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JG98 HSP inhibitor

Name: JG98
Brand: Selleck Chemicals
SKU: S6721
Availability: InStock
Rating: 5 (4 reviews)

Cat.No.S6721

JG-98 is an allosteric inhibitor of Hsp70 that binds tightly to a deep pocket that is conserved in members of the Hsp70 family. JG-98 induces classical apoptosis features, including morphological changes consistent with programmed cell death and positive annexin staining. JG-98 exhibits anticancer activity.

Chemical Structure

Molecular Weight: 534.54

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Quality Control

Batch: Purity: 99.91%

99.91

Related Targets	Akt Wnt/beta-catenin PKC ROCK Microtubule Associated Integrin Bcr-Abl Actin FAK Kinesin
Other HSP Inhibitors	Tanespimycin (17-AAG) Elesclomol (STA-4783) Luminespib (NVP-AUY922) Ganetespib (STA-9090) Alvespimycin (17-DMAG) Hydrochloride VER-155008 Onalespib (AT13387) BIIB021 Zelavespib (PU-H71) HSP990 (NVP-HSP990)

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
MDA-MB-231	Antiproliferative assay			Antiproliferative activity against human MDA-MB-231 cells by MTT assay, IC50=0.39μM.	29953808
MDA-MB-231	Cytotoxicity assay		72 hrs	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay, EC50=0.4μM.	24312699
MCF7	Cytotoxicity assay		72 hrs	Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTT assay, EC50=0.7μM.	24312699
MCF7	Antiproliferative assay			Antiproliferative activity against human MCF7 cells by MTT assay, IC50=0.71μM.	29953808
MEF	Antiproliferative assay			Antiproliferative activity against MEF cells by MTT assay, IC50=4.2μM.	29953808
MDA-MB-231	Autophagic assay	5 to 10 uM	24 hrs	Induction of autophagic flux in human MDA-MB-231 cells assessed as increase in p62 oligomerization at 5 to 10 uM after 24 hrs	24312699
MDA-MB-231	Apoptosis assay	10 uM	48 hrs	Induction of apoptosis in human MDA-MB-231 cells assessed as caspase-3 cleavage at 10 uM after 48 hrs	24312699
MCF7	Autophagic assay	5 to 10 uM	24 hrs	Induction of autophagic flux in human MCF7 cells assessed as increase in p62 oligomerization at 5 to 10 uM after 24 hrs	24312699
MDA-MB-231	Apoptosis assay	10 uM	48 hrs	Induction of apoptosis in human MDA-MB-231 cells assessed as PARP cleavage at 10 uM after 48 hrs	24312699
MCF7	Autophagic assay	5 to 10 uM	24 hrs	Induction of autophagic flux in human MCF7 cells assessed as decrease in p62 monomer level at 5 to 10 uM after 24 hrs	24312699
MDA-MB-231	Autophagic assay	5 to 10 uM	24 hrs	Induction of autophagic flux in human MDA-MB-231 cells assessed as decrease in p62 monomer level at 5 to 10 uM after 24 hrs	24312699
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 10 mg/mL (18.7 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.250mg/ml (0.47mM)	Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.125mg/ml (0.23mM)	Taking the 1 mL working solution as an example, add 50 μL of 2.5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	534.54	Formula	C₂₄H₂₁Cl₂N₃OS₃	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	1456551-16-8	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	CCN1C(=CC2=[N+](C=CS2)CC3=CC=CC=C3)SC(=C4N(C5=C(S4)C=C(C=C5)Cl)C)C1=O.[Cl-]

Mechanism of Action

Targets/IC₅₀/Ki	Hsp70
References	[1] https://www.nature.com/articles/s41598-017-14900-0

Tech Support

Handling Instructions

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