ML347

Catalog No.S7148 Synonyms: LDN-193719

For research use only.

ML347 (LDN-193719) is a selective BMP receptor inhibitor with IC50 of 32 nM for ALK2, >300-fold selectivity over ALK3. Also inhibits ALK1 activity with IC50 of 46 nM.

ML347 Chemical Structure

CAS No. 1062368-49-3

Selleck's ML347 has been cited by 4 Publications

Purity & Quality Control

Choose Selective TGF-beta/Smad Inhibitors

Other TGF-beta/Smad Products

Biological Activity

Description ML347 (LDN-193719) is a selective BMP receptor inhibitor with IC50 of 32 nM for ALK2, >300-fold selectivity over ALK3. Also inhibits ALK1 activity with IC50 of 46 nM.
Targets
ALK2 [1]
(Cell-free assay)
ALK1 [1]
(Cell-free assay)
32 nM 46 nM
In vitro

ML347 also shows potency in BMP-responsive C2C12BRA cells with IC50 of 15 nM, and designated an MLPCN probe. ML347 displays high plasma protein binding in human, rat and mouse plasma. [1]

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 10 mg/mL
(28.37 mM)
Water Insoluble
Ethanol Insoluble

Chemical Information

Molecular Weight 352.39
Formula

C22 H16 N4

CAS No. 1062368-49-3
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC1=CC=C(C=C1)C2=CN3C(=C(C=N3)C4=C5C=CC=NC5=CC=C4)N=C2

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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