ABT-737

For research use only.

Catalog No.S1002

319 publications

ABT-737 Chemical Structure

CAS No. 852808-04-9

ABT-737 is a BH3 mimetic inhibitor of Bcl-xL, Bcl-2 and Bcl-w with EC50 of 78.7 nM, 30.3 nM and 197.8 nM in cell-free assays, respectively; no inhibition observed against Mcl-1, Bcl-B or Bfl-1. ABT-737 induces mitochondrial pathway apoptosis and mitophagy. Phase 2.

Selleck's ABT-737 has been cited by 319 publications

Purity & Quality Control

Choose Selective Bcl-2 Inhibitors

Biological Activity

Description ABT-737 is a BH3 mimetic inhibitor of Bcl-xL, Bcl-2 and Bcl-w with EC50 of 78.7 nM, 30.3 nM and 197.8 nM in cell-free assays, respectively; no inhibition observed against Mcl-1, Bcl-B or Bfl-1. ABT-737 induces mitochondrial pathway apoptosis and mitophagy. Phase 2.
Features First-generation inhibitor of anti-apoptotic Bcl-2 proteins.
Targets
Bcl-2 [1]
(Cell-free assay)
Bcl-xL [1]
(Cell-free assay)
Bcl-w [1]
(Cell-free assay)
Bcl-B [1]
(Cell-free assay)
30.3 nM(EC50) 78.7 nM(EC50) 197.8 nM(EC50) 1.82 μM(EC50)
In vitro

ABT-737 shows low activity to Bcl-B and no effects to Mcl-1 and BFL-1. ABT-737 is sensitive to HL60, KG1 and NB4 cells with IC50 of 50 nM, 80 nM and 80 nM, respectively. ABT-737 induces apoptosis in HL60 cells, which due to decreased Bcl-2/Bax heterodimerization and has no effect on cell cycle distribution. ABT-737 also induces cytochrome c release from purified mitochondria and promotes conformational changes in Bax that are associated with apoptosis. [1] Resistant cells (Hela and MCF-7) can be sensitized to ABT-737 by approaches that down-regulate, destabilize, or inactivate Mcl-1. ABT-737 also causes Bax/BAK-dependent cytochrome c release only when Mcl-1 has been neutralized. [2] ABT-737 displaces Bim from Bcl2's BH3-binding pocket, allowing Bim to activate Bax, induce mitochondrial permeabilization, and rapidly commit the primary chronic lymphocytic leukemia (CLL) cells to death. [3] Knockdown of Mcl-1 with siRNA sensitizes two resistant SCLC cell lines H196 and DMS114 to ABT-737 by enhancing the induction of apoptosis. Likewise, up-regulation of Noxa sensitizes H196 cells to ABT-737. ABT-737 inhibits proliferation and induces apoptosis in many SCLC cell lines including NCI-H889, NCI-H1963, NCI-H1417, NCI-H146 and etc. Bcl-2 and Noxa may contribute mechanistically to the cellular response to ABT-737 in NCI-H146 cells. [4] A recent study shows that ABT-737 significantly induces apoptosis in HTLV-1 infected T-cell lines as well as in fresh ATLL cells. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
OCI-Ly1  NIfaV2xE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MnHTNlUxKG6PwrC= Ml;jO|IhcA>? Mki4SG1UVw>? M{S3OINifXOnZDC5O{UhdG:|czDv[kB3cWGkaXzpeJkhcW5iY3XscJMhfHKjboPm[YN1\WRid3n0bEBDS0x4IIPpVm5C MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjZ3N{K4PEc,OjZ4NUeyPFg9N2F-
KG1a Mly2R4VtdCCYaXHibYxqfHliQYPzZZk> MoDxNE0yOCEQvF2= NXH3NGJWOjRiaB?= NI\4e4pFVVOR M{\HXmlEPTB;Nz62PEDPxE1uIHTlZ5Jm[XOnczDj[YxtKH[rYXLpcIl1gSCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV3MkexNkc,OjZ3NUK3NVI9N2F-
Kasumi-1 MV7D[YxtKF[rYXLpcIl1gSCDc4PhfS=> MnfjNE0yOCEQvF2= MlK1NlQhcA>? M4DweWROW09? MUfJR|UxRTRwOEeg{txONCCmZXPy[YF{\XNiY3XscEB3cWGkaXzpeJkhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> M1\ZTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUWyO|EzLz5{NkW1NlcyOjxxYU6=
KG1a MVzBdI9xfG:|aYOgRZN{[Xl? MU[wMVExKM7:TR?= NUX3PVY2OjRiaB?= NHrIPYFFVVOR NUTNWlZycW6mdXPld{Bk\WyuIHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= Mo\1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3NUK3NVIoRjJ4NUWyO|EzRC:jPh?=
Kasumi-1 NFW3RpNCeG:ydH;zbZMhSXO|YYm= MWewMVExKM7:TR?= NI\OOnAzPCCq MmnNSG1UVw>? NFWxcHFqdmS3Y3XzJINmdGxiYYDvdJRwe2m|IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ NH\Z[5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW1NlcyOid-Mk[1OVI4OTJ:L3G+
MC-3  NILSSmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmDJOU8yOC9{MDFOwG0> MkPMNlQhcA>? MlXxSG1UVw>? MoP0bY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NF:3bXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkS0O|YyPSd-Mk[0OFc3OTV:L3G+
HN22  Moj6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnHQNk42NzdwNT:yNk42KM7:TR?= MkDsNlQhcA>? NFvnRmNFVVOR NFrUUXlqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjR2N{[xOUc,OjZ2NEe2NVU9N2F-
MC-3  M4D0b2Fxd3C2b4Ppd{BCe3OjeR?= M3HPT|UwOTBxMkCg{txO Mn[3NlQhcA>? NEfxcXdFVVOR MVfpcoR2[2W|IHPhd5Bie2VvbXXkbYF1\WRiYYDvdJRwe2m| M2rzWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NES3OlE2Lz5{NkS0O|YyPTxxYU6=
HN22  NITEV2pCeG:ydH;zbZMhSXO|YYm= MX[yMlUwPy53L{KyMlUh|ryP MnjwNlQhcA>? MnvCSG1UVw>? MVfpcoR2[2W|IHPhd5Bie2VvbXXkbYF1\WRiYYDvdJRwe2m| MmT2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2NEe2NVUoRjJ4NES3OlE2RC:jPh?=
MOLT-4 NXflXGxYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1XKR|ExNTVyMECgcm0> M4fBUFczKGh? NHW2V3hFVVOR M2j6NWlEPTB;MD6xPVgh|ryP MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7MkOzNkc,OjZ|OUKzN|I9N2F-
RS4;11 NIXZNHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGXHWZoyOC13MECwJI5O NYTkdI45PzJiaB?= NYjzW4FMTE2VTx?= Mo\VTWM2OD1yLkCwNkDPxE1? M3nYSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{myN|MzLz5{NkO5NlM{OjxxYU6=
JURKAT MmrQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\4SXVuOTBvNUCwNEBvVQ>? NXnQXY9GPzJiaB?= NGi4eI5FVVOR NGjTV3JKSzVyPU[2JO69VQ>? NXPBW3ZmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVI{OzJpPkK2N|kzOzN{PD;hQi=>
CEM R MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIXW[mEyOC13MECwJI5O NELjOI04OiCq M37JNmROW09? M1e3d2lEPTB;NT60JO69VQ>? NWf0XWxwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVI{OzJpPkK2N|kzOzN{PD;hQi=>
CEM S M{\IUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3PQV|ExNTVyMECgcm0> M2TVfFczKGh? NH\qWGFFVVOR NXf3UZlbUUN3ME2xNk4yKM7:TR?= NUDJNXBZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVI{OzJpPkK2N|kzOzN{PD;hQi=>
MOLT-4 MoXZRZBweHSxc3nzJGF{e2G7 NGH2ZXAyOC1zMECwJI5O MmXBNlQhcA>? M{P1[mROW09? MY\jZZV{\XNidHjlJINt\WG4YXflJI9nKEKlbD2yJIFv\CC2aHWg[I94dnKnZ4XsZZRqd25ib3[gRoNtNXiOIHHu[EBO[2xvMR?= MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7MkOzNkc,OjZ|OUKzN|I9N2F-
CEM S Mn\QRZBweHSxc3nzJGF{e2G7 M1GyclExNTFyMECgcm0> MX:yOEBp M3vSeWROW09? NGTWeXVk[XW|ZYOgeIhmKGOuZXH2ZYdmKG:oIFLjcE0zKGGwZDD0bIUh\G:5boLl[5Vt[XSrb36gc4YhSmOuLYjMJIFv\CCPY3ytNS=> M4izbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{myN|MzLz5{NkO5NlM{OjxxYU6=
JURKAT MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NI\uTmIyODBvMUCwNEBvVQ>? NX7YZZU2PDhiaB?= NH73SYhFVVOR MVrJR|UxRTl3NdMxPU4{KG6P NV\rR2RuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xO|IzPjlpPkK2NVczOjZ7PD;hQi=>
LOUCY M2nsWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYmxNFAuOTByMDDuUS=> NGrDNnE1QCCq NU\FcY43TE2VTx?= NH7KVVRKSzVyPUOyMljDuTFyLkmgcm0> NVXOcml7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xO|IzPjlpPkK2NVczOjZ7PD;hQi=>
WM-115 NVm2bmhrS2WubDDWbYFjcWyrdImgRZN{[Xl? MW[xNFDDqG6P MVq3NkBp MUPlcohidmOnczDjeZJkfW2rbj3pcoR2[2WmIHHueIkue3W{dnn2ZYzDqA>? NF3ySlQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkGxOlc4Pid-Mk[xNVY4PzZ:L3G+
B16 M3LP[GNmdGxiVnnhZoltcXS7IFHzd4F6 M{nUOFExOMLibl2= NGjqbY84OiCq MorD[Y5p[W6lZYOgZ5Vz[3WvaX6tbY5lfWOnZDDhcpRqNXO3co\peoFtyqB? NHfZWIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkGxOlc4Pid-Mk[xNVY4PzZ:L3G+
HL-60  NUHMVmlCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWi3NkBp M3rGWmlEPTEEoE2gNVAvPyCwTR?= NILROpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC0OVYxQSd-Mk[wOFU3ODl:L3G+
MOLM-13  NFLObFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWi3NkBp M17WRWlEPTEEoE2gNlcvQSCwTR?= M3LYN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MES1OlA6Lz5{NkC0OVYxQTxxYU6=
OCI-AML3 NWHEfFBCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHT0XYg4OiCq NFHRcINKSzVywrC9JFE6PTBibl2= NWrxdFV5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wOFU3ODlpPkK2NFQ2PjB7PD;hQi=>
BCWM.1 M4jLO2Fxd3C2b4Ppd{BCe3OjeR?= M3LEc|AuOS54IN88US=> Mn;UNlQhcA>? NIjyW45qdmS3Y3XzJINmdGxiYYDvdJRwe2m| MoXuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6OUOyPVAoRjJ3OEmzNlkxRC:jPh?=
MWCL-1 MmHJRZBweHSxc3nzJGF{e2G7 MXOwMVEvPiEQvF2= MYGyOEBp NFy0dINqdmS3Y3XzJINmdGxiYYDvdJRwe2m| M2jjS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEmzNlkxLz5{NUi5N|I6ODxxYU6=
MM.1s M4DnVGFxd3C2b4Ppd{BCe3OjeR?= MmrENE0yNjZizszN M1\idlI1KGh? NX3LZoRncW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh7M{K5NEc,OjV6OUOyPVA9N2F-
HCT116 NYi1UGU5TnWwY4Tpc44hSXO|YYm= M2XZWlMwOTBizszN NE\yVIIyOsLiaNMg M1vyWGROW09? M{fBU4lv\HWlZYOgZUBld3OnLXTldIVv\GWwdDDpcoNz\WG|ZTDpckBNSzOELVnJJINwdn[ncoPpc44h[W6mIGPRV3ROOSCmZXfyZYRifGmxbh?= M4q2ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{G1NFI5Lz5{NUexOVAzQDxxYU6=
HCT116 BAX BAK1 DKO NFTBbI5HfW6ldHnvckBCe3OjeR?= MljpN{8yOCEQvF2= NX\veW5NOTMEoHlCpC=> M1\sVWROW09? M33LfIlv\HWlZYOgZUBld3OnLXTldIVv\GWwdDDpcoNz\WG|ZTDpckBNSzOELVnJJINwdn[ncoPpc44h[W6mIGPRV3ROOSCmZXfyZYRifGmxbh?= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTdzNUCyPEc,OjV5MUWwNlg9N2F-
HCT116 NYrRfVdiTnWwY4Tpc44hSXO|YYm= M1fPRVExKM7:TR?= NYjn[ldJOTMEoHlCpC=> MWHEUXNQ NI\aU2JqdmO{ZXHz[ZMhT0[SLVzDN2IheHWwY4Th MlTCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5MUWwNlgoRjJ3N{G1NFI5RC:jPh?=
HCT116 BAX BAK1 DKO NEDq[4pHfW6ldHnvckBCe3OjeR?= NWnGWm5EOTBizszN NXi2RVJ[OTMEoHlCpC=> NILhR|JFVVOR Mny0bY5kemWjc3XzJGdHWC2OQ{PCJJB2dmO2YR?= NXfpUmc1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3NVUxOjhpPkK1O|E2ODJ6PD;hQi=>
HCT116 NHjqR3dCfXSxcHjh[5khSXO|YYm= NGXYXmkyOCEQvF2= M1f1c|EzyqCqwrC= MWHEUXNQ NXOxNoxPcW6mdXPld{BiKGOxbYDs[ZRmKGG3dH;wbIFocWNicnXzdI9ve2V? MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTdzNUCyPEc,OjV5MUWwNlg9N2F-
HCT116 BAX BAK1 DKO NULZXpc4SXW2b4DoZYd6KEG|c3H5 NYD2d4hPOTBizszN NIe3d4QyOsLiaNMg NHzmXHRFVVOR MXrpcoR2[2W|IHGgZ49ueGyndHWgZZV1d3CqYXfpZ{Bz\XOyb37z[S=> M3XjXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{G1NFI5Lz5{NUexOVAzQDxxYU6=
U937 M2DIOWFxd3C2b4Ppd{BCe3OjeR?= Mme4NE4yOjVvMjFOwG0> MWqyOEBp MWflcohidmOnczDETGEwYC1zMT3pcoR2[2WmIHHwc5B1d3Orcx?= NVG4N2xYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3NVQxOjRpPkK1O|E1ODJ2PD;hQi=>
U937  NX\UXlk1SXCxcITvd4l{KEG|c3H5 MUGwMlUh|ryP MUSyOEBp NWryVmhU\W6qYX7j[ZMh[2ynYY\h[4Uhd2ZiUFHSVEBidmRiY3HzdIF{\S1|IHHzJJdmdGxiYYOgUo95[SCuZY\lcC=> NV\QSYc6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3NVQxOjRpPkK1O|E1ODJ2PD;hQi=>
HL-60 AAA-Bcl-2 MYnBdI9xfG:|aYOgRZN{[Xl? NUPqW|dQOC13IN88US=> NWq4b3M1PDhiaB?= M4i3O2lEPTB;MD64O{DPxG4xvJzpcoR2[2W|IHPlcIwh[XCxcITvd4l{KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NYTVOmxSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3NVE1PjBpPkK1O|EyPDZyPD;hQi=>
HL-60 EEE-Bcl-2 NYK1ToNpSXCxcITvd4l{KEG|c3H5 M1vqS|AuPSEQvF2= NWfRc4p6PDhiaB?= NX3SU214UUN3ME21JO69de,:jDDpcoR2[2W|IHPlcIwh[XCxcITvd4l{KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTdzMUS2NEc,OjV5MUG0OlA9N2F-
U87 MniwSpVv[3Srb36gRZN{[Xl? NX;ueIZCPTBizszN M4nmd|I1KGh? NIfYco9z\WS3Y3XzJJRp\SCvUl7BJIV5eHKnc4Ppc44hdGW4ZXzzJI9nKE2PUD2yMEBOVVBvMUSgZY5lKEKlbD2y MlqzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4Nke2OlMoRjJ3Nk[3OlY{RC:jPh?=
K562 NI\NTWVE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NFPq[|IyNTFyIN88US=> MVK0PEBp M1HZemROW09? M3HWO2lEPTB;Mk[uO{DPxE1? NE\aWFI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW5OlU3OSd-MkW1PVY2PjF:L3G+
K562/Mcl -1-IRESBim M1z0Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUfXXm9jUUN3ME25MlMh|ryP NUjHWJRnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1N|U6ODBpPkK1OVM2QTByPD;hQi=>
K562/Bcl- 2-IRESBim MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MojFTWM2OD1yLkO1JO69VQ>? MoH5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3M{W5NFAoRjJ3NUO1PVAxRC:jPh?=
Jurkat MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTBwNk[g{txO M3XrSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUO1PVAxLz5{NUWzOVkxODxxYU6=
JurkatΔBak MkXBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoHVTWM2OD53MDFOwG0> M2PIblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUO1PVAxLz5{NUWzOVkxODxxYU6=
HL60/VCR M2\MN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHr0SW5KSzVyPkGwNEDPxE1? MnG5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3M{W5NFAoRjJ3NUO1PVAxRC:jPh?=
Kasumi-1 M{LjT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYjp[G1OUUN3ME2wMlAyKM7:TR?= MkLxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3M{W5NFAoRjJ3NUO1PVAxRC:jPh?=
Kasumi-1/ABT M3fF[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NILnZ4VKSzVyPUCuOVEh|ryP NFL4dmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUWzOVkxOCd-MkW1N|U6ODB:L3G+
THP-1 NXjFcVlDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHiyZ21KSzVyPUGuNlch|ryP MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV|NUmwNEc,OjV3M{W5NFA9N2F-
U937 NWX3XWtsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXW4blZCUUN3ME21MlI6KM7:TR?= M4flNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUO1PVAxLz5{NUWzOVkxODxxYU6=
C1498 M2LTb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{fFVWlEPTB;Nj6xN{DPxE1? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV|NUmwNEc,OjV3M{W5NFA9N2F-
RPMI 8226 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX;JR|UxRTBwMkWg{txO NEji[VI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUWzOVkxOCd-MkW1N|U6ODB:L3G+
MM.1S NE\xfnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRTBwNECg{txO MoTlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3M{W5NFAoRjJ3NUO1PVAxRC:jPh?=
NCI-H929 NFvFT2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1Xx[WlEPTB;MUWuNlEh|ryP MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV|NUmwNEc,OjV3M{W5NFA9N2F-
U266 NXHIWY1bT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFOy[mRKSzVyPUCuOlgh|ryP MnjGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3M{W5NFAoRjJ3NUO1PVAxRC:jPh?=
MCF-7 MkntR4VtdCCYaXHibYxqfHliQYPzZZk> MWi1JO69VQ>? NILKbHY1QCCq MlPjSG1UVw>? MlTw[Y5p[W6lZYOgeIhmKHOnboPpeIl3cXS7IITvJI9zKHKjZHnheIlwdg>? MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTRyOUGyOEc,OjV2MEmxNlQ9N2F-
MCF-7 MVjBdI9xfG:|aYOgRZN{[Xl? NEjjXng2KM7:TR?= MmPZOE8zPC92ODDo NH\XemZFVVOR MnW5bY5kemWjc3XzJJRp\SClbHXheoVlKFCDUmC= NEnTcJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSwPVEzPCd-MkW0NFkyOjR:L3G+
MCF-7 MmK2SpVv[3Srb36gRZN{[Xl? NHvQWIU2KM7:TR?= NH7ZUpQzPCCq NYPsW3hkTE2VTx?= MnXT[Y5p[W6lZYOgeIhmdGW4ZXygc4YhVWOuLUGg[ZhxemW|c3nvcuKh MmjpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2MEmxNlQoRjJ3NEC5NVI1RC:jPh?=
MDA-MB 231  Mmf4SpVv[3Srb36gRZN{[Xl? NWjJVol{PSEQvF2= NVTBbFdMOjRiaB?= NHLFTINFVVOR MorS[Y5p[W6lZYOgeIhmdGW4ZXygc4YhVWOuLUGg[ZhxemW|c3nvcuKh MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTRyOUGyOEc,OjV2MEmxNlQ9N2F-
ZR-75-1  MXfGeY5kfGmxbjDBd5NigQ>? NXm3[m9WPSEQvF2= MX[yOEBp M17aeWROW09? NFvIOphmdmijbnPld{B1cGWuZY\lcEBw\iCPY3ytNUBmgHC{ZYPzbY9vyqB? MkjFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2MEmxNlQoRjJ3NEC5NVI1RC:jPh?=
A549 M3rUO2NmdGxiVnnhZoltcXS7IFHzd4F6 NXLacJdvOC1{MDFOwG0> Ml\GO|IhcA>? NH[yXGlFVVOR NYfKS5Rl\GWlcnXhd4V{KHSqZTDj[YxtKHO3co\peoFtKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVzKGOxbXLpcoVlKHerdHigZZNxcXKrbh?= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN6OEe2Nkc,OjV|OEi3OlI9N2F-
H1299 M3nYTmNmdGxiVnnhZoltcXS7IFHzd4F6 M2TwS|AuOjBizszN MVO3NkBp MVHEUXNQ NYHIOoFJ\GWlcnXhd4V{KHSqZTDj[YxtKHO3co\peoFtKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVzKGOxbXLpcoVlKHerdHigZZNxcXKrbh?= M2n2elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{i4O|YzLz5{NUO4PFc3OjxxYU6=
HO-8910 NHrSSFRE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NWLWWVdUOC1{MDFOwG0> NEHHNXM4OiCq NYTxbpdPTE2VTx?= NVrwVopm\GWlcnXhd4V{KHSqZTDj[YxtKHO3co\peoFtKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVzKGOxbXLpcoVlKHerdHigZZNxcXKrbh?= MkTNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|OEi3OlIoRjJ3M{i4O|YzRC:jPh?=
HT-29 MWfD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MmPNNE0zOCEQvF2= M3HZNFczKGh? NGT5WYZFVVOR Mmj2[IVkemWjc3XzJJRp\SClZXzsJJN2en[rdnHsJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXyJINwdWKrbnXkJJdqfGhiYYPwbZJqdg>? NWGzcWM5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzPFg4PjJpPkK1N|g5PzZ{PD;hQi=>
HCT-116 MW\D[YxtKF[rYXLpcIl1gSCDc4PhfS=> NF;BN4UxNTJyIN88US=> NInjPZk4OiCq MX\EUXNQ M2jrc4Rm[3KnYYPld{B1cGViY3XscEB{fXK4aY\hcEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldkBkd22kaX7l[EB4cXSqIHHzdIlzcW5? M3:1NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{i4O|YzLz5{NUO4PFc3OjxxYU6=
A549 M{XGOmFxd3C2b4Ppd{BCe3OjeR?= NGP6NJczOCEQvF2= MY[0PEBp MWPEUXNQ MXTpcoR2[2W|IHHwc5B1d3OrczDzbYdvcW[rY3HueIx6KGOxbXLpcoVlKHerdHigZZNxcXKrbh?= M3;UcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{i4O|YzLz5{NUO4PFc3OjxxYU6=
H1299 Mn\yRZBweHSxc3nzJGF{e2G7 M2\mPFIxKM7:TR?= NH:wZXY1QCCq M2G0WmROW09? NF3ZdFlqdmS3Y3XzJIFxd3C2b4Ppd{B{cWewaX\pZ4FvfGy7IHPvcYJqdmWmIIfpeIgh[XOyaYLpci=> NYrH[|JJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzPFg4PjJpPkK1N|g5PzZ{PD;hQi=>
Sc-1 MWfD[YxtKF[rYXLpcIl1gSCDc4PhfS=> Ml\FNE4xODBzLUGg{txO NXfodoFpQTZiaB?= Ml;s[IVkemWjc3XzJJRp\SClZXzsJJZq[WKrbHn0fUBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MlrzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|N{O1NFgoRjJ3M{ezOVA5RC:jPh?=
OcI-LY18 MkGxR4VtdCCYaXHibYxqfHliQYPzZZk> M2SxdlAvODByMT2xJO69VQ>? NYHCOpdRQTZiaB?= MVHk[YNz\WG|ZYOgeIhmKGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NXzTR|ZQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzO|M2ODhpPkK1N|c{PTB6PD;hQi=>
RL  MXfD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NInqTZExNjByMEGtNUDPxE1? NVn5T5RRQTZiaB?= M3TsUoRm[3KnYYPld{B1cGViY3XscEB3cWGkaXzpeJkhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NXfEO3ZSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzO|M2ODhpPkK1N|c{PTB6PD;hQi=>
RKO MYfD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MXSwMVExKM7:TR?= MXGyOQKBkWkEoB?= MUTEUXNQ MX3JR|UxRuLCiUK15qCKyrWP MmXPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|MESzPFMoRjJ3M{C0N|g{RC:jPh?=
Caco-2 MWHD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NI\PNncxNTFyIN88US=> M{DGe|I16oDLaNMg NEixR45FVVOR MkTQTWM2OD1zOT635qCKyrWP MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTNyNEO4N{c,OjV|MESzPFM9N2F-
DLD1 M3jtWWNmdGxiVnnhZoltcXS7IFHzd4F6 NF7Ib4oxNTFyIN88US=> NVy1RWhlOjUkgJnoxsA> NUXX[oVLTE2VTx?= MXLJR|UxRTF6Lke45qCKyrWP MoLFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|MESzPFMoRjJ3M{C0N|g{RC:jPh?=
LS411N MlX1R4VtdCCYaXHibYxqfHliQYPzZZk> NWDTXpJ7OC1zMDFOwG0> NFLSWHYzPOLCiXlCpC=> MmrhSG1UVw>? M{HBVGlEPTB;MUGuOFfjiIoEtV2= M17zNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{C0N|g{Lz5{NUOwOFM5OzxxYU6=
SW620 MnG2R4VtdCCYaXHibYxqfHliQYPzZZk> M1fYR|AuOTBizszN NXnv[mdpOjUkgJnoxsA> NFL2eWtFVVOR MlvFTWM2OD1zMj6yOQKBkcL3TR?= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTNyNEO4N{c,OjV|MESzPFM9N2F-
HCT116 NWjOUFJGS2WubDDWbYFjcWyrdImgRZN{[Xl? MV6wMVExKM7:TR?= NVLlcXRUOjUkgJnoxsA> M4ry[GROW09? Ml64TWM2OD1{MD60PgKBkcL3TR?= Mn;BQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|MESzPFMoRjJ3M{C0N|g{RC:jPh?=
HaCaT M3v4TGNmdGxiVnnhZoltcXS7IFHzd4F6 M4H6VVAvOS9zL{GwJO69VQ>? NIXIPFMzPCCq MV\EUXNQ NIP6Sphl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NXnpT5pZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyNVA4QTVpPkK1NlExPzl3PD;hQi=>
A5-RT3 M{D0N2NmdGxiVnnhZoltcXS7IFHzd4F6 MWSwMlEwOS9zMDFOwG0> NH;QfnMzPCCq MlLGSG1UVw>? MmXO[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ MlTaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{MUC3PVUoRjJ3MkGwO|k2RC:jPh?=
HaCaT MVLGeY5kfGmxbjDBd5NigQ>? MUKxNQKBkc7:TR?= MV:yOE81QCCq NFzzfWlFVVOR M3f1SIlv\HWlZYOgUW1RKGGwZDDEUmEh\nKjZ33lcpRifGmxbh?= NH\oPHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUKxNFc6PSd-MkWyNVA4QTV:L3G+
A5-RT3 MWHGeY5kfGmxbjDBd5NigQ>? NFnRd5AyOOLCid88US=> NID6Z5QzPC92ODDo MnnjSG1UVw>? NYD6VpJocW6mdXPld{BOVVBiYX7kJGRPSSCocnHncYVvfGG2aX;u NWXWZZVXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyNVA4QTVpPkK1NlExPzl3PD;hQi=>
A5-RT3 NGrxZoZHfW6ldHnvckBCe3OjeR?= MojrOUDPxE1? MVm2JIg> Mk\uSG1UVw>? M2DmZolv\HWlZYOgeIhmKHKnbHXhd4Uhd2ZibXn0c4Npd26mcnnhcEBxem:2ZXnud{BidmRicnXkeYNmeyClbH;uc4dmdmmlIIP1dpZqfmGuIHnuJIEh[2G|cHHz[U1qdmSncHXu[IVvfCCvYX7u[ZI> Mm\PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{MUC3PVUoRjJ3MkGwO|k2RC:jPh?=
U266 MWfGeY5kfGmxbjDBd5NigQ>? M1;ufFUxOC95NUCgcm0> MluwNlQwPDhiaB?= MUjEUXNQ MV\kc5dvemWpdXzheIV{KEKrbTygdJJqdmOrcHHscJkhfGinIFXMJIl{d2[xcn2= MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJyOEi4PEc,OjV{MEi4PFg9N2F-
