Nicotinamide (Vitamin B3)

Catalog No.S1899 Synonyms: Niacinamide, Vitamin PP, Nicotinic acid amide

Nicotinamide (Vitamin B3) Chemical Structure

Molecular Weight(MW): 122.12

Nicotinamide (Vitamin B3), a water-soluble vitamin, is an active component of coenzymes NAD and NADP, and also act as an inhibitor of sirtuins.

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In DMSO USD 130 In stock
USD 97 In stock
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Cited by 2 Publications

1 Customer Review

  • Immunoprecipitation-western blot for analyzing acetylated Snail in Snail-transfected 293T cells (upper) and FaDu-Snail cells (lower) treated with a vehicle control (Ctrl), a HDAC inhibitor TSA 5 nM for 8 hr, a SIRT1 inhibitor NAM(Nicotinamide) 10 mM for 8 hr, or in combination.

    Cancer Cell,2014, 26(4):534-48.. Nicotinamide (Vitamin B3) purchased from Selleck.

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Biological Activity

Description Nicotinamide (Vitamin B3), a water-soluble vitamin, is an active component of coenzymes NAD and NADP, and also act as an inhibitor of sirtuins.
Targets
Sirtuin [1]
In vitro

Nicotinamide strongly inhibits yeast silencing, increases rDNA recombination, and shortens replicative life span to that of a sir2 mutant. Nicotinamide abolishes silencing and leads to an eventual delocalization of Sir2 even in G(1)-arrested cells, demonstrating that silent heterochromatin requires continual Sir2 activity. [1] Nicotinamide results in a twofold increase in DNA content and a threefold increase in insulin content in the fetal cells. Nicotinamide induces differentiation and maturation of human fetal pancreatic islet cells. [2] Nicotinamide regulates sirtuins by switching between deacetylation and base exchange. Nicotinamide switching is quantitated for the Sir2s from Archeaglobus fulgidus (Sir2Af2), Saccharomyces cerevisiae (Sir2p), and mouse (Sir2alpha). [3] Nicotinamide selectively reduces a specific phospho-species of tau (Thr231) that is associated with microtubule depolymerization in Alzheimer's disease transgenic mice, in a manner similar to inhibition of SirT1. Nicotinamide also dramatically increases acetylated alpha-tubulin, a primary substrate of SirT2, and MAP2c in Alzheimer's disease transgenic mice, both of which are linked to increased microtubule stability. [4] Nicotinamide fosters DNA integrity and maintains phosphatidylserine membrane asymmetry to prevent cellular inflammation, cellular phagocytosis and vascular thrombosis. Nicotinamide both prevents and reverses neuronal and vascular cell injury. [5]
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Escherichia coli BL21 (DE3) cells MVrGeY5kfGmxbjDhd5NigQ>? M1;NWGlvcGmkaYTpc44hd2ZiY3H0ZYx6fGmlYXzsfUBi[3SrdnWgbJVu[W5iU1nSWFMhMDFyMjD0c{A{QTliYX3pco8h[WOrZIOpJIV5eHKnc4Pl[EBqdiCHc3Po[ZJq[2irYTDjc4xqKEKOMkGgLGRGOyliY3XscJMhfXOrbneg[ox2d3KxZ3XubYMhPy2jbXnuc{01NW2ndHj5cINwfW2jcnnuJEhCVUNrLXzhZoVt\WRicHXweIll\SCkeTDmcJVwemW|Y3XuZ4Uh[XO|YYmsJGlEPTB;Nj6yJO69VQ>? NGDKdIEzPTJ5NUiyOC=>
human SK-MEL-28 cells NFvpUHNHfW6ldHnvckBie3OjeR?= NGWwUGQyODBizszN M2nYe|I1KGh? Mn76VoVlfWO2aX;uJIlvKEGWUDDs[ZZmdCCrbjDoeY1idiCVSz3NSWwuOjhiY3XscJMh[XRiMUCwJJVOKG2jaX70ZYlv\WRiaX6gUI9kc2VpczDzc4x2fGmxbjDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG|ZT3iZZNm\CCjc4PhfUBqdiCjYoPlcoNmKG:oIFfGJIFv\CCpbIXjc5Nm NIXVdnMzOjh|NUexPS=>

... Click to View More Cell Line Experimental Data
In vivo In the mouse, nicotinamide given i.p. at doses of 100-500 mg/kg showed biphasic elimination with dose-dependent changes in half-life. The initial half-life increased significantly (P <0.05) from 0.8 to 2 h and the terminal half-life increased from 3.4 to 5.6 h over the dose range studied. Clearance, however, decreased significantly from 0.3 to 0.24 L/kg/h only at the highest dose. Peak concentrations increased in a dose-dependent manner from 1,000 to 4,800 nmol/ml. The bioavailability given via the i.p. as compared with the i. v. route was close to 100%[6].

Protocol

Cell Research:

[7]
+ Expand
  • Cell lines: HaCaT cells
  • Concentrations: 33 μM
  • Incubation Time: 7 days and 14 days
  • Method:

    The established cell line of human epidermal keratinocytes (HaCaT cells) was routinely cultured in Dulbecco's modified Eagle medium (DMEM) containing 10% fetal bovine serum and kept in a humidified atmosphere containing 5% CO2 at 37°C. For NAD(P) modulation, cells were grown in DMEM and 10% dialyzed fetal bovine serum and with addition of 33 μM nicotinamide (33 μM Nam) or without added nicotinamide (0 μM Nam). Cell number was measured by counting. Sensitivity to glutaminase inhibition was performed using 0.1 μM 6-diazo-5-oxo-L-norleucine (DON) on cells grown in 33 μM Nam or 0 μM Nam for 7 days.


    (Only for Reference)
Animal Research:

[6]
+ Expand
  • Animal Models: Mice (strain CBA/Ht/GyfBSVS)
  • Formulation: Saline
  • Dosages: 100, 200, 300 and 500 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 24 mg/mL (196.52 mM)
Water 24 mg/mL (196.52 mM)
Ethanol 24 mg/mL (196.52 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 122.12
Formula

C6H6N2O
CAS No. 98-92-0
Storage powder
in solvent
Synonyms Niacinamide, Vitamin PP, Nicotinic acid amide

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03432871 Recruiting Mitochondrial Diseases|Mitochondrial Myopathies|Progressive External Ophthalmoplegia|Progressive Ophthalmoplegia|Progressive; Ophthalmoplegia External|Mitochondria DNA Deletion|MELAS|Mitochondrial Encephalomyopathy Lactic Acidosis and Stroke-Like Episodes|Mitochondrial Encephalopathy Lactic Acidosis and Stroke-Like Episodes (MELAS Syndrome) Cambridge University Hospitals NHS Foundation Trust|University of Cambridge|MRC Mitochondrial Biology Unit December 8 2017 Not Applicable
NCT02303483 Completed Obese Aarhus University Hospital|University of Copenhagen January 4 2016 Not Applicable
NCT03260166 Recruiting Cutaneous Lupus Erythematosus|Systemic Lupus Erythematosus Rash Second Xiangya Hospital of Central South University|National Natural Science Foundation of China|Hunan Provincial Natural Science Foundation of China|National Key Clinical Specialty Construction Project of China August 31 2017 Phase 2
NCT02942888 Recruiting Mild Cognitive Impairment|NAD The University of Texas Health Science Center at San Antonio|University of Texas|South Texas Veterans Health Care System November 30 2017 Not Applicable
NCT03565328 Recruiting Heart Failure National Heart Lung and Blood Institute (NHLBI)|National Institutes of Health Clinical Center (CC) September 27 2018 Phase 2
NCT02812238 Completed Atherosclerosis|Diabetes|Coronary Artery Disease National Heart Lung and Blood Institute (NHLBI)|National Institutes of Health Clinical Center (CC) June 23 2016 Phase 2

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Sirtuin Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID