Berberine chloride (NSC 646666)

For research use only.

Catalog No.S2271 Synonyms: Natural Yellow 18 chloride

5 publications

Berberine chloride (NSC 646666) Chemical Structure

CAS No. 633-65-8

Berberine chloride (NSC 646666, Natural Yellow 18) is a quaternary ammonium salt from the group of isoquinoline alkaloids. Berberine activates caspase 3 and caspase 8, cleavage of poly ADP-ribose polymerase (PARP) and the release of cytochrome c. Berberine chloride decreases the expression of c-IAP1, Bcl-2 and Bcl-XL. Berberine chloride induces apoptosis with sustained phosphorylation of JNK and p38 MAPK, as well as generation of the ROS. Berberine chloride is a dual topoisomerase I and II inhibitor. Berberine chloride is also a potential autophagy modulator.

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10mM (1mL in DMSO) USD 90 In stock
USD 70 In stock
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Selleck's Berberine chloride (NSC 646666) has been cited by 5 publications

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Biological Activity

Description Berberine chloride (NSC 646666, Natural Yellow 18) is a quaternary ammonium salt from the group of isoquinoline alkaloids. Berberine activates caspase 3 and caspase 8, cleavage of poly ADP-ribose polymerase (PARP) and the release of cytochrome c. Berberine chloride decreases the expression of c-IAP1, Bcl-2 and Bcl-XL. Berberine chloride induces apoptosis with sustained phosphorylation of JNK and p38 MAPK, as well as generation of the ROS. Berberine chloride is a dual topoisomerase I and II inhibitor. Berberine chloride is also a potential autophagy modulator.
Targets
Caspase-3 [1]
()
Caspase-8 [1]
()
PARP [1]
()
cytochrome c [1]
()
cIAP1 [1]
()
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MRC5 cells NV\IWXMzTnWwY4Tpc44h[XO|YYm= MUDBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDIR21XKGmwIF3SR|Uh[2WubIOgZpkheGyjcYXlJJJm\HWldHnvckBie3OjeTygTWM2OD1yLk[4JO69VQ>? MYexO|I{QTV7NB?=
MRC5 cells M1vUb3Bzd2yrZnXyZZRqd25iYYPzZZk> MX:xNEDPxE1? MWC1OEBpenN? NWf1UVNGUW6qaXLpeIlwdiCxZjDIR21XKHC{b3zp[oVz[XSrb36gbY4hVVKFNTDj[YxteyCjZoTldkA2PCCqcoOgdI9{fC2rbn\lZ5Rqd25iYYSgNVAhfU1iYomgdIxieXWnIHHzd4F6 NW\2boNrOTd{M{m1PVQ>
MRC5 cells MWfQdo9tcW[ncnH0bY9vKGG|c3H5 MXuxNEDPxE1? NXjrV|NHOjRiaB?= M2\WZ2lvcGmkaYTpc44hd2ZiSFPNWkBxem:uaX\ldoF1cW:wIHnuJG1TSzViY3XscJMh[W[2ZYKgNlQhcHK|IIDvd5QucW6oZXP0bY9vKGG2IEGwJJVOKGK7IIDsZZF2\SCjc4PhfS=> MXmxO|I{QTV7NB?=
Bel7402 cells MnfZSpVv[3Srb36gZZN{[Xl? Mlz2NVIhcA>? M2DZVWlv\HWldHnvckBw\iCORFzSJJBzd3SnaX6gbY4hcHWvYX6gRoVtPzRyMjDj[YxteyCjZoTldkAyOiCqcoOgZpkhWlRvUFPSJIF{e2G7IILlcIF1cX[nIITvJINwdnS{b3y= NIDPO3IyQTB7MEe2Oy=>
HepG2 cells M{TQemZ2dmO2aX;uJIF{e2G7 Mk\nNVAhfWdxbVy= M2XyNFEzKGh? NUjjWmg{UW6mdXP0bY9vKG:oIFzEUHIheHKxdHXpckBmgHC{ZYPzbY9vKGmwIHj1cYFvKEincFeyJINmdGy|IHH0JFExKHWpL33MJIFnfGW{IEGyJIhzeyCkeTDmcI94KGO7dH;t[ZRzgQ>? MYWxPVA6ODd4Nx?=
KB cells M2fEbGN6fG:2b4jpZ4l1gSCjc4PhfS=> MoPYO|IhcA>? MknxR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2Ih[2WubIOgZYZ1\XJiN{KgbJJ{NCCLQ{WwQVcvOzJizszN NG\MepUyOTF2MUGwOS=>
HL60 cells NY\Mb4V[SXCxcITvd4l{KGG|c3H5 MYq0PEBpenN? MnLKTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCKTE[wJINmdGy|IHHmeIVzKDR6IHjyd{B2e2mwZzDhco5mgGmwIG[tdJJweGmmaYXtJIlw\GmmZTDzeIFqdmmwZzDifUBHSUOVIHHuZYx6e2m| MXqyNFIzPzF2NB?=
A549 cells NF3pNoJEgXSxdH;4bYNqfHliYYPzZZk> NWO3VXc3S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCkeTDTVmIh[XO|YYmsJGlEPTB;Nj6yO{DPxE1? NGT6RZgzODV7NEi0PC=>
SKOV3 cells M1\lOWN6fG:2b4jpZ4l1gSCjc4PhfS=> M{PzcmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNMV1Z|IHPlcIx{KGK7IGPSRkBie3OjeTygTWM2OD1zNj60OEDPxE1? NGfNRXkzODV7NEi0PC=>
SK-MEL-2 cells M1vqTWN6fG:2b4jpZ4l1gSCjc4PhfS=> MmKxR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2suVUWOLUKgZ4VtdHNiYomgV3JDKGG|c3H5MEBKSzVyPUGzMlc3KM7:TR?= MXKyNFU6PDh2OB?=
HCT15 cells NIq4c3VEgXSxdH;4bYNqfHliYYPzZZk> MlzCR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVOTViY3XscJMh[nliU2LCJIF{e2G7LDDJR|UxRTF4LkW5JO69VQ>? M176bVIxPTl2OES4
CEM cells NFzHZ2REgXSxdH;4bYNqfHliYYPzZZk> MW[0PEBpenN? M3q5Z2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGNGVSClZXzsd{BmgHC{ZYPzbY5oKGe{ZXXuJIZtfW:{ZYPj[Y51KHC{b4TlbY4h[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhS0N3ME2yMlA6KM7:TR?= MkTqNlEzQTV6OUG=
human CEM cells MX;GeY5kfGmxbjDhd5NigQ>? M4PXeVch\GG7cx?= M4PiWmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JFAvODViTV;JJGh2dWGwIHntcZVvd2SnZnnjbYVv[3lidnnyeZMhOSCQTESuN{Bqdm[nY4Tl[EBqdiCqdX3hckBETU1iY3XscJMh\XiycnXzd4lv\yCpcnXlckBndHWxcnXzZ4VvfCCycn;0[YlvKGG|c3Xzd4VlKGG|IICyOEBidnSrZ3XuJJBzd2S3Y4Tpc44hdWWjc4Xy[YQhPyCmYYnzJJBwe3RiaX7m[YN1cW:wIHL5JGVNUVODLDDFR|UxRTBwMUOg{txO M2CxNVIyOjl3OEmx
SKN cells NFe3XotIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXi3NkBp MYPHdo94fGhiaX7obYJqfGmxbjDh[4FqdnO2IHj1cYFvKFONTjDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCJSUWwQVE2Njh6IN88US=> M3fJcVIyPDBzMUG0
RKN cells NEP6cWlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX[0PEBpenN? MljLS5Jwf3SqIHnubIljcXSrb36gZYdicW6|dDDoeY1idiCUS16gZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiR1m1NF01QS54IN88US=> MnjhNlE1ODFzMUS=
G402 cells MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXz6e3F[PDhiaILz NInYc3FIem:5dHigbY5pcWKrdHnvckBi\2GrboP0JIh2dWGwIFe0NFIh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygS2k2OD1zMT64O{DPxE1? Mn3UNlE1ODFzMUS=
A10 cells MVzGeY5kfGmxbjDhd5NigQ>? NUfhbHI1OzBizszN NIC4[GgzPCCqcoO= Mlq4SI94dnKnZ4XsZZRqd25ib3[gV4NlOiCvUl7BJIV5eHKnc4Ppc44hcW5icnH0JGEyOCClZXzsd{BifCB|MDD1UUBi\nSncjCyOEBpenNiYomgdZVidnSrdHH0bZZmKFKWLWDDVkBidmGueYPpdy=> M{C2cFIyPDBzMUG0
A10 cells NGPsc5RHfW6ldHnvckBie3OjeR?= NFPlSoE{OCEQvF2= NETmNG4zPCCqcoO= MoHFSI94diC{ZXf1cIF1cW:wIH;mJHBzcW1{IH3SUmEh\XiycnXzd4lwdiCrbjDyZZQhSTFyIHPlcIx{KGG2IEOwJJVOKGGodHXyJFI1KGi{czDifUByfWGwdHn0ZZRqfmViUmStVGNTKGGwYXz5d4l{ MYSyNVQxOTFzNB?=
A10 cells MWnGeY5kfGmxbjDhd5NigQ>? MX:zNEDPxE1? MXmyOEBpenN? NUW5W4dHTG:5boLl[5Vt[XSrb36gc4YhUW2yazDtVm5CKGW6cILld5Nqd25iaX6gdoF1KEFzMDDj[YxteyCjdDCzNEB2VSCjZoTldkAzPCCqcoOgZpkheXWjboTpeIF1cX[nIGLUMXBEWiCjbnHsfZNqew>? NXPqXGxvOjF2MEGxNVQ>
HepG2-A16-CD81 cells NEPVXW5HfW6ldHnvckBie3OjeR?= MlP5NVAh|ryP M2\SeW5QXkGUVFnTPkBCdnSrbXHsZZJq[WxibHn2[ZIhe3SjZ3WgZYN1cX[rdImgcYVie3W{ZXSgZZMh[SCpcnXheIVzKHSqYX6gOVAmKHKnZIXjeIlwdiCrbjDQcIF{dW:maYXtJJlw\WyraTDzZ4hqgm:wdDDhdoViKGmwIFjldGczNUFzNj3DSFgyKGOnbHzzJIF1KDFydV2gZ49ueG:3bnSgZ49v[2WwdILheIlwdixiZHX0[ZJucW6nZDDifUBqdW23bn:t[ox2d3Knc3PlcoNmNg>? MWmyNlA6PjFyMR?=
HepG2-A16-CD81 cells M321OGZ2dmO2aX;uJIF{e2G7 MmfrNVAh|ryP M{DEVm5QXkGUVFnTPkBCdnSrbXHsZZJq[WxibHn2[ZIhe3SjZ3WgZYN1cX[rdImgcYVie3W{ZXSgZZMhemWmdXP0bY9vKGmwIGDsZZNud2SrdX2gfY9mdGmrIIPjbIl7d262IHHy[YEhcW5iSHXwS|IuSTF4LVPEPFEh[2WubIOgZpkhcW2vdX7vMYZtfW:{ZYPj[Y5k\SxiYX7kJI1m\GmjbjDzZ4hqgm:wdDDzbZpmKGG2IEGweW0h[2:vcH;1coQh[2:wY3XueJJifGmxbjygTWM2OD1yLkW0PEDPxE1? MoPFNlIxQTZzMEG=
HepG2 cells M2f6R2Z2dmO2aX;uJIF{e2G7 M1X3TFExKM7:TR?= NHf2clA1KGh? NHrXXJdKdmO{ZXHz[UBqdiCDTWDLZYxxcGFicHjvd5Bpd3K7bHH0bY9vKGmwIHj1cYFvKEincFeyJINmdGy|IHH0JFExKHWPIHHmeIVzKDRiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrczDy[YxifGm4ZTD0c{B2dnS{ZXH0[YQh[2:wdILvcC=> NGjKcHYzOzB3OEGwOy=>
HepG2 cells NVzVSot1TnWwY4Tpc44h[XO|YYm= M2nvWVExKM7:TR?= M1zOd|QhcA>? M{PGfGlv[3KnYYPlJIlvKHSxdHHsJGFOWEujbIDoZUBt\X[nbDDpckBpfW2jbjDI[ZBIOiClZXzsd{BifCBzMDD1UUBi\nSncjC0JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZMhemWuYYTpeoUhfG9idX70doVifGWmIHPvcpRzd2x? MWmyN|A2QDFyNx?=
HepG2 cells NEXURXFHfW6ldHnvckBie3OjeR?= NYW1eY8xOjBizszN MVyyOEBpenN? MYDJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKEincFeyJINmdGy|IHHzd4V{e2WmIHHzJI1wenCqb3zv[4lk[WxiY3jhcodmeyCjdDCyNEB2VSCjZoTldkAzPCCqcoOgeZNqdmdiSH;lZ4h{fCB|M{K1PEB{fGGrbnnu[{BjgSCobIXvdoV{[2WwY3WgcYlkem:|Y3;wbYMh[W6jbInzbZM> NX3pPHBmOjNzOEKwPFg>
HT-29 cells NF;yc2VEgXSxdH;4bYNqfHliYYPzZZk> M1HBW|Q5KGi{cx?= NWHEVFBvS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUFRvMkmgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF05NjR3IN88US=> MXSyN|E5OjB6OB?=
HepG2 cells NXflcnNPS3m2b4TvfIlkcXS7IHHzd4F6 MWWyOEBpenN? MWPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjCyOEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUGxMlIzKM7:TR?= MVOyN|E5OjB6OB?=
HepG2 cells NEm1XHFEgXSxdH;4bYNqfHliYYPzZZk> NFfifnY1QCCqcoO= MYjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUiuN|Ih|ryP NWnNeFhGOjNzOEKwPFg>

... Click to View More Cell Line Experimental Data

Protocol

Solubility (25°C)

In vitro DMSO 40 mg/mL (107.58 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
3mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 371.81
Formula

C20H18NO4.Cl

CAS No. 633-65-8
Storage powder
in solvent
Synonyms Natural Yellow 18 chloride
Smiles COC1=C(C2=C[N+]3=C(C=C2C=C1)C4=CC5=C(C=C4CC3)OCO5)OC.[Cl-]

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03281096 Recruiting Drug: Berberine hydrochloride|Drug: Placebo Colorectal Adenomas xiaohua li|Xijing Hospital of Digestive Diseases August 29 2017 Phase 2|Phase 3
NCT02806999 Unknown status Drug: Berberine; Insulin|Drug: Insulin Hyperglycemia Guangdong Provincial People''s Hospital July 2016 Phase 4

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID