Home Metabolism HSP (HSP90) modulator TRC051384 For research use only.

TRC051384

TRC051384 is an inducer of heat shock protein 70 (HSP70) and potent anti-inflammatory agent.

TRC051384 Chemical Structure

TRC051384 Chemical Structure

CAS: 867164-40-7

Selleck's TRC051384 has been cited by 3 publications

Purity & Quality Control

Batch: S830501 DMSO] 93 mg/mL] false] Ethanol] 5 mg/mL] false] Water] Insoluble] false Purity: 99.06%
99.06

TRC051384 Related Products

Signaling Pathway

HSP (HSP90) Signaling Pathways

HSP (HSP90) Signaling Pathway

Choose Selective HSP (HSP90) Inhibitors

Biological Activity

Description TRC051384 is an inducer of heat shock protein 70 (HSP70) and potent anti-inflammatory agent.
Targets
HSP70 [1]
In vitro
In vitro TRC051384 is a compound that induces HSPA1A/B expression and subsequent Hsp70 production via heat shock factor-1 (HSF1) activation[2]. TRC051384 increases HSP70B mRNA expression in both HeLa and primary mixed neuronal cells. Induction of HSP70 by TRC051384 involves HSF1 activation and results in elevated chaperone and anti-inflammatory activity[1].
Cell Research Cell lines HeLa cell line (CCL-2, ATCC)
Concentrations 6.25 and 12.5 μM
Incubation Time 4 h
Method

Induction of HSP70B mRNA is carried out by treatment of cells with TRC051384 (6.25 and 12.5 μM) for 4 hours duration for HeLa cell line and total RNA is isolated. For all RNA samples, cDNAs are synthesized and expression of HSP70B mRNA along with expression of 18S rRNA is monitored by real-time PCR employing ABI 7000 system.
In Vivo
In vivo Treatment with TRC051384 significantly reduces stroke associated neuronal injury and disability in a rat model of transient ischemic stroke even when administered 8 hours post onset of ischemia. TRC051384 significantly reduces infarct size, brain edema and improves neurological deficit[1]. TRC051384 delays thrombus formation in WT mice, the tail bleeding time still being unaltered[2].
Animal Research Animal Models Sprague Dawley male rats
Dosages 4.5 mg/kg and 9 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 465.54 Formula

C25H31N5O4
CAS No. 867164-40-7 SDF Download TRC051384 SDF
Smiles C1COCCN1CCNC(=O)NC2=CC=C(C=C2)C(=O)C=CC3=NC(=CC=C3)N4CCOCC4
Storage (From the date of receipt) 3 years -20°C(in the dark) powder

In vitro
Batch:

DMSO : 93 mg/mL ( (199.76 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 5 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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