Home TGF-beta/Smad TGF-beta/Smad inhibitor LDN-193189

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LDN-193189 BMP Signaling inhibitor

Cat.No.S2618

LDN-193189 (DM3189) is a selective BMP signaling inhibitor, inhibits the ALK1, ALK2, ALK3 and ALK6 with IC50s of 0.8 nM, 0.8 nM, 5.3 nM and 16.7 nM in the kinase assay, respectively. LDN-193189 inhibits the transcriptional activity of the BMP type I receptors ALK2 and ALK3 with IC50s of 5 nM and 30 nM in C2C12 cells, respectively, exhibits 200-fold selectivity for BMP versus TGF-β. For cell testing, the water-soluble S7507 LDN-193189 2HCl is recommended.This product has poor solubility, animal experiments are available, cell experiments please choose carefully!
LDN-193189 TGF-beta/Smad inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 406.48
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Quality Control

Batch: Purity: 99.43%
99.43

Solubility in DMSO or Water

In vitro
Batch:

0.01M HCl : 2.5 mg/mL

DMSO : Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

The solubility data above are all experimental results (not literature values).

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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Working Concentrations for Cell Culture Treatment

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Working Concentrations for Animal Model Treatment

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Mechanism of Action

Information LDN-193189 is a potent, ATP-competitive BMP receptor (ALK1/2/3) inhibitor. It affects the BMP/Smad signaling pathway by blocking Smad1/5/8 phosphorylation, effectively inhibiting tumor progression and osteoblast differentiation while promoting stem cell neural differentiation.

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Primary Applications and Mechanism of Action

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Chemical Information, Storage & Stability

Molecular Weight 406.48 Formula

C25H22N6

 Storage (From the date of receipt)
CAS No. 1062368-24-4 Download SDF Storage of Stock Solutions

Synonyms DM3189 Smiles C1CN(CCN1)C2=CC=C(C=C2)C3=CN4C(=C(C=N4)C5=CC=NC6=CC=CC=C56)N=C3

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