Berberine chloride (NSC 646666)

For research use only.

Catalog No.S2271 Synonyms: Natural Yellow 18 chloride

6 publications

Berberine chloride (NSC 646666) Chemical Structure

CAS No. 633-65-8

Berberine chloride (NSC 646666, Natural Yellow 18) is a quaternary ammonium salt from the group of isoquinoline alkaloids. Berberine activates caspase 3 and caspase 8, cleavage of poly ADP-ribose polymerase (PARP) and the release of cytochrome c. Berberine chloride decreases the expression of c-IAP1, Bcl-2 and Bcl-XL. Berberine chloride induces apoptosis with sustained phosphorylation of JNK and p38 MAPK, as well as generation of the ROS. Berberine chloride is a dual topoisomerase I and II inhibitor. Berberine chloride is also a potential autophagy modulator.

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10mM (1mL in DMSO) USD 90 In stock
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Selleck's Berberine chloride (NSC 646666) has been cited by 6 publications

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Biological Activity

Description Berberine chloride (NSC 646666, Natural Yellow 18) is a quaternary ammonium salt from the group of isoquinoline alkaloids. Berberine activates caspase 3 and caspase 8, cleavage of poly ADP-ribose polymerase (PARP) and the release of cytochrome c. Berberine chloride decreases the expression of c-IAP1, Bcl-2 and Bcl-XL. Berberine chloride induces apoptosis with sustained phosphorylation of JNK and p38 MAPK, as well as generation of the ROS. Berberine chloride is a dual topoisomerase I and II inhibitor. Berberine chloride is also a potential autophagy modulator.
Targets
Caspase-3 [1]
()
Caspase-8 [1]
()
PARP [1]
()
cytochrome c [1]
()
cIAP1 [1]
()
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MRC5 cells NEPVWFlHfW6ldHnvckBie3OjeR?= MkXmRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGNOXiCrbjDNVmM2KGOnbHzzJIJ6KHCuYYH1[UBz\WS3Y4Tpc44h[XO|YYmsJGlEPTB;MD62PEDPxE1? NWDEVI8xOTd{M{m1PVQ>
MRC5 cells NE\lcXlRem:uaX\ldoF1cW:wIHHzd4F6 NVyxeXZFOTBizszN NHrwXWI2PCCqcoO= MoPRTY5pcWKrdHnvckBw\iCKQ13WJJBzd2yrZnXyZZRqd25iaX6gUXJEPSClZXzsd{Bi\nSncjC1OEBpenNicH;zeE1qdm[nY4Tpc44h[XRiMUCgeW0h[nlicHzhdZVmKGG|c3H5 NWDIOW1JOTd{M{m1PVQ>
MRC5 cells MmLOVJJwdGmoZYLheIlwdiCjc4PhfS=> NGDpcZUyOCEQvF2= MmXCNlQhcA>? MVLJcohq[mm2aX;uJI9nKEiFTW[gdJJwdGmoZYLheIlwdiCrbjDNVmM2KGOnbHzzJIFnfGW{IEK0JIhzeyCyb4P0MYlv\mWldHnvckBifCBzMDD1UUBjgSCybHHxeYUh[XO|YYm= MXqxO|I{QTV7NB?=
Bel7402 cells NF\vZlZHfW6ldHnvckBie3OjeR?= Ml\aNVIhcA>? NHnIXGlKdmS3Y4Tpc44hd2ZiTFTMVkBxem:2ZXnuJIlvKGi3bXHuJGJmdDd2MEKgZ4VtdHNiYX\0[ZIhOTJiaILzJIJ6KFKWLWDDVkBie3OjeTDy[YxifGm4ZTD0c{Bkd262cn;s NHy5bFgyQTB7MEe2Oy=>
HepG2 cells MlvJSpVv[3Srb36gZZN{[Xl? M{T5OVExKHWpL33M NWPyfldoOTJiaB?= M3y0WWlv\HWldHnvckBw\iCORFzSJJBzd3SnaX6g[ZhxemW|c3nvckBqdiCqdX3hckBJ\XCJMjDj[YxteyCjdDCxNEB2\y:vTDDh[pRmeiBzMjDodpMh[nliZnzve{BkgXSxbXX0dpk> M3PIRlE6ODlyN{[3
KB cells NXnYRnN2S3m2b4TvfIlkcXS7IHHzd4F6 NHHhbHU4OiCq NInNT5pEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMSiClZXzsd{Bi\nSncjC3NkBpenNuIFnDOVA:Py5|MjFOwG0> M4jjZ|EyOTRzMUC1
HL60 cells NXjOWGJzSXCxcITvd4l{KGG|c3H5 NGrZdoE1QCCqcoO= MYXJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKEiONkCgZ4VtdHNiYX\0[ZIhPDhiaILzJJV{cW6pIHHucoV5cW5iVj3wdo9xcWSrdX2gbY9lcWSnIIP0ZYlvcW6pIHL5JGZCS1NiYX7hcJl{cXN? MVWyNFIzPzF2NB?=
A549 cells MkTIR5l1d3SxeHnjbZR6KGG|c3H5 MV3DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIJ6KFOUQjDhd5NigSxiSVO1NF03NjJ5IN88US=> MUiyNFU6PDh2OB?=
SKOV3 cells NIfyNINEgXSxdH;4bYNqfHliYYPzZZk> NXLsPZR2S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0uRVkOgZ4VtdHNiYomgV3JDKGG|c3H5MEBKSzVyPUG2MlQ1KM7:TR?= M{D4UVIxPTl2OES4
SK-MEL-2 cells M3uzbmN6fG:2b4jpZ4l1gSCjc4PhfS=> MWfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT{1OTUxvMjDj[YxteyCkeTDTVmIh[XO|YYmsJGlEPTB;MUOuO|Yh|ryP MnX4NlA2QTR6NEi=
HCT15 cells NFLKPIdEgXSxdH;4bYNqfHliYYPzZZk> MU\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3QyPSClZXzsd{BjgSCVUlKgZZN{[XluIFnDOVA:OTZwNUmg{txO NF7lXVYzODV7NEi0PC=>
CEM cells M4TESGN6fG:2b4jpZ4l1gSCjc4PhfS=> NELZUHU1QCCqcoO= MkCyR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gR2VOKGOnbHzzJIV5eHKnc4Ppcoch\3KnZX6g[ox2d3Knc3PlcpQheHKxdHXpckBi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBESzVyPUKuNFkh|ryP MXqyNVI6PTh7MR?=
human CEM cells M4LTNGZ2dmO2aX;uJIF{e2G7 NFfoXI44KGSjeYO= MVzBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDCwMlA2KE2RSTDIeY1idiCrbX31co9l\W[rY3nlcoN6KH[rcoXzJFEhVkx2LkOgbY5n\WO2ZXSgbY4hcHWvYX6gR2VOKGOnbHzzJIV5eHKnc4Ppcoch\3KnZX6g[ox2d3Knc3PlcpQheHKxdHXpckBie3Onc4Pl[EBieyCyMkSgZY51cWenbjDwdo9lfWO2aX;uJI1m[XO3cnXkJFch\GG7czDwc5N1KGmwZnXjeIlwdiCkeTDFUGlUSSxiRVO1NF0xNjF|IN88US=> NIXBcm0zOTJ7NUi5NS=>
SKN cells NHGxfHBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVy3NkBp Mo\iS5Jwf3SqIHnubIljcXSrb36gZYdicW6|dDDoeY1idiCVS16gZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiR1m1NF0yPS56ODFOwG0> NFP2dFYzOTRyMUGxOC=>
RKN cells MlfZS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NIXJPHc1QCCqcoO= MYfHdo94fGhiaX7obYJqfGmxbjDh[4FqdnO2IHj1cYFvKFKNTjDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCJSUWwQVQ6NjZizszN MYKyNVQxOTFzNB?=
G402 cells NGKwc5dIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVi0PEBpenN? MmPzS5Jwf3SqIHnubIljcXSrb36gZYdicW6|dDDoeY1idiCJNECyJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFfJOVA:OTFwOEeg{txO MnThNlE1ODFzMUS=
A10 cells NXq3S3FTTnWwY4Tpc44h[XO|YYm= M3H2UlMxKM7:TR?= M{jXblI1KGi{cx?= NGLE[llFd3ewcnXneYxifGmxbjDv[kBU[2R{IH3SUmEh\XiycnXzd4lwdiCrbjDyZZQhSTFyIHPlcIx{KGG2IEOwJJVOKGGodHXyJFI1KGi{czDifUByfWGwdHn0ZZRqfmViUmStVGNTKGGwYXz5d4l{ NYi5elh4OjF2MEGxNVQ>
A10 cells Mn3TSpVv[3Srb36gZZN{[Xl? MVyzNEDPxE1? M2K4ZVI1KGi{cx?= MUHEc5dvKHKnZ4XsZZRqd25ib3[gVJJqdTJibWLORUBmgHC{ZYPzbY9vKGmwIILheEBCOTBiY3XscJMh[XRiM{CgeW0h[W[2ZYKgNlQhcHK|IHL5JJF2[W62aYTheIl3\SCUVD3QR3Ih[W6jbInzbZM> NEC0d48zOTRyMUGxOC=>
A10 cells NYXlOpBHTnWwY4Tpc44h[XO|YYm= MVizNEDPxE1? NEnBTGwzPCCqcoO= M2O1OmRwf26{ZXf1cIF1cW:wIH;mJGlueGtibWLORUBmgHC{ZYPzbY9vKGmwIILheEBCOTBiY3XscJMh[XRiM{CgeW0h[W[2ZYKgNlQhcHK|IHL5JJF2[W62aYTheIl3\SCUVD3QR3Ih[W6jbInzbZM> MYqyNVQxOTFzNB?=
HepG2-A16-CD81 cells M4PCcWZ2dmO2aX;uJIF{e2G7 NETzboEyOCEQvF2= M3HWXm5QXkGUVFnTPkBCdnSrbXHsZZJq[WxibHn2[ZIhe3SjZ3WgZYN1cX[rdImgcYVie3W{ZXSgZZMh[SCpcnXheIVzKHSqYX6gOVAmKHKnZIXjeIlwdiCrbjDQcIF{dW:maYXtJJlw\WyraTDzZ4hqgm:wdDDhdoViKGmwIFjldGczNUFzNj3DSFgyKGOnbHzzJIF1KDFydV2gZ49ueG:3bnSgZ49v[2WwdILheIlwdixiZHX0[ZJucW6nZDDifUBqdW23bn:t[ox2d3Knc3PlcoNmNg>? MofuNlIxQTZzMEG=
HepG2-A16-CD81 cells MkTwSpVv[3Srb36gZZN{[Xl? M1niOVExKM7:TR?= MVvOU3ZCWlSLU{qgRY51cW2jbHHybYFtKGyrdnXyJJN1[WenIHHjeIl3cXS7IH3lZZN2emWmIHHzJJJm\HWldHnvckBqdiCSbHHzcY9lcXWvIInv[YxqcSC|Y3jpfo9vfCCjcnXhJIlvKEincFeyMWEyPi2FREixJINmdGy|IHL5JIludXWwbz3mcJVwemW|Y3XuZ4UtKGGwZDDt[YRq[W5ic3PobZpwdnRic3n6[UBifCBzMIXNJINwdXCxdX7kJINwdmOnboTyZZRqd25uIFnDOVA:OC53NEig{txO NF7FeZEzOjB7NkGwNS=>
HepG2 cells MknVSpVv[3Srb36gZZN{[Xl? NXPPfo1VOTBizszN NX\0ZXlRPCCq M2[1bmlv[3KnYYPlJIlvKEGPUFvhcJBp[SCyaH;zdIhwenmuYYTpc44hcW5iaIXtZY4hUGWyR{KgZ4VtdHNiYYSgNVAhfU1iYX\0[ZIhPCCqcoOgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m|IILlcIF1cX[nIITvJJVvfHKnYYTl[EBkd262cn;s MnfXNlMxPThzMEe=
HepG2 cells MYLGeY5kfGmxbjDhd5NigQ>? M2TIN|ExKM7:TR?= NXjxOnBtPCCq Mn64TY5kemWjc3WgbY4hfG:2YXygRW1RU2GucHjhJIxmfmWuIHnuJIh2dWGwIFjldGczKGOnbHzzJIF1KDFyIIXNJIFnfGW{IESgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpd{Bz\WyjdHn2[UB1dyC3boTy[YF1\WRiY3;ueJJwdA>? NWq0WGpNOjNyNUixNFc>
HepG2 cells NWf1fGdiTnWwY4Tpc44h[XO|YYm= NEHaboUzOCEQvF2= M3r0UVI1KGi{cx?= MmjwTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCKZYDHNkBk\WyuczDhd5Nme3OnZDDhd{Bud3KyaH;sc4dq[2GuIHPoZY5o\XNiYYSgNlAhfU1iYX\0[ZIhOjRiaILzJJV{cW6pIFjv[YNpe3RiM{OyOVghe3SjaX7pcoch[nliZnz1c5Jme2OnbnPlJI1q[3Kxc3PvdIlkKGGwYXz5d4l{ MUSyN|E5OjB6OB?=
HT-29 cells NXHW[3NsS3m2b4TvfIlkcXS7IHHzd4F6 NXHxT|VYPDhiaILz MlmxR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTHQuOjliY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME24MlQ2KM7:TR?= MUOyN|E5OjB6OB?=
HepG2 cells NXL1clV3S3m2b4TvfIlkcXS7IHHzd4F6 NWHnbpM4OjRiaILz NX;lPYRtS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWyR{KgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yOS5{MjFOwG0> MkXONlMyQDJyOEi=
HepG2 cells MYXDfZRwfG:6aXPpeJkh[XO|YYm= NFfBSnQ1QCCqcoO= M{jLUmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9PE4{OiEQvF2= Mn3YNlMyQDJyOEi=

... Click to View More Cell Line Experimental Data

Protocol

Solubility (25°C)

In vitro DMSO 40 mg/mL (107.58 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
3mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 371.81
Formula

C20H18NO4.Cl

CAS No. 633-65-8
Storage powder
in solvent
Synonyms Natural Yellow 18 chloride
Smiles COC1=C(C2=C[N+]3=C(C=C2C=C1)C4=CC5=C(C=C4CC3)OCO5)OC.[Cl-]

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03281096 Unknown status Drug: Berberine hydrochloride|Drug: Placebo Colorectal Adenomas xiaohua li|Xijing Hospital of Digestive Diseases August 29 2017 Phase 2|Phase 3
NCT02806999 Unknown status Drug: Berberine; Insulin|Drug: Insulin Hyperglycemia Guangdong Provincial People''s Hospital July 2016 Phase 4

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID