Alvespimycin (17-DMAG) HCl

Name: Alvespimycin (17-DMAG) HCl
Brand: Selleck Chemicals
SKU: S1142
Rating: 5 (50 reviews)

For research use only.

Catalog No.S1142 Synonyms: NSC 707545,BMS 826476 HCl,KOS 1022

50 publications

Alvespimycin (17-DMAG) HCl Chemical Structure

CAS No. 467214-21-7

Alvespimycin (17-DMAG, NSC 707545, BMS 826476, KOS 1022) HCl is a potent HSP90 inhibitor with IC50 of 62 nM in a cell-free assay. Phase 2.

Selleck's Alvespimycin (17-DMAG) HCl has been cited by 50 publications

Sci Adv, 2021, 7(7)eabd2645

PubMed: 33579708 ( click the link to review the publication )

J Virol, 2021, JVI0059421

PubMed: 34037421 ( click the link to review the publication )

Transl Oncol, 2021, 14(1):100982

PubMed: 33395748 ( click the link to review the publication )

Nat Commun, 2020, 22;11(1):1935

PubMed: 32321921 ( click the link to review the publication )

Aging Cell, 2020, e13269

PubMed: 33145977 ( click the link to review the publication )

Cancers (Basel), 2020, 12(11)E3335

PubMed: 33187254 ( click the link to review the publication )

Cancers (Basel), 2020, 12(9)E2630

PubMed: 32942617 ( click the link to review the publication )

J Mol Cell Biol, 2020, 24;12(3):216-229

PubMed: 31408169 ( click the link to review the publication )

Hum Mutat, 2020, 10.1002/humu.24147

PubMed: 33252173 ( click the link to review the publication )

Front Oncol, 2020, 10:117

PubMed: 32117764 ( click the link to review the publication )

Life Sci, 2020, 15;249:117532

PubMed: 32151689 ( click the link to review the publication )

J Bioenerg Biomembr, 2020, 15

PubMed: 32291605 ( click the link to review the publication )

Theranostics, 2020, 10(18):8415-8429

PubMed: 32724478 ( click the link to review the publication )

Yonsei Med J, 2020, 61(7):587-596

PubMed: 32608202 ( click the link to review the publication )

Nat Commun, 2019, 10(1):3562

PubMed: 31395886 ( click the link to review the publication )

Mol Cancer Ther, 2019, 18(11):2097-2110

PubMed: 31395684 ( click the link to review the publication )

Mol Pharm, 2019, 16(5):2069-2082

PubMed: 30916978 ( click the link to review the publication )

Nan Fang Yi Ke Da Xue Xue Bao, 2019, 39(2):169-174

PubMed: 30890504 ( click the link to review the publication )

Hepatology, 2019, 69(2):573-586

PubMed: 29356025 ( click the link to review the publication )

Nat Commun, 2018, 9(1):4296

PubMed: 30327466 ( click the link to review the publication )

Nat Commun, 2018, 9(1):672

PubMed: 29445180 ( click the link to review the publication )

Mol Syst Biol, 2018, 14(3):e7858

PubMed: 29507054 ( click the link to review the publication )

Biomed Pharmacother, 2018, 105:1026-1032

PubMed: 30021337 ( click the link to review the publication )

Nat Commun, 2017, 8(1):422

PubMed: 28871086 ( click the link to review the publication )
Mol Cancer Ther, 2017,

PubMed: 28619760 ( click the link to review the publication )

Int J Biol Sci, 2017, 13(4):505-517

PubMed: 28529458 ( click the link to review the publication )

Experimental Cell Research, 2017, 353(1):46-53

PubMed: 28279658 ( click the link to review the publication )

Am J Transl Res, 2017, 9(11):4945-4953

PubMed: 29218092 ( click the link to review the publication )

Cell Death Dis, 2016, 7(6):e2290

PubMed: 27362807 ( click the link to review the publication )

Sci Rep, 2016, 6:19004

PubMed: 26743233 ( click the link to review the publication )

Sci Rep, 2016, 6:38072

PubMed: 27909289 ( click the link to review the publication )

J Biol Chem, 2016,

PubMed: 27489107 ( click the link to review the publication )

Cell Stress Chaperones, 2016, 21(2):339-48

PubMed: 26786409 ( click the link to review the publication )

Medicinal Chemistry Research, 2016, 10.1007/s00044-016-1553-7

PubMed: ( click the link to review the publication )

Viruses, 2016, 8(11)

PubMed: 27801778 ( click the link to review the publication )

Oncotarget, 2016, 7(1):433-45

PubMed: 26595521 ( click the link to review the publication )

Molecules, 2015, 20(1):1643-60

PubMed: 25608045 ( click the link to review the publication )

Immunopharmacol Immunotoxicol, 2015, 8:1-10

PubMed: 26642940 ( click the link to review the publication )

Oncotarget, 2015, 6(14):12128-40

PubMed: 25900240 ( click the link to review the publication )

PLoS One, 2014, 9(12):e114000

PubMed: 25486409 ( click the link to review the publication )

Oncotarget, 2014, 5(13):4920-8

PubMed: 24952482 ( click the link to review the publication )

Zhongguo Fei Ai Za Zhi, 2014, 17(11):778-82

PubMed: 25404267 ( click the link to review the publication )

Assay Drug Dev Technol, 2014, 12(9-10):514-26

PubMed: 25506801 ( click the link to review the publication )

Hepatology, 2013, 57(1):70-80

PubMed: 22898980 ( click the link to review the publication )

Mol Oncol, 2013, 7(6):1093-102

PubMed: 23993685 ( click the link to review the publication )

FEBS J, 2013, 10.1111/febs.12672

PubMed: 24304935 ( click the link to review the publication )

PLoS One, 2013, 8(4):e59315

PubMed: 23565147 ( click the link to review the publication )

Tumour Biol, 2013, 35(4):3229-35

PubMed: 24293393 ( click the link to review the publication )
J Thorac Oncol, 2012, 7(7):1078-85

PubMed: 22592212 ( click the link to review the publication )

Eukaryot Cell, 2012, 11(11):1324-32

PubMed: 22822234 ( click the link to review the publication )

Purity & Quality Control

Choose Selective HSP (HSP90) Inhibitors

Biological Activity

Description

Alvespimycin (17-DMAG, NSC 707545, BMS 826476, KOS 1022) HCl is a potent HSP90 inhibitor with IC50 of 62 nM in a cell-free assay. Phase 2.

Features

A synthetic derivative Geldanamycin, with lower hepatotoxicity than parent antibiotic & higher potency and bioavailability than the similar derivative 17-AAG.

Targets

HSP90 ^[1]
(Cell-free assay)

62 nM

In vitro

17-DMAG displays ~2 times potency against human Hsp90 than 17-AAG, with IC50 of 62 nM versus 119 nM. In SKBR3 and SKOV3 cells which over-express Hsp90 client protein Her2, 17-DMAG causes down-regulation of Her2 with EC50 of 8 nM and 46 nM, respectively, as well as induction of Hsp70 with EC50 of 4 nM and 14 nM, respectively, leading to significant cytotoxicity with GI50 of 29 nM and 32 nM, respectively, consistent with Hsp90 inhibition. ^[1] 17-DMAG in combination with vorinostat synergistically induces apoptosis of the cultured MCL cells as well as primary MCL cells, more potently than either agent alone, by markedly attenuating the levels of cyclin D1 and CDK4, as well as of c-Myc, c-RAF and Akt. ^[3] In contrast to 17-AAG which is only active for IKKβ in chronic lymphocytic leukemia (CLL) cells, 17-DMAG treatment effectively leads to depletion of the Hsp90 client protein, resulting in diminished NF-κB p50/p65 DNA binding, decreased NF-κB target gene transcription, and caspase-dependent apoptosis. By targeting the NF-κB family, 17-DMAG selectively mediates dose- and time-dependent cytotoxicity against CLL cells, but not normal T cells or NK cells important for immune surveillance. ^[5]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
SKBr3	NXHMcHU2S3m2b4TvfIlkcXS7IHHzd4F6			NIfCOFFEgXSxdH;4bYNqfHliYXfhbY5{fCCVS1LyN{Bk\WyuczygTWM2OD1yLkCyOO69VS5?	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjF4NUO1OEc,OTZzNkWzOVQ9N2F-
SKBR3	NEHTeIdHfW6ldHnvckBie3OjeR?=			NFTmV4hWeHKnZ4XsZZRqd25ib3[gTJNxPzBiaX6gV2tDWjNiY3XscJMtKEWFNUC9NE4xODUQvF2u	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjh3NEC2Okc,OTZ6NUSwOlY9N2F-
SKBR3	M3S5fGZ2dmO2aX;uJIF{e2G7			MXrE[Ydz[WSjdHnvckBw\iCKZYKyJIlvKFONQmKzJINmdGy\|LDDFR\|UxRTBwMEC4{txONg>?	NID2OpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNki1OFA3Pid-MU[4OVQxPjZ:L3G+
SKOV3	NXz6bGVRTnWwY4Tpc44h[XO\|YYm=			MYjVdJJm\3WuYYTpc44hd2ZiSIPwO\|AhcW5iU1vPWlMh[2WubIOsJGVEPTB;MD6wNVTPxE1w	M3nLSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OEW0NFY3Lz5zNki1OFA3PjxxYU6=
SKOV3	M1i2SGZ2dmO2aX;uJIF{e2G7			MWTE[Ydz[WSjdHnvckBw\iCKZYKyJIlvKFONT2[zJINmdGy\|LDDFR\|UxRTBwMES2{txONg>?	M17JT\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OEW0NFY3Lz5zNki1OFA3PjxxYU6=
AGS	NWO4NIxjTnWwY4Tpc44h[XO\|YYm=		MVexOkBpenN?	MlzwTY5pcWKrdHnvckBw\iCKSV[xJIFkfGm4YYTpc44hcW5iaIXtZY4hSUeVIHPlcIx{KGG\|c3Xzd4VlKGG\|IHnubIljcXSrb36gc4YhcHmyb4jpZU1qdmS3Y3XkJIx2[2moZYLhd4Uh\XiycnXzd4lwdiCjZoTldkAyPiCqcoOgZpkhemWyb4L0[ZIh[XO\|YYmsJGlEPTB;MD6wN\|bPxE1w	M1rQO\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUizPVUxLz5zN{W4N\|k2ODxxYU6=
Hep3B	MnrCSpVv[3Srb36gZZN{[Xl?		M3PNUlE3KGi{cx?=	NIi2[2hKdmirYnn0bY9vKG:oIFjJSlEh[WO2aY\heIlwdiCrbjDoeY1idiCKZYCzRkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHj5dI95cWFvaX7keYNm\CCudXPp[oVz[XOnIHX4dJJme3Orb36gZYZ1\XJiMU[gbJJ{KGK7IILldI9zfGW{IHHzd4F6NCCLQ{WwQVAvODZzzszNMi=>	NYj2Uow{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PFM6PTBpPkG3OVg{QTVyPD;hQi=>
AGS	NH\JfGFHfW6ldHnvckBie3OjeR?=		M{PX[VI1KGi{cx?=	MnzqWoli[mmuaYT5JI9nKGi3bXHuJGFIWyClZXzsd{B2dmSncjDuc5Jud3irYzDjc45lcXSrb37zJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MUdOwG0v	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV6M{m1NEc,OTd3OEO5OVA9N2F-
NCI-H526	NXzhVFBtTnWwY4Tpc44h[XO\|YYm=	MYOxJJVO	MoToNlQhcHK\|	NULRb2hbSmmwZHnu[{Bi\m[rbnn0fUB1dyCKU2C5NEBqdiCqdX3hckBPS0lvSEWyOkBk\WyuczDheEAyKHWPIHHmeIVzKDJ2IHjyd{BjgSCobIXvdoV{[2WwY3WgdI9t[XKrenH0bY9vKGG\|c3H5	MljKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4MEO1OFAoRjF5NkCzOVQxRC:jPh?=
NCI-H526	M{C3NGZ2dmO2aX;uJIF{e2G7	NF20N3QyKHWP	NVvXTIo4QTZiaILz	NITUeIdKdmirYnn0bY9vKG:oIFjTVFkxNW2nZHnheIVlKHC{b3zp[oVz[XSrb36gc4YhcHWvYX6gUmNKNUh3Mk[gZ4VtdHNiYYSgNUB2VSCjZoTldkA6PiCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBie3OjeR?=	NGH1[I09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[wN\|U1OCd-MUe2NFM2PDB:L3G+
AGS	MUXGeY5kfGmxbjDhd5NigQ>?			MU\Jcohq[mm2aX;uJI9nKGi7cH;4bYEucW6mdXPl[EBJUUZzIHHjeIl3[XSrb36gbY4hcHWvYX6gRWdUKGOnbHzzJIJ6KHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVA:OC5yMEO2{txONg>?	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN3OU[zNUc,OTh\|NUm2N\|E9N2F-
HeLa	Moq2SpVv[3Srb36gZZN{[Xl?			NG\qeWJKdmirYnn0bY9vKG:oIGTOSk1idHCqYT3pcoR2[2WmIF7GMYtieHCjQjDhZ5RqfmG2aX;uJIlvKGi3bXHuJGhmVGFiY3XscJMtKEmFNUC9NE4yPc7:TT6=	M2TiTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{W5OlMyLz5zOEO1PVY{OTxxYU6=
HeLa	MWnDfZRwfG:6aXPpeJkh[XO\|YYm=			MmL3R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{BjgSCPVGSgZZN{[XluIFnDOVA:Oi5yNt88UU4>	MlrhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh\|NUm2N\|EoRjF6M{W5OlMyRC:jPh?=
AGS	NGrpe5VEgXSxdH;4bYNqfHliYYPzZZk>			NGfJXJJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCT1NiY3XscJMh[nliTWTUJIF{e2G7LDDJR\|UxRTF4zszNMi=>	NIX2RWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEO1PVY{OSd-MUizOVk3OzF:L3G+
A2058	NXXjU\|NKS3m2b4TvfIlkcXS7IHHzd4F6			NF7kPHVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCOjB3ODDj[YxteyCkeTDNWHQh[XO\|YYmsJGlEPTB;MD6wNFIy\|ryPLh?=	MkfxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh7Mkm0PFYoRjF6OUK5OFg3RC:jPh?=
MDA-MB-231	MYHGeY5kfGmxbjDhd5NigQ>?			MkGxTY5pcWKrdHnvckBw\iCKc4C5NEBqdiCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIF{e2W\|c3XkJIF{KGinckKg[IVoemGmYYTpc44tKEmFNUC9NE4xODR3zszNMi=>	MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDl{OUS4Okc,OTh7Mkm0PFY9N2F-
MDA-MB-231	NEexTpFEgXSxdH;4bYNqfHliYYPzZZk>			M4jtZmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN\|Eh[2WubIOgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvODB3ON88UU4>	NF\Ue5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEmyPVQ5Pid-MUi5Nlk1QDZ:L3G+
A2058	M2jKd2Z2dmO2aX;uJIF{e2G7			NVTDVpFzUW6qaXLpeIlwdiCxZjDId5A6OCCrbjDoeY1idiCDMkC1PEBk\WyuczygSWM2OD1yLkCwO\|nPxE1w	M2TrPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OUK5OFg3Lz5zOEmyPVQ5PjxxYU6=
MDA-MB-231	NVrwNGZlTnWwY4Tpc44h[XO\|YYm=			M3;3T2lvcGmkaYTpc44hd2ZiSIPwPVAhcW5iaIXtZY4hVUSDLV3CMVI{OSClZXzsd{Bie3Onc4Pl[EBieyCDa4Sg[IVoemGmYYTpc44tKEmFNUC9NE4xOTd4zszNMi=>	Mk\DQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh7Mkm0PFYoRjF6OUK5OFg3RC:jPh?=
A2058	M{PRNmZ2dmO2aX;uJIF{e2G7			NVfwRlZMUW6qaXLpeIlwdiCxZjDId5A6OCCrbjDoeY1idiCDMkC1PEBk\WyuczDhd5Nme3OnZDDhd{BCc3RiZHXndoFl[XSrb36sJGlEPTB;MD6wNlQ{\|ryPLh?=	NIrDdXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEmyPVQ5Pid-MUi5Nlk1QDZ:L3G+
HuH7	MWfBcpRqfmm{YXygZZN{[Xl?		MVqzJIRigXN?	M1jlbmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhmeGG2aYTpd{BEKH[rcoXzJIdmdm:2eYDlJFFjKEOxbkGgbY5n\WO2ZXSgbY4hcHWvYX6gTJVJPyClZXzsd{Bie3Onc4Pl[EBieyCJQWDETEBTVkFib4KgNVhUKHKUTlGgcIV3\WxiYX\0[ZIhOyCmYYnzJIJ6KHGUVD3QR3Ih[W6jbInzbZMtKEWFNUC9NE4xODF{zszNMi=>	NFWwcXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEmzOlE6OSd-MUi5N\|YyQTF:L3G+
HuH7	MWLBcpRqfmm{YXygZZN{[Xl?		MXGzJIRigXN?	NYe1SZo3SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUGWyYYTpeIl{KENidnnyeZMh\2Wwb4T5dIUhOWJiQ3;uNUBqdm[nY4Tl[EBqdiCqdX3hckBJfUh5IHPlcIx{KGG\|c3Xzd4VlKGG\|IFfBVGRJKFKQQTDvdkAyQFNicmLORUBt\X[nbDDh[pRmeiB\|IHThfZMhe2WuZXP0[YQhf2m2aDC0NEBvVSCKQ2[tO\|k3KGGwZDC4NFAhdk1iYn;j[ZBz\X[rcjDifUByWlRvUFPSJIFv[Wy7c3nzMEBGSzVyPUCuNFA{Oc7:TT6=	M3rJ[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OUO2NVkyLz5zOEmzOlE6OTxxYU6=
SKBR3	NVzCSZlzTnWwY4Tpc44h[XO\|YYm=			MXPCbY5lcW6pIHHm[olvcXS7IITvJGh{eDlyIHnuJIh2dWGwIGPLRnI{KGOnbHzzMEBKSzVyPUCuNFI1\|ryPLh?=	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTBzN{W2Nkc,OTlyMUe1OlI9N2F-
Hep3B	MX3GeY5kfGmxbjDhd5NigQ>?		MXOzNEBucW6\|	MWDJcohq[mm2aX;uJI9nKGi7cH;4bYEucW6mdXPl[EBJUUZzYXzwbIEheHKxdHXpckBi[2O3bYXsZZRqd25iaX6gbJVu[W5iSHXwN2Ih[2WubIOgeJJm[XSnZDDmc5IhOzBibXnud{Bu\WG\|dYLl[EBi\nSncjCxNkBpenNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{NCCLQ{WwQVAvODV5Mt88UU4>	M37pdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEeyNlE1Lz5zOUC3NlIyPDxxYU6=
Hep3B	MnjySpVv[3Srb36gZZN{[Xl?		NFP6cZcyPiCqcoO=	MoHmTY5pcWKrdHnvckBw\iCqeYDvfIliNWmwZIXj[YQhXkWJRjDwdo91\WmwIIPlZ5JmfGmxbjDpckBpfW2jbjDI[ZA{SiClZXzsd{Bi\nSncjCxOkBpenNiYomgSWxKW0FuIFnDOVA:OC5yN{m1{txONg>?	NGfGRYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUC3NlIyPCd-MUmwO\|IzOTR:L3G+
HCT116	NWnqVlZvS3m2b4TvfIlkcXS7IHHzd4F6		NEXkdmk4OiCqcoO=	MUfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgO\|IhcHK\|LDDJR\|UxRTBwMEW3{txONg>?	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTJ\|MUi2OEc,OTl{M{G4OlQ9N2F-
SKBR3	MlHUR5l1d3SxeHnjbZR6KGG\|c3H5		NIOwO2k4OiCqcoO=	NV3EUGVSS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0uEUkOgZ4VtdHNiYX\0[ZIhPzJiaILzMEBKSzVyPUCuNFU5\|ryPLh?=	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTJ\|MUi2OEc,OTl{M{G4OlQ9N2F-
MCF7	M171XmN6fG:2b4jpZ4l1gSCjc4PhfS=>		MoXJO\|IhcHK\|	NV23b5NGS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkA4OiCqcoOsJGlEPTB;MD6wO\|HPxE1w	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTJ\|MUi2OEc,OTl{M{G4OlQ9N2F-
SKOV3	M4O2XWN6fG:2b4jpZ4l1gSCjc4PhfS=>		MVu3NkBpenN?	MnPaR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2tQXjNiY3XscJMh[W[2ZYKgO\|IhcHK\|LDDJR\|UxRTBwMUKy{txONg>?	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTJ\|MUi2OEc,OTl{M{G4OlQ9N2F-
SKBR3	MnXwR5l1d3SxeHnjbZR6KGG\|c3H5		M2CzVVczKGi{cx?=	MVfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT2JTOyClZXzsd{Bi\nSncjC3NkBpenNiaX6gdJJme2WwY3Wgc4YhVlGRMTDpcohq[mm2b4Kg[Ilkd3WvYYLvcEwhUUN3ME2wMlI{\|ryPLh?=	NWPaSHMxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmyN\|E5PjRpPkG5NlMyQDZ2PD;hQi=>
MCF7	NYnqeZU1S3m2b4TvfIlkcXS7IHHzd4F6		NHTYNZc4OiCqcoO=	MVfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCrbjDwdoV{\W6lZTDv[kBPWU9zIHnubIljcXSxcjDkbYNwfW2jcn;sMEBKSzVyPUCuPFYz\|ryPLh?=	Mk\hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl{M{G4OlQoRjF7MkOxPFY1RC:jPh?=
NCI-H596	Mnv1R5l1d3SxeHnjbZR6KGG\|c3H5		NGLhVoQ4OiCqcoO=	M3SzXWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KE6TMEGt[IVncWOrZX70JIh2dWGwIF7DTU1JPTl4IHPlcIx{KGGodHXyJFczKGi{czygTWM2OD1zLkJOwG0v	NF\vTXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUKzNVg3PCd-MUmyN\|E5PjR:L3G+
MDA468	NX;wPG9VS3m2b4TvfIlkcXS7IHHzd4F6		M4O2OVczKGi{cx?=	NUjtZnFuS3m2b4TvfIlkcXS7IHHnZYlve3RiTmGwNU1l\W[rY3nlcpQhcHWvYX6gUWRCPDZ6IHPlcIx{KGGodHXyJFczKGi{czygTWM2OD1zLkdOwG0v	NXHOd\|ZXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmyN\|E5PjRpPkG5NlMyQDZ2PD;hQi=>
SKBR3	M1TQeWN6fG:2b4jpZ4l1gSCjc4PhfS=>		NY\O[Vl6PzJiaILz	NXr5bIZtS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0uEUkOgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGOnbHz0bZRmei2pbH:gZZN{[XluIFnDOVA:OC5yMkVOwG0v	M2DxSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NEC1OVI5Lz5zOUSwOVUzQDxxYU6=
A549	M3:zNWN6fG:2b4jpZ4l1gSCjc4PhfS=>		MnfhO\|IhcHK\|	MWXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDj[YxtfGm2ZYKt[4xwKGG\|c3H5MEBKSzVyPUCuNFY5\|ryPLh?=	MkPOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2MEW1NlgoRjF7NEC1OVI5RC:jPh?=
SKOV3	MXzDfZRwfG:6aXPpeJkh[XO\|YYm=		M2LFdlczKGi{cx?=	M4LHR2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNMV1Z\|IHPlcIx{KGGodHXyJFczKGi{czDifUBk\WyudHn0[ZIu\2yxIHHzd4F6NCCLQ{WwQVAvOjMQvF2u	NFPVfY09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUSwOVUzQCd-MUm0NFU2Ojh:L3G+
MCF7	NHK0OGtEgXSxdH;4bYNqfHliYYPzZZk>		MmnpO\|IhcHK\|	NXvUUm16S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkA4OiCqcoOgZpkh[2WubITpeIVzNWeubzDhd5NigSxiSVO1NF0xNjJ\|zszNMi=>	NITOUGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUSwOVUzQCd-MUm0NFU2Ojh:L3G+
CCRF-CEM	NGDwTYpEgXSxdH;4bYNqfHliYYPzZZk>		M1rq[FczKGi{cx?=	NVjufmdmS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hS0OURj3DSW0h[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IHPlcIx1cXSncj25OkBieXWnb4XzJI9v\SC\|b3z1eIlwdiCjc4PhfUwhUUN3ME2wMlU1\|ryPLh?=	NWTWPYdURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0NFU2OjhpPkG5OFA2PTJ6PD;hQi=>
CCRF-CEM	NWm3U5VLS3m2b4TvfIlkcXS7IHHzd4F6		NVjqb5JRPzJiaILz	M4DkPGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJJBi[2yrdHH4[YwuemW\|aYP0ZY51KEOFUl[tR2VOKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDj[YxtfGm2ZYKtPVYh[XG3ZX;1d{BwdmVic3;seZRqd25iYYPzZZktKEmFNUC9Nk42\|ryPLh?=	MlHSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2MEW1NlgoRjF7NEC1OVI5RC:jPh?=
Hep3B	MWXGeY5kfGmxbjDhd5NigQ>?		M37kfFMxKG2rboO=	MULJcohq[mm2aX;uJI9nKGi7cH;4bYEucW6mdXPl[EBJUUZzYXzwbIEheHKxdHXpckBi[2O3bYXsZZRqd25iaX6gbJVu[W5iSHXwN2Ih[2WubIOgeJJm[XSnZDDmc5IhOzBibXnud{Bu\WG\|dYLl[EBi\nSncjCxNkBpenNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{NCCLQ{WwQVAvODV5zszNMi=>	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR4OUi4O{c,OjB2Nkm4PFc9N2F-
Hep3B	NFi1botHfW6ldHnvckBie3OjeR?=		MkDJNVYhcHK\|	MVnJcohq[mm2aX;uJI9nKGi7cH;4bYEucW6mdXPl[EBXTUeIIIDyc5RmcW5ic3XjdoV1cW:wIHnuJIh2dWGwIFjldFNDKGOnbHzzJIFnfGW{IEG2JIhzeyCkeTDFUGlUSSxiSVO1NF0xNjB5Od88UU4>	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR4OUi4O{c,OjB2Nkm4PFc9N2F-
HCT116	NV\F[VR3S3m2b4TvfIlkcXS7IHHzd4F6			M{\wZWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDFzNjDj[YxteyCkeTDBcIFu[XJiYnz1[UBie3OjeTygTWM2OD1yLkC1{txONg>?	MnvOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB4NkK1N\|QoRjJyNk[yOVM1RC:jPh?=
NCI-H1299	MX3GeY5kfGmxbjDhd5NigQ>?		NXXjfXpUOjRiaILz	M3y2[GlvcGmkaYTpc44hd2ZiaIXtZY4hUFOSOUCgbY4hcHWvYX6gUmNKNUhzMkm5JINmdGy\|IHHzd4V{e2WmIHHzJGFsfCCmZXfyZYRifGmxbjDh[pRmeiB{NDDodpMh[nlibIXtbY5mgCCjc4PhfUwhUUN3ME2wMlHPxE1w	MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR\|OEW0NUc,OjF2M{i1OFE9N2F-
LN229-Lux	NWPnS5pbTnWwY4Tpc44h[XO\|YYm=	NVnoZpI2Oi53IITvJFExKHWP	M3roXVEhcHJ?	NHHkcHVKdmirYnn0bY9vKG:oIHz1Z4ln\XKjc3WgZYN1cX[rdImgbY4hcHWvYX6gUG4zOjlvTIX4JINmdGy\|IHH0JFIvPSC2bzCxNEB2VSCrbnP1ZoF1\WRiZn;yJFEhcHJidX7k[ZIhdm:{bX;4bYEh\m:ubH;3[YQh[nliMkSgbJJ{KHWwZHXyJIh6eG:6aXGgZpkhemWyb4L0[ZIh\2WwZTDhd5NigQ>?	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd2NkK3OEc,OjJ5NE[yO\|Q9N2F-
MCF7	NVz1eo5mSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NF70TZA1QCCqcoO=	M2TGfWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NE4{Qc7:TT6=	MoHQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3OEK0O\|coRjJ2NUiyOFc4RC:jPh?=
HCT116	M3zOe2FvfGmycn;sbYZmemG2aY\lJIF{e2G7		MVu0PEBpenN?	NHXEV\|ZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjDWFEyPiClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJINmdGxidnnhZoltcXS7IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OC55ON88UU4>	NHPNTGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEW4NlQ4Pyd-MkS1PFI1Pzd:L3G+
SKBR3	MYXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		MWG0PEBpenN?	NFHyOXhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPLRnI{KGOnbHzzJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yNjN2zszNMi=>	MlnrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3OEK0O\|coRjJ2NUiyOFc4RC:jPh?=
A231	MXHBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		MlXoOFghcHK\|	NIfrdYVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFGyN\|Eh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1yLkG3{txONg>?	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd4M{K2NUc,OjR5NkOyOlE9N2F-
MCF7	NH:zWJhCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NYTl[m1OPDhiaILz	NWLGemoySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MD64{txONg>?	NES4UHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe2N\|I3OSd-MkS3OlMzPjF:L3G+
HCT116	MX7BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NFTQOWw1QCCqcoO=	NI\hSGFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjDWFEyPiClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG\|c3H5MEBKSzVyPUGuNlHPxE1w	NFTzPWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe2N\|I3OSd-MkS3OlMzPjF:L3G+
SKBR3	M4\IcGFvfGmycn;sbYZmemG2aY\lJIF{e2G7		M17zbVQ5KGi{cx?=	NFO3bYdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPLRnI{KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;Mz6xNe69VS5?	M4rWNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{[zNlYyLz5{NEe2N\|I3OTxxYU6=
NCI-H1299	NU\jXWFRTnWwY4Tpc44h[XO\|YYm=		NX\Zd5pVOTJiaILz	MVzS[YR2[3Srb36gbY4hd3i7Z3XuJINwdnO3bYD0bY9vKHKjdHWgbY4hcHWvYX6gUmNKNUhzMkm5JINmdGy\|IHnuZ5Vj[XSnZDDmc5IhOTJiaILz	NHvrc\|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUO4N\|kyPSd-MkWzPFM6OTV:L3G+
PC9	Mn\JR5l1d3SxeHnjbZR6KGG\|c3H5		MlvmO\|IhcHK\|	NF3IWnpEgXSxdH;4bYNqfHliYXfhbY5{fCCKR1[tbY5lfWOnZDDldoxwfGmwaXKtdoV{cXO2YX70JIh2dWGwIGDDPUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHPlcIwh\3Kxd4ToJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MD6wNe69VS5?	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjh2NE[4PUc,OjZ6NES2PFk9N2F-
Ma1	NFSwRlREgXSxdH;4bYNqfHliYYPzZZk>		M2LCNlczKGi{cx?=	NFX3RXVEgXSxdH;4bYNqfHliYXfhbY5{fCCKR1[tbY5lfWOnZDDldoxwfGmwaXKtdoV{cXO2YX70JIh2dWGwIF3hNUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHPlcIwh\3Kxd4ToJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MD6wNe69VS5?	NUD3cm91RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[4OFQ3QDlpPkK2PFQ1Pjh7PD;hQi=>
SKBR3	MVzGeY5kfGmxbjDhd5NigQ>?			MluyTY5pcWKrdHnvckBw\iCKc4C5NEBqdiCqdX3hckBUU0KUMzDj[YxteyxiSVO1NF0xNjB{NN88UU4>	M2C3elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OES0Olg6Lz5{Nki0OFY5QTxxYU6=
HeLa	NEjP[JNHfW6ldHnvckBie3OjeR?=	NFHLb4kyOCC3TR?=	M3TjcVYhcHK\|	NH\qblhKdmirYnn0bY9vKG:oIFjTVFkxKGmwIHj1cYFvKEinTHGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5lfWO2aX;uJI9nKGOqa{Gg[IVoemGmYYTpc44h[XRiMUCgeW0h[W[2ZYKgOkBpenNiYomgW4V{fGW{bjDicI91KG2ndHjv[C=>	Mn[2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh6MU[0OFkoRjJ6OEG2OFQ6RC:jPh?=
HeLa	NX7mXGJPTnWwY4Tpc44h[XO\|YYm=	M4q5TlExKHWP	MmrOOkBpenN?	MorLTY5pcWKrdHnvckBw\iCKU2C5NEBqdiCqdX3hckBJ\UyjIHPlcIx{KGG\|c3Xzd4VlKGG\|IHnu[JVkfGmxbjDv[kBCc3RiZHXndoFl[XSrb36gZZQhOTBidV2gZYZ1\XJiNjDodpMh[nliV3XzeIVzdiCkbH;0JI1mfGixZB?=	M3jm[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OEG2OFQ6Lz5{OEixOlQ1QTxxYU6=
HeLa	MonxSpVv[3Srb36gZZN{[Xl?	MVexNEB2VQ>?	M2fGV\|YhcHK\|	MnL1TY5pcWKrdHnvckBw\iCKU2C5NEBqdiCqdX3hckBJ\UyjIHPlcIx{KGG\|c3Xzd4VlKGG\|IHnu[JVkfGmxbjDv[kBJW1B5MDDwdo91\WmwIHX4dJJme3Orb36gZZQhOTBidV2gZYZ1\XJiNjDodpMh[nliV3XzeIVzdiCkbH;0JI1mfGixZB?=	NGDUOWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEixOlQ1QSd-Mki4NVY1PDl:L3G+
PC3	NHn2SnJHfW6ldHnvckBie3OjeR?=	MXuxNEB2VQ>?	MkTPOkBpenN?	NEjIe5dKdmirYnn0bY9vKG:oIFjTVFkxKGmwIHj1cYFvKFCFMzDj[YxteyCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4Yh[2itMTDk[Ydz[WSjdHnvckBifCBzMDD1UUBi\nSncjC2JIhzeyCkeTDX[ZN1\XKwIHLsc5QhdWW2aH;k	MofDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh6MU[0OFkoRjJ6OEG2OFQ6RC:jPh?=
PC3	M2PRUmZ2dmO2aX;uJIF{e2G7	MljwNVAhfU1?	M3vrN\|YhcHK\|	NUSxe2RXUW6qaXLpeIlwdiCxZjDIV3A6OCCrbjDoeY1idiCSQ{OgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5lfWO2aX;uJI9nKEGtdDDk[Ydz[WSjdHnvckBifCBzMDD1UUBi\nSncjC2JIhzeyCkeTDX[ZN1\XKwIHLsc5QhdWW2aH;k	Ml7LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh6MU[0OFkoRjJ6OEG2OFQ6RC:jPh?=
PC3	M1vB[GZ2dmO2aX;uJIF{e2G7	M4HNflExKHWP	NEPDbnE3KGi{cx?=	M4PTd2lvcGmkaYTpc44hd2ZiSGPQPVAhcW5iaIXtZY4hWEN\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnu[JVkfGmxbjDv[kBJW1B5MDDwdo91\WmwIHX4dJJme3Orb36gZZQhOTBidV2gZYZ1\XJiNjDodpMh[nliV3XzeIVzdiCkbH;0JI1mfGixZB?=	MoTaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh6MU[0OFkoRjJ6OEG2OFQ6RC:jPh?=

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	HSP90 / HSP70 ; PubMed: 28915605 Oncotarget Western blot analysis showing dose-dependent elevation of Hsp70 and HSP90 levels in AGS cells by 17-DMAG. p-Akt / Survivin / MMP2 ; PubMed: 28915605 Oncotarget Western blot analysis showing the dose-dependent reduction of the expression of HSP90 client proteins, such as p-Akt, survivin, and MMP2 by 17-DMAG. PARP / Cleaved caspase-3 / Cleaved caspase-8 / Cleaved caspase-9 / PUMA ; PubMed: 28915605 Oncotarget (C-D) 17-DMAG effects on the expression of apoptotic proteins (PARP, c-caspase 3, c-caspase-8, c-caspase-9, and PUMA) in AGS cells. Western blot analyses indicate that 17-DMAG significantly increased the expression of apoptotic proteins in AGS cells in a dose- (C) and time- (D) dependent manner (P < 0.05). p-ALK / ALK / p-Akt / Akt / p-ERK / ERK ; PubMed: 26219569 BMC Cancer Modulation of ALK signaling in parental and 17-DMAG-resistant cells. Cells were treated with the indicated concentrations of 17-DMAG or AUY922 for 6 h. The molecules of ALK-related signaling activity were detected by western blot analysis. α-Tax / α-IKKα / α-IKKβ/ α-NEMO / α-TBK1 / α-p65 / α-p50 ; PubMed: 24109220 J Virol C8166, MT-2, and MT-4 cells were treated with 17-DMAG as indicated, and cell lysates were subjected to immunoblotting with the indicated antibodies.	28915605 26219569 24109220
Growth inhibition assay	Cell proliferation ; PubMed: 28915605 Oncotarget Proliferation assay of AGS cells treated with graded concentrations of 17-DMAG for 24 h or 48 h. 17-DMAG resulted in significant dose- and time-dependent reduction of AGS cell proliferation (P < 0.05)	28915605

In vivo

17-DMAG treatment at 5 mg/kg or 25 mg/kg thrice per week significantly reduces tumor growth of TMK-1 xenografts, by significantly reducing vessel area and numbers of proliferating tumor cells in sections. ^[2] Consistent the inhibition of FAK signaling in vivo, 17-DMAG treatment at 25 mg/kg three times a week significantly suppresses tumor growth, and metastasis of ME180 and SiHa xenografts in mice. ^[4] Administration of 17-DMAG at 10 mg/kg for 16 days significantly decreases the white blood cell count and prolongs the survival in a TCL1-SCID transplant mouse model. ^[5]

Protocol

Kinase Assay:^[1]	- Collapse Fluorescence polarization (FP)-based competition binding assay: This assay utilizes a boron difluoride dipyrromethene (BODIPY) labeled geldanamycin analogue (BODIPY-AG) as a probe and measured fluorescence polarization upon binding of the probe to a protein. Native human Hsp90 protein (α + β isoforms) is isolated from HeLa cells. BODIPY-AG solution is freshly prepared in FP assay buffer (20 mM HEPES-KOH, pH 7.3, 1.0 mM EDTA, 100 mM KCl, 5.0 mM MgCl₂, 0.01% NP-40, 0.1 mg/mL fresh bovine γ-globulin (BGG), 1.0 mM fresh DTT, and protease inhibitor from stock solution in DMSO. Competition curves are obtained by mixing 10 μL each of a solution containing BODIPY-AG and Hsp90, and a serial dilution of 17-DMAG freshly prepared in FP assay buffer from stock solution in DMSO. Final concentrations are 10 nM BODIPY-AG, 40 or 60 nM Hsp90, varying concentration of 17-DMAG (0.10 nM-10 μM), and ≤0.25% DMSO in a 384-well microplate. After 3 hours incubation at 30 °C, fluorescence anisotropy (γ_Ex = 485 nm, γ_Em = 535 nm) is measured on an EnVision 2100 multilabel plate reader. IC50 value of 17-DMAG is obtained from the competition curves.
Cell Research:^[5]	- Collapse Cell lines: Chronic lymphocytic leukemia (CLL) Concentrations: Dissolved in DMSO, final concentrations ~1 μM Incubation Time: 24, or 48 hours Method: Cells are exposed to various concentrations of 17-DMAG for 24, or 48 hours. For the assessment of cytotoxicity, MTT reagent is then added, and plates are incubated for an additional 24 hours before spectrophotometric measurement. Apoptosis is determined by staining with annexin V-fluorescein isothiocyanate and propidium iodide (PI). (Only for Reference)
Animal Research:^[5]	- Collapse Animal Models: SCID mice engrafted with TCL1 leukemia cells Dosages: 10 mg/kg Administration: Intraperitoneal injection 5 times per week (Only for Reference)

References

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Solubility (25°C)

In vitro	DMSO	131 mg/mL (200.54 mM)
	Water	Insoluble
	Ethanol	Insoluble
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 1% DMSO+30% polyethylene glycol+1% Tween 80 For best results, use promptly after mixing.	30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Alvespimycin (17-DMAG) HCl SDF

Molecular Weight	653.21
Formula	C₃₂H₄₈N₄O₈•HCl
CAS No.	467214-21-7
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	NSC 707545,BMS 826476 HCl,KOS 1022
Smiles	CC1CC(C(C(C=C(C(C(C=CC=C(C(=O)NC2=CC(=O)C(=C(C1)C2=O)NCCN(C)C)C)OC)OC(=O)N)C)C)O)OC.Cl

In vivo Formulation Calculator (Clear solution)

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Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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Molarity Calculator

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The Serial Dilution Calculator Equation

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Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
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Molecular Weight Calculator

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Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2021-09-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT00780000	Terminated	Drug: Alvespimycin	Breast Cancer	Bristol-Myers Squibb	April 2008	Phase 2
NCT00248521	Unknown status	Drug: alvespimycin hydrochloride	Unspecified Adult Solid Tumor Protocol Specific	Institute of Cancer Research United Kingdom\|National Cancer Institute (NCI)	October 2005	Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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HSP (HSP90) Signaling Pathway Map

HSP (HSP90) Inhibitors with Unique Features

Selective HSP90 Inhibitor

NVP-BEP800 : HSP90β-selective, IC50=58 nM.
Most Potent HSP90 Inhibitor

KW-2478 : HSP90, IC50=3.8 nM.
HSP90 Inhibitor in Clinical Trial

17-AAG (Tanespimycin) : Phase III for Gastrointestinal Stromal Tumors.
Newest HSP90 Inhibitor

XL888 : ATP-competitive inhibitor of HSP90 with IC50 of 24 nM.

Related HSP (HSP90) Products

Zelavespib (PU-H71) ^New

Zelavespib (PU-H71, NSC 750424) is a potent and selective inhibitor of HSP90 with IC50 of 51 nM. Phase 1.
KNK437 ^New

KNK437 is a pan-HSP inhibitor, which inhibits the synthesis of inducible HSPs, including HSP105, HSP72, and HSP40.
VER155008 ^New

VER155008 (C07) is a potent Hsp70 family inhibitor with IC50 of 0.5 μM, 2.6 μM, and 2.6 μM in cell-free assays for HSP70, HSC70, and GRP78 (HSPA5, Bip), respectively, >100-fold selectivity over HSP90. VER155008 inhibits autophagy and causes reduced levels of HSP90 client proteins.
Tanespimycin (17-AAG)

Tanespimycin (17-AAG, CP127374, NSC-330507, KOS 953) is a potent HSP90 inhibitor with IC50 of 5 nM in a cell-free assay, having a 100-fold higher binding affinity for HSP90 derived from tumour cells than HSP90 from normal cells. Tanespimycin (17-AAG) induces apoptosis, necrosis, autophagy and mitophagy. Phase 3.

Features:Displays very low toxicity toward normal cells.
Luminespib (NVP-AUY922)

Luminespib (AUY-922, NVP-AUY922, VER-52296) is a highly potent HSP90 inhibitor for HSP90α/β with IC50 of 13 nM /21 nM in cell-free assays, weaker potency against the HSP90 family members GRP94 and TRAP-1, exhibits the tightest binding of any small-molecule HSP90 ligand. Luminespib (AUY-922, NVP-AUY922) effectively downregulates and destabilizes the IGF-1Rβ protein and results in growth inhibition, autophagy and apoptosis. Phase 2.
Ganetespib (STA-9090)

Ganetespib (STA-9090) is an HSP90 inhibitor with IC50 of 4 nM in OSA 8 cells, induces apoptosis of OSA cells while normal osteoblasts are not affected; active metabolite of STA-1474. Phase 3.
Geldanamycin (NSC 122750)

Geldanamycin (NSC 122750) is a natural existing HSP90 inhibitor with K_d of 1.2 μM, specifically disrupts glucocorticoid receptor (GR)/HSP association. Geldanamycin attenuates virus infection-induced ALI (acute lung injury)/ARDS (acute respiratory distress syndrome) by reducing the host's inflammatory responses.
HSP990 (NVP-HSP990)

HSP990 (NVP-HSP990) is a novel, potent and selective HSP90 inhibitor for HSP90α/β with IC50 of 0.6 nM/0.8 nM. NVP-HSP990 induces cell cycle arrest and apoptosis.

Features:NVP-HSP990 is an orally available HSP90 inhibitor and is structurally distinct from other clinical HSP90 inhibitors.
Elesclomol (STA-4783)

Elesclomol (STA-4783) is a novel potent oxidative stress inducer that elicits pro-apoptosis events among tumor cells. Phase 3.
Onalespib (AT13387)

Onalespib (AT13387) is a selective potent Hsp90 inhibitor with IC50 of 18 nM in A375 cells, displays a long duration of anti-tumor activity. Phase 2.

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Alvespimycin (17-DMAG) HCl