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Gossypol Acetate

Name: Gossypol Acetate
Brand: Selleck Chemicals
SKU: S2303
Rating: 5 (15 reviews)

Catalog No.S2303 Synonyms: Gossypol Acetic acid, Pogosin, AT101

For research use only.

Gossypol Acetate (Gossypol Acetic acid, Pogosin, AT101) is a polyphenolic aldehyde that permeates cells and acts as an inhibitor for several dehydrogenase enzymes such as lactate dehydrogenase, NAD-linked enzymes.

CAS No. 12542-36-8

Selleck's Gossypol Acetate has been cited by 15 publications

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Dehydrogenase Signaling Pathway Map

Biological Activity

Description

Targets

Dehydrogenase ^[1]

Bcl2 ^[5]

In vitro

Gossypol, a known antispermatogenic agent from the cotton plant genus Gossypium, was found to inhibit yellow perch sperm motility in vitro and lactate dehydrogenase activity in spermatozoa when used in a dose-dependent manner^[1]. Gossypol has been approved to have antiproliferative and apoptosis-inducing effects on some kinds of cancer cell lines in vitro^[2].

In vivo

Gossypol acetate is able to inhibit tumor growth in Wus1-bearing mice, but the survival of mice is not prolonged, and tumor grows rapidly after short inhibition. Gossypol has now been found to have inhibitory effects on proliferation or to induce apoptosis in ovarian cancer, endometrial cancer, adrenal cortical tumor, thyroid cancer, lung cancer, colon carcinoma, leukemia, pancreatic cancer, melanoma and lymphoma. In addition, gossypol can increase the sensitivity of drug-resistant tumor cells to chemotherapy and radiotherapy. Some clinical trials showed gossypol is well-tolerated, and partial responses are observed in some patients^[4].

Protocol (from reference)

Cell Research:

^[4]

Cell lines: Human MM cell lines U266 and Wus1
Concentrations: 1, 5, 10, 25 and 50 µmol/l
Incubation Time: 0, 24, 48 and 72 h
Method:
MM(Multiple myeloma) cells are plated in 24-cell culture clusters at a density of 1x105 viable cells/l per well. Triplicate wells are treated with 1, 5, 10, 25 and 50 µmol/l gossypol acetate, and the negative control group is supplemented with 0.1% DMSO. Then, cell numbers at different treatment time points (0, 24, 48 and 72 h) are determined by using a hemocytometer and the trypan blue dye-exclusion method. The trypan blue dye-exclusion method is used to evaluate the cell viability. The cells are examined in a counting chamber under a light microscope. Only viable cells are recorded.

References

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Solubility (25°C)

In vitro

Chemical Information

Molecular Weight	578.61
Formula	C₃₂H₃₄O₁₀
CAS No.	12542-36-8
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	CC1=CC2=C(C(=C(C(=C2C(C)C)O)O)C=O)C(=C1C3=C(C4=C(C=C3C)C(=C(C(=C4C=O)O)O)C(C)C)O)O.CC(=O)O

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
S2812 and S2303 share same structures. However, S2812 is described as ‘R’ enantiomer. What’s the difference between S2812 and S2303?

Answer:
S2303 is the mixture of S2812 and its enantiomer.

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