Alvespimycin (17-DMAG) HCl

Name: Alvespimycin (17-DMAG) HCl
Brand: Selleck Chemicals
SKU: S1142
Rating: 5 (48 reviews)

For research use only.

Catalog No.S1142 Synonyms: NSC 707545,BMS 826476 HCl,KOS 1022

48 publications

Alvespimycin (17-DMAG) HCl Chemical Structure

CAS No. 467214-21-7

Alvespimycin (17-DMAG, NSC 707545, BMS 826476, KOS 1022) HCl is a potent HSP90 inhibitor with IC50 of 62 nM in a cell-free assay. Phase 2.

Selleck's Alvespimycin (17-DMAG) HCl has been cited by 48 publications

Transl Oncol, 2021, 14(1):100982

PubMed: 33395748 ( click the link to review the publication )

Nat Commun, 2020, 22;11(1):1935

PubMed: 32321921 ( click the link to review the publication )

Aging Cell, 2020, e13269

PubMed: 33145977 ( click the link to review the publication )

Cancers (Basel), 2020, 12(11)E3335

PubMed: 33187254 ( click the link to review the publication )

Cancers (Basel), 2020, 12(9)E2630

PubMed: 32942617 ( click the link to review the publication )

J Mol Cell Biol, 2020, 24;12(3):216-229

PubMed: 31408169 ( click the link to review the publication )

Hum Mutat, 2020, 10.1002/humu.24147

PubMed: 33252173 ( click the link to review the publication )

Front Oncol, 2020, 10:117

PubMed: 32117764 ( click the link to review the publication )

Life Sci, 2020, 15;249:117532

PubMed: 32151689 ( click the link to review the publication )

J Bioenerg Biomembr, 2020, 15

PubMed: 32291605 ( click the link to review the publication )

Theranostics, 2020, 10(18):8415-8429

PubMed: 32724478 ( click the link to review the publication )

Yonsei Med J, 2020, 61(7):587-596

PubMed: 32608202 ( click the link to review the publication )

Nat Commun, 2019, 10(1):3562

PubMed: 31395886 ( click the link to review the publication )

Mol Cancer Ther, 2019, 18(11):2097-2110

PubMed: 31395684 ( click the link to review the publication )

Mol Pharm, 2019, 16(5):2069-2082

PubMed: 30916978 ( click the link to review the publication )

Nan Fang Yi Ke Da Xue Xue Bao, 2019, 39(2):169-174

PubMed: 30890504 ( click the link to review the publication )

Hepatology, 2019, 69(2):573-586

PubMed: 29356025 ( click the link to review the publication )

Nat Commun, 2018, 9(1):4296

PubMed: 30327466 ( click the link to review the publication )

Nat Commun, 2018, 9(1):672

PubMed: 29445180 ( click the link to review the publication )

Mol Syst Biol, 2018, 14(3):e7858

PubMed: 29507054 ( click the link to review the publication )

Biomed Pharmacother, 2018, 105:1026-1032

PubMed: 30021337 ( click the link to review the publication )

Nat Commun, 2017, 8(1):422

PubMed: 28871086 ( click the link to review the publication )

Mol Cancer Ther, 2017,

PubMed: 28619760 ( click the link to review the publication )

Int J Biol Sci, 2017, 13(4):505-517

PubMed: 28529458 ( click the link to review the publication )
Experimental Cell Research, 2017, 353(1):46-53

PubMed: 28279658 ( click the link to review the publication )

Am J Transl Res, 2017, 9(11):4945-4953

PubMed: 29218092 ( click the link to review the publication )

Cell Death Dis, 2016, 7(6):e2290

PubMed: 27362807 ( click the link to review the publication )

Sci Rep, 2016, 6:19004

PubMed: 26743233 ( click the link to review the publication )

Sci Rep, 2016, 6:38072

PubMed: 27909289 ( click the link to review the publication )

J Biol Chem, 2016,

PubMed: 27489107 ( click the link to review the publication )

Cell Stress Chaperones, 2016, 21(2):339-48

PubMed: 26786409 ( click the link to review the publication )

Medicinal Chemistry Research, 2016, 10.1007/s00044-016-1553-7

PubMed: ( click the link to review the publication )

Viruses, 2016, 8(11)

PubMed: 27801778 ( click the link to review the publication )

Oncotarget, 2016, 7(1):433-45

PubMed: 26595521 ( click the link to review the publication )

Molecules, 2015, 20(1):1643-60

PubMed: 25608045 ( click the link to review the publication )

Immunopharmacol Immunotoxicol, 2015, 8:1-10

PubMed: 26642940 ( click the link to review the publication )

Oncotarget, 2015, 6(14):12128-40

PubMed: 25900240 ( click the link to review the publication )

PLoS One, 2014, 9(12):e114000

PubMed: 25486409 ( click the link to review the publication )

Oncotarget, 2014, 5(13):4920-8

PubMed: 24952482 ( click the link to review the publication )

Zhongguo Fei Ai Za Zhi, 2014, 17(11):778-82

PubMed: 25404267 ( click the link to review the publication )

Assay Drug Dev Technol, 2014, 12(9-10):514-26

PubMed: 25506801 ( click the link to review the publication )

Hepatology, 2013, 57(1):70-80

PubMed: 22898980 ( click the link to review the publication )

Mol Oncol, 2013, 7(6):1093-102

PubMed: 23993685 ( click the link to review the publication )

FEBS J, 2013, 10.1111/febs.12672

PubMed: 24304935 ( click the link to review the publication )

PLoS One, 2013, 8(4):e59315

PubMed: 23565147 ( click the link to review the publication )

Tumour Biol, 2013, 35(4):3229-35

PubMed: 24293393 ( click the link to review the publication )

J Thorac Oncol, 2012, 7(7):1078-85

PubMed: 22592212 ( click the link to review the publication )

Eukaryot Cell, 2012, 11(11):1324-32

PubMed: 22822234 ( click the link to review the publication )

Purity & Quality Control

Choose Selective HSP (HSP90) Inhibitors

Biological Activity

Description

Alvespimycin (17-DMAG, NSC 707545, BMS 826476, KOS 1022) HCl is a potent HSP90 inhibitor with IC50 of 62 nM in a cell-free assay. Phase 2.

Features

A synthetic derivative Geldanamycin, with lower hepatotoxicity than parent antibiotic & higher potency and bioavailability than the similar derivative 17-AAG.

Targets

HSP90 ^[1]
(Cell-free assay)

62 nM

In vitro

17-DMAG displays ~2 times potency against human Hsp90 than 17-AAG, with IC50 of 62 nM versus 119 nM. In SKBR3 and SKOV3 cells which over-express Hsp90 client protein Her2, 17-DMAG causes down-regulation of Her2 with EC50 of 8 nM and 46 nM, respectively, as well as induction of Hsp70 with EC50 of 4 nM and 14 nM, respectively, leading to significant cytotoxicity with GI50 of 29 nM and 32 nM, respectively, consistent with Hsp90 inhibition. ^[1] 17-DMAG in combination with vorinostat synergistically induces apoptosis of the cultured MCL cells as well as primary MCL cells, more potently than either agent alone, by markedly attenuating the levels of cyclin D1 and CDK4, as well as of c-Myc, c-RAF and Akt. ^[3] In contrast to 17-AAG which is only active for IKKβ in chronic lymphocytic leukemia (CLL) cells, 17-DMAG treatment effectively leads to depletion of the Hsp90 client protein, resulting in diminished NF-κB p50/p65 DNA binding, decreased NF-κB target gene transcription, and caspase-dependent apoptosis. By targeting the NF-κB family, 17-DMAG selectively mediates dose- and time-dependent cytotoxicity against CLL cells, but not normal T cells or NK cells important for immune surveillance. ^[5]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
SKBr3	NEDrSZVEgXSxdH;4bYNqfHliYYPzZZk>			NX7JXGVlS3m2b4TvfIlkcXS7IHHnZYlve3RiU1vCdlMh[2WubIOsJGlEPTB;MD6wNlTPxE1w	MmrZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZzNkWzOVQoRjF4MU[1N\|U1RC:jPh?=
SKBR3	Mnu4SpVv[3Srb36gZZN{[Xl?			M3LVW3VxemWpdXzheIlwdiCxZjDId5A4OCCrbjDTT2JTOyClZXzsd{whTUN3ME2wMlAxPM7:TT6=	MnvRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ6NUSwOlYoRjF4OEW0NFY3RC:jPh?=
SKBR3	Mo[wSpVv[3Srb36gZZN{[Xl?			MoPtSIVoemGmYYTpc44hd2ZiSHXyNkBqdiCVS1LSN{Bk\WyuczygSWM2OD1yLkCwPO69VS5?	M1H2RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OEW0NFY3Lz5zNki1OFA3PjxxYU6=
SKOV3	M2qzdmZ2dmO2aX;uJIF{e2G7			Mn7NWZBz\We3bHH0bY9vKG:oIFjzdFcxKGmwIGPLU3Y{KGOnbHzzMEBGSzVyPUCuNFE1\|ryPLh?=	M1;BbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OEW0NFY3Lz5zNki1OFA3PjxxYU6=
SKOV3	MUnGeY5kfGmxbjDhd5NigQ>?			MXrE[Ydz[WSjdHnvckBw\iCKZYKyJIlvKFONT2[zJINmdGy\|LDDFR\|UxRTBwMES2{txONg>?	NUnpTpZ7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[4OVQxPjZpPkG2PFU1ODZ4PD;hQi=>
AGS	NVLnN\|JqTnWwY4Tpc44h[XO\|YYm=		NY\DW4hPOTZiaILz	MkjwTY5pcWKrdHnvckBw\iCKSV[xJIFkfGm4YYTpc44hcW5iaIXtZY4hSUeVIHPlcIx{KGG\|c3Xzd4VlKGG\|IHnubIljcXSrb36gc4YhcHmyb4jpZU1qdmS3Y3XkJIx2[2moZYLhd4Uh\XiycnXzd4lwdiCjZoTldkAyPiCqcoOgZpkhemWyb4L0[ZIh[XO\|YYmsJGlEPTB;MD6wN\|bPxE1w	NYnWTopDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PFM6PTBpPkG3OVg{QTVyPD;hQi=>
Hep3B	NYLufVF7TnWwY4Tpc44h[XO\|YYm=		NE\Sb4cyPiCqcoO=	NWPLfmFXUW6qaXLpeIlwdiCxZjDITWYyKGGldHn2ZZRqd25iaX6gbJVu[W5iSHXwN2Ih[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDofZBwgGmjLXnu[JVk\WRibIXjbYZmemG\|ZTDlfJBz\XO\|aX;uJIFnfGW{IEG2JIhzeyCkeTDy[ZBwenSncjDhd5NigSxiSVO1NF0xNjB4Md88UU4>	MnrhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3OEO5OVAoRjF5NUizPVUxRC:jPh?=
AGS	NUW4VGhsTnWwY4Tpc44h[XO\|YYm=		MmnJNlQhcHK\|	NUTjfWU1XmmjYnnsbZR6KG:oIHj1cYFvKEGJUzDj[YxteyC3bnTldkBvd3Kvb4jpZ{Bkd26maYTpc45{KGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVbPxE1w	MmDSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3OEO5OVAoRjF5NUizPVUxRC:jPh?=
NCI-H526	MkLmSpVv[3Srb36gZZN{[Xl?	NITieWoyKHWP	MUSyOEBpenN?	NY\1TFNtSmmwZHnu[{Bi\m[rbnn0fUB1dyCKU2C5NEBqdiCqdX3hckBPS0lvSEWyOkBk\WyuczDheEAyKHWPIHHmeIVzKDJ2IHjyd{BjgSCobIXvdoV{[2WwY3WgdI9t[XKrenH0bY9vKGG\|c3H5	M1jYdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkCzOVQxLz5zN{[wN\|U1ODxxYU6=
NCI-H526	M2TZSmZ2dmO2aX;uJIF{e2G7	M3PDcFEhfU1?	NGfvSGM6PiCqcoO=	MWDJcohq[mm2aX;uJI9nKEiVUEmwMY1m\GmjdHXkJJBzd2yrZnXyZZRqd25ib3[gbJVu[W5iTlPJMWg2OjZiY3XscJMh[XRiMTD1UUBi\nSncjC5OkBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigQ>?	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZyM{W0NEc,OTd4MEO1OFA9N2F-
AGS	MlvUSpVv[3Srb36gZZN{[Xl?			MkfETY5pcWKrdHnvckBw\iCqeYDvfIliNWmwZIXj[YQhUEmIMTDhZ5RqfmG2aX;uJIlvKGi3bXHuJGFIWyClZXzsd{BjgSC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR\|UxRTBwMECzOu69VS5?	NYDRS2J7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizOVk3OzFpPkG4N\|U6PjNzPD;hQi=>
HeLa	NX\xPVhOTnWwY4Tpc44h[XO\|YYm=			NVKxSWdmUW6qaXLpeIlwdiCxZjDUUmYu[WyyaHGtbY5lfWOnZDDOSk1s[XCyYVKgZYN1cX[jdHnvckBqdiCqdX3hckBJ\UyjIHPlcIx{NCCLQ{WwQVAvOTYQvF2u	NH\mXWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEO1PVY{OSd-MUizOVk3OzF:L3G+
HeLa	NVnneZN7S3m2b4TvfIlkcXS7IHHzd4F6			NV;4TXc5S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWOYTDj[YxteyCkeTDNWHQh[XO\|YYmsJGlEPTB;Mj6wOu69VS5?	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN3OU[zNUc,OTh\|NUm2N\|E9N2F-
AGS	NWnrR2xQS3m2b4TvfIlkcXS7IHHzd4F6			MlfKR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRWdUKGOnbHzzJIJ6KE2WVDDhd5NigSxiSVO1NF0yPs7:TT6=	NIjFXYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEO1PVY{OSd-MUizOVk3OzF:L3G+
A2058	MVjDfZRwfG:6aXPpeJkh[XO\|YYm=			MVPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBNlA2QCClZXzsd{BjgSCPVGSgZZN{[XluIFnDOVA:OC5yMEKx{txONg>?	M1npTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OUK5OFg3Lz5zOEmyPVQ5PjxxYU6=
MDA-MB-231	MkXISpVv[3Srb36gZZN{[Xl?			MUHJcohq[mm2aX;uJI9nKEi\|cEmwJIlvKGi3bXHuJG1FSS2PQj2yN\|Eh[2WubIOgZZN{\XO\|ZXSgZZMhcGW{MjDk[Ydz[WSjdHnvckwhUUN3ME2wMlAxPDYQvF2u	NHPjUHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEmyPVQ5Pid-MUi5Nlk1QDZ:L3G+
MDA-MB-231	M2jBOWN6fG:2b4jpZ4l1gSCjc4PhfS=>			M3HsdmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN\|Eh[2WubIOgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvODB3ON88UU4>	NFT4RnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEmyPVQ5Pid-MUi5Nlk1QDZ:L3G+
A2058	MljpSpVv[3Srb36gZZN{[Xl?			Mke3TY5pcWKrdHnvckBw\iCKc4C5NEBqdiCqdX3hckBCOjB3ODDj[YxteyxiRVO1NF0xNjByN{pOwG0v	MlTrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh7Mkm0PFYoRjF6OUK5OFg3RC:jPh?=
MDA-MB-231	NWrFWoM1TnWwY4Tpc44h[XO\|YYm=			NE\aT4FKdmirYnn0bY9vKG:oIFjzdFkxKGmwIHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[XO\|ZYPz[YQh[XNiQXv0JIRm\3KjZHH0bY9vNCCLQ{WwQVAvODF5Nt88UU4>	M3HUWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OUK5OFg3Lz5zOEmyPVQ5PjxxYU6=
A2058	MVXGeY5kfGmxbjDhd5NigQ>?			M{SzNGlvcGmkaYTpc44hd2ZiSIPwPVAhcW5iaIXtZY4hSTJyNUigZ4VtdHNiYYPz[ZN{\WRiYYOgRYt1KGSnZ4Lh[IF1cW:wLDDJR\|UxRTBwMEK0N:69VS5?	NXzVZY9qRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi5Nlk1QDZpPkG4PVI6PDh4PD;hQi=>
HuH7	NYP2NHN4SW62aY\pdoFtKGG\|c3H5		NEW1TmY{KGSjeYO=	NVz4cldWSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUGWyYYTpeIl{KENidnnyeZMh\2Wwb4T5dIUhOWJiQ3;uNUBqdm[nY4Tl[EBqdiCqdX3hckBJfUh5IHPlcIx{KGG\|c3Xzd4VlKGG\|IFfBVGRJKFKQQTDvdkAyQFNicmLORUBt\X[nbDDh[pRmeiB\|IHThfZMh[nlicWLUMXBEWiCjbnHsfZNqeyxiRVO1NF0xNjByMUNOwG0v	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDl\|NkG5NUc,OTh7M{[xPVE9N2F-
HuH7	MUnBcpRqfmm{YXygZZN{[Xl?		M2TZT\|Mh\GG7cx?=	NUH2e4N1SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUGWyYYTpeIl{KENidnnyeZMh\2Wwb4T5dIUhOWJiQ3;uNUBqdm[nY4Tl[EBqdiCqdX3hckBJfUh5IHPlcIx{KGG\|c3Xzd4VlKGG\|IFfBVGRJKFKQQTDvdkAyQFNicmLORUBt\X[nbDDh[pRmeiB\|IHThfZMhe2WuZXP0[YQhf2m2aDC0NEBvVSCKQ2[tO\|k3KGGwZDC4NFAhdk1iYn;j[ZBz\X[rcjDifUByWlRvUFPSJIFv[Wy7c3nzMEBGSzVyPUCuNFA{Oc7:TT6=	NVn1NZF4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi5N\|YyQTFpPkG4PVM3OTlzPD;hQi=>
SKBR3	MnXoSpVv[3Srb36gZZN{[Xl?			MX;CbY5lcW6pIHHm[olvcXS7IITvJGh{eDlyIHnuJIh2dWGwIGPLRnI{KGOnbHzzMEBKSzVyPUCuNFI1\|ryPLh?=	NYfoTW5XRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwNVc2PjJpPkG5NFE4PTZ{PD;hQi=>
Hep3B	M1fvWmZ2dmO2aX;uJIF{e2G7		M1q4OVMxKG2rboO=	NHvmRnhKdmirYnn0bY9vKG:oIHj5dI95cWFvaX7keYNm\CCKSV[xZYxxcGFicILveIVqdiCjY3P1cZVt[XSrb36gbY4hcHWvYX6gTIVxO0JiY3XscJMhfHKnYYTl[EBnd3JiM{CgcYlveyCvZXHzeZJm\CCjZoTldkAyOiCqcoOgZpkhX2W\|dHXyckBjdG:2IHHuZYx6e2m\|LDDJR\|UxRTBwMEW3Nu69VS5?	NHvlO\|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUC3NlIyPCd-MUmwO\|IzOTR:L3G+
Hep3B	NVTEdlhSTnWwY4Tpc44h[XO\|YYm=		NGOx[mQyPiCqcoO=	MVXJcohq[mm2aX;uJI9nKGi7cH;4bYEucW6mdXPl[EBXTUeIIIDyc5RmcW5ic3XjdoV1cW:wIHnuJIh2dWGwIFjldFNDKGOnbHzzJIFnfGW{IEG2JIhzeyCkeTDFUGlUSSxiSVO1NF0xNjB5OUZOwG0v	M1r0OFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEeyNlE1Lz5zOUC3NlIyPDxxYU6=
HCT116	NIPV[5ZEgXSxdH;4bYNqfHliYYPzZZk>		NXr2VFI5PzJiaILz	M4DNZmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDFzNjDj[YxteyCjZoTldkA4OiCqcoOsJGlEPTB;MD6wOVfPxE1w	M320dFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MkOxPFY1Lz5zOUKzNVg3PDxxYU6=
SKBR3	MVXDfZRwfG:6aXPpeJkh[XO\|YYm=		M2my[VczKGi{cx?=	NEP5VVNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUU0KUMzDj[YxteyCjZoTldkA4OiCqcoOsJGlEPTB;MD6wOVjPxE1w	MmXOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl{M{G4OlQoRjF7MkOxPFY1RC:jPh?=
MCF7	MXTDfZRwfG:6aXPpeJkh[XO\|YYm=		NWrYNJZ[PzJiaILz	MUTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyxiSVO1NF0xNjB5Md88UU4>	NXnXVJFPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmyN\|E5PjRpPkG5NlMyQDZ2PD;hQi=>
SKOV3	Mo\hR5l1d3SxeHnjbZR6KGG\|c3H5		NYnhemJOPzJiaILz	MVXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT29XOyClZXzsd{Bi\nSncjC3NkBpenNuIFnDOVA:OC5zMkNOwG0v	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTJ\|MUi2OEc,OTl{M{G4OlQ9N2F-
SKBR3	MWXDfZRwfG:6aXPpeJkh[XO\|YYm=		MYG3NkBpenN?	NX:4S5NnS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0uEUkOgZ4VtdHNiYX\0[ZIhPzJiaILzJIlvKHC{ZYPlcoNmKG:oIF7RU\|EhcW6qaXLpeI9zKGSrY3;1cYFzd2xuIFnDOVA:OC5{M988UU4>	NEXMVJE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUKzNVg3PCd-MUmyN\|E5PjR:L3G+
MCF7	MWHDfZRwfG:6aXPpeJkh[XO\|YYm=		NGPUWYs4OiCqcoO=	MYTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCrbjDwdoV{\W6lZTDv[kBPWU9zIHnubIljcXSxcjDkbYNwfW2jcn;sMEBKSzVyPUCuPFYz\|ryPLh?=	M2m1RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MkOxPFY1Lz5zOUKzNVg3PDxxYU6=
NCI-H596	MlP5R5l1d3SxeHnjbZR6KGG\|c3H5		NF;JPWo4OiCqcoO=	NEDYdlBEgXSxdH;4bYNqfHliYXfhbY5{fCCQUUCxMYRm\mmlaXXueEBpfW2jbjDOR2kuUDV7NjDj[YxteyCjZoTldkA4OiCqcoOsJGlEPTB;MT6x{txONg>?	MlTJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl{M{G4OlQoRjF7MkOxPFY1RC:jPh?=
MDA468	MUXDfZRwfG:6aXPpeJkh[XO\|YYm=		M2q5TVczKGi{cx?=	Mk\wR5l1d3SxeHnjbZR6KGGpYXnud5QhVlFyMT3k[YZq[2mnboSgbJVu[W5iTVTBOFY5KGOnbHzzJIFnfGW{IEeyJIhzeyxiSVO1NF0yNjcQvF2u	MmXEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl{M{G4OlQoRjF7MkOxPFY1RC:jPh?=
SKBR3	NGjjRlVEgXSxdH;4bYNqfHliYYPzZZk>		MXi3NkBpenN?	MkPqR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2tDWjNiY3XscJMh[W[2ZYKgO\|IhcHK\|IHL5JINmdGy2aYTldk1odG9iYYPzZZktKEmFNUC9NE4xOjUQvF2u	M4PmNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NEC1OVI5Lz5zOUSwOVUzQDxxYU6=
A549	MWLDfZRwfG:6aXPpeJkh[XO\|YYm=		NWnDfWJpPzJiaILz	NXPxZ2ZHS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA4OiCqcoOgZpkh[2WubITpeIVzNWeubzDhd5NigSxiSVO1NF0xNjB4ON88UU4>	NHTUW4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUSwOVUzQCd-MUm0NFU2Ojh:L3G+
SKOV3	NH7kSldEgXSxdH;4bYNqfHliYYPzZZk>		M2q2SFczKGi{cx?=	M2[ySGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNMV1Z\|IHPlcIx{KGGodHXyJFczKGi{czDifUBk\WyudHn0[ZIu\2yxIHHzd4F6NCCLQ{WwQVAvOjMQvF2u	M2rpdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NEC1OVI5Lz5zOUSwOVUzQDxxYU6=
MCF7	NHixfnFEgXSxdH;4bYNqfHliYYPzZZk>		NEPVelU4OiCqcoO=	NWjTXHYzS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkA4OiCqcoOgZpkh[2WubITpeIVzNWeubzDhd5NigSxiSVO1NF0xNjJ\|zszNMi=>	NXL2O4ZMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0NFU2OjhpPkG5OFA2PTJ6PD;hQi=>
CCRF-CEM	M37v[GN6fG:2b4jpZ4l1gSCjc4PhfS=>		NFfzdI04OiCqcoO=	Ml\mR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gR2NTTi2FRV2gZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGOnbHz0bZRmei17NjDhdZVmd3W\|IH;u[UB{d2y3dHnvckBie3OjeTygTWM2OD1yLkW0{txONg>?	MlrqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2MEW1NlgoRjF7NEC1OVI5RC:jPh?=
CCRF-CEM	MVfDfZRwfG:6aXPpeJkh[XO\|YYm=		MY[3NkBpenN?	NYjYTWpPS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4heGGlbHn0ZZhmdC2{ZYPpd5RidnRiQ1PSSk1ETU1iY3XscJMh[W[2ZYKgO\|IhcHK\|IHL5JINmdGy2aYTldk06PiCjcYXlc5V{KG:wZTDzc4x2fGmxbjDhd5NigSxiSVO1NF0zNjYQvF2u	MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTRyNUWyPEc,OTl2MEW1Nlg9N2F-
Hep3B	NWH4VnlITnWwY4Tpc44h[XO\|YYm=		NUW3WXh{OzBibXnudy=>	MnfQTY5pcWKrdHnvckBw\iCqeYDvfIliNWmwZIXj[YQhUEmIMXHsdIhiKHC{b4TlbY4h[WOldX31cIF1cW:wIHnuJIh2dWGwIFjldFNDKGOnbHzzJJRz\WG2ZXSg[o9zKDNyIH3pcpMhdWWjc4Xy[YQh[W[2ZYKgNVIhcHK\|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqeyxiSVO1NF0xNjB3N988UU4>	Mmn3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2Nkm4PFcoRjJyNE[5PFg4RC:jPh?=
Hep3B	M2WwOmZ2dmO2aX;uJIF{e2G7		NWLxWWlFOTZiaILz	MXTJcohq[mm2aX;uJI9nKGi7cH;4bYEucW6mdXPl[EBXTUeIIIDyc5RmcW5ic3XjdoV1cW:wIHnuJIh2dWGwIFjldFNDKGOnbHzzJIFnfGW{IEG2JIhzeyCkeTDFUGlUSSxiSVO1NF0xNjB5Od88UU4>	NGrDdHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES2PVg5Pyd-MkC0Olk5QDd:L3G+
HCT116	M{DOXWN6fG:2b4jpZ4l1gSCjc4PhfS=>			NWfQWpVOS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWMUG2JINmdGy\|IHL5JGFt[W2jcjDicJVmKGG\|c3H5MEBKSzVyPUCuNFXPxE1w	M33TclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNk[yOVM1Lz5{ME[2NlU{PDxxYU6=
NCI-H1299	NUXFc2hTTnWwY4Tpc44h[XO\|YYm=		M{mwOVI1KGi{cx?=	NHu4NVNKdmirYnn0bY9vKG:oIHj1cYFvKEiVUEmwJIlvKGi3bXHuJG5EUS2KMUK5PUBk\WyuczDhd5Nme3OnZDDhd{BCc3RiZHXndoFl[XSrb36gZYZ1\XJiMkSgbJJ{KGK7IHz1cYlv\XhiYYPzZZktKEmFNUC9NE4y\|ryPLh?=	MnLPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2M{i1OFEoRjJzNEO4OVQyRC:jPh?=
LN229-Lux	NIDNc3dHfW6ldHnvckBie3OjeR?=	M3LyVlIvPSC2bzCxNEB2VQ>?	MonINUBpeg>?	MoDpTY5pcWKrdHnvckBw\iCudXPp[oVz[XOnIHHjeIl3cXS7IHnuJIh2dWGwIFzONlI6NUy3eDDj[YxteyCjdDCyMlUhfG9iMUCgeW0hcW6ldXLheIVlKG[xcjCxJIhzKHWwZHXyJI5wem2xeHnhJIZwdGyxd3XkJIJ6KDJ2IHjyd{B2dmSncjDofZBwgGmjIHL5JJJmeG:{dHXyJIdmdmViYYPzZZk>	M{DVXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{S2Nlc1Lz5{Mke0OlI4PDxxYU6=
MCF7	NWTtNphsSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NI[xc2g1QCCqcoO=	Ml\1RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPQ1[3JINmdGy\|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK\|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlM6\|ryPLh?=	NVfobpBqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1PFI1PzdpPkK0OVgzPDd5PD;hQi=>
HCT116	Ml\HRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		M2GxUVQ5KGi{cx?=	MoL4RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCKQ2SxNVYh[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvPzkQvF2u	NFPwTZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEW4NlQ4Pyd-MkS1PFI1Pzd:L3G+
SKBR3	NIDKepFCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		MXy0PEBpenN?	M1LaXGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1vCVlMh[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVEvOzUQvF2u	NUjaXJB1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1PFI1PzdpPkK0OVgzPDd5PD;hQi=>
A231	MULBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		Mmf4OFghcHK\|	MkXtRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCDMkOxJINmdGy\|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OC5zN988UU4>	M{\uXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{[zNlYyLz5{NEe2N\|I3OTxxYU6=
MCF7	NFrOOItCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NHT2T441QCCqcoO=	MkGyRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPQ1[3JINmdGy\|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OC56zszNMi=>	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd4M{K2NUc,OjR5NkOyOlE9N2F-
HCT116	MV7BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NU\ONGtVPDhiaILz	M3S0fWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFPUNVE3KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MT6yNe69VS5?	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd4M{K2NUc,OjR5NkOyOlE9N2F-
SKBR3	NGfjXWVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		MkHnOFghcHK\|	M4HWRWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1vCVlMh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1\|LkGx{txONg>?	M{fDN\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{[zNlYyLz5{NEe2N\|I3OTxxYU6=
NCI-H1299	NELQSHRHfW6ldHnvckBie3OjeR?=		MYKxNkBpenN?	M1;KS3Jm\HWldHnvckBqdiCxeInn[Y4h[2:wc4XtdJRqd25icnH0[UBqdiCqdX3hckBPS0lvSEGyPVkh[2WubIOgbY5kfWKjdHXkJIZweiBzMjDodpM>	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN6M{mxOUc,OjV\|OEO5NVU9N2F-
PC9	NYXwNY5zS3m2b4TvfIlkcXS7IHHzd4F6		NWmxVFRLPzJiaILz	MYHDfZRwfG:6aXPpeJkh[WejaX7zeEBJT0ZvaX7keYNm\CCncnzveIlvcWJvcnXzbZN1[W62IHj1cYFvKFCFOTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGOnbHyg[5Jwf3SqIHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OC5yMd88UU4>	M3f3NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OES0Olg6Lz5{Nki0OFY5QTxxYU6=
Ma1	NUjP[HExS3m2b4TvfIlkcXS7IHHzd4F6		M3vqS\|czKGi{cx?=	NGCz[lZEgXSxdH;4bYNqfHliYXfhbY5{fCCKR1[tbY5lfWOnZDDldoxwfGmwaXKtdoV{cXO2YX70JIh2dWGwIF3hNUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHPlcIwh\3Kxd4ToJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MD6wNe69VS5?	NYjkfHhNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[4OFQ3QDlpPkK2PFQ1Pjh7PD;hQi=>
SKBR3	NE\wWGxHfW6ldHnvckBie3OjeR?=			M{HkRWlvcGmkaYTpc44hd2ZiSIPwPVAhcW5iaIXtZY4hW0uEUkOgZ4VtdHNuIFnDOVA:OC5yMkVOwG0v	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjh2NE[4PUc,OjZ6NES2PFk9N2F-
HeLa	NYDrVZRmTnWwY4Tpc44h[XO\|YYm=	M1eyOFExKHWP	M3ezblYhcHK\|	M{TvTGlvcGmkaYTpc44hd2ZiSGPQPVAhcW5iaIXtZY4hUGWOYTDj[YxteyCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4Yh[2itMTDk[Ydz[WSjdHnvckBifCBzMDD1UUBi\nSncjC2JIhzeyCkeTDX[ZN1\XKwIHLsc5QhdWW2aH;k	M{Hn[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OEG2OFQ6Lz5{OEixOlQ1QTxxYU6=
HeLa	MVjGeY5kfGmxbjDhd5NigQ>?	M33IdlExKHWP	Ml;ROkBpenN?	M{i0ZmlvcGmkaYTpc44hd2ZiSGPQPVAhcW5iaIXtZY4hUGWOYTDj[YxteyCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4YhSWu2IHTl[5Ji\GG2aX;uJIF1KDFyIIXNJIFnfGW{IE[gbJJ{KGK7IGfld5Rmem5iYnzveEBu\XSqb3S=	NUfDXZNwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki4NVY1PDlpPkK4PFE3PDR7PD;hQi=>
HeLa	MVfGeY5kfGmxbjDhd5NigQ>?	MmfENVAhfU1?	NXTROWpLPiCqcoO=	NIHKb3NKdmirYnn0bY9vKG:oIFjTVFkxKGmwIHj1cYFvKEinTHGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5lfWO2aX;uJI9nKEiVUEewJJBzd3SnaX6g[ZhxemW\|c3nvckBifCBzMDD1UUBi\nSncjC2JIhzeyCkeTDX[ZN1\XKwIHLsc5QhdWW2aH;k	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDhzNkS0PUc,Ojh6MU[0OFk9N2F-
PC3	MnXYSpVv[3Srb36gZZN{[Xl?	MmLGNVAhfU1?	NX22Rm51PiCqcoO=	M1jXfmlvcGmkaYTpc44hd2ZiSGPQPVAhcW5iaIXtZY4hWEN\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnu[JVkfGmxbjDv[kBkcGtzIHTl[5Ji\GG2aX;uJIF1KDFyIIXNJIFnfGW{IE[gbJJ{KGK7IGfld5Rmem5iYnzveEBu\XSqb3S=	MkjiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh6MU[0OFkoRjJ6OEG2OFQ6RC:jPh?=
PC3	MnHCSpVv[3Srb36gZZN{[Xl?	MXOxNEB2VQ>?	MlTqOkBpenN?	NX6z[GFjUW6qaXLpeIlwdiCxZjDIV3A6OCCrbjDoeY1idiCSQ{OgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5lfWO2aX;uJI9nKEGtdDDk[Ydz[WSjdHnvckBifCBzMDD1UUBi\nSncjC2JIhzeyCkeTDX[ZN1\XKwIHLsc5QhdWW2aH;k	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDhzNkS0PUc,Ojh6MU[0OFk9N2F-
PC3	NUDiPGZmTnWwY4Tpc44h[XO\|YYm=	NFXPe4wyOCC3TR?=	MVG2JIhzew>?	NYXQTHlzUW6qaXLpeIlwdiCxZjDIV3A6OCCrbjDoeY1idiCSQ{OgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5lfWO2aX;uJI9nKEiVUEewJJBzd3SnaX6g[ZhxemW\|c3nvckBifCBzMDD1UUBi\nSncjC2JIhzeyCkeTDX[ZN1\XKwIHLsc5QhdWW2aH;k	MnzKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh6MU[0OFkoRjJ6OEG2OFQ6RC:jPh?=

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	HSP90 / HSP70 ; PubMed: 28915605 Oncotarget Western blot analysis showing dose-dependent elevation of Hsp70 and HSP90 levels in AGS cells by 17-DMAG. p-Akt / Survivin / MMP2 ; PubMed: 28915605 Oncotarget Western blot analysis showing the dose-dependent reduction of the expression of HSP90 client proteins, such as p-Akt, survivin, and MMP2 by 17-DMAG. PARP / Cleaved caspase-3 / Cleaved caspase-8 / Cleaved caspase-9 / PUMA ; PubMed: 28915605 Oncotarget (C-D) 17-DMAG effects on the expression of apoptotic proteins (PARP, c-caspase 3, c-caspase-8, c-caspase-9, and PUMA) in AGS cells. Western blot analyses indicate that 17-DMAG significantly increased the expression of apoptotic proteins in AGS cells in a dose- (C) and time- (D) dependent manner (P < 0.05). p-ALK / ALK / p-Akt / Akt / p-ERK / ERK ; PubMed: 26219569 BMC Cancer Modulation of ALK signaling in parental and 17-DMAG-resistant cells. Cells were treated with the indicated concentrations of 17-DMAG or AUY922 for 6 h. The molecules of ALK-related signaling activity were detected by western blot analysis. α-Tax / α-IKKα / α-IKKβ/ α-NEMO / α-TBK1 / α-p65 / α-p50 ; PubMed: 24109220 J Virol C8166, MT-2, and MT-4 cells were treated with 17-DMAG as indicated, and cell lysates were subjected to immunoblotting with the indicated antibodies.	28915605 26219569 24109220
Growth inhibition assay	Cell proliferation ; PubMed: 28915605 Oncotarget Proliferation assay of AGS cells treated with graded concentrations of 17-DMAG for 24 h or 48 h. 17-DMAG resulted in significant dose- and time-dependent reduction of AGS cell proliferation (P < 0.05)	28915605

In vivo

17-DMAG treatment at 5 mg/kg or 25 mg/kg thrice per week significantly reduces tumor growth of TMK-1 xenografts, by significantly reducing vessel area and numbers of proliferating tumor cells in sections. ^[2] Consistent the inhibition of FAK signaling in vivo, 17-DMAG treatment at 25 mg/kg three times a week significantly suppresses tumor growth, and metastasis of ME180 and SiHa xenografts in mice. ^[4] Administration of 17-DMAG at 10 mg/kg for 16 days significantly decreases the white blood cell count and prolongs the survival in a TCL1-SCID transplant mouse model. ^[5]

Protocol

Kinase Assay:^[1]	- Collapse Fluorescence polarization (FP)-based competition binding assay: This assay utilizes a boron difluoride dipyrromethene (BODIPY) labeled geldanamycin analogue (BODIPY-AG) as a probe and measured fluorescence polarization upon binding of the probe to a protein. Native human Hsp90 protein (α + β isoforms) is isolated from HeLa cells. BODIPY-AG solution is freshly prepared in FP assay buffer (20 mM HEPES-KOH, pH 7.3, 1.0 mM EDTA, 100 mM KCl, 5.0 mM MgCl₂, 0.01% NP-40, 0.1 mg/mL fresh bovine γ-globulin (BGG), 1.0 mM fresh DTT, and protease inhibitor from stock solution in DMSO. Competition curves are obtained by mixing 10 μL each of a solution containing BODIPY-AG and Hsp90, and a serial dilution of 17-DMAG freshly prepared in FP assay buffer from stock solution in DMSO. Final concentrations are 10 nM BODIPY-AG, 40 or 60 nM Hsp90, varying concentration of 17-DMAG (0.10 nM-10 μM), and ≤0.25% DMSO in a 384-well microplate. After 3 hours incubation at 30 °C, fluorescence anisotropy (γ_Ex = 485 nm, γ_Em = 535 nm) is measured on an EnVision 2100 multilabel plate reader. IC50 value of 17-DMAG is obtained from the competition curves.
Cell Research:^[5]	- Collapse Cell lines: Chronic lymphocytic leukemia (CLL) Concentrations: Dissolved in DMSO, final concentrations ~1 μM Incubation Time: 24, or 48 hours Method: Cells are exposed to various concentrations of 17-DMAG for 24, or 48 hours. For the assessment of cytotoxicity, MTT reagent is then added, and plates are incubated for an additional 24 hours before spectrophotometric measurement. Apoptosis is determined by staining with annexin V-fluorescein isothiocyanate and propidium iodide (PI). (Only for Reference)
Animal Research:^[5]	- Collapse Animal Models: SCID mice engrafted with TCL1 leukemia cells Dosages: 10 mg/kg Administration: Intraperitoneal injection 5 times per week (Only for Reference)

References

- Collapse

Solubility (25°C)

In vitro	DMSO	131 mg/mL (200.54 mM)
	Water	Insoluble
	Ethanol	Insoluble
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 1% DMSO+30% polyethylene glycol+1% Tween 80 For best results, use promptly after mixing.	30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Alvespimycin (17-DMAG) HCl SDF

Molecular Weight	653.21
Formula	C₃₂H₄₈N₄O₈•HCl
CAS No.	467214-21-7
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	NSC 707545,BMS 826476 HCl,KOS 1022
Smiles	CC1CC(C(C(C=C(C(C(C=CC=C(C(=O)NC2=CC(=O)C(=C(C1)C2=O)NCCN(C)C)C)OC)OC(=O)N)C)C)O)OC.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)

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Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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The Serial Dilution Calculator Equation

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Computed Result

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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HSP (HSP90) Signaling Pathway Map

HSP (HSP90) Inhibitors with Unique Features

Selective HSP90 Inhibitor

NVP-BEP800 : HSP90β-selective, IC50=58 nM.
Most Potent HSP90 Inhibitor

KW-2478 : HSP90, IC50=3.8 nM.
HSP90 Inhibitor in Clinical Trial

17-AAG (Tanespimycin) : Phase III for Gastrointestinal Stromal Tumors.
Newest HSP90 Inhibitor

XL888 : ATP-competitive inhibitor of HSP90 with IC50 of 24 nM.

Related HSP (HSP90) Products

PU-H71 ^New

PU-H71 (NSC 750424) is a potent and selective inhibitor of HSP90 with IC50 of 51 nM. Phase 1.
KNK437 ^New

KNK437 is a pan-HSP inhibitor, which inhibits the synthesis of inducible HSPs, including HSP105, HSP72, and HSP40.
VER155008 ^New

VER155008 (C07) is a potent Hsp70 family inhibitor with IC50 of 0.5 μM, 2.6 μM, and 2.6 μM in cell-free assays for HSP70, HSC70, and GRP78 (HSPA5, Bip), respectively, >100-fold selectivity over HSP90. VER155008 inhibits autophagy and causes reduced levels of HSP90 client proteins.
Tanespimycin (17-AAG)

Tanespimycin (17-AAG, CP127374, NSC-330507, KOS 953) is a potent HSP90 inhibitor with IC50 of 5 nM in a cell-free assay, having a 100-fold higher binding affinity for HSP90 derived from tumour cells than HSP90 from normal cells. Tanespimycin (17-AAG) induces apoptosis, necrosis, autophagy and mitophagy. Phase 3.

Features:Displays very low toxicity toward normal cells.
Luminespib (NVP-AUY922)

Luminespib (AUY-922, NVP-AUY922, VER-52296) is a highly potent HSP90 inhibitor for HSP90α/β with IC50 of 13 nM /21 nM in cell-free assays, weaker potency against the HSP90 family members GRP94 and TRAP-1, exhibits the tightest binding of any small-molecule HSP90 ligand. Luminespib (AUY-922, NVP-AUY922) effectively downregulates and destabilizes the IGF-1Rβ protein and results in growth inhibition, autophagy and apoptosis. Phase 2.
Ganetespib (STA-9090)

Ganetespib (STA-9090) is an HSP90 inhibitor with IC50 of 4 nM in OSA 8 cells, induces apoptosis of OSA cells while normal osteoblasts are not affected; active metabolite of STA-1474. Phase 3.
Geldanamycin

Geldanamycin (NSC 122750) is a natural existing HSP90 inhibitor with K_d of 1.2 μM, specifically disrupts glucocorticoid receptor (GR)/HSP association. Geldanamycin attenuates virus infection-induced ALI (acute lung injury)/ARDS (acute respiratory distress syndrome) by reducing the host's inflammatory responses.
HSP990 (NVP-HSP990)

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Features:NVP-HSP990 is an orally available HSP90 inhibitor and is structurally distinct from other clinical HSP90 inhibitors.
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Alvespimycin (17-DMAG) HCl