Nicotinamide (NSC 13128)

Catalog No.S1899 Synonyms: Niacinamide, Vitamin PP, Nicotinic acid amide, Vitamin B3, NSC 27452

For research use only.

Nicotinamide (NSC 13128, Niacinamide, Vitamin PP, Nicotinic acid amide, Vitamin B3, NSC 27452), a water-soluble vitamin, is an active component of coenzymes NAD and NADP, and also act as an inhibitor of sirtuins.

Nicotinamide (NSC 13128) Chemical Structure

CAS No. 98-92-0

Selleck's Nicotinamide (NSC 13128) has been cited by 25 publications

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Biological Activity

Description Nicotinamide (NSC 13128, Niacinamide, Vitamin PP, Nicotinic acid amide, Vitamin B3, NSC 27452), a water-soluble vitamin, is an active component of coenzymes NAD and NADP, and also act as an inhibitor of sirtuins.
Targets
Sirtuin [1]
In vitro

Nicotinamide strongly inhibits yeast silencing, increases rDNA recombination, and shortens replicative life span to that of a sir2 mutant. Nicotinamide abolishes silencing and leads to an eventual delocalization of Sir2 even in G(1)-arrested cells, demonstrating that silent heterochromatin requires continual Sir2 activity. [1] Nicotinamide results in a twofold increase in DNA content and a threefold increase in insulin content in the fetal cells. Nicotinamide induces differentiation and maturation of human fetal pancreatic islet cells. [2] Nicotinamide regulates sirtuins by switching between deacetylation and base exchange. Nicotinamide switching is quantitated for the Sir2s from Archeaglobus fulgidus (Sir2Af2), Saccharomyces cerevisiae (Sir2p), and mouse (Sir2alpha). [3] Nicotinamide selectively reduces a specific phospho-species of tau (Thr231) that is associated with microtubule depolymerization in Alzheimer's disease transgenic mice, in a manner similar to inhibition of SirT1. Nicotinamide also dramatically increases acetylated alpha-tubulin, a primary substrate of SirT2, and MAP2c in Alzheimer's disease transgenic mice, both of which are linked to increased microtubule stability. [4] Nicotinamide fosters DNA integrity and maintains phosphatidylserine membrane asymmetry to prevent cellular inflammation, cellular phagocytosis and vascular thrombosis. Nicotinamide both prevents and reverses neuronal and vascular cell injury. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
BL21 (DE3) NUHtR5E4TnWwY4Tpc44h[XO|YYm= NEO4RWFKdmirYnn0bY9vKG:oIHPheIFtgXSrY3HscJkh[WO2aY\lJIh2dWGwIGPJVnQ{KChzMEKgeI8hOzl7IHHtbY5wKGGlaXTzLUBmgHC{ZYPz[YQhcW5iRYPjbIVzcWOqaXGgZ49tcSCETEKxJEhFTTNrIHPlcIx{KHW|aX7nJIZtfW:{b3flcolkKDdvYX3pco8uPC2vZYTofYxkd3WvYYLpckApSU2FKT3sZYJmdGWmIIDldJRq\GViYomg[ox2d3Knc3PlcoNmKGG|c3H5MEBKSzVyPU[uNu69VS5? NH\1VVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK3OVgzPCd-MkWyO|U5OjR:L3G+
BL21 (DE3) NEf0O5dHfW6ldHnvckBie3OjeR?= MnjOTY5pcWKrdHnvckBw\iCodXzsJIxmdme2aDDoeY1idiCVSWLUNkBmgHC{ZYPz[YQhcW5iRYPjbIVzcWOqaXGgZ49tcSCETEKxJEhFTTNrIHPlcIx{KHW|aX7nJIZtfW:{b3flcolkKDdvYX3pco8uPC2vZYTofYxkd3WvYYLpckApSU2FKT3sZYJmdGWmIIDldJRq\GViYomg[ox2d3Knc3PlcoNmKGG|c3H5MEBKSzVyPUGwMlXPxE1w MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ5NUiyOEc,OjV{N{W4NlQ9N2F-
BL21(DE3) NWmzW21kTnWwY4Tpc44h[XO|YYm= M3jiRmlvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgV4NpcXO2b4PvcYEhdWGwc3;ubUB{cXK2dXnuJFIhMDJzIITvJFMzOiC{ZYPp[JVmeyliZYjwdoV{e2WmIHnuJGV{[2incnnjbIliKGOxbHmgRmwzOSiGRUOpJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2Zic4Xid5Rz[XSnIHTlZYNmfHmuYYTpc44hfXOrbnegXm1CVCCjczDzeYJ{fHKjdHWsJGlEPTB;MkOuNe69VS5? MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTR7NkK1NUc,OzF2OU[yOVE9N2F-
SK-MEL-28 MlLSSpVv[3Srb36gZZN{[Xl? M1jjZ|ExOCC3TR?= NYjpUItLOjRiaILz NVzWSGtYWmWmdXP0bY9vKGmwIFHUVEBt\X[nbDDpckBpfW2jbjDTT{1OTUxvMkigZ4VtdHNiYYSgNVAxKHWPIH3hbY51[WmwZXSgbY4hVG:la3Wnd{B{d2y3dHnvckBi\nSncjCyOEBpenNiYomgcJVkcW[ncnHz[U1j[XOnZDDhd5NigSCrbjDhZpNmdmOnIH;mJGdHKGGwZDDncJVkd3On MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh|NUexPUc,OjJ6M{W3NVk9N2F-
A673 M1zrSpFJXFNiYYPzZZk> MXrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDBOlc{KGOnbHzzLS=> NH[ydZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-MC MXfxTHRUKGG|c3H5 MlLOdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgV2suVi2PQzDj[Yxtew>? NFnzUms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
NB-EBc1 MkDmdWhVWyCjc4PhfS=> NHPySm1yUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBPSi2HQnOxJINmdGy| Mke2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
TC32 NEm5WGJyUFSVIHHzd4F6 MXrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDUR|MzKGOnbHzz NVK0cY9CRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
BL21(DE3) MljQSpVv[3Srb36gZZN{[Xl? NYX1b3FvUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBU[2irc4Tvd49u[SCvYX7zc45qKHOrcoT1bY4hOiBqMkGgeI8hOzJ{IILld4llfWW|KTDlfJBz\XO|ZXSgbY4hTXOlaHXybYNpcWFiY3;sbUBDVDJzKFTFN{kh[2WubIOgbY4heHKnc3XuZ4Uhd2ZiWl3BUC=> MnTnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF2OU[yOVEoRjNzNEm2NlUyRC:jPh?=
BL21(DE3) NEHW[JFHfW6ldHnvckBie3OjeR?= NXPzR5piUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBU[2irc4Tvd49u[SCvYX7zc45qKHOrcoT1bY4hOiBqMkGgeI8hOzJ{IILld4llfWW|KTDlfJBz\XO|ZXSgbY4hTXOlaHXybYNpcWFiY3;sbUBDVDJzKFTFN{kh[2WubIOgbY4heHKnc3XuZ4Uhd2ZiKGqpMUhDfXRrTInzMWFOSw>? MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTR7NkK1NUc,OzF2OU[yOVE9N2F-
BL21(DE3) MXTGeY5kfGmxbjDhd5NigQ>? MkfWTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDTZ4hqe3Sxc3;tZUBu[W6|b37pJJNqenS3aX6gNkApOjFidH:gN|IzKHKnc3nkeYV{MSCneIDy[ZN{\WRiaX6gSZNkcGW{aXPobYEh[2:uaTDCUFIyMESHMzmgZ4VtdHNiaX6gdJJme2WwY3Wgc4YhMFprLTjI[ZgqVHm|LVHNRy=> NYTZNIo5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G0PVYzPTFpPkOxOFk3OjVzPD;hQi=>
BL21(DE3) M{jBZWZ2dmO2aX;uJIF{e2G7 NHXh[FhKdmirYnn0bY9vKG:oIILlZ49u[mmwYX70JHNkcGm|dH;zc41iKG2jboPvcokhe2m{dIXpckAzKCh{MTD0c{A{OjJicnXzbYR2\XNrIHX4dJJme3OnZDDpckBGe2OqZYLpZ4hq[SClb3zpJGJNOjFqRFWzLUBk\WyuczDpckBxemW|ZX7j[UBw\iBqWjmtLG9kfCmOeYOtRW1E MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTR7NkK1NUc,OzF2OU[yOVE9N2F-
HEK293T Ml7vSpVv[3Srb36gZZN{[Xl? MkjWOlAhdWmwcx?= MojoTY5pcWKrdHnvckBw\iCVdILldHRi\y22YXfn[YQhcHWvYX6gV2FTVTFiVFnSJEg2PjFidH:gO|I1KHKnc3nkeYV{MSCneIDy[ZN{\WRiaX6gTGVMOjl|VDDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hVkGGYYPlJIFkfGm4aYT5JIlvKHC{ZYPlcoNmKG:oIF7BSEshcW6ldXLheIVlKG[xcjC2NEBucW6|IHL5JGhRVENiYX7hcJl{cXNuIFnDOVA:PDNwON88UU4> MnrFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE1OC9pPlPoSW1DVDxxYU6=
Assay
Methods Test Index PMID
Western blot p-MLC / MLC / p-MYPT1 / MYPT1 ; p-β-catenin / β-catenin ; Sp1 / ERK 30503259 18446063
Immunofluorescence p-MLC 30503259
In vivo In the mouse, nicotinamide given i.p. at doses of 100-500 mg/kg showed biphasic elimination with dose-dependent changes in half-life. The initial half-life increased significantly (P <0.05) from 0.8 to 2 h and the terminal half-life increased from 3.4 to 5.6 h over the dose range studied. Clearance, however, decreased significantly from 0.3 to 0.24 L/kg/h only at the highest dose. Peak concentrations increased in a dose-dependent manner from 1,000 to 4,800 nmol/ml. The bioavailability given via the i.p. as compared with the i. v. route was close to 100%[6].

Protocol (from reference)

Cell Research:

[7]

  • Cell lines: HaCaT cells
  • Concentrations: 33 μM
  • Incubation Time: 7 days and 14 days
  • Method:

    The established cell line of human epidermal keratinocytes (HaCaT cells) was routinely cultured in Dulbecco's modified Eagle medium (DMEM) containing 10% fetal bovine serum and kept in a humidified atmosphere containing 5% CO2 at 37°C. For NAD(P) modulation, cells were grown in DMEM and 10% dialyzed fetal bovine serum and with addition of 33 μM nicotinamide (33 μM Nam) or without added nicotinamide (0 μM Nam). Cell number was measured by counting. Sensitivity to glutaminase inhibition was performed using 0.1 μM 6-diazo-5-oxo-L-norleucine (DON) on cells grown in 33 μM Nam or 0 μM Nam for 7 days.

Animal Research:

[6]

  • Animal Models: Mice (strain CBA/Ht/GyfBSVS)
  • Dosages: 100, 200, 300 and 500 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

DMSO 24 mg/mL
(196.52 mM)
Water 24 mg/mL
(196.52 mM)
Ethanol '24 mg/mL

Chemical Information

Molecular Weight 122.12
Formula

C6H6N2O

CAS No. 98-92-0
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC(=CN=C1)C(=O)N

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04763499 Not yet recruiting Other: freeze dried strawberry powder Vascular Dilation University of California Davis January 1 2022 Not Applicable
NCT05040321 Not yet recruiting Drug: MIB-626|Drug: Placebo Alzheimer''s Disease (Incl Subtypes)|Dementia Brigham and Women''s Hospital December 1 2021 Phase 1|Phase 2
NCT04641663 Enrolling by invitation Dietary Supplement: Multi-target Dietary Supplement (MTDS) Aging|Age-related Cognitive Decline Douglas Boreham|McMaster University|Northern Ontario School of Medicine September 3 2021 Not Applicable
NCT04844528 Suspended Drug: Nicotinamide|Drug: Placebo Chronic Lymphocytic Leukemia University of Utah August 5 2021 Phase 2
NCT04909489 Recruiting Other: cross-sectional study without intervention Traditional Chinese Medicine|Dyslipidemias|Syndrome Dongzhimen Hospital Beijing April 21 2021 --
NCT04342975 Recruiting Drug: Nicotinamide Riboside + Pterostilbene|Drug: Placebo AKI Mayo Clinic|Elysium Health December 1 2020 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

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