CA-5f

Catalog No.S5920

CA-5f Chemical Structure

Molecular Weight(MW): 388.46

CA-5f is a potent late-stage macroautophagy/autophagy inhibitor via inhibiting autophagosome-lysosome fusion.

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Biological Activity

Description CA-5f is a potent late-stage macroautophagy/autophagy inhibitor via inhibiting autophagosome-lysosome fusion.
Targets
Autophagy [1]
()
In vitro

CA-5f neither impairs the hydrolytic function nor the quantity of lysosomes. Treatment of human umbilical vein endothelial cells (HUVECs) with CA-5f for 1 h suppresses the levels of cytoskeletal proteins and membrane traffic proteins. CA-5f exhibits strong cytotoxicity against A549 non-small cell lung cancer (NSCLC) cells, but low cytotoxicity to normal human umbilical vein endothelial cells (HUVECs), by increasing mitochondrial-derived reactive oxygen species (ROS) production. CA-5f inhibits autophagic flux by blocking autophagosome-lysosome fusion instead of affecting the pH and hydrolytic function of lysosomes[1].

In vivo CA-5f effectively suppresses the growth of A549 lung cancer xenograft as a single agent with an excellent tolerance in vivo. It inhibits autophagic flux, induced apoptosis, and does not affect the level of CTSB (cathepsin B) and CTSD (cathepsin D) in vivo[1].

Protocol

Cell Research:

[1]

+ Expand
  • Cell lines: A549 cells or HUVECs
  • Concentrations: 20 μM
  • Incubation Time: 96 h
  • Method:

    A549 cells or HUVECs are seeded into E-Plate 16-well plates overnight and then treated with DMSO or CA-5f and continuously monitored for 96 h. The changes of cell index can be used to monitor cell viability.


    (Only for Reference)
Animal Research:

[1]

+ Expand
  • Animal Models: Four-week-old BALB/c nude mice (subcutaneous injection of A549 cells at the right scapula)
  • Formulation: DMSO
  • Dosages: 40 mg/kg
  • Administration: injected via caudal vein
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 78 mg/mL (200.79 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 388.46
Formula

C24H24N2O3

CAS No. 1370032-19-1
Storage powder
in solvent
Synonyms N/A

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Autophagy Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID