Kartogenin

For research use only.

Catalog No.S7658 Synonyms: KGN

4 publications

Kartogenin Chemical Structure

CAS No. 4727-31-5

Kartogenin (KGN) is an activator of the smad4/smad5 pathway, and promotes the selective differentiation of multipotent mesenchymal stem cells into chondrocytes.

Selleck's Kartogenin has been cited by 4 publications

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Biological Activity

Description Kartogenin (KGN) is an activator of the smad4/smad5 pathway, and promotes the selective differentiation of multipotent mesenchymal stem cells into chondrocytes.
Targets
Smad4 [1]
()
Smad5 [1]
()
In vitro

Kartogenin induces chondrocyte differentiation from hMSCs by enhancing the nuclear localization of CBFβ, and protects articular chondrocytes. [1] Kartogenin promotes the type-I collagen synthesis of dermal fibroblasts via activation of the smad4/smad5 pathway without obvious cytotoxicity on fibroblasts’ viability, morphology and survival. [2]

In vivo Kartogenin promotes cartilage repair in collagenase VII (10 μM)– and surgery (100 μM)-induced OA models in mice. [1] Kartogenin (100 nM) also stimulats collagen synthesis in the mouse dermis. [2] In addition, Kartogenin stimulates mouse limb skeletal growth and synovial joint development. [3]

Protocol

Cell Research:[2]
- Collapse
  • Cell lines: Fibroblasts
  • Concentrations: ~1 μM
  • Incubation Time: 48 hours
  • Method: For the cell viability assay, fibroblasts are seeded on six-well plates, followed by Kartogenin or DMSO treatment. According to the manufacturer’s instructions, cell viability and viability are assessed by AlamarBlue assay.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: Collagenase VII-induced OA mouse model and Surgery-induced OA mouse model
  • Dosages: 10 μM or 100 μM
  • Administration: Intra-articular injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 63 mg/mL (198.52 mM)
Water Insoluble
Ethanol '5 mg/mL warmed
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+40% PEG 300+2% Tween80+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 317.34
Formula

C20H15NO3

CAS No. 4727-31-5
Storage powder
in solvent
Synonyms KGN
Smiles C1=CC=C(C=C1)C2=CC=C(C=C2)NC(=O)C3=CC=CC=C3C(=O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID