Catalog No.S2618 Synonyms: DM3189

LDN-193189 Chemical Structure

Molecular Weight(MW): 406.48

LDN-193189 is a selective BMP signaling inhibitor, inhibits the transcriptional activity of the BMP type I receptors ALK2 and ALK3 with IC50 of 5 nM and 30 nM in C2C12 cells, respectively, exhibits 200-fold selectivity for BMP versus TGF-β.

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Cited by 15 Publications

5 Customer Reviews

  • LDN induces signifiant cell death. OVCA429 cells were treated with vehicle (DMSO) or experimental drugs for 6 hr to capture different stages of cell death. OVCA429 cell viability was assessed in response to drug treatment for 6 hr by staining with AO (green) and EB (orange). For clarity the different fluorescent channels to detect AO, EB, and the over-lapping images are shown for 10 mM LDN treatment.

    Int J Cancer 2014 136(5):E455-69. LDN-193189 purchased from Selleck.

    The small molecule BMP inhibitor LDN-193189 can partially block the increased BMP signaling in Jab1flox/flox, Col2a1-Cre mutants in a dose-dependent manner. Primary chondrocytes were cultured in DMEM medium containing 10% FBS and indicated amounts of LDN-193189 for 24 hours before being subjected to immunoblotting.

    J Cell Sci, 2013, 126: 234-43. LDN-193189 purchased from Selleck.

  • The density of muscle fibers and myoseptum are partially rescued in morphants treated with LDN to decrease BMP signaling (D–F) .These were fixed at 24 hpf and labeled with an antibody against Pax7, which strongly labels neural crest derived cells and also labels dermomyotome cells.

    Dev Biol 2014 378:107-121. LDN-193189 purchased from Selleck.

    (C) Smad1/5/9 phosphorylation and Smad1 protein levels were monitored in BMP-2 stimulated cells for up to 60 min. β-actin served as a loading control; BMP, bone morphogenetic protein.

    Oncol Rep, 2017, 37(2):713-720. LDN-193189 purchased from Selleck.

  • The influence of SB431542 and LDN193189 on a-SMA in A549 cells. (a) Representative images of immunofluorescence analysis for a-SMA using confocal microscopy are shown (scale bar ¼ 25 lm). (b) Relative densitometry diagram and typical western blot bands of a-SMA expression in the six groups (A–F). Data are expressed as mean ± SD (n ¼ 5 in each group). #p < 0.05 versus CONTROL group, p < 0.05 versus SILICA group

    Toxicol Mech Methods, 2018, 28(4):286-292. LDN-193189 purchased from Selleck.

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Biological Activity

Description LDN-193189 is a selective BMP signaling inhibitor, inhibits the transcriptional activity of the BMP type I receptors ALK2 and ALK3 with IC50 of 5 nM and 30 nM in C2C12 cells, respectively, exhibits 200-fold selectivity for BMP versus TGF-β.
ALK2 [1]
(C2C12 cells)
ALK3 [1]
(C2C12 cells)
5 nM 30 nM
In vitro

