Berberine chloride (NSC 646666)

For research use only.

Catalog No.S2271 Synonyms: Natural Yellow 18 chloride

6 publications

Berberine chloride (NSC 646666) Chemical Structure

CAS No. 633-65-8

Berberine chloride (NSC 646666, Natural Yellow 18) is a quaternary ammonium salt from the group of isoquinoline alkaloids. Berberine activates caspase 3 and caspase 8, cleavage of poly ADP-ribose polymerase (PARP) and the release of cytochrome c. Berberine chloride decreases the expression of c-IAP1, Bcl-2 and Bcl-XL. Berberine chloride induces apoptosis with sustained phosphorylation of JNK and p38 MAPK, as well as generation of the ROS. Berberine chloride is a dual topoisomerase I and II inhibitor. Berberine chloride is also a potential autophagy modulator.

Selleck's Berberine chloride (NSC 646666) has been cited by 6 publications

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Biological Activity

Description Berberine chloride (NSC 646666, Natural Yellow 18) is a quaternary ammonium salt from the group of isoquinoline alkaloids. Berberine activates caspase 3 and caspase 8, cleavage of poly ADP-ribose polymerase (PARP) and the release of cytochrome c. Berberine chloride decreases the expression of c-IAP1, Bcl-2 and Bcl-XL. Berberine chloride induces apoptosis with sustained phosphorylation of JNK and p38 MAPK, as well as generation of the ROS. Berberine chloride is a dual topoisomerase I and II inhibitor. Berberine chloride is also a potential autophagy modulator.
Targets
Caspase-3 [1]
()
Caspase-8 [1]
()
PARP [1]
()
cytochrome c [1]
()
cIAP1 [1]
()
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MRC5 cells MUXGeY5kfGmxbjDhd5NigQ>? Mn7PRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGNOXiCrbjDNVmM2KGOnbHzzJIJ6KHCuYYH1[UBz\WS3Y4Tpc44h[XO|YYmsJGlEPTB;MD62PEDPxE1? NX;XUGxiOTd{M{m1PVQ>
MRC5 cells NI\jcJZRem:uaX\ldoF1cW:wIHHzd4F6 MmK3NVAh|ryP NFO0Tlc2PCCqcoO= M4iyXWlvcGmkaYTpc44hd2ZiSFPNWkBxem:uaX\ldoF1cW:wIHnuJG1TSzViY3XscJMh[W[2ZYKgOVQhcHK|IIDvd5QucW6oZXP0bY9vKGG2IEGwJJVOKGK7IIDsZZF2\SCjc4PhfS=> NFzjZ2syPzJ|OUW5OC=>
MRC5 cells M2DrbXBzd2yrZnXyZZRqd25iYYPzZZk> MYOxNEDPxE1? MV2yOEBp NWHvbVk2UW6qaXLpeIlwdiCxZjDIR21XKHC{b3zp[oVz[XSrb36gbY4hVVKFNTDj[YxteyCjZoTldkAzPCCqcoOgdI9{fC2rbn\lZ5Rqd25iYYSgNVAhfU1iYomgdIxieXWnIHHzd4F6 MWKxO|I{QTV7NB?=
Bel7402 cells NUTwS3FsTnWwY4Tpc44h[XO|YYm= NV\ZXXk1OTJiaB?= MXrJcoR2[3Srb36gc4YhVESOUjDwdo91\WmwIHnuJIh2dWGwIFLlcFc1ODJiY3XscJMh[W[2ZYKgNVIhcHK|IHL5JHJVNVCFUjDhd5NigSC{ZXzheIl3\SC2bzDjc451em:u MlH6NVkxQTB5Nke=
HepG2 cells M2\tbWZ2dmO2aX;uJIF{e2G7 MmT0NVAhfWdxbVy= MWSxNkBp MorDTY5lfWO2aX;uJI9nKEyGTGKgdJJwfGWrbjDlfJBz\XO|aX;uJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGG2IEGwJJVoN22OIHHmeIVzKDF{IHjyd{BjgSCobH;3JIN6fG:vZYTyfS=> NGewVHQyQTB7MEe2Oy=>
KB cells NVfQS3pKS3m2b4TvfIlkcXS7IHHzd4F6 NEfNO3k4OiCq MYrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLRkBk\WyuczDh[pRmeiB5MjDodpMtKEmFNUC9O{4{OiEQvF2= MnTrNVEyPDFzMEW=
HL60 cells MmmyRZBweHSxc3nzJIF{e2G7 NUK0cJlxPDhiaILz Ml;GTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCKTE[wJINmdGy|IHHmeIVzKDR6IHjyd{B2e2mwZzDhco5mgGmwIG[tdJJweGmmaYXtJIlw\GmmZTDzeIFqdmmwZzDifUBHSUOVIHHuZYx6e2m| MmO3NlAzOjdzNES=
A549 cells MUPDfZRwfG:6aXPpeJkh[XO|YYm= M{jXZmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[nliU2LCJIF{e2G7LDDJR|UxRTZwMkeg{txO M4rScVIxPTl2OES4
SKOV3 cells NYnLfpoxS3m2b4TvfIlkcXS7IHHzd4F6 MXHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT29XOyClZXzsd{BjgSCVUlKgZZN{[XluIFnDOVA:OTZwNESg{txO NXjOZZBqOjB3OUS4OFg>
SK-MEL-2 cells NXXnXW5oS3m2b4TvfIlkcXS7IHHzd4F6 NVP2XnZRS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0tvTVXMMVIh[2WubIOgZpkhW1KEIHHzd4F6NCCLQ{WwQVE{Njd4IN88US=> MkDnNlA2QTR6NEi=
HCT15 cells NVH6WWF2S3m2b4TvfIlkcXS7IHHzd4F6 NGfZZ|hEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzNTDj[YxteyCkeTDTVmIh[XO|YYmsJGlEPTB;MU[uOVkh|ryP M1vrTVIxPTl2OES4
CEM cells MV\DfZRwfG:6aXPpeJkh[XO|YYm= MlvnOFghcHK| Mn\SR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gR2VOKGOnbHzzJIV5eHKnc4Ppcoch\3KnZX6g[ox2d3Knc3PlcpQheHKxdHXpckBi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBESzVyPUKuNFkh|ryP MoLUNlEzQTV6OUG=
human CEM cells MY\GeY5kfGmxbjDhd5NigQ>? MkLOO{Bl[Xm| NFH6XW1CdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEAxNjB3IF3PTUBJfW2jbjDpcY12dm:mZX\pZ4lmdmO7II\pdpV{KDFiTly0MlMhcW6oZXP0[YQhcW5iaIXtZY4hS0WPIHPlcIx{KGW6cILld5NqdmdiZ4Ll[Y4h\my3b4Lld4NmdnRicILveIVqdiCjc4Pld5Nm\CCjczDwNlQh[W62aXflckBxem:mdXP0bY9vKG2nYYP1doVlKDdiZHH5d{Bxd3O2IHnu[oVkfGmxbjDifUBGVEmVQTygSWM2OD1yLkGzJO69VQ>? NILZdZUzOTJ7NUi5NS=>
SKN cells M2ezZmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1nsZlczKGh? NWLGPItFT3Kxd4ToJIlvcGmkaYTpc44h[WejaX7zeEBpfW2jbjDTT24h[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygS2k2OD1zNT64PEDPxE1? NWPnNIlzOjF2MEGxNVQ>
RKN cells Ml\3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MoTDOFghcHK| NHu4XJhIem:5dHigbY5pcWKrdHnvckBi\2GrboP0JIh2dWGwIGLLUkBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDHTVUxRTR7Lk[g{txO M37Hb|IyPDBzMUG0
G402 cells NF7jVGFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3[4NFQ5KGi{cx?= M13SeGdzd3e2aDDpcohq[mm2aX;uJIFo[Wmwc4SgbJVu[W5iR{SwNkBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDHTVUxRTFzLki3JO69VQ>? NFi5cnMzOTRyMUGxOC=>
A10 cells NEPZXmRHfW6ldHnvckBie3OjeR?= M1P6eVMxKM7:TR?= Ml;jNlQhcHK| NEHBV2tFd3ewcnXneYxifGmxbjDv[kBU[2R{IH3SUmEh\XiycnXzd4lwdiCrbjDyZZQhSTFyIHPlcIx{KGG2IEOwJJVOKGGodHXyJFI1KGi{czDifUByfWGwdHn0ZZRqfmViUmStVGNTKGGwYXz5d4l{ NWjxb5lGOjF2MEGxNVQ>
A10 cells Mk\PSpVv[3Srb36gZZN{[Xl? NGD3PGg{OCEQvF2= MlHwNlQhcHK| MWDEc5dvKHKnZ4XsZZRqd25ib3[gVJJqdTJibWLORUBmgHC{ZYPzbY9vKGmwIILheEBCOTBiY3XscJMh[XRiM{CgeW0h[W[2ZYKgNlQhcHK|IHL5JJF2[W62aYTheIl3\SCUVD3QR3Ih[W6jbInzbZM> MVSyNVQxOTFzNB?=
A10 cells MWLGeY5kfGmxbjDhd5NigQ>? NH3Rdlg{OCEQvF2= MXqyOEBpenN? NHrzR2JFd3ewcnXneYxifGmxbjDv[kBKdXCtIH3SUmEh\XiycnXzd4lwdiCrbjDyZZQhSTFyIHPlcIx{KGG2IEOwJJVOKGGodHXyJFI1KGi{czDifUByfWGwdHn0ZZRqfmViUmStVGNTKGGwYXz5d4l{ NXW2ZYhiOjF2MEGxNVQ>
HepG2-A16-CD81 cells MmewSpVv[3Srb36gZZN{[Xl? MUixNEDPxE1? MW\OU3ZCWlSLU{qgRY51cW2jbHHybYFtKGyrdnXyJJN1[WenIHHjeIl3cXS7IH3lZZN2emWmIHHzJIEh\3KnYYTldkB1cGGwIEWwKUBz\WS3Y4Tpc44hcW5iUHzhd41w\Gm3bTD5c4VtcWlic3PobZpwdnRiYYLlZUBqdiCKZYDHNk1COTZvQ1S4NUBk\WyuczDheEAyOHWPIHPvcZBwfW6mIHPvcoNmdnS{YYTpc44tKGSndHXycYlv\WRiYomgbY1ufW6xLX\seY9z\XOlZX7j[U4> NXWxOFV6OjJyOU[xNFE>
HepG2-A16-CD81 cells NFnUTnZHfW6ldHnvckBie3OjeR?= Ml;RNVAh|ryP NXfvSnp5Vk:YQWLUTXM7KEGwdHntZYxiemmjbDDsbZZmeiC|dHHn[UBi[3Srdnn0fUBu\WG|dYLl[EBieyC{ZXT1Z5Rqd25iaX6gVIxie22xZHn1cUB6d2WuaXmgd4NpcXqxboSgZZJm[SCrbjDI[ZBIOi2DMU[tR2Q5OSClZXzsd{BjgSCrbX31co8u\my3b4Lld4NmdmOnLDDhcoQhdWWmaXHuJJNkcGm8b370JJNqgmViYYSgNVB2VSClb33wc5Vv\CClb37j[Y51emG2aX;uMEBKSzVyPUCuOVQ5KM7:TR?= M4jkU|IzODl4MUCx
HepG2 cells MorvSpVv[3Srb36gZZN{[Xl? NVHiUYVSOTBizszN M3rofVQhcA>? NX3QVlFnUW6lcnXhd4UhcW5iQV3QT4FteGijIIDoc5NxcG:{eXzheIlwdiCrbjDoeY1idiCKZYDHNkBk\WyuczDheEAyOCC3TTDh[pRmeiB2IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYOgdoVt[XSrdnWgeI8hfW62cnXheIVlKGOxboTyc4w> NV22eFkxOjNyNUixNFc>
HepG2 cells MUPGeY5kfGmxbjDhd5NigQ>? NFWyfGoyOCEQvF2= M3LRN|QhcA>? Ml\YTY5kemWjc3WgbY4hfG:2YXygRW1RU2GucHjhJIxmfmWuIHnuJIh2dWGwIFjldGczKGOnbHzzJIF1KDFyIIXNJIFnfGW{IESgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpd{Bz\WyjdHn2[UB1dyC3boTy[YF1\WRiY3;ueJJwdA>? MojyNlMxPThzMEe=
HepG2 cells M{\ZS2Z2dmO2aX;uJIF{e2G7 NV7UXWpzOjBizszN MmjpNlQhcHK| NGLzOXNKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJIh2dWGwIFjldGczKGOnbHzzJIF{e2W|c3XkJIF{KG2xcoDoc4xw\2mlYXygZ4hidmenczDheEAzOCC3TTDh[pRmeiB{NDDodpMhfXOrbnegTI9m[2i|dDCzN|I2QCC|dHHpcolv\yCkeTDmcJVwemW|Y3XuZ4UhdWmlcn;zZ49xcWNiYX7hcJl{cXN? MVGyN|E5OjB6OB?=
HT-29 cells NX\OXoVSS3m2b4TvfIlkcXS7IHHzd4F6 NWXheIlDPDhiaILz NFjCSW5EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJXC1{OTDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVgvPDVizszN NEjTZZQzOzF6MkC4PC=>
HepG2 cells MXvDfZRwfG:6aXPpeJkh[XO|YYm= M1HnflI1KGi{cx?= Moj4R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xNU4zOiEQvF2= MkjKNlMyQDJyOEi=
HepG2 cells NFrNSHREgXSxdH;4bYNqfHliYYPzZZk> NFnaZmo1QCCqcoO= M{i4N2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9PE4{OiEQvF2= MnfYNlMyQDJyOEi=

... Click to View More Cell Line Experimental Data

Protocol

Solubility (25°C)

In vitro DMSO 40 mg/mL (107.58 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
3mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 371.81
Formula

C20H18NO4.Cl

CAS No. 633-65-8
Storage powder
in solvent
Synonyms Natural Yellow 18 chloride
Smiles COC1=C(C2=C[N+]3=C(C=C2C=C1)C4=CC5=C(C=C4CC3)OCO5)OC.[Cl-]

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03281096 Unknown status Drug: Berberine hydrochloride|Drug: Placebo Colorectal Adenomas xiaohua li|Xijing Hospital of Digestive Diseases August 29 2017 Phase 2|Phase 3
NCT02806999 Unknown status Drug: Berberine; Insulin|Drug: Insulin Hyperglycemia Guangdong Provincial People''s Hospital July 2016 Phase 4

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID