SIS3 HCl

For research use only.

Catalog No.S7959

33 publications

SIS3 HCl Chemical Structure

Molecular Weight(MW): 489.99

SIS3, a novel specific inhibitor of Smad3, inhibits TGF-β and activin signaling by suppressing Smad3 phosphorylation without affecting the MAPK/p38, ERK, or PI3-kinase signaling pathways.

Size Price Stock Quantity  
USD 97 In stock
USD 147 In stock
USD 447 In stock
Bulk Discount
Bulk Inquiry

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's SIS3 HCl has been cited by 33 publications

Purity & Quality Control

Choose Selective TGF-beta/Smad Inhibitors

Biological Activity

Description SIS3, a novel specific inhibitor of Smad3, inhibits TGF-β and activin signaling by suppressing Smad3 phosphorylation without affecting the MAPK/p38, ERK, or PI3-kinase signaling pathways.
Targets
Smad3 [1]
()
In vitro

Addition of SIS3 attenuates the effects of TGF-β1 by reducing the transcriptional activity. SIS3 also inhibits the myofibroblast differentiation of fibroblasts by TGF-β1. SIS3 completely diminishes the constitutive phosphorylation of Smad3 as well as the up-regulated type I collagen expression in scleroderma fibroblasts, thus abolishes the ECM overexpression in the TGF-β1-treated normal dermal fibroblasts and scleroderma fibroblasts in vitro[1].
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HK-2 cell MkPhR4VtdCC4aXHibYxqfHliYYPzZZk> M2DMflXjiIoQvF2= MXKxJIg> NUnZXGRRXHKnYYTt[Y51KHerdHigV2lUOyC|dYDwdoV{e2WmIHPh[I1qfW1vaX7keYNm\CCKSz2yJINmdGxiZHXheIgv MWKzNFgxPDR5MB?=
A549 cells MWHGeY5kfGmxbjDhd5NigQ>? NEW2N5k{KM7:TR?= MoXPWIhmKGOnbHzzJIlvKHSqZTDTTXM{KGe{b4XwJIRqe3CuYYnl[EBiKHOyaX7kcIUue2ijcHXkJIVtd26pYYTl[EBncWK{b3LsZZN1NWyra3WgcY9zeGixbH;nfU4> NXHvSIQ1Ojl{MEewOVU>
AML12 cell line NIDBVo5HfW6ldHnvckBie3OjeR?= NH3teWEyOCEQvF2= MlLoN|AhdWmwcx?= NF7BTmFUUVN|IHLsc4NseyCWR1[t{tIudWWmaXH0[YQheGixc4Doc5J6dGG2aX;uJI9nKFOPQVSzJIFv\CC2aHWgeI91[WxiU13BSFIwOyCuZY\lcJMh[XKnIIXuZ4hidmenZD6= NYjXfmhVOjd2NkKwO|U>
A549 cells MYHGeY5kfGmxbjDhd5NigQ>? M{jwcVMh|ryP M3nTcVQhcA>? M2riZXNKWzNic3nncolncWOjboTsfUBz\XO2b4Ll[EBGNWOjZHjldolvKGW6cILld5Nqd25iYX7kJIlueGGrcnXkJJZqdWWwdHnuJIFv\CCVbnHpcEBmgHC{ZYPzbY9vKGmwIITo[UBxemW|ZX7j[UBw\iCWR1[t{tIyNg>? M3XxXlI1PTd|MEO4
MCF-7 cells NH\KXnNRem:uaX\ldoF1cW:wIHHzd4F6 M3LVflIvPSEQvF2= M2DwPINwdmO3coLlcpQhfHKnYYTt[Y51KHerdHigV2lUOyC{ZXT1Z4VlKHSqZTDpcohq[mm2b4L5JIVn\mWldDDv[kBmdGyjZ3njJIFkcWRib36geIhmKHC{b3zp[oVz[XSrb36gc4YhVUOILUegZpJm[XO2IHPhcoNmeiClZXzsd{4> MXqyOFUzQDB|OB?=

... Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Western blot
COX-2 / HMGB1; 

PubMed: 24098479     


SB431542 (10 µM) or SIS3 (30 µM) blocked TGF-β1-induced COX-2 downregulation. Cells were pretreated for 60 min with the inhibitor, and then incubated with or without TGF-β1 (5 ng/mL) for 24 h. 

p-Smad3 / Smad3 / E-cadherin / Ck18 / Fibronectin / MMP9; 

PubMed: 23430956     


Representative western blots of E-cadherin, CK18, fibronectin and MMP-9 in HepG2 and BEL7402 cells with or without SIS3.

24098479 23430956
Immunofluorescence
KDEL / Fibronectin; 

PubMed: 29743238     


Inhibition of TGFβ signaling blocks Dex-induced intracellular fibronectin co-localization with ER stress markers in primary human TM cells. Primary human TM cells (n = 3 cell strains) were treated with Veh or Dex in the presence or absence of TGFβ-signaling inhibitors (LY364947 and SIS3) for 72 h, and cells were stained for fibronectin and KDEL (Triton-permeabilized cells). The Dex-induced intracellular fibronectin load and its co-localization with KDEL were completely blocked by TGFβ-signaling inhibitors. Scale bar = 50 micron.

α-SMA / Vimentin; 

PubMed: 25793924     


Effect of LY364947 and SIS3 on the expression of EMT markers altered by TGFβ1, seen by Immunofluorescence.

p-Smad3; 

PubMed: 29207055     


Localization of p-Smad3 occurs in the cells as shown by immunofluorescence. p-Smad3 is shown in red, TRITC-stained; counterstaining of nuclei with DAPI is shown in blue. Scale bar, 50 μm. The cell groups were as follows: control, cells cultured in serum-free DMEM; TGF-β1, cells cultured in serum-free DMEM and exposed to 5 ng/ml TGF-β1; SIS3, cells were treated with 3 μM SIS3 (specific inhibitor of Smad3) which was added 4 h prior to 5 ng/ml TGF-β1 exposure. TRITC, tetramethylrhodamine isothiocyanate; DAPI, 4′,6-diamidino-2-phenylindole.

E-cadherin / α-SMA ; 

PubMed: 29207055     


Co-localization of E-cad and α-SMA occurs in A549 cells as determined by immunofluorescent staining. E-cad (green) was present in the cytoplasm, FITC-stained; α-SMA (red) was present in the cytoplasm, TRITC-stained. Cell nuclei were counterstained with DAPI (blue). Scale bar, 40 μm. The cell groups were as follows: control, cells cultured in serum-free DMEM; TGF-β1, cells cultured in serum-free DMEM and exposed to 5 ng/ml TGF-β1; SIS3, cells were treated with 3 μM SIS3 (specific inhibitor of Smad3) which was added 4 h prior to 5 ng/ml TGF-β1 exposure; BMSCs-CM, BMSCs-CM was added prior to 5 ng/ ml TGF-β1 exposure. E-cad, E-cadherin (E-calcium mucins); α-SMA, α-smooth muscle actin; TRITC, tetramethyl rhodamine isothiocyanate; FITC, fluorescein isothiocyanate; DAPI, 4′,6-diamidino-2-phenylindole.

29743238 25793924 29207055
In vivo SIS3 inhibits Smad3 activation in streptozotocin(STZ)-induced diabetic nephropathy in Tie2-Cre;Loxp-EGFP mice. It also reduces AGE-induced EndoMT and decreases EndoMT in STZ-induced diabetic nephropathy in Tie2-Cre;Loxp-EGFP mice. SIS3 significantly reduces collagen IV and fibronectin expression in the glomeruli and tubulointerstitium of STZ-injected Tie2-Cre;Loxp-EGFP mice, suggesting that SIS3 retards the early development of STZ-induced diabetic glomerulosclerosis and tubulointerstitial fibrosis. However, SIS3 administration does not reduce proteinuria[2].

Protocol

Cell Research:

[1]
- Collapse
  • Cell lines: Normal human dermal fibroblasts, Scleroderma fibroblasts
  • Concentrations: 0.3, 1, 3 μM
  • Incubation Time: 72 h
  • Method:

    Normal human dermal fibroblasts are plated at a density of 105 cells/well in six-well culture plates and grown until subconfluence in MEM containing 10% FCS. Cells are quiesced by 24-h incubation in serum-free MEM, followed by incubation in serum-free medium in the presence or absence of SIS3 before the collection of cells for 72 h. Then, the cells are detached from the wells by trypsin treatment and counted using a Coulter counter.


    (Only for Reference)
Animal Research:

[2]
- Collapse
  • Animal Models: Male C57BL/6J mice
  • Dosages: 1, 2.5, or 5 μg/g
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 97 mg/mL (197.96 mM)
Ethanol 24 mg/mL (48.98 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing. 		5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 489.99
Formula

C28H27N3O3.HCl
CAS No. 521984-48-5
Storage powder
in solvent
Synonyms N/A
Smiles CN1C(=C(C2=C1N=CC=C2)C=CC(=O)N3CCC4=CC(=C(C=C4C3)OC)OC)C5=CC=CC=C5.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03884621 Recruiting Other: EHP Group Stroke The Hong Kong Polytechnic University September 25 2018 Not Applicable
NCT02726685 Unknown status Other: respiratory training.|Other: sham training. Stroke Chung Shan Medical University August 1 2018 Not Applicable

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field
selleck catalog

TGF-beta/Smad Signaling Pathway Map

Related TGF-beta/Smad Products

  • SD-208 New

    SD-208 is a selective TGF-βRI (ALK5) inhibitor with IC50 of 48 nM, >100-fold selectivity over TGF-βRII.

  • LDN-214117 New

    LDN-214117 is a potent and selective BMP type I receptor kinase ALK2 inhibitor with IC50 of 24 nM.

  • SB431542

    SB431542 is a potent and selective inhibitor of ALK5 with IC50 of 94 nM in a cell-free assay, 100-fold more selective for ALK5 than p38 MAPK and other kinases.

  • LDN-193189

    LDN-193189 is a selective BMP signaling inhibitor, inhibits the transcriptional activity of the BMP type I receptors ALK2 and ALK3 with IC50 of 5 nM and 30 nM in C2C12 cells, respectively, exhibits 200-fold selectivity for BMP versus TGF-β.

  • Galunisertib (LY2157299)

    Galunisertib (LY2157299) is a potent TGFβ receptor I (TβRI) inhibitor with IC50 of 56 nM in a cell-free assay. Phase 2/3.

  • SB525334

    SB525334 is a potent and selective inhibitor of TGFβ receptor I (ALK5) with IC50 of 14.3 nM in a cell-free assay, 4-fold less potent to ALK4 than ALK5 and inactive to ALK2, 3, and 6.

  • LY2109761

    LY2109761 is a novel selective TGF-β receptor type I/II (TβRI/II) dual inhibitor with Ki of 38 nM and 300 nM in a cell-free assay, respectively; shown to negatively affect the phosphorylation of Smad2. LY2109761 blocks autophagy and induces apoptosis.

  • RepSox

    RepSox is a potent and selective inhibitor of the TGFβR-1/ALK5 with IC50 of 23 nM and 4 nM for ATP binding to ALK5 and ALK5 autophosphorylation in cell-free assays, respectively.

  • Pirfenidone

    Pirfenidone (S-7701, AMR-69) is an inhibitor for TGF-β production and TGF-β stimulated collagen production, reduces production of TNF-α and IL-1β, and also has anti-fibrotic and anti-inflammatory properties. Pirfenidone attenuates chemokine (CC motif) ligand-2 (CCL2) and CCL12 production with anti-fibrotic activity. Phase 3.

Tags: buy SIS3|SIS3 ic50|SIS3 price|SIS3 cost|SIS3 solubility dmso|SIS3 purchase|SIS3 manufacturer|SIS3 research buy|SIS3 order|SIS3 mouse|SIS3 chemical structure|SIS3 mw|SIS3 molecular weight|SIS3 datasheet|SIS3 supplier|SIS3 in vitro|SIS3 cell line|SIS3 concentration|SIS3 nmr|SIS3 in vivo|SIS3 clinical trial|SIS3 inhibitor|SIS3 TGF-beta/Smad inhibitor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID