Molecular Weight(MW): 489.99
SIS3, a novel specific inhibitor of Smad3, inhibits TGF-β and activin signaling by suppressing Smad3 phosphorylation without affecting the MAPK/p38, ERK, or PI3-kinase signaling pathways.
Cited by 11 Publications
5 Customer Reviews
Cultured cardiac fibroblasts infected with vector lentiviruses or EphrinB2 overexpressing lentiviruses were stimulated with specific antagonist against Stat3 (Stattic, 2.5μM) and/or Smad3 (SIS3, 1μM), respectively. The expression level of α-SMA was quantified via western blotting. In vitro experiments repeat 3 times, *P<0.05, **P<0.01.
Circ Res, 2017, 121(6):617-627. SIS3 HCl purchased from Selleck.
Effect of SIS3, Smad3 inhibitor on TMAO-induced Cardiac Hypertrophy. SIS3 (3 μM) was used to treat cardiomyocytes in the presence or absence of TMAO for 72 h. The cell areas were detected by an immunofluorescence assay in neonatal mouse cardiomyocytes (Scale bar: 25 µm).
Lab Invest, 2018, doi: 10.1038/s41374-018-0091-y. SIS3 HCl purchased from Selleck.
SIS3 alleviates BLM-induced lung fibrosis. Mice were intratracheally challenged with BLM (1.5 mg/kg) or saline on day 0, and with either SIS3 or control vehicle by intraperitoneal injection (2 mg/kg) every other day until the end of the experiment. Mice were sacrificed three weeks after BLM challenge. (A) Lung morphology of mice in each group was evaluated by hematoxylin-eosin staining. (B) Collagen fiber deposition in pulmonary interstitium was evaluated by Masson's staining in lungs in rats of each group. Ashcroft score (C) and hydroxyproline content (D) on day 21 after BLM administration. Results are expressed as means ±SD (n ¼ 5). Statistical analysis of indicated columns was performed using Student's t-test. **P < 0.01. ***P < 0.001.
Biochem Biophys Res Commun, 2018, 503(2):757-762. SIS3 HCl purchased from Selleck.
Fibroblasts were pretreated with SIS3 for 1 hour and then incubated with 10 ng/mL TGF-b1 for 24 hours. Whole-cell lysates were examined by Western blot analysis. TGF-b1-induced Tnc expression was significantly abrogated by SIS3 (SIS3 diluted in DMSO, 3 μM) (***P <.001, **P <.01). Tnc = tenascin-C.
Laryngoscope, 2018, doi: 10.1002/lary.27164. SIS3 HCl purchased from Selleck.
Purity & Quality Control
Choose Selective TGF-beta/Smad Inhibitors
|Description||SIS3, a novel specific inhibitor of Smad3, inhibits TGF-β and activin signaling by suppressing Smad3 phosphorylation without affecting the MAPK/p38, ERK, or PI3-kinase signaling pathways.|
Addition of SIS3 attenuates the effects of TGF-β1 by reducing the transcriptional activity. SIS3 also inhibits the myofibroblast differentiation of fibroblasts by TGF-β1. SIS3 completely diminishes the constitutive phosphorylation of Smad3 as well as the up-regulated type I collagen expression in scleroderma fibroblasts, thus abolishes the ECM overexpression in the TGF-β1-treated normal dermal fibroblasts and scleroderma fibroblasts in vitro.
|In vivo||SIS3 inhibits Smad3 activation in streptozotocin(STZ)-induced diabetic nephropathy in Tie2-Cre;Loxp-EGFP mice. It also reduces AGE-induced EndoMT and decreases EndoMT in STZ-induced diabetic nephropathy in Tie2-Cre;Loxp-EGFP mice. SIS3 significantly reduces collagen IV and fibronectin expression in the glomeruli and tubulointerstitium of STZ-injected Tie2-Cre;Loxp-EGFP mice, suggesting that SIS3 retards the early development of STZ-induced diabetic glomerulosclerosis and tubulointerstitial fibrosis. However, SIS3 administration does not reduce proteinuria.|
|In vitro||DMSO||97 mg/mL (197.96 mM)|
|Ethanol||24 mg/mL (48.98 mM)|
|In vivo||Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
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Clinical Trial Information
|NCT Number||Recruitment||interventions||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT03884621||Recruiting||Other: EHP Group||Stroke||The Hong Kong Polytechnic University||September 25 2018||Not Applicable|
|NCT02726685||Not yet recruiting||Other: respiratory training.|Other: sham training.||Stroke||Chung Shan Medical University||August 1 2018||Not Applicable|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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