RPMI8226 MkXCSpVv[3Srb36gRZN{[Xl? NUDRXZBFPTByL{e1NEBvVQ>? MkPNNlQwPDhiaB?= NGixVIhFVVOR M2nwZYRwf26{ZXf1cIF1\XNiQnntMEBxemmwY3nwZYxtgSC2aHWgSWwhcXOxZn;ycS=> M1\v[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MkC4PFg5Lz5{NUKwPFg5QDxxYU6=
MM.1S NWPtXmhWTnWwY4Tpc44hSXO|YYm= NGjVSY82ODBxN{WwJI5O NFzEdmkzPC92ODDo Mon6SG1UVw>? M3u0foRwf26{ZXf1cIF1\XNiQnntMEBxemmwY3nwZYxtgSC2aHWgSWwhcXOxZn;ycS=> M4HZVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MkC4PFg5Lz5{NUKwPFg5QDxxYU6=
Clone A M4j3TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYGwMlLjiJN4MDFOwG0> M2XG[|czKGh? MXTEUXNQ Mm\yTWM2OD15LkWg{txO MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJyOEi4Nkc,OjV{MEi4PFI9N2F-
CX-1 M3;QRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\UOXh1OC5{4pETOlAh|ryP NWDCOWx4PzJiaB?= NGLVeWVFVVOR NHWw[IFKSzVyPUGuPEDPxE1? NHzvdYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUKwPFg5Oid-MkWyNFg5QDJ:L3G+
LS174T MlTUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjXboRSOC5{4pETOlAh|ryP MVO3NkBp M{D6XWROW09? NEW0fVVKSzVyPUG4MlMh|ryP NUPTSJhoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyNFg5QDJpPkK1NlA5QDh{PD;hQi=>
HT29 MmHhRZBweHSxc3nzJGF{e2G7 MUexM|UwOTBizszN MlrMOFghcA>? M{DGS4NifXOnczDj[YxtKGSnYYToJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NIrqfpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG5NlE5QCd-MkWxPVIyQDh:L3G+
SW480 M{Ht[mFxd3C2b4Ppd{BCe3OjeR?= NVTscJB5OS93L{GwJO69VQ>? MmnnOFghcA>? NIH6bmRk[XW|ZYOgZ4VtdCCmZXH0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NHPDbo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG5NlE5QCd-MkWxPVIyQDh:L3G+
Colo205 M2fTfGFxd3C2b4Ppd{BCe3OjeR?= NX;mdopsOS93L{GwJO69VQ>? NVnUbFhtPDhiaB?= MVHjZZV{\XNiY3XscEBl\WG2aDDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= M{i3clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUmyNVg5Lz5{NUG5NlE5QDxxYU6=
Caco2 NUi0VXQySXCxcITvd4l{KEG|c3H5 M4XBZlEwPS9zMDFOwG0> MlizOFghcA>? MlS2Z4F2e2W|IHPlcIwh\GWjdHigbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= M{\ufFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUmyNVg5Lz5{NUG5NlE5QDxxYU6=
PCI-13 MnXvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MW[3NkBp NWno[mg3TE2VTx?= M4rlNGdKPTB;MUWgxtEhOS56IN88US=> MkDwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzM{mzPFcoRjJ3MUO5N|g4RC:jPh?=
PCI-15B M3TVRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEHzVoQ4OiCq MYLEUXNQ NVe3OnJ1T0l3ME2xNUDDuSB2LkWg{txO MnjlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzM{mzPFcoRjJ3MUO5N|g4RC:jPh?=
UM-SCC22B M3Xxcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnjxO|IhcA>? NE\wRohFVVOR NXHveZVlT0l3ME2xPUDDuSB{Lkmg{txO MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF|OUO4O{c,OjVzM{mzPFc9N2F-
UM-SCC47 NXPNenRmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVnyb25rPzJiaB?= MX3EUXNQ MoPkS2k2OD1zOTFCtUAyOi5|IN88US=> NEHzUYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGzPVM5Pyd-MkWxN|k{QDd:L3G+
93-VU-147T NHL2VZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NInMbow4OiCq MWHEUXNQ MV;HTVUxRTRwMzFCtUA{NjVizszN NHHpTok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGzPVM5Pyd-MkWxN|k{QDd:L3G+
UD-SCC2 M1jKVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFzHb2U4OiCq M13Db2ROW09? Mo\vS2k2OD1{ODFCtUAzNjlizszN NHq2bGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGzPVM5Pyd-MkWxN|k{QDd:L3G+
UPCI:SCC90 NGjkZ5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEfsdpY4OiCq NWjUeGZITE2VTx?= NGLvPVFIUTVyPU[uOkDDuSBzLkWg{txO NVfRSpd3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxN|k{QDdpPkK1NVM6Ozh5PD;hQi=>
RPMI-8226  NWHlfIRmS2WubDDWbYFjcWyrdImgRZN{[Xl? MX2xNlUwOjVyL{WwNEBvVQ>? MWK0PIjDqA>? MUjEUXNQ M3nYOoRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MlHTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyMEiyNFIoRjJ3MEC4NlAzRC:jPh?=
OPM-2  MnvHR4VtdCCYaXHibYxqfHliQYPzZZk> MXKxNlUwOjVyL{WwNEBvVQ>? MYm0PIjDqA>? NUTudXE2TE2VTx?= MkDS[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ MlmzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyMEiyNFIoRjJ3MEC4NlAzRC:jPh?=
RPMI-8226  M4CyS2Fxd3C2b4Ppd{BCe3OjeR?= NGfrW4gyOjVxMkWwM|UxOCCwTR?= NVzHeG02PDiqwrC= Mlr4SG1UVw>? Mn21bY5lfWOnczDj[YxtKGGyb4D0c5NqeyCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTByOEKwNkc,OjVyMEiyNFI9N2F-
OPM-2  MUfBdI9xfG:|aYOgRZN{[Xl? NGCw[4wyOjVxMkWwM|UxOCCwTR?= MUW0PIjDqA>? NHPKbJJFVVOR NHSzVpNqdmS3Y3XzJINmdGxiYYDvdJRwe2m|IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ M1frW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEC4NlAzLz5{NUCwPFIxOjxxYU6=
COG-LL-319 MUjGeY5kfGmxbjDBd5NigQ>? MlrmNVAxKG6P NX35OmJsOS9|L{[gbC=> NXuxPG5DTE2VTx?= M1KyeYlv\HWlZYOgZ4F{eGG|ZT3k[ZBmdmSnboSgUYNtNTFiY3zlZZZi\2V? MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl3MUS3Nkc,OjR7NUG0O|I9N2F-
RS4;11 NVfjVot1TnWwY4Tpc44hSXO|YYm= NUO2bGFVOTByIH7N M4HUTVEwOy94IHi= MX;EUXNQ M{i5Z4lv\HWlZYOgZ4F{eGG|ZT3k[ZBmdmSnboSgUYNtNTFiY3zlZZZi\2V? NVfNZYlVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OVE1PzJpPkK0PVUyPDd{PD;hQi=>
FL5.12 M3SzZWZ2dmO2aX;uJIF{e2G7 MWPS[ZZmenOjbDDv[kBkgXSxa3nu[UB4cXSqZILhe4FtKHC{b4TlZ5Rqd25iaX6gTWw{KGSncHXu[IVvfCCEY3yyJI93\XKneIDy[ZN{cW6pIH3veZNmKE[ONT6xNkBk\WyuczDpckBxemW|ZX7j[UBw\iCkb4\pcoUh\2WuYYTpckwhTUN3MDC9JFAvODB6IN88UU4> MmnkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd{NU[4N|QoRjF5MkW2PFM1RC:jPh?=
DoHH2 NFXY[mlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M17QZWlvcGmkaYTpc44hd2ZiY3XscEBoem:5dHigbY4hcHWvYX6gSI9JUDJiY3XscJMhd3[ncnX4dJJme3OrbnegRoNtOiCrbjDwdoV{\W6lZTDv[kA{LSCIQmOsJGVEPTBiPTCwMlAxQDNizszNMi=> Mki5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd{NU[4N|QoRjF5MkW2PFM1RC:jPh?=
RS11380 MW\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NELGN4NKdmirYnn0bY9vKG:oIHPlcIwh\3Kxd4ToJIlvKGi3bXHuJHJUOTF|OECgZ4VtdHNib4\ldoV5eHKnc4PpcochSmOuMjDpckBxemW|ZX7j[UBw\iB|JTDGRnMtKEWFNUCgQUAxNjBzNDFOwG0v NY\UOXR5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUeyOVY5OzRpPkG3NlU3QDN2PD;hQi=>
FL5.12 MoTSSpVv[3Srb36gZZN{[Xl? NVvkPJF{WmW4ZYLzZYwhd2ZiY4n0c4tqdmVid3n0bIRz[XejbDDwdo91\WO2aX;uJIlvKEmOMzDk[ZBmdmSnboSgRoNtNXiOIH;2[ZJmgHC{ZYPzbY5oKG2xdYPlJGZNPS5zMjDj[YxteyCrbjDwdoV{\W6lZTDv[kBjd3[rbnWg[4Vt[XSrbjygSWM2OCB;IECuNFMh|ryPLh?= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzJ3NkizOEc,OTd{NU[4N|Q9N2F-
FL5.12 MlfzSpVv[3Srb36gZZN{[Xl? NGqzUoFT\X[ncoPhcEBw\iCleYTvb4lv\SC5aYTo[JJif2GuIIDyc5Rm[3Srb36gbY4hUUx|IHTldIVv\GWwdDDCZ4wzKG:4ZYLlfJBz\XO|aX7nJI1wfXOnIF\MOU4yOiClZXzsd{BqdiCycnXz[Y5k\SCxZjCzKUBHSlNuIFXDOVAhRSByLkC1JO69VS5? M2LEdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MkW2PFM1Lz5zN{K1Olg{PDxxYU6=
DoHH2 NYXTW2dHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHvz[WZKdmirYnn0bY9vKG:oIHPlcIwh\3Kxd4ToJIlvKGi3bXHuJGRwUEh{IHPlcIx{KG:4ZYLlfJBz\XO|aX7nJGJkdDJiaX6gdJJme2WwY3Wgc4YhOTBnIFjTMEBGSzVyIE2gNE4yOyEQvF2u NF34fnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{K1Olg{PCd-MUeyOVY5OzR:L3G+
RS11380 Ml62S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWL4PFAzUW6qaXLpeIlwdiCxZjDj[YxtKGe{b4f0bEBqdiCqdX3hckBTWzFzM{iwJINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEKlbEKgbY4heHKnc3XuZ4Uhd2ZiMUClJGhUNCCHQ{WwJF0hOC5zNTFOwG0v MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzJ3NkizOEc,OTd{NU[4N|Q9N2F-
SUDHL4 NHrs[mRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NV;uPW1rUW6qaXLpeIlwdiCxZjDj[YxtKGe{b4f0bEBqdiCqdX3hckBUXUSKTESgZ4VtdHNib4\ldoV5eHKnc4PpcochSmOuMjDpckBxemW|ZX7j[UBw\iB|JTDGRnMtKEWFNUCgQUAxNjJ{IN88UU4> NIDXeYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{K1Olg{PCd-MUeyOVY5OzR:L3G+
FL5.12 NGnHWW1HfW6ldHnvckBie3OjeR?= NF;VSHNT\X[ncoPhcEBw\iCleYTvb4lv\SC5aYTo[JJif2GuIIDyc5Rm[3Srb36gbY4hUUx|IHTldIVv\GWwdDDCZ4wugExib4\ldoV5eHKnc4PpcochdW:3c3WgSmw2NjF{IHPlcIx{KGmwIIDy[ZNmdmOnIH;mJFMmKE[EUzygSWM2OCB;IECuNlIh|ryPLh?= MnflQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd{NU[4N|QoRjF5MkW2PFM1RC:jPh?=
SUDHL4 MUXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWHJcohq[mm2aX;uJI9nKGOnbHyg[5Jwf3SqIHnuJIh2dWGwIGPVSGhNPCClZXzsd{BwfmW{ZYjwdoV{e2mwZzDCZ4wzKGmwIIDy[ZNmdmOnIH;mJFExLSCKUzygSWM2OCB;IECuPFUh|ryPLh?= NHPhfHc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{K1Olg{PCd-MUeyOVY5OzR:L3G+
FL5.12 NUS0XG9JS3m2b4TvfIlkcXS7IHHzd4F6 M3y2VVI1KGi{cx?= MmrjR5l1d3SxeHnjbZR6KGGpYXnud5QhUUx|LXTldIVv\GWwdDDtc5V{\SCITEWuNVIh[2WubIOgc5ZmemW6cILld5NqdmdiaIXtZY4hSmOuMjDhd5Nme3OnZDDhd{Bk\WyuII\pZYJqdGm2eTDh[pRmeiB{NDDodpMh[nliTWTTJIF{e2G7IHnuJIFje2WwY3Wgc4Yhe2W{dX2sJGVEPTBiPTCwMlAxPzdizszNMi=> MlrnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh6NEG4PFIoRjF6OESxPFgzRC:jPh?=
FL5.12 MXvDfZRwfG:6aXPpeJkh[XO|YYm= NIX2OpozPCCqcoO= MXLDfZRwfG:6aXPpeJkh[WejaX7zeEBKVDNvZHXw[Y5l\W62IH3veZNmKE[ONT6xNkBk\WyuczDveoVz\XiycnXzd4lv\yCqdX3hckBD[2xvWFygZZN{\XO|ZXSgZZMh[2WubDD2bYFjcWyrdImgZYZ1\XJiMkSgbJJ{KGK7IF3UV{Bie3OjeTDpckBi[nOnbnPlJI9nKHOncoXtMEBGSzVyIE2gNE4xOyEQvF2u MlrHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh6NEG4PFIoRjF6OESxPFgzRC:jPh?=
NCI-H146 MnzZR5l1d3SxeHnjbZR6KGG|c3H5 MlnsOFghcHK| MVvDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDOR2kuUDF2NjDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOgbY4heHKnc3XuZ4Uhd2ZiMUClJIh2dWGwIIPldpVuNCCHQ{WwJF0hOC5yOEeg{txONg>? NX35[HF4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi4OFE5QDJpPkG4PFQyQDh{PD;hQi=>
CLL M1f6SGFxd3C2b4Ppd{Bie3OjeR?= MU\JcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKEOOTDDj[YxteyxiRVO1NEA:KDBwMEC0OUDPxE1w MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODl{NUSzN{c,OjB7MkW0N|M9N2F-
MEF MnfXR5l1d3SxeHnjbZR6KGG|c3H5 M3vKZVI1KGi{cx?= NHzrVFJEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBu[2xvMTDk[YZq[2mnboSgUWVHKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDD[YxtKHSrdHXyJIdtdyCjc4PhfUBqdiCycnXz[Y5k\SCxZjCxKUB{\XK3bTygSWM2OCB;IECuNFAzODNizszNMi=> Ml7hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|Nk[yPVUoRjJzM{[2Nlk2RC:jPh?=
MEF MV7DfZRwfG:6aXPpeJkh[XO|YYm= MUCyOEBpenN? MmPvR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgcYNtNTFiZHXmbYNq\W62IF3FSkBk\WyuczDh[pRmeiB{NDDodpMh[nliQ3XscEB1cXSncjDncI8h[XO|YYmgbY4heHKnc3XuZ4Uhd2ZiMUClJJNmenWvLDDFR|UxKD1iMD6wOVEh|ryPLh?= NHXCUXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUO2OlI6PSd-MkGzOlYzQTV:L3G+
MEF MlXMR5l1d3SxeHnjbZR6KGG|c3H5 M2LFOlI1KGi{cx?= NYXob2E5S3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhdWOuLUGg[IVncWOrZX70JG1GTiClZXzsd{Bi\nSncjCyOEBpenNiYomgR4VtdCC2aYTldkBodG9iYYPzZZkhcW5icILld4Vv[2Vib3[gNVAmKG[ndHHsJIJwfmmwZTDz[ZJ2dSxiRVO1NEA:KDBwMEWxJO69VS5? MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN4NkK5OUc,OjF|Nk[yPVU9N2F-
RS4:11 MWLBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NGPIWGdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGLTOFoyOSClZXzsd{BqdiCycnXz[Y5k\SCxZjCxNEUhcHWvYX6gd4VzfW1uIFXDOVAhRSByLkCyOEDPxE1w MlrqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7Mk[yOFcoRjJ6OUK2NlQ4RC:jPh?=
MOLT4 NILyXHpCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NY\nV4VrSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNU2xVPCClZXzsd{BqdiCycnXz[Y5k\SCxZjCxNEUhcHWvYX6gd4VzfW1uIFXDOVAhRSByLk[yNkDPxE1w Ml6zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7Mk[yOFcoRjJ6OUK2NlQ4RC:jPh?=
Jurkat MmLqR5l1d3SxeHnjbZR6KGG|c3H5 M{jYc|Q5KGi{cx?= NX;E[Y9ES3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUnW{a3H0JINmdGy|IHHmeIVzKDR6IHjyd{BjgSClZXzsJJRqfGW{LXLseYUh[XO|YYmsJGlEPTBiPTCxMlM5KM7:TT6= NEXXSmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUe0N|g2QCd-MUm3OFM5PTh:L3G+
HCT116 M4j5VmN6fG:2b4jpZ4l1gSCjc4PhfS=> MWq0PEBpenN? M{m1fGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDFzNjDj[YxteyCjZoTldkA1QCCqcoOgZpkh[2WubDD0bZRmei2kbIXlJIF{e2G7LDDJR|UxKD1iND6wOkDPxE1w M1vBb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{SzPFU5Lz5zOUe0N|g2QDxxYU6=
SU-8686 M{fQUmN6fG:2b4jpZ4l1gSCjc4PhfS=> Mnv3OFghcHK| MoDlR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV3UuQDZ6NjDj[YxteyCjZoTldkA1QCCqcoOgZpkh[2WubDD0bZRmei2kbIXlJIF{e2G7LDDJR|UxKD1iND6yOEDPxE1w MkHyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl5NEO4OVgoRjF7N{SzPFU5RC:jPh?=
H460 NHrL[|JEgXSxdH;4bYNqfHliYYPzZZk> MlnvOFghcHK| NF\XZ3pEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJPDZyIHPlcIx{KGGodHXyJFQ5KGi{czDifUBk\WyuIITpeIVzNWKudXWgZZN{[XluIFnDOVAhRSB6LkCzJO69VS5? M2jwc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{SzPFU5Lz5zOUe0N|g2QDxxYU6=
Hepa-1c1c7 NY[0Z|ZnS3m2b4TvfIlkcXS7IHHzd4F6 NUntPWJNPDhiaILz MXLDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDI[ZBiNTGlMXO3JINmdGy|IHHmeIVzKDR6IHjyd{BjgSClZXzsJJRqfGW{LXLseYUh[XO|YYmsJGlEPTBiPTC4MlY5KM7:TT6= NFrYcGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUe0N|g2QCd-MUm3OFM5PTh:L3G+
MCF7 MmS3R5l1d3SxeHnjbZR6KGG|c3H5 NFzsNYw1QCCqcoO= MlO1R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjC0PEBpenNiYomgZ4VtdCC2aYTldk1jdHWnIHHzd4F6NCCLQ{WwJF0hOjFwMk[g{txONg>? M{S0WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{SzPFU5Lz5zOUe0N|g2QDxxYU6=
DU145 NVzh[HpIS3m2b4TvfIlkcXS7IHHzd4F6 M{D4clQ5KGi{cx?= M3n4VWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGRWOTR3IHPlcIx{KGGodHXyJFQ5KGi{czDifUBk\WyuIITpeIVzNWKudXWgZZN{[XluIFnDOVAhRSB{Nz62JO69VS5? MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTd2M{i1PEc,OTl5NEO4OVg9N2F-
HCT116 NEXiRo5EgXSxdH;4bYNqfHliYYPzZZk> NIK3NnQ4OiCqcoO= Mo\GR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVOTF4IHPlcIx{KGW6cILld5NqdmdiQnPsMZhNNCCEY3ytNkBidmRiTXPsMVEh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFQ4NjdizszNMi=> Ml3UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzN{K3NFEoRjJ{MUeyO|AyRC:jPh?=
BL21 (DE3) MVLGeY5kfGmxbjDhd5NigQ>? NHLFRWwzKGi{cx?= NFzX[lhDcW6maX7nJIFn\mmwaYT5JJRwKE5vdHXycYlvfXNiNmigTIl{NXSjZ3fl[EBpfW2jbjDCZ4wzKGW6cILld5Nm\CCrbjDFd4Np\XKrY3jpZUBkd2yrIFLMNlEhMESHMzmgZ4VtdHNiYX\0[ZIhOiCqcoOgZpkh\my3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeTygTWM2OCB;IECuNFAzKM7:TT6= MkmxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2NEi5PFgoRjJ{NES4PVg5RC:jPh?=
BL21 (DE3) MYfGeY5kfGmxbjDhd5NigQ>? MoPMNkBpenN? M1LDO2JqdmSrbnegZYZncW6rdImgeI8hVi22ZYLtbY52eyB6WDDIbZMufGGpZ3XkJIh2dWGwIFLjcE15VCCneIDy[ZN{\WRiaX6gSZNkcGW{aXPobYEh[2:uaTDCUFIyKCiGRUOpJINmdGy|IHHmeIVzKDJiaILzJIJ6KG[udX;y[ZNk\W6lZTDwc4xiemm8YYTpc44h[XO|YYmsJGlEPTBiPTCwMlAxPiEQvF2u M3OzZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NES4PVg5Lz5{MkS0PFk5QDxxYU6=
NCI-H146 NGT0TYJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoOxOEBl[Xm| M2\wVWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUS2JINmdGy|IHHmeIVzKDRiZHH5d{BjgSCZU2S4JIF{e2G7LDDJR|UxKD1iMD6wPVch|ryPLh?= NFjBfnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkS0PFk5QCd-MkK0OFg6QDh:L3G+
NCI-H1417 MnnrS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUe5TWR3PCCmYYnz MoTjS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gUmNKNUhzNEG3JINmdGy|IHHmeIVzKDRiZHH5d{BjgSCZU2S4JIF{e2G7LDDJR|UxKD1iMD6xN{DPxE1w NU[wdGtqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0OFg6QDhpPkKyOFQ5QTh6PD;hQi=>
CCRF-CEM NFf4PJNEgXSxdH;4bYNqfHliYYPzZZk> NWrtPJNqPDhiaILz MXXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDDR3JHNUOHTTDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOgZpkh[2WubITpeIVzNWKudXWgZZN{[XluIFnDOVAhRSByLke0JO69VS5? MnvnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3OEK5PVEoRjJ{NUiyPVkyRC:jPh?=
HL60 NUPofFlES3m2b4TvfIlkcXS7IHHzd4F6 MYO0PEBpenN? M4\tRWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNPjBiY3XscJMh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK|IHL5JINmdGy2aYTldk1jdHWnIHHzd4F6NCCLQ{WwJF0hOC55NjFOwG0v NGHQWpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW4Nlk6OSd-MkK1PFI6QTF:L3G+
K562 NYK1VY04S3m2b4TvfIlkcXS7IHHzd4F6 MoDxOFghcHK| NGj4VWJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMPTZ{IHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBk\WyudHn0[ZIu[my3ZTDhd5NigSxiSVO1NEA:KDN2Lkeg{txONg>? M{HJN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUiyPVkyLz5{MkW4Nlk6OTxxYU6=
NCI-H146 NF:zd49CdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MnTIOEBl[Xm| MYTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE6FST3INVQ3KGOnbHzzJIFnfGW{IESg[IF6eyCkeTDXV3Q5KGG|c3H5MEBKSzVyIE2gNE4xOzdizszNMi=> NWTYdXhJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3OFc2QThpPkKyO|Q4PTl6PD;hQi=>
NCI-H1963 NWj5blhJSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NUO2T3g5PCCmYYnz NELWOppCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF7DTU1JOTl4MzDj[YxteyCjZoTldkA1KGSjeYOgZpkhX1OWODDhd5NigSxiSVO1NEA:KDBwMEW5JO69VS5? NGG4NIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0O|U6QCd-MkK3OFc2QTh:L3G+
NCI-H1417 NGHRR|BCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NUHr[GtjPCCmYYnz MUjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE6FST3INVQyPyClZXzsd{Bi\nSncjC0JIRigXNiYomgW3NVQCCjc4PhfUwhUUN3MDC9JFAvPDF{IN88UU4> NWmyZ2J6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3OFc2QThpPkKyO|Q4PTl6PD;hQi=>
RS4:11 MWTBdI9xfG:|aYOgZZN{[Xl? M{HNXVQ5KGi{cx?= NX;GRo5ZUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBD[2x{IHTldIVv\GWwdDDoeY1idiCUU{S6NVEh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IFHucoV5cW5iVjDzeIFqdmmwZzDiZZNm\CCobH;3JIN6fG:vZYTyfUwhUUN3MDC9JFAvOjdizszNMi=> Mlr2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|MUSwOVQoRjJ|M{G0NFU1RC:jPh?=
K562 NFXBUmlCeG:ydH;zbZMh[XO|YYm= NVi4eXNLPDhiaILz MkDNTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDNZ4wyKGSncHXu[IVvfCCqdX3hckBMPTZ{IHPlcIx{KGGodHXyJFQ5KGi{czDifUBCdm6neHnuJHYhe3SjaX7pcoch[mG|ZXSg[oxwfyCleYTvcYV1enluIFnDOVAhRSBzNj60JO69VS5? NIDiUYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{OxOFA2PCd-MkOzNVQxPTR:L3G+
NCI-H1963 NYH4d|BsS3m2b4TvfIlkcXS7IHHzd4F6 MUW0JIRigXN? MkDBR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUmNKNUhzOU[zJINmdGy|IHHzd4V{e2WmIHHzJIdzd3e2aDDpcohq[mm2aX;uJIFnfGW{IESg[IF6eyCkeTDXV3Qh[XO|YYmsJGlEPTBiPTCwMlA2PCEQvF2u MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2OEK5PEc,OjN2NEiyPVg9N2F-
NCI-H187 M4\h[GN6fG:2b4jpZ4l1gSCjc4PhfS=> NX6yZWg3PCCmYYnz NGTWW2hEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBPS0lvSEG4O{Bk\WyuczDhd5Nme3OnZDDhd{Boem:5dHigbY5pcWKrdHnvckBi\nSncjC0JIRigXNiYomgW3NVKGG|c3H5MEBKSzVyIE2gNE4yOzd5IN88UU4> Ml6yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NEiyPVgoRjJ|NES4Nlk5RC:jPh?=
NCI-H1417 MVXDfZRwfG:6aXPpeJkh[XO|YYm= MUm0JIRigXN? MXnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDOR2kuUDF2MUegZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiNDDkZZl{KGK7IGfTWEBie3OjeTygTWM2OCB;IECuNVc{PCEQvF2u MkjEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NEiyPVgoRjJ|NES4Nlk5RC:jPh?=
HL60 M3zrXWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnPDO|IhcHK| NVXUNJVFT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hUEx4MDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC57NzFOwG0v NFjPe289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{exNlk{QSd-Mke3NVI6Ozl:L3G+
MCF7 MVfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWO3NkBpenN? NXfmV2VrT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVUOINzDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOjVwM{Og{txONg>? NGjuToE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{exNlk{QSd-Mke3NVI6Ozl:L3G+
U266 MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlXzO|IhcHK| NF;TSllIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBWOjZ4IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAzPy5|NTFOwG0v NWLj[optRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke3NVI6OzlpPkK3O|EzQTN7PD;hQi=>
SKOV3 NEjoS|FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnHiO|IhcHK| NHW0SnZIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBUU0:YMzDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hPDZwNUmg{txONg>? NGi1T2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{exNlk{QSd-Mke3NVI6Ozl:L3G+
RS4:11 NFfhfpVEgXSxdH;4bYNqfHliYYPzZZk> NHTlbmUzPCCqcoO= MWPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDSV|Q7OTFiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDJ2IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkOzJO69VS5? NWHFWVF1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0OVMyOzVpPkK5OFU{OTN3PD;hQi=>
Remb1 M4fpZ2N6fG:2b4jpZ4l1gSCjc4PhfS=> Mm\0NlQhcHK| M1foR2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHJmdWJzIHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB{NDDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMT60JO69VS5? NV:zVW5JRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0OVMyOzVpPkK5OFU{OTN3PD;hQi=>
BL21 NVOxc2gxTnWwY4Tpc44h[XO|YYm= NXTVfnlxOTBidV2= MlTlN{BpenN? M{\M[2Rqe3CuYXPlcYVvfCCxZjDCU2RKWFlvQnHrJINwdmq3Z3H0[YQheGWydHnk[UBnem:vIFfTWE11[WepZXSgbJVu[W5iQnPsMVIudGmtZTDwdo91\WmwIEGgS|E6PkFibYX0ZY51KGW6cILld5Nm\CCrbjDFd4Np\XKrY3jpZUBkd2yrIFLMNlEh[2WubIOgZZQhOTBidV2gZYZ1\XJiMzDodpMh[nliZnz1c5Jme2OnbnPlJJBwdGG{aYrheIlwdiClb33w[ZRqfGmxbjDhd5NigSxiS3mgQUAxNjByMTFOwG0v MnWxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6MEe1NVIoRjJzOEC3OVEzRC:jPh?=
BL21 MlLKSpVv[3Srb36gZZN{[Xl? MmPTNVAhfU1? M3\oelMhcHK| NHPBTFhFcXOybHHj[Y1mdnRib3[gRm9FUVC\LVLhb{Bkd26sdXfheIVlKHCncITp[IUh\nKxbTDHV3QufGGpZ3XkJIh2dWGwIFLjcE0zNWyra3WgdJJwfGWrbjCxJGE6O1ZibYX0ZY51KGW6cILld5Nm\CCrbjDFd4Np\XKrY3jpZUBkd2yrIFLMNlEh[2WubIOgZZQhOTBidV2gZYZ1\XJiMzDodpMh[nliZnz1c5Jme2OnbnPlJJBwdGG{aYrheIlwdiClb33w[ZRqfGmxbjDhd5NigSxiS3mgQUAxNjByMUSg{txONg>? MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOThyN{WxNkc,OjF6MEe1NVI9N2F-
BL21 M2Pne2Z2dmO2aX;uJIF{e2G7 NXjONIY5OTBidV2= NY\x[pFkOyCqcoO= NILDZ4pFcXOybHHj[Y1mdnRib3[gRm9FUVC\LVLhb{Bkd26sdXfheIVlKHCncITp[IUh\nKxbTDHV3QufGGpZ3XkJIh2dWGwIFLjcE0zNWyra3WgdJJwfGWrbjCxJHkyQTWIIH31eIFvfCCneIDy[ZN{\WRiaX6gSZNkcGW{aXPobYEh[2:uaTDCUFIyKGOnbHzzJIF1KDFyIIXNJIFnfGW{IEOgbJJ{KGK7IH\seY9z\XOlZX7j[UBxd2yjcnn6ZZRqd25iY3;tdIV1cXSrb36gZZN{[XluIFvpJF0hOC5yMEG1JO69VS5? NIridHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUiwO|UyOid-MkG4NFc2OTJ:L3G+
BL21 NVPzcmQ1TnWwY4Tpc44h[XO|YYm= M2\2NVExKHWP NUnvXnlJOyCqcoO= M3\pZmRqe3CuYXPlcYVvfCCxZjDCU2RKWFlvQnHrJINwdmq3Z3H0[YQheGWydHnk[UBnem:vIFfTWE11[WepZXSgbJVu[W5iV3ns[EB1gXCnIFLjcE0zNWyra3WgdJJwfGWrbjCxJIV5eHKnc4Pl[EBqdiCHc3Po[ZJq[2irYTDjc4xqKEKOMkGgZ4VtdHNiYYSgNVAhfU1iYX\0[ZIhOyCqcoOgZpkh\my3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBkd22yZYTpeIlwdiCjc4PhfUwhU2liPTCwMlAxOzRizszNMi=> NV22U3ZtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4NFc2OTJpPkKxPFA4PTF{PD;hQi=>
BL21 MYPGeY5kfGmxbjDhd5NigQ>? MYKxNEB2VQ>? NISxZog{KGi{cx?= MV3EbZNxdGGlZX3lcpQhd2ZiQl;ETXB[NUKjazDjc45rfWejdHXkJJBmeHSrZHWg[pJwdSCJU2SteIFo\2WmIHj1cYFvKEKlbD2yMYxqc2VicILveIVqdiBzIFWxNllJKG23dHHueEBmgHC{ZYPz[YQhcW5iRYPjbIVzcWOqaXGgZ49tcSCETEKxJINmdGy|IHH0JFExKHWPIHHmeIVzKDNiaILzJIJ6KG[udX;y[ZNk\W6lZTDwc4xiemm8YYTpc44h[2:vcHX0bZRqd25iYYPzZZktKEurIE2gNE4xODR3IN88UU4> NFi5dnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUiwO|UyOid-MkG4NFc2OTJ:L3G+
BL21 MofzSpVv[3Srb36gZZN{[Xl? MmLKNVAhfU1? MlH1N{BpenN? MYXEbZNxdGGlZX3lcpQhd2ZiQl;ETXB[NUKjazDjc45rfWejdHXkJJBmeHSrZHWg[pJwdSCJU2SteIFo\2WmIHj1cYFvKEKlbD2yMYxqc2VicILveIVqdiBzIFW5OmchdXW2YX70JIV5eHKnc4Pl[EBqdiCHc3Po[ZJq[2irYTDjc4xqKEKOMkGgZ4VtdHNiYYSgNVAhfU1iYX\0[ZIhOyCqcoOgZpkh\my3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBkd22yZYTpeIlwdiCjc4PhfUwhU2liPTCwMlAxPThizszNMi=> M3LuVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOEC3OVEzLz5{MUiwO|UyOjxxYU6=
BL21 M4PpRmZ2dmO2aX;uJIF{e2G7 MoG1NVAhfU1? NHW1XHo{KGi{cx?= NI\3RZdFcXOybHHj[Y1mdnRib3[gRm9FUVC\LVLhb{Bkd26sdXfheIVlKHCncITp[IUh\nKxbTDHV3QufGGpZ3XkJIh2dWGwIFLjcE0zNWyra3WgdJJwfGWrbjCxJGEyPDKJZHXseIEyOz[WIH31eIFvfCCneIDy[ZN{\WRiaX6gSZNkcGW{aXPobYEh[2:uaTDCUFIyKGOnbHzzJIF1KDFyIIXNJIFnfGW{IEOgbJJ{KGK7IH\seY9z\XOlZX7j[UBxd2yjcnn6ZZRqd25iY3;tdIV1cXSrb36gZZN{[XluIFvpJF0hOC5yMTFOwG0v MkX6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6MEe1NVIoRjJzOEC3OVEzRC:jPh?=
BL21 NFG1d5NHfW6ldHnvckBie3OjeR?= MYGxNEB2VQ>? M4j3ZVMhcHK| NY\6ZWZTTGm|cHzhZ4Vu\W62IH;mJGJQTEmSWT3CZYsh[2:waoXnZZRm\CCyZYD0bYRmKG[{b32gS3NVNXSjZ3fl[EBpfW2jbjDCZ4wuOi2uaXvlJJBzd3SnaX6gNUBCOTR{RzDteZRidnRiZYjwdoV{e2WmIHnuJGV{[2incnnjbIliKGOxbHmgRmwzOSClZXzsd{BifCBzMDD1UUBi\nSncjCzJIhzeyCkeTDmcJVwemW|Y3XuZ4UheG:uYYLpfoF1cW:wIHPvcZBmfGm2aX;uJIF{e2G7LDDLbUA:KDBwMEKxJO69VS5? MknpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6MEe1NVIoRjJzOEC3OVEzRC:jPh?=
BL21 MWfGeY5kfGmxbjDhd5NigQ>? M4q1S|ExKHWP NVq2TWl5OyCqcoO= NXPkcWh5TGm|cHzhZ4Vu\W62IH;mJGJQTEmSWT3CZYsh[2:waoXnZZRm\CCyZYD0bYRmKG[{b32gS3NVNXSjZ3fl[EBpfW2jbjDCZ4wuOi2uaXvlJJBzd3SnaX6gNUBNOTNyVjDteZRidnRiZYjwdoV{e2WmIHnuJGV{[2incnnjbIliKGOxbHmgRmwzOSClZXzsd{BifCBzMDD1UUBi\nSncjCzJIhzeyCkeTDmcJVwemW|Y3XuZ4UheG:uYYLpfoF1cW:wIHPvcZBmfGm2aX;uJIF{e2G7LDDLbUA:KDBwMEW4JO69VS5? MkPjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6MEe1NVIoRjJzOEC3OVEzRC:jPh?=
BL21 NFO4WYVHfW6ldHnvckBie3OjeR?= M1PnT|ExKHWP NUjTPYpoOyCqcoO= NVfIZXJwTGm|cHzhZ4Vu\W62IH;mJGJQTEmSWT3CZYsh[2:waoXnZZRm\CCyZYD0bYRmKG[{b32gS3NVNXSjZ3fl[EBpfW2jbjDCZ4wuOi2uaXvlJJBzd3SnaX6gNUBl\Wy2YUGzOnQhdXW2YX70JIV5eHKnc4Pl[EBqdiCHc3Po[ZJq[2irYTDjc4xqKEKOMkGgZ4VtdHNiYYSgNVAhfU1iYX\0[ZIhOyCqcoOgZpkh\my3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBkd22yZYTpeIlwdiCjc4PhfUwhU2liPTCwMlA3KM7:TT6= M1\5RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOEC3OVEzLz5{MUiwO|UyOjxxYU6=
BL21 M4S5bmZ2dmO2aX;uJIF{e2G7 NInie2IyOCC3TR?= NFfE[pI{KGi{cx?= Mny1SIl{eGyjY3Xt[Y51KG:oIFLPSGlRYS2EYXugZ49vcnWpYYTl[EBx\XC2aXTlJIZzd21iR2PUMZRi\2enZDDoeY1idiCEY3ytNk1tcWunIIDyc5RmcW5iMTDMNVMxSSCvdYThcpQh\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliQlyyNUBk\WyuczDheEAyOCC3TTDh[pRmeiB|IHjyd{BjgSCobIXvdoV{[2WwY3WgdI9t[XKrenH0bY9vKGOxbYDleIl1cW:wIHHzd4F6NCCNaTC9JFAvODd|IN88UU4> M2W5RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOEC3OVEzLz5{MUiwO|UyOjxxYU6=
BL21 M4TYfGZ2dmO2aX;uJIF{e2G7 MmjTNVAhfU1? M3PkOFMhcHK| MVXEbZNxdGGlZX3lcpQhd2ZiQl;ETXB[NUKjazDjc45rfWejdHXkJJBmeHSrZHWg[pJwdSCJU2SteIFo\2WmIHj1cYFvKEKlbD2yMYxqc2VicILveIVqdiBzIGKxNFBGKG23dHHueEBmgHC{ZYPz[YQhcW5iRYPjbIVzcWOqaXGgZ49tcSCETEKxJINmdGy|IHH0JFExKHWPIHHmeIVzKDNiaILzJIJ6KG[udX;y[ZNk\W6lZTDwc4xiemm8YYTpc44h[2:vcHX0bZRqd25iYYPzZZktKEurIE2gNE4yPiEQvF2u NHjhT|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUiwO|UyOid-MkG4NFc2OTJ:L3G+
BL21 MknMSpVv[3Srb36gZZN{[Xl? MVOxNEB2VQ>? NYC0ZYVPOyCqcoO= MWXEbZNxdGGlZX3lcpQhd2ZiQl;ETXB[NUKjazDjc45rfWejdHXkJJBmeHSrZHWg[pJwdSCJU2SteIFo\2WmIHj1cYFvKEKlbD2yMYxqc2VicILveIVqdiBzIG[xOFFCKG23dHHueEBmgHC{ZYPz[YQhcW5iRYPjbIVzcWOqaXGgZ49tcSCETEKxJINmdGy|IHH0JFExKHWPIHHmeIVzKDNiaILzJIJ6KG[udX;y[ZNk\W6lZTDwc4xiemm8YYTpc44h[2:vcHX0bZRqd25iYYPzZZktKEurIE2gNE4yQSEQvF2u MknPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6MEe1NVIoRjJzOEC3OVEzRC:jPh?=
BL21 MXrGeY5kfGmxbjDhd5NigQ>? M4HBcFExKHWP NF3jN2o{KGi{cx?= NYDRUYZxTGm|cHzhZ4Vu\W62IH;mJGJQTEmSWT3CZYsh[2:waoXnZZRm\CCyZYD0bYRmKG[{b32gS3NVNXSjZ3fl[EBpfW2jbjDCZ4wuOi2uaXvlJJBzd3SnaX6gNUBCOTR{VDDteZRidnRiZYjwdoV{e2WmIHnuJGV{[2incnnjbIliKGOxbHmgRmwzOSClZXzsd{BifCBzMDD1UUBi\nSncjCzJIhzeyCkeTDmcJVwemW|Y3XuZ4UheG:uYYLpfoF1cW:wIHPvcZBmfGm2aX;uJIF{e2G7LDDLbUA:KDBwMkeg{txONg>? M33QR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOEC3OVEzLz5{MUiwO|UyOjxxYU6=
BL21 M3X1dGZ2dmO2aX;uJIF{e2G7 MmnsNVAhfU1? NHHzN|U{KGi{cx?= NYrje2JOTGm|cHzhZ4Vu\W62IH;mJGJQTEmSWT3CZYsh[2:waoXnZZRm\CCyZYD0bYRmKG[{b32gS3NVNXSjZ3fl[EBpfW2jbjDCZ4wuOi2uaXvlJJBzd3SnaX6gNUBNOTNyRzDteZRidnRiZYjwdoV{e2WmIHnuJGV{[2incnnjbIliKGOxbHmgRmwzOSClZXzsd{BifCBzMDD1UUBi\nSncjCzJIhzeyCkeTDmcJVwemW|Y3XuZ4UheG:uYYLpfoF1cW:wIHPvcZBmfGm2aX;uJIF{e2G7LDDLbUA:KDBwMkmg{txONg>? M1;yU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOEC3OVEzLz5{MUiwO|UyOjxxYU6=
BL21 NEHZdmNHfW6ldHnvckBie3OjeR?= NFS0cnkyOCC3TR?= MlzPN{BpenN? NFrL[ZlFcXOybHHj[Y1mdnRib3[gRm9FUVC\LVLhb{Bkd26sdXfheIVlKHCncITp[IUh\nKxbTDHV3QufGGpZ3XkJIh2dWGwIFLjcE0zNWyra3WgdJJwfGWrbjCxJGY6P1ZibYX0ZY51KGW6cILld5Nm\CCrbjDFd4Np\XKrY3jpZUBkd2yrIFLMNlEh[2WubIOgZZQhOTBidV2gZYZ1\XJiMzDodpMh[nliZnz1c5Jme2OnbnPlJJBwdGG{aYrheIlwdiClb33w[ZRqfGmxbjDhd5NigSxiS3mgQUAxNjNizszNMi=> M4\2OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOEC3OVEzLz5{MUiwO|UyOjxxYU6=
BL21 MW\GeY5kfGmxbjDhd5NigQ>? NXjQNFZIOTBidV2= NH3uS5Q{KGi{cx?= NI\y[ZJFcXOybHHj[Y1mdnRib3[gRm9FUVC\LVLhb{Bkd26sdXfheIVlKHCncITp[IUh\nKxbTDHV3QufGGpZ3XkJIh2dWGwIFLjcE0zNWyra3WgdJJwfGWrbjCxJHkyODGKIH31eIFvfCCneIDy[ZN{\WRiaX6gSZNkcGW{aXPobYEh[2:uaTDCUFIyKGOnbHzzJIF1KDFyIIXNJIFnfGW{IEOgbJJ{KGK7IH\seY9z\XOlZX7j[UBxd2yjcnn6ZZRqd25iY3;tdIV1cXSrb36gZZN{[XluIFvpJF0hOC5|ODFOwG0v NEXQe5A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUiwO|UyOid-MkG4NFc2OTJ:L3G+
BL21 (DE3) M1vqcWZ2dmO2aX;uJIF{e2G7 MX[yJIhzew>? Mn\jRolv\GmwZzDh[oZqdmm2eTD0c{BPNXSncn3pcpV{KD[[IFjpd{11[WepZXSgbJVu[W5iQnPsNkBmgHC{ZYPz[YQhcW5iRYPjbIVzcWOqaXGgZ49tcSCETEKxJEhFTTNrIHPlcIx{KGGodHXyJFIhcHK|IHL5JIZtfW:{ZYPj[Y5k\SCyb3zhdol7[XSrb36gZZN{[XluIFvpJF0hOC5yMEC2JO69VS5? MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR2OEm4PEc,OjJ2NEi5PFg9N2F-
BL21 (DE3) MonESpVv[3Srb36gZZN{[Xl? NWL2VoJpOiCqcoO= NYjRfHhRSmmwZHnu[{Bi\m[rbnn0fUB1dyCQLYTldo1qdnW|IEjYJGhqey22YXfn[YQhcHWvYX6gRoNtNXiOIHX4dJJme3OnZDDpckBGe2OqZYLpZ4hq[SClb3zpJGJNOjFiKFTFN{kh[2WubIOgZYZ1\XJiMjDodpMh[nliZnz1c5Jme2OnbnPlJJBwdGG{aYrheIlwdiCjc4PhfUwhU2liPTCwMlAxOSEQvF2u M4CybFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NES4PVg5Lz5{MkS0PFk5QDxxYU6=
Toledo M1vq[GFxd3C2b4Ppd{Bie3OjeR?= M1y2b2lv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iVH;s[YRwKGOnbHzzMEBNTDVyIE2gNE4xPiEQvF2u M4HBU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwOlUzLz5{NEmwNFY2OjxxYU6=
KB M4DITGN6fG:2b4jpZ4l1gSCjc4PhfS=> MoWzNE42KHWP MWfDfZRwfG:6aXPpeJkhcW5iaIXtZY4he2mUTlGtcYVlcWG2ZXStUWNNOS2tbnPvb{Bld3ewIFvCJINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEKFTEKgZZQhOC53IIXN MnniQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThyNECwOFMoRjF6MESwNFQ{RC:jPh?=
Eu-Myc MYfBdI9xfG:|aYOgZZN{[Xl? MYWxJJVO NEDrS4FKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJI1wfXOnIFX1MW16[yClZXzsd{BwfmW{ZYjwdoV{e2mwZzDCR2wzKGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yh[2:ub375JIZwem2jdHnvckBifCBzIIXN MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB2MEC0N{c,OThyNECwOFM9N2F-
HeLa NX7BOGtYTnWwY4Tpc44h[XO|YYm= NIfIeJYyKHWP M3P3dVEzKGi{cx?= MWLJcoR2[3Srb36gc4YhSmOuLYjMMY1m\GmjdHXkJIFxd3C2b4Ppd{BqdiCmb4j5Z5lkdGmwLYP0bY12dGG2ZXSgbJVu[W5iSHXMZUBk\WyuczDveoVz\XiycnXzd4lv\yCQb4jhJIF1KDFidV2gZYZ1\XJiMUKgbJJ{KGK7IFjv[YNpe3Ric4ThbY5qdmd? MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN6Nkm4Nkc,OjJ|OE[5PFI9N2F-
HeLa NF;IZm9HfW6ldHnvckBie3OjeR?= NIi1WpkyOCC3TR?= M3zuZVE3KGi{cx?= MVnJcohq[mm2aX;uJI9nKFKudXOtRoF5N2W\RmCtRoNtNXiOIHnueIVz[WO2aX;uJIV5eHKnc4Pl[EBqdiCqdX3hckBJ\UyjIHPlcIx{KGG2IEGwJJVOKGGodHXyJFE3KGi{czDifUBDWkWWIHHzd4F6 NHO3V4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVA{OSd-MkK0NlUxOzF:L3G+
MDA-MB-231 M3n4U2Z2dmO2aX;uJIF{e2G7 M{Plb|AvODNidH:gNUB2VQ>? MmLONUBpeg>? M2LFV2FvfGGpb37pd5Qh[WO2aY\peJkh[XRicnXjc41jcW6jboSgRoNtNViOIHHzd4V{e2WmIHHzJJJme3SxcnH0bY9vKG:oIFLJUUBDUDNvaX7keYNm\CCVbXHjJJBzd3SnaX6gdoVt\WG|ZTDpckBucXSxY3jvcoRzcWFiaYPvcIF1\WRiZoLvcUBOTEFvTVKtNlMyKGOnbHzzJIF1KDBwMEOgeI8hOSC3TTDh[pRmeiBzIHjyJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd2N{W5PEc,OjJ5NEe1PVg9N2F-
MDA-MB-231 MmjCSpVv[3Srb36gZZN{[Xl? NHq0dZoxNjB|IITvJFEhfU1? MVexJIhz MmPJRY51[WexbnnzeEBi[3Srdnn0fUBifCC{ZXPvcYJqdmGwdDDCZ4wuYExiYYPz[ZN{\WRiYYOgdoV{fG:{YYTpc44hd2ZiQlnNJGJJOy2rbnT1Z4VlKGO7dH;jbJJwdWViYzDy[Yxm[XOnIHnuJI1qfG:laH;u[JJq[SCrc3;sZZRm\CCocn;tJG1FSS2PQj2yN|Eh[2WubIOgZZQhOC5yMzD0c{AyKHWPIHHmeIVzKDFiaIKgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| M1zoflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{S3OVk5Lz5{Mke0O|U6QDxxYU6=
BP3 NGTnPJpCeG:ydH;zbZMh[XO|YYm= NU\hdG5tOTBidV2= NXPFeVdCOjRiaILz MVLJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKEKSMzDj[YxteyCjdDCxNEB2VSCrbnP1ZoF1\WRiZn;yJFI1KGi{czDifUBCdm6neHnuJHYh[W6mIIDyc5Bq\Gm3bTDpc4Rq\GVic4ThbY5qdmdiYnHz[YQhTkGFUzDt[ZRpd2R? MnfoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNEeyNlgoRjJ|MES3NlI5RC:jPh?=
IM9 NF3vcFVCeG:ydH;zbZMh[XO|YYm= NHXVSJAyOCC3TR?= NXrxVG5rOjRiaILz M{fafGlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iSV25JINmdGy|IHH0JFExKHWPIHnuZ5Vj[XSnZDDmc5IhOjRiaILzJIJ6KEGwbnX4bY4hXiCjbnSgdJJweGmmaYXtJIlw\GmmZTDzeIFqdmmwZzDiZZNm\CCIQVPTJI1mfGixZB?= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB2N{KyPEc,OjNyNEeyNlg9N2F-
RS4:11 NHfpWmdCeG:ydH;zbZMh[XO|YYm= NWe5O5hyOTBidV2= MmLaNlQhcHK| M3np[Wlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iUmO0PlEyKGOnbHzzJIF1KDFyIIXNJIlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IFHucoV5cW5iVjDhcoQheHKxcHnkbZVuKGmxZHnk[UB{fGGrbnnu[{Bj[XOnZDDGRWNUKG2ndHjv[C=> NXzicYtTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOFczOjhpPkKzNFQ4OjJ6PD;hQi=>
HCT116 M3fPVGFxd3C2b4Ppd{Bie3OjeR?= M37YNVQ5KGi{cx?= NIe1O5RKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJIh2dWGwIFjDWFEyPiCyNUOrM{sh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IFHucoV5cW5iVj3GTXREKHO2YXnubY5oNWKjc3XkJIZtd3diY4n0c41mfHKrYzDt[ZRpd2R? MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjl6MkO3Nkc,OjZ7OEKzO|I9N2F-
DMS53 NHvlToxCeG:ydH;zbZMh[XO|YYm= MXi1JJRwKDFyIIXN NEj5XpMyOiCqcoO= MlnjTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCGTWO1N{Bk\WyuczDoZZJjd3KrbnegdFU{KG23dHHueEBie3Onc4Pl[EBieyCleYTvZ4hzd22nIHOgdoVt\WG|ZTDheEA2KHSxIEGwJJVOKGGodHXyJFEzKGi{czDifUBqdW23bn;icI91fGmwZzDt[ZRpd2R? MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjl6MkO3Nkc,OjZ7OEKzO|I9N2F-
DMS53 Mn7oSpVv[3Srb36gZZN{[Xl? NVXqUW5CPSC2bzCxNEB2VQ>? NVT2Z5pjOTJiaILz NV[zVIFYUW6qaXLpeIlwdiCxZjDCR4wuOi:EaX2gbY51\XKjY4Tpc44hcW5iaIXtZY4hTE2VNUOgZ4VtdHNiaHHyZo9zcW6pIIC1N{BufXSjboSgZZQhPSC2bzCxNEB2VSCjZoTldkAyOiCqcoOgZpkhcW2vdX7vdJJm[2myaYTheIlwdiCvZYToc4Q> NHrhbpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nkm4NlM4Oid-Mk[5PFI{PzJ:L3G+
DMS53 MXnBdI9xfG:|aYOgZZN{[Xl? NFr0eVI2KHSxIEGwJJVO MknFNVIhcHK| M3HaO2lv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iRF3TOVMh[2WubIOgbIFz[m:{aX7nJJA2OyCvdYThcpQh[XO|ZYPz[YQh[XNiUFHSVEBkdGWjdnHn[UBifCB3IITvJFExKHWPIHHmeIVzKDF{IHjyd{BjgSCrbX31co9jdG:2dHnu[{Bu\XSqb3S= M{jkOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUiyN|czLz5{Nkm4NlM4OjxxYU6=
DMS53 NFjwfopCeG:ydH;zbZMh[XO|YYm= M2mzWlUhfG9iMUCgeW0> M1z2SlEzKGi{cx?= NULyVnBrUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDEUXM2OyClZXzsd{Bp[XKkb4LpcocheDV|IH31eIFvfCCjc4Pld5Nm\CCjczDjZZNx[XOnLUOgZ4xm[X[jZ3WgZZQhPSC2bzCxNEB2VSCjZoTldkAyOiCqcoOgZpkhcW2vdX7vZoxwfHSrbnegcYV1cG:m NWixWGpGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[5PFI{PzJpPkK2PVgzOzd{PD;hQi=>
DMS53 MlP1SpVv[3Srb36gZZN{[Xl? NFvFPWo2KHSxIEGwJJVO NFLafpAyOiCqcoO= NGLkTXVKdmirYnn0bY9vKG:oIFLDcE0zN0KjeDDpcpRmemGldHnvckBqdiCqdX3hckBFVVN3MzDj[YxteyCqYYLic5JqdmdicEWzJI12fGGwdDDheEA2KHSxIEGwJJVOKGGodHXyJFEzKGi{czDifUBqdW23bn;wdoVkcXCrdHH0bY9vKG2ndHjv[C=> MojWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ7OEKzO|IoRjJ4OUiyN|czRC:jPh?=
U-2 OS NYDnfVM4eUiWUzDhd5NigQ>? NVv0S5p3eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGWtNkBQWyClZXzsdy=> Ml:5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
A673 NF[2cJhyUFSVIHHzd4F6 MnLidWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEF4N{OgZ4VtdHN? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
DAOY NHO5fYFyUFSVIHHzd4F6 M{TUXZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCGQV;ZJINmdGy| NXfiNo9HRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Saos-2 NWHhUJJzeUiWUzDhd5NigQ>? NYLSeGFUeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPhc5MuOiClZXzsdy=> NGD1OYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
RD NHHme2lyUFSVIHHzd4F6 M1TIZ5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCURDDj[Yxtew>? NF:zeJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-SH MV7xTHRUKGG|c3H5 NWfpZlJleUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPLMW4uW0hiY3XscJM> NUHRT3c3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
NB1643 Mk\hdWhVWyCjc4PhfS=> MkXDdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6EMU[0N{Bk\Wyucx?= M1myfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
OHS-50 M2T6[JFJXFNiYYPzZZk> NH;BUWZyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiT1jTMVUxKGOnbHzz MoPtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
LAN-5 MkPXdWhVWyCjc4PhfS=> NYn0elI6eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhVEGQLUWgZ4VtdHN? MkKzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
DAOY NGP0e4pyUFSVIHHzd4F6 MlHqdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgSGFQYSClZXzsdy=> NEX6WFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
NB-EBc1 M1;kRpFJXFNiYYPzZZk> NXHoTppteUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhVkJvRVLjNUBk\Wyucx?= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh41 MX\xTHRUKGG|c3H5 NXPIdItbeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLoOFEh[2WubIO= M{HaVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
A673 MljodWhVWyCjc4PhfS=> MXfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDBOlc{KGOnbHzzLS=> MlvuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Rh30 MkHZdWhVWyCjc4PhfS=> NHzJbY5yUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUnizNEBk\Wyucx?= NFrkTXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
MG 63 (6-TG R) MmHkdWhVWyCjc4PhfS=> M1jKdZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJG1IKDZ|IDi2MXRIKFJrIHPlcIx{ Mo\6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
U-2 OS NYX5eIdXeUiWUzDhd5NigQ>? MlG2dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgWU0zKE:VIHPlcIx{ NY\J[YNCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Rh41 NVXTNGIzeUiWUzDhd5NigQ>? MXrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDSbFQyKGOnbHzz MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SJ-GBM2 NUTjR|JLeUiWUzDhd5NigQ>? NIrW[3hyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1qtS2JOOiClZXzsdy=> NXXtVoN1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-MC NGDnTXNyUFSVIHHzd4F6 MoPQdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tUWMh[2WubIO= M2rj[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB-EBc1 Mn;BdWhVWyCjc4PhfS=> NIrnbZlyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTlKtSWJkOSClZXzsdy=> NFnBSnc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
LAN-5 NWn2dGZbeUiWUzDhd5NigQ>? MWXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVEGQLUWgZ4VtdHN? NEDIeWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Rh18 M4jVWZFJXFNiYYPzZZk> MXXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmhzODDj[Yxtew>? M2LJOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB1643 MlvPdWhVWyCjc4PhfS=> NUjMenNzeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhVkJzNkSzJINmdGy| NELoZ|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-MC NUXMU49MeUiWUzDhd5NigQ>? M3PLbZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHNMNU5vTVOgZ4VtdHN? NEO0dmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
TC32 NGrp[lByUFSVIHHzd4F6 NHLIZmtyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBVSzN{IHPlcIx{ M3\DTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh18 M{TnOZFJXFNiYYPzZZk> NE\HPGtyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBTcDF6IHPlcIx{ NG\RUII9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Saos-2 M1XURpFJXFNiYYPzZZk> M{XKRZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHNid3NvMjDj[Yxtew>? NETpfGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
PARP / c-PARP / cleaved caspase 3 ; 

PubMed: 21393866     


H146 cells were preincubated in normoxia or hypoxia (1% O2) for 18 hours prior to treatment with ABT-737 (89.1 nM ), after which cells were harvested for protein analysis by Western blot. 

Hif-1a; 

PubMed: 22006676     


Representative western blots for HIF-1α, PARP and cleaved caspase 3 in response to 24-72 hr exposure to ABT-737 in varying concentration in normoxia and hypoxia in 4 neuroblastoma cell lines. Actin is shown as a loading control. Blots are representative of 3 independent experiments.

γ-H2AX / p-ATM; 

PubMed: 21084274     


Induction of γ-H2AX and p-ATM by ABT-737. Cells were treated with 10 µmol/L ABT-737 for 24 hours and then maintained in fresh media for 1 or 2 more days prior to Western blot analysis.

21393866 22006676 21084274
Immunofluorescence
cytochrome C; 

PubMed: 26447615     


Immunocytochemistry revealed the release of mitochondrial cytochrome c (green) into the cytoplasm after ABT-737 exposure (20 μM in MC-3 cells and 22.5 μM in HN22 cells) for 24 hr. MitoTracker (red) staining labels mitochondria within each field.

Bax; 

PubMed: 26447615     


Immunocytochemistry revealed the mitochondrial translocation of Bax from the cytoplasm after ABT-737 treatment (20 μM in MC-3 cells and 22.5 μM in HN22 cells) for 24 hr. MitoTracker (red) staining labels mitochondria within each field C. To assess whether ABT-737 treatment has an effect on the dimerization of Bax, the levels of dimeric Bax were measured with or without ABT-737 treatment in MC-3 and HN22 cells.

Bim; 

PubMed: 26447615     


Immunocytochemistry revealed the mitochondrial translocation of Bim from the cytoplasm after ABT-737 treatment (20 μM in MC-3 cells and 22.5 μM in HN22 cells) for 24 hr. MitoTracker (red) staining labels mitochondria within each field.

AIF; 

PubMed: 25034785     


localization of apoptosis inducing factor (AIF) in LCSC pre-stained with Mitotracker Red and untreated or treated with 500 nM ABT-737 for 48 h. Magnification × 60, zoom × 3, bar 20 μm. 

p65 ; 

PubMed: 28846096     


BCL-xL dependent SVEC cells were treated with ABT-737 (10 μM) +/- Q-VD-Oph (30 μM) for 1 h, immunostained for p65 and analysed by confocal microscopy. TNF (20 ng/ml) was used as positive control for p65 nuclear translocation. Scale bar represents 30μM. Representative images from three independent experiments.

26447615 25034785 28846096
Growth inhibition assay
Cell viability; 

PubMed: 22311987     


Stably transfected K562 cells were treated with increasing concentrations of ABT-737, obatoclax, or maritoclax for 24 h. Cell viability was determined by measuring intracellular ATP levels with the CellTiter Glo assay.

22311987
ELISA
IL-6 / IL-8; 

PubMed: 21084274     


The secretion of IL-6 and IL-8 in PV-10 cells treated with DMSO, ABT-737 or enantiomer for 24 hours was determined by ELISA.

21084274
In vivo In aggressive leukemia model, ABT-737 suppresses the leukemia burden by 53% at the 30 mg/kg, with significantly extended survival of mice. ABT-737 does not induce significantly abnormalities in blood cell counts or serum chemistries. [1] ABT-737 prolongs the survival of recipient mice transplanted with Bcl-2-transduced tumors. [2] ABT-737 shows great antitumor activity in an ATLL mouse model at a dose of 100 mg/kg. [5]

Protocol

Kinase Assay:

[1]

- Collapse

Fluorescence polarization assays:

Binding affinity of GST-Bcl-2 family proteins to the FITC-conjugated BH3 domain of Bim (FITC-Ahx-DMRPEIWIAQELRRIGDEFNAYYAR) is determined. Briefly, 100 nM of GST-Bcl-2 family fusion proteins are incubated with serial dilutions of ABT-737 in PBS for 2 min. Then, 20 nM of FITC-Bim BH3 peptide (FITC-Ahx-DMRPEIWIAQELRRIGDEFNAYYAR) is added. Fluorescence polarization is measured using an Analyst TM AD Assay Detection System after 10 min using the 96-well black plate. Then IC50 are determined.
Cell Research:

[4]

- Collapse
  • Cell lines: SCLC cell lines NCI-H889, NCI-H1963, NCI-H1417, NCI-H146, NCI-187, DMS79, NCI-1048, NCI-H82, NCI-H196, H69AR, and DMS114
  • Concentrations: 0.001-10 μM
  • Incubation Time: 48 hours
  • Method:

    SCLC cells are treated for 48 hours in 96-well tissue culture plates in a total volume of 100 μL tissue culture medium supplemented with 10% human serum. Viable cells are determined using the MTS assay.


    (Only for Reference)
Animal Research:

[1]

- Collapse
  • Animal Models: Scid mice injected with Luc-expressing FD/ΔRaf-1:ER cells
  • Dosages: 20 and 30 mg/kg
  • Administration: For intraperitoneal (i.p.) every day
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (122.93 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2%DMSO+40%PEG300+5%Tween80+53%ddH2O
For best results, use promptly after mixing.
2mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 813.43
Formula

C42H45ClN6O5S2

CAS No. 852808-04-9
Storage powder
in solvent
Synonyms N/A
Smiles CN(C)CCC(CSC1=CC=CC=C1)NC2=C(C=C(C=C2)S(=O)(=O)NC(=O)C3=CC=C(C=C3)N4CCN(CC4)CC5=CC=CC=C5C6=CC=C(C=C6)Cl)[N+](=O)[O-]

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    What’s the recommended method about reconstitution of the compound for in vivo animal study?

  • Answer:

    For oral administration, we suggest the vehicle: 30% Propylene glycol, 5% Tween 80, 65% D5W, at up to 30mg/ml; For injection, ABT-737 can be dissolved in 2% DMSO/50% PEG 300/5% Tween 80/ddH2O at 2.5 mg/ml.

Bcl-2 Signaling Pathway Map

Bcl-2 Inhibitors with Unique Features

Related Bcl-2 Products

Tags: buy ABT-737 | ABT-737 supplier | purchase ABT-737 | ABT-737 cost | ABT-737 manufacturer | order ABT-737 | ABT-737 distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID