Navitoclax (ABT-263)

For research use only.

Catalog No.S1001

259 publications

Navitoclax (ABT-263) Chemical Structure

CAS No. 923564-51-6

Navitoclax (ABT-263) is a potent inhibitor of Bcl-xL, Bcl-2 and Bcl-w with Ki of ≤ 0.5 nM, ≤1 nM and ≤1 nM in cell-free assays, but binds more weakly to Mcl-1 and A1. Phase 2.

Selleck's Navitoclax (ABT-263) has been cited by 259 publications

Purity & Quality Control

Choose Selective Bcl-2 Inhibitors

Biological Activity

Description Navitoclax (ABT-263) is a potent inhibitor of Bcl-xL, Bcl-2 and Bcl-w with Ki of ≤ 0.5 nM, ≤1 nM and ≤1 nM in cell-free assays, but binds more weakly to Mcl-1 and A1. Phase 2.
Targets
Bcl-xL [1]
(Cell-free assay)
Bcl-2 [1]
(Cell-free assay)
Bcl-w [1]
(Cell-free assay)
<=0.5 nM(Ki) <=1 nM(Ki) <=1 nM(Ki)
In vitro

ABT-263 is structurally related to ABT-737; it is a disruptor of Bcl-2/Bcl-xL interactions with pro-apoptotic proteins. Overexpression of the prosurvival Bcl-2 family members is commonly associated with tumor maintenance, progression, and chemoresistance. [1] ABT-263 displays the protection afforded by overexpression of Bcl-2 or Bcl-xL with EC50 values of 60 nM and 20 nM, respectively. [1] A wide range of cellular activity is observed with ABT-263 having a 50% growth inhibition (EC50) of 110 nM against the most sensitive line (H146), whereas its activity in the least sensitive line (H82) results in an EC50 at 22 μM. All four cell lines with EC50 values of <400 nM (H146, H889, H1963, and H1417) are also highly sensitive to ABT-737, and the two most resistant lines (H1048 and H82) are similarly resistant to ABT-263. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
FL5.12 M{HyfGN6fG:2b4jpZ4l1gSCjc4PhfS=> NX7FNVltOjRiaB?= MnfaSWM2OCB;IECuNFA1OiEQvF2= NVS1eGprRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi4OFE5QDJpPkG4PFQyQDh{PD;hQi=>
FL5.12 Mn23R5l1d3SxeHnjbZR6KGG|c3H5 MWSyOEBp NUD4XpExTUN3MDC9JFAvODB3OTFOwG0> NWLFbGw5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi4OFE5QDJpPkG4PFQyQDh{PD;hQi=>
RS4:11 MonrRZBweHSxc3nzJIF{e2G7 NHHIUFc3KGh? MlK5SWM2OCB;IECuNFE1KM7:TR?= M{LtUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEixOVY4Lz5{NEi4NVU3PzxxYU6=
FDC-P1 MVrCR2wuOiCrbnjpZol1cW:wIHHzd4F6 MkPjNlQhcA>? Ml7RTWM2OCB;IECuNFE2KM7:TR?= NIm3c2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi4NVU3Pyd-MkS4PFE2Pjd:L3G+
NCI-H1963 M1nKbGN6fG:2b4jpZ4l1gSCjc4PhfS=> MVK0JIRigXN? NHraSppKSzVyIE2gNE4xOjZ4IN88US=> NYfnS4J4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OFgzQThpPkKzOFQ5Ojl6PD;hQi=>
NCI-H187 MU\DfZRwfG:6aXPpeJkh[XO|YYm= M4HsUVQh\GG7cx?= MYHJR|UxKD1iMD6wN|g1KM7:TR?= NHHZbm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S0PFI6QCd-MkO0OFgzQTh:L3G+
NCI-H1417 MX;DfZRwfG:6aXPpeJkh[XO|YYm= NEDKeGY1KGSjeYO= NWTGc|FrUUN3MDC9JFAvODV2MjFOwG0> MnjTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NEiyPVgoRjJ|NES4Nlk5RC:jPh?=
FDC-P1 MnLYRmNNNXiOIHnubIljcXSrb36gZZN{[Xl? NGPMVJczPCCq MXPJR|UxKD1iMD6wOkDPxE1? M{fKNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEixOVY4Lz5{NEi4NVU3PzxxYU6=
NCI-H146 MkL6R5l1d3SxeHnjbZR6KGG|c3H5 MVG0PEBp NFrm[HRGSzVyIE2gNE4xQDZ5IN88US=> NF7zRpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEi0NVg5Oid-MUi4OFE5QDJ:L3G+
RS4:11 MYTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MXjFR|UxKD1iMD6xNVIh|ryP NH3aZmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEmyOlI1Pyd-Mki5NlYzPDd:L3G+
MOLT4 M13Ze2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NYPEOG1GTUN3MDC9JFAvOzB|IN88US=> MnHQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7Mk[yOFcoRjJ6OUK2NlQ4RC:jPh?=
EoL-1-cell NXnmdolWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3vve2lEPTB;MD6wNFY3QSEQvF2= M1LtZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
MV-4-11 NUKzW|FyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV:3U3NSUUN3ME2wMlAyPTh4IN88US=> M1jhe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
NKM-1 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{jrZ2lEPTB;MD6wNVY6QSEQvF2= M33kRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
ML-2 MljuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn7lTWM2OD1yLkCxPVg{KM7:TR?= M3fXSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
BV-173 NVnnfllkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUHzSFd5UUN3ME2wMlAzOzF2IN88US=> M2LCVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
RS4-11 NX\odWVlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXzJR|UxRTBwMEK1PFch|ryP M{PwPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
HL-60 M1nwS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlvsTWM2OD1yLkCyPVA5KM7:TR?= MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
KY821 M2P3OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnvuTWM2OD1yLkCyPVc2KM7:TR?= NWm0PJJiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
ECC10 MnmyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYHVVW5NUUN3ME2wMlA{Pzl{IN88US=> NEewdZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
NCI-H720 NWeyTVdNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWHJR|UxRTBwMESwNVEh|ryP NFrVZW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
QIMR-WIL NX;VcWhYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3;6XmlEPTB;MD6wOFI5PyEQvF2= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
KG-1 MmP3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYnI[lZLUUN3ME2wMlA1PDh4IN88US=> NIfDOHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
TGW M33yRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHjKeYxKSzVyPUCuNFQ3OzNizszN MnPqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
ATN-1 NXPDdIdjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUOzeWNoUUN3ME2wMlA1PzN|IN88US=> NEW2T5k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
RH-18 Ml7qS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFTnd4NKSzVyPUCuNFYxPDhizszN MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
EW-18 NX61[IhIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHHifnNKSzVyPUCuNFY5PDFizszN MmfMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
NB17 M3i0[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVvxU5lEUUN3ME2wMlA4OTJ2IN88US=> NILNSJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
SK-NEP-1 MkLJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1\uVGlEPTB;MD6wO|IyOyEQvF2= MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
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KARPAS-45 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFvs[3RKSzVyPUCuNFc5OTVizszN MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
EW-3 Mni5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGPHcGdKSzVyPUCuNFgxPTNizszN NGrsbFA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
NB13 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY\OWJQ4UUN3ME2wMlA5OjB|IN88US=> M{PwXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
NCI-H209 NWrKcpRmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnTzTWM2OD1yLkC4O|A1KM7:TR?= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
NCI-H1092 MkLXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYG1UmNSUUN3ME2wMlExOjd3IN88US=> Mlu1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
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697 MnHwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1rwTmlEPTB;MD6xNFg{QSEQvF2= MnWyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
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MOLT-4 NUjRXGFET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEe0PZRKSzVyPUCuNVUyPjlizszN M3XzfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
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IA-LM NUHKPWlzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml;ITWM2OD13LkO5PFcyKM7:TR?= MonmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
KP-4 M3u4V2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTVwNE[zN|Qh|ryP NGLNOW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
G-402 M{T6[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmG0TWM2OD13LkWxPFY2KM7:TR?= NX34[4pkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
OS-RC-2 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTVwNUK2NFQh|ryP M2LRclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
NCI-H1155 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWrJR|UxRTVwNUS5OVUh|ryP MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
OE19 MmXPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnXlTWM2OD13Lk[4OlI1KM7:TR?= MkG1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
U-2-OS NVTpPJdQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUDyNJVoUUN3ME21Mlg6ODF|IN88US=> NXjJXWVyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
SCC-15 M1LsSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2LTTGlEPTB;NT65N|Y3OiEQvF2= M2DKTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
NCI-H630 NIHQNpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTVwOUm0NFQh|ryP NFG1VIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
PFSK-1 NW\3UW51T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmPlTWM2OD14LkC1NlU6KM7:TR?= MnLYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
NCI-H1770 NYT3XIRLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1jwOWlEPTB;Nj6yNFg4PCEQvF2= NITEN3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
SK-MEL-3 M1rNS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEK0NoVKSzVyPU[uOFI6OTVizszN MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
LB1047-RCC NF:yOWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NI\pU|NKSzVyPU[uOFc3OjVizszN NU\rd5RsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
NCI-H446 MmrIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnvMTWM2OD14Lk[yPVI2KM7:TR?= Mny4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
SW780 M13JN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHi4OohKSzVyPU[uO|AyQDVizszN NIe2VVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
NEC8 NE\TdWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWTNWZNFUUN3ME22Mlc3PjNizszN MlLmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
NOMO-1 NWXJUolST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLhTWM2OD14Lke4NVEyKM7:TR?= NXLreWszRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
COLO-668 Mm[wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlPhTWM2OD14Lki0N|g4KM7:TR?= M{fSfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
MC116 M3H5W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn;xTWM2OD14LkmzPFk4KM7:TR?= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
HCC1937 M1vFWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NInjd2pKSzVyPU[uPVkzPTFizszN NYnJO5o{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
NCI-N87 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlLZTWM2OD15LkG5Nlk{KM7:TR?= MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
COLO-320-HSR NGHDU4pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4j2V2lEPTB;Nz6yNlc{QCEQvF2= M4K4[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
HCC1806 NXe2eXByT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1m2OmlEPTB;Nz6yOlA1PCEQvF2= NFXiTFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
OVCAR-3 M2CwZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWrJR|UxRTdwM{OwN|gh|ryP M4Lyb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
NUGC-3 NF:zPVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEixZ4lKSzVyPUeuN|k3QTRizszN MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
SW1783 NFnnWJlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWPqSoJyUUN3ME23MlQ{OTd3IN88US=> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
GCT MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF7uUWZKSzVyPUeuOVY6ODZizszN Mln5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
NCI-H2126 NFi1NXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3XubmlEPTB;Nz63N|YzPSEQvF2= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
MEL-HO M1;YXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVHlbI9MUUN3ME23Mlc4ODV2IN88US=> Mk\tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
CAPAN-1 NXfSPY5ZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXHJR|UxRTdwN{ezOVch|ryP NWHRN3Z3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
SW756 M{nPdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUHJR|UxRTdwN{izN|Mh|ryP NHn6VoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
SKG-IIIa MmDqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYm3PXpGUUN3ME23MlgyQDl{IN88US=> NXLqVphvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
HCE-T NEjyR5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1O5c2lEPTB;Nz64O|c5OyEQvF2= NFu2RYk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
Ca-Ski MknNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIj5[5NKSzVyPUeuPVk{QDNizszN NIG0cYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
COLO-684 NVznTWk6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWfzb5hpUUN3ME24MlAyQDF6IN88US=> M4LOc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
KYSE-70 M2LuWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4LwR2lEPTB;OD6wO|czQSEQvF2= NVjKPXJRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
TI-73 MkCyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn2xTWM2OD16LkK1PFUyKM7:TR?= NX;RT5o2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
BT-20 NF\h[5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVTJR|UxRThwMk[wOVIh|ryP M3XLd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
MHH-ES-1 NHvEUmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV;5[Gk6UUN3ME24MlUyQDN2IN88US=> MnuwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
TE-12 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1XCVWlEPTB;OD61PVk{OSEQvF2= NEnNdVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
YH-13 M1vJ[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUjYUm1LUUN3ME24MlYyODB6IN88US=> Mn3vQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
SF126 MkfrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVrmOWRZUUN3ME24Mlg{QDZ3IN88US=> MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
J82 NXfrNmFkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWXLR4hGUUN3ME24MlkxODN6IN88US=> NXvwZoloRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
RCC10RGB M2nFW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVvYZmFWUUN3ME24Mlk6PTZzIN88US=> NYjxS3Z7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
SK-UT-1 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlvQTWM2OD17LkC0PVQ2KM7:TR?= NUn5UXg2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
LB2241-RCC NWq1OYZpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmS2TWM2OD17LkG5NVM4KM7:TR?= M4m4dFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
LB996-RCC NF;TRXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIXGNmlKSzVyPUmuNVk5QSEQvF2= M2LRblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
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HT-1080 Mnj1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4HXSWlEPTB;OT63O|c{QSEQvF2= NEPZOpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
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SW948 MnfFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX7SPI1pUUN3ME20O{41PjRizszN MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
BALL-1 MmrBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYPJR|UxRTR5Lk[xOlgh|ryP NHnITlA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
TE-9 M3The2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXvaTmFLUUN3ME20O{46PThzIN88US=> NXXh[2tFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
SK-N-FI NYfuZ3lyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHHv[WdKSzVyPUS4MlA{PThizszN M2XQT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
KALS-1 MkLOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYDJR|UxRTR6LkGyPFkh|ryP NUPienZGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
HO-1-N-1 NUDGdXNVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTR6Lke0OFUh|ryP MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
NCI-H2452 MnPHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUL0[2o6UUN3ME20PU4yOTV{IN88US=> MoG5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
OC-314 NILPUVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX[0O4Q{UUN3ME20PU43QDN2IN88US=> NF7UUYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
STAT3 / pSTAT3 / STAT6 / pSTAT6 / Mcl-1 / Cleaved Mcl-1 / Bcl-xl / Bim / puma / Bik / Bax / PARP / c-Myc; 

PubMed: 26811457     


(A) Western blot analysis of expressions of STAT3/p-STAT3 and STAT6/p-STAT6 as well as antiapoptotic members (Bcl-2, Bcl-xL, and Mcl-1) after 48 h of treatments in HDLM-2 cells. (B) Western blot analysis of expressions of proapoptotic proteins (Bim, Puma, Bik, Bax, and p-Bad) as well as caspase cascade molecules (PARP and c-Myc) after 48 h of treatments. β-Actin was used as an input control.

caspase3 / cleaved caspase 3; 

PubMed: 24481442     


(b) (i) N/TERT, (ii) SINCE and (iii) SINEG2 cells were either untreated (0 μM/DMSO) or treated with increasing doses of Navitoclax (ABT-263; 0.025–30 μM) and were incubated for 24 h. Next, cells were collected and whole-cell lysates were prepared and immunoblotted against anti-full-length Caspase 3 (∼35 kDa) antibody, whereas β-actin (∼42 kDa) was used as a protein-loading control. Apoptosis was assessed through detecting the cleaved bands of Caspase-3 (∼19, 17 kDa). 

26811457 24481442
Growth inhibition assay
Cell viability; 

PubMed: 25685063     


(C) Hcc827, (D) H292, and (E) H1993 cells were treated with increasing concentrations of albendazole or mebendazole in the presence of navitoclax (0, 1, 2, and 4 μM). Viability was determined after 1 day.

25685063
In vivo When ABT-263 is administered at 100 mg/kg/day in the H345 xenograft model, significant antitumor efficacy is observed with 80% TGI and 20% of treated tumors indicating at least a 50% reduction in tumor volume. [2] Oral administration of ABT-263 alone causes complete tumor regressions in xenograft models of small-cell lung cancer and acute lymphoblastic leukemia. In xenograft models of aggressive B-cell lymphoma and multiple myeloma where ABT-263 displays modest or no single agent activity, it significantly enhances the efficacy of clinically relevant therapeutic regimens. [2]

Protocol

Kinase Assay:

[1]

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Affinity determination:

Binding affinities (Ki or IC50) of ABT-263 against different isoforms of Bcl-2 family are determined with competitive fluorescence polarization assays. The following peptide probe/protein pairs are used: f-bad (1 nM) and Bcl-xL (6 nM), f-Bax (1 nM) and Bcl-2 (10 nM), f-Bax (1 nM) and Bcl-w (40 nM), f-Noxa (2 nM) and Mcl-1 (40 nM), and f-Bax (1 nM) and Bcl-2-A1 (15 nM). Binding affinities for Bcl-xL are also determined using a time-resolved fluorescence resonance energy transfer assay. Bcl-xL (1 nM, His tagged) is mixed with 200 nM f-Bak, 1 nM Tb-labeled anti-His antibody, and ABT-263 at room temperature for 30 min. Fluorescence is measured on an Envision plate reader using a 340/35 nm excitation filter and 520/525 (f-Bak) and 495/510 nm (Tb-labeled anti-His antibody) emission filters.
Cell Research:

[1]

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  • Cell lines: SCLC cell lines
  • Concentrations: 0-1 μM
  • Incubation Time: 48 hours
  • Method:

    Human tumor cell lines SCLC cell lines are maintained at 37℃ containing 5% CO2. SCLC cell lines are cultured in RPMI 1640 with 10% fetal bovine serum (FBS), 1% sodium pyruvate, 25 mM HEPES, 4.5 g/L glucose, and 1% penicillin/streptomycin. Leukemia and lymphoma cell lines are cultured in RPMI 1640 supplemented with 10% FBS and 1% penicillin/streptomycin. Cells (1-5×10 4) are treated by ABT-263 for 48 hours in 96-well culture plates in a final volume of 100 μL and cytotoxicity is assessed with the CellTiter Glo assay. In vitro cyto toxicity of ABT-263 is assayed.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: C.B.-17 scid-bg or C.B.-17 scid mice
  • Dosages: 100 mg/kg/d
  • Administration: Administered via p.o.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (102.6 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+corn oil
For best results, use promptly after mixing.
4mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 974.61
Formula

C47H55ClF3N5O6S3

CAS No. 923564-51-6
Storage powder
in solvent
Synonyms N/A
Smiles CC1(CCC(=C(C1)CN2CCN(CC2)C3=CC=C(C=C3)C(=O)NS(=O)(=O)C4=CC(=C(C=C4)NC(CCN5CCOCC5)CSC6=CC=CC=C6)S(=O)(=O)C(F)(F)F)C7=CC=C(C=C7)Cl)C

In vivo Formulation Calculator (Clear solution)

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Bio Calculators

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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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The Serial Dilution Calculator Equation

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Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04480086 Active not recruiting Drug: Mivebresib|Drug: Navitoclax|Drug: Ruxolitinib Myelofibrosis (MF) AbbVie March 17 2021 Phase 1
NCT04454658 Recruiting Drug: ABBV-744|Drug: Navitoclax|Drug: Ruxolitinib Myelofibrosis (MF) AbbVie November 11 2020 Phase 1
NCT04041050 Recruiting Drug: Navitoclax|Drug: Ruxolitinib|Drug: Celecoxib Myeloproliferative Neoplasm AbbVie November 8 2019 Phase 1
NCT03181126 Completed Drug: Navitoclax|Drug: Chemotherapy|Drug: Venetoclax Acute Lymphoblastic Leukemia (ALL)|Lymphoblastic Lymphoma AbbVie November 27 2017 Phase 1
NCT02520778 Active not recruiting Drug: Navitoclax|Drug: Osimertinib Advanced Lung Non-Squamous Non-Small Cell Carcinoma|Metastatic Lung Non-Squamous Non-Small Cell Carcinoma|Stage III Lung Non-Small Cell Cancer AJCC v7|Stage IIIA Lung Non-Small Cell Cancer AJCC v7|Stage IIIB Lung Non-Small Cell Cancer AJCC v7|Stage IV Lung Non-Small Cell Cancer AJCC v7 National Cancer Institute (NCI) March 31 2016 Phase 1
NCT02591095 Completed Drug: ABT-263 Platinum-resistant or Refractory Ovarian Cancer Centre Francois Baclesse|ARCAGY/ GINECO GROUP|French Cancer Research Hospital Program January 2016 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I would like to deliver S1001 via i.p. injection, can you suggest a vehicle solution?

  • Answer:

    S1001 ABT-263 (Navitoclax) can be dissolved in 5% DMSO+corn oil at 7mg/ml as a clear solution.

  • Question 2:

    Could you tell me if the catalog number S1001 can go through the hemato-encephalic barrier?

  • Answer:

    According to the paper: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3223694/, ABT-263 can not cross brain-blood barrier.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID