Berberine chloride (NSC 646666)

Catalog No.S2271 Synonyms: Natural Yellow 18 chloride

For research use only.

Berberine chloride (NSC 646666, Natural Yellow 18) is a quaternary ammonium salt from the group of isoquinoline alkaloids. Berberine activates caspase 3 and caspase 8, cleavage of poly ADP-ribose polymerase (PARP) and the release of cytochrome c. Berberine chloride decreases the expression of c-IAP1, Bcl-2 and Bcl-XL. Berberine chloride induces apoptosis with sustained phosphorylation of JNK and p38 MAPK, as well as generation of the ROS. Berberine chloride is a dual topoisomerase I and II inhibitor. Berberine chloride is also a potential autophagy modulator.

Berberine chloride (NSC 646666) Chemical Structure

CAS No. 633-65-8

Selleck's Berberine chloride (NSC 646666) has been cited by 6 Publications

Purity & Quality Control

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Biological Activity

Description Berberine chloride (NSC 646666, Natural Yellow 18) is a quaternary ammonium salt from the group of isoquinoline alkaloids. Berberine activates caspase 3 and caspase 8, cleavage of poly ADP-ribose polymerase (PARP) and the release of cytochrome c. Berberine chloride decreases the expression of c-IAP1, Bcl-2 and Bcl-XL. Berberine chloride induces apoptosis with sustained phosphorylation of JNK and p38 MAPK, as well as generation of the ROS. Berberine chloride is a dual topoisomerase I and II inhibitor. Berberine chloride is also a potential autophagy modulator.
Targets
Caspase-3 [1]
()
Caspase-8 [1]
()
PARP [1]
()
cytochrome c [1]
()
cIAP1 [1]
()
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Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MRC5 cells MU\GeY5kfGmxbjDhd5NigQ>? M3jONmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhEVVZiaX6gUXJEPSClZXzsd{BjgSCybHHxeYUhemWmdXP0bY9vKGG|c3H5MEBKSzVyPUCuOlgh|ryP NHrGSGkyPzJ|OUW5OC=>
MRC5 cells MmLnVJJwdGmoZYLheIlwdiCjc4PhfS=> MYWxNEDPxE1? NWXGUGJ6PTRiaILz NH\LWpVKdmirYnn0bY9vKG:oIFjDUXYheHKxbHnm[ZJifGmxbjDpckBOWkN3IHPlcIx{KGGodHXyJFU1KGi{czDwc5N1NWmwZnXjeIlwdiCjdDCxNEB2VSCkeTDwcIFyfWViYYPzZZk> M4rr[FE4OjN7NUm0
MRC5 cells NH31[5hRem:uaX\ldoF1cW:wIHHzd4F6 MYixNEDPxE1? NInZXVUzPCCq M1Gxb2lvcGmkaYTpc44hd2ZiSFPNWkBxem:uaX\ldoF1cW:wIHnuJG1TSzViY3XscJMh[W[2ZYKgNlQhcHK|IIDvd5QucW6oZXP0bY9vKGG2IEGwJJVOKGK7IIDsZZF2\SCjc4PhfS=> MXSxO|I{QTV7NB?=
Bel7402 cells Mo\TSpVv[3Srb36gZZN{[Xl? MYmxNkBp MVvJcoR2[3Srb36gc4YhVESOUjDwdo91\WmwIHnuJIh2dWGwIFLlcFc1ODJiY3XscJMh[W[2ZYKgNVIhcHK|IHL5JHJVNVCFUjDhd5NigSC{ZXzheIl3\SC2bzDjc451em:u M1z3V|E6ODlyN{[3
HepG2 cells M1zZemZ2dmO2aX;uJIF{e2G7 M{TFUlExKHWpL33M NXnVdVJsOTJiaB?= M4P0XGlv\HWldHnvckBw\iCORFzSJJBzd3SnaX6g[ZhxemW|c3nvckBqdiCqdX3hckBJ\XCJMjDj[YxteyCjdDCxNEB2\y:vTDDh[pRmeiBzMjDodpMh[nliZnzve{BkgXSxbXX0dpk> MUexPVA6ODd4Nx?=
KB cells M4D1eGN6fG:2b4jpZ4l1gSCjc4PhfS=> M3TiVFczKGh? M{PEdWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGtDKGOnbHzzJIFnfGW{IEeyJIhzeyxiSVO1NF04NjN{IN88US=> NEjDZncyOTF2MUGwOS=>
HL60 cells NUS5fmk3SXCxcITvd4l{KGG|c3H5 M{X4c|Q5KGi{cx?= M2DxZ2lv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iSFy2NEBk\WyuczDh[pRmeiB2ODDodpMhfXOrbnegZY5v\XirbjDWMZBzd3CrZHn1cUBqd2SrZHWgd5RicW6rbnegZpkhTkGFUzDhcoFtgXOrcx?= MYWyNFIzPzF2NB?=
A549 cells NIizfpdEgXSxdH;4bYNqfHliYYPzZZk> NX[0fXNQS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCkeTDTVmIh[XO|YYmsJGlEPTB;Nj6yO{DPxE1? M{\3eFIxPTl2OES4
SKOV3 cells MkO1R5l1d3SxeHnjbZR6KGG|c3H5 NYDnXJI1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0uRVkOgZ4VtdHNiYomgV3JDKGG|c3H5MEBKSzVyPUG2MlQ1KM7:TR?= M{jtSlIxPTl2OES4
SK-MEL-2 cells MnrZR5l1d3SxeHnjbZR6KGG|c3H5 NHjQR45EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUUy2PRVytNkBk\WyuczDifUBUWkJiYYPzZZktKEmFNUC9NVMvPzZizszN MVeyNFU6PDh2OB?=
HCT15 cells M2fMUGN6fG:2b4jpZ4l1gSCjc4PhfS=> M4rURWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDF3IHPlcIx{KGK7IGPSRkBie3OjeTygTWM2OD1zNj61PUDPxE1? M{Syd|IxPTl2OES4
CEM cells NHG2TGxEgXSxdH;4bYNqfHliYYPzZZk> NFzNdW41QCCqcoO= M1TwSmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGNGVSClZXzsd{BmgHC{ZYPzbY5oKGe{ZXXuJIZtfW:{ZYPj[Y51KHC{b4TlbY4h[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhS0N3ME2yMlA6KM7:TR?= MVeyNVI6PTh7MR?=
human CEM cells MlT2SpVv[3Srb36gZZN{[Xl? Mo\KO{Bl[Xm| NWDlTVlvSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhOC5yNTDNU2khUHWvYX6gbY1ufW6xZHXmbYNq\W6leTD2bZJ2eyBzIF7MOE4{KGmwZnXjeIVlKGmwIHj1cYFvKEOHTTDj[YxteyCneIDy[ZN{cW6pIHfy[YVvKG[udX;y[ZNk\W62IIDyc5RmcW5iYYPz[ZN{\WRiYYOgdFI1KGGwdHnn[Y4heHKxZIXjeIlwdiCvZXHzeZJm\CB5IHThfZMheG:|dDDpcoZm[3Srb36gZpkhTUyLU1GsJGVEPTB;MD6xN{DPxE1? NXzqc2FwOjF{OUW4PVE>
SKN cells MnTPS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHi0[I44OiCq MYLHdo94fGhiaX7obYJqfGmxbjDh[4FqdnO2IHj1cYFvKFONTjDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCJSUWwQVE2Njh6IN88US=> MoPhNlE1ODFzMUS=
RKN cells NXfFVW1WT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHj0fnI1QCCqcoO= NVuwOY9TT3Kxd4ToJIlvcGmkaYTpc44h[WejaX7zeEBpfW2jbjDST24h[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygS2k2OD12OT62JO69VQ>? M4fhS|IyPDBzMUG0
G402 cells NETCTmFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGGwfXY1QCCqcoO= MVzHdo94fGhiaX7obYJqfGmxbjDh[4FqdnO2IHj1cYFvKEd2MEKgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiR1m1NF0yOS56NzFOwG0> NGXvVmwzOTRyMUGxOC=>
A10 cells MUnGeY5kfGmxbjDhd5NigQ>? M4nPOFMxKM7:TR?= M4PwNFI1KGi{cx?= MnzDSI94dnKnZ4XsZZRqd25ib3[gV4NlOiCvUl7BJIV5eHKnc4Ppc44hcW5icnH0JGEyOCClZXzsd{BifCB|MDD1UUBi\nSncjCyOEBpenNiYomgdZVidnSrdHH0bZZmKFKWLWDDVkBidmGueYPpdy=> NGLqPJIzOTRyMUGxOC=>
A10 cells NXzEepRSTnWwY4Tpc44h[XO|YYm= M173NlMxKM7:TR?= MYiyOEBpenN? MlPRSI94diC{ZXf1cIF1cW:wIH;mJHBzcW1{IH3SUmEh\XiycnXzd4lwdiCrbjDyZZQhSTFyIHPlcIx{KGG2IEOwJJVOKGGodHXyJFI1KGi{czDifUByfWGwdHn0ZZRqfmViUmStVGNTKGGwYXz5d4l{ M2[4OVIyPDBzMUG0
A10 cells M3PYUWZ2dmO2aX;uJIF{e2G7 NUj5[nBEOzBizszN NGrERmwzPCCqcoO= MUDEc5dvemWpdXzheIlwdiCxZjDJcZBsKG2UTlGg[ZhxemW|c3nvckBqdiC{YYSgRVExKGOnbHzzJIF1KDNyIIXNJIFnfGW{IEK0JIhzeyCkeTDxeYFvfGm2YYTpeoUhWlRvUFPSJIFv[Wy7c3nz MnXtNlE1ODFzMUS=
HepG2-A16-CD81 cells NGj3UotHfW6ldHnvckBie3OjeR?= NXTVfHZDOTBizszN M3TWWG5QXkGUVFnTPkBCdnSrbXHsZZJq[WxibHn2[ZIhe3SjZ3WgZYN1cX[rdImgcYVie3W{ZXSgZZMh[SCpcnXheIVzKHSqYX6gOVAmKHKnZIXjeIlwdiCrbjDQcIF{dW:maYXtJJlw\WyraTDzZ4hqgm:wdDDhdoViKGmwIFjldGczNUFzNj3DSFgyKGOnbHzzJIF1KDFydV2gZ49ueG:3bnSgZ49v[2WwdILheIlwdixiZHX0[ZJucW6nZDDifUBqdW23bn:t[ox2d3Knc3PlcoNmNg>? NGr4eGczOjB7NkGwNS=>
HepG2-A16-CD81 cells NWLBUmVGTnWwY4Tpc44h[XO|YYm= NISxT4UyOCEQvF2= MnniUm9XSVKWSWO6JGFvfGmvYXzhdolidCCuaY\ldkB{fGGpZTDhZ5Rqfmm2eTDt[YF{fXKnZDDhd{Bz\WS3Y4Tpc44hcW5iUHzhd41w\Gm3bTD5c4VtcWlic3PobZpwdnRiYYLlZUBqdiCKZYDHNk1COTZvQ1S4NUBk\WyuczDifUBqdW23bn:t[ox2d3Knc3PlcoNmNCCjbnSgcYVlcWGwIIPjbIl7d262IIPpfoUh[XRiMUD1UUBkd22yb4Xu[EBkd26lZX70doF1cW:wLDDJR|UxRTBwNUS4JO69VQ>? NGHMNoozOjB7NkGwNS=>
HepG2 cells M2fyTmZ2dmO2aX;uJIF{e2G7 NYeySpVXOTBizszN Ml7ZOEBp MmTaTY5kemWjc3WgbY4hSU2SS3HsdIhiKHCqb4PwbI9zgWyjdHnvckBqdiCqdX3hckBJ\XCJMjDj[YxteyCjdDCxNEB2VSCjZoTldkA1KGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXNicnXsZZRqfmVidH:geY51emWjdHXkJINwdnS{b3y= MkLONlMxPThzMEe=
HepG2 cells Ml\1SpVv[3Srb36gZZN{[Xl? NIfoO3gyOCEQvF2= NVLrPJdFPCCq NVvDWXFsUW6lcnXhd4UhcW5idH;0ZYwhSU2SS3HsdIhiKGyndnXsJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGG2IEGwJJVOKGGodHXyJFQhcHK|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqeyC{ZXzheIl3\SC2bzD1cpRz\WG2ZXSgZ49vfHKxbB?= NXPQeVEyOjNyNUixNFc>
HepG2 cells M2\kfmZ2dmO2aX;uJIF{e2G7 M3zwSlIxKM7:TR?= NXLBOXE4OjRiaILz MVnJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKEincFeyJINmdGy|IHHzd4V{e2WmIHHzJI1wenCqb3zv[4lk[WxiY3jhcodmeyCjdDCyNEB2VSCjZoTldkAzPCCqcoOgeZNqdmdiSH;lZ4h{fCB|M{K1PEB{fGGrbnnu[{BjgSCobIXvdoV{[2WwY3WgcYlkem:|Y3;wbYMh[W6jbInzbZM> MVOyN|E5OjB6OB?=
HT-29 cells NXG0dpN6S3m2b4TvfIlkcXS7IHHzd4F6 NYntcHVnPDhiaILz NYLONFFlS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUFRvMkmgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF05NjR3IN88US=> MVqyN|E5OjB6OB?=
HepG2 cells NGH2SWlEgXSxdH;4bYNqfHliYYPzZZk> MnzBNlQhcHK| MVnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjCyOEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUGxMlIzKM7:TR?= NWj4V2pPOjNzOEKwPFg>
HepG2 cells MXrDfZRwfG:6aXPpeJkh[XO|YYm= NYHWcWZlPDhiaILz MmjXR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME24MlMzKM7:TR?= Mn;DNlMyQDJyOEi=

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 40 mg/mL
(107.58 mM)
Water Insoluble
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.

3mg/mL

Chemical Information

Molecular Weight 371.81
Formula

C20H18NO4.Cl

CAS No. 633-65-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC1=C(C2=C[N+]3=C(C=C2C=C1)C4=CC5=C(C=C4CC3)OCO5)OC.[Cl-]

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03281096 Unknown status Drug: Berberine hydrochloride|Drug: Placebo Colorectal Adenomas xiaohua li|Xijing Hospital of Digestive Diseases August 29 2017 Phase 2|Phase 3
NCT02806999 Unknown status Drug: Berberine; Insulin|Drug: Insulin Hyperglycemia Guangdong Provincial People''s Hospital July 2016 Phase 4

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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