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Catalog No.S1680 Synonyms: NSC 190940

12 publications

Disulfiram  Chemical Structure

CAS No. 97-77-8

Disulfiram (NSC 190940) is a specific inhibitor of aldehyde-dehydrogenase (ALDH) with IC50 of 0.15 μM and 1.45 μM for hALDH1 and hALDH2, respectively. Disulfiram is used for the treatment of chronic alcoholism by producing an acute sensitivity to alcohol. Disulfiram induces apoptosis.

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10mM (1mL in DMSO) USD 130 In stock
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Selleck's Disulfiram has been cited by 12 publications

1 Customer Review

  • The effect of drugs on sperm movement. Purified human sperm were incubated under capacitating conditions for 0, 15, 30, 60 or 120 min, and motility was measured in the presence of disulfirum. The standard deviation is shown as bars. Statistical differences by Student's t-test compared with control are annotated as “*” for p<0.05 or “**” for p<0.01.

    J Proteomics 2013 79, 114-22. Disulfiram purchased from Selleck.

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Biological Activity

Description Disulfiram (NSC 190940) is a specific inhibitor of aldehyde-dehydrogenase (ALDH) with IC50 of 0.15 μM and 1.45 μM for hALDH1 and hALDH2, respectively. Disulfiram is used for the treatment of chronic alcoholism by producing an acute sensitivity to alcohol. Disulfiram induces apoptosis.
ALDH1 [5]
(Cell-free assay)
ALDH2 [5]
(Cell-free assay)
0.15 μM 1.45 μM
In vitro

Disulfiram-copper complex potently inhibits the proteasomal activity in cultured breast cancer MDA-MB-231 and MCF10DCIS.com cells, but not normal, immortalized MCF-10A cells, before induction of apoptotic cancer cell death. [1] Disulfiram (DS), a clinically used anti-alcoholism drug, strongly inhibits constitutive and 5-FU-induced NF-kappaB activity in a dose-dependent manner. Disulfiram inhibits both NF-kappaB nuclear translocation and DNA binding activity but has no effect on 5-FU-induced IkappaBalpha degradation. Disulfiram significantly enhances the apoptotic effect of 5-FU on DLD-1 and RKO(WT) cell lines and synergistically potentiated the cytotoxicity of 5-FU to both cell lines. Disulfiram also effectively abolishes 5-FU chemoresistance in a 5-FU resistant cell line H630(5-FU) in vitro. [2] Oseltamivir decreases the number of viable cells, and the addition of CuCl(2) significantly enhances the DSF-induced cell death to less than 10% of control. [3] Disulfiram given to melanoma cells in combination with Cu2+ or Zn2+ decreases expression of cyclin A and reduces proliferation in vitro at lower concentrations than disulfiram alone. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MCF7 cells NW\TOVhxWHKxbHnm[ZJifGmxbjDhd5NigQ>? M1fnOVczKGh? NHPnSZdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOg[ZhxemW|c3nu[{BDS0F{IHHu[EBme3S{b3flckBz\WOncITvdkBi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUCuNUDPxE1? MWWyNFIzOjZ5MR?=
human T47D cells MVnQdo9tcW[ncnH0bY9vKGG|c3H5 MUe3NkBp M1LE[2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iVES3SEBk\WyuczDlfJBz\XO|aX7nJGJESTJiYX7kJIV{fHKxZ3XuJJJm[2WydH;yJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD6xO{DPxE1? MnO3NlAzOjJ4N{G=
human MDA-MB-231 cells MUPQdo9tcW[ncnH0bY9vKGG|c3H5 MXe3NkBp MlfQRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGW6cILld5NqdmdiQlPBNkBidmRiRWLhcJBp[SCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvOzJizszN MmLINlAzOjJ4N{G=
CHO cells MoDrSpVv[3Srb36gZZN{[Xl? MoDTRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDUVnBCOSClaHHucoVtKGW6cILld5Nm\CCrbjDDTG8h[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iaX70doFk\WyudXzhdkBk[WylaYXtJIxmfmWuczygSWM2OD1|IN88US=> M1\4TlIxOzV4M{C1
human MCF10A cells NEfNSHNRem:uaX\ldoF1cW:wIHHzd4F6 MYW3NkBp Mn3MRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[xNGEh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zMDFOwG0> MXOyNFIzOjZ5MR?=

... Click to View More Cell Line Experimental Data

In vivo

Disulfiram significantly inhibits the tumor growth (by 74%), associated with in vivo proteasome inhibition (as measured by decreased levels of tumor tissue proteasome activity and accumulation of ubiquitinated proteins and natural proteasome substrates p27 and Bax) and apoptosis induction (as shown by caspase activation and apoptotic nuclei formation) in mice bearing MDA-MB-231 tumor xenografts. [1] Disulfiram blocks the P-glycoprotein extrusion pump, inhibits the transcription factor nuclear factor-kappaB, sensitizes tumors to chemotherapy, reduces angiogenesis, and inhibits tumor growth in mice. Disulfiram inhibits growth and angiogenesis in melanomas transplanted in severe combined immunodeficient mice, and these effects are potentiated by Zn2+ supplementation. [4]


Solubility (25°C)

In vitro DMSO 59 mg/mL (198.96 mM)
Water Insoluble
Ethanol '59 mg/mL
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 296.54


CAS No. 97-77-8
Storage powder
in solvent
Synonyms NSC 190940

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04485130 Not yet recruiting Drug: Disulfiram|Drug: Placebo Covid19 University of California San Francisco December 1 2020 Phase 2
NCT03151772 Terminated Drug: Disulfiram|Drug: Metformin Glioblastoma Sahlgrenska University Hospital Sweden January 29 2018 Early Phase 1
NCT02309801 Completed Dietary Supplement: Daidzin|Dietary Supplement: Alcohol Healthy Parc de Salut Mar|Ministerio de Sanidad Servicios Sociales e Igualdad July 2012 Phase 1
NCT01286259 Completed Drug: Disulfiram HIV-1 Infection University of California San Francisco|Johns Hopkins University January 2011 Not Applicable
NCT02101008 Completed Drug: disulfiram and chelated zinc Melanoma University of Utah March 2010 Phase 2

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID