Pirfenidone (S-7701)

For research use only.

Catalog No.S2907 Synonyms: AMR-69

33 publications

Pirfenidone (S-7701) Chemical Structure

CAS No. 53179-13-8

Pirfenidone (S-7701, AMR-69) is an inhibitor for TGF-β production and TGF-β stimulated collagen production, reduces production of TNF-α and IL-1β, and also has anti-fibrotic and anti-inflammatory properties. Pirfenidone attenuates chemokine (CC motif) ligand-2 (CCL2) and CCL12 production with anti-fibrotic activity. Phase 3.

Size Price Stock Quantity  
10mM (1mL in DMSO) RMB 731.52 In stock
RMB 568.87 In stock
RMB 2218.54 In stock
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Selleck's Pirfenidone (S-7701) has been cited by 33 publications

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Biological Activity

Description Pirfenidone (S-7701, AMR-69) is an inhibitor for TGF-β production and TGF-β stimulated collagen production, reduces production of TNF-α and IL-1β, and also has anti-fibrotic and anti-inflammatory properties. Pirfenidone attenuates chemokine (CC motif) ligand-2 (CCL2) and CCL12 production with anti-fibrotic activity. Phase 3.
TGF-β [2]
(Cell-free assay)
In vitro

Pirfenidone (< 300 μg/mL) suppresses the proinflammatory cytokine tumor necrosis factor-α (TNF-α) by a translational mechanism in RAW264.7 cells, which is independent of activation of the mitogen-activated protain kinase (MAPK) 2, p38 MAP kinase, and c-Jun N-terminal kinase (JNK). [1] Pirfenidone (< 10 mM) leads to reduced glioma cell density in concentration-dependent manner in LN-18, T98G, LNT-229 and LN-308 cell lines. Pirfenidone (< 5 mM) reduces TGF-β bioactivity by affecting TGF-β2 mRNA expression and processing of pro-TGF-β in CCL-64 cells. Pirfenidone (< 8.3 mM) inhibits the activity of recombinant furin and downregulates the expression of MMP-11 in a dose-dependent manner in LN-308 cells. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HFL1 cells NH3pdXBHfW6ldHnvckBie3OjeR?= NIXSOmU1QCCq MmXsRY51cW[rYoLveIlkKGGldHn2bZR6KGmwIFjGUFEh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBie3OjeTygTWM2OCB;IECuO|Eh|ryPLh?= MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjh7NkewO{c,OjZ6OU[3NFc9N2F-
LNCaP NGrQ[FBE\WyuIHfyc5d1cCCjc4PhfS=> NXvsbnV3OCxiMD6xMEAxNjNibXevcYw> NGrGXo8{KGSjeYO= NX;yUVdSWE[GIITy[YF1dWWwdDDzbYdvcW[rY3HueIx6KHO3cIDy[ZN{\WRidHjlJIdzd3e2aDDv[kBk\WyuczDheEAxNjNibXevcYw> NFjpOIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|ME[yNVE4PSd-M{C2NlEyPzV:L3G+
PC-3 MVLD[YxtKGe{b4f0bEBie3OjeR?= MW[wMEAxNjFuIECuN{Bu\y:vbB?= NXfyOVc3OyCmYYnz Mn[2VGZFKHS{ZXH0cYVvfCC|aXfubYZq[2GwdHz5JJN2eHC{ZYPz[YQhfGinIHfyc5d1cCCxZjDj[YxteyCjdDCwMlMhdWdxbXy= Mn\sQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB4MkGxO|UoRjNyNkKxNVc2RC:jPh?=
E9 MUjD[YxtKGe{b4f0bEBie3OjeR?= M3;2[|AtKDBwMTygNE4{KG2pL33s M3PxS|Mh\GG7cx?= NUDOV3E3WE[GIITy[YF1dWWwdDDzbYdvcW[rY3HueIx6KHO3cIDy[ZN{\WRidHjlJIdzd3e2aDDv[kBk\WyuczDheEAxNjNibXevcYw> NIC1R4g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|ME[yNVE4PSd-M{C2NlEyPzV:L3G+
F10 NEjGUZBE\WyuIHfyc5d1cCCjc4PhfS=> MXWwMEAxNjFuIECuN{Bu\y:vbB?= MmPtN{Bl[Xm| M3S5VHBHTCC2cnXheI1mdnRic3nncolncWOjboTsfUB{fXCycnXzd4VlKHSqZTDndo94fGhib3[gZ4VtdHNiYYSgNE4{KG2pL33s MkfuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB4MkGxO|UoRjNyNkKxNVc2RC:jPh?=
AIDL NEPKeppE\WyuIHfyc5d1cCCjc4PhfS=> NFfaT3MxNCByLkGsJFAvOyCvZz;tcC=> MVezJIRigXN? MkW0VGZFKHS{ZXH0cYVvfCC|aXfubYZq[2GwdHz5JJN2eHC{ZYPz[YQhfGinIHfyc5d1cCCxZjDj[YxteyCjdDCwMlMhdWdxbXy= NIjWZXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|ME[yNVE4PSd-M{C2NlEyPzV:L3G+
human intestinal fibroblasts (HIFs) MWTGeY5kfGmxbjDhd5NigQ>? NWPhcmxIOSCq M2XyTHBHTCCrbnjpZol1eyCKSV\zJIRq\m[ncnXueIlifGmxbjDhcoQhfGinIHPvcIxi\2WwIHTldI9{cXSrb36gbY5lfWOnZDDifUBVT0ZvzsKxMi=> MmrJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzBzNUK4OFgoRjNyMUWyPFQ5RC:jPh?=

... Click to View More Cell Line Experimental Data

Methods Test Index PMID

PubMed: 22132230     

Effects of 1.0 or 1.5 mg/ml pirfenidone on α-SMA expression and morphological changes of CFs (Cardiac fibroblasts) were determined by immunofluorescence staining with anti-α-SMA antibody. Scale bar = 50.0 µm. 

SMAD4 / SMAD2 / SMAD3 ; 

PubMed: 23437252     

After stimulated by 12.5 ng/ml TGF-β2 for 24 hours, the staining of nuclei SMAD4 (green) enhanced when that of SMAD4 in the cytoplasm were weakened, and SMAD2/3 proteins (red) were assembled on the nuclear membrane and some of them entered the nuclei. Expression of nuclei SMAD2/3 and SMAD4 were depressed after treatment with 0.5 mg/ml pirfenidone. The nuclei stained by DAPI (blue). Magnification, ×1000.

F-actin / β-catenin / Vimentin ; 

PubMed: 29325547     

Immunofluorescence staining showed that treatment with SB or PFD increased E-cadherin levels, decreased vimentin and nuclear β-catenin distribution in MCF-7 and MDA-MB-231 cells. CAFs promoted F-actin polymerization and stability in cancer cells, whereas treatment with SB or PFD impaired the formation of stress fibers. Scale bar: 15 μm

22132230 23437252 29325547
In vivo Pirfenidone (250 mg/kg) potently inhibits the production of the proinflammatory cytokines, TNF-alpha, interferon-gamma, and interleukin-6, but enhances the production of the anti-inflammatory cytokine, interleukin-10, in mice. [1] Pirfenidone (250 mg/kg/day) ameliorates cyclosporine-induced fibrosis by about 50% and decreases TGF-beta1 protein expression by 80% in Sprague-Dawley rats receiving a low-salt diet. [3] Pirfenidone (400 mg/kg/day) inhibits heat shock protein 47-positive cells and myofibroblasts, the principal cells responsible for the accumulation and deposition of extracellular matrix seen in pulmonary fibrosis in ICR mice intravenously injected with bleomycin. [4] Pirfenidone (0.5%, liquid diet) treatment reduces the degree of liver injury in rats, as determined by alanine aminotransferase values and necro-inflammatory score, which is associated with reduced hepatic stellate cells proliferation and collagen deposition. Pirfenidone (0.5%, liquid diet) administration downregulates dimethylnitrosamine induced transcripts levels of procollagen alpha1(I), TIMP-1 and MMP-2 by 50-60% in rats, and this is associated with a 70% reduction in collagen deposition. [5]


Animal Research:[3]
- Collapse
  • Animal Models: Sprague-Dawley rats receiving a low-salt diet
  • Dosages: 250 mg/kg
  • Administration: oral diet
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 37 mg/mL (199.76 mM)
Water Insoluble
Ethanol '37 mg/mL
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 185.22


CAS No. 53179-13-8
Storage powder
in solvent
Synonyms AMR-69
Smiles CC1=CN(C(=O)C=C1)C2=CC=CC=C2

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04607928 Recruiting Drug: Pirfenidone|Drug: Placebo Fibrotic Pulmonary Sequelae Post-COVID19 Infection Institut d''Investigació Biomèdica de Bellvitge August 1 2020 Phase 2
NCT04126538 Recruiting Drug: pirfenidone capsule Renal Insufficiency Beijing Continent Pharmaceutical Co Ltd. August 27 2019 Phase 1
NCT03359863 Active not recruiting Drug: Pirfenidone Restrictive Chronic Lung Allograft Dysfunction|Lung Transplant Rejection University of California San Francisco|Genentech Inc. March 7 2018 Phase 2
NCT02689778 Active not recruiting Drug: Pirfenidone|Drug: Placebo Diabetic Nephropathy|Albuminuria Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran|Grupo Medifarma S. A. de C. V. March 2016 Phase 3
NCT02598193 Completed Drug: Nintedanib|Drug: Pirfenidone Idiopathic Pulmonary Fibrosis Hoffmann-La Roche January 14 2016 Phase 4
NCT02579603 Completed Drug: Nintedanib|Drug: Pirfenidone Idiopathic Pulmonary Fibrosis Boehringer Ingelheim October 16 2015 Phase 4

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Frequently Asked Questions

  • Question 1:

    I'd like to know how to use your 50mg dose pirfenidone to make a solution for intraperitoneal injection in a mouse. Could you give me some advice?

  • Answer:

    For I.P. injection, S2907 Pirfenidone can be dissolved in 2% DMSO+30% PEG 300+ddH2O at 10 mg/ml clearly. When preparing the solution, please dissolve the compound in DMSO clearly first. Then add PEG, after they mixed well, then dilute with water.

TGF-beta/Smad Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID