Home Biosimilar Antibodies Apoptosis TNF-alpha inhibitor Adalimumab (anti-TNF-alpha)
For research use only. Not for use in humans.

Adalimumab (anti-TNF-alpha)

Synonyms: LU200134, D2E7

Adalimumab (anti-TNF-alpha) is the first fully human, recombinant IgG1 monoclonal antibody that specifically targets human TNF-alpha, MW: 144.19 KD.

Adalimumab (anti-TNF-alpha) Chemical Structure

Click to purchase the isotype control of Adalimumab (anti-TNF-alpha)

15 Citations

The Nobel Prize

17 Nobel Prize winners have used Selleck products

Shimon Sakaguchi

Nature Communications 2025,16, Article number:1325

David Baker

Dev Cell 2023, 58(20):2163-2180.e9.

David Julius

Cell 2017, 185-198.e16

Michael Houghton

Cell Chem Biol, 2020, 27(7):780-792.e5

Charles M. Rice

Cell 2018, 172(3):423-438.e25

Quality Control

Batch: Purity: 99.23% Protein concentration: 5.00 mg/ml Endotoxin Level: ≤1 EU/mg
99.23

Specificity

Name Citation TNF-α TNF-alpha Others
QNZ (EVP4593) 156 NF-κB
JTE-607 Dihydrochloride 0 IL-1beta,IL-8,IL-6
PF-3644022 1 MK2
UCB-9260 0 NF-κB
MD2-TLR4-IN-1 0 TLR4,IL-6
R-7050 8 IL-1β
Resatorvid (TAK-242) 63 TLR4,IL-6,NO
Apigenin-7-O-glucuronide 0
UTL-5g 0
Benpyrine racemate 1
Amarogentin 0 TAS2R1,AMPK
Stylopine 0 IL-1β,NO,COX-2
Myrislignan 1 iNOS,IL-6,NF-κB
NE 52-QQ57 1 iNOS,IL-6,IL-1β
(3R,8S)-Falcarindiol 3 IL-1β,IL-6,iNOS
Gardenoside 0 NF-κB,P2X7 Receptor,IL-6
Cucurbitacin IIb 0 JNK,STAT3,NF-κB
IQ 3 1 IL-6,JNK3,JNK1
Isuzinaxib (APX-115 free base) 0 IL-6,MCP-1/CCL2,NOX2
AUDA 0 Smad3,TGF-β,IL-1β
CPI-1189 1
Madecassic acid 1 IL-1β,COX-2,iNOS
Homoplantaginin 1 IL-6,IKKβ,NF-κB
2',5'-Dihydroxyacetophenone 1 IL-6,NF-κB,ERK1/2
Forsythoside B 0 IκB,NF-κB,IL-6
Mulberroside A 0 ERK,NF-κB,Caspase-1
Ginsenoside Rb1 4 TLR3,NF-κB,IRAK-1
Muscone 4 IL-6,IL-1β,NLRP3 inflammasome
(±)-Shikonin 41 NF-κB,Proteasome,chemokine receptor
Methylthiouracil 0 IL-6,ERK1/2,NF-κB
Expand to Check More
1. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation. 2. "✔" indicates inhibitory effect, but without specific value.

Mechanism of Action

Description
Adalimumab (anti-TNF-alpha) is the first fully human, recombinant IgG1 monoclonal antibody that specifically targets human TNF-alpha, MW: 144.19 KD.
Features
Human IgG1
Targets/IC50/Ki
TNF-α
In vitro

Adalimumab treatment alters the secretion of cytokines.
Cell Research

Fa2N-4 cells were co-cultivated (CC) with monocyte derived macrophages (MDMs) for 24 h. Physiological (15%) and pro-inflammatory (50%) MDM-to-Fa2N-4 ratios were used. All measured cytokine concentrations were related to the corresponding control (fold change) to analyse (A) the IL-8 neutralising antibody (αIL-8, 100 ng/ml) specific effect and (B) the TNF-α neutralising effect of Adalimumab.
In vivo

We injected adalimumab intraductally into the MG of adult mice;this was found to stimulate BSC multipotency.
Animal Research

Animal Models: K5CreER, K8rtTA, TetO-DTA mice
Dosages: 0.15 mg
Administration: i.d.
Reference:[2]
References

Product Details

CAS No. 331731-18-1
Isotype Human IgG1
Source CHO cells
Sterility 0.2 μM filtered
Formulation 100 mM Pro, 20 mM Arg, pH 5.0
Storage
(From the date of receipt)		 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

Comparison of Clones for

*Literature analysis of various clone (for this target) products available on the market shows that Selleck's selected clones are more frequently applied. (data until September 2024)

Tech Support

Handling Instructions

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Frequently Asked Questions

Question 1:
How to store the antibody?

Answer:
Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.