Name: Ramipril
Brand: Selleck Chemicals
SKU: S1793
Availability: InStock
Rating: 5 (2 reviews)

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Quality Control

Batch: Purity: 99.46%

99.46

Related Targets	Adrenergic Receptor Estrogen/progestogen Receptor GPR Androgen Receptor Glucocorticoid Receptor ACE Progesterone Receptor Opioid Receptor THR PGES
Other RAAS Inhibitors	Temocapril HCl VTP-27999 TFA Imidapril HCl Delapril Hydrochloride Zofenopril calcium Temocapril

Solubility

In vitro
Batch:

DMSO : 83 mg/mL (199.27 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 83 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.150mg/ml (9.96mM)	Taking the 1 mL working solution as an example, add 50 μL 83 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	416.51	Formula	C₂₃H₃₂N₂O₅	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	87333-19-5	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	HOE-498			SMILES	CCOC(=O)C(CCC1=CC=CC=C1)NC(C)C(=O)N2C3CCCC3CC2C(=O)O

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

Features	A pro-drug converted to its active metabolite, ramiprilat, by liver esterase enzymes.
Targets/IC₅₀/Ki	ACE 5 nM
In vitro	Ramipril is an angiotensin-converting enzyme (ACE) inhibitor with IC50 of 5 nM. This compound enhances the activity of ACE-associated CK2 and the phosphorylation of ACE Ser¹²⁷⁰ in cultured endothelial cells, but is unable to activate JNK or stimulate the nuclear accumulation of c-Jun in endothelial cells expressing a S1270A ACE mutant or in ACE-deficient cells. Prolonged treatment with this chemical increases ACE expression in primary cultures of human endothelial cells and in vivo (mouse lung), which can be prevented by pretreatment with the JNK inhibitor SP600125. It displays little enhanced effect on the rate of in vitro endothelial apoptosis induced by the serum deprivation method.
In vivo	Chronic in vivo administration of Ramipril to rats at a dosage that has similar hypotensive effects in vitro HUVECs significantly reduces the rate of LPS-induced apoptosis compared to the other ACE inhibitors, which contrasts with the apoptosis effect in vitro. This compound inhibits systolic blood pressure (SBP) with IC50 of 1.97 mg/kg in spontaneously hypertensive rats (SHR). When in combination with AT1-receptor blockade by candesartan-cilexetil increases SBP reduction synergistically rather than additively. Administration of this chemical to spontaneously hypertensive rats (SHR) produces significant inhibition of aorta ACE and lung ACE with IC50 ~5 mg/kg, but shows little effect for brain ACE ex vivo. This agent prevents beta cell dysfunction in osteoprotegerin treated mice through decreasing islet monocyte/macrophage infiltration, fibrosis and apoptosis involving decreasing RAS, growth factor genes and inflammatory molecules expressions.
References	[1] https://pubmed.ncbi.nlm.nih.gov/2905962/ [2] https://pubmed.ncbi.nlm.nih.gov/14615289/ [3] https://pubmed.ncbi.nlm.nih.gov/18004652/ [4] https://pubmed.ncbi.nlm.nih.gov/15076168/ [5] https://pubmed.ncbi.nlm.nih.gov/2557876/ [6] https://pubmed.ncbi.nlm.nih.gov/20832449/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-07-31)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05508035	RECRUITING	Heart Failure With Moderately Reduced Ejection Fraction	John Paul II Hospital, Krakow	2023-07-13	PHASE3
NCT06562582	RECRUITING	Myocardial Infarct	Vastra Gotaland Region	2025-01-06	PHASE4
NCT03770936	RECRUITING	Liver Fibroses	Sherief Abd-Elsalam	2018-10-01	PHASE3
NCT02842424	RECRUITING	Peripheral Arterial Disease	University of Nebraska	2016-02-25	PHASE4
NCT05030818	RECRUITING	Coronary Artery Disease	Fundación EPIC	2022-10-14	PHASE4
NCT07259512	RECRUITING	Chronic Kidney Disease; Heart Failure With Reduced Ejection Fraction (HFrEF)	Evi Liliek Wulandari	2025-06-30	PHASE4

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Ramipril RAAS inhibitor

Chemical Structure

Molecular Weight: 416.51