For research use only.
Catalog No.S7223 Synonyms: E-616452, SJN 2511
Molecular Weight(MW): 287.32
RepSox is a potent and selective inhibitor of the TGFβR-1/ALK5 with IC50 of 23 nM and 4 nM for ATP binding to ALK5 and ALK5 autophosphorylation in cell-free assays, respectively.
Selleck's RepSox has been cited by 13 publications
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(C) Generation of pluripotent stem cell from Yamanaka factors-induced OG2-MEFs under treatment of diverse chemical compounds. Images of GFP+ colonies were taken on day 10 post-infection. VPA, 0.5 mmol/L. CHIR99021, 3 μmol/L. Repsox, 1 μmol/L. (D) Quantification of GFP+ colonies in (C). All figures are representative of three independent experiments (n = 3). All data are presented as mean ± SD. *P < 0.05, **P < 0.01 vs. DMSO
Protein Cell, 2017, 8(4):273-283. RepSox purchased from Selleck.
(a) Subcellular localization of Smad2/3 in HEKs grown in 3T3-J2 co-culture treated with dimethyl sulfoxide (DMSO), as a control, or 1 μM RepSox for 2 hrs (top panels). Lower panels represent corresponding nuclear counterstaining with Hoechst 33342. Bar=10 μm. (b) Nuclear-to-cytoplasmic ratios of Smad2/3 in HEKs as determined by fluorescence intensity using NIH ImageJ. Data shown are mean±SEM (n=6). ***P<0.001. (c) Proliferation of CK5+ epidermal cells in 3T3-J2 co-culture at day 5 as determined by a short-pulse labeling with BrdU, followed by quantification by flowcytometric analysis. Data shown are mean±SEM (n=3). **P<0.01.
Wound Repair Regen, 2017, 25(3):526-531. RepSox purchased from Selleck.
Late Alk4/5/7 activity is required for skeletogenesis in S. purpuratus and L. variegatus. DIC (A-G) and polari ed light (A’-G’) images of S. purpuratus embryos: a control embryo (A, A’) and an embryo exposed to 0.5 μM (B, B’) and 5 μM (C, C’) SB431542, 1 μM (D, D’) and 10 μM (E, E’) SB525334, and 0.1 μM (F, F’) and 1 μM (G, G’) RepSox from the mesenchyme blastula stage (24 hpf) to the prism stage (50 hpf). DIC (H-K) and polarized light (H’-K’) images of L. variegatus embryos: a control embryo (H, H’) and an embryo exposed to 10 μM (I, I’) SB431542, 40 μM (J, J’) SB525334, and 1 μM (K, K’) RepSox from the early gastrula stage to the pluteus stage. The white arrow indicates an anterolateral rod. CB = ciliary band, CP = coelomic pouch, G = tri-partite gut, PC = pigment cell.
Dev Biol, 2017,421(2):149-160. RepSox purchased from Selleck.
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Choose Selective TGF-beta/Smad Inhibitors
|Description||RepSox is a potent and selective inhibitor of the TGFβR-1/ALK5 with IC50 of 23 nM and 4 nM for ATP binding to ALK5 and ALK5 autophosphorylation in cell-free assays, respectively.|
RepSox inhibits ATP binding to ALK5 and ALK5 autophosphorylation with IC50 of 23 nM and 4 nM, respectively. RepSox inhibits TGF-β induced cellular PAI-1 luciferase activity with IC50 of 18 nM.  RepSox is able to successfully replace Sox2 in reprogramming by inhibiting transforming growth factor-β (Tgf-β) signaling, which in turn induces Nanog expression. Effect of RepSox inducing reprogramming does not require chromatin remodeling. RepSox is found to be efficient at generating iPSCs. 
|In vivo||RepSox is able to be contribute to forming chimeric embryos in vivo when injected into blastocysts. A one-day treatment with RepSox is sufficient to replace transgenic Sox2. |
|In vitro||DMSO||57 mg/mL (198.38 mM)|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
|Synonyms||E-616452, SJN 2511|
In vivo Formulation Calculator (Clear solution)
|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
|Dosage||mg/kg||Average weight of animals||g||Dosing volume per animal||ul||Number of animals|
|Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)|
|% DMSO % % Tween 80 % ddH2O|
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
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Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).
Molecular Weight Calculator
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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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