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SMER28 Autophagy activator

Cat.No.S8240

SMER28 is a small-molecule enhancer (SMER) of autophagy, inducing autophagy independently of rapamycin in mammalian cells.
SMER28 Autophagy activator Chemical Structure

Chemical Structure

Molecular Weight: 264.12

Jump to Mechanism of Action Cell Culture & Working Concentration Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Cell Culture, Treatment & Working Concentration

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
PC12 Function assay 43 uM Enhancement of autophagy in rat PC12 cells assessed as decrease in alpha-synuclein A53T mutant level at 43 uM by immunoblotting 17486044
COS7 Function assay 47 uM 48 hrs Inhibition of EGFP-tagged huntingtin aggregation expressed in african green monkey COS7 cells at 47 uM after 48 hrs by Western blot analysis 17486044
COS7 Function assay 47 uM 48 hrs Inhibition of EGFP-tagged huntingtin expressed in african green monkey COS7 cells assessed as protection against protein-induced cell death at 47 uM after 48 hrs by Western blot analysis 17486044
HeLa Function assay 47 uM 24 hrs Induction of autophagy in human HeLa cells harboring MAP1LC3 gene at 47 uM after 24 hrs by DAPI staining-based fluorescence microscopy 17486044
HeLa Function assay 47 uM 24 hrs Increase in MAP1LC3 level in bafilomycin A1-treated human HeLa cells at 47 uM treated fro 24 hrs before bafilomycin A1 challenge by immunoblotting analysis 17486044
PC12 Function assay 47 uM 8 hrs Enhancement of rapamycin-induced clearance of alpha-synuclein A53T mutant protein in rat PC12 cells at 47 uM after 8 hrs by immunoblotting 17486044
COS7 Function assay 47 uM 24 hrs Inhibition of EGFP-tagged huntingtin aggregation expressed in african green monkey COS7 cells at 47 uM after 24 hrs by Western blot analysis in presence of 0.2 uM rapamycin 17486044
PC12 Function assay 5 mg/ml Enhancement of autophagy in rat PC12 cells assessed as decrease in alpha-synuclein A53T mutant level at 5 mg/ml by immunoblotting 17486044
PC12 Function assay 5 mg/ml Enhancement of autophagy in rat PC12 cells assessed as decrease in alpha-synuclein A53T mutant level at 5 mg/ml by densitometry 17486044
PC12 Function assay 47 uM Enhancement of autophagy in rat PC12 cells assessed as decrease in alpha-synuclein A53T mutant level at 47 uM by immunoblotting 17486044
Click to View More Cell Line Experimental Data

Chemical Information, Storage & Stability

Molecular Weight 264.12 Formula

C11H10BrN3

 Storage (From the date of receipt)
CAS No. 307538-42-7 Download SDF Storage of Stock Solutions

Synonyms N/A Smiles C=CCNC1=NC=NC2=C1C=C(C=C2)Br

Solubility

In vitro
Batch:

DMSO : 53 mg/mL (200.66 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 53 mg/mL

Water : Insoluble

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo
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In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Mechanism of Action

Targets/IC50/Ki
Autophagy [1]
In vitro
SMER28 is a positive regulator of autophagy in a mechanism independent from the mTOR pathway. It increases autophagosome synthesis and enhances the clearance of model autophagy substrates such as A53T a-synuclein and mutant huntingtin fragments. A marked decrease in Aβ/APP-CTF levels and enhancement for degradation of Aβ/APP-CTF via autophagy is also induced by this compound. APP-CTF and LC3-II are cocompartmentalized on this chemical treatment[1].
References

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