ML390

Catalog No.S8382

For research use only.

ML390 is a human DHODH inhibitor with an IC50 of 0.56 μM and induces differentiation in acute myeloid leukemia.

ML390 Chemical Structure

CAS No. 2029049-79-2

Selleck's ML390 has been cited by 1 Publication

Purity & Quality Control

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Biological Activity

Description ML390 is a human DHODH inhibitor with an IC50 of 0.56 μM and induces differentiation in acute myeloid leukemia.
Targets
DHODH [1]
(Cell-free assay)
0.56 μM
In vitro

ML390 was active with an ED50 (effective concentration triggering 50% of its maximal differentiation activity) of 2 μM in murine and human AML cell lines, offering insight into the mechanism of overcoming differentiation arrest. DHODH inhibitors demonstrated effective at prolonging survival in animal models of leukemia[1].

Protocol (from reference)

Cell Research:[2]
  • Cell lines: Lys-GFP-ER-HoxA9 cells
  • Concentrations: 10 μM
  • Incubation Time: 48 h
  • Method: Lys-GFP-ER-HoxA9 cells were treated in culture with 10 μM ML390 for 48 hr. The cells were washed three times in normal saline, and metabolites were extracted into 80% ice-cold methanol. Metabolites were analyzed. Negative ionization mode analyses of polar metabolites were acquired using an LC-MS system comprising an Acquity UPLC System (Waters) and a 5500 QTrap triple quadrupole mass spectrometer (AB SCIEX). Samples for negative ion mode analyses of polar metabolites were achieved using the HILIC (hydrophilic interaction chromatography) method under basic conditions as described previously, and MS data were acquired over m/z 70-750. MS data were processed using MultiQuant.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 406.4
Formula

C21H21F3N2O3

CAS No. 2029049-79-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1CC(C2=CC=CC=C2C1)NC(=O)CCNC(=O)C3=CC=C(C=C3)OC(F)(F)F

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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