Brequinar (DUP785)

Catalog No.S6626 Synonyms: NSC 368390

For research use only.

Brequinar (DUP785, NSC 368390) is an inhibitor of dihydroorotate dehydrogenase (DHODH) with an IC50 of ∼20 nM in vitro.

Brequinar (DUP785) Chemical Structure

CAS No. 96187-53-0

Selleck's Brequinar (DUP785) has been cited by 3 Publications

Purity & Quality Control

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Biological Activity

Description Brequinar (DUP785, NSC 368390) is an inhibitor of dihydroorotate dehydrogenase (DHODH) with an IC50 of ∼20 nM in vitro.
Targets
DHODH [1]
20 nM
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
insect cells M2Hi[GZ2dmO2aX;uJIF{e2G7 M3\0WGlvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiRFjPSGgh\XiycnXzd4VlKGmwIHLhZ5Vtd3[rcoXzJIlv\mWldHXkJIlve2WldDDj[YxteyC3c3nu[{BlcWi7ZILvc5JwfGG2ZTDhd{B{fWK|dILheIUhcW5icILld4Vv[2Vib3[gdZVqdm:wZTDifUBlcWOqbH;yc5Bp\W6xbD3pcoRweGinbn;sJIR6\SCkYYPl[EBie3OjeTygTWM2OD1yLkCx{txO MnTxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd7OUS3OFgoRjJ5OUm0O|Q5RC:jPh?=
A549 MUXBcpRqfmm{YXygZZN{[Xl? Mln5RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgXYVtdG:5IH\leoVzKH[rcoXzJIlv\mWldHXkJIlvKGi3bXHuJGE2PDliY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJJZqenW|IILldIxq[2G2aX;uMEBGSzVyPUCuNFc5|ryP NX2zV2xbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G1OVc3OTJpPkOxOVU4PjF{PD;hQi=>
A549 NIrQWGNCdnSrdnnyZYwh[XO|YYm= NYHJ[pBXSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhX2W|dDDObYxmKH[rcoXzJIlv\mWldHXkJIlvKGi3bXHuJGE2PDliY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJJZqenW|IILldIxq[2G2aX;uMEBGSzVyPUCuNFc5|ryP NYm0cHp6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G1OVc3OTJpPkOxOVU4PjF{PD;hQi=>
A549 M1KzcGFvfGm4aYLhcEBie3OjeR?= M2\wNmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGRmdme3ZTD2bZJ2eyCrbn\lZ5Rm\CCrbjDoeY1idiCDNUS5JINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiC4aYL1d{Bz\XCuaXPheIlwdixiRVO1NF0xNjB5ON88US=> MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTV3N{[xNkc,OzF3NUe2NVI9N2F-
A549 NFLhTopCdnSrdnnyZYwh[XO|YYm= NXi1NYhvSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhX2W|dHXyckBmeXWrbnWg[Y5k\XCqYXzvcZlmdGm2aYOgeolzfXNiaX7m[YN1\WRiaX6gbJVu[W5iQUW0PUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5idnnyeZMhemWybHnjZZRqd25uIFXDOVA:OC5yN{lOwG0> NUHqbWgxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G1OVc3OTJpPkOxOVU4PjF{PD;hQi=>
A549 NXz2fIR5SW62aY\pdoFtKGG|c3H5 NUnYOYZDSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhXmW|aXP1cIFzKHO2b33heIl1cXNidnnyeZMhcW6oZXP0[YQhcW5iaIXtZY4hSTV2OTDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hfmm{dYOgdoVxdGmlYYTpc44tKEWFNUC9NE4xPzkQvF2= M1rDOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNUW3OlEzLz5|MUW1O|YyOjxxYU6=
Jurkat Mni2RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NX22TWZKPzJiaILz NXTwWVdUUW6qaXLpeIlwdiCxZjDj[YxtKHC{b3zp[oVz[XSrb36gc4YhcHWvYX6gTpVzc2G2IHPlcIx{KGmwY4XiZZRm\CCob4KgO|IhcHK|IHL5JGNmdGy2aYTldk1IdG9iYYPzZZktKEmFNUC9NE4z|ryP M2\TZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEe5NFQ{Lz5{NkC3PVA1OzxxYU6=
MDCK M37t[mFvfGm4aYLhcEBie3OjeR?= Mnm2OFghcHK| Mn:xRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgWnNXKGmwZnXjeIVlKGmwIF3ER2sh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDWV3YhemWybHnjZZRqd25iYX\0[ZIhPDhiaILzJIJ6KHCuYYH1[UBie3OjeTygSWM2OD1yLkROwG0> MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl|MEG1Nkc,OjN7M{CxOVI9N2F-
MDCK MYfBcpRqfmm{YXygZZN{[Xl? MXm0PEBpenN? M2nk[WFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JIlv\my3ZX76ZUBCKH[rcoXzJGEwX1OQL{OzJEhJOE5zKTDpcoZm[3SnZDDpckBOTEONIHPlcIx{KGGodHXyJFQ5KGi{czDifUBxdGGzdXWgZZN{[XluIFXDOVA:OC52Nt88US=> NE\HdWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mzNFE2Oid-MkO5N|AyPTJ:L3G+
HL60 NUfYeHFzSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M4H5WFczKGi{cx?= MmfyRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKTE[wJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OC53NEVOwG0> MnLSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5Mke1OlkoRjJ7N{K3OVY6RC:jPh?=
HCT116 M2LpcGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MXq3NkBpenN? NHTnTYRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjDWFEyPiClZXzsd{BwfmW{LXX4dJJme3OrbnegSGhQTEhiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0xNjZ5Od88US=> NYLDS413RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3Nlc2PjlpPkK5O|I4PTZ7PD;hQi=>
Jurkat T MULBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MnLnO|IhcHK| NH\SRXRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFr1dotifCCWIHPlcIx{KGG|c3Xzd4VlKGG|IFTORUBkd262ZX70JIFnfGW{IEeyJIhzeyCkeTDIc4VkcHO2IEOzNlU5KGS7ZT3iZZNm\CCobIXvdoV{[2WwY3WgZZN{[XluIFnDOVA:OC57Md88US=> M3jaWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OUO5O|QzLz5{OUmzPVc1OjxxYU6=
Jurkat T MX\BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M4LhUlczKGi{cx?= NGG0bItCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFr1dotifCCWIHPlcIx{KGG|c3Xzd4VlKGG|IFTORUBkd262ZX70JIFnfGW{IEeyJIhzeyCkeTDIc4VkcHO2IEOzNlU5KGS7ZTDiZZNm\CCobIXvdo9u\XS{aXOgcYV1cG:mLDDJR|UxRTBwOUROwG0> NWPX[HVzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiyN|U4ODJpPkK4NlM2PzB{PD;hQi=>
MIAPaCa2 M1LkdGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MmXIO|IhcHK| MmLjRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPSVHQZWNiOiClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUGuOlnPxE1? MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd{N{W2PUc,Ojl5Mke1Olk9N2F-
Jurkat T NU\ySVh[S3m2b4TvfIlkcXS7IHHzd4F6 M4q0XlczKGi{cx?= MlXMR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTpVzc2G2IGSgZ4VtdHNiYYPz[ZN{\WRiYYOgZ49ueG:3bnSgZ49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDpcoR2[2VidYCgeI8hOzBnIHPlcIwh\GWjdHigZYZ1\XJiN{KgbJJ{KGK7IFjv[YNpe3RiM{OyOVgh\HmnIHLhd4VlKG[udX;yc41mfHKrYzDt[ZRpd2RuIFHjeIl3cXS7PUS1{txO NF\1UoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEKzOVcxOid-MkiyN|U4ODJ:L3G+
Jurkat T Ml\kR5l1d3SxeHnjbZR6KGG|c3H5 MoTJO|IhcHK| MnLlR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTpVzc2G2IGSgZ4VtdHNiYYPz[ZN{\WRiYYOgZ49ueG:3bnSgZ49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDpcoR2[2ViPU6zNEUh[2WubDDk[YF1cCCjZoTldkA4OiCqcoOgZpkhS2WubGTvfEBoemWnbjDhd5NigSxiQXP0bZZqfHl;NEiuNu69VQ>? M1rZPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OUO5O|QzLz5{OUmzPVc1OjxxYU6=
HCT116 MYrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1PYdFczKGi{cx?= MXfHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDIR3QyOTZiY3XscJMhd3[ncj3lfJBz\XO|aX7nJGRJV0SKIHH0JFIhfGmvZYOgZY51cXC{b3zp[oVz[XSrdnWgTWM2OCCjZoTldkA4OiCqcoOgbY4h[WK|ZX7j[UBw\iC3cnnkbY5mKGK7IF3UWEBie3OjeR?= MojHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5Mke1OlkoRjJ7N{K3OVY6RC:jPh?=
HCT116 M2XNPGN6fG:|dHH0bYMh[WO2aY\peJkh[XO|YYm= NVzVW4xzOTBidV2= MWWyOEBpenN? M2XXU2N6fG:|dHH0bYMh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINwdG:weTDmc5JucW6pIHH0JFExKHWPIHHmeIVzKDJ2IHjyd{BjgSClconzeIFtKH[rb3zleEB{fGGrbnnu[{Bj[XOnZDDhd5NigQ>? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd{N{W2PUc,Ojl5Mke1Olk9N2F-
HCT116 M3z4OWN6fG:|dHH0bYMh[WO2aY\peJkh[XO|YYm= MljYNVAhfU1? NV;6RYVRPyCmYYnz NXv0VYFHS3m2b4P0ZZRq[yCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJGhEXDFzNjDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h[2:ub375JIZwem2rbnegZZQhOTBidV2gZYZ1\XJiNzDkZZl{KGK7IHPyfZN1[WxidnnvcIV1KHO2YXnubY5oKGKjc3XkJIF{e2G7 MoS2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5Mke1OlkoRjJ7N{K3OVY6RC:jPh?=
U937 M{XjeGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NYLOOI5ZOC5yMTD0c{AyOCC3TR?= NHLJToM{KGSjeYO= MoTzRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDDSnNGNWyjYnXs[YQhcHWvYX6gWVk{PyClZXzsd{BifCByLkCxJJRwKDFyIIXNJIFnfGW{IEOg[IF6eyCkeTDwdo9xcWSrdX2gbY9lcWSnIIP0ZYlvcW6pLXLhd4VlKG[ub4egZ5l1d22ndIL5 MlvjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl7M{m3OFIoRjJ7OUO5O|QzRC:jPh?=
THP1 NXzub5JMSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NY\lUlhbOC5yMTD0c{AyOCC3TR?= MYqzJIRigXN? NXPDSI5ISW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBETlOHLXzhZoVt\WRiaIXtZY4hXEiSMTDj[YxteyCjdDCwMlAyKHSxIEGwJJVOKGGodHXyJFMh\GG7czDifUBxem:yaXTpeY0hcW:maXTlJJN1[WmwaX7nMYJie2WmIH\sc5ch[3m2b33leJJ6 NGPpdXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUmzPVc1Oid-Mkm5N|k4PDJ:L3G+
U937 NGfuR4FHfW6ldHnvckBie3OjeR?= M1OxN|AvOSC2bzCxNEB2VQ>? NWDNd3RIfXBidH:gOEBl[Xm| MoXGTY5lfWO2aX;uJI9nKG27ZXzvbYQh\GmoZnXy[Y51cWG2aX;uJIlvKGi3bXHuJHU6OzdiY3XscJMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gR2QyOWJiZYjwdoV{e2mxbjDs[ZZmdCCjdDCwMlEhfG9iMUCgeW0hfXBidH:gOEBl[Xm|IHL5JJBzd3CrZHn1cUBqd2SrZHWgd5RicW6rbnetZoF{\WRiZnzve{BkgXSxbXX0dpk> NXO2RWo3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm5N|k4PDJpPkK5PVM6PzR{PD;hQi=>
THP1 NWDUUYJXTnWwY4Tpc44h[XO|YYm= NHXqPFQxNjFidH:gNVAhfU1? NX;uSG05fXBidH:gOUBl[Xm| NF;CTllKdmS3Y4Tpc44hd2ZibYnlcI9q\CCmaX\m[ZJmdnSrYYTpc44hcW5iaIXtZY4hXEiSMTDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBETDFzYjDlfJBz\XO|aX;uJIxmfmWuIHH0JFAvOSC2bzCxNEB2VSC3cDD0c{A2KGSjeYOgZpkheHKxcHnkbZVuKGmxZHnk[UB{fGGrbnnu[{1j[XOnZDDmcI94KGO7dH;t[ZRzgQ>? M3Wwd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OUO5O|QzLz5{OUmzPVc1OjxxYU6=
THP1 MVfGeY5kfGmxbjDhd5NigQ>? NH;RblQxNjFidH:gNVAhfU1? NEXLR4p2eCC2bzC1JIRigXN? M3vRT2lv\HWldHnvckBw\iCveXXsc4llKGSrZn\ldoVvfGmjdHnvckBqdiCqdX3hckBVUFBzIHPlcIx{KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJGNFOTRiZYjwdoV{e2mxbjDs[ZZmdCCjdDCwMlEhfG9iMUCgeW0hfXBidH:gOUBl[Xm|IHL5JJBzd3CrZHn1cUBqd2SrZHWgd5RicW6rbnetZoF{\WRiZnzve{BkgXSxbXX0dpk> NU[5N3YxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm5N|k4PDJpPkK5PVM6PzR{PD;hQi=>

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 375.37
Formula

C23H15F2NO2

CAS No. 96187-53-0
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(C2=C(C=CC(=C2)F)N=C1C3=CC=C(C=C3)C4=CC=CC=C4F)C(=O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03760666 Completed Drug: Brequinar|Drug: Brequinar + Ribavirin Acute Myeloid Leukemia Clear Creek Bio Inc. November 26 2018 Phase 1|Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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