Ascomycin (FK520)

Catalog No.S7411 Synonyms: FR 900520, Immunomycin

For research use only.

Ascomycin (FK520, FR 900520, Immunomycin), an FK-506 analog, is a neutral macrolide immunosuppressant, which prevents rejection after an organ transplant. Phase 3.

Ascomycin (FK520) Chemical Structure

CAS No. 104987-12-4

Selleck's Ascomycin (FK520) has been cited by 2 Publications

Purity & Quality Control

Choose Selective Anti-infection Inhibitors

Biological Activity

Description Ascomycin (FK520, FR 900520, Immunomycin), an FK-506 analog, is a neutral macrolide immunosuppressant, which prevents rejection after an organ transplant. Phase 3.
Targets
calcineurin phosphatase [2]
49 nM
In vitro

Ascomycin, isolated from Streptomyces, suppresses immune response in vitro with IC50 of 0.55 nM for mouse mixed lymphocyte. [1] Ascomycin inhibits calcineurin phosphatase with IC50 of 49 nM through formation of an FKBP12-FK520-calcineurin ternary complex. FK520 also accelerate the rate of nerve regeneration, and promotes neurite outgrowth. [2] Ascomycin shows antimalarial effects via inhibition of the chaperone activity of this bifunctional protein. [3]

In vivo Ascomycin (3.2 mg/kg, i.m.) clearly prolongs skin allograft survival in rats. [1] Ascomycin, at 50 or 100 μM, produces anticonvulsant effect against picrotoxin-induced seizures when infused into the rat hippocampus. [4]

Protocol (from reference)

Animal Research:[1]
  • Animal Models: Recipient WKA rats transplanted with F344 skin allografts.
  • Dosages: ~32 mg/kg 5 days a week.
  • Administration: i.m.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 792.01
Formula

C43H69NO12

CAS No. 104987-12-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCC1C=C(CC(CC(C2C(CC(C(O2)(C(=O)C(=O)N3CCCCC3C(=O)OC(C(C(CC1=O)O)C)C(=CC4CCC(C(C4)OC)O)C)O)C)OC)OC)C)C

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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