For research use only. Not for use in humans.

Catalog No.S2618 Synonyms: DM3189

70 publications

LDN-193189 Chemical Structure

Molecular Weight(MW): 406.48

LDN-193189 is a selective BMP signaling inhibitor, inhibits the transcriptional activity of the BMP type I receptors ALK2 and ALK3 with IC50 of 5 nM and 30 nM in C2C12 cells, respectively, exhibits 200-fold selectivity for BMP versus TGF-β.

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Selleck's LDN-193189 has been cited by 70 publications

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Biological Activity

Description LDN-193189 is a selective BMP signaling inhibitor, inhibits the transcriptional activity of the BMP type I receptors ALK2 and ALK3 with IC50 of 5 nM and 30 nM in C2C12 cells, respectively, exhibits 200-fold selectivity for BMP versus TGF-β.
ALK2 [1]
(C2C12 cells)
ALK3 [1]
(C2C12 cells)
5 nM 30 nM
In vitro

LDN193189 potently inhibits BMP4-mediated Smad1, Smad5 and Smad8 activation with IC50 of 5 nM, and efficiently inhibits transcriptional activity of the BMP type I receptors ALK2 and ALK3 with IC50 of 5 nM and 30 nM, respectively. Furthermore, LDN193189 also shows the inhibitory effect on the transcriptional activity induced by either constitutively active ALK2R206H or ALK2Q207D mutant proteins. [1] A recent study shows that LDN-193189 blocks the production of reactive oxygen species induced by oxidized LDL during atherogenesis in human aortic endothelial cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
C2C12 NU\VOHpkU2mwYYPlJGF{e2G7 MlfhbY5pcWKrdIOgeIhmKGurbnHz[UBi[3Srdnn0fUBw\iCDTFu0JIFv\CCDY4TSTWlCKHerdHigTWM2OMLidnHseYV{KG:oIEGwNUBidmRiMkGwJI5uNCC{ZYPw[YN1cX[nbIm= MmTLNlU{Pjh|MkK=
EOC216 NVTkNoVoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVnBb2c3OC5zLUGwJO69VQ>? M1P4flIuOTBiZB?= MX3EUXNQ NXzZToFPcW6mdXPlJINmdGxiZHXheIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NX3xVmZLOjV{Mke4PVM>
OVAC429 MoO3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NE\RNWkzNzVizszN MoKzOFghcA>? MlnFSG1UVw>? NVrLc4tW\GWlcnXhd4V{KHSqZTDw[ZJk\W62YXflJI9nKGOnbHzzJIlvKFNicHjhdy=> MljYNlUzOjd6OUO=
SKOV3 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWOyM|Uh|ryP NGS5eJg1QCCq NV7oVpZCTE2VTx?= MYLk[YNz\WG|ZYOgeIhmKHCncnPlcpRi\2Vib3[gZ4VtdHNiaX6gV{BxcGG| MViyOVIzPzh7Mx?=
OVCA429 MmXvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{naclIwPSEQvF2= NYCxZYZPPDhiaB?= MYfEUXNQ MXPk[YNz\WG|ZYOgeIhmKHCncnPlcpRi\2Vib3[gZ4VtdHNiaX6gV{BxcGG| M4f1OlI2OjJ5OEmz
EOC219 Mnu0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWKyM|Uh|ryP MoeyOFghcA>? NXnyNoVTTE2VTx?= M4nwTYRm[3KnYYPld{B1cGVicHXyZ4VvfGGpZTDv[kBk\WyuczDpckBUKHCqYYO= MkHJNlUzOjd6OUO=
A549 NIDWfYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2nyN|AvPS1zNjFOwG0> Ml\ZSG1UVw>? NHLweoZqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? NYftS|QyOjR5N{iwNVE>
BEAS-2B  MlfOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVT0[ot{OC53LUG2JO69VQ>? Mke1SG1UVw>? MmTzbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NETK[W4zPDd5OECxNS=>
hBMSCs M{jqV2Z2dmO2aX;uJGF{e2G7 M4jvdlAvOsLizszN MlvOO{Bl MU\hZo9tcXOqZYOgeIhmKHOrbHnibY5qdi2ycn;tc5Rm\CCDTGCgZYN1cX[rdIpCpJBienSueR?= NGLDU3ozPDB5NkG4Oy=>
PANC-1 NUPYfWk4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXNV3FCPS13MECgcm0> NV;mO3RyPDhiaB?= Mn;WbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NGDUdJIzOzl4OUeyPS=>
MIA PaCa-2 M3vIN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFzBZXo2NTVyMDDuUS=> MVy0PEBp MULpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MY[yN|k3QTd{OR?=
Bx-PC3 NEPOT3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIDBUo02NTVyMDDuUS=> MoP5OFghcA>? NEHod2JqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? MUeyN|k3QTd{OR?=
PC3  MmLUSpVv[3Srb36gRZN{[Xl? NX;0NXhnPTByIH7N MUOyJIg> MnvDdoVxemW|c3XzJIFkfGm4YYTpc44hd2ZiU33h[FEwPS96IHHu[EBRNVOvYXSzJIxmfmWucx?= NYD2co8xOjJ2NUK4PFM>
EOC NX3HTIpiTnWwY4Tpc44hSXO|YYm= NUXnRnFuOTBvMUCwNEBvVQ>? MkixO|IhcA>? MYLy[YR2[2W|IITo[UBxcG:|cHjvdplt[XSnZDDCUXAhWi2VbXHkNU82NzkEoB?= NGroc24zOjJ2OUSxOS=>
HaCaT  NXnHTG9TTnWwY4Tpc44hSXO|YYm= MnXDNE4xODFvMUCg{txO MnfhNkBp MnuybY5pcWKrdIOgRm1ROi2rbnT1Z4VlKHCqb4PwbI9zgWyjdHnvckBw\iCVbXHkNU82Nzhid3n0bEBidiCLQ{WwxsBw\iC-MD6wNFXDqM7:TR?= NVWwNVlTOjF5NEC5OlY>
HaCaT  MkfZSpVv[3Srb36gRZN{[Xl? MkOyNE4xODFvMUCg{txO MXWyJIg> NFnyeFVqdmirYnn0d{B1cGViYXLpcIl1gSCxZjDBUGszKHSxIIDoc5NxcG:{eXzheIUhT1OWLWPtZYQyyqC5aYToJIFvKEmFNUFCpI9nKDR3wrDuUS=> MnLsNlE4PDB7Nk[=
HaCaT  NUK1PZJ5TnWwY4Tpc44hSXO|YYm= NFH4PJoxNjByMT2xNEDPxE1? MonaNkBp MlLZbY5pcWKrdIOgeIhmKGGkaXzpeJkhd2ZiQVzLN{B1dyCyaH;zdIhwenmuYYTlJHNu[WRzwrD3bZRpKGGwIFnDOVDDqG:oIEGwNEBvVQ>? MkDHNlE4PDB7Nk[=
HaCaT  NFK2[nlHfW6ldHnvckBCe3OjeR?= M4LseFAvODBzLUGwJO69VQ>? MVmyJIg> MoDVbY5pcWKrdIOgRWxMPCCjbnSgRWxMPSC5aYToJGlEPTEEoI\hcJVmeyCxZjCwMlPDqM7:TTDhcoQhOC53wrFOwG0hemW|cHXjeIl3\Wy7 NFvX[5YzOTd2MEm2Oi=>

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
pSMAD1 / pSMAD5 / pSMAD8 / SMAD1 / SMAD5 / SMAD8 / ID1 / SMAD4 / PARP / Cleaved PARP; 

PubMed: 31098401     

In vitro molecular pharmacology of LDN-193189 and LDN-214117. Western blot analysis of time- and concentration-dependent effects on downstream signalling in response to a LDN-193189 and b LDN-214117 in DIPG cells. Increasing concentrations (0–10 µM) at 4 and 8 h are shown for SU-DIPG-VI, HSJD-DIPG-IV and HSJD-DIPG-007. GAPDH is the loading control.

Tbx18 / Hcn4; 

PubMed: 30906456     

Representative immunofluorescence microscopy images of epicardial progenitor cells from the four groups with staining for Hcn4 (red). The nuclei were counterstained with DAPI (blue) and the YFP expressed by the cells is apparent (scale bar, 50 µm). (Tbx18 was conjugated to YFP).

Growth inhibition assay
Cell viability; 

PubMed: 31098401     

Screening of ALK2 inhibitors in vitro. Concentration-response curves for eight ALK2 inhibitors tested against three ACVR1 mutant cell cultures (HSJD-DIPG-007 (R206H), SU-DIPG-IV (G328V), HSJD-DIPG-018 (R258G), purple) and two wild-type cultures (SU-DIPG-VI, QCTB-R059, grey). a Pyrazolo[1,5-a]pyrimidines—dorsomorphin, LDN-193189, DMH1, LDN-212854. b Pyridines—K02288, LDN-214117, LDN-213844, LDN-213819. Concentration of compound is plotted on a log scale (x-axis) against cell viability (y-axis). Mean plus standard deviation are plotted from at least n = 3 experiments.

In vivo In conditional caALK2-transgenic mice with Ad.Cre on on postnatal day 7 (P7), LDN-193189 (3 mg/kg i.p) leads to mild calcifications surrounding the left tibia and fibula first visible at P13, and prevents radiographic lesions at P15 without causing weight loss or growth retardation, spontaneous fractures, decreased bone density or behavioral abnormalities. [1] LDN193189 dorsalizes zebrafish embryos by inhibiting signaling pathways induced by bone morphogenetic protein (BMP)6 without effect on vascular development. [2] In PCa-118b tumor-bearing mice, LDN-193189 treatment attenuates tumor growth and reduces bone formation in the tumors. [3] In LDL receptor-deficient (LDLR-/-) mice, LDN-193189 potently inhibits development of atheroma. Moreover, LDN-193189 also exhibits the inhibitory effects on associated vascular inflammation, osteogenic activity, and calcification. [4]


Animal Research:[1]
- Collapse
  • Animal Models: Ad.Cre on P7 is injected into conditional caALK2–transgenic and wild-type mice.
  • Formulation: LDN193189 is dissolved in DMSO and then diluted in water.
  • Dosages: ≤3 mg/kg
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO Insoluble
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
Saline (suspension)
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 406.48


CAS No. 1062368-24-4
Storage powder
in solvent
Synonyms DM3189

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID