Adalimumab

Catalog No.A2010 Synonyms: LU200134, D2E7 Non-humanized mouse model applicable

For research use only. Not for use in humans.

Adalimumab is the first fully human, recombinant IgG1 monoclonal antibody that specifically targets human TNF-alpha, MW: 144.19 KD.

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Biological Activity

Description Adalimumab is the first fully human, recombinant IgG1 monoclonal antibody that specifically targets human TNF-alpha, MW: 144.19 KD.
Targets
TNF-α [1]
()
In vitro

Adalimumab blocks the interaction of TNF with the p55 and p75 cell surface TNF receptors, thereby neutralising the activity of this cytokine. Through its anti-TNF actions, adalimumab reduces concentrations of matrix metalloproteases (MMP-1 and -3) and other markers of cartilage and synovium turnover, reduces of matrix metalloproteases (MMP-1 and -3) and other markers of cartilage and synovium turnover, and reduces concentrations of acute phase reactants of inflammation (C-reactive protein [CRP] and erythrocyte sedimentation rate [ESR]) and serum cytokines (IL-1β mRNA, IL-1 receptor antagonist, IL-6)[1].

In vivo Adalimumab is safe and well tolerated. In healthy adults, the average absolute bioavailability of adalimumab 40 mg administered subcutaneously (s.c.) is 64%. Concentrations in the synovial fluid are 31-96% of those of the serum. Maximum plasma concentrations occur at 131 (± 56) h, and the mean half-life is ∼ 2 weeks (range, 10-20 days)[1]. Adalimumab treatment attenuates the Ovalbumin(OVA)-induced increase in serum IgE, TH2 and TH1 derived inflammatory cytokines (IL-4 and IFN-γ, respectively) in bronchoalveolar lavage (BAL) fluid, suppresses recruitment of inflammatory cells in BAL fluid and lung, and inhibits BAL fluid neutrophilia. It also ameliorates goblet cell metaplasia and bronchial fibrosis[2].

Protocol

Cell Research:
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  • Objective: Adalimumab induces apoptosis of THP-1 cells
    Cells: THP-1 cells
    Concentrations: 10μg/mL
    Incubation Time: 6, 12, 18, 24, 30 hours
    Method: THP-1 cells (0.5×106) were incubated with anti-FcRI mAb and anti-FcRII mAb, and then cultured with adalimumab, infliximab, huIgG or vehicle (PBS) alone as indicated. LPS (1μg/mL) was added in all culture conditions. Apoptosis was analyzed at different time points by staining with annexin V-FITC and propidium iodide, Apo2.7-PE-conjugated antibody, or 7AAD.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/16374254

    Objective: Effect of TNF-α inhibition with adalimumab on endothelial adhesion molecules
    Cells: HUVECs (Human Umbilical Vein Endothelial cells), THP-1 cells
    Concentrations: 1 μg/ml
    Incubation Time: 6 hours
    Method: HUVECs were incubated with conditioned media from oxLDL-stimulated THP-1 cells (oxLDL CM) with or without adalimumab , and mRNA expression of the adhesion molecules VCAM-1, ICAM-1 and E-selectin in HUVECs was determined by real time PCR.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/27467817

    Adalimumab can apply to mice (eg: CD1 mice, DBA/1 mice), and various in vitro assays (Only for Reference)
Animal Research:
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  • Objective: Adalimumab ameliorates OVA-induced airway inflammation in mice
    Animal Models: CD1 mice
    Formulation: PBS
    Dosages: 1mg/100 g b.wt
    Administration: i.p.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/27262379

    Objective: The distribution of certolizumab pegol, adalimumab, and infliximab in the inflamed paws of mice with collagen-induced arthritis
    Animal Models: DBA/1 mice
    Formulation: PBS
    Dosages: 2 mg/kg
    Administration: i.v.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/19567252

    Adalimumab can apply to mice (eg: CD1 mice, DBA/1 mice), and various in vitro assays (Only for Reference)

Product Details

Formulation PBS buffer, pH 7.2
Isotype Human IgG
Source CHO cells
Storage Store at -80°C and avoid freeze-thaw cycles.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID