For research use only.

Catalog No.S7751 Synonyms: C07

31 publications

VER155008 Chemical Structure

CAS No. 1134156-31-2

VER155008 (C07) is a potent Hsp70 family inhibitor with IC50 of 0.5 μM, 2.6 μM, and 2.6 μM in cell-free assays for HSP70, HSC70, and GRP78 (HSPA5, Bip), respectively, >100-fold selectivity over HSP90. VER155008 inhibits autophagy and causes reduced levels of HSP90 client proteins.

Selleck's VER155008 has been cited by 31 publications

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Biological Activity

Description VER155008 (C07) is a potent Hsp70 family inhibitor with IC50 of 0.5 μM, 2.6 μM, and 2.6 μM in cell-free assays for HSP70, HSC70, and GRP78 (HSPA5, Bip), respectively, >100-fold selectivity over HSP90. VER155008 inhibits autophagy and causes reduced levels of HSP90 client proteins.
HSP70 [1]
(Cell-free assay)
HSC70 [1]
(Cell-free assay)
GRP78 [1]
(Cell-free assay)
0.5 μM 2.6 μM 2.6 μM
In vitro

VER-155008 inhibits the proliferation of human breast and colon cancer cell lines BT474, MB-468, HCT116, and HT29 with GI50s in the range 5.3-14.4 μM, and induces Hsp90 client protein degradation in both HCT116 and BT474 cells.[1] In the 8505C and FRO cells, VER-155008 reduces the cell viability and elevates the percentage of dead cells in a time- and dose- dependent manner.[2] VER-155008 causes a dose-dependent inhibition of cytokine-dependent AML cell proliferation.[3] VER-155008 shows an effective inhibition of cell proliferation in A549 and H1975 cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
BL21 DE3 MofDSpVv[3Srb36gZZN{[Xl? MV2xNEBucW6| NWPZSW9MUW6qaXLpeIlwdiCxZjDHVnA4QCBqMk[geI8hPjN4IILld4llfWW|KTCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBGe2OqZYLpZ4hq[SClb3zpJGJNOjFiRFWzJINmdGy|IIDy[U1qdmO3YnH0[YQh\m:{IEGwJI1qdnNiYnXmc5JmKE[LVFOtUnJNVEyWRzDmcJVwemW|Y3XueEBx\XC2aXTlJIFl\Gm2aX;uJIlvKHC{ZYPlcoNmKG:oIEGwJJVOKEGGUDDmc4xtd3enZDDifUBnfXK2aHXyJIlv[3WkYYTpc44h\m:{IEKgbJJ{KGK7IH\seY9z\XOlLDDJR|UxRTBwON88US=> NVPNeXRMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{GxNlkxPTRpPkOxNVI6ODV2PD;hQi=>
HCT116 MVTDfZRwfG:6aXPpeJkh[XO|YYm= NXfRU2ExS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWMUG2JINmdGy|IHL5JHNTSiCjc4PhfUwhT0l3ME21{txO MoHrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl{NU[1NFgoRjF7MkW2OVA5RC:jPh?=
HCT116 MXrBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MYnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiFVEGxOkBk\WyuczygS2k2OD13zszN MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTV{Nke2N{c,OjF3Mk[3OlM9N2F-
BT474 MlrTSpVv[3Srb36gZZN{[Xl? M1Xoc2JqdmSrbnegZYZncW6rdImgeI8hUHOyN{CgcpVkdGWxdHnk[UBjcW6maX7nJIRwdWGrbjDpckBpfW2jbjDCWFQ4PCClZXzsd{whUUN3ME2xNE41|ryP NEPPNGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{ME[wPFc{QCd-MkC2NFg4Ozh:L3G+
HCT116 NUS0[XNDTnWwY4Tpc44h[XO|YYm= M4rRXVI1KGi{cx?= NILRXYdKdmirYnn0bY9vKG:oIFjTVFcxKGmwIHj1cYFvKEiFVEGxOkBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hd2ZiSHWyJJBzd3SnaX6gcIV3\Wy|IHHmeIVzKDJ2IHjyd{BjgSCZZYP0[ZJvKGKub4S= MoTuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl{NU[1NFgoRjF7MkW2OVA5RC:jPh?=
HCT116 MWnGeY5kfGmxbjDhd5NigQ>? MkG3NlQhcHK| MYfJcohq[mm2aX;uJI9nKEiVUEewJIlvKGi3bXHuJGhEXDFzNjDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gc4YhWmGoMTDwdo91\WmwIHzleoVteyCjZoTldkAzPCCqcoOgZpkhX2W|dHXyckBjdG:2 NH:zXm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUK1OlUxQCd-MUmyOVY2ODh:L3G+

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
Hsp70 / AR; 

PubMed: 28691182     

LNCaP95 cells were treated with VER155008 (25 μM) for the indicated times. Protein extracts (15 μg) were subjected to Western blotting.


PubMed: 28842558     

SUDHL-2 cells or RJ-Lym1 cells were treated with MG132 (1 μM) or VER155008 (30 μM) for 24 h, stained with a Blimp-1 antibody (red) and DAPI (blue), and then observed under a confocal microscope. Scale bar: 5 μm.

Growth inhibition assay
Cell viability; 

PubMed: 28053100     

Vero cells were treated with the indicated concentrations of VER155008 for 96 h, and then cell viability was determined and calculated as a percentage of the viability of cells treated with DMSO. 

In vivo In HCT116 tumor bearing mice, VER-155008 (25 or 40 mg/kg, i.v.) demonstrates rapid metabolism and clearance, along with tumor levels below the predicted pharmacologically active level.[1]


Kinase Assay:[1] [5]
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Hsc70, Hsp70 and Grp78 fluorescence polarisation (FP) assay:

The FP assay for Hsp70 is conducted in aqueous buffer consisting of 100 mM Tris pH 7.4, 150 mM KCl and 5 mM CaCl2, in a final assay volume of 100 µl, using 96 well black polystyrene high bind plates with a Fusion plate reader. N6-(6-amino)hexyl-ATP-5-FAM and the in-house protein preparation of GST-HSP70 3-382 have final concentrations in the assay of 20 nM and 400 nM, respectively. Compounds are tested as 10-point IC50s, with a final DMSO concentration of 5%. Assay mixtures are incubated for 3 h prior to reading on the Fusion (ex 485 nm; em 535 nm). The data is fitted using a 4 parameter logistical data model by XLFit 4. The FP assay for Hsc70 and Grp78 is carried out as described for Hsp70 using the same N6 -(6-amino)hexyl-ATP-5-FAM as the FP probe with the following modifications. For Hsc70, the protein and probe concentrations are 0.3 μM and 20 nM, respectively with a 30 min incubation at 22℃ while for Grp78, the protein and probe concentrations are 2 μM and 10 nM, respectively with a 2 h incubation at 22℃. The KD for the FAM-ATP probe was 0.24 μM for Hsc70 and 2 μM for Grp78.
Cell Research:[1]
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  • Cell lines: BT474, MB-468, HCT116, and HT29 cells
  • Concentrations: 50 μM
  • Incubation Time: 72 h
  • Method: All cell lines are grown in DMEM/10% FCS with GlutaMAX-I in a humidified atmosphere of 5% CO2 in air. Cell proliferation is determined using the sulforhodamine B (SRB) assay.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: HCT116 tumor bearing
  • Dosages: 40 mg/kg
  • Administration: i.v.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (179.72 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 556.4


CAS No. 1134156-31-2
Storage powder
in solvent
Synonyms C07
Smiles C1=CC(=CC=C1COCC2C(C(C(O2)N3C4=NC=NC(=C4N=C3NCC5=CC(=C(C=C5)Cl)Cl)N)O)O)C#N

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Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID