Resveratrol

Catalog No.S1396 Batch:S139612

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Technical Data

Formula

C14H12O3
Molecular Weight 228.24 CAS No. 501-36-0
Solubility (25°C)* In vitro DMSO 300 mg/mL (1314.4 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 15.000mg/ml (65.72mM) Taking the 1 mL working solution as an example, add 50 μL of 300 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Resveratrol has a wide spectrum of targets including cyclooxygenases(i.e. COX, IC50=1.1 μM), lipooxygenases（LOX, IC50=2.7 μM）, kinases, sirtuins and other proteins. It has anti-cancer, anti-inflammatory, blood-sugar-lowering and other beneficial cardiovascular effects. This compound induces mitophagy/autophagy and autophagy-dependent apoptosis.
Targets
SIRT1 [2]
(Cell-free assay)		 SIRT2 [2]
(Cell-free assay)		 Quinone reductase 2 [9]
(Cell-free assay)		 IKK β [1]
(Cell-free assay)		 COX1 [9]
(Cell-free assay)		 View More
88 nM 1 μM 1.1 μM
In vitro Resveratrol inhibits the activity of Cyclooxygenase and lipooxygenase, PKCs and p56lck, ERK1, JNK1, p38, IKK β, Src, STAT3, Ribonucleotide Reductase, DNA polymerases α and δ, PKD, PKC α, Quinone reductase 2, and Aromatase with IC50 of from 0.035-60 μM. This compound is also an activator of Adenylyl cyclase and AMPK with EC50 of 0.8 μM and 50 μM, respectively. The roles of this compound as inhibitor or activator enable its effects on decreasing cell inflammatory associated behaviors, growth inhibition and induction of apoptosis in cancer cells, reversal of endothelin-1 stimulated cell responses, inhibition of phorbol ester-induced expression of COX-2, inhibition of DNA synthesis in cell, resistance to menadione-induced cell death, and improvement of cell mitochondrial function and glucose/lipid metabolism. [1] It is also an activator of sirtuins. This chemical lowers the Michaelis constant of SIRT1 for both the acetylated substrate and NAD+, and increases cell survival by stimulating SIRT1-dependent deacetylation of p53. In yeast, it mimics calorie restriction by stimulating Sir2, increasing DNA stability and extending lifespan. [2] This compound is effective at protecting isolated rat hearts against ischemia/reperfusion injury via its antioxidant activity, with improved recovery of developed pressure and aortic flow, reduction of malondialdehyde concentrations and reduction of infarct size. [3]
In vivo Resveratrol improves health and survival of mice on a high-calorie diet. This compound (22.4 mg/kg/day) shifts the physiology of middle-aged mice on a high-calorie diet towards that of mice on a standard diet and significantly increases their survival. It produces changes associated with longer lifespan, including increased insulin sensitivity, reduced insulin-like growth factor-1 (IGF-I) levels, increased AMP-activated protein kinase (AMPK) and peroxisome proliferator-activated receptor-γcoactivator 1α(PGC-1α) activity, increased mitochondrial number, and improved motor function. The substance opposed the effects of the high-calorie diet in 144 out of 153 significantly altered pathways. [3] It has been shown to inhibit the initiation and growth of tumors in a wide variety of rodent cancer models. Dose of this chemical as low as 200 μg/kg daily already shows efficacy in a rat model of colon carcinogenesis. At higher dose of 40 mg/kg, it increases the survival of mice with subcutaneous neuroblastomas from 0% to 70%. This compound inhibits vascularization in the corneal micropocket assay in mice at a dose of only 48 μg/kg when administered daily. [3] It shows beneficial effects on heart disease. The substance blocks the increase in platelet aggregation induced by a hypercholesterolaemic diet. It increase expression of both endothelial and inducible nitric oxide synthase. In stroke-prone, spontaneously hypertensive rats, resveratrol significantly reduces markers of oxidative stress such as glycated albumin in serum, and 8-hydroxyguanos-ine in urine. Providing this chemical in drinking water for 15 days (1 mg/kg) is sufficient to improve the recovery in function and coronary flow of isolated hearts. [3] It displays anti-inflammatory activity in vivo. This compound significantly reduces both acute and chronic chemically induced oedema, lipopolysaccharide-induced airway inflammation and osteoarthritis, and helps to prevent allograft rejection. Intravenously administered resveratrol decreases inflammation induced by ischaemia/reperfusion, oxidants generated by hypoxanthine/xanthine oxidase (HX/XO) or platelet-activating factor, but not leukotriene B4 in rats. [3] It shows beneficial effects on stroke and brain damage. The substance administered intravenously significantly decreased ischaemic volume and brain water content at the extremely low doses of 100 ng/kg and 1 μg/kg after middle cerebral artery occlusion in rats. [5]

Protocol (from reference)

Cell Assay:

[6]
  • Cell lines

    Human breast cancer cell MCF-7

  • Concentrations

    30-300 μM

  • Incubation Time

    2 days

  • Method

    Cells are plated in flat-bottomed, 96-well microtiter plates (4000 cells/6.4-mm-diameter well). After 12–24 hr, cells are treated with DMSO (0.1–0.3%) or increasing doses of Resveratrol. After 48 h of treatment, cells are treated with 10μL of MTT reagent for 4 hr at 37 ℃ and then treated with 100 μL of solubilization solution at 37 ℃ overnight. The quantity of formazan product is measured using a spectrophotometric microtiter plate reader at 570 nm wavelength.
Animal Study:

[7]
  • Animal Models

    Human ovarian xenografts PA-1

  • Dosages

    100 mg/kg

  • Administration

    i.p. daily for consecutive 4 weeks

References

  • https://pubmed.ncbi.nlm.nih.gov/18421779/
  • https://pubmed.ncbi.nlm.nih.gov/12939617/
  • https://pubmed.ncbi.nlm.nih.gov/16732220/
  • https://pubmed.ncbi.nlm.nih.gov/17086191/
  • https://pubmed.ncbi.nlm.nih.gov/12887737/
  • https://pubmed.ncbi.nlm.nih.gov/11895924/
  • https://pubmed.ncbi.nlm.nih.gov/19738051/
  • https://pubmed.ncbi.nlm.nih.gov/16611627/
  • https://pubmed.ncbi.nlm.nih.gov/20450491/

Customer Product Validation

<p>Cellular senescence and SIRT1 phosphorylation was monitored in PAECs (P2) incubated in DMEM containing HDL (50 mg/L), LDL (50 mg/L), or resveratrol (100 μM) for 24 hours.</p>

, , Dr. Johanna Weiss of University Hospital Heidelberg

PC-9 and A549 cells were treated with 100 nM siRNA against PUMA and 100 nM negative control (NC) siRNA for 12 h, and treated with the indicated concentrations of RV and ERL in combination for an additional 48 h. The knockdown effects on PUMA were confirmed by Western blot analysis.

Data from [ , , Cell Physiol Biochem, 2015, 35: 2255-2271 ]

Selleck's Resveratrol Has Been Cited by 79 Publications

Identifying Age-Modulating Compounds Using a Novel Computational Framework for Evaluating Transcriptional Age [ Aging Cell, 2025, e70075] PubMed: 40307992
MIT-001 ameliorates ferroptosis-induced mitochondrial dysfunction and enhances embryo quality in preimplantation embryos from aged female mice [ Biomed Pharmacother, 2025, 190:118393] PubMed: 40784125
The nonsteroidal anti-inflammatory drug sulindac reverses obesity-driven immunosuppression and triple-negative breast cancer progression [ Breast Cancer Res, 2025, 27(1):186] PubMed: 41137072
Resveratrol contributes to NK cell-mediated breast cancer cytotoxicity by upregulating ULBP2 through miR-17-5p downmodulation and activation of MINK1/JNK/c-Jun signaling [ Front Immunol, 2025, 16:1515605] PubMed: 39963142
Bisphenol A induces cellular senescence in SW1353 chondrocytes via miRNA-449a-mediated downregulation of SIRT1 [ Ecotoxicol Environ Saf, 2025, 303:118906] PubMed: 40848421
Lovastatin and Resveratrol Synergistically Improve Wound Healing and Inhibit Bacterial Growth [ Int J Mol Sci, 2025, 26(2)851] PubMed: 39859566
Vitamin A acts as a potent suppressor of selenoprotein P with potential relevance for multivitamin supplementation [ J Nutr Biochem, 2025, 148:110142] PubMed: 41106613
Cytoprotective role of resveratrol in cigarette smoke-induced pyroptosis through Nrf2 pathway activation [ Cell Stress Chaperones, 2025, 30(5):100107] PubMed: 40744391
Resveratrol aggravated H2O2-induced the HK-2 cell damage by inhibiting AKT phosphorylation [ PLoS One, 2025, 20(7):e0327135] PubMed: 40743123
Resveratrol mediated the proliferation and apoptosis of gastric cancer cells by modulating the PI3K/Akt/P53 signaling pathway [ Biochem Biophys Res Commun, 2024, 723:150186] PubMed: 38830298

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.