K02288

Catalog No.S7359

For research use only.

K02288 is a potent, and selective type I BMP receptor inhibitor with IC50 of 1.1, 1.8, 6.4 nM for ALK2, ALK1 and ALK6, showing weaker inhibition on other ALKs (3, 4, 5) and ActRIIA.

K02288 Chemical Structure

CAS No. 1431985-92-0

Selleck's K02288 has been cited by 18 publications

Purity & Quality Control

Choose Selective TGF-beta/Smad Inhibitors

Other TGF-beta/Smad Products

Biological Activity

Description K02288 is a potent, and selective type I BMP receptor inhibitor with IC50 of 1.1, 1.8, 6.4 nM for ALK2, ALK1 and ALK6, showing weaker inhibition on other ALKs (3, 4, 5) and ActRIIA.
Features A chemical probe for BMP signaling in cells.
Targets
ALK2 [1]
(Cell-free assay)
ALK1 [1]
(Cell-free assay)
ALK6 [1]
(Cell-free assay)
ALK3 [1]
(Cell-free assay)
1.1 nM 1.8 nM 6.4 nM 34.4 nM
In vitro

K02288 dose-dependently inhibits BMP4- and BMP6- induced Smad1/5/8 phosphorylation in C2C12 cells without affecting TGF-β-BMP signaling. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
C2C12 NGrENmxHfW6ldHnvckBie3OjeR?= M3\6XFMxKG2rboO= M2LXV2lvcGmkaYTpc44hd2ZiQl3QNk1qdmS3Y3XkJGJOWCC{ZXPldJRweiC2eYDlJFEhSUyNMjDpckBud3W|ZTDDNmMyOiClZXzsd{Bi\nSncjCzNEBucW6|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NF0xNjJ|zszN M2XyRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUCxPVEyLz5{NUGwNVkyOTxxYU6=
C2C12 MYHGeY5kfGmxbjDhd5NigQ>? NEjnPJo{OCCvaX7z NGjJOGVKdmirYnn0bY9vKG:oIFLNVFQucW6mdXPl[EBDVVBicnXj[ZB1d3JiaX6gcY92e2ViQ{LDNVIh[2WubIOgZYZ1\XJiM{CgcYlveyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEmFNUC9NE4{PM7:TR?= Mny2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMEG5NVEoRjJ3MUCxPVEyRC:jPh?=
C2C12 M{jxUGZ2dmO2aX;uJIF{e2G7 Mlz6N|AhdWmwcx?= NHnqeFVKdmirYnn0bY9vKG:oIFLNVFYucW6mdXPl[EBDVVBicnXj[ZB1d3JidInw[UAyKEGOS{KgbY4hdW:3c3WgR|JEOTJiY3XscJMh[W[2ZYKgN|AhdWmwczDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVA:OC52Mt88US=> M4mwWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUCxPVEyLz5{NUGwNVkyOTxxYU6=
HEK293T NGDhW2pHfW6ldHnvckBie3OjeR?= NFroT2w{OCCvaX7z NVLob5ppUW6qaXLpeIlwdiCxZjDUS2Zj\XSjMT3pcoR2[2WmIGTHSoJmfGFidInw[UAyKEGOS{WgbY4hUEWNMkmzWEBk\WyuczDh[pRmeiB|MDDtbY5{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3ME2zMlTPxE1? MlX1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMEG5NVEoRjJ3MUCxPVEyRC:jPh?=
Assay
Methods Test Index PMID
Western blot p-Smad1/5/8 / Smad1 ; p-Smad2 / Smad2 23646137
Growth inhibition assay Cell viability 23646137
In vivo In Zebrafish Embryos, K02288 induces a dorsalized phenotype in a dose dependent manner without effect om intersomitic vessel (ISV) formation. [1]

Protocol (from reference)

Animal Research:

[1]

  • Animal Models: Zebrafish Embryos
  • Dosages: ~10 µM
  • Administration: --

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 352.38
Formula

C20H20N2O4

CAS No. 1431985-92-0
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC1=CC(=CC(=C1OC)OC)C2=C(N=CC(=C2)C3=CC(=CC=C3)O)N

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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