIHnuJIh2dWGwIGWzO|MuVUGJSTDj[YxteyCjdDCyNFAhfU1icILlbY5kfWKjdHXkJIZweiB{IHjyd{Bnd2yub4fl[EBjgSB3LXH6ZU1lSyCjZHTpeIlwdiCvZXHzeZJm\CCjZoTldkA1KGi{czDifUBNSy2PUz;NV{BidmGueYPpd{Bz\WyjdHn2[UB1dyB3LXH6ZU1lSw>? MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzFzN{K2NEc,OjdzMUeyOlA9N2F-
U373-MAGI NYPscYgyTnWwY4Tpc44h[XO|YYm= MWiyNFAhfU1? NYr6bYZCOiCqcoO= M3TBO3Jm\HWldHnvckBqdiB3LXH6ZU1lS1SSIHzleoVtKGmwIHj1cYFvKFV|N{OtUWFIUSClZXzsd{BifCB{MECgeW0heHKnaX7jeYJifGWmIH\vdkAzKGi{czDmc4xtd3enZDDifUA2NWG8YT3DJIFl\Gm2aX;uJI1m[XO3cnXkJIFnfGW{IESgbJJ{KGK7IFzDMW1UN02VIHHuZYx6e2m| M{XXUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUG3NlYxLz5{N{GxO|I3ODxxYU6=
U373-MAGI Mm\yRY51cX[rcnHsJIF{e2G7 Mof4OVAhfU1? NHf6XFczKHSxIEigbJJ{ NHTsbIZRd3SnboTpZZRqd25ib3[gOU1CgmFvQz3pcoR2[2WmIHHueIl3cXKjbDDhZ5Rqfmm2eTDh[4FqdnO2IG\TWk1IKHC|ZYXkc5R6eGWmIFjJWk0yKE6OND2zJIlv\mWldHXkJIlvKGi3bXHuJHU{PzNvTVHHTUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5idnnyZYwhcW6oZXP0bZZqfHliYYSgOVAhfU1icILlbY5kfWKjdHXkJIZweiB{IITvJFghcHK|IH\vcIxwf2WmIHL5JFUuSXqjLVOgZYRlcXSrb36g[o9zKDJiaILzJIFv\CC|dXLz[ZF2\W5? NITaZZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{GxO|I3OCd-MkexNVczPjB:L3G+
U373-MAGI M{\jUWFvfGm4aYLhcEBie3OjeR?= M4ntSVI2KHWP NWL2bFBsOiCqcoO= NYTwXHFFWG:2ZX70bYF1cW:wIH;mJFUuSXqjLXTDMYlv\HWlZXSgZY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgWnNXNUdicIPleYRwfHmyZXSgTGlXNTFiTly0MVMhcW6oZXP0[YQhcW5iaIXtZY4hXTN5Mz3NRWdKKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjD2bZJidCCrbn\lZ5Rqfmm2eTDheEAzPSC3TTDwdoVqdmO3YnH0[YQh\m:{IEKgbJJ{KG[xbHzve4VlKGK7IEWtRZpiNWSFIHHk[Il1cW:wIH\vdkAzKGi{czDhcoQhe3Wkc3XxeYVvfCC4 M3XpVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUG3NlYxLz5{N{GxO|I3ODxxYU6=
U373-MAGI MXHGeY5kfGmxbjDhd5NigQ>? MUC1NEB2VQ>? MofRNkBpenN? M3mxO2lv[3KnYYPlJIlvKDVvYYrhMYREXFBxZFPUVEBz[XSrbzDpckBpfW2jbjDVN|c{NU2DR1mgZ4VtdHNiYYSgOVAhfU1icILlbY5kfWKjdHXkJIZweiB{IHjyd{Bnd2yub4fl[EBjgSB3LXH6ZU1EKGGmZHn0bY9vKG2nYYP1doVlKGGodHXyJFQhcHK|IHL5JGxENU2VL13TJIFv[Wy7c3nzJJJmdGG2aY\lJJRwKDVvYYrhMWM> MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzFzN{K2NEc,OjdzMUeyOlA9N2F-
U373-MAGI NI\2N4tHfW6ldHnvckBie3OjeR?= M2jpeVUxKHWP MU[yJIhzew>? MkLIVoVlfWO2aX;uJIlvKGSFVGCgcIV3\WxiaX6gbJVu[W5iVUO3N{1OSUeLIHPlcIx{KGG2IEWwJJVOKHC{ZXnuZ5Vj[XSnZDDmc5IhOiCqcoOg[o9tdG:5ZXSgZpkhPS2jenGt[GMh[WSmaYTpc44hdWWjc4Xy[YQh[W[2ZYKgOEBpenNiYomgUGMuVVNxTWOgZY5idHm|aYOgdoVt[XSrdnWgeI8hPS2jenGt[GM> NWf0RnNnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexNVczPjBpPkK3NVE4OjZyPD;hQi=>
U373-MAGI Ml\USpVv[3Srb36gZZN{[Xl? M4m4NFIxOCC3TR?= MUGyJIhzew>? NGftcJhT\WS3Y4Tpc44hcW5iZFPUVEBt\X[nbDDpckBpfW2jbjDVN|c{NU2DR1mgZ4VtdHNiYYSgNlAxKHWPIIDy[Ylv[3WkYYTl[EBnd3JiMjDodpMh\m:ubH;3[YQh[nliNT3hfoEu\ENiYXTkbZRqd25ibXXhd5Vz\WRiYX\0[ZIhPCCqcoOgZpkhVENvTWOvUXMh[W6jbInzbZMhemWuYYTpeoUhfG9iNT3hfoEu\EN? M3TLVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUG3NlYxLz5{N{GxO|I3ODxxYU6=
U373-MAGI NYXrUJdKSW62aY\pdoFtKGG|c3H5 MWK1NEB2VQ>? MkHjNkB1dyB5MjDodpM> MX3Qc5RmdnSrYYTpc44hd2ZiNT3BfoEu\ENvaX7keYNm\CCjboTpeolz[WxiYXP0bZZqfHliYXfhbY5{fCCYU2[tS{Bxe2W3ZH;0fZBm\CCKSW[tNUBPVDRvMzDpcoZm[3SnZDDpckBpfW2jbjDVN|c{NU2DR1mgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKFV3LXfh[{Bt\X[nbDDheEA2OCC3TTDh[pRmeiB{IITvJFczKGi{czDifUByWEOUIH3leIhw\A>? MkfhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzMUeyOlAoRjJ5MUG3NlYxRC:jPh?=
U373-MAGI M2jTPGFvfGm4aYLhcEBie3OjeR?= NWe0XWFCPTBidV2= M17CZVIhfG9iN{KgbJJ{ NVLpcJJ3WG:2ZX70bYF1cW:wIH;mJFUuSXqjLVOtbY5lfWOnZDDhcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDWV3YuTyCyc3X1[I91gXCnZDDITXYuOSCQTEStN{Bqdm[nY4Tl[EBqdiCqdX3hckBWOzd|LV3BS2kh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHfh[{Bt\X[nbDDheEA2OCC3TTDh[pRmeiB{IITvJFczKGi{czDifUByWEOUIH3leIhw\A>? MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzFzN{K2NEc,OjdzMUeyOlA9N2F-
U373-MAGI NW\x[GV[SW62aY\pdoFtKGG|c3H5 MUW1NEB2VQ>? MV:yJJRwKDd{IHjydy=> MkHuVI91\W62aXH0bY9vKG:oIEWtRZpiNWSFLXnu[JVk\WRiYX70bZZqemGuIHHjeIl3cXS7IHHnZYlve3RiVmPWMWcheHOndXTveJlx\WRiSFnWMVEhVkx2LUOgbY5n\WO2ZXSgbY4hcHWvYX6gWVM4Oy2PQVfJJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCpYXegcIV3\WxiYYSgOVAhfU1iYX\0[ZIhOiC2bzC3NkBpenNiYomgdXBEWiCvZYToc4Q> MorWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzMUeyOlAoRjJ5MUG3NlYxRC:jPh?=
U373-MAGI NY[1XHRpSW62aY\pdoFtKGG|c3H5 NIPqU|U2OCC3TR?= M{jzeFYhcHK| M1\s[GFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JHZUXi2JIIDz[ZVld3S7cHXkJGhKXi1zIF7MOE0{KGmwZnXjeIVlKGmwIHj1cYFvKFV|N{OtUWFIUSClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gWVUu\2GpIHzleoVtKGG2IEWwJJVOKG2nYYP1doVlKGG2IE[gbJJ{KHCxc4SgbY5n\WO2aX;uJIJ6KHGSQ2KgcYV1cG:m NFq5boI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{GxO|I3OCd-MkexNVczPjB:L3G+
DH5aplha Mni4SpVv[3Srb36gZZN{[Xl? MkDVNkBucW6| Mki2TY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCFWWCxRVEh\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliRFi1ZZBtcGFiY3XscJMh[XO|ZYPz[YQh[XNiTz3k[YV1cHmuYYTpc44hd2ZiZYToc5h6emW|b4L1[olvKGmwIIDy[ZNmdmOnIH;mJG5CTFCKIH3lZZN2emWmIHHmeIVzKDJibXnud{BjgSC|cHXjeJJw\my3b4LvcYV1emmlIH3leIhw\CxiSVO1NF0{PM7:TT6= M2f1eVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5Mk[1NlU6Lz5{N{K2OVI2QTxxYU6=
MDAH 2774 NVriNY11S3m2b4TvfIlkcXS7IHHzd4F6 NIP3[Vk4OiCqcoO= MnzOR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCUCB{N{e0JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFPEOVA:OTRwNt88UU4> M33KdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NUG1N|E6Lz5{N{WxOVMyQTxxYU6=
T24 MVjDfZRwfG:6aXPpeJkh[XO|YYm= NULwVmVIPzJiaILz M3TQSmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHQzPCClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBETDVyPUKyMlfPxE1w NI\afYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{WxOVMyQSd-Mke1NVU{OTl:L3G+
MDAH 2774 NYroblAxS3m2b4TvfIlkcXS7IHHzd4F6 NE\Yb2Q1QCCqcoO= MXTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGFJKDJ5N{SgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiQ1S1NF0zO87:TT6= MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzVzNUOxPUc,Ojd3MUWzNVk9N2F-
DU145 MUDDfZRwfG:6aXPpeJkh[XO|YYm= NFrlV5E4OiCqcoO= MVzDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDEWVE1PSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBETDVyPUK3MlnPxE1w NV7PTndbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke1NVU{OTlpPkK3OVE2OzF7PD;hQi=>
MHRF M1[1cWN6fG:2b4jpZ4l1gSCjc4PhfS=> MWW3NkBpenN? NFjIU3lEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOUFKIIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEOGNUC9N|LPxE1w MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzVzNUOxPUc,Ojd3MUWzNVk9N2F-
MDAH 2774 NHzRNlREgXSxdH;4bYNqfHliYYPzZZk> Mli2NlQhcHK| NEDHXWZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEGKIEK3O|Qh[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeTygR2Q2OD1|Nj65{txONg>? NVzBOGNURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke1NVU{OTlpPkK3OVE2OzF7PD;hQi=>
HCT116 MUjDfZRwfG:6aXPpeJkh[XO|YYm= Mn;kNVAxKHWP MXi0PEBpenN? MlO0R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVOTF4IHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwh\3Kxd4ToJIlvcGmkaYTpc44h[XRiMUCwJJVOKGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZk> NWP3NXlGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke4PFc5PDNpPkK3PFg4QDR|PD;hQi=>
Bel7402 NWDLVVRiS3m2b4TvfIlkcXS7IHHzd4F6 MlmxNVAxKHWP NHPJRXg1QCCqcoO= M2m2O2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGJmdDd2MEKgZ4VtdHNiYYPz[ZN{\WRiYYOgZ4VtdCCpcn;3eIghcW6qaXLpeIlwdiCjdDCxNFAhfU1iYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigQ>? M4nXXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5OEi3PFQ{Lz5{N{i4O|g1OzxxYU6=
HepG2 M1u1W2Z2dmO2aX;uJIF{e2G7 NFfIOYM2KGi{cx?= MmrMRYN1cX[jdHnvckBw\iCQcn[yJEh2dmuwb4fuJI9zcWerbjmg[ZhxemW|c3XkJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFUhcHK|IHL5JGFTTS2mcnn2[Y4hdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxRTVwNN88UU4> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDF{NkS0NEc,OjhzMk[0OFA9N2F-
HEK293 M1\rdWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NV;TcmZsOiCmYYnz NGPJVWdIem:5dHigbY5pcWKrdHnvckBw\iCKRVuyPVMh[2WubIOgZYZ1\XJiMjDkZZl{KGK7IF3UWEBie3OjeTygTWM2OD1zN988UU4> NF\Gc|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OESwPFIzPCd-Mki0NFgzOjR:L3G+
MCF7 MXLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NF7WXXEzKGSjeYO= NF[2[|JIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFIh\GG7czDifUBOXFRiYYPzZZktKEmFNUC9N|TPxE1w NIf2Tpc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OESwPFIzPCd-Mki0NFgzOjR:L3G+
Ramos M4XXU2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoC2NkBl[Xm| NWj2ZpJWT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hWmGvb4OgZ4VtdHNiYX\0[ZIhOiCmYYnzJIJ6KE2WVDDhd5NigSxiSVO1NF0{P87:TT6= NEfIdFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OESwPFIzPCd-Mki0NFgzOjR:L3G+
ALL5 NGjZeGJEgXSxdH;4bYNqfHliYYPzZZk> M3znW|EzOCCqcoO= M2nvTmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGFNVDViY3XscJMh[W[2ZYKgNVIxKGi{czDifUBOXFRibXX0bI9lNCCLQ{WwQVExNjYQvF2u NHrMT|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OES5PVc{Oid-Mki0PVk4OzJ:L3G+
HEK293 NXLGfoVNTnWwY4Tpc44h[XO|YYm= MXvJcohq[mm2aX;uJI9nKGi3bXHuJHRTWEFzIHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4hSUmWQz3pcoR2[2WmIHP1dpJmdnRicnXzdI9ve2ViYomge4hwdGViY3XscEBx[XSlaDDjcIFueCCvZYToc4QtKEmFNUC9NE44Pc7:TT6= NFzkcVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW3OlYyPyd-Mki1O|Y3OTd:L3G+
LN229 MU\DfZRwfG:6aXPpeJkh[XO|YYm= MlTzOFghcHK| NH;JVFNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBNVjJ{OTDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG|ZTDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTFzzszNMi=> NVXrXnRVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2OVQzPjVpPkK4OlU1OjZ3PD;hQi=>
NCI-H460 MmrjR5l1d3SxeHnjbZR6KGG|c3H5 MYe0PEBpenN? NHfkXpREgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBPS0lvSES2NEBk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiClZXzsJJZq[WKrbHn0fUBi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUGy{txONg>? M2ra[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NkW0NlY2Lz5{OE[1OFI3PTxxYU6=
MML1 MV7DfZRwfG:6aXPpeJkh[XO|YYm= NYjIRWZPPDhiaILz NVG3UY5SS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVU2OMTDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG|ZTDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTF{zszNMi=> NIPje4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[1OFI3PSd-Mki2OVQzPjV:L3G+
BxPC3 M{jJV2N6fG:2b4jpZ4l1gSCjc4PhfS=> M33tZlQ5KGi{cx?= M4PLcWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGJ5WEN|IHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OTUQvF2u Mnv1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4NUSyOlUoRjJ6NkW0NlY2RC:jPh?=
SK-MEL-2 NGnWeJdEgXSxdH;4bYNqfHliYYPzZZk> MnzjOFghcHK| MVzDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT{1OTUxvMjDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG|ZTDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTF2zszNMi=> M2m1N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NkW0NlY2Lz5{OE[1OFI3PTxxYU6=
MDA-MB-231 MkPrR5l1d3SxeHnjbZR6KGG|c3H5 NELOcmw1QCCqcoO= NYTBZnN4S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVE2|ryPLh?= MoKzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4NUSyOlUoRjJ6NkW0NlY2RC:jPh?=
A549 M4DrTGN6fG:2b4jpZ4l1gSCjc4PhfS=> MnKxOFghcHK| Mn3WR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVE3|ryPLh?= M4LYOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NkW0NlY2Lz5{OE[1OFI3PTxxYU6=
U87 MlOxR5l1d3SxeHnjbZR6KGG|c3H5 NV3nWZZNPDhiaILz NHrDfVZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBWQDdiY3XscJMh[XO|ZYPz[YQh[XNiZHXjdoVie2ViaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0zOM7:TT6= NHHMSIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[1OFI3PSd-Mki2OVQzPjV:L3G+
T47D M4X4bWN6fG:2b4jpZ4l1gSCjc4PhfS=> NXnwbHBmPDhiaILz MVLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDUOFdFKGOnbHzzJIF{e2W|c3XkJIF{KGSnY4LlZZNmKGmwIHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NlXPxE1w MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDZ3NEK2OUc,Ojh4NUSyOlU9N2F-
HT-29 M{ju[WN6fG:2b4jpZ4l1gSCjc4PhfS=> M4rVPFQ5KGi{cx?= MXnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIWE0zQSClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVI4|ryPLh?= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDZ3NEK2OUc,Ojh4NUSyOlU9N2F-
COLO201 M{HNb2N6fG:2b4jpZ4l1gSCjc4PhfS=> NV\Kb4JCPDhiaILz NIC4U2ZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBEV0yRMkCxJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;M{hOwG0v NXfNZWxFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2OVQzPjVpPkK4OlU1OjZ3PD;hQi=>
PANC1 M2rJZWN6fG:2b4jpZ4l1gSCjc4PhfS=> MlX6OFghcHK| MnPKR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVGFPSzFiY3XscJMh[XO|ZYPz[YQh[XNiZHXjdoVie2ViaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0{Qc7:TT6= NV\Rd5c{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2OVQzPjVpPkK4OlU1OjZ3PD;hQi=>
A549 MonxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MoOxS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gRVU1QSClZXzsd{whUUN3ME2zNO69VS5? M1HGd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OEG0N|c1Lz5{OEixOFM4PDxxYU6=
BV2 MXrBcpRqdmW3cn;pcoZt[W2vYYTvdpkh[XO|YYm= MmHoNlQhcHK| NGPQSplCdnSrbnX1do9qdm[uYX3tZZRwenliYXP0bZZqfHliaX6gcY92e2ViQm[yJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgUm8heHKxZIXjeIlwdiCjZoTldkAzPCCqcoOgbY4heHKnc3XuZ4Uhd2ZiTGDTJIJ6KEe{aXXzd{Bz\WGldHnvckBj[XOnZDDhd5NigSxiSVO1NF0yQC54Nt88UU4> NYDCU2U4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5NVE5OTdpPkK4PVEyQDF5PD;hQi=>
A375 NFL0W4lHfW6ldHnvckBie3OjeR?= MmfxNlUhfU1? MkDMNlQhcHK| MV3Jcohq[mm2aX;uJI9nKGGucHjhMW1UUC2rbnT1Z4VlKFSUUD2xJIV5eHKnc4Ppc44hcW5iaIXtZY4hSTN5NTDj[YxteyCjdDCyOUB2VSCjZoTldkAzPCCqcoOgZpkhX2W|dHXyckBjdG:2IH3leIhw\A>? Ml3JQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzNUCzN|MoRjJ7MUWwN|M{RC:jPh?=
A375 MmfwSpVv[3Srb36gZZN{[Xl? NIfwR4gzPSC3TR?= NYnKNnExOjRiaILz M3n4TWlvcGmkaYTpc44hd2ZiYXzwbIEuVVOKLXnu[JVk\WRiVGLQMVIh\XiycnXzd4lwdiCrbjDoeY1idiCDM{e1JINmdGy|IHH0JFI2KHWPIHHmeIVzKDJ2IHjyd{BjgSCZZYP0[ZJvKGKub4SgcYV1cG:m M{LEb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUWwN|M{Lz5{OUG1NFM{OzxxYU6=
A375 M1XVOWZ2dmO2aX;uJIF{e2G7 NXmwXZlsOjVidV2= MWm0JIhzew>? NFWzSmNKdmirYnn0bY9vKG:oIHHsdIhiNU2VSD3pcoR2[2WmIILlZYN1cX[nIH;4fYdmdiC|cHXjbYV{KGenbnXyZZRqd25iaX6gbJVu[W5iQUO3OUBk\WyuczDheEAzPSC3TTDh[pRmeiB2IHjyd{BjgSCGQ1\ERUB{fGGrbnnu[{1j[XOnZDDmcJVwemW|Y3XuZ4Uh[XO|YYm= MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF3MEOzN{c,OjlzNUCzN|M9N2F-
HEK293T MUjDfZRwfG:6aXPpeJkh[XO|YYm= MVqyOEBpenN? M1\iOWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEiHS{K5N3Qh[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeTygR2M2OD1{Mj63Oe69VS5? NXHkeHdORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxO|IxPzlpPkK5NVczODd7PD;hQi=>
3T3L1 NHX2XHJHfW6ldHnvckBie3OjeR?= MYGxJJRwKDFyIIXN NYD5ZYpyPiCmYYnz NVfDd2d7UW6qaXLpeIlwdiCxZjD0dolodHmlZYLp[IUh[WOldX31cIF1cW:wIHnuJI1wfXOnIEPUN2wyKGOnbHzzJIF1KDFidH:gNVAhfU1iYX\0[ZIhPiCmYYnzJIJ6KE:UTzDzeIFqdmmwZz3iZZNm\CCvaXPyc5Nkd3CrYzDt[ZRpd2R? M1vjb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUeyNFc6Lz5{OUG3NlA4QTxxYU6=
RAW264.7 MonYSpVv[3Srb36gZZN{[Xl? NES3N3AyOCC3TR?= NWi5SJloOjRiaILz MnzZTY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDUUmYu[WyyaHGgdJJw\HWldHnvckBqdiC{YYSgVmFYOjZ2LkegZ4VtdHNiYYSgNVAhfU1iYX\0[ZIhOjRiaILzJIJ6KEWOSWPB M4LybVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEC3PVQ1Lz5{OUSwO|k1PDxxYU6=
RAW264.7 MVrGeY5kfGmxbjDhd5NigQ>? NEH6XlgyOCC3TR?= NVTNTXN[OjRiaILz MnrrTY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDJUE03KHC{b3T1Z5Rqd25iaX6gdoF1KFKDV{K2OE44KGOnbHzzJIF1KDFyIIXNJIFnfGW{IEK0JIhzeyCkeTDFUGlUSQ>? M3\jOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEC3PVQ1Lz5{OUSwO|k1PDxxYU6=
SK-N-MC MWTxTHRUKGG|c3H5 NGKwV3RyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1OSyClZXzsdy=> Mm\YQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
BT-37 MkKxdWhVWyCjc4PhfS=> M3zDeJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCEVD2zO{Bk\Wyucx?= M2PDOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
BV2 NXLUZ2h[SW62aX7leZJwcW6obHHtcYF1d3K7IHHzd4F6 NV;HZmZUOjRiaILz NYi1enI2SW62aX7leZJwcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJI1wfXOnIFLWNkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJGlNNTGkZYThJJBzd2S3Y4Tpc44h[W[2ZYKgNlQhcHK|IHL5JGVNUVODLDDJR|UxRThwN988UU4> NVj3dZVpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0PVk1QDVpPkK5OFk6PDh3PD;hQi=>
BV2 Mn34RY51cW6ndYLvbY5ndGGvbXH0c5J6KGG|c3H5 MVqyOEBpenN? M2S2T2FvfGmwZYXyc4lv\myjbX3heI9zgSCjY4Tpeol1gSCrbjDtc5V{\SCEVkKgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDUUmZidHCqYTDwdo9lfWO2aX;uJIFnfGW{IEK0JIhzeyCkeTDFUGlUSSxiSVO1NF06NjYQvF2u MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR7OUS4OUc,Ojl2OUm0PFU9N2F-
BV2 M1;iRmFvfGmwZYXyc4lv\myjbX3heI9zgSCjc4PhfS=> MkHJN|AhdWmwcx?= NGrJSpdCdnSrbnX1do9qdm[uYX3tZZRwenliYXP0bZZqfHliaX6gcY92e2ViQm[yJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgUm8heHKxZIXjeIlwdiCycnX0doVifGWmIH\vdkA{OCCvaX7zJIZwdGyxd3XkJIJ6KEySUzDzeIlufWyjdHnvckBidmRibXXhd5Vz\WRiYX\0[ZIhOjRiaILzJIJ6KEe{aXXzd{Bie3OjeTygTWM2OD1zMT6x{txONg>? MkDIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2OUm0PFUoRjJ7NEm5OFg2RC:jPh?=
J774 NGjKVVBCdnSraX7mcIFudWG2b4L5JIF{e2G7 MnTKNlQhcHK| M2L0UWFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgTlc4PCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gUHBUNWmwZIXj[YQhUUx4IHX4dJJme3Orb36gZYZ1\XJiMkSgbJJ{KGK7IFXMTXNCNCCLQ{WwQVYvQM7:TT6= M4L6TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{K2OlgxLz5{OUeyOlY5ODxxYU6=
J774 NFr5WlBCdnSraX7mcIFudWG2b4L5JIF{e2G7 M{Hod|I1KGi{cx?= M{HZ[GFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgTlc4PCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gUHBUNWmwZIXj[YQhdmm2cnnjJI95cWSnIIDyc4R2[3Srb36gZYZ1\XJiMkSgbJJ{KGK7IFfybYV{eyCjc4PhfUwhUUN3ME2xNk4yPc7:TT6= MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd{Nk[4NEc,Ojl5Mk[2PFA9N2F-
J774 MoTVRY51cWmwZnzhcY1ifG:{eTDhd5NigQ>? NFrhOpIzPCCqcoO= NHrPSpdCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKG2xdYPlJGo4PzRiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJGxRWy2rbnT1Z4VlKE2FUEGg[ZhxemW|c3nvckBi\nSncjCyOEBpenNiYomgSWxKW0FuIFnDOVA:OTNwNt88UU4> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd{Nk[4NEc,Ojl5Mk[2PFA9N2F-
RAW 264.7 NXm1SYpXTnWwY4Tpc44h[XO|YYm= MU[yOEBpenN? M13wS2lvcGmkaYTpc44hd2ZiTl\rZZBx[UJiaX6gcY92e2ViUlHXJFI3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBNWFNiaX7keYNm\CCQTzDwdo9lfWO2aX;uJIFnfGW{IEK0JIhzeyCrbjDwdoV{\W6lZTDv[kBQSkiVIHL5JGdzcWW|czDhd5NigSxiSVO1NF0yOc7:TT6= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODB3M{e4N{c,OzByNUO3PFM9N2F-
RAW 264.7 NW\mZZhLTnWwY4Tpc44h[XO|YYm= MYWyOEBpenN? MXvJcohq[mm2aX;uJI9nKE6Ia3HwdIFDKGmwIH3veZNmKFKDVzCyOlQvPyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gUHBUKGmwZIXj[YQhVk9icILv[JVkfGmxbjDh[pRmeiB{NDDodpMh[nliR4Lp[ZN{KGG|c3H5MEBKSzVyPUGzMlHPxE1w M3[1OFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMEWzO|g{Lz5|MEC1N|c5OzxxYU6=
MCF7 MV;BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MnLwO|IhcHK| NXvUcFAzSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MkKuPe69VS5? M4nHW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMEWzO|g{Lz5|MEC1N|c5OzxxYU6=
MCF7 NYf6NFhCSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M4L0VVczKGi{cx?= MVHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiYX\0[ZIhPzJiaILzJIlvKHC{ZYPlcoNmKG:oIF;CTHMh[nliTWTUJIF{e2G7LDDJR|UxRTJ3LkVOwG0v MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODB3M{e4N{c,OzByNUO3PFM9N2F-
BV2 NFvRWmpCdnSrbnX1do9qdm[uYX3tZZRwenliYYPzZZk> NUDmeYF5OTBidH:gN|AhfU1? NIHISFkzPCCqcoO= NUjnTWM5SW62aX7leZJwcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJI1wfXOnIFLWNkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJG5QKHC{b3T1Z5Rqd25iYYSgNVAhfG9iM{CgeW0h[W[2ZYKgNlQhcHK|IHL5JGdzcWW|czDhd5NigQ>? NGnvW2Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEC5OlY2OCd-M{CwPVY3PTB:L3G+
RAW264.7 MlvpRY51cWmwZnzhcY1ifG:{eTDhd5NigQ>? Mn3tNUBpeg>? MVrBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhVFCVL1nGUodidW2jLXnu[JVk\WRiTl:gdJJw\HWldHnvckBjgSCvZXHzeZJqdmdiTl:gcIV3\Wy|IHnuZ5Vj[XSnZDD3bZRpKEySUz;JSm5o[W2vYTDmc5IhOSCqcjDmc4xtd3enZDDifUBkd22yb4Xu[EBi\GSrdHnvckBu\WG|dYLl[EBi\nSncjC0PEBpenNiYomgS5Jq\XO|IHHzd4F6KCiUdnKgQUA2PSBtLz2gNk4tKEGldHn2bZR6RTR2LkZOwG0v MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODF7M{m0NUc,OzBzOUO5OFE9N2F-
T cells MWfBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NF;YdFg2OCC3TR?= M{fucFEhcHJ? NV;qZZRySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBEd25vQT3pcoR2[2WmIGSgZ4VtdHNiaYPvcIF1\WRiZoLvcUB{eGynbn;jfZRmeyCxZjDDOVcwSkx4IH3veZNmKGG2IEWwJJVOKGmwY4XiZZRm\CC5aYToJGNwdi2DIH\vdkAyKGi{IH\vcIxwf2WmIHL5JINwdXCxdX7kJIFl\Gm2aX;uJI1m[XO3cnXkJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYm= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODF7M{m0NUc,OzBzOUO5OFE9N2F-
B cells M17KWmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NXvIVo5FPTBidV2= MnPNNUBpeg>? NHXLcVRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGxRWy2rbnT1Z4VlKEJiY3XscJMhcXOxbHH0[YQh\nKxbTDzdIxmdm:leYTld{Bw\iCFNUevRmw3KG2xdYPlJIF1KDVyIIXNJIlv[3WkYYTl[EB4cXSqIFzQV{Bnd3JiMTDodkBnd2yub4fl[EBjgSClb33wc5Vv\CCjZHTpeIlwdiCvZXHzeZJm\CCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6 NIXhT3Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEG5N|k1OSd-M{CxPVM6PDF:L3G+
RAW264.7 MVvJcY12dm:vb3T1cIF1d3K7IHHzd4F6 M1TKRlUxKHWP NVq5bm9yOSCqch?= MYDJcY12dm:vb3T1cIF1d3K7IHHjeIl3cXS7IHnuJGxRWy2|dHnteYxifGWmIH3veZNmKFKDV{K2OE44KGOnbHzzJIF{e2W|c3XkJIF{KGSnY4LlZZNmKGmwIFPEPFAh\XiycnXzd4lwdiCjdDC1NEB2VSCrbnP1ZoF1\WRid3n0bEBNWFNiZn;yJFEhcHJiZn;scI94\WRiYomgZ49ueG:3bnSgZYRlcXSrb36gcYVie3W{ZXSgZYZ1\XJiMkSgbJJ{KGK7IH\sc5ch[3m2b33leJJ6 NI\BVIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEG5N|k1OSd-M{CxPVM6PDF:L3G+
RAW264.7 MlzGTY1ufW6xbX;keYxifG:{eTDhd5NigQ>? M1e0flUxKHWP MWGxJIhz MkHnTY1ufW6xbX;keYxifG:{eTDhZ5Rqfmm2eTDpckBNWFNvc4TpcZVt[XSnZDDtc5V{\SCUQWeyOlQvPyClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDDSFg3KGW6cILld5Nqd25iYYSgOVAhfU1iaX7jeYJifGWmIIfpeIghVFCVIH\vdkAyKGi{IH\vcIxwf2WmIHL5JINwdXCxdX7kJIFl\Gm2aX;uJI1m[XO3cnXkJIFnfGW{IEK0JIhzeyCkeTDmcI94KGO7dH;t[ZRzgQ>? M4myU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMUmzPVQyLz5|MEG5N|k1OTxxYU6=
HBZY-1 NUXBZ2lkTnWwY4Tpc44h[XO|YYm= MVi2JJVO NGDpWYRC[3SrdnH0bY9vKG:oIF7y[lIhcW5icnH0JGhDYllvMTDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBPWU9vMTDs[ZZmdCCjdDC2JJVOKGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> MoHUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB2MEi3OFkoRjNyNEC4O|Q6RC:jPh?=
HBZY-1 Mni2SpVv[3Srb36gZZN{[Xl? NYH6UYp3PiC3TR?= NEDoVGdC[3SrdnH0bY9vKG:oIF7y[lIhcW5icnH0JGhDYllvMTDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBJVy1zIHzleoVtKGG2IE[geW0h[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz NIfXSJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MESwPFc1QSd-M{C0NFg4PDl:L3G+
HBZY-1 NYDyeoFSTnWwY4Tpc44h[XO|YYm= NUPXe|ZZPiC3TR?= NYTyd3JtSWO2aY\heIlwdiCxZjDOdoYzKGmwIILheEBJSlq\LUGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hT0OOTTDs[ZZmdCCjdDC2JJVOKGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> MlG4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB2MEi3OFkoRjNyNEC4O|Q6RC:jPh?=
HBZY-1 MUHGeY5kfGmxbjDhd5NigQ>? MWO2JJVO MW\BZ5RqfmG2aX;uJI9nKE6{ZkKgbY4hemG2IFjCXnkuOSClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDHR2xEKGyndnXsJIF1KDZidV2gZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODRyOEe0PUc,OzB2MEi3OFk9N2F-
HBZY-1 NY\0RZdrTnWwY4Tpc44h[XO|YYm= MWS2JJVO NHnke3UzPCCqcoO= NVnxe5R7SWO2aY\heIlwdiCxZjDOdoYzKGmwIILheEBJSlq\LUGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hVlGRLUGgcXJPSSCneIDy[ZN{cW:wIHH0JFYhfU1iYX\0[ZIhOjRiaILzJIJ6KFKWLWDDVkBidmGueYPpdy=> NWf5NWdCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C0NFg4PDlpPkOwOFA5PzR7PD;hQi=>
HBZY-1 MYTGeY5kfGmxbjDhd5NigQ>? NWfTZ401PiC3TR?= Mn3SNlQhcHK| MoDBRYN1cX[jdHnvckBw\iCQcn[yJIlvKHKjdDDIRnp[NTFiY3XscJMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gTG8uOSCvUl7BJIV5eHKnc4Ppc44h[XRiNjD1UUBi\nSncjCyOEBpenNiYomgVnQuWEOUIHHuZYx6e2m| NH7ufHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MESwPFc1QSd-M{C0NFg4PDl:L3G+
HBZY-1 NEXQRnFHfW6ldHnvckBie3OjeR?= NX22ZWJSPiC3TR?= NWn0[WdSOjRiaILz NWGzVZlvSWO2aY\heIlwdiCxZjDOdoYzKGmwIILheEBJSlq\LUGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hT0OOTTDtVm5CKGW6cILld5Nqd25iYYSgOkB2VSCjZoTldkAzPCCqcoOgZpkhWlRvUFPSJIFv[Wy7c3nz NGW5PG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MESwPFc1QSd-M{C0NFg4PDl:L3G+
HBZY-1 NWPNbGdWTnWwY4Tpc44h[XO|YYm= NHfEOHc3KHWP NE\RWogzPCCqcoO= MlP2RYN1cX[jdHnvckBw\iCQcn[yJIlvKHKjdDDIRnp[NTFiY3XscJMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gS2NNSyCvUl7BJIV5eHKnc4Ppc44h[XRiNjD1UUBi\nSncjCyOEBpenNiYomgVnQuWEOUIHHuZYx6e2m| MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODRyOEe0PUc,OzB2MEi3OFk9N2F-
BV2 MlX5RY51cW6ndYLvbY5ndGGvbXH0c5J6KGG|c3H5 MY[zNEBucW6| NVLLSWlDOjRiaILz MWTBcpRqdmW3cn;pcoZt[W2vYYTvdpkh[WO2aY\peJkhcW5ibX;1d4UhSlZ{IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhVFCVLXnu[JVk\WRibnn0dolkKG:6aXTlJJBzd2S3Y4Tpc44heHKndILlZZRm\CCob4KgN|AhdWmwczDmc4xtd3enZDDifUBNWFNvc4TpcZVt[XSrb36g[o9zKDJ2IHjyd{BjgSCJcnnld5Mh[XO|YYmsJGlEPTB;MUGuNe69VS5? M{TUTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyN{exOlAyLz5|MEe3NVYxOTxxYU6=
MCF7 M{DCTGFvfGmxeHnkZY51KGG|c3H5 MmPkNVAxKHWP Ml7lNlQhcHK| NUfzWVlLSW62aX;4bYRidnRiYXP0bZZqfHliaX6gbJVu[W5iTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiC{ZXHjeIl3\SCxeInn[Y4he3CnY3nld{Bo\W6ncnH0bY9vKGG2IEGwNEB2VSCrbnP1ZoF1\WRiZn;yJFI1KGi{czDifUBE\WyuUl;YJIdz\WWwL1jv[YNpe3RiM{OzOFIhe3SjaX7pcoch[mG|ZXSgZZN{[Xl? M4XHdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyOUKxO|U5Lz5|MEmyNVc2QDxxYU6=
RAW264.7 NXTmU3A1SW62aXnu[oxidW2jdH;yfUBie3OjeR?= Mn3lNVAhfU1? NFzre3cyKGi{ M{D5bmFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDOU{Bxem:mdXP0bY9vKGG2IEGwJJVOKHC{ZYTy[YF1\WRiZn;yJFEhcHJiZn;scI94\WRiYomgUHBUKHO2aX31cIF1cW:wIHHu[EBu\WG|dYLl[EBi\nSncjCyOEBpenNiYomgS5Jq\XO|IILlZYdmdnRiYnHz[YQh[XO|YYm= MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTB5N{m5O{c,OzFyN{e5PVc9N2F-
HBZY-1 NYj6R3U5TnWwY4Tpc44h[XO|YYm= NI\ZeY5DcW6maX7nJIFn\mmwaYT5JJRwKEunYYCxJIlvKHKjdDDIRnp[NTFiY3XscJMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJG5z\jJiboXjcIVieiC2cnHud4xw[2G2aX;uJIJ6KG2nYYP1dolv\yCrbnPy[YF{\SCrbjDyZZRqdyCxZjDueYNt\WG{IITvJIN6fG:|b3zpZ{BPemZ{IHzleoVtKGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTNyMUW2OEc,OzF|MEG1OlQ9N2F-
HBZY-1 NWW0cG1qTnWwY4Tpc44h[XO|YYm= M3;4S2JqdmSrbnegZYZncW6rdImgeI8hU2WjcEGgbY4hemG2IFjCXnkuOSClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gT4VieDFxToLmNkB{cWewYXzpcoch[nlibXXhd5VzcW6pIHnuZ5Jm[XOnIHnuJG5SVy1zIIDyc5RmcW5iZYjwdoV{e2mxbjDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? MmPtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF|MEG1OlQoRjNzM{CxOVY1RC:jPh?=
HBZY-1 M{jkbmZ2dmO2aX;uJIF{e2G7 MYDCbY5lcW6pIHHm[olvcXS7IITvJGtm[XBzIHnuJJJifCCKQmrZMVEh[2WubIOgZZN{\XO|ZXSgZZMhcW6mdXP0bY9vKG:oIFvlZZAyN06{ZkKgd4lodmGuaX7nJIJ6KG2nYYP1dolv\yCrbnPy[YF{\SCrbjDIU{0yKHC{b4TlbY4h\XiycnXzd4lwdiCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> M1r5e|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzM{CxOVY1Lz5|MUOwNVU3PDxxYU6=
HBZY-1 NEnWWWpHfW6ldHnvckBie3OjeR?= M1LrXGJqdmSrbnegZYZncW6rdImgeI8hU2WjcEGgbY4hemG2IFjCXnkuOSClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gT4VieDFxToLmNkB{cWewYXzpcoch[nlibXXhd5VzcW6pIHnuZ5Jm[XOnIHnuJGdEVE1icILveIVqdiCneIDy[ZN{cW:wIHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTNyMUW2OEc,OzF|MEG1OlQ9N2F-
HBZY-1 MWDGeY5kfGmxbjDhd5NigQ>? NVHvSI1VSmmwZHnu[{Bi\m[rbnn0fUB1dyCNZXHwNUBqdiC{YYSgTGJbYS1zIHPlcIx{KGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBM\WGyMT;OdoYzKHOrZ37hcIlv\yCkeTDt[YF{fXKrbnegbY5kemWjc3WgbY4hT0OOQzDwdo91\WmwIHX4dJJme3Orb36gZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTNyMUW2OEc,OzF|MEG1OlQ9N2F-
HBZY-1 NH\Yd3NHfW6ldHnvckBie3OjeR?= MWS2JJVO NEDjdJRDcW6maX7nJIFn\mmwaYT5JJRwKEunYYCxJIlvKHKjdDDIRnp[NTFiY3XscJMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJGtm[XBzL17y[lIhe2mpbnHsbY5oKGK7IH3lZZN2emmwZzDpcoNz\WG|ZTDpckBPWU9vMTDtVm5CKGW6cILld5Nqd25iYYSgOkB2VSCkeTDSWE1RS1JiYX7hcJl{cXN? NGrDfZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUOwNVU3PCd-M{GzNFE2PjR:L3G+
HBZY-1 NEHIPGVHfW6ldHnvckBie3OjeR?= NHTnVXg3KHWP NV[z[|lGSmmwZHnu[{Bi\m[rbnn0fUB1dyCNZXHwNUBqdiC{YYSgTGJbYS1zIHPlcIx{KGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBM\WGyMT;OdoYzKHOrZ37hcIlv\yCkeTDt[YF{fXKrbnegbY5kemWjc3WgbY4hUE9vMTDtVm5CKGW6cILld5Nqd25iYYSgOkB2VSCkeTDSWE1RS1JiYX7hcJl{cXN? NFnnWpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUOwNVU3PCd-M{GzNFE2PjR:L3G+
HBZY-1 M1\GN2Z2dmO2aX;uJIF{e2G7 M3rJR|YhfU1? NITuUoNDcW6maX7nJIFn\mmwaYT5JJRwKEunYYCxJIlvKHKjdDDIRnp[NTFiY3XscJMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJGtm[XBzL17y[lIhe2mpbnHsbY5oKGK7IH3lZZN2emmwZzDpcoNz\WG|ZTDpckBIS0yPIH3SUmEh\XiycnXzd4lwdiCjdDC2JJVOKGK7IGLUMXBEWiCjbnHsfZNqew>? NV7JZ5hTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{GzNFE2PjRpPkOxN|AyPTZ2PD;hQi=>
HBZY-1 MkTCSpVv[3Srb36gZZN{[Xl? M4\3WVYhfU1? MoLTRolv\GmwZzDh[oZqdmm2eTD0c{BM\WGyMTDpckBz[XRiSFLaXU0yKGOnbHzzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDL[YFxOS:Qcn[yJJNq\26jbHnu[{BjgSCvZXHzeZJqdmdiaX7jdoVie2ViaX6gS2NNSyCvUl7BJIV5eHKnc4Ppc44h[XRiNjD1UUBjgSCUVD3QR3Ih[W6jbInzbZM> NYmybWdxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{GzNFE2PjRpPkOxN|AyPTZ2PD;hQi=>
RAW264.7 NGq3NoxCdnSraX7mcIFudWG2b4L5JIF{e2G7 MY[yJIhzew>? M2[0WmFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCOUGOvTWZP\2GvbXGtbY5lfWOnZDDOU{Bxem:mdXP0bY9vKGK7IH3lZZN2emmwZzDOU{Bt\X[nbDDwdoVqdmO3YnH0[YQh\m:{IEKgbJJ{KGGwZDDmc4xtd3enZDDifUBNWFNxSV\O[4FudWFiYXTkbZRqd25iYX7kJI1m[XO3cnXkJIFnfGW{IEK0JIhzeyCkeTDHdolme3NiYYPzZZktKEmFNUC9OFQvOc7:TT6= Mn;hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF|NUCxNlcoRjNzM{WwNVI4RC:jPh?=
PMNL M2DmNWZ2dmO2aX;uJIF{e2G7 MV:xOUBucW6| NUGye3lFUW6qaXLpeIlwdiCxZjC1MWxQYCCrbjDoeY1idiCSTV7MJINmdGy|IHHzd4V{e2WmIHHzJGEzOzF6Nz3zeIlufWyjdHXkJGxVSjRicILv[JVkfGmxbjDwdoVqdmO3YnH0[YQh\m:{IEG1JI1qdnNiZn;scI94\WRiYomgRVI{OTh5IHHk[Il1cW:wIHHu[EBu\WG|dYLl[EBi\nSncjCxNEBucW6|IHL5JGhRVENiYX7hcJl{cXNuIFnDOVA:PC57zszNMi=> NXPuUI5VRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{GzOVE{QTVpPkOxN|UyOzl3PD;hQi=>
H9c2 MU\BcpRqd3irZHHueEBie3OjeR?= NHLXb4Y5OCCwTTD0c{AzKHWP M4G1bFE5KGi{cx?= NWn1cJlISW62aX;4bYRidnRiYXP0bZZqfHliaX6gdoF1KEh7Y{KgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGi7cH;4bYEucW6mdXPl[EBPVyC{ZXzlZZNmKGG2IEiwJI5OKHSxIEKgeW0heHKnaX7jeYJifGWmIH\vdkAyQCCqcoOg[o9tdG:5ZXSgZpkhcW6ldXLheIVlKGmwIHj5dI95cWFiZn;yJFQ5KGi{czDifUBoemmnc4OgZZN{[Xl? NEDBblY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUO1NVM6PSd-M{GzOVE{QTV:L3G+
H9c2 NYXzPG01SW62aX;4bYRidnRiYYPzZZk> NXvyZnRGOiC3TR?= MXexPEBpenN? NXrUblBOSW62aX;4bYRidnRiYXP0bZZqfHliaX6gdoF1KEh7Y{KgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hcHmyb4jpZU1qdmS3Y3XkJJJm\HWldHnvckBw\iCPbmPPSEBmgHC{ZYPzbY9vKGG2IEKgeW0heHKnaX7jeYJifGWmIH\vdkAyQCCqcoOg[o9tdG:5ZXSgZpkhcW6ldXLheIVlKGmwIHj5dI95cWFiZn;yJFQ5KGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTN3MUO5OUc,OzF|NUGzPVU9N2F-
TOV112D MYPDfZRwfG:6aXPpeJkh[XO|YYm= MmnoPVYhcHK| NFzpPGpEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBVV1ZzMULEJINmdGy|IITyZY5{\mWldHXkJJdqfGhiZX3weJkhfmWldH;yJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCrbnP1ZoF1\WRiZn;yJFk3KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVAvPjgQvF2u MmDRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF3MUSwNVgoRjNzNUG0NFE5RC:jPh?=
TOV112D MXfDfZRwfG:6aXPpeJkh[XO|YYm= MV25OkBpenN? M1rHT2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHRQXjFzMlSgZ4VtdHNiZYjwdoV{e2mwZzDOVW8zKGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDpcoN2[mG2ZXSg[o9zKDl4IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OTIQvF2u MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTVzNECxPEc,OzF3MUSwNVg9N2F-
SKOV3 M4jUXWN6fG:2b4jpZ4l1gSCjc4PhfS=> NF\n[4g6PiCqcoO= NIDLbWlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUU0:YMzDj[YxteyC2cnHud4Zm[3SnZDD3bZRpKG6xbj30ZZJo\XSnZDD2[YN1d3JiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGOnbHygeoli[mmuaYT5JIlv[3WkYYTl[EBnd3JiOU[gbJJ{KGmwIIDy[ZNmdmOnIH;mJIRwgHmleXPsbY5mKGK7IF3UWEBie3OjeTygTWM2OD1{Od88UU4> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTVzNECxPEc,OzF3MUSwNVg9N2F-
SKOV3 MY\DfZRwfG:6aXPpeJkh[XO|YYm= NGTJfXo6PiCqcoO= MkLnR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2tQXjNiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHnuZ5Vj[XSnZDDmc5IhQTZiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0{Pi5|zszNMi=> M2jqUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNUG0NFE5Lz5|MUWxOFAyQDxxYU6=
SKOV3 M4rybWN6fG:2b4jpZ4l1gSCjc4PhfS=> NEPwdnY6PiCqcoO= NGDqWnBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUU0:YMzDj[YxteyC2cnHud4Zm[3SnZDD3bZRpKHOqUl7BJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCrbnP1ZoF1\WRiZn;yJFk3KGi{czDpckBxemW|ZX7j[UBw\iCmb4j5Z5lkdGmwZTDifUBOXFRiYYPzZZktKEmFNUC9N|kvPjgQvF2u NYPG[mdTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G1NVQxOThpPkOxOVE1ODF6PD;hQi=>
HuH7 MVHBdI9xfG:|aYOgZZN{[Xl? NFvwSZIyODBidV2= MWq0PEBpenN? M2jmbGlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iSIXIO{Bk\WyuczD0doFve2[nY4Tl[EB4cXSqIGPoV2dMOSCjc4Pld5Nm\CCjczDsZZRmKGGyb4D0c5NqeyCjdDCxNFAhfU1iaX7jeYJifGWmIH\vdkA1QCCqcoOgZpkh[W6wZYjpckBXKGGwZDC3MWFCTCC|dHHpcolv\yCkYYPl[EBie3OjeR?= NYX0dnFqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G1N|k4PzlpPkOxOVM6Pzd7PD;hQi=>
HUH7 M3zSe2Z2dmO2aX;uJIF{e2G7 NUTpOWZIOTByIIXN NFXiWoI3KGi{cx?= M4HXWGlvcGmkaYTpc44hd2ZiaX7zeYxqdi2rbnT1Z4VlKFOJS{GgbY4hcHWvYX6gTHVJPyClZXzsd{BifCBzMECgeW0hfXOrbnegdIVxfGmmZTDhd{B{fWK|dILheIUhcW6ldXLheIVlKG[xcjC2JIhzeyCkeTDzZ4lvfGmubHH0bY9vKGOxdX70[ZIhdWW2aH;k NV3JVopIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G1N|k4PzlpPkOxOVM6Pzd7PD;hQi=>
HuH7 Ml3jSpVv[3Srb36gZZN{[Xl? NYPuUXNDOTByIIXN MmXUOkBpenN? NFS0VVBKdmirYnn0bY9vKG:oIGPHT|EhcW5iaIXtZY4hUHWKNzDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hcW6|dXzpck1qdmS3Y3XkJG1FVTJicHjvd5Bpd3K7bHH0bY9vKG:wIGPldlE3PiCjdDCxNFAhfU1iaX7jeYJifGWmIH\vdkA3KGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? M13nZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNUO5O|c6Lz5|MUWzPVc4QTxxYU6=
HuH7 NWPlU|BUSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M1vvb|ExOCC3TR?= NVPvRmlSPDhiaILz NXXPZZh{SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIeWg4KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCjdDCxNFAhfU1iaX7jeYJifGWmIH\vdkA1QCCqcoOgZpkhfHK7cHHuJIJtfWViZInlJIJie2WmIHHzd4F6 M4faTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNUO5O|c6Lz5|MUWzPVc4QTxxYU6=
HuH7 NGjIU|RCeG:ydH;zbZMh[XO|YYm= MUGxNFAhfU1? NF7nSIE1QCCqcoO= MXPJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKEi3SEegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4h\WG{bImgZZBweHSxdHnjJINmdGy|IHH0JFExOCC3TTDpcoN2[mG2ZXSg[o9zKDR6IHjyd{BjgSCjbn7lfIlvKFZiYX7kJFcuSUGGIIP0ZYlvcW6pIHLhd4VlKGG|c3H5 M3nrbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNUO5O|c6Lz5|MUWzPVc4QTxxYU6=
HuH7 NFfHZVBCeG:ydH;zbZMh[XO|YYm= NXTpUWlnOTByIIXN MVq0PEBpenN? MWrJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKEi3SEegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hdGG2ZTDhdI9xfG:2aXOgZ4VtdHNiYYSgNVAxKHWPIHnuZ5Vj[XSnZDDmc5IhPDhiaILzJIJ6KGGwbnX4bY4hXiCjbnSgO{1CSURic4ThbY5qdmdiYnHz[YQh[XO|YYm= NVnkenc6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G1N|k4PzlpPkOxOVM6Pzd7PD;hQi=>
HuH7 MlzYRZBweHSxc3nzJIF{e2G7 M1LxSFExOCC3TR?= NVviOoY2PDhiaILz MlW1TY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCKdVi3JINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKG6nY4LveIlkKGOnbHzzJIF1KDFyMDD1UUBqdmO3YnH0[YQh\m:{IES4JIhzeyCkeTDhco5mgGmwIG[gZY5lKDdvQVHEJJN1[WmwaX7nJIJie2WmIHHzd4F6 M4C0NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNUO5O|c6Lz5|MUWzPVc4QTxxYU6=
HuH7 NUXFNXV1SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NF3BO4wyODBidV2= NVznVFJzPDhiaILz NYrQVoV6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIeWg4KGOnbHzzJJRz[W6|ZnXjeIVlKHerdHigV4hUT0tzIHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiClZXzsJJZq[WKrbHn0fUBifCBzMECgeW0hcW6ldXLheIVlKG[xcjC0PEBpenNiYomgeJJ6eGGwIHLseYUh\HmnIHLhd4VlKGG|c3H5 M4DHb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNUO5O|c6Lz5|MUWzPVc4QTxxYU6=
HuH7 M37TXGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MVGxNFAhfU1? MVG0PEBpenN? MmrjRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKdVi3JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWJRmCgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGG2IEGwNEB2VSCrbnP1ZoF1\WRiZn;yJFQ5KGi{czDifUB1enmyYX6gZox2\SCmeXWgZoF{\WRiYYPzZZk> M3nCPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNUO5O|c6Lz5|MUWzPVc4QTxxYU6=
HuH7 MYDBdI9xfG:|aYOgZZN{[Xl? MkTqNVAxKHWP NXLHdoNKPDhiaILz M1jtOmlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iSIXIO{Bk\WyuczD0doFve2[nY4Tl[EB4cXSqIGPoV2dMOSCjc4Pld5Nm\CCjczDlZZJtgSCjcH;weI9{cXNiYYSgNVAxKHWPIHnuZ5Vj[XSnZDDmc5IhPDhiaILzJIJ6KGGwbnX4bY4hXiCjbnSgO{1CSURic4ThbY5qdmdiYnHz[YQh[XO|YYm= MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTV|OUe3PUc,OzF3M{m3O|k9N2F-
HuH7 NFn4OIJCeG:ydH;zbZMh[XO|YYm= MlK4NVAxKHWP NYTqb4duPDhiaILz NYjyNFRZUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDIeWg4KGOnbHzzJI93\XKneIDy[ZN{cW6pIFXHSnAh[XRiMUCwJJVOKGmwY4XiZZRm\CCob4KgOFghcHK|IHL5JIFvdmW6aX6gWkBidmRiNz3BRWQhe3SjaX7pcoch[mG|ZXSgZZN{[Xl? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTV|OUe3PUc,OzF3M{m3O|k9N2F-
Assay
Methods Test Index PMID
Western blot Nanog / Oct-4 / Sox-2 ; p-EGFR / EGFR / p-HER2 / HER2 / p-ERK / ERK / PTEN 21304978 20729295
Growth inhibition assay Cell viability 23272133
In vivo Resveratrol improves health and survival of mice on a high-calorie diet. Resveratrol (22.4 mg/kg/day) shifts the physiology of middle-aged mice on a high-calorie diet towards that of mice on a standard diet and significantly increases their survival. Resveratrol produces changes associated with longer lifespan, including increased insulin sensitivity, reduced insulin-like growth factor-1 (IGF-I) levels, increased AMP-activated protein kinase (AMPK) and peroxisome proliferator-activated receptor-γcoactivator 1α(PGC-1α) activity, increased mitochondrial number, and improved motor function. Resveratrol opposed the effects of the high-calorie diet in 144 out of 153 significantly altered pathways. [3] Resveratrol has been shown to inhibit the initiation and growth of tumors in a wide variety of rodent cancer models. Dose of Resveratrol as low as 200 μg/kg daily already shows efficacy in a rat model of colon carcinogenesis. At higher dose of 40 mg/kg, Resveratrol increases the survival of mice with subcutaneous neuroblastomas from 0% to 70%. Resveratrol inhibits vascularization in the corneal micropocket assay in mice at a dose of only 48 μg/kg when administered daily. [3] Resveratrol shows beneficial effects on heart disease. Resveratrol blocks the increase in platelet aggregation induced by a hypercholesterolaemic diet. Resveratrol increase expression of both endothelial and inducible nitric oxide synthase. In stroke-prone, spontaneously hypertensive rats, resveratrol significantly reduces markers of oxidative stress such as glycated albumin in serum, and 8-hydroxyguanos-ine in urine. Providing Resveratrol in drinking water for 15 days (1 mg/kg) is sufficient to improve the recovery in function and coronary flow of isolated hearts. [3] Resveratrol displays anti-inflammatory activity in vivo. Resveratrol significantly reduces both acute and chronic chemically induced oedema, lipopolysaccharide-induced airway inflammation and osteoarthritis, and helps to prevent allograft rejection. Intravenously administered Resveratrol decreases inflammation induced by ischaemia/reperfusion, oxidants generated by hypoxanthine/xanthine oxidase (HX/XO) or platelet-activating factor, but not leukotriene B4 in rats. [3] Resveratrol shows beneficial effects on stroke and brain damage. Resveratrol administered intravenously significantly decreased ischaemic volume and brain water content at the extremely low doses of 100 ng/kg and 1 μg/kg after middle cerebral artery occlusion in rats. [5]

Protocol (from reference)

Cell Research:

[6]

  • Cell lines: Human breast cancer cell MCF-7
  • Concentrations: 30-300 μM
  • Incubation Time: 2 days
  • Method:

    Cells are plated in flat-bottomed, 96-well microtiter plates (4000 cells/6.4-mm-diameter well). After 12–24 hr, cells are treated with DMSO (0.1–0.3%) or increasing doses of Resveratrol. After 48 h of treatment, cells are treated with 10μL of MTT reagent for 4 hr at 37 ℃ and then treated with 100 μL of solubilization solution at 37 ℃ overnight. The quantity of formazan product is measured using a spectrophotometric microtiter plate reader at 570 nm wavelength.

Animal Research:

[7]

  • Animal Models: Human ovarian xenografts PA-1
  • Dosages: 100 mg/kg
  • Administration: i.p. daily for consecutive 4 weeks

Solubility (25°C)

In vitro

DMSO 45 mg/mL warmed
(197.16 mM)
Water Insoluble
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
5% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.

15mg/mL

Chemical Information

Molecular Weight 228.24
Formula

C14H12O3

CAS No. 501-36-0
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC(=CC=C1C=CC2=CC(=CC(=C2)O)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03743636 Recruiting Drug: Nicotinamide riboside|Drug: Resveratrol|Other: Placebo Peripheral Artery Disease Northwestern University|American Heart Association October 1 2018 Phase 3

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Resveratrol (SRT501) | Resveratrol (SRT501) ic50 | Resveratrol (SRT501) price | Resveratrol (SRT501) cost | Resveratrol (SRT501) solubility dmso | Resveratrol (SRT501) purchase | Resveratrol (SRT501) manufacturer | Resveratrol (SRT501) research buy | Resveratrol (SRT501) order | Resveratrol (SRT501) mouse | Resveratrol (SRT501) chemical structure | Resveratrol (SRT501) mw | Resveratrol (SRT501) molecular weight | Resveratrol (SRT501) datasheet | Resveratrol (SRT501) supplier | Resveratrol (SRT501) in vitro | Resveratrol (SRT501) cell line | Resveratrol (SRT501) concentration | Resveratrol (SRT501) nmr