Alda 1

Catalog No.S5800 Batch:S580001

Print

Technical Data

Formula

C15H11Cl2NO3
Molecular Weight 324.16 CAS No. 349438-38-6
Solubility (25°C)* In vitro DMSO 65 mg/mL (200.51 mM)
Ethanol 12 mg/mL warmed with 50ºC water bath (37.01 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Alda 1 is an ALDH2 agonist that activates catalysis for both wild-type ALDH2 (ALDH2*1) and ALDH2*2.
Targets
ALDH2 [1]
In vitro Alda-1 alleviated liver injury after hepatic I/R. Pretreatment with Alda-1 significantly alleviated I/R-induced elevations of alanine aminotransferase and aspartate aminotransferase, and significantly blunted the pathological injury of the liver. Moreover, Alda-1 significantly inhibited ROS and proinflammatory cytokines production, 4-HNE and MDA accumulation, and apoptosis. Increased ALDH2 activity was found after Alda-1 administration. No significant changes in ALDH2 expression were observed after I/R[2].

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    normal rat hepatocyte cell (BRL-3A)

  • Concentrations

    20 μM

  • Incubation Time

    1 hour

  • Method

    The H/R (Hypoxia/reoxygenation) group cells are incubated under a hypoxic atmosphere (95% N2+5% CO2) for 6 h followed by 120 min reoxygenation (95% air + 5% CO2). The control incubation is normoxic conditions (95% air + 5% CO2) for the entire experiment. In the H/R+Alda-1 group, cells are incubated with Alda-1 (20 μM) for 60 min at 37℃ (95% air/5% CO2) before exposure to hypoxia. Furthermore, BRL-3A cells are treated with 5 μM 4-HNE for 60min before exposure to hypoxia to estimate whether 4-HNE could affect H/R.
Animal Study:

[2]
  • Animal Models

    Male Sprague-Dawley rats (IR model)

  • Dosages

    10 mg/kg

  • Administration

    i.p.

Selleck's Alda 1 has been cited by 1 publication

The Effects of Alda-1 Treatment on Renal and Intestinal Injuries After Cardiopulmonary Resuscitation in Pigs [ Front Med (Lausanne), 2022, 9:892472] PubMed: 35646953

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.