LDN193189 potently inhibits BMP4-mediated Smad1, Smad5 and Smad8 activation with IC50 of 5 nM, and efficiently inhibits transcriptional activity of the BMP type I receptors ALK2 and ALK3 with IC50 of 5 nM and 30 nM, respectively. Furthermore, LDN193189 also shows the inhibitory effect on the transcriptional activity induced by either constitutively active ALK2R206H or ALK2Q207D mutant proteins. [1] A recent study shows that LDN-193189 blocks the production of reactive oxygen species induced by oxidized LDL during atherogenesis in human aortic endothelial cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
C2C12 NGX5cohMcW6jc3WgRZN{[Xl? NInpSnJqdmirYnn0d{B1cGVia3nuZZNmKGGldHn2bZR6KG:oIFHMT|Qh[W6mIFHjeHJKUUFid3n0bEBKSzVywrD2ZYx2\XNib3[gNVAyKGGwZDCyNVAhdm1uIILld5Bm[3SrdnXsfS=> M3\6elI2OzZ6M{Ky
EOC216 M3LOdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWDCUIlpOC5zLUGwJO69VQ>? NYPBeodROi1zMDDk NHfiWplFVVOR M1Lt[Ilv\HWlZTDj[YxtKGSnYYToJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NVXPc5ZHOjV{Mke4PVM>
OVAC429 M4roWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVK1V3c{Oi93IN88US=> NXPkO3pXPDhiaB?= MUXEUXNQ M{m0RoRm[3KnYYPld{B1cGVicHXyZ4VvfGGpZTDv[kBk\WyuczDpckBUKHCqYYO= NHTEU4szPTJ{N{i5Ny=>
SKOV3 MoLoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVP1fHdLOi93IN88US=> Mk\OOFghcA>? MnjVSG1UVw>? NIXmUYFl\WO{ZXHz[ZMhfGinIIDldoNmdnSjZ3Wgc4Yh[2WubIOgbY4hWyCyaHHz NYXYR2xTOjV{Mke4PVM>
OVCA429 M1;HOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVL0fnFqOi93IN88US=> M3jsU|Q5KGh? MnzCSG1UVw>? Mmjp[IVkemWjc3XzJJRp\SCyZYLj[Y51[WenIH;mJINmdGy|IHnuJHMheGijcx?= MXKyOVIzPzh7Mx?=
EOC219 NE\ZbXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYmyM|Uh|ryP MVu0PEBp NGPUbnFFVVOR NEW5XVVl\WO{ZXHz[ZMhfGinIIDldoNmdnSjZ3Wgc4Yh[2WubIOgbY4hWyCyaHHz NYKxfnV1OjV{Mke4PVM>
A549 NUOxNYh4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlnONE42NTF4IN88US=> NIC3TWlFVVOR NYTQS3RQcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M1TFT|I1Pzd6MEGx
BEAS-2B  NUnGSIFOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWGwMlUuOTZizszN NVn3S5JsTE2VTx?= NXrhVJdrcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? Mn\jNlQ4PzhyMUG=
hBMSCs M2mzVWZ2dmO2aX;uJGF{e2G7 M2TJOlAvOsLizszN NH7wWnI4KGR? M3mwU4Fjd2yrc3jld{B1cGVic3nsbYJqdmmwLYDyc41wfGWmIFHMVEBi[3Srdnn0feKheGG{dHz5 MkfXNlQxPzZzOEe=
PANC-1 NEfS[FBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXS1MVUxOCCwTR?= M3HJTlQ5KGh? MmTYbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NH\yeoIzOzl4OUeyPS=>
MIA PaCa-2 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1zsXVUuPTByIH7N MXG0PEBp MWXpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MYeyN|k3QTd{OR?=
Bx-PC3 MornS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYK1MVUxOCCwTR?= NUD1RmtFPDhiaB?= Mly4bY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> M2rrVVI{QTZ7N{K5
PC3  MVvGeY5kfGmxbjDBd5NigQ>? NXnicJRGPTByIH7N NHG3UVMzKGh? MlfVdoVxemW|c3XzJIFkfGm4YYTpc44hd2ZiU33h[FEwPS96IHHu[EBRNVOvYXSzJIxmfmWucx?= NXy2Uo9iOjJ2NUK4PFM>
LNCaP MlHwSpVv[3Srb36gRZN{[Xl? MUGw5qCUPTByIH7N NInmfYozKGh? MWjy[ZZmenOnczDyZZBidXmlaX6tbY5lfWOnZDDj[YxtKGSnYYTo M1\UNVIzPDV{OEiz
HaCaT  M2\YSGZ2dmO2aX;uJGF{e2G7 M3ztRVAvODBzLUGwJO69VQ>? NGnXW4YzKGh? NFjBWppqdmirYnn0d{BDVVB{LXnu[JVk\WRicHjvd5Bpd3K7bHH0bY9vKG:oIGPtZYQyNzVxODD3bZRpKGGwIFnDOVDDqG:oII6wMlAxPcLizszN NIHZNWczOTd2MEm2Oi=>
HaCaT  MV\GeY5kfGmxbjDBd5NigQ>? MVOwMlAxOS1zMDFOwG0> NX\4e5NLOiCq NX3R[HJNcW6qaXLpeJMhfGinIHHibYxqfHlib3[gRWxMOiC2bzDwbI9{eGixconsZZRmKEeVVD3TcYFlOcLid3n0bEBidiCLQ{WwxsBw\iB2NdMgcm0> MV[yNVc1ODl4Nh?=
HaCaT  NHKxS5hHfW6ldHnvckBCe3OjeR?= M3e3WFAvODBzLUGwJO69VQ>? MVuyJIg> NHvoWFNqdmirYnn0d{B1cGViYXLpcIl1gSCxZjDBUGs{KHSxIIDoc5NxcG:{eXzheIUhW22jZEJCpJdqfGhiYX6gTWM2OMLib3[gNVAxKG6P M1\u[VIyPzRyOU[2
HaCaT  M4LSTWZ2dmO2aX;uJGF{e2G7 MXWwMlAxOS1zMDFOwG0> MnHmNkBp M2HOb4lvcGmkaYTzJGFNUzRiYX7kJGFNUzVid3n0bEBKSzVywrD2ZYx2\XNib3[gNE4{yqEQvF2gZY5lKDBwNdMg{txOKHKnc4DlZ5RqfmWueR?= NGTGWWwzOTd2MEm2Oi=>

... Click to View More Cell Line Experimental Data

In vivo In conditional caALK2-transgenic mice with Ad.Cre on on postnatal day 7 (P7), LDN-193189 (3 mg/kg i.p) leads to mild calcifications surrounding the left tibia and fibula first visible at P13, and prevents radiographic lesions at P15 without causing weight loss or growth retardation, spontaneous fractures, decreased bone density or behavioral abnormalities. [1] LDN193189 dorsalizes zebrafish embryos by inhibiting signaling pathways induced by bone morphogenetic protein (BMP)6 without effect on vascular development. [2] In PCa-118b tumor-bearing mice, LDN-193189 treatment attenuates tumor growth and reduces bone formation in the tumors. [3] In LDL receptor-deficient (LDLR-/-) mice, LDN-193189 potently inhibits development of atheroma. Moreover, LDN-193189 also exhibits the inhibitory effects on associated vascular inflammation, osteogenic activity, and calcification. [4]


Animal Research:[1]
+ Expand
  • Animal Models: Ad.Cre on P7 is injected into conditional caALK2–transgenic and wild-type mice.
  • Formulation: LDN193189 is dissolved in DMSO and then diluted in water.
  • Dosages: ≤3 mg/kg
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO Insoluble
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
Saline (suspension)
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 406.48


CAS No. 1062368-24-4
Storage powder
in solvent
Synonyms DM3189

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID