Nicotinamide (Vitamin B3)

Name: Nicotinamide (Vitamin B3)
Brand: Selleck Chemicals
SKU: S1899
Rating: 5 (15 reviews)

For research use only.

Catalog No.S1899 Synonyms: Niacinamide, Vitamin PP, Nicotinic acid amide

15 publications

Nicotinamide (Vitamin B3) Chemical Structure

CAS No. 98-92-0

Nicotinamide (Vitamin B3, Niacinamide, Vitamin PP, Nicotinic acid amide), a water-soluble vitamin, is an active component of coenzymes NAD and NADP, and also act as an inhibitor of sirtuins.

Selleck's Nicotinamide (Vitamin B3) has been cited by 15 publications

Cancer Cell, 2014, 26(4):534-48

PubMed: 25314079 ( click the link to review the publication )

PLoS Pathog, 2020, 16;16(3):e1008341

PubMed: 32176725 ( click the link to review the publication )

Theranostics, 2020, 10(17):7747-7757

PubMed: 32685017 ( click the link to review the publication )

J Exp Clin Cancer Res, 2020, 39(1):142

PubMed: 32711549 ( click the link to review the publication )

Mol Cell, 2019, 73(4):788-802

PubMed: 30704899 ( click the link to review the publication )

Nat Commun, 2019, 10(1):5792

PubMed: 31857589 ( click the link to review the publication )

Nucleic Acids Res, 2019, 19;47(16):8502-8520

PubMed: 31616951 ( click the link to review the publication )

Cell Death Dis, 2019, 10(5):363

PubMed: 31043584 ( click the link to review the publication )

Pharmacol Res, 2019, 146:104281

PubMed: 31125601 ( click the link to review the publication )

World J Gastroenterol, 2019, 25(38):5800-5813

PubMed: 31636473 ( click the link to review the publication )

Eur J Pharmacol, 2019, 854:328-337

PubMed: 31028741 ( click the link to review the publication )

Sci Adv, 2019, 5(3):eaav1118

PubMed: 30944854 ( click the link to review the publication )

Cancer Immunol Res, 2018, 6(10):1220-1233

PubMed: 30131377 ( click the link to review the publication )

Redox Biol, 2018, 15:62-73

PubMed: 29220696 ( click the link to review the publication )

PLoS Biol, 2013, 12(1):e1001758

PubMed: 24409098 ( click the link to review the publication )

1 Customer Review

Immunoprecipitation-western blot for analyzing acetylated Snail in Snail-transfected 293T cells (upper) and FaDu-Snail cells (lower) treated with a vehicle control (Ctrl), a HDAC inhibitor TSA 5 nM for 8 hr, a SIRT1 inhibitor NAM(Nicotinamide) 10 mM for 8 hr, or in combination.

Cancer Cell,2014, 26(4):534-48.. Nicotinamide (Vitamin B3) purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description

Nicotinamide (Vitamin B3, Niacinamide, Vitamin PP, Nicotinic acid amide), a water-soluble vitamin, is an active component of coenzymes NAD and NADP, and also act as an inhibitor of sirtuins.

Targets

Sirtuin ^[1]

In vitro

Nicotinamide strongly inhibits yeast silencing, increases rDNA recombination, and shortens replicative life span to that of a sir2 mutant. Nicotinamide abolishes silencing and leads to an eventual delocalization of Sir2 even in G(1)-arrested cells, demonstrating that silent heterochromatin requires continual Sir2 activity. ^[1] Nicotinamide results in a twofold increase in DNA content and a threefold increase in insulin content in the fetal cells. Nicotinamide induces differentiation and maturation of human fetal pancreatic islet cells. ^[2] Nicotinamide regulates sirtuins by switching between deacetylation and base exchange. Nicotinamide switching is quantitated for the Sir2s from Archeaglobus fulgidus (Sir2Af2), Saccharomyces cerevisiae (Sir2p), and mouse (Sir2alpha). ^[3] Nicotinamide selectively reduces a specific phospho-species of tau (Thr231) that is associated with microtubule depolymerization in Alzheimer's disease transgenic mice, in a manner similar to inhibition of SirT1. Nicotinamide also dramatically increases acetylated alpha-tubulin, a primary substrate of SirT2, and MAP2c in Alzheimer's disease transgenic mice, both of which are linked to increased microtubule stability. ^[4] Nicotinamide fosters DNA integrity and maintains phosphatidylserine membrane asymmetry to prevent cellular inflammation, cellular phagocytosis and vascular thrombosis. Nicotinamide both prevents and reverses neuronal and vascular cell injury. ^[5]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
Escherichia coli BL21 (DE3) cells	Mkf2SpVv[3Srb36gZZN{[Xl?				M{n5TWlvcGmkaYTpc44hd2ZiY3H0ZYx6fGmlYXzsfUBi[3SrdnWgbJVu[W5iU1nSWFMhMDFyMjD0c{A{QTliYX3pco8h[WOrZIOpJIV5eHKnc4Pl[EBqdiCHc3Po[ZJq[2irYTDjc4xqKEKOMkGgLGRGOyliY3XscJMhfXOrbneg[ox2d3KxZ3XubYMhPy2jbXnuc{01NW2ndHj5cINwfW2jcnnuJEhCVUNrLXzhZoVt\WRicHXweIll\SCkeTDmcJVwemW\|Y3XuZ4Uh[XO\|YYmsJGlEPTB;Nj6yJO69VQ>?	MlLINlUzPzV6MkS=
human SK-MEL-28 cells	NGLweVJHfW6ldHnvckBie3OjeR?=	NG\ROGEyODBizszN	M1HnWVI1KGh?		M1\FN3Jm\HWldHnvckBqdiCDVGCgcIV3\WxiaX6gbJVu[W5iU1utUWVNNTJ6IHPlcIx{KGG2IEGwNEB2VSCvYXnueIFqdmWmIHnuJGxw[2unJ4Ogd49tfXSrb36gZYZ1\XJiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WtZoF{\WRiYYPzZZkhcW5iYXLz[Y5k\SCxZjDHSkBidmRiZ3z1Z49{\Q>?	M2m0[\|IzQDN3N{G5

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	p-MLC / MLC / p-MYPT1 / MYPT1 ; PubMed: 30503259 Stem Cell Reports Dose-dependent effect of nicotinamide on the phosphorylation of MYPT1 (Thr 696) and MLC (Ser 19). Individualized hESCs were treated with nicotinamide at indicated concentrations for 1 hr. Top, western blot image. Bottom, quantification of the western blot results (n = 3). p-β-catenin / β-catenin ; PubMed: 30503259 Stem Cell Reports The phosphorylation of β-catenin was decreased by 10 mM nicotinamide or 5 μM CK1 inhibitor D4476 treatment for 6 hr (n = 3) as shown by western blot. Sp1 / ERK ; PubMed: 18446063 Exp Mol Med Cells were either mock-treated or treated with nicotinamide at the concentration either of 10 or 20 mM, collected at the indicated time points, and applied to Western blotting analysis.	30503259 18446063
Immunofluorescence	p-MLC; PubMed: 30503259 Stem Cell Reports Confocal images of individualized hESCs treated with 10 mM nicotinamide (Nam) or 10 μM ROCK inhibitor Y27632 (ROCKi). Red, phalloidin 594; green, p-MLC (Ser19). Scale bar, 10 μm.	30503259

In vivo

In the mouse, nicotinamide given i.p. at doses of 100-500 mg/kg showed biphasic elimination with dose-dependent changes in half-life. The initial half-life increased significantly (P <0.05) from 0.8 to 2 h and the terminal half-life increased from 3.4 to 5.6 h over the dose range studied. Clearance, however, decreased significantly from 0.3 to 0.24 L/kg/h only at the highest dose. Peak concentrations increased in a dose-dependent manner from 1,000 to 4,800 nmol/ml. The bioavailability given via the i.p. as compared with the i. v. route was close to 100%^[6].

Protocol

Cell Research:

^[7]

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Cell lines: HaCaT cells
Concentrations: 33 μM
Incubation Time: 7 days and 14 days
Method:
The established cell line of human epidermal keratinocytes (HaCaT cells) was routinely cultured in Dulbecco's modified Eagle medium (DMEM) containing 10% fetal bovine serum and kept in a humidified atmosphere containing 5% CO2 at 37°C. For NAD(P) modulation, cells were grown in DMEM and 10% dialyzed fetal bovine serum and with addition of 33 μM nicotinamide (33 μM Nam) or without added nicotinamide (0 μM Nam). Cell number was measured by counting. Sensitivity to glutaminase inhibition was performed using 0.1 μM 6-diazo-5-oxo-L-norleucine (DON) on cells grown in 33 μM Nam or 0 μM Nam for 7 days.

(Only for Reference)

Animal Research:

[6]

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Animal Models: Mice (strain CBA/Ht/GyfBSVS)
Dosages: 100, 200, 300 and 500 mg/kg
Administration: i.p.
(Only for Reference)

References

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Solubility (25°C)

In vitro	DMSO	24 mg/mL (196.52 mM)
	Water	24 mg/mL (196.52 mM)
	Ethanol	'24 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Nicotinamide (Vitamin B3) SDF

Molecular Weight	122.12
Formula	C₆H₆N₂O
CAS No.	98-92-0
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	Niacinamide, Vitamin PP, Nicotinic acid amide
Smiles	C1=CC(=CN=C1)C(=O)N

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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The Serial Dilution Calculator Equation

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Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
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Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2020-09-01)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04342975	Recruiting	Drug: Nicotinamide Riboside + Pterostilbene\|Drug: Placebo	AKI	Mayo Clinic\|Elysium Health	September 2020	Phase 2
NCT04387201	Recruiting	Drug: Dulaglutide\|Drug: Cyanocobalamin	Glucose Intolerance\|Overweight and Obesity\|Drug Effect\|Adiposity	The University of Texas Health Science Center Houston	May 15 2020	Phase 4
NCT04110028	Recruiting	Drug: Nicotinamide riboside\|Drug: Placebo	Inflammation\|Acute Illness	Oslo University Hospital\|ChromaDex Inc.	October 1 2019	Phase 1\|Phase 2
NCT03975777	Recruiting	Other: LO\|Other: HI	Mitochondrial Biogenesis	Brendon Gurd PhD\|Queen''s University	June 1 2019	Not Applicable
NCT03870035	Not yet recruiting	--	PCOS	Assiut University	May 1 2019	--
NCT03838822	Completed	Dietary Supplement: Cofactors	Healthy	Sahlgrenska University Hospital Sweden\|Karolinska Institutet\|Chalmers University of Technology	September 1 2018	Early Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Nicotinamide (Vitamin B3)