Elesclomol (STA-4783)

Catalog No.S1052

For research use only.

Elesclomol (STA-4783) is a novel potent oxidative stress inducer that elicits pro-apoptosis events among tumor cells. Phase 3.

Elesclomol (STA-4783) Chemical Structure

CAS No. 488832-69-5

Selleck's Elesclomol (STA-4783) has been cited by 12 Publications

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Biological Activity

Description Elesclomol (STA-4783) is a novel potent oxidative stress inducer that elicits pro-apoptosis events among tumor cells. Phase 3.
Targets
HSP70 [1]
(Cell-free assay)
In vitro

Elesclomol significantly induces the expression of heat shock stress response genes and metallothionein genes, a signature transcription profile indicative of oxidative stress in Hs294T cells. Elesclomol (100 nM) rapidly induces Hsp70 RNA levels with a 4.8-fold increase at 1 hour and a 160-fold increase at 6 hours in Ramos Burkitt's lymphoma B cells in consistent with the intracellular ROS content which increases by 20% as early as 0.5 hour and 385% at 6 hours, and the induction of Hsp70 can be blocked by antioxidants N-acetylcysteine (NAC) and Tiron pretreatment. Elesclomol increases the number of early and late apoptotic cells with 3.7- and 11-fold through the induction of oxidative stress, which can be completely blocked by NAC, while having little effect on normal cells. [1] Elesclomol significantly inhibits the cell viability of SK-MEL-5, MCF-7, and HL-60 with IC50 of 110 nM, 24 nM and 9 nM, respectively. [2] Elesclomol induces copper-dependent ROS generation and cytoxicity in yeast. Instead of working through a specific cellular protein target, Elesclomol interacts with the electron transport chain (ETC), a biologically coherent set of processes occurring in the mitochondrion, to generate high levels of ROS within the organelle and consequently cell death. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
PC3 cells MVfGeY5kfGmxbjDhd5NigQ>? MXLJcohq[mm2aX;uJI9nKFCGS{GtcYVlcWG2ZXSgRWtVKHCqb4PwbI9zgWyjdHnvckBifCCWaIKzNFghemW|aXT1[UBqdiCqdX3hckBRSzNiY3XscJMh[nliRVzJV2EtKEmFNUC9NE4yOTNizszN NU[zPXppRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzOFE3PzVpPkKxN|QyPjd3PD;hQi=>
F-36P cells MmXSVJJwdGmoZYLheIlwdiCjc4PhfS=> NIrhd3VCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF[tN|ZRKGOnbHzzMEBKSzVyPUCuNlgh|ryP NITZd4M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUO0NVY4PSd-MkGzOFE3PzV:L3G+
OCI-AML2 cells MV7Qdo9tcW[ncnH0bY9vKGG|c3H5 M2rQfGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iT1PJMWFOVDJiY3XscJMtKEmFNUC9NE4{PSEQvF2= M2e3NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{SxOlc2Lz5{MUO0NVY4PTxxYU6=
K562 cells NF7IUm1Rem:uaX\ldoF1cW:wIHHzd4F6 MYPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEt3NkKgZ4VtdHNuIFnDOVA:OThizszN MmXTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|NEG2O|UoRjJzM{SxOlc2RC:jPh?=
MES-SA NIK3UpJEgXSxdH;4bYNqfHliYYPzZZk> NXK4OpBWS3m2b4TvfIlkcXS7IHHnZYlve3RibYXseIllenWpLYLld4l{fGGwdDDoeY1idiCPRWOtV2Eh[2WubIOsJGlEPTB;MD6wNFXPxE1w M323d|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUO3PVgyLz5{M{mzO|k5OTxxYU6=
MES-SA/Dx5 Mny2R5l1d3SxeHnjbZR6KGG|c3H5 MojIR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWVUNVODL1T4OUBk\WyuczygTWM2OD1yLkCwOe69VS5? M1n1T|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUO3PVgyLz5{M{mzO|k5OTxxYU6=
Bowes Mmr4R5l1d3SxeHnjbZR6KGG|c3H5 M2[0TmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG23bITp[JJ2\y2{ZYPpd5RidnRiaIXtZY4hSm:5ZYOgZ4VtdHNuIFnDOVA:OC5yMd88UU4> Mn;2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7M{e5PFEoRjJ|OUO3PVgyRC:jPh?=
DU145 Ml\4R5l1d3SxeHnjbZR6KGG|c3H5 NGHCR3pEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBFXTF2NTDj[YxteyxiSVO1NF0xNjB{zszNMi=> NH7SepU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mzO|k5OSd-MkO5N|c6QDF:L3G+
Ramos NEmxbnhHfW6ldHnvckBie3OjeR?= MUPJcoR2[3Srb36gc4YhUHOyN{CgdJJw\HWldHnvckBqdiCqdX3hckBT[W2xczDj[YxteyCjZoTldkA2KGi{czDifUBGVEmVQTygSWM2OD1yLkCy{txONg>? MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl|N{m4NUc,OjN7M{e5PFE9N2F-
HL60 NETj[21EgXSxdH;4bYNqfHliYYPzZZk> M4fKfmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG23bITp[JJ2\y2{ZYPpd5RidnRiaIXtZY4hUEx4MDDj[YxteyxiSVO1NF0xNjB3zszNMi=> MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl|N{m4NUc,OjN7M{e5PFE9N2F-
MES-SA NUL4UXBvS3m2b4TvfIlkcXS7IHHzd4F6 MmHtR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWVUNVODIHPlcIx{NCCLQ{WwQVAvODYQvF2u MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl|N{m4NUc,OjN7M{e5PFE9N2F-
MIP101 MmTQR5l1d3SxeHnjbZR6KGG|c3H5 MY\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNTXAyODFiY3XscJMtKEmFNUC9NE4xPc7:TT6= MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl|N{m4NUc,OjN7M{e5PFE9N2F-
A549 NHzLO3JEgXSxdH;4bYNqfHliYYPzZZk> MV\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzMEBKSzVyPUCuNe69VS5? NHHNfYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mzO|k5OSd-MkO5N|c6QDF:L3G+
Clone A Ml6yR5l1d3SxeHnjbZR6KGG|c3H5 MoLMR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gR4xwdmViQTDj[YxteyxiSVO1NF0xNjIQvF2u MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl|N{m4NUc,OjN7M{e5PFE9N2F-
Bowes M2HLdWN6fG:2b4jpZ4l1gSCjc4PhfS=> M3nNUGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KHerbHSgeJlx\SCqdX3hckBDd3enczDj[YxteyxiSVO1NF0xNjMQvF2u NFvPTHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mzO|k5OSd-MkO5N|c6QDF:L3G+
HL60 NX3CSWRNS3m2b4TvfIlkcXS7IHHzd4F6 MWfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzMEBKSzVyPUCuOO69VS5? NVLhVHVrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5N|c6QDFpPkKzPVM4QThzPD;hQi=>
MDA435 MWrDfZRwfG:6aXPpeJkh[XO|YYm= MWXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGE1OzViY3XscJMtKEmFNUC9Oe69VS5? NWrjbmc6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5N|c6QDFpPkKzPVM4QThzPD;hQi=>
MDA-MB-435 MofKRY51cXS3bX;yJIF{e2G7 NE\QdpAzPSCvZz;r[y=> M3q4R2FvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi12M{WgZ4VtdHNieHXuc4dz[W[2ZXSgbY4hS0RvMTDueU9vfSCvb4Xz[UBie3Onc4Pl[EBieyC2dX3vdkBz\We{ZYPzbY9vKGG2IEK1JI1oN2upLDDpekByd2RiYXTtbY5qe3SncnXkJFMhfGmvZYOgZUB4\WWtIH\vdkAzKGOxboPlZ5V1cX[nIIfl[Yt{ M1jObFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUO3PVgyLz5{M{mzO|k5OTxxYU6=
NOS-1 NGLsfm1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1W4T2lvcGmkaYTpc44hd2ZiaIXtZY4hVk:VLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlAxODB|NkdOwG0v NVz3NnQ6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTl5Mki2NE8oRlODTlfFVlww[T5?
CAL-51 NFLHNXpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnP1TY5pcWKrdHnvckBw\iCqdX3hckBESUxvNUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlAxODF{NkVOwG0v M3jMe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE6PzJ6NkCvK|5USU6JRWK8M4E,
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HUTU-80 MlnJS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWHlN|RmUW6qaXLpeIlwdiCxZjDoeY1idiCKVWTVMVgxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5yMEC3PVEy|ryPLh?= NWX0blVERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTl5Mki2NE8oRlODTlfFVlww[T5?
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In vivo Although Elesclomol (25-100 mg/kg) as a single agent shows no antitumor activity in nude mouse xenograft models of human breast cancers (MDA435, MCF7 and ZR-75-1), lung cancer (RER) or lymphoma (U937), Elesclomol substantially enhances the efficacy of chemotherapeutic agents such as paclitaxel in these models, both in terms of tumor regression and extended survival of mice. [4]

Protocol (from reference)

Cell Research:[1]
  • Cell lines: Hs294T, HSB2, and Ramos
  • Concentrations: Dissolved in DMSO at a concentration of 10 mM, final concentrations ~500 nM
  • Incubation Time: 18, or 24 hours
  • Method: Cells are treated with various concentrations of Elesclomol for 18 or 24 hours. The level of intracellular ROS is monitored using the DCFDA probe, which emits a green fluorescence on oxidation. Cell death is determined by flow cytometry of cells double stained with Annexin V/FITC and propidium iodide (PI) using a Vybrant Apoptosis assay kit.
  • (Only for Reference)
Animal Research:[4]
  • Animal Models: Female CD-1 nude mice bearing established MDA435 breast cancer xenograft tumors
  • Dosages: ~100 mg/kg
  • Administration: Intravenous injection
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 80 mg/mL
(199.75 mM)
Water Insoluble
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
5% DMSO+40%PEG300+5%Tween80 +50%H2O
For best results, use promptly after mixing.

10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 400.5
Formula

C19H20N4O2S2

CAS No. 488832-69-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CN(C(=S)C1=CC=CC=C1)NC(=O)CC(=O)NN(C)C(=S)C2=CC=CC=C2

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT00827203 Suspended Drug: Elesclomol Sodium Metastatic Solid Tumors Synta Pharmaceuticals Corp. January 2009 Phase 1
NCT00808418 Completed Drug: Elesclomol Sodium|Drug: Docetaxel Prostate Cancer Synta Pharmaceuticals Corp. November 2008 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
How can we use the compound for in vivo mouse studies?

Answer:
S1052 in 1% DMSO+30% polyethylene glycol+1% Tween 80 is a suspension, it is fine for oral administration. You can also dissolve S1052 in 0.5-1% CMC-Na solution and treat the mice by oral gavage.

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