Venetoclax (ABT-199, GDC-0199)

Catalog No.S8048

Venetoclax (ABT-199, GDC-0199) Chemical Structure

Molecular Weight(MW): 868.44

Venetoclax (ABT-199, GDC-0199) is a Bcl-2-selective inhibitor with Ki of <0.01 nM in cell-free assays, >4800-fold more selective versus Bcl-xL and Bcl-w, and no activity to Mcl-1. Phase 3.

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In DMSO USD 680 In stock
USD 270 In stock
USD 970 In stock
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5 Customer Reviews

  • (b) MV4-11 cells were treated in vitro with ABT-199 (0.01, 0.1, 1 or 10 μM) with or without BL-8040 (20 μM) for 24 h in 1% FCS RPMI medium. The percentage of dead cells (PI+ cells) was evaluated by FACS. (c) miR-15a and miR-16-1 transfections were performed using Lipofectamine 2000 transfection reagent. At 72 h post-transfection, cells were stained with Annexin-V and PI. FACS plots and numerical data of % cells are shown. The results are expressed as the mean±s.d. and were analyzed using Student’s t-test (*P ⩽0.05 compared with control).

    Leukemia, 2017, 31(11):2336-2346. Venetoclax (ABT-199, GDC-0199) purchased from Selleck.

    MDA-MB-468 cells were treated with BETd-246 (50 nM), BM-1197 (250 nM), ABT-263 (250 nM), ABT-199 (250 nM) or A-1155463 (250 nM) as indicated for 24 h for Annexin V-PI apoptosis analysis. Data are the mean ± SEM (n=3).

    Cancer Res, 2017, 77(9):2476-2487. Venetoclax (ABT-199, GDC-0199) purchased from Selleck.

  • THP-1 cells were treated with cytarabine alone and in combination with ABT-199 for 8 h. Whole cell lysates were extracted and subjected to Western blotting, and probed with anti-γH2AX or -β-actin antibody. Densitometry for γH2AX expression was measured, normalized to β-actin, and graphed as fold change compared to the no drug control. The data are presented as mean ± standard error from at least 3 independent Western blots. * indicates p < 0.05.

    Mol Oncol 2014 10.1016/j.molonc.2014.09.008. Venetoclax (ABT-199, GDC-0199) purchased from Selleck.

    C33A cells were treated with ABC294640 (5 μmol/L), together with/out ABT-737 (200 nM) or GDC-0199 (200 nM), cells were further cultured for indicated time, cell growth (MTT assay, (A) and apoptosis (Histone DNA ELISA assay, (B) were tested.

    Oncotarget, 2017, 9(2):2384-2394. Venetoclax (ABT-199, GDC-0199) purchased from Selleck.

  • CLL cells were incubated with drugs immediately or co-cultured on CD154 stroma overnight and incubated with the indicated concentrations of ABT-199.

    J Biol Chem 2014 289(23), 16190-9. Venetoclax (ABT-199, GDC-0199) purchased from Selleck.

Purity & Quality Control

Choose Selective Bcl-2 Inhibitors

Biological Activity

Description Venetoclax (ABT-199, GDC-0199) is a Bcl-2-selective inhibitor with Ki of <0.01 nM in cell-free assays, >4800-fold more selective versus Bcl-xL and Bcl-w, and no activity to Mcl-1. Phase 3.
Features Re-engineered version of ABT-263 (Navitoclax).
Bcl-2 [1]
(Cell-free assay)
<0.01 nM(Ki)
In vitro

ABT-199 shows less sensitivity to Bcl-xL, Mcl-1 and Bcl-w with Ki of 48 nM, > 444 nM and 245 nM, respectively. ABT-199 potently inhibits FL5.12-Bcl-2 cells, RS4;11 cells with EC50 of 4 nM and 8 nM, while shows low activity against FL5.12-Bcl-xL cells with EC50 of 261 nM. ABT-199 induces a rapid apoptosis in RS4;11 cells with cytochrome c release, caspase activation, the externalization of phosphatidylserine and the accumulation of sub-G0/G1 DNA. Quantitative immunoblotting reveals that sensitivity to ABT-199 correlated strongly with the expression of Bcl-2, including NHL, DLBCL, MCL, AML and ALL cell lines. ABT-199 also induces apoptosis in CLL with an average EC50 of 3.0 nM. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CS-THL1 M4Tmdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWXBPVR[OjBibl2= M4S3fFczKGh? M1j4emROW09? MWPJcohq[mm2czDj[YxtKGe{b4f0bEBie3Onc4Pl[EBjgSClZXzsJJZq[WKrbHn0fS=> MnHtNlU6OTZ4OUi=
CS-THL1 NIq5cYNCeG:ydH;0bYMhSXO|YYm= NFi5ZWYzPSCwTR?= NXfUXY5sTE2VTx?= NIe2SpVKdmS3Y3XzJIFxd3C2b4Ppdy=> NGfJeFYzPTlzNk[5PC=>
DoGKiT M{Pp[mFxd3C2b4TpZ{BCe3OjeR?= MWS1NEBvVQ>? MlfuSG1UVw>? NVK1dYpzUW6mdXPld{BieG:ydH;zbZM> M1zXO|I2QTF4Nkm4
RS4-11 M{D5Xmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUC2TXNsPzJiaB?= NUXuWWtHUUN3ME2wMlA1ODJizszN MX[yOVY1QTd4OB?=
NALM-6 M4HW[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVu3NkBp MVnJR|UxRjNizszN MlXrNlU3PDl5Nki=
SU-DHL-6 M{DTVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1q4NVAvQCEQvF2= MmTwTY5pcWKrdIOgZ4VtdCCpcn;3eIgh[XO|ZYPz[YQh[nliY3XscEB3cWGkaXzpeJk> NGO1bZIzPTV7MEiwNy=>
OCI-Ly19 NXLT[2k1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUO2Rpp2OSEQvF2= MXXJcohq[mm2czDj[YxtKGe{b4f0bEBie3Onc4Pl[EBjgSClZXzsJJZq[WKrbHn0fS=> Mom3NlU2QTB6MEO=
SU-DHL-6 NGPXZXBHfW6ldHnvckBCe3OjeR?= M1\IcVAvPzVizszN NYjPXZFCOThiaB?= M3nLNWlv[3KnYYPld{Bxem9vc4Xyeol3[WxicILveIVqdiCPQ1ytNUBmgHC{ZYPzbY9v NXLZRmliOjV3OUC4NFM>
KCL22 MUjGeY5kfGmxbjDBd5NigQ>? M1nUfFIh|ryP NE\tWVI1QCCq MmDYSG1UVw>? MWjJcoNz\WG|ZYOgSG5CKG[{YXfhcYVvfGG2aX;u NX64NlV4OjV|M{OyOVI>
LOUCY MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV;4c3g2OTBizszN Ml:5OFghcA>? Ml25SG1UVw>? NUG5dnBlUUN3ME2wMlAyOzlizszN Mmj0NlU{ODF5MES=
ALL-SIL M4njW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEDRcVkyOCEQvF2= M2\MWlQ5KGh? Mon4SG1UVw>? MVzJR|UxRTBwMUiwN{DPxE1? MXmyOVMxOTdyNB?=
CUTLL1 NVrEWnNoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUTqS|BQOTBizszN MnniOFghcA>? NXn5PGpvTE2VTx?= NGr4coxKSzVyPUCuN|gzOyEQvF2= NUjDOohLOjV|MEG3NFQ>
KOPTK1 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3ricVExKM7:TR?= NGn4d4s1QCCq M{e5S2ROW09? NVjC[ZFPUUN3ME2wMlY1OzJizszN M33CUFI2OzBzN{C0
DND-41 M3HjVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4mwUFExKM7:TR?= M4PJflQ5KGh? MWTEUXNQ NYD3SpY1UUN3ME2xMlk3QTVizszN NHHI[ZEzPTNyMUewOC=>
PF-382 M4\jeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{e0VlExKM7:TR?= MnTqOFghcA>? Ml\aSG1UVw>? MWPJR|UxRTJwMUiyOEDPxE1? MX:yOVMxOTdyNB?=
KARPAS-45 M2noU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MojCNVAh|ryP M4Xie|Q5KGh? M3P0[mROW09? MWXJR|UxRTNwMkKyOUDPxE1? M1\QV|I2OzBzN{C0
PEER MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmTUNVAh|ryP M3LUNVQ5KGh? NYjlSot[TE2VTx?= M2TGc2lEPTB;ND62OFA{KM7:TR?= MVuyOVMxOTdyNB?=
CX-1 NG\GU5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYWxNFAh|ryP NWHQcGN4PzJiaB?= M3TYbmlEPTB;Nj63JO69VQ>? NH[2UlgzPTJyOEi4Ni=>
LS147T NEfXbmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVnRU|RkOTByIN88US=> NHPMVFQ4OiCq MYrJR|UxRTJ7LkWg{txO Ml\nNlUzODh6OEK=
HL-60 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFLyXFE1QCCq MV;JR|UxRDFizszN MYiyOFM1PjFzNh?=
MOLM-13 M{fvNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVi0PEBp MkHBTWM2ODxzIN88US=> NG\3T4ozPDN2NkGxOi=>
OCI-AML2 NFrWRZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWq0PEBp NVnjSXBnUUN3MEyxJO69VQ>? NFTab3IzPDN2NkGxOi=>
Kasumi-1 M3PFfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1X3VlQ5KGh? MoexTWM2ODxzIN88US=> MYGyOFM1PjFzNh?=
KG-1 NV22OJBlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUHZWIZ{PDhiaB?= NEP6Z4lKSzVyPEGg{txO MVqyOFM1PjFzNh?=
THP-1 NV\nU5pnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUW0PEBp MnjyTWM2ODxzIN88US=> NXfhdWlYOjR|NE[xNVY>
MOLM-14 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmXrOFghcA>? NEKxVVVKSzVyPEGg{txO MkfZNlQ{PDZzMU[=
MOLM-13 NEHRe2xCeG:ydH;0bYMhSXO|YYm= NEPNNVg2OCCwTR?= Ml[yNlQhcA>? NF7NOVRCeG:ydH;zbZMhcW6mdXP0bY9v NYPnbWllOjR|NE[xNVY>
SKW-3/KE-37 M3S3N2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWWxNEDPxE1? MlT3OFghcA>? MofSSG1UVw>? M4HyeGlEPTB;MD63NVIh|ryP NX3UT3BKOjR|NEK5OFg>
SUPT-11 NH\OOZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2HiSFExKM7:TR?= MWG0PEBp MlziSG1UVw>? MVvJR|UxRTRwNEezJO69VQ>? NVHObmd[OjR|NEK5OFg>
JURKAT MlrYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1vxZ|ExKM7:TR?= NIHWbGM1QCCq Mm\CSG1UVw>? M4XSN2lEPTB;ND64PVMh|ryP NWe2d3RnOjR|NEK5OFg>

... Click to View More Cell Line Experimental Data

In vivo ABT-199 (100 mg/kg) causes a maximal tumor growth inhibition of 95% and tumor growth delay of 152% in RS4;11 xenografts. ABT-199 also inhibits xenograft growth (DoHH2, Granta-519) as a single agent or in combination with SDX-105 and other agents. [1]


Kinase Assay:[1]
+ Expand

Binding affinity assays:

Binding affinities (Ki or IC50) of ABT-199 against different isoforms of Bcl-2 family are determined with competitive fluorescence polarization assays. The following peptide probe/protein pairs are used: f-bad (1 nM) and Bcl-xL (6 nM), f-Bax (1 nM) and Bcl-2 (10 nM), f-Bax (1 nM) and Bcl-w (40 nM), f-Noxa (2 nM) and Mcl-1 (40 nM), and f-Bax (1 nM) and Bcl-2-A1 (15 nM). Binding affinities for Bcl-xL are also determined using a time-resolved fluorescence resonance energy transfer assay. Bcl-xL (1 nM, His tagged) is mixed with 200 nM f-Bak, 1 nM Tb-labeled anti-His antibody, and ABT-199 at room temperature for 30 min. Fluorescence is measured on an Envision plate reader using a 340/35 nm excitation filter and 520/525 (f-Bak) and 495/510 nm (Tb-labeled anti-His antibody) emission filters.
Cell Research:[1]
+ Expand
  • Cell lines: NHL, DLBCL, MCL, AML and ALL cell lines
  • Concentrations: ~1 μM
  • Incubation Time: 48 hours
  • Method: RS4;11 cells are seeded at 5 × 104 per well in 96-well plates and treated with ABT-199 diluted in half-log steps starting at 1 μM-0.05 nM. Leukemia and lymphoma cell lines are seeded at 1.5-2 × 104 cells per well in the appropriate medium and incubated with ABT-199 for 48 h. Effects on proliferation are determined using Cell TiterGlo reagent. EC50 values are determined by nonlinear regression analysis of the concentration-response data.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: Female C.B-17 SCID mice (DoHH2 and Granta-519 xenografts) and female C.B-17 SCID-beige mice (RS4;11 and Toledo xenografts)
  • Formulation: 60% phosal 50 propylene glycol (PG), 30% polyethylene glycol (PEG) 400 and 10% ethanol
  • Dosages: ~100 mg/kg
  • Administration: Orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (115.14 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+50% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 868.44


CAS No. 1257044-40-8
Storage powder
in solvent
Synonyms N/A

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03735875 Not yet recruiting Acute Myeloid Leukemia With FLT3/ITD Mutation|FLT3 Gene Mutation|FLT3 Internal Tandem Duplication|Recurrent Acute Myeloid Leukemia|Refractory Acute Leukemia M.D. Anderson Cancer Center|National Cancer Institute (NCI) March 31 2019 Phase 1|Phase 2
NCT03708003 Not yet recruiting Relapsed/Refractory Chronic Lymphocytic Leukemia|Chronic Lymphocytic Leukemia|Leukemia Swiss Group for Clinical Cancer Research March 2019 Phase 2
NCT03710772 Not yet recruiting CD20 Positive|Mantle Cell Lymphoma M.D. Anderson Cancer Center|National Cancer Institute (NCI) January 31 2019 Phase 2
NCT03701321 Not yet recruiting Recurrent Plasma Cell Myeloma|Refractory Plasma Cell Myeloma National Cancer Institute (NCI) January 25 2019 Phase 1|Phase 2
NCT03639324 Not yet recruiting Chronic Lymphocytic Leukemia|CLL|Relapsed CLL|Refractory Chronic Lymphocytic Leukemia|Relapsed Chronic Lymphocytic Leukemia Virginia Commonwealth University January 31 2019 Phase 1
NCT03573024 Not yet recruiting Acute Myeloid Leukemia University of Colorado Denver January 2019 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    Could you please offer some advice on the half-life of the drug ?

  • Answer:

    According to the reference (, the half-life of ABT-199 in dogs is 12.9 hr.

  • Question 2:

    how to prepare the working solution for mice including how to dissolve the powder?

  • Answer:

    We recommend the following vehicle for ABT 199, 30% PEG400/0.5% Tween80/5% Propylene glycol (64.5% water, V/V), at a concentration up to 20mg/ml. Its a homogeneous suspension and can be used for oral gavage.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID