Molecular Weight(MW): 271.26
Spautin-1 is a potent and specific autophagy inhibitor, and inhibits the deubiquitinating activity of USP10 and USP13 with IC50 of ∼0.6-0.7 μM.
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Western blot analyses show significant inhibition of autophagy-related gene expression in Spautin-1 treated OCI-AML2 cells after Ara-C treatment.
Cancer Lett, 2018, 425:1-12. Spautin-1 purchased from Selleck.
Autophagosomal inhibition was performed in 32D EV, WT CALR, del52, and ins5 expressing cells. The 32D cell lines were treated with the inhibitor spautin-1 for the indicated time. Lysates were prepared, and SDS-PAGE and Western blotting were performed. Antibodies detecting mutated CALR (CALR mut), CALR, LC3I-II, and GAPDH were used for immunostaining. LC3I-II served as control for successful autophagosomal inhibition.
J Hematol Oncol, 2016, 9(1):45. Spautin-1 purchased from Selleck.
(c) PC-9G cells were treated with BIBW2992 (250 nM) or WZ4002 (15 nM) plus SAHA (2 mM) with or without spautin-1 (20 mM) for 24 hr. The cell lysates were harvested and the indicated proteins were analyzed by Western blotting.
Int J Cancer, 2015, 136(11):2717-29. . Spautin-1 purchased from Selleck.
Male C57BL/6 mice (n = 8 for each group) were instilled intratracheally with normal saline (NS), PM (100 μg/d), spautin-1 (0.5 mg/d), or PM plus spautin-1 respectively for 2 days, and after 24 h, representative images of lung tissue stained with H&E
Toxicol Lett, 2017, 280:206-212. Spautin-1 purchased from Selleck.
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Choose Selective Autophagy Inhibitors
|Description||Spautin-1 is a potent and specific autophagy inhibitor, and inhibits the deubiquitinating activity of USP10 and USP13 with IC50 of ∼0.6-0.7 μM.|
In Bcap-37 cells, Spautin-1 dramatically enhanced cell death in glucose-free media and induces apoptotic morphology. In Bax-Bak DKO cells, spautin-1 inhibits etoposide induced autophagic cell death. Spautin-1 promots the degradation of Vps34 complexes by regulating the deubiquitination activity of USP10 and USP13, and reduces the levels of PtdIns3P.  In PDGF-treated cells, spautin-1 stabilizes α-smooth muscle cell actin and calponin, prevents actin filament disorganization, diminishes production of extracellular matrix, and abrogates VSMC hyperproliferation and migration.  In CML cells, spautin-1 markedly inhibits IM-induced autophagy by downregulating Beclin-1, and enhances IM-induced apoptosis by inactivating PI3K/AKT and activating downstream GSK3β.  Spautin-1 also specifically reduces infectious dengue virus titers in BHK-21 cells. 
|In vitro||DMSO||54 mg/mL (199.07 mM)|
|Ethanol||7 mg/mL (25.8 mM)|
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Molecular Weight Calculator
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