Navitoclax (ABT-263)

For research use only.

Catalog No.S1001

268 publications

Navitoclax (ABT-263) Chemical Structure

CAS No. 923564-51-6

Navitoclax (ABT-263) is a potent inhibitor of Bcl-xL, Bcl-2 and Bcl-w with Ki of ≤ 0.5 nM, ≤1 nM and ≤1 nM in cell-free assays, but binds more weakly to Mcl-1 and A1. Phase 2.

Selleck's Navitoclax (ABT-263) has been cited by 268 publications

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Choose Selective Bcl-2 Inhibitors

Biological Activity

Description Navitoclax (ABT-263) is a potent inhibitor of Bcl-xL, Bcl-2 and Bcl-w with Ki of ≤ 0.5 nM, ≤1 nM and ≤1 nM in cell-free assays, but binds more weakly to Mcl-1 and A1. Phase 2.
Targets
Bcl-xL [1]
(Cell-free assay)
Bcl-2 [1]
(Cell-free assay)
Bcl-w [1]
(Cell-free assay)
<=0.5 nM(Ki) <=1 nM(Ki) <=1 nM(Ki)
In vitro

ABT-263 is structurally related to ABT-737; it is a disruptor of Bcl-2/Bcl-xL interactions with pro-apoptotic proteins. Overexpression of the prosurvival Bcl-2 family members is commonly associated with tumor maintenance, progression, and chemoresistance. [1] ABT-263 displays the protection afforded by overexpression of Bcl-2 or Bcl-xL with EC50 values of 60 nM and 20 nM, respectively. [1] A wide range of cellular activity is observed with ABT-263 having a 50% growth inhibition (EC50) of 110 nM against the most sensitive line (H146), whereas its activity in the least sensitive line (H82) results in an EC50 at 22 μM. All four cell lines with EC50 values of <400 nM (H146, H889, H1963, and H1417) are also highly sensitive to ABT-737, and the two most resistant lines (H1048 and H82) are similarly resistant to ABT-263. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
FL5.12 NYP0Wo9mS3m2b4TvfIlkcXS7IHHzd4F6 NH\CWG0zPCCq MnfxSWM2OCB;IECuNFA1OiEQvF2= NFH5dHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEi0NVg5Oid-MUi4OFE5QDJ:L3G+
FL5.12 MWDDfZRwfG:6aXPpeJkh[XO|YYm= M1TqbVI1KGh? NILndVdGSzVyIE2gNE4xODV7IN88US=> M4nNSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OESxPFgzLz5zOEi0NVg5OjxxYU6=
RS4:11 M1vFSWFxd3C2b4Ppd{Bie3OjeR?= NYjDU4gxPiCq NYHHdVl7TUN3MDC9JFAvODF2IN88US=> NG\hUYk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi4NVU3Pyd-MkS4PFE2Pjd:L3G+
FDC-P1 M3XZcWJEVC1{IHnubIljcXSrb36gZZN{[Xl? MVOyOEBp NGHUTHpKSzVyIE2gNE4xOTVizszN NF;1SFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi4NVU3Pyd-MkS4PFE2Pjd:L3G+
NCI-H1963 NULkOIdCS3m2b4TvfIlkcXS7IHHzd4F6 NIPicIE1KGSjeYO= M2DZcmlEPTBiPTCwMlAzPjZizszN MlTxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NEiyPVgoRjJ|NES4Nlk5RC:jPh?=
NCI-H187 MnrkR5l1d3SxeHnjbZR6KGG|c3H5 NHmwWlI1KGSjeYO= Mo\aTWM2OCB;IECuNFM5PCEQvF2= Mk\xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NEiyPVgoRjJ|NES4Nlk5RC:jPh?=
NCI-H1417 NUPlXnV2S3m2b4TvfIlkcXS7IHHzd4F6 NVm5WYJHPCCmYYnz NH7XdlFKSzVyIE2gNE4xPTR{IN88US=> NGDZRXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S0PFI6QCd-MkO0OFgzQTh:L3G+
FDC-P1 NWPtd4F{SkOOLYjMJIlvcGmkaYTpc44h[XO|YYm= NHSzeIMzPCCq MnX0TWM2OCB;IECuNFYh|ryP MkDKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6OEG1OlcoRjJ2OEixOVY4RC:jPh?=
NCI-H146 NFnhdVhEgXSxdH;4bYNqfHliYYPzZZk> MX60PEBp NYq4SVdiTUN3MDC9JFAvODh4NzFOwG0> M4LnTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OESxPFgzLz5zOEi0NVg5OjxxYU6=
RS4:11 M4HhVWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MnXZSWM2OCB;IECuNVEzKM7:TR?= NVriOXVHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5NlYzPDdpPkK4PVI3OjR5PD;hQi=>
MOLT4 M4rFOGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MYTFR|UxKD1iMD6zNFMh|ryP NIGzbFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEmyOlI1Pyd-Mki5NlYzPDd:L3G+
EoL-1-cell MmC4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlTGTWM2OD1yLkCwOlY6KM7:TR?= NGnvPZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
MV-4-11 NWjK[5dtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3KzbGlEPTB;MD6wNVU5PiEQvF2= MmjxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
NKM-1 NXHvZppZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\YVIZOUUN3ME2wMlAyPjl7IN88US=> NIHXeI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
ML-2 NG[wXW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXy2NGNFUUN3ME2wMlAyQTh|IN88US=> NXzEZmpuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
BV-173 MmK2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIOxbVdKSzVyPUCuNFI{OTRizszN MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
RS4-11 NYjGWWNvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnH5TWM2OD1yLkCyOVg4KM7:TR?= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
HL-60 M3\3cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MULJR|UxRTBwMEK5NFgh|ryP MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
KY821 MlvQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUnJR|UxRTBwMEK5O|Uh|ryP NXXVWI4yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
ECC10 M2r1T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml3uTWM2OD1yLkCzO|kzKM7:TR?= MkLtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
NCI-H720 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4\XUWlEPTB;MD6wOFAyOSEQvF2= NYH2PZJvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
QIMR-WIL NUP3eZdNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRTBwMESyPFch|ryP NXr4eXVKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
KG-1 MlftS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWDWdlRpUUN3ME2wMlA1PDh4IN88US=> M1HIe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
TGW M{XMXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkTXTWM2OD1yLkC0OlM{KM7:TR?= NHzqd5g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
ATN-1 NYr6eHZJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVvJR|UxRTBwMES3N|Mh|ryP MnfhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
RH-18 NW\HNXdlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnHNTWM2OD1yLkC2NFQ5KM7:TR?= MorHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
EW-18 M3XqVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYPJR|UxRTBwME[4OFEh|ryP M4q0elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
NB17 M3rOXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYDJR|UxRTBwMEexNlQh|ryP NXf5XZlwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
SK-NEP-1 MlHtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH7tdYFKSzVyPUCuNFczOTNizszN MkDuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
P12-ICHIKAWA NWj1eplRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4PUemlEPTB;MD6wO|c4QCEQvF2= MonKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
KARPAS-45 NXLOWJR3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV:4cZdWUUN3ME2wMlA4QDF3IN88US=> MmjPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
EW-3 MkfZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonzTWM2OD1yLkC4NFU{KM7:TR?= Mon2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
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NCI-H209 NU\FUnJGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYOycJhZUUN3ME2wMlA5PzB2IN88US=> MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
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NH-12 NFzBXWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVT4XY4{UUN3ME2wMlExPzR2IN88US=> MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
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MOLT-4 NFHNdndIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHGwcnBKSzVyPUCuNVUyPjlizszN Ml7FQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
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KP-4 NWLuT3NHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoTsTWM2OD13LkS2N|M1KM7:TR?= MofzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
G-402 NISySXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnHwTWM2OD13LkWxPFY2KM7:TR?= MkTOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
OS-RC-2 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnKyTWM2OD13LkWyOlA1KM7:TR?= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
NCI-H1155 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTVwNUS5OVUh|ryP MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
OE19 MnznS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn24TWM2OD13Lk[4OlI1KM7:TR?= MkfDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
U-2-OS M17nUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVK5OGNTUUN3ME21Mlg6ODF|IN88US=> NWDTfWpMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
SCC-15 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlLITWM2OD13LkmzOlYzKM7:TR?= NUTISnN6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
NCI-H630 NGnQfG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYjJR|UxRTVwOUm0NFQh|ryP M{L3elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
PFSK-1 MnXZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVrJR|UxRTZwMEWyOVkh|ryP NXLzWZlHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
NCI-H1770 MmHuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoTaTWM2OD14LkKwPFc1KM7:TR?= Mk\QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
SK-MEL-3 MlmxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXrS[FRpUUN3ME22MlQzQTF3IN88US=> MkHvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
LB1047-RCC MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3jxOGlEPTB;Nj60O|YzPSEQvF2= NETDO449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
NCI-H446 NXjQbFB7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlHGTWM2OD14Lk[yPVI2KM7:TR?= MkixQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
SW780 MoPPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWHMbJRXUUN3ME22MlcxOTh3IN88US=> NXPEN3pnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
NEC8 M1rFW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGe1WJdKSzVyPU[uO|Y3OyEQvF2= NHywWpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
NOMO-1 M1vHOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF6wc|JKSzVyPU[uO|gyOTFizszN MmPUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
COLO-668 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2O0[mlEPTB;Nj64OFM5PyEQvF2= Ml22QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
MC116 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWe2NVBwUUN3ME22Mlk{QDl5IN88US=> MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
HCC1937 NFToU3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFntTndKSzVyPU[uPVkzPTFizszN MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
NCI-N87 NYrQUoVST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmrxTWM2OD15LkG5Nlk{KM7:TR?= M{GweVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
COLO-320-HSR NEfPd3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEDkUYlKSzVyPUeuNlI4OzhizszN NFvkVoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
HCC1806 NXT5R4ZPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXriNXhwUUN3ME23MlI3ODR2IN88US=> NED4ZpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
OVCAR-3 Mo\0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXrpendNUUN3ME23MlM{ODN6IN88US=> NEHEd|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
NUGC-3 NIPTdppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXf5eZBYUUN3ME23MlM6Pjl2IN88US=> MmPrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
SW1783 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mni4TWM2OD15LkSzNVc2KM7:TR?= NIi3Zng9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
GCT MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3;xNmlEPTB;Nz61OlkxPiEQvF2= NWXy[XZWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
NCI-H2126 NHTmTYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2fSOGlEPTB;Nz63N|YzPSEQvF2= NIXZS3Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
MEL-HO NXzxNm5lT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWPJR|UxRTdwN{ewOVQh|ryP MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
CAPAN-1 NUfKNWhmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHHvN49KSzVyPUeuO|c{PTdizszN NXf1W29lRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
SW756 M3HtOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTdwN{izN|Mh|ryP Mm\NQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
SKG-IIIa MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1qwRWlEPTB;Nz64NVg6OiEQvF2= MnrJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
HCE-T MkjVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUnJR|UxRTdwOEe3PFMh|ryP MlTTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
Ca-Ski M12xNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{TjZWlEPTB;Nz65PVM5OyEQvF2= M2rHelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
COLO-684 MoP3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MojOTWM2OD16LkCxPFE5KM7:TR?= NFvH[FM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
KYSE-70 NHLUW|VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH\JNpVKSzVyPUiuNFc4OjlizszN MnK0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
TI-73 NV3ORY5xT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHvVc2NKSzVyPUiuNlU5PTFizszN M1;PcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
BT-20 NVT3NIFuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFqxWGpKSzVyPUiuNlYxPTJizszN MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
MHH-ES-1 NV\tVJcyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NETtUZRKSzVyPUiuOVE5OzRizszN NWHX[2E5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
TE-12 M4rRfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHLH[5pKSzVyPUiuOVk6OzFizszN MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
YH-13 NYfEVZB5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIr1PJJKSzVyPUiuOlExODhizszN MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
SF126 M1n5TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{XINWlEPTB;OD64N|g3PSEQvF2= MofVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
J82 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NETuPGNKSzVyPUiuPVAxOzhizszN MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
RCC10RGB NX3lRYJGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVWy[XhFUUN3ME24Mlk6PTZzIN88US=> NEjpW2E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,
SK-UT-1 MmDhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXTJR|UxRTlwMES5OFUh|ryP M1e5SlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
LB2241-RCC NHrS[IpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVjJR|UxRTlwMUmxN|ch|ryP MnPiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
LB996-RCC M17RWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkfuTWM2OD17LkG5PFkh|ryP MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OFM3QDRxJ{7TRW5ITVJ:L3G+
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MKN28 NEjrdmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV3rUlNLUUN3ME2zN{4{QDB7IN88US=> NYSwdXk3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
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NCI-H2030 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYDJR|UxRTR5LkKzO|Qh|ryP M2\ZblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
SW948 NYXBbXhxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkWyTWM2OD12Nz60OlQh|ryP NYrwPFJrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
BALL-1 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVTJR|UxRTR5Lk[xOlgh|ryP NVPFZ2N2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
TE-9 MoXDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3r5OmlEPTB;NEeuPVU5OSEQvF2= M4m5U|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
SK-N-FI NFrhbJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYfjXFhVUUN3ME20PE4xOzV6IN88US=> MkHLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQ{Pjh2Lze+V2FPT0WUPD;hQi=>
KALS-1 M3q5UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NETRVWlKSzVyPUS4MlEzQDlizszN NXXZSYQ6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDR|Nki0M{c,W0GQR1XSQE9iRg>?
HO-1-N-1 NUHZe2xtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnzpTWM2OD12OD63OFQ2KM7:TR?= M3vsc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
NCI-H2452 Ml7pS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoT5TWM2OD12OT6xNVUzKM7:TR?= M3jp[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1OzZ6ND:nQnNCVkeHUkyvZV4>
OC-314 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYHEcJFbUUN3ME20PU43QDN2IN88US=> NF\Uc|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PDN4OESvK|5USU6JRWK8M4E,

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
STAT3 / pSTAT3 / STAT6 / pSTAT6 / Mcl-1 / Cleaved Mcl-1 / Bcl-xl / Bim / puma / Bik / Bax / PARP / c-Myc; 

PubMed: 26811457     


(A) Western blot analysis of expressions of STAT3/p-STAT3 and STAT6/p-STAT6 as well as antiapoptotic members (Bcl-2, Bcl-xL, and Mcl-1) after 48 h of treatments in HDLM-2 cells. (B) Western blot analysis of expressions of proapoptotic proteins (Bim, Puma, Bik, Bax, and p-Bad) as well as caspase cascade molecules (PARP and c-Myc) after 48 h of treatments. β-Actin was used as an input control.

caspase3 / cleaved caspase 3; 

PubMed: 24481442     


(b) (i) N/TERT, (ii) SINCE and (iii) SINEG2 cells were either untreated (0 μM/DMSO) or treated with increasing doses of Navitoclax (ABT-263; 0.025–30 μM) and were incubated for 24 h. Next, cells were collected and whole-cell lysates were prepared and immunoblotted against anti-full-length Caspase 3 (∼35 kDa) antibody, whereas β-actin (∼42 kDa) was used as a protein-loading control. Apoptosis was assessed through detecting the cleaved bands of Caspase-3 (∼19, 17 kDa). 

26811457 24481442
Growth inhibition assay
Cell viability; 

PubMed: 25685063     


(C) Hcc827, (D) H292, and (E) H1993 cells were treated with increasing concentrations of albendazole or mebendazole in the presence of navitoclax (0, 1, 2, and 4 μM). Viability was determined after 1 day.

25685063
In vivo When ABT-263 is administered at 100 mg/kg/day in the H345 xenograft model, significant antitumor efficacy is observed with 80% TGI and 20% of treated tumors indicating at least a 50% reduction in tumor volume. [2] Oral administration of ABT-263 alone causes complete tumor regressions in xenograft models of small-cell lung cancer and acute lymphoblastic leukemia. In xenograft models of aggressive B-cell lymphoma and multiple myeloma where ABT-263 displays modest or no single agent activity, it significantly enhances the efficacy of clinically relevant therapeutic regimens. [2]

Protocol

Kinase Assay:

[1]

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Affinity determination:

Binding affinities (Ki or IC50) of ABT-263 against different isoforms of Bcl-2 family are determined with competitive fluorescence polarization assays. The following peptide probe/protein pairs are used: f-bad (1 nM) and Bcl-xL (6 nM), f-Bax (1 nM) and Bcl-2 (10 nM), f-Bax (1 nM) and Bcl-w (40 nM), f-Noxa (2 nM) and Mcl-1 (40 nM), and f-Bax (1 nM) and Bcl-2-A1 (15 nM). Binding affinities for Bcl-xL are also determined using a time-resolved fluorescence resonance energy transfer assay. Bcl-xL (1 nM, His tagged) is mixed with 200 nM f-Bak, 1 nM Tb-labeled anti-His antibody, and ABT-263 at room temperature for 30 min. Fluorescence is measured on an Envision plate reader using a 340/35 nm excitation filter and 520/525 (f-Bak) and 495/510 nm (Tb-labeled anti-His antibody) emission filters.
Cell Research:

[1]

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  • Cell lines: SCLC cell lines
  • Concentrations: 0-1 μM
  • Incubation Time: 48 hours
  • Method:

    Human tumor cell lines SCLC cell lines are maintained at 37℃ containing 5% CO2. SCLC cell lines are cultured in RPMI 1640 with 10% fetal bovine serum (FBS), 1% sodium pyruvate, 25 mM HEPES, 4.5 g/L glucose, and 1% penicillin/streptomycin. Leukemia and lymphoma cell lines are cultured in RPMI 1640 supplemented with 10% FBS and 1% penicillin/streptomycin. Cells (1-5×10 4) are treated by ABT-263 for 48 hours in 96-well culture plates in a final volume of 100 μL and cytotoxicity is assessed with the CellTiter Glo assay. In vitro cyto toxicity of ABT-263 is assayed.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: C.B.-17 scid-bg or C.B.-17 scid mice
  • Dosages: 100 mg/kg/d
  • Administration: Administered via p.o.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (102.6 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+corn oil
For best results, use promptly after mixing.
4mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 974.61
Formula

C47H55ClF3N5O6S3

CAS No. 923564-51-6
Storage powder
in solvent
Synonyms N/A
Smiles CC1(CCC(=C(C1)CN2CCN(CC2)C3=CC=C(C=C3)C(=O)NS(=O)(=O)C4=CC(=C(C=C4)NC(CCN5CCOCC5)CSC6=CC=CC=C6)S(=O)(=O)C(F)(F)F)C7=CC=C(C=C7)Cl)C

In vivo Formulation Calculator (Clear solution)

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Bio Calculators

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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04480086 Active not recruiting Drug: Mivebresib|Drug: Navitoclax|Drug: Ruxolitinib Myelofibrosis (MF) AbbVie March 17 2021 Phase 1
NCT04454658 Recruiting Drug: ABBV-744|Drug: Navitoclax|Drug: Ruxolitinib Myelofibrosis (MF) AbbVie November 11 2020 Phase 1
NCT04041050 Recruiting Drug: Navitoclax|Drug: Ruxolitinib|Drug: Celecoxib Myeloproliferative Neoplasm AbbVie November 8 2019 Phase 1
NCT03181126 Completed Drug: Navitoclax|Drug: Chemotherapy|Drug: Venetoclax Acute Lymphoblastic Leukemia (ALL)|Lymphoblastic Lymphoma AbbVie November 27 2017 Phase 1
NCT02520778 Active not recruiting Drug: Navitoclax|Drug: Osimertinib Advanced Lung Non-Squamous Non-Small Cell Carcinoma|Metastatic Lung Non-Squamous Non-Small Cell Carcinoma|Stage III Lung Non-Small Cell Cancer AJCC v7|Stage IIIA Lung Non-Small Cell Cancer AJCC v7|Stage IIIB Lung Non-Small Cell Cancer AJCC v7|Stage IV Lung Non-Small Cell Cancer AJCC v7 National Cancer Institute (NCI) March 31 2016 Phase 1
NCT02591095 Completed Drug: ABT-263 Platinum-resistant or Refractory Ovarian Cancer Centre Francois Baclesse|ARCAGY/ GINECO GROUP|French Cancer Research Hospital Program January 2016 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I would like to deliver S1001 via i.p. injection, can you suggest a vehicle solution?

  • Answer:

    S1001 ABT-263 (Navitoclax) can be dissolved in 5% DMSO+corn oil at 7mg/ml as a clear solution.

  • Question 2:

    Could you tell me if the catalog number S1001 can go through the hemato-encephalic barrier?

  • Answer:

    According to the paper: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3223694/, ABT-263 can not cross brain-blood barrier.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID