Home Metabolism Dehydrogenase agonist Alda 1 For research use only.

Alda 1

Alda 1 is an ALDH2 agonist that activates catalysis for both wild-type ALDH2 (ALDH2*1) and ALDH2*2.

Alda 1 Chemical Structure

Alda 1 Chemical Structure

CAS: 349438-38-6

Selleck's Alda 1 has been cited by 1 publication

Purity & Quality Control

Batch: S580001 Purity: 99.99%
99.99

Alda 1 Related Products

Signaling Pathway

Dehydrogenase Signaling Pathways

Dehydrogenase Signaling Pathway

Choose Selective Dehydrogenase Inhibitors

Biological Activity

Description Alda 1 is an ALDH2 agonist that activates catalysis for both wild-type ALDH2 (ALDH2*1) and ALDH2*2.
Targets
ALDH2 [1]
In vitro
In vitro Alda-1 alleviated liver injury after hepatic I/R. Pretreatment with Alda-1 significantly alleviated I/R-induced elevations of alanine aminotransferase and aspartate aminotransferase, and significantly blunted the pathological injury of the liver. Moreover, Alda-1 significantly inhibited ROS and proinflammatory cytokines production, 4-HNE and MDA accumulation, and apoptosis. Increased ALDH2 activity was found after Alda-1 administration. No significant changes in ALDH2 expression were observed after I/R[2].
Cell Research Cell lines normal rat hepatocyte cell (BRL-3A)
Concentrations 20 μM
Incubation Time 1 hour
Method

The H/R (Hypoxia/reoxygenation) group cells are incubated under a hypoxic atmosphere (95% N2+5% CO2) for 6 h followed by 120 min reoxygenation (95% air + 5% CO2). The control incubation is normoxic conditions (95% air + 5% CO2) for the entire experiment. In the H/R+Alda-1 group, cells are incubated with Alda-1 (20 μM) for 60 min at 37℃ (95% air/5% CO2) before exposure to hypoxia. Furthermore, BRL-3A cells are treated with 5 μM 4-HNE for 60min before exposure to hypoxia to estimate whether 4-HNE could affect H/R.

Chemical Information & Solubility

Molecular Weight 324.16 Formula

C15H11Cl2NO3
CAS No. 349438-38-6 SDF --
Smiles C1OC2=C(O1)C=C(C=C2)CNC(=O)C3=C(C=CC=C3Cl)Cl
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Alda 1 | Alda 1 supplier | purchase Alda 1 | Alda 1 cost | Alda 1 manufacturer | order Alda 1 | Alda 1 distributor