Nicotinamide (NSC 13128)

For research use only.

Catalog No.S1899 Synonyms: Niacinamide, Vitamin PP, Nicotinic acid amide, Vitamin B3, NSC 27452

21 publications

Nicotinamide (NSC 13128) Chemical Structure

CAS No. 98-92-0

Nicotinamide (NSC 13128, Niacinamide, Vitamin PP, Nicotinic acid amide, Vitamin B3, NSC 27452), a water-soluble vitamin, is an active component of coenzymes NAD and NADP, and also act as an inhibitor of sirtuins.

Selleck's Nicotinamide (NSC 13128) has been cited by 21 publications

Purity & Quality Control

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Biological Activity

Description Nicotinamide (NSC 13128, Niacinamide, Vitamin PP, Nicotinic acid amide, Vitamin B3, NSC 27452), a water-soluble vitamin, is an active component of coenzymes NAD and NADP, and also act as an inhibitor of sirtuins.
Targets
Sirtuin [1]
In vitro

Nicotinamide strongly inhibits yeast silencing, increases rDNA recombination, and shortens replicative life span to that of a sir2 mutant. Nicotinamide abolishes silencing and leads to an eventual delocalization of Sir2 even in G(1)-arrested cells, demonstrating that silent heterochromatin requires continual Sir2 activity. [1] Nicotinamide results in a twofold increase in DNA content and a threefold increase in insulin content in the fetal cells. Nicotinamide induces differentiation and maturation of human fetal pancreatic islet cells. [2] Nicotinamide regulates sirtuins by switching between deacetylation and base exchange. Nicotinamide switching is quantitated for the Sir2s from Archeaglobus fulgidus (Sir2Af2), Saccharomyces cerevisiae (Sir2p), and mouse (Sir2alpha). [3] Nicotinamide selectively reduces a specific phospho-species of tau (Thr231) that is associated with microtubule depolymerization in Alzheimer's disease transgenic mice, in a manner similar to inhibition of SirT1. Nicotinamide also dramatically increases acetylated alpha-tubulin, a primary substrate of SirT2, and MAP2c in Alzheimer's disease transgenic mice, both of which are linked to increased microtubule stability. [4] Nicotinamide fosters DNA integrity and maintains phosphatidylserine membrane asymmetry to prevent cellular inflammation, cellular phagocytosis and vascular thrombosis. Nicotinamide both prevents and reverses neuronal and vascular cell injury. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
BL21 (DE3) MYHGeY5kfGmxbjDhd5NigQ>? NU[xOm1YUW6qaXLpeIlwdiCxZjDjZZRidHm2aXPhcIx6KGGldHn2[UBpfW2jbjDTTXJVOyBqMUCyJJRwKDN7OTDhcYlvdyCjY3nkd{kh\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliQlyyNUApTEV|KTDj[YxteyC3c3nu[{BndHWxcn;n[Y5q[yB5LXHtbY5wNTRvbXX0bJlt[2:3bXHybY4hMEGPQzmtcIFj\WynZDDw[ZB1cWSnIHL5JIZtfW:{ZYPj[Y5k\SCjc4PhfUwhUUN3ME22MlLPxE1w MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ5NUiyOEc,OjV{N{W4NlQ9N2F-
BL21 (DE3) NETwO5dHfW6ldHnvckBie3OjeR?= M3n1fGlvcGmkaYTpc44hd2ZiZoXscEBt\W6pdHigbJVu[W5iU1nSWFIh\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliQlyyNUApTEV|KTDj[YxteyC3c3nu[{BndHWxcn;n[Y5q[yB5LXHtbY5wNTRvbXX0bJlt[2:3bXHybY4hMEGPQzmtcIFj\WynZDDw[ZB1cWSnIHL5JIZtfW:{ZYPj[Y5k\SCjc4PhfUwhUUN3ME2xNE42|ryPLh?= M4TufVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Mke1PFI1Lz5{NUK3OVgzPDxxYU6=
BL21(DE3) MlfzSpVv[3Srb36gZZN{[Xl? MX3Jcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IGPjbIl{fG:|b33hJI1idnOxbnmgd4lzfHWrbjCyJEgzOSC2bzCzNlIhemW|aXT1[ZMqKGW6cILld5Nm\CCrbjDFd4Np\XKrY3jpZUBkd2yrIFLMNlEpTEV|KTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHO3YoP0doF1\SCmZXHj[ZR6dGG2aX;uJJV{cW6pIGrNRWwh[XNic4Xid5Rz[XSnLDDJR|UxRTJ|LkJOwG0v Mk\mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF2OU[yOVEoRjNzNEm2NlUyRC:jPh?=
SK-MEL-28 NY\YTZJFTnWwY4Tpc44h[XO|YYm= MoHENVAxKHWP NWLyWm57OjRiaILz MUPS[YR2[3Srb36gbY4hSVSSIHzleoVtKGmwIHj1cYFvKFONLV3FUE0zQCClZXzsd{BifCBzMECgeW0hdWGrboThbY5m\CCrbjDMc4Ns\Se|IIPvcJV1cW:wIHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnLXLhd4VlKGG|c3H5JIlvKGGkc3XuZ4Uhd2ZiR1[gZY5lKGeudXPvd4U> MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh|NUexPUc,OjJ6M{W3NVk9N2F-
A673 NHzJeIRyUFSVIHHzd4F6 MUXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDBOlc{KGOnbHzzLS=> M4XVclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-MC NEHKWGFyUFSVIHHzd4F6 M1LRfpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHNMNU5vTVOgZ4VtdHN? MnT3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB-EBc1 NVfRcmJjeUiWUzDhd5NigQ>? M1jW[ZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJG5DNUWEY{GgZ4VtdHN? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
TC32 NUXGOpJxeUiWUzDhd5NigQ>? MUHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDUR|MzKGOnbHzz MnvWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
BL21(DE3) MkjjSpVv[3Srb36gZZN{[Xl? NXX5NohYUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBU[2irc4Tvd49u[SCvYX7zc45qKHOrcoT1bY4hOiBqMkGgeI8hOzJ{IILld4llfWW|KTDlfJBz\XO|ZXSgbY4hTXOlaHXybYNpcWFiY3;sbUBDVDJzKFTFN{kh[2WubIOgbY4heHKnc3XuZ4Uhd2ZiWl3BUC=> NF;GVWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUS5OlI2OSd-M{G0PVYzPTF:L3G+
BL21(DE3) M3zMNWZ2dmO2aX;uJIF{e2G7 MV;Jcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IGPjbIl{fG:|b33hJI1idnOxbnmgd4lzfHWrbjCyJEgzOSC2bzCzNlIhemW|aXT1[ZMqKGW6cILld5Nm\CCrbjDFd4Np\XKrY3jpZUBkd2yrIFLMNlEpTEV|KTDj[YxteyCrbjDwdoV{\W6lZTDv[kApYilvKFL1eElNgXNvQV3D MonKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF2OU[yOVEoRjNzNEm2NlUyRC:jPh?=
BL21(DE3) NU\ONYNUTnWwY4Tpc44h[XO|YYm= NXLKbHE1UW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBU[2irc4Tvd49u[SCvYX7zc45qKHOrcoT1bY4hOiBqMkGgeI8hOzJ{IILld4llfWW|KTDlfJBz\XO|ZXSgbY4hTXOlaHXybYNpcWFiY3;sbUBDVDJzKFTFN{kh[2WubIOgbY4heHKnc3XuZ4Uhd2ZiKGqpMUhJ\XhrTInzMWFOSw>? NF\wbpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUS5OlI2OSd-M{G0PVYzPTF:L3G+
BL21(DE3) NW\OXHlrTnWwY4Tpc44h[XO|YYm= NWfrN5Z[UW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBU[2irc4Tvd49u[SCvYX7zc45qKHOrcoT1bY4hOiBqMkGgeI8hOzJ{IILld4llfWW|KTDlfJBz\XO|ZXSgbY4hTXOlaHXybYNpcWFiY3;sbUBDVDJzKFTFN{kh[2WubIOgbY4heHKnc3XuZ4Uhd2ZiKGqpMUhQ[3RrTInzMWFOSw>? Ml7mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF2OU[yOVEoRjNzNEm2NlUyRC:jPh?=
HEK293T NIjqTHdHfW6ldHnvckBie3OjeR?= MlTZOlAhdWmwcx?= NHW3e4JKdmirYnn0bY9vKG:oIGP0doVxXGGpLYTh[4dm\CCqdX3hckBUSVKPMTDUTXIhMDV4MTD0c{A4OjRicnXzbYR2\XNrIHX4dJJme3OnZDDpckBJTUt{OUPUJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCQQVThd4Uh[WO2aY\peJkhcW5icILld4Vv[2Vib3[gUmFFMyCrbnP1ZoF1\WRiZn;yJFYxKG2rboOgZpkhUFCOQzDhcoFtgXOrczygTWM2OD12Mz64{txONg>? NIr5e5A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTRyLze+R4hGVUKOPD;hQi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Sp1 / ERK ; 

PubMed: 18446063     


Cells were either mock-treated or treated with nicotinamide at the concentration either of 10 or 20 mM, collected at the indicated time points, and applied to Western blotting analysis.

p-β-catenin / β-catenin ; 

PubMed: 30503259     


The phosphorylation of β-catenin was decreased by 10 mM nicotinamide or 5 μM CK1 inhibitor D4476 treatment for 6 hr (n = 3) as shown by western blot.

p-MLC / MLC / p-MYPT1 / MYPT1 ; 

PubMed: 30503259     


Dose-dependent effect of nicotinamide on the phosphorylation of MYPT1 (Thr 696) and MLC (Ser 19). Individualized hESCs were treated with nicotinamide at indicated concentrations for 1 hr. Top, western blot image. Bottom, quantification of the western blot results (n = 3).

18446063 30503259
Immunofluorescence
p-MLC; 

PubMed: 30503259     


Confocal images of individualized hESCs treated with 10 mM nicotinamide (Nam) or 10 μM ROCK inhibitor Y27632 (ROCKi). Red, phalloidin 594; green, p-MLC (Ser19). Scale bar, 10 μm.

30503259
In vivo In the mouse, nicotinamide given i.p. at doses of 100-500 mg/kg showed biphasic elimination with dose-dependent changes in half-life. The initial half-life increased significantly (P <0.05) from 0.8 to 2 h and the terminal half-life increased from 3.4 to 5.6 h over the dose range studied. Clearance, however, decreased significantly from 0.3 to 0.24 L/kg/h only at the highest dose. Peak concentrations increased in a dose-dependent manner from 1,000 to 4,800 nmol/ml. The bioavailability given via the i.p. as compared with the i. v. route was close to 100%[6].

Protocol

Cell Research:

[7]

- Collapse
  • Cell lines: HaCaT cells
  • Concentrations: 33 μM
  • Incubation Time: 7 days and 14 days
  • Method:

    The established cell line of human epidermal keratinocytes (HaCaT cells) was routinely cultured in Dulbecco's modified Eagle medium (DMEM) containing 10% fetal bovine serum and kept in a humidified atmosphere containing 5% CO2 at 37°C. For NAD(P) modulation, cells were grown in DMEM and 10% dialyzed fetal bovine serum and with addition of 33 μM nicotinamide (33 μM Nam) or without added nicotinamide (0 μM Nam). Cell number was measured by counting. Sensitivity to glutaminase inhibition was performed using 0.1 μM 6-diazo-5-oxo-L-norleucine (DON) on cells grown in 33 μM Nam or 0 μM Nam for 7 days.


    (Only for Reference)
Animal Research:

[6]

- Collapse
  • Animal Models: Mice (strain CBA/Ht/GyfBSVS)
  • Dosages: 100, 200, 300 and 500 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 24 mg/mL (196.52 mM)
Water 24 mg/mL (196.52 mM)
Ethanol '24 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 122.12
Formula

C6H6N2O

CAS No. 98-92-0
Storage powder
in solvent
Synonyms Niacinamide, Vitamin PP, Nicotinic acid amide, Vitamin B3, NSC 27452
Smiles C1=CC(=CN=C1)C(=O)N

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04763499 Not yet recruiting Other: freeze dried strawberry powder Vascular Dilation University of California Davis October 1 2021 Not Applicable
NCT04844528 Recruiting Drug: Nicotinamide|Drug: Placebo Chronic Lymphocytic Leukemia University of Utah August 5 2021 Phase 2
NCT04641663 Recruiting Dietary Supplement: Multi-target Dietary Supplement (MTDS) Aging|Age-related Cognitive Decline Douglas Boreham|McMaster University|Northern Ontario School of Medicine July 15 2021 Not Applicable
NCT04909489 Recruiting Other: cross-sectional study without intervention Traditional Chinese Medicine|Dyslipidemias|Syndrome Dongzhimen Hospital Beijing April 21 2021 --
NCT04342975 Recruiting Drug: Nicotinamide Riboside + Pterostilbene|Drug: Placebo AKI Mayo Clinic|Elysium Health December 1 2020 Phase 2
NCT04387201 Recruiting Drug: Dulaglutide|Drug: Cyanocobalamin Glucose Intolerance|Overweight and Obesity|Drug Effect|Adiposity The University of Texas Health Science Center Houston May 15 2020 Phase 4

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Sirtuin Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID