Alantolactone

Name: Alantolactone
Brand: Selleck Chemicals
SKU: S8318
Rating: 5 (1 reviews)

For research use only.

Catalog No.S8318 Synonyms: helenin, helenine, Eupatal

1 publication

CAS No. 546-43-0

Alantolactone (helenin, helenine, Eupatal), a naturally occurring eudesmane-type sesquiterpene lactone (SL), could induce activin/SMAD3 signaling and disrupt Cripto-1/activin receptor type II A interaction.

Selleck's Alantolactone has been cited by 1 publication

Onco Targets Ther, 2017, 10:1767-1776

PubMed: 28367059 ( click the link to review the publication )

1 Customer Review

Colon cancer cell lines (A) SW620, (B) HCT116, and (C) HT29 were treated with different concentrations (60, 12, 2.4, 0.48, and 0.098 μM) of nifuroxazide (nifu), niclosamide (nicl), cryptotanshinone (cry), and a lantolactone (ala), respectively, for 72 h. Cell proliferation in each group was detected by MTT assay. (D) HCT116, SW620, and HT29 cell lines were suppressed with niclosamide treatment at different concentrations (20, 10, 5, 2.5, and 1.25 μM) for 72 h. Subsequently, cell proliferation in each group was detected by MTT assay. The data were obtained from 3 independent experiments.

Onco Targets Ther, 2017, 10:1767-1776. Alantolactone purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description

Targets

STAT3 ^[1]
()

activin/SMAD3 ^[3]
()

In vitro

Alantolactone selectively suppresses STAT3 activation and exhibits potent anticancer activity in MDA-MB-231 cells. Alantolactone effectively suppressed both constitutive and inducible STAT3 activation at tyrosine 705. It decreased STAT3 translocation to the nucleus, its DNA-binding, and STAT3 target gene expression. Alantolactone significantly inhibits STAT3 activation with a marginal effect on MAPKs and on NF-κB transcription; however, this effect is not mediated by inhibiting STAT3 upstream kinases. SHP-1, SHP-2, and PTEN, which are protein tyrosine phosphatases (PTPs) were not affected by alantolactone. Alantolactone treatment resulted in the inhibition of migration, invasion, adhesion, and colony formation^[1]. Alantolactone could induce activin signaling and activin target gene expression. Alantolactone activated the activin signaling pathway in a cancer cell line. It could promote SMAD2/3 nuclear translocation in a very short time^[3].

In vivo

In vivo administration of alantolactone inhibited the growth of human breast xenograft tumors^[1]. Alantolactone exerts no toxic effect on liver and kidneys in vivo, The drug was well tolerated by mice and no mortality or any sign of pharmacotoxicity was found at a dose of 100 mg/kg during all the experimental periods. Alantolactone can cross blood-brain barrier^[2].

Protocol

Cell Research:

^[1]

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Cell lines: MDA-MB-231 and MCF-7 cells
Concentrations: 15 μM
Incubation Time: 24 h
Method:
Cell viability was evaluated using MTT assay. The cells were seeded into 96-well plates and maintained at 37 °C for 24 h. The cells were treated with alantolactone for 24 h. The MTT solution (0.5 mg/mL) was added to each well, and the cells were incubated for another 3 h. The MTT formazan crystals were dissolved in DMSO. The results were obtained by measuring the absorbance at a wavelength of 540 nm using a microplate reader.

(Only for Reference)

Animal Research:

^[1]

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Animal Models: athymic BALB/c nude mice
Dosages: 2.5 mg/kg
Administration: i.p.
(Only for Reference)

References

Solubility (25°C)

In vitro	DMSO	46 mg/mL (198.0 mM)
	Ethanol	46 mg/mL (198.0 mM)
	Water	Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Alantolactone SDF

Molecular Weight	232.32
Formula	C₁₅H₂₀O₂
CAS No.	546-43-0
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	helenin, helenine, Eupatal
Smiles	CC1CCCC2(C1=CC3C(C2)OC(=O)C3=C)C

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% DMSO+ % + % Tween 80+ % ddH₂O

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Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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The Serial Dilution Calculator Equation

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Alantolactone