Nicotinamide (Vitamin B3)
For research use only.
Catalog No.S1899 Synonyms: Niacinamide, Vitamin PP, Nicotinic acid amide
16 publications

CAS No. 98-92-0
Nicotinamide (Vitamin B3, Niacinamide, Vitamin PP, Nicotinic acid amide), a water-soluble vitamin, is an active component of coenzymes NAD and NADP, and also act as an inhibitor of sirtuins.
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Immunoprecipitation-western blot for analyzing acetylated Snail in Snail-transfected 293T cells (upper) and FaDu-Snail cells (lower) treated with a vehicle control (Ctrl), a HDAC inhibitor TSA 5 nM for 8 hr, a SIRT1 inhibitor NAM(Nicotinamide) 10 mM for 8 hr, or in combination.
Cancer Cell,2014, 26(4):534-48.. Nicotinamide (Vitamin B3) purchased from Selleck.
Purity & Quality Control
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Biological Activity
Description | Nicotinamide (Vitamin B3, Niacinamide, Vitamin PP, Nicotinic acid amide), a water-soluble vitamin, is an active component of coenzymes NAD and NADP, and also act as an inhibitor of sirtuins. | |||||||||||||||||||||||
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Targets |
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In vitro |
Nicotinamide strongly inhibits yeast silencing, increases rDNA recombination, and shortens replicative life span to that of a sir2 mutant. Nicotinamide abolishes silencing and leads to an eventual delocalization of Sir2 even in G(1)-arrested cells, demonstrating that silent heterochromatin requires continual Sir2 activity. [1] Nicotinamide results in a twofold increase in DNA content and a threefold increase in insulin content in the fetal cells. Nicotinamide induces differentiation and maturation of human fetal pancreatic islet cells. [2] Nicotinamide regulates sirtuins by switching between deacetylation and base exchange. Nicotinamide switching is quantitated for the Sir2s from Archeaglobus fulgidus (Sir2Af2), Saccharomyces cerevisiae (Sir2p), and mouse (Sir2alpha). [3] Nicotinamide selectively reduces a specific phospho-species of tau (Thr231) that is associated with microtubule depolymerization in Alzheimer's disease transgenic mice, in a manner similar to inhibition of SirT1. Nicotinamide also dramatically increases acetylated alpha-tubulin, a primary substrate of SirT2, and MAP2c in Alzheimer's disease transgenic mice, both of which are linked to increased microtubule stability. [4] Nicotinamide fosters DNA integrity and maintains phosphatidylserine membrane asymmetry to prevent cellular inflammation, cellular phagocytosis and vascular thrombosis. Nicotinamide both prevents and reverses neuronal and vascular cell injury. [5] |
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Cell Data |
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Assay |
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In vivo | In the mouse, nicotinamide given i.p. at doses of 100-500 mg/kg showed biphasic elimination with dose-dependent changes in half-life. The initial half-life increased significantly (P <0.05) from 0.8 to 2 h and the terminal half-life increased from 3.4 to 5.6 h over the dose range studied. Clearance, however, decreased significantly from 0.3 to 0.24 L/kg/h only at the highest dose. Peak concentrations increased in a dose-dependent manner from 1,000 to 4,800 nmol/ml. The bioavailability given via the i.p. as compared with the i. v. route was close to 100%[6]. |
Protocol
Cell Research: |
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Animal Research: |
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Solubility (25°C)
In vitro | DMSO | 24 mg/mL (196.52 mM) |
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Water | 24 mg/mL (196.52 mM) | |
Ethanol | 24 mg/mL (196.52 mM) |
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Information
Molecular Weight | 122.12 |
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Formula | C6H6N2O |
CAS No. | 98-92-0 |
Storage |
powder in solvent |
Synonyms | Niacinamide, Vitamin PP, Nicotinic acid amide |
Smiles | C1=CC(=CN=C1)C(=O)N |
In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment) | ||||||||||
Dosage | mg/kg | Average weight of animals | g | Dosing volume per animal | ul | Number of animals | ||||
Step 2: Enter the in vivo formulation () | ||||||||||
% DMSO % % Tween 80 % ddH2O | ||||||||||
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: : mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL,)
Method for preparing in vivo formulation:Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80,mix and clarify, next add μL ddH2O,mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
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Clinical Trial Information
NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
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NCT04641663 | Not yet recruiting | Dietary Supplement: Multi-target Dietary Supplement (MTDS) | Aging|Age-related Cognitive Decline | Douglas Boreham|McMaster University|Northern Ontario School of Medicine | January 4 2021 | Not Applicable |
NCT04387201 | Recruiting | Drug: Dulaglutide|Drug: Cyanocobalamin | Glucose Intolerance|Overweight and Obesity|Drug Effect|Adiposity | The University of Texas Health Science Center Houston | November 18 2020 | Phase 4 |
NCT04342975 | Recruiting | Drug: Nicotinamide Riboside + Pterostilbene|Drug: Placebo | AKI | Mayo Clinic|Elysium Health | October 30 2020 | Phase 2 |
NCT04110028 | Recruiting | Drug: Nicotinamide riboside|Drug: Placebo | Inflammation|Acute Illness | Oslo University Hospital|ChromaDex Inc. | October 1 2019 | Phase 1|Phase 2 |
NCT03975777 | Recruiting | Other: LO|Other: HI | Mitochondrial Biogenesis | Brendon Gurd PhD|Queen''s University | June 1 2019 | Not Applicable |
NCT03870035 | Not yet recruiting | -- | PCOS | Assiut University | May 1 2019 | -- |
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