Disulfiram

Catalog No.S1680 Synonyms: NSC 190940

Disulfiram  Chemical Structure

Molecular Weight(MW): 296.54

Disulfiram is a specific inhibitor of aldehyde-dehydrogenase (ALDH1), used for the treatment of chronic alcoholism by producing an acute sensitivity to alcohol.

Size Price Stock Quantity  
In DMSO USD 130 In stock
USD 97 In stock
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1 Customer Review

  • The effect of drugs on sperm movement. Purified human sperm were incubated under capacitating conditions for 0, 15, 30, 60 or 120 min, and motility was measured in the presence of disulfirum. The standard deviation is shown as bars. Statistical differences by Student's t-test compared with control are annotated as “*” for p<0.05 or “**” for p<0.01.

    J Proteomics 2013 79, 114-22. Disulfiram purchased from Selleck.

Purity & Quality Control

Choose Selective Dehydrogenase Inhibitors

Biological Activity

Description Disulfiram is a specific inhibitor of aldehyde-dehydrogenase (ALDH1), used for the treatment of chronic alcoholism by producing an acute sensitivity to alcohol.
Targets
ALDH1 [1]
In vitro

Disulfiram-copper complex potently inhibits the proteasomal activity in cultured breast cancer MDA-MB-231 and MCF10DCIS.com cells, but not normal, immortalized MCF-10A cells, before induction of apoptotic cancer cell death. [1] Disulfiram (DS), a clinically used anti-alcoholism drug, strongly inhibits constitutive and 5-FU-induced NF-kappaB activity in a dose-dependent manner. Disulfiram inhibits both NF-kappaB nuclear translocation and DNA binding activity but has no effect on 5-FU-induced IkappaBalpha degradation. Disulfiram significantly enhances the apoptotic effect of 5-FU on DLD-1 and RKO(WT) cell lines and synergistically potentiated the cytotoxicity of 5-FU to both cell lines. Disulfiram also effectively abolishes 5-FU chemoresistance in a 5-FU resistant cell line H630(5-FU) in vitro. [2] Oseltamivir decreases the number of viable cells, and the addition of CuCl(2) significantly enhances the DSF-induced cell death to less than 10% of control. [3] Disulfiram given to melanoma cells in combination with Cu2+ or Zn2+ decreases expression of cyclin A and reduces proliferation in vitro at lower concentrations than disulfiram alone. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MCF7 cells MYrQdo9tcW[ncnH0bY9vKGG|c3H5 MY[3NkBp MVzBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiZYjwdoV{e2mwZzDCR2EzKGGwZDDld5Rzd2enbjDy[YNmeHSxcjDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTBwMTFOwG0> NHraWVQzODJ{Mk[3NS=>
human T47D cells MmDTVJJwdGmoZYLheIlwdiCjc4PhfS=> MV[3NkBp NYDwcHRFSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDUOFdFKGOnbHzzJIV5eHKnc4PpcochSkODMjDhcoQh\XO2cn;n[Y4hemWlZYD0c5Ih[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlE4KM7:TR?= NV[3W|BjOjB{MkK2O|E>
human MDA-MB-231 cells M4XqZXBzd2yrZnXyZZRqd25iYYPzZZk> NVHqS|JLPzJiaB?= Mlv6RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGW6cILld5NqdmdiQlPBNkBidmRiRWLhcJBp[SCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvOzJizszN NHn0PJczODJ{Mk[3NS=>
CHO cells NX\qXlFrTnWwY4Tpc44h[XO|YYm= MmjPRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDUVnBCOSClaHHucoVtKGW6cILld5Nm\CCrbjDDTG8h[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iaX70doFk\WyudXzhdkBk[WylaYXtJIxmfmWuczygSWM2OD1|IN88US=> MWCyNFM2PjNyNR?=
human MCF10A cells MUDQdo9tcW[ncnH0bY9vKGG|c3H5 NFfDeXU4OiCq M2fRUWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGNVBCKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MUCg{txO MmWzNlAzOjJ4N{G=

... Click to View More Cell Line Experimental Data

In vivo Disulfiram significantly inhibits the tumor growth (by 74%), associated with in vivo proteasome inhibition (as measured by decreased levels of tumor tissue proteasome activity and accumulation of ubiquitinated proteins and natural proteasome substrates p27 and Bax) and apoptosis induction (as shown by caspase activation and apoptotic nuclei formation) in mice bearing MDA-MB-231 tumor xenografts. [1] Disulfiram blocks the P-glycoprotein extrusion pump, inhibits the transcription factor nuclear factor-kappaB, sensitizes tumors to chemotherapy, reduces angiogenesis, and inhibits tumor growth in mice. Disulfiram inhibits growth and angiogenesis in melanomas transplanted in severe combined immunodeficient mice, and these effects are potentiated by Zn2+ supplementation. [4]

Protocol

Solubility (25°C)

In vitro DMSO 59 mg/mL (198.96 mM)
Ethanol 59 mg/mL (198.96 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 296.54
Formula

C10H20N2S4

CAS No. 97-77-8
Storage powder
in solvent
Synonyms NSC 190940

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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Molecular Weight Calculator

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03034135 Active not recruiting Recurrent Glioblastoma Cantex Pharmaceuticals March 9 2017 Phase 2
NCT03198559 Suspended HIV Infections The Peter Doherty Institute for Infection and Immunity|Merck Sharp & Dohme Corp.|The Alfred|University of Melbourne August 8 2017 Phase 1|Phase 2
NCT03151772 Recruiting Glioblastoma Sahlgrenska University Hospital Sweden January 29 2018 Early Phase 1
NCT03323346 Recruiting Breast Neoplasm Female|Metastatic Breast Cancer Marian Hajduch M.D. Ph.D.|University Hospital Olomouc|The Institute of Molecular and Translational Medicine Czech Republic September 29 2017 Phase 2
NCT03363659 Recruiting Glioblastoma|Glioblastoma Multiforme Aurora Health Care March 28 2018 Phase 2
NCT02671890 Suspended Metastatic Pancreatic Adenocarcinoma|Recurrent Pancreatic Carcinoma|Solid Neoplasm|Stage IV Pancreatic Cancer Mayo Clinic|National Cancer Institute (NCI) February 25 2016 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Dehydrogenase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID