G6PDi-1

Catalog No.S9617

For research use only.

G6PDi-1 is an effective inhibitor of glucose-6-phosphate dehydrogenase (G6PD). G6PDi-1 depletes NADPH and decreases inflammatory cytokine production.

G6PDi-1 Chemical Structure

CAS No. 2457232-14-1

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Biological Activity

Description G6PDi-1 is an effective inhibitor of glucose-6-phosphate dehydrogenase (G6PD). G6PDi-1 depletes NADPH and decreases inflammatory cytokine production.
Targets
G6PD [1]
()
NADPH [1]
()
In vitro

G6PDi-1 effectively inhibits G6PD. Across a range of cultured cells, G6PDi-1 depletes NADPH most strongly in lymphocytes. In T cells but not macrophages, G6PDi-1 markedly decreases inflammatory cytokine production. In neutrophils, it suppresses respiratory burst.[1]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: HepG2 cells, HCT116 cells, T cells
  • Concentrations: 25 μM, 50 μM, 75 μM, 100 μM
  • Incubation Time: 2 h, 4 h, 6 h
  • Method:

    To analyze intracellular cytokines, active T cells are re-stimulated with PMA (20 ng/ml) and ionomycin (1 ug/ml) in the presence of GolgiStop and increasing concentrations of G6PDi-1. After a 6h incubation period, cells are collected, washed with PBS and stained with the viability dye Live/Dead Aqua.
    For measuring intracellular ROS levels, cells are incubated for 2 h in the presence of increasing concentrations of G6PDi-1 and then stained for ROS and viability using CellROX Green Flow Cytometry Assay Kit following manufacturer instructions.

Solubility (25°C)

In vitro

DMSO 57 mg/mL
(200.46 mM)
Water Insoluble
Ethanol Insoluble

Chemical Information

Molecular Weight 284.34
Formula

C14H12N4OS

CAS No. 2457232-14-1
Storage 3 years -20°C powder
2 years -80°C in solvent

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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