Niclosamide

Name: Niclosamide
Brand: Selleck Chemicals
SKU: S3030
Rating: 5 (26 reviews)

For research use only.

Catalog No.S3030 Synonyms: Niclocide, BAY2353

26 publications

CAS No. 50-65-7

Niclosamide (Niclocide, BAY2353) can inhibit DNA replication and inhibit STAT3 with IC50 of 0.7 μM in a cell-free assay. Niclosamide selectively inhibited the phosphorylation of STAT3 and had no obvious inhibition against the activation of other homologues (e.g., STAT1 and STAT5).

Size

Price

Stock

Quantity

50mg	USD 97	In stock
Bulk Size	Bulk Discount

Bulk Inquiry

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck USA Tel: (832) 582-8158 [email protected]

Selleck's Niclosamide has been cited by 26 publications

Cell Death Dis, 2020, 11(12):1035

PubMed: 33279931 ( click the link to review the publication )

Cell Death Dis, 2020, 11(6):466

PubMed: 32546700 ( click the link to review the publication )

J Cell Mol Med, 2020, 5

PubMed: 32372557 ( click the link to review the publication )

Biomed J, 2020, S2319-4170(20)30066-4

PubMed: 32563698 ( click the link to review the publication )

Clin Exp Pharmacol Physiol, 2020, 4

PubMed: 32248559 ( click the link to review the publication )

bioRxiv, 2020, 2020/9/20.4.7.30734

PubMed: 32511305 ( click the link to review the publication )

J Invest Dermatol, 2020, S0022-202X(20)31981-3

PubMed: 32805217 ( click the link to review the publication )

Nat Microbiol, 2019, 4(5):813-825

PubMed: 30833724 ( click the link to review the publication )

J Immunother Cancer, 2019, 7(1):245

PubMed: 31511071 ( click the link to review the publication )

Mol Immunol, 2019, 114:369-377

PubMed: 31450182 ( click the link to review the publication )

Fish Shellfish Immunol, 2019, 92:813-820

PubMed: 31271840 ( click the link to review the publication )

Oncol Rep, 2019, 41(4):2389-2395

PubMed: 30816524 ( click the link to review the publication )

Thorac Cancer, 2019, 10(4):848-855

PubMed: 30810286 ( click the link to review the publication )

Eur Rev Med Pharmacol Sci, 2019, 23(3):1055-1062

PubMed: 30779072 ( click the link to review the publication )

Exp Ther Med, 2019, 18(5):4022-4030

PubMed: 31611940 ( click the link to review the publication )

J Clin Endocrinol Metab, 2018, 103(10):3706-3713

PubMed: 30053001 ( click the link to review the publication )

Oncol Rep, 2018, 40(6):3743-3751

PubMed: 30272302 ( click the link to review the publication )

Autoimmunity, 2018, 51(4):191-198

PubMed: 29869537 ( click the link to review the publication )

Acta Pharm Sin B, 2018, 8(6):889-899

PubMed: 30505658 ( click the link to review the publication )

Neoplasia, 2018, 20(10):1008-1022

PubMed: 30189359 ( click the link to review the publication )

Oncol Lett, 2018, 15(4):5772-5780

PubMed: 29545902 ( click the link to review the publication )

Oncotarget, 2018, 9(10):9273-9284

PubMed: 29507689 ( click the link to review the publication )

Onco Targets Ther, 2017, 10:1767-1776

PubMed: 28367059 ( click the link to review the publication )

Oncotarget, 2017, 8(33):54265-54276

PubMed: 28903339 ( click the link to review the publication )
Int Immunopharmacol, 2016, 36:199-204

PubMed: 27160867 ( click the link to review the publication )

Neoplasia, 2015, 17(7):586-97

PubMed: 26297436 ( click the link to review the publication )

Purity & Quality Control

Choose Selective STAT Inhibitors

Biological Activity

Description

Targets

STAT3 ^[1]
(in Hela cells)

0.7 μM

In vitro

Niclosamide (< 5 μM) dose dependently inhibits STAT3-mediated luciferase reporter activity with IC50 of 0.25 μM in HeLa cells. Niclosamide(< 2 μM) dose dependently inhibits the phosphorylation of STAT3 in Du145 cells. Niclosamide (1 μM) inhibits the EGF-induced nuclear translocation of STAT3 in Du145 cells. Niclosamide(< 2 μM) dose dependently inhibits the transcription of STAT3 downstream genes in Du145 cells. Niclosamide(< 10 μM) dose dependently induces G0/G1 arrest and apoptosis of Du145 cancer cells. ^[1] Niclosamide is able to inhibit SARS-CoV replication at a micromolar concentration in Vero E6 cells infected with SARS-CoV. ^[2] Niclosamide(< 7.5 μM) promotes Frizzled1 endocytosis, downregulates Dishevelled-2 protein, and inhibits Wnt3A-stimulated beta-catenin stabilization and LEF/TCF reporter activity in U2OS cells. ^[3] Niclosamide inhibits the TNF-induced NF-κB reporter activity in a dose- and time-dependent manner in U2OS cells. Niclosamide(125 nM) inhibits NF-κB activation induced by p65, IKKα, IKKβ, IKKγ, and TAK1 in U2OS cells. Niclosamide(< 500 nM) completely block the time- and dose-dependent TNFα-induced alteration of the NF-κB–DNA complex in HL-60 cells. Niclosamide(< 10 nM) inhibits constitutive NF-κB activation in U266 cells. Niclosamide inhibits TNF-induced degradation of IκBα and relocation of p65 in a dose- and time-dependent manner in HL-60, Molm13, or AML primary cells. Niclosamide(500 nM) decreases TNF-induced NF-κB–dependent gene products involved in cell survival in HL-60 cells. Niclosamide dose dependently inhibits the growth and induces robust apoptosis of AML cells associated with decreased Mcl-1 and XIAP levels and increased intracellular ROS levels. ^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
PC3	NX3zWJV1SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NWD6ZnJGOTJyIHjydy=>	NXfpcJo4SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDQR\|Mh[2WubIOgZYZ1\XJiMUKwJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MD60{txO	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjZ6MEG1PUc,OTZ4OECxOVk9N2F-
A549	NUHaRYN7SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NIDHSW8yOjBiaILz	Mn3DRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCDNUS5JINmdGy\|IHHmeIVzKDF{MDDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRTBwNN88US=>	NYf6TXA{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[2PFAyPTlpPkG2OlgxOTV7PD;hQi=>
U87MG	MoHhRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NYLxOHdXOTJyIHjydy=>	MnjuRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCXOEfNS{Bk\WyuczDh[pRmeiBzMkCgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1yLkVOwG0>	MnTZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
LoVo	NI\iUlJCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		MWmxNlAhcHK\|	M1zKOWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTH;Wc{Bk\WyuczDh[pRmeiBzMkCgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1yLkhOwG0>	NHGwc2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNk[4NFE2QSd-MU[2PFAyPTl:L3G+
MIAPaCa2	Mo[2RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NFWxUVUyOjBiaILz	NHHUbpdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3JRXBiS2F{Y3XscJMh[W[2ZYKgNVIxKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NU4y\|ryP	NHn4VlM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNk[4NFE2QSd-MU[2PFAyPTl:L3G+
PC3	NEO1eJpHfW6ldHnvckBie3OjeR?=		NEnkdIQyKGi{	NHPnRVlKdmirYnn0bY9vKG:oIH3peI9kcG:wZILpZYwhdWWvYoLhcoUheG:2ZX70bYFtKGmwIHj1cYFvKFCFMzDj[YxteyCjZoTldkAyKGi{	NYO3e3l{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[2PFAyPTlpPkG2OlgxOTV7PD;hQi=>
HEK293	MXvGeY5kfGmxbjDhd5NigQ>?		NIHMV2syKGi{	NYTVPWZpUW6qaXLpeIlwdiCxZjDtbZRw[2ixbnTybYFtKG2nbXLyZY5mKHCxdHXueIlidCCrbjDoeY1idiCKRVuyPVMh[2WubIOgZYZ1\XJiMTDodi=>	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjZ6MEG1PUc,OTZ4OECxOVk9N2F-
neural precursor	M3\pPWZ2dmO2aX;uJIF{e2G7			NX;lXZJqUW6qaXLpeIlwdiCxZjDu[ZVzd3OyaHXy[UBxem:uaX\ldoF1cW:wIH;mJI1wfXOnIH7leZJidCCycnXjeZJ{d3JiY3XscJMh[nliTWTUJIF{e2G7	NGC5XoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{SxO\|Y{OSd-MUe0NVc3OzF:L3G+
Vero E6	NE\EO5hCdnSrdnnyZYwh[XO\|YYm=			MoHMRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgV2FTWyClb4LvcoF3cXK3czDpckBX\XKxIFW2JINmdGy\|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyZYwhemWybHnjZZRqd25iYomgSWxKW0FuIFXDOVA9OC5zzszN	M2TOR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5Nk[zOVM6Lz5zN{[2N\|U{QTxxYU6=
Vero E6	MWPDfZRwfG:6aXPpeJkh[XO\|YYm=			M2TyWGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KF[ncn:gSVYh[2WubIOgZpkhVVSWIHHzd4F6NCCFQ{WwQVIzNjIQvF2=	MnPuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4NkO1N\|koRjF5Nk[zOVM6RC:jPh?=
RAW264.7	MVfDfZRweHKxdHXjeIl3\SCjc4PhfS=>	MmroNVAhfU1?		MoDQR5l1d3C{b4TlZ5RqfmViYXP0bZZqfHliYXfhbY5{fCCjboTodoF5KHSxeHnuJIxmfGijbDDmZYN1d3JxcILveIVkfGm4ZTDhcpRq\2WwLXnu[JVk\WRiY3XscEBl\WG2aDDpckBud3W\|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjdDCxNEB2VSCkeTDNWHQhemWmdXP0bY9vKGG\|c3H5	M3H6[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NUSwO\|Y1Lz5zOUW0NFc3PDxxYU6=
CHO	M3fnfWN6fG:ycn;0[YN1cX[nIHHzd4F6			NInKbW9EgXSxcILveIVkfGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHHueIhz[XhiZoXzbY9vKHSxeHnuJGZRPTlvaX7keYNm\CClZXzsJIRm[XSqIHnuJGNJVyClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBjgSCPVGSgdoVlfWO2aX;uJIF{e2G7	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTV2MEe2OEc,OTl3NEC3OlQ9N2F-
CHO	MmPISpVv[3Srb36gZZN{[Xl?			M2ThSmlvcGmkaYTpc44hd2ZiQnHjbYxtfXNiYX70bJJi[2m\|IHHueIhz[XhicILveIVkfGm4ZTDhcpRq\2WwIHjldJRidWW{IIDy[U1xd3KnIITvJJBwemViY3;ueoVze2mxbjDpckBEVUd{LXX4dJJme3OrbnegR2hQKGOnbHzz	NV36dJV7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1OFA4PjRpPkG5OVQxPzZ2PD;hQi=>
Ava5	MWnBcpRqfmm{YXygZZN{[Xl?		M{e2ZlMh\GG7cx?=	MV\BcpRqfmm{YXygZYN1cX[rdImgZYdicW6\|dDDIR3Yh\2Wwb4T5dIUhOWJiaX7m[YN1\WRiaX6gRZZiPSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqemGuIILldIxq[2G2aX;uJIFnfGW{IEOg[IF6eyCkeTDicI91KGi7YoLp[Il7[XSrb36gZY5idHm\|aYOsJGVEPTB;MD6xOu69VQ>?	MljNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyNUm5PFMoRjJ{MEW5PVg{RC:jPh?=
Ava5	M{PmVGFvfGm4aYLhcEBie3OjeR?=		MXyzJIRigXN?	MkPyRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGNXKGenbn;0fZBmKDGkIHnu[oVkfGWmIHnuJGF3[TViY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB3cXKjbDDy[ZBtcWOjdHnvckBi\nSncjCzJIRigXNiYomgZoxwfCCqeXLybYRqgmG2aX;uJIFv[Wy7c3nzMEBGSzlyPUCuO:69VQ>?	NVjMT3BZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwOVk6QDNpPkKyNFU6QTh\|PD;hQi=>
Ava5	M{PWdmN6fG:2b4jpZ4l1gSCjc4PhfS=>		Ml;PN{Bl[Xm\|	MWfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBeoE2KGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDu[ZV1emGuIILl[EBlgWViYYPzZZktKEOFNUC9NVDPxE1?	M4C3XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEW5PVg{Lz5{MkC1PVk5OzxxYU6=
HFF	M3XpPGFvfGmjcHnjc41xdGW6YX6gZZN{[Xl?		NInYS3MzPCCqcoO=	M3HTemFvfGmjcHnjc41xdGW6YX6gZYN1cX[rdImgZYdicW6\|dDDUc5hweGyjc33hJIdwdmSraTDSTEB1[WOqeYrvbZRmeyCrbn\lZ5Rm\CCrbjDISmYh[2WubIOgZZN{\XO\|ZXSgZZMhYzOKXT31doFkcWxiaX7jc5Jxd3KjdHnvckBi\nSncjCyOEBpenNiYomgd4NqdnSrbHzheIlwdiCudX3pcoV{[2WwY3WgZ492dnSncjygUWlEQTB;MD6y{txO	Mo[yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7N{C5N\|coRjJ{OUewPVM4RC:jPh?=
HFF	MmfsRY51cWGyaXPvcZBt\XijbjDhd5NigQ>?		M2rmUlI1KGi{cx?=	NIDGTGVCdnSrYYDpZ49ueGyneHHuJIFkfGm4aYT5JIFo[Wmwc4SgWI95d3CuYYPtZUBod26maXmgVmghfGGlaIn6c4l1\XNiaX7m[YN1\WRiaX6gTGZHKGOnbHzzJIF{e2W\|c3XkJIF{KFt\|SG2teZJi[2muIHnuZ49zeG:{YYTpc44h[W[2ZYKgNlQhcHK\|IHL5JJNkcW62aXzsZZRqd25ibIXtbY5me2OnbnPlJINwfW62ZYKsJG1KSzVyPUCuNu69VQ>?	NVeyNHltRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5O\|A6OzdpPkKyPVcxQTN5PD;hQi=>
MDA-MB-231	NHn3[HpEgXSxdH;4bYNqfHliYYPzZZk>			M3K5dGN[fG:2b4jpZ4l1gSCjZ3HpcpN1KEWULX7l[4F1cX[nIHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[nliTWTTJIF{e2G7LDDJR\|UxRTBwN{pOwG0>	M{G3[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEG2NVkyLz5{M{SxOlE6OTxxYU6=
MCF7	NUL0S\|l3S3m2b4TvfIlkcXS7IHHzd4F6			MXfDXZRwfG:6aXPpeJkh[WejaX7zeEBGWi2yb4PpeIl3\SCqdX3hckBOS0Z5IHPlcIx{KGK7IF3UV{Bie3OjeTygTWM2OD1zLkC2{txO	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzRzNkG5NUc,OjN2MU[xPVE9N2F-
AsPC1	M{X4bmN6fG:2b4jpZ4l1gSCjc4PhfS=>			NUTaOYRIS1m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSXOSQ{GgZ4VtdHNiYomgUXRUKGG\|c3H5MEBKSzVyPUGuOFfPxE1?	MkPaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2MU[xPVEoRjJ\|NEG2NVkyRC:jPh?=
PANC1	NHnyfWhEgXSxdH;4bYNqfHliYYPzZZk>			MYHDXZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDQRW5EOSClZXzsd{BjgSCPVGOgZZN{[XluIFnDOVA:OS55M988US=>	NFj6[oo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SxOlE6OSd-MkO0NVYyQTF:L3G+
HEK293	MkDZSpVv[3Srb36gZZN{[Xl?		NX3EVY5VQCCqcoO=	MXHJcohq[mm2aX;uJI9nKFewdEPBM4JmfGFvY3Hz[YlvKHOrZ37hcIlv\yCrbjDISWszQTNiY3XscJMh[W[2ZYKgPEBpenNiYomgWG9R\myjc3igdoVxd3K2ZYKgZZN{[XluIFnDOVA:OC52zszN	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR3M{C3N{c,OjN2NUOwO\|M9N2F-
U2OS	M37RSWZ2dmO2aX;uJIF{e2G7	M2jkcFEzNjVidV2=	MWe2JIhzew>?	NXXlWWM2UW6mdXP0bY9vKG:oIHnueIVzdmGuaYrheIlwdiCxZjDGdol7gmynZEGtS2ZRKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIHj1cYFvKFV{T2OgZ4VtdHNiYYSgNVIvPSC3TTDh[pRmeiB4IHjyd{BjgSClb37mc4NidCCvaXPyc5Nkd3C7	NGrlV3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S1N\|A4Oyd-MkO0OVMxPzN:L3G+
MDA-MB-231	Mn36R5l1d3SxeHnjbZR6KGG\|c3H5		NHHLcoQ4OiCqcoO=	NHLVR5FEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBGWiCwZXfheIl3\SCPRFGtUWIuOjNzIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFNiYYPzZZktKEmFNUC9NE44Qc7:TR?=	NYfwVY5pRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVk3OTNpPkKzOFU6PjF\|PD;hQi=>
MCF7	NETlSZpEgXSxdH;4bYNqfHliYYPzZZk>		MW[3NkBpenN?	MXnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDFVkBxd3OrdHn2[UBOS0Z5IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFNiYYPzZZktKEmFNUC9NU4xPs7:TR?=	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR3OU[xN{c,OjN2NUm2NVM9N2F-
AsPC1	MX\DfZRwfG:6aXPpeJkh[XO\|YYm=		M{[4RVczKGi{cx?=	MlrPR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRZNRSzFiY3XscJMh[W[2ZYKgO\|IhcHK\|IHL5JG1VWyCjc4PhfUwhUUN3ME2xMlQ4\|ryP	M361W\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEW5OlE{Lz5{M{S1PVYyOzxxYU6=
PANC1	MlniR5l1d3SxeHnjbZR6KGG\|c3H5		MXq3NkBpenN?	NXHGTZh3S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hWEGQQ{GgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WUzDhd5NigSxiSVO1NF0yNjd\|zszN	M1f3N\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEW5OlE{Lz5{M{S1PVYyOzxxYU6=
MDA-MB-231	NFnRS5NCdnSrdIXtc5Ih[XO\|YYm=	NUD6RndLPzVibXevb4c>	NWXNcHhoPSCmYYnz	NWfBc45CSW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gSXIhdmWpYYTpeoUhVUSDLV3CMVI{OSClZXzsd{B5\W6xZ4Lh[pRm\CCrbjDueYRmKG2xdYPlJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[geJVud3JiZ4Lve5RpKGG2IEe1JI1oN2upLDDwc{By\CCob4KgOUBl[Xm\|	NXjTdWVYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVk3OTNpPkKzOFU6PjF\|PD;hQi=>
MDA-MB-231	M3;zSWFvfGm2dX3vdkBie3OjeR?=	MVKxNk42KG2pL3vn	NXTldGh{PSCmYYnz	NETwRYRCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDFVkBv\WejdHn2[UBOTEFvTVKtNlMyKGOnbHzzJJhmdm:pcnHmeIVlKGmwIH71[IUhdW:3c3WgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjD0eY1weiCpcn;3eIgh[XRiMUKuOUBu\y:tZzygbZAheWRiZn;yJFUh\GG7cx?=	Mn7YQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NUm2NVMoRjJ\|NEW5OlE{RC:jPh?=
MDA-MB-231	M2PTcWFvfGmycn;sbYZmemG2aY\lJIF{e2G7	MlHZNVAhfG9iMkCgeW0>	NGXXXVczPCCqcoO=	MV\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEWUIH7l[4F1cX[nIF3ERU1OSi1{M{GgZ4VtdHNiYYSgNVAhfG9iMkCgeW0h[W[2ZYKgNlQhcHK\|IHL5JG1VXCCjc4PhfS=>	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR3OU[xN{c,OjN2NUm2NVM9N2F-
MDA-MB-231	MWrGeY5kfGmxbjDhd5NigQ>?		M{[zPFI1KGi{cx?=	NVP6e5JRUW6qaXLpeIlwdiCxZjDTWGFVOyCycn;tc5RmeiCjY4Tpeol1gSCrbjDoeY1idiCHUjDu[YdifGm4ZTDNSGEuVUJvMkOxJINmdGy\|IHHmeIVzKDJ2IHjyd{BjgSCmdXHsJIx2[2moZYLhd4UhemWyb4L0[ZIh[XO\|YYm=	NXPPZlRMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVk3OTNpPkKzOFU6PjF\|PD;hQi=>
MDA-MB-231	M1TvfGZ2dmO2aX;uJIF{e2G7	NITl[IYzOCC3TR?=	M1XrTFI1KGi{cx?=	NF7FNo9KdmirYnn0bY9vKG:oIGPURXQ{KHC{b33veIVzKGGldHn2bZR6KGmwIHj1cYFvKEWUIH7l[4F1cX[nIF3ERU1OSi1{M{GgZ4VtdHNiYYSgNlAhfU1iYX\0[ZIhOjRiaILzJIJ6KGS3YXygcJVkcW[ncnHz[UBz\XCxcoTldkBie3OjeR?=	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR3OU[xN{c,OjN2NUm2NVM9N2F-
MDA-MB-231	Mny2SpVv[3Srb36gZZN{[Xl?	NI\yenIyOCC3TR?=	MYOyOEBpenN?	MojQTY5pcWKrdHnvckBw\iCVVFHUN{Bxem:vb4TldkBi[3Srdnn0fUBqdiCqdX3hckBGWiCwZXfheIl3\SCPRFGtUWIuOjNzIHPlcIx{KGG2IEGwJJVOKGGodHXyJFI1KGi{czDifUBlfWGuIHz1Z4ln\XKjc3WgdoVxd3K2ZYKgZZN{[Xl?	M2\lflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEW5OlE{Lz5{M{S1PVYyOzxxYU6=
MDA-MB-231	NXizZ3ZTTnWwY4Tpc44h[XO\|YYm=	MorjNUB1dyBzMDD1US=>	MnPHNlQhcHK\|	MXvJcohq[mm2aX;uJI9nKFOWQWSzJIlvKGi3bXHuJGVTKG6nZ3H0bZZmKE2GQT3NRk0zOzFiY3XscJMh[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIH;mJJRwfGGuIGPURXQ{KGyndnXsJIF1KDFidH:gNVAhfU1iYX\0[ZIhOjRiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?=	NFzObm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S1PVYyOyd-MkO0OVk3OTN:L3G+
MDA-MB-231	MXPGeY5kfGmxbjDhd5NigQ>?	MlTKNUB1dyBzMDD1US=>	NX7RUmxRPDhiaILz	NGTVUo1KdmS3Y4Tpc44hd2ZibX;ydIhwdG:paXPhcEBkcGGwZ3XzJIlvKGi3bXHuJGVTKG6nZ3H0bZZmKE2GQT3NRk0zOzFiY3XscJMh[XRiMTD0c{AyOCC3TTDh[pRmeiB2ODDodpMh[nlibHnnbJQhdWmlcn;zZ49xcWNiYX7hcJl{cXN?	NGGze2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S1PVYyOyd-MkO0OVk3OTN:L3G+
MDA-MB-231	NV;XR45{TnWwY4Tpc44h[XO\|YYm=	MX[xJJRwKDFyIIXN	MkD3OFghcHK\|	NU\rRldvUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDFVkBv\WejdHn2[UBOTEFvTVKtNlMyKGOnbHzzJIF1KDFidH:gNVAhfU1iYX\0[ZIhPDhiaILzJIJ6KGyrZ3j0JI1q[3Kxc3PvdIlkKGGwYXz5d4l{	NFjvdW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S1PVYyOyd-MkO0OVk3OTN:L3G+
MDA-MB-231	NEi2fGdCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=	M1fjU\|EhfG9iMUCgeW0>	MWm0PEBpenN?	NGHSVHFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFXSJI5m\2G2aY\lJG1FSS2PQj2yN\|Eh[2WubIOgZZQhOSC2bzCxNEB2VSCjZoTldkA1QCCqcoOgZpkhdGmpaISgcYlkem:\|Y3;wbYMh[W6jbInzbZM>	NWHMV4o1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVk3OTNpPkKzOFU6PjF\|PD;hQi=>
MDA-MB-231	NHTydndHfW6ldHnvckBie3OjeR?=	Mlm0NUB1dyBzMDD1US=>	MnntNlQhcHK\|	MmnjTY5pcWKrdHnvckBw\iCVVFHUN{BqdiCqdX3hckBGWiCwZXfheIl3\SCPRFGtUWIuOjNzIHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDv[kBxcG:\|cHjvdplt[XSnZDDTWGFVOyCjdDDUfZIuPzB3IHzleoVtKGG2IEGgeI8hOTBidV2gZYZ1\XJiMkSgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=>	M1jZNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEW5OlE{Lz5{M{S1PVYyOzxxYU6=
HeLa	MX;GeY5kfGmxbjDhd5NigQ>?		MVWyOEBpenN?	NXnrdHVMUW6qaXLpeIlwdiCxZjDTWGFVOyCrbjDoeY1idiCKZVzhJINmdGy\|IHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGlEPTB;MD6yOe69VQ>?	M{ji[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwNlMyLz5{NEmwNFI{OTxxYU6=
DU145	MmTjRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NWHOR2dYPzJiaILz	MlzTRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCGVUG0OUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRTBwN988US=>	MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEKzNUc,OjR7MECyN\|E9N2F-
DU145	MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		MlzNNVEhfG9iMUKg[IF6ew>?	MWrHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDEWVE1PSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJINwdG:weTDmc5Ju[XSrb36gZYZ1\XJiMUGgeI8hOTJiZHH5d{BjgSClconzeIFtKH[rb3zleEB{fGGrbnnu[{Bj[XOnZDDtbYNzd3Olb4DpZ{BidmGueYPpd{whUUN3ME2wMlfPxE1?	NWnKV\|BGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFAzOzFpPkK0PVAxOjNzPD;hQi=>
HeLa	MX;BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		MXm3NkBpenN?	NEKwd49CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjlUIEh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zLkVOwG0>	NGXU[2U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFI{OSd-MkS5NFAzOzF:L3G+
A549	M1zIWGFvfGmycn;sbYZmemG2aY\lJIF{e2G7		NIrwe\|U4OiCqcoO=	MWLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF3NEmgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0{\|ryP	MlztQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MECyN\|EoRjJ2OUCwNlMyRC:jPh?=
HT-29	M{DYNmFvfGmycn;sbYZmemG2aY\lJIF{e2G7		MnjEO\|IhcHK\|	NH34WWhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjUMVI6KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;Nz6y{txO	Mn7UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MECyN\|EoRjJ2OUCwNlMyRC:jPh?=
A431	M1vXV2FvfGmycn;sbYZmemG2aY\lJIF{e2G7		NE\SN4c4OiCqcoO=	NUHjPGJtSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOFMyKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;OD64{txO	NIS4N\|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFI{OSd-MkS5NFAzOzF:L3G+
PC3	MlizRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NX7McYd6PzJiaILz	NYfrZpV[SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDQR\|Mh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zMT63{txO	MorQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MECyN\|EoRjJ2OUCwNlMyRC:jPh?=
HeLa	M3O3eWZ2dmO2aX;uJIF{e2G7	MoG3OUB2VQ>?	M{Xx[VI1KGi{cx?=	NYnH[JB6UW6qaXLpeIlwdiCxZjDTWGFVOyCrbjDoeY1idiCKZVzhJINmdGy\|IHH0JFUhfU1iYX\0[ZIhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeR?=	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEKzNUc,OjR7MECyN\|E9N2F-
DU145	MoTNSpVv[3Srb36gZZN{[Xl?	MkfZNUB2VQ>?	MoHENkBpenN?	MWrJcohq[mm2aX;uJI9nKFOWQWSzJI52[2ynYYKgeJJidnOub3PheIlwdiCrbjDFS2Yhe3SrbYXsZZRm\CCqdX3hckBFXTF2NTDj[YxteyCjdDCxJJVOKGGmbXnubZN1\XKnZDDi[YZwemViMUCwJI5oN22uIFXHSkB{fGmvdXzheIlwdiCvZXHzeZJm\CCjZoTldkAzKGi{czDifUBkd26ob3PhcEBt[XOncjDtbYNzd3Olb4D5	MkLLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MECyN\|EoRjJ2OUCwNlMyRC:jPh?=
DU145	NF[2RnBHfW6ldHnvckBie3OjeR?=	NIn2fZoxPSC2bzCxNEB2VQ>?	MkfONkBpenN?	NFK3V\|hKdmirYnn0bY9vKG:oIGPURXQ{KGmwdHXyZYN1cW:wIIfpeIghTE6DIHLpcoRqdmdic3n0[UBqdiCqdX3hckBFXTF2NTDj[YxteyCjdDCwOUB1dyBzMDD1UUBi\nSncjCyJIhzeyCkeTDFUXNC	M3vzXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwNlMyLz5{NEmwNFI{OTxxYU6=
human	Mme1SpVv[3Srb36gZZN{[Xl?	NGrYe2ExNjFidH:gNkB2VQ>?	M1fqflIhcHK\|	MVTE[YNz\WG\|ZTDpckBkgWOuaX6gSFEheHKxdHXpckBt\X[nbDDpckBpfW2jbjDEWVE1PSClZXzsd{BifCByLkGgeI8hOiC3TTDh[pRmeiB{IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm\|aYO=	Mn\QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MECyN\|EoRjJ2OUCwNlMyRC:jPh?=
human	M13sUGZ2dmO2aX;uJIF{e2G7	Mn;SNE4yKHSxIEKgeW0>	NYj3UXV7OiCqcoO=	MVPE[YNz\WG\|ZTDpckBkNU27YzDwdo91\WmwIHzleoVtKGmwIHj1cYFvKESXMUS1JINmdGy\|IHH0JFAvOSC2bzCyJJVOKGGodHXyJFIhcHK\|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>?	M{nVZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwNlMyLz5{NEmwNFI{OTxxYU6=
human	NGHMS2RHfW6ldHnvckBie3OjeR?=	M37u[FAvOSC2bzCyJJVO	M4fYUlIhcHK\|	NEmyWVNF\WO{ZXHz[UBqdiCEY3ytXIwheHKxdHXpckBt\X[nbDDpckBpfW2jbjDEWVE1PSClZXzsd{BifCByLkGgeI8hOiC3TTDh[pRmeiB{IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm\|aYO=	NHji[Vk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFI{OSd-MkS5NFAzOzF:L3G+
DU145	NELrXXVHfW6ldHnvckBie3OjeR?=	NWXt[ngxOC5zIITvJFIhfU1?	MVeyJIhzew>?	M3fyNGRwf26{ZXf1cIF1cW:wIH;mJG1kdC1zIIDyc5RmcW5iaX6gbJVu[W5iRGWxOFUh[2WubIOgZZQhOC5zIITvJFIhfU1iYX\0[ZIhOiCqcoOgZpkhX2W\|dHXyckBjdG:2IHHuZYx6e2m\|	NF7ObZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFI{OSd-MkS5NFAzOzF:L3G+
human	NGD3Z2pHfW6ldHnvckBie3OjeR?=	M3rsd\|IhfU1?	M4PqdFIhcHK\|	M1fuTWlvcGmkaYTpc44hd2ZiU2TBWFMheGixc4Doc5J6dGG2aX;uJIF1KFS7ckewOUBqdiCqdX3hckBFXTF2NTDj[YxteyCjdDCyJJVOKHerdHjpckAzKGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN?	M1GyeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwNlMyLz5{NEmwNFI{OTxxYU6=
breast cancer cells	NIL4RW9EgXSxdH;4bYNqfHliYYPzZZk>		NH3KXJY4OiCqcoO=	NUj3[nFpS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4h[nKnYYP0JINidmOncjDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB5MjDodpMh[nliQ3XscEBVcXSncjDHcI8hSXO\|YYmsJGlEPTB;MD6xN:69VQ>?	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ5MkCzNkc,OjZ{N{KwN\|I9N2F-
colon cancer cells	NVnTToJKS3m2b4TvfIlkcXS7IHHzd4F6		M2rYR\|czKGi{cx?=	NEmyc\|BEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBkd2yxbjDjZY5k\XJiY3XscJMh[XO\|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPzJiaILzJIJ6KEOnbHygWIl1\XJiR3zvJGF{e2G7LDDJR\|UxRTBwMUROwG0>	MkDOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{N{KwN\|IoRjJ4MkeyNFMzRC:jPh?=
lung cancer cells	M3rjOGN6fG:2b4jpZ4l1gSCjc4PhfS=>		MYm3NkBpenN?	NVLmVJBxS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hdHWwZzDjZY5k\XJiY3XscJMh[XO\|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPzJiaILzJIJ6KEOnbHygWIl1\XJiR3zvJGF{e2G7LDDJR\|UxRTBwMUROwG0>	NEKzeJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkK3NlA{Oid-Mk[yO\|IxOzJ:L3G+
prostate cancer cells	MmXsR5l1d3SxeHnjbZR6KGG\|c3H5		NIL4R404OiCqcoO=	M1:yc2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJJBzd3O2YYTlJINidmOncjDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB5MjDodpMh[nliQ3XscEBVcXSncjDHcI8hSXO\|YYmsJGlEPTB;MD6xN:69VQ>?	NVLCUZFCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[yO\|IxOzJpPkK2NlczODN{PD;hQi=>
ovarian cancer cells	M1PUTmN6fG:2b4jpZ4l1gSCjc4PhfS=>		MYK3NkBpenN?	M33R[GN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJI93[XKrYX6gZ4Fv[2W{IHPlcIx{KGG\|c3Xzd4VlKGG\|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDd{IHjyd{BjgSCFZXzsJHRqfGW{IFfsc{BCe3OjeTygTWM2OD1yLkGz{txO	NIPXWGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkK3NlA{Oid-Mk[yO\|IxOzJ:L3G+
blood cancer cells	MUXDfZRwfG:6aXPpeJkh[XO\|YYm=		Mmr0O\|IhcHK\|	M{fzUWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJIJtd2:mIHPhcoNmeiClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA4OiCqcoOgZpkhS2WubDDUbZRmeiCJbH:gRZN{[XluIFnDOVA:OC5zM988US=>	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ5MkCzNkc,OjZ{N{KwN\|I9N2F-
pancreatic cancer cells	M1Lw[WN6fG:2b4jpZ4l1gSCjc4PhfS=>		M3Thc\|czKGi{cx?=	NWH1R4hyS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4heGGwY4LlZZRq[yClYX7j[ZIh[2WubIOgZZN{\XO\|ZXSgZZMh\3Kxd4ToJIlvcGmkaYTpc44h[W[2ZYKgO\|IhcHK\|IHL5JGNmdGxiVHn0[ZIhT2yxIFHzd4F6NCCLQ{WwQVAvOTQQvF2=	NHznT5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkK3NlA{Oid-Mk[yO\|IxOzJ:L3G+
HEK293	NVfhN4JrTnWwY4Tpc44h[XO\|YYm=		MlHDPEBpenN?	NFTLdmVKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5iaX6gTGVMOjl\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnubIljcXSrb36gc4YhX262M1Gtd5RqdXWuYYTl[EBVV1CIbHHzbEBi[3Srdnn0fUBi\nSncjC4JIhzeyxiSVO1NF0xNjN2zszN	NHTzfYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkK3NlA{Oid-Mk[yO\|IxOzJ:L3G+
HCT116	NG\oXpBEgXSxdH;4bYNqfHliYYPzZZk>		M4XRSVczKGi{cx?=	M{LCemN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDFzNjDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB5MjDodpMh[nliTWTTJIF{e2G7LDDJR\|UxRTBwNEZOwG0>	M4jsdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MkeyNFMzLz5{NkK3NlA{OjxxYU6=
U2OS	M1vOWWZ2dmO2aX;uJIF{e2G7	NEjGR4kyOi53IIXN	MU[2JIhzew>?	NGLCellKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5iaX6gbJVu[W5iVULPV{Bk\WyuczDhd5Nme3OnZDDhd{BqdnSncn7hcIl7[XSrb36gc4Yh\nKreors[YQuT0[SIHH0JFEzNjVidV2gZYZ1\XJiNjDodpMh[nliY3;u[o9k[WxibXnjdo9{[2:yaXOgZY5idHm\|aYO=	MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ5MkCzNkc,OjZ{N{KwN\|I9N2F-
HCT116	MWDGeY5kfGmxbjDhd5NigQ>?		MWOzJIhzew>?	NIDv[WJG\m[nY4Sgc44hSVSSIHjvcYVwe3Sjc3nzJIlvKGi3bXHuJGhEXDFzNjDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hSVSSIHzleoVtKGGodHXyJFMhcHK\|IHnuJIFje2WwY3Wgc4Yh\2y3Y3;z[UBjgSCDVGCgZolwdHWvaX7ld4NmdnRiYYPzZZktKEWFNUC9NE4xPTcQvF2=	NIC4bFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEKzN\|Y5OCd-MkiyN\|M3QDB:L3G+
HEK293	MVrGeY5kfGmxbjDhd5NigQ>?		M33PNlYhcHK\|	NITSNWVKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5ic3nncoFtcW6pIDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKFewdEPBMZN1cW23bHH0[YQh[mW2YT3jZZRmdmmwIILld5BwdnOnIITyZY5{[3KrcITpc44h[W[2ZYKgOkBpenNiYomgWG9R\myjc3ig[JVidCCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGlEPTB;MD6xNu69VQ>?	NHXDZ4g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEKzN\|Y5OCd-MkiyN\|M3QDB:L3G+
HCT116	MnrnSpVv[3Srb36gZZN{[Xl?		MkXmN{BpenN?	NFn2[4RG\m[nY4Sgc44hSVSSIHjvcYVwe3Sjc3nzJIlvKGi3bXHuJGhEXDFzNjDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hSVSSIHzleoVtKGGodHXyJFMhcHK\|IHnuJIFje2WwY3Wgc4Yh\2y3Y3;z[UBjgSCDVGCgZolwdHWvaX7ld4NmdnRiYYPzZZktKEmFNUC9NE4zPc7:TR?=	M4TCeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkOzOlgxLz5{OEKzN\|Y5ODxxYU6=
HCT116	NUnReohJTnWwY4Tpc44h[XO\|YYm=	MXO1JJVO	MX[xPEBpenN?	M2fBXGlvcGmkaYTpc44hd2ZiV370M4JmfGFvY3H0[Y5qdiC\|aXfuZYxqdmdiaX6gbJVu[W5iSFPUNVE3KGOnbHzzJIF{e2W\|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKGO7dH;zc4xq[yCkZYThMYNifGWwaX6gdJJwfGWrbjDlfJBz\XO\|aX;uJIF1KDVidV2gZYZ1\XJiMUigbJJ{KGK7IGfld5Rmem5iYnzveEBu\XSqb3S=	NY\lSmFKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiyN\|M3QDBpPkK4NlM{PjhyPD;hQi=>
HCT116	NWmx[VZHTnWwY4Tpc44h[XO\|YYm=	NH;qfVA2KHWP	NH3aPIUyQCCqcoO=	M1XPdGlvcGmkaYTpc44hd2ZiV370M4JmfGFvY3H0[Y5qdiC\|aXfuZYxqdmdiaX6gbJVu[W5iSFPUNVE3KGOnbHzzJIF{e2W\|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKGO7dH;zc4xq[yCDeHnuNkBxem:2ZXnuJIV5eHKnc4Ppc44h[XRiNTD1UUBi\nSncjCxPEBpenNiYomgW4V{fGW{bjDicI91KG2ndHjv[C=>	Ml\RQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{M{O2PFAoRjJ6MkOzOlgxRC:jPh?=
HCT116	NWDsNo9rTnWwY4Tpc44h[XO\|YYm=	NX7peotsPSC3TR?=	MY[xPEBpenN?	NIL6[4tKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5ic3nncoFtcW6pIHnuJIh2dWGwIFjDWFEyPiClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iClLV35Z{Bxem:2ZXnuJIV5eHKnc4Ppc44h[XRiNTD1UUBi\nSncjCxPEBpenNiYomgW4V{fGW{bjDicI91KG2ndHjv[C=>	MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDJ\|M{[4NEc,Ojh{M{O2PFA9N2F-
HCT116	MX3GeY5kfGmxbjDhd5NigQ>?	MVu1JJVO	NH;pZYgyQCCqcoO=	NF\JcHJKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5ic3nncoFtcW6pIHnuJIh2dWGwIFjDWFEyPiClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iCFeXPsbY4hTDFicILveIVqdiCneIDy[ZN{cW:wIHH0JFUhfU1iYX\0[ZIhOThiaILzJIJ6KFenc4Tldo4h[myxdDDt[ZRpd2R?	MlryQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{M{O2PFAoRjJ6MkOzOlgxRC:jPh?=
HCT116	MV\GeY5kfGmxbjDhd5NigQ>?	MVq1JJVO	NWq4S4h7OThiaILz	NX3nc25wUW6qaXLpeIlwdiCxZjDXcpQw[mW2YT3jZZRmdmmwIIPp[45idGmwZzDpckBpfW2jbjDIR3QyOTZiY3XscJMh[XO\|ZYPz[YQh[XNiZH;3cpJm\3WuYYTpc44hd2Zic4Xyeol3cW5icILveIVqdiCneIDy[ZN{cW:wIHH0JFUhfU1iYX\0[ZIhOThiaILzJIJ6KFenc4Tldo4h[myxdDDt[ZRpd2R?	NGX2Rpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEKzN\|Y5OCd-MkiyN\|M3QDB:L3G+
SW480	MmfGSpVv[3Srb36gZZN{[Xl?	MXe1JJVO	NUTEU3F1OThiaILz	MVTJcohq[mm2aX;uJI9nKFewdD;i[ZRiNWOjdHXubY4he2mpbnHsbY5oKGmwIHj1cYFvKFOZNEiwJINmdGy\|IHHzd4V{e2WmIHHzJIRwf26{ZXf1cIF1cW:wIH;mJIN6fG:\|b3zpZ{Bj\XSjLXPheIVvcW5icILveIVqdiCneIDy[ZN{cW:wIHH0JFUhfU1iYX\0[ZIhOThiaILzJIJ6KFenc4Tldo4h[myxdDDt[ZRpd2R?	M2jGTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkOzOlgxLz5{OEKzN\|Y5ODxxYU6=
SW480	NX:5dW0yTnWwY4Tpc44h[XO\|YYm=	NG\hT4M2KHWP	M3jISFE5KGi{cx?=	NWnJc3g{UW6qaXLpeIlwdiCxZjDXcpQw[mW2YT3jZZRmdmmwIIPp[45idGmwZzDpckBpfW2jbjDTW\|Q5OCClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iCleYTvd49tcWNiQYjpclIheHKxdHXpckBmgHC{ZYPzbY9vKGG2IEWgeW0h[W[2ZYKgNVghcHK\|IHL5JHdme3Sncn6gZoxwfCCvZYToc4Q>	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDJ\|M{[4NEc,Ojh{M{O2PFA9N2F-
SW480	NEn4bVZHfW6ldHnvckBie3OjeR?=	NInwd2M2KHWP	NXzlU5F{OThiaILz	M{\2[GlvcGmkaYTpc44hd2ZiV370M4JmfGFvY3H0[Y5qdiC\|aXfuZYxqdmdiaX6gbJVu[W5iU2e0PFAh[2WubIOgZZN{\XO\|ZXSgZZMh\G:5boLl[5Vt[XSrb36gc4Yh[y2PeXOgdJJwfGWrbjDlfJBz\XO\|aX;uJIF1KDVidV2gZYZ1\XJiMUigbJJ{KGK7IGfld5Rmem5iYnzveEBu\XSqb3S=	NEjuUnQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEKzN\|Y5OCd-MkiyN\|M3QDB:L3G+
SW480	NVL5VVMyTnWwY4Tpc44h[XO\|YYm=	NYPSeGxvPSC3TR?=	MkDFNVghcHK\|	M2rLT2lvcGmkaYTpc44hd2ZiV370M4JmfGFvY3H0[Y5qdiC\|aXfuZYxqdmdiaX6gbJVu[W5iU2e0PFAh[2WubIOgZZN{\XO\|ZXSgZZMh\G:5boLl[5Vt[XSrb36gc4YhS3mlbHnuJGQyKHC{b4TlbY4h\XiycnXzd4lwdiCjdDC1JJVOKGGodHXyJFE5KGi{czDifUBY\XO2ZYLuJIJtd3RibXX0bI9l	MmnhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{M{O2PFAoRjJ6MkOzOlgxRC:jPh?=
SW480	MUfGeY5kfGmxbjDhd5NigQ>?	NVH0N4RlPSC3TR?=	NWD2PJFvOThiaILz	MYTJcohq[mm2aX;uJI9nKFewdD;i[ZRiNWOjdHXubY4he2mpbnHsbY5oKGmwIHj1cYFvKFOZNEiwJINmdGy\|IHHzd4V{e2WmIHHzJIRwf26{ZXf1cIF1cW:wIH;mJJN2en[rdnnuJJBzd3SnaX6g[ZhxemW\|c3nvckBifCB3IIXNJIFnfGW{IEG4JIhzeyCkeTDX[ZN1\XKwIHLsc5QhdWW2aH;k	MnTNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{M{O2PFAoRjJ6MkOzOlgxRC:jPh?=
HCT116	M{CwWWZ2dmO2aX;uJIF{e2G7	NHXFVpkzKHWP	MWezNEBucW6\|	NH7Rc5pKdmS3Y4Tpc44hd2ZiQV3QT{BxcG:\|cHjvdplt[XSrb36gZZQhXGi{LUG3NkBz\XOrZIXlJIlvKGi3bXHuJGhEXDFzNjDj[YxteyCjdDCyJJVOKGGodHXyJFMxKG2rboOgbY4h\2y3Y3;z[UB{fXCybHXt[Y51\WRibXXkbYEh[nliaX3teY5w[myxdDDt[ZRpd2R?	MkTqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{M{O2PFAoRjJ6MkOzOlgxRC:jPh?=
HCT116	NWnCR4hrTnWwY4Tpc44h[XO\|YYm=	MVyyJJVO	NGPvRpI{OCCvaX7z	MYHJcoR2[3Srb36gc4YhSU2SSzDwbI9{eGixconsZZRqd25iYYSgWIhzNTF5MjDy[ZNq\HWnIHnuJIh2dWGwIFjDWFEyPiClZXzsd{BifCB{IIXNJIFnfGW{IEOwJI1qdnNiaX6gZYJ{\W6lZTDv[kBodHWlb4PlJIJ6KGmvbYXuc4Jtd3RibXX0bI9l	NIPXTlM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEKzN\|Y5OCd-MkiyN\|M3QDB:L3G+
U2OS	NGHLfWZCdnSrdnnyZYwh[XO\|YYm=			NX;xO\|hKSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhS2ira4Xu[5VvgWFidnnyeZMhcW6oZXP0[YQhcW5iaIXtZY4hXTKRUzDj[YxteyCkeTDSWE1yWEOUIHHuZYx6e2m\|LDDFR\|UxRTBwM{dOwG0>	M{G1VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6Nki5PVc2Lz5{OE[4PVk4PTxxYU6=
BHK-21	MkS1RY51cX[rcnHsJIF{e2G7			MV;BcpRqfmm{YXygZYN1cX[rdImgZYdicW6\|dDDDbIlsfW6pdX75ZUB3cXK3czCwOlEy[VS5IHnu[oVkfGWmIHnuJGJJUy1{MTDj[YxteyCkeTDSWE1yWEOUIHHuZYx6e2m\|LDDFR\|UxRTBwOEZOwG0>	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDZ6OUm3OUc,Ojh4OEm5O\|U9N2F-
BHK-21	MlrMRY51cX[rcnHsJIF{e2G7			NYnaW\|JjSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhS2ira4Xu[5VvgWFidnnyeZMhODhzMHLUe{Bqdm[nY4Tl[EBqdiCESFutNlEh[2WubIOgZpkhWlRvcWDDVkBidmGueYPpd{whTUN3ME2wMlnPxE1?	NFXoe4M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[4PVk4PSd-Mki2PFk6PzV:L3G+
BHK-21	MV\BcpRqfmm{YXygZZN{[Xl?			MY\BcpRqfmm{YXygZYN1cX[rdImgZYdicW6\|dDDDbIlsfW6pdX75ZUB3cXK3czDpcoZm[3SnZDDpckBDUEtvMkGgZ4VtdHNiYomgVnQueVCFUjDhcoFtgXOrczygSWM2OD1yLkm1{txO	NGnMU4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[4PVk4PSd-Mki2PFk6PzV:L3G+
DAOY	NEXKUphyUFSVIHHzd4F6			NELvTIRyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiRFHPXUBk\Wyucx?=	NWDGRnhnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
SJ-GBM2	MXfxTHRUKGG\|c3H5			NXjs[\|NVeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IGPKMWdDVTJiY3XscJM>	NX\rblFMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
A673	Mk[xdWhVWyCjc4PhfS=>			MlvidWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKEF4N{OgZ4VtdHN?	Ml;OQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC	MXfxTHRUKGG\|c3H5			MVfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?=	MmjLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
BT-37	NFrMcVhyUFSVIHHzd4F6			Mnq3dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKEKWLUO3JINmdGy\|	M33YOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB-EBc1	NIfBeWtyUFSVIHHzd4F6			NGDpfoVyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTlKtSWJkOSClZXzsdy=>	M2LCbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Saos-2	NELz[YRyUFSVIHHzd4F6			MUjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW2Gxcz2yJINmdGy\|	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
SK-N-SH	MYLxTHRUKGG\|c3H5			MVrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3TTEBk\Wyucx?=	MoThQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
NB1643	MV;xTHRUKGG\|c3H5			M1vUUJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCQQkG2OFMh[2WubIO=	M4O2OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
LAN-5	NFPse\|ZyUFSVIHHzd4F6			MUnxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVEGQLUWgZ4VtdHN?	MkLnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
BT-12	MkT4dWhVWyCjc4PhfS=>			NEjDNoRyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQmStNVIh[2WubIO=	M{PuRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh18	NH[4PG1yUFSVIHHzd4F6			M{iydpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCUaEG4JINmdGy\|	MkDiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
OHS-50	NUDUfpc3eUiWUzDhd5NigQ>?			MnjodWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKE:KUz21NEBk\Wyucx?=	MoruQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
RD	NV7QRm4xeUiWUzDhd5NigQ>?			MofVdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKFKGIHPlcIx{	MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
MG 63 (6-TG R)	MkX4dWhVWyCjc4PhfS=>			MU\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVUdiNkOgLFYuXEdiUjmgZ4VtdHN?	Ml7vQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
Rh41	M1v5U5FJXFNiYYPzZZk>			NFHyWXFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUni0NUBk\Wyucx?=	MnPmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
NB1643	M{\CZZFJXFNiYYPzZZk>			MoX1dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUmIyPjR\|IHPlcIx{	MnLiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
A673	MoXjdWhVWyCjc4PhfS=>			NY\BZWJEeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhSTZ5MzDj[Yxteyl?	MoHnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC	MYTxTHRUKGG\|c3H5			MXjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTT{1PNU2FIHPlcIx{	MoTnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
BT-12	Mlq3dWhVWyCjc4PhfS=>			MmDOdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgRnQuOTJiY3XscJM>	MmjsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
LAN-5	NE\SUHlyUFSVIHHzd4F6			NXmwZ5lpeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhVEGQLUWgZ4VtdHN?	MlGwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
DAOY	MoO1dWhVWyCjc4PhfS=>			M4rYcpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJGRCV1liY3XscJM>	M3LxXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB-EBc1	MmDNdWhVWyCjc4PhfS=>			Mln2dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUmIuTUKlMTDj[Yxtew>?	MkG5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
SJ-GBM2	NVnycY5YeUiWUzDhd5NigQ>?			NUK1eI9ZeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhW0pvR1LNNkBk\Wyucx?=	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
BT-37	MWLxTHRUKGG\|c3H5			MXrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDCWE0{PyClZXzsdy=>	M2rtZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
TC32	MnfWdWhVWyCjc4PhfS=>			MmrQdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgWGM{OiClZXzsdy=>	NVvocYRJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
MG 63 (6-TG R)	MmTodWhVWyCjc4PhfS=>			MWTxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDNS{A3OyBqNj3US{BTMSClZXzsdy=>	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
fibroblast cells	MUHxTHRUKGG\|c3H5			NGO0W3lyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBkd262cn;sJGhpKHerbHSgeJlx\SCoaXLyc4Jt[XO2IHPlcIx{	MmK1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
U-2 OS	NW\IeoZFeUiWUzDhd5NigQ>?			M3T5U5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHUuOiCRUzDj[Yxtew>?	M1zYXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh41	M1HZWpFJXFNiYYPzZZk>			MVXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDSbFQyKGOnbHzz	MkDPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
RD	MofqdWhVWyCjc4PhfS=>			MYfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDSSEBk\Wyucx?=	MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
Rh18	NXi3VWRneUiWUzDhd5NigQ>?			NH32WllyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBTcDF6IHPlcIx{	MofSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
Rh30	M{LjTpFJXFNiYYPzZZk>			NX3YNpNleUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhWmh\|MDDj[Yxtew>?	NYHqNGZbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
Saos-2	NW\XbHZneUiWUzDhd5NigQ>?			NHnaPWhyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBU[W:\|LUKgZ4VtdHN?	M2DO[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
OHS-50	M4TEcpFJXFNiYYPzZZk>			NX:xRnNueUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy\|	NVjHWXdkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-SH	MoPpdWhVWyCjc4PhfS=>			M4T0V5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHNMNU5vU1igZ4VtdHN?	M{LB[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-SH	MonWdWhVWyCjc4PhfS=>			NFWz[XpyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCRcoToc4dwdmGuIEPEJJZq[WKrbHn0fUB{[3KnZX6g[o9zKFONLV6tV2gh[2WubIO=	MoT4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
SJ-GBM2	M4jZTZFJXFNiYYPzZZk>			NFH1Z5pyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCRcoToc4dwdmGuIEPEJJZq[WKrbHn0fUB{[3KnZX6g[o9zKFOMLVfCUVIh[2WubIO=	NH\1fII9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
Daoy	MVnxTHRUKGG\|c3H5			NUDN[WdreUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiT4L0bI9od26jbDCzSEB3cWGkaXzpeJkhe2O{ZXXuJIZweiCGYX;5JINmdGy\|	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
TC32	MkXQdWhVWyCjc4PhfS=>			M4D6[pFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KE:{dHjv[49v[WxiM1Sgeoli[mmuaYT5JJNkemWnbjDmc5IhXEN\|MjDj[Yxtew>?	M1:1PVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
MG 63 (6-TG R)	NVjPSIhTeUiWUzDhd5NigQ>?			NXfpS3hLeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiT4L0bI9od26jbDCzSEB3cWGkaXzpeJkhe2O{ZXXuJIZweiCPRzC2N{ApPi2WRzDSLUBk\Wyucx?=	MnXXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
RD	MlyydWhVWyCjc4PhfS=>			NFflPFdyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCRcoToc4dwdmGuIEPEJJZq[WKrbHn0fUB{[3KnZX6g[o9zKFKGIHPlcIx{	NEjUNXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
SW948	NVX3Z41bSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		MYC3NkBpenN?	MlLVRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCVV{m0PEBk\WyuczDh[pRmeiB5MjDodpMh[nliY3;sc5JqdWW2cnnjJG1VWyCjc4PhfUwhUUN3ME2wMlEy\|ryP	MoPtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{N{S5N\|koRjNyMke0PVM6RC:jPh?=
HT-29	MojzRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NH\DT4Q4OiCqcoO=	MVLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiWLUK5JINmdGy\|IHHmeIVzKDd{IHjyd{BjgSClb3zvdolu\XS{aXOgUXRUKGG\|c3H5MEBKSzVyPUCuNVPPxE1?	NUT4OZF[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyO\|Q6OzlpPkOwNlc1QTN7PD;hQi=>
HCT116	Mln4RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MXi3NkBpenN?	Mlr6RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCKQ2SxNVYh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IHPvcI9zcW2ndILpZ{BOXFNiYYPzZZktKEmFNUC9NE41Oc7:TR?=	NHXTRnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEK3OFk{QSd-M{CyO\|Q6Ozl:L3G+
HEK293	Mo\ySpVv[3Srb36gZZN{[Xl?		NIjmbpI3KGi{cx?=	NIfYZ2RKdmirYnn0bY9vKG:oIGfueFNCNXO2aX31cIF1\WRiV370M4JmfGFvY3H0[Y5qdiCyYYToe4F6KGmwIFjFT\|I6OyClZXzsd{B1emGwc3\lZ5Rm\CC5aYToJJA5gFSRUF\sZZNpNCCUZX7pcIxiKGy3Y3nm[ZJie2VicHzhd41q\CCyUlytWGsh[W6mIIDMT28vOSCjZoTldkA3KGi{czDifUBFfWGuIITvdIZt[XOqIHz1Z4ln\XKjc3WgdoVxd3K2ZYKgZZN{[XluIFnDOVA:OC52Nd88US=>	M4rY[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMke0PVM6Lz5\|MEK3OFk{QTxxYU6=
CRC240	MonVRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		M{nHVFczKGi{cx?=	M{TUXmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQ2LDNlQxKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDjc4xwemmvZYTybYMhVVSVIHHzd4F6NCCLQ{WwQVAvQDoQvF2=	NYmxNHhjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyO\|Q6OzlpPkOwNlc1QTN7PD;hQi=>
SW480	MWTBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NGHjSYM4OiCqcoO=	M3\iTWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU2e0PFAh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IHPvcI9zcW2ndILpZ{BOXFNiYYPzZZktKEmFNUC9NE46QM7:TR?=	M4juRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMke0PVM6Lz5\|MEK3OFk{QTxxYU6=
DLD1	MXHBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NFrlRXM4OiCqcoO=	MUTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKESOREGgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGOxbH;ybY1mfHKrYzDNWHMh[XO\|YYmsJGlEPTB;Mj6zPe69VQ>?	NXHFdYdNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyO\|Q6OzlpPkOwNlc1QTN7PD;hQi=>
HCT116	NUS3O4tmTnWwY4Tpc44h[XO\|YYm=	M4TqblUhfU1?	MXSxPEBpenN?	MWnJcohq[mm2aX;uJI9nKFewdD;i[ZRiNWOjdHXubY4he2mpbnHsbY5oKHCjdHj3ZZkhcW5iaIXtZY4hUEOWMUG2JINmdGy\|IHP5eI9{d2xibInzZZRmKGijcnLvdolv\yCkZYThMYNifGWwaX6gcZV1[XSrb36gZZN{\XO\|ZXSgZZMhemWmdXP0bY9vKGmwIFH4bY4zKHC{b4TlbY4h\XiycnXzd4lwdiCjdDC1JJVOKGGodHXyJFE5KGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN?	NXfLeVRqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyO\|Q6OzlpPkOwNlc1QTN7PD;hQi=>
HCT116	NGf3NnlHfW6ldHnvckBie3OjeR?=	M3;GUlUhfU1?	NEXWUo8yQCCqcoO=	NVPq[\|RiUW6qaXLpeIlwdiCxZjDXcpQw[mW2YT3jZZRmdmmwIIPp[45idGmwZzDwZZRpf2G7IHnuJIh2dWGwIFjDWFEyPiClZXzsd{BkgXSxc3;sJIx6e2G2ZTDoZZJjd3KrbnegZoV1[S2lYYTlcolvKG23dHH0bY9vKGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBj\XSjLXPheIVvcW5icILveIVqdiCneIDy[ZN{cW:wIHH0JFUhfU1iYX\0[ZIhOThiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?=	NWPhWHg6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyO\|Q6OzlpPkOwNlc1QTN7PD;hQi=>
SW480	NYfLR5d5TnWwY4Tpc44h[XO\|YYm=	NFu4[ZI2KHWP	MXexPEBpenN?	M2jwO2lvcGmkaYTpc44hd2ZiV370M4JmfGFvY3H0[Y5qdiC\|aXfuZYxqdmdicHH0bJdigSCrbjDoeY1idiCVV{S4NEBk\WyuczDjfZRwe2:uIHz5d4F1\SCqYYLic5JqdmdiQWDDJI12fGG2aX;uJIF{e2W\|c3XkJIF{KHKnZIXjeIlwdiCrbjDBfIlvOiCycn;0[YlvKGW6cILld5Nqd25iYYSgOUB2VSCjZoTldkAyQCCqcoOgZpkhX2W\|dHXyckBjdG:2IHHuZYx6e2m\|	MnvMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{N{S5N\|koRjNyMke0PVM6RC:jPh?=
SW480	M3rrdmZ2dmO2aX;uJIF{e2G7	M{TnSVUhfU1?	NWj2ZnJ6OThiaILz	NF;RbWtKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5ic3nncoFtcW6pIIDheIh4[XliaX6gbJVu[W5iU2e0PFAh[2WubIOgZ5l1d3OxbDDsfZNifGViaHHyZo9zcW6pIFHQR{BufXSjdHnvckBie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZoV1[S2lYYTlcolvKHC{b4TlbY4h\XiycnXzd4lwdiCjdDC1JJVOKGGodHXyJFE5KGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN?	NX\me4xNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyO\|Q6OzlpPkOwNlc1QTN7PD;hQi=>
CRC240	MYnGeY5kfGmxbjDhd5NigQ>?	NIfLNWE2KHWP	MYmxPEBpenN?	Mnu1TY5pcWKrdHnvckBw\iCZboSvZoV1[S2lYYTlcolvKHOrZ37hcIlv\yCyYYToe4F6KGmwIHj1cYFvKEOUQ{K0NEBk\WyuczDjfZRwe2:uIHz5d4F1\SCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hSXirbkKgdJJwfGWrbjDlfJBz\XO\|aX;uJIF1KDVidV2gZYZ1\XJiMUigbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=>	M4H4WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMke0PVM6Lz5\|MEK3OFk{QTxxYU6=
CRC240	MoXxSpVv[3Srb36gZZN{[Xl?	NVXFeZVrPSC3TR?=	MUmxPEBpenN?	NGH6PYxKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5ic3nncoFtcW6pIIDheIh4[XliaX6gbJVu[W5iQ2LDNlQxKGOnbHzzJIN6fG:\|b3ygcJl{[XSnIHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCkZYThMYNifGWwaX6gdJJwfGWrbjDlfJBz\XO\|aX;uJIF1KDVidV2gZYZ1\XJiMUigbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=>	M{LGS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMke0PVM6Lz5\|MEK3OFk{QTxxYU6=
CCD-841-CoN	M13WWmZ2dmO2aX;uJIF{e2G7	NGTsOFIyKHWP		MmPTV5RqdXWuYYTpc44hd2ZibXn0c4Npd26mcnnhcEBz\XOyaYLheIlwdiCrbjDoeY1idiCFQ1StPFQyNUOxTjDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckBwgHmpZX6gZ49ve3WvcITpc44hemG2ZTDheEAyKHWPIHnuJJBz\XOnbnPlJI9nKE[FQ2CgZpkheG:uYYLv[5JieGirYzDhcoFtgXOrcx?=	Moq5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{N{S5N\|koRjNyMke0PVM6RC:jPh?=
CCD-841-CoN	NV7JPVRuTnWwY4Tpc44h[XO\|YYm=	M3uyRlAvOSC2bzCxNEB2VQ>?	NGjp[IszKGi{cx?=	MlT1TY5lfWO2aX;uJI9nKEGPUFugdIhwe3Cqb4L5cIF1cW:wIHnuJIh2dWGwIFPDSE05PDFvQ3;OJINmdGy\|IHH0JFAvOSC2bzCxNEB2VSCjZoTldkAzKGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN?	NEmy[3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEK3OFk{QSd-M{CyO\|Q6Ozl:L3G+
CRC240	M17pcmFvfGm2dX3vdkBie3OjeR?=	MUK3NkBu\y:tZx?=		M{nkWGFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFPSR\|I1OCClZXzsd{B5\W6xZ4Lh[pRm\CCrbjDOU2QwW0OLRDDtc5V{\SCjdDC3NkBu\y:tZzygdI8h[WSvaX7pd5RmemWmIEGxJIRigXNiYX7kJI1m[XO3cnXkJI9vKGSjeTC0MEA5KGS3cnnu[{Bkd22yb4Xu[EBld3OrbnegZY5lKGSjeTCxNUBxd3O2IHzhd5Qh\G:\|ZR?=	M1\ybFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMke0PVM6Lz5\|MEK3OFk{QTxxYU6=
U2OS	MmTrSpVv[3Srb36gZZN{[Xl?		MWK2JIhzew>?	MUjJcoR2[3Srb36gc4YhT0[SLYTh[4dm\CCIenSxJEh2dmuwb4fuJI9zcWerbjmgbY51\XKwYXzpfoF1cW:wIHX4dJJme3OnZDDpckBpfW2jbjDVNm9UKGOnbHzzJIF1KDF{LkWgZYZ1\XJiNjDodpMh[nliY3;u[o9k[WxibXnjdo9{[2:yaXOgcYV1cG:m	M1zqUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMke0PVM6Lz5\|MEK3OFk{QTxxYU6=
A549	NXrZcXNQTnWwY4Tpc44h[XO\|YYm=		NGXKSIs3KGi{cx?=	NH3zR\|ZKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m\|IHnuJIh2dWGwIFG1OFkh[2WubIOgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5iY3HzdIF{\SB\|L{egZYN1cX[rdImgZYZ1\XJiNjDodpMh[nliY3HzdIF{\SCpbH:gN{84KGG\|c3H5	NYHHOoh4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CzO\|ExPjRpPkOwN\|cyODZ2PD;hQi=>
Caco2	NH;XVnZHfW6ldHnvckBie3OjeR?=		NFj5bmU3KGi{cx?=	MWXJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKEOjY3:yJINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGOjc4Dhd4UhOy95IHHjeIl3cXS7IHHmeIVzKDZiaILzJIJ6KGOjc4Dhd4Uh\2yxIEOvO{Bie3OjeR?=	NGWxdmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEO3NVA3PCd-M{CzO\|ExPjR:L3G+
AsPC1	M3nTRWZ2dmO2aX;uJIF{e2G7		MlP2OkBpenN?	Mn7PTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCDc2DDNUBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiClYYPwZZNmKDNxNzDhZ5Rqfmm2eTDh[pRmeiB4IHjyd{BjgSClYYPwZZNmKGeubzCzM\|ch[XO\|YYm=	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODN5MUC2OEc,OzB\|N{GwOlQ9N2F-
HEK293	NYfBblFMTnWwY4Tpc44h[XO\|YYm=		MlewPEBpenN?	NIHoeVJKdmirYnn0bY9vKG:oIGfueFNCNXO2aX31cIF1\WRiV370M4JmfGFvY3H0[Y5qdiCyYYToe4F6KGmwIFjFT\|I6OyClZXzsd{B1emGwc3\lZ5Rm\CC5aYToJJA5gFSRUF\sZZNpNCCUZX7pcIxiKGy3Y3nm[ZJie2VicHzhd41q\CCyUlytWGsh[W6mIIDMT28vOSCjZoTldkA5KGi{czDifUBFfWGuIITvdIZt[XOqIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3ME2wMlM1\|ryP	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODV3MUmwNUc,OzB3NUG5NFE9N2F-
LN229	NXLsXHR{SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NEDSeVQ2KGSjeYO=	MYLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEyQMkK5JINmdGy\|IHHmeIVzKDViZHH5d{BjgSCDbHHtZZJDdHWnIHHzd4F6NCCLQ{WwQVAvO87:TR?=	NWPXS3N2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C1PFMzPDhpPkOwOVg{OjR6PD;hQi=>
T98G	M4DMTmFvfGmycn;sbYZmemG2aY\lJIF{e2G7		NYPCPFVDPSCmYYnz	NHjwbnJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGS5PGch[2WubIOgZYZ1\XJiNTDkZZl{KGK7IFHsZY1iekKudXWgZZN{[XluIFnDOVA:OC5\|zszN	NU\ZZmRZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C1PFMzPDhpPkOwOVg{OjR6PD;hQi=>
U87	NVvxNnRtSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		Mk\IOUBl[Xm\|	NEK5W4VCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGW4O{Bk\WyuczDh[pRmeiB3IHThfZMh[nliQXzhcYFzSmy3ZTDhd5NigSxiSVO1NF0xNjQQvF2=	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODV6M{K0PEc,OzB3OEOyOFg9N2F-
U138MG	NIH3eVNCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		MUi1JIRigXN?	NUfDVZU6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDVNVM5VUdiY3XscJMh[W[2ZYKgOUBl[Xm\|IHL5JGFt[W2jclLseYUh[XO\|YYmsJGlEPTB;MD6z{txO	M3H1eVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyNUizNlQ5Lz5\|MEW4N\|I1QDxxYU6=
U373	MUPBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		M4jXTFUh\GG7cx?=	MoTxRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCXM{ezJINmdGy\|IHHmeIVzKDViZHH5d{BjgSCDbHHtZZJDdHWnIHHzd4F6NCCLQ{WwQVAvO87:TR?=	M3PSV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyNUizNlQ5Lz5\|MEW4N\|I1QDxxYU6=
HL60	NHTiN4FEgXSxdH;4bYNqfHliYYPzZZk>		MWGzJIRigXN?	M4CxdmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNPjBiY3XscJMhcW6ldXLheIVlKG[xcjCzJIRigXNiYomgR4VtdFSrdHXyMWdtdyCudX3pcoV{[2WwdDDhd5NigSxiSVO1NF0xNjQQvF2=	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTJ3M{WyPUc,OzF{NUO1Nlk9N2F-
KG1	NVvPNWZIS3m2b4TvfIlkcXS7IHHzd4F6		MmPON{Bl[Xm\|	NEjIW3JEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMTzFiY3XscJMhcW6ldXLheIVlKG[xcjCzJIRigXNiYomgR4VtdFSrdHXyMWdtdyCudX3pcoV{[2WwdDDhd5NigSxiSVO1NF0xNjN4zszN	NVXvZ5d7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{GyOVM2OjlpPkOxNlU{PTJ7PD;hQi=>
Jurkat	NIH5RohEgXSxdH;4bYNqfHliYYPzZZk>		NHTmO5M{KGSjeYO=	M3vyemN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGp2emujdDDj[YxteyCrbnP1ZoF1\WRiZn;yJFMh\GG7czDifUBE\WyuVHn0[ZIuT2yxIHz1cYlv\XOlZX70JIF{e2G7LDDJR\|UxRTBwNN88US=>	NUD3OnBvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{GyOVM2OjlpPkOxNlU{PTJ7PD;hQi=>
NALM6	Mo\UR5l1d3SxeHnjbZR6KGG\|c3H5		NXL5epdROyCmYYnz	NFvMXI9EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBPSUyPNjDj[YxteyCrbnP1ZoF1\WRiZn;yJFMh\GG7czDifUBE\WyuVHn0[ZIuT2yxIHz1cYlv\XOlZX70JIF{e2G7LDDJR\|UxRTBwNkNOwG0>	M3ztZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzMkWzOVI6Lz5\|MUK1N\|UzQTxxYU6=
HEK293	NVzMd3RNTnWwY4Tpc44h[XO\|YYm=		NUXXXXVWQTBibXnudy=>	M{f5TmlvcGmkaYTpc44hd2ZiS1nYMXJNfWOQIH\1d4VlKEOEUDCoeY5sdm:5bjDvdolocW5rIHLpcoRqdmdidH:gT2lFNVKOdXPDJIZ2e2WmIFPSSWIhMHWwa37ve44hd3KrZ3nuLUB1emGwc3\lZ5Rm\CCrbjDoeY1idiCKRVuyPVMh[2WubIOgdJJmcW6ldXLheIVlKHerdHigZ49ueG:3bnSgN\|AhdWmwczDi[YZwemViYnXmc5JmKG[xcoPrc4xqdiCjZHTpeIlwdiCjbnSgcYVie3W{ZXSgZYZ1\XJiOUCgcYlveyCrbjDwdoV{\W6lZTDv[kBkd2WuZX70[ZJigixiSVO1NF0yNjV\|zszN	NW\QR5dTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{GyOVM2OjlpPkOxNlU{PTJ7PD;hQi=>
293beta5	M1rMXGFvfGm4aYLhcEBie3OjeR?=		NUj4XI1ZPyCmYYnz	MknlRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTJVu[W5iYXTlco93cXK3czC1JIlv\mWldHXkJIlvKDJ7M3LleIE2KGOnbHzzJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[gdIxieXWnIH\vdo1ifGmxbjDpcoN2[mG2ZXSg[o9zKDdiZHH5d{BjgSCJRmCgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3ME2wMlbPxE1?	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OjB2NUKzPUc,OzJyNEWyN\|k9N2F-
A549	MX;BcpRqfmm{YXygZZN{[Xl?		MkDPOFghcHK\|	NXTvNlRiSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUHWvYX6gZYRmdm:4aYL1d{A2KGmwZnXjeIVlKGmwIHj1cYFvKEF3NEmgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC4aYLhcEBmdnS{eTDh[pRmeiB2ODDodpMh[nliR1\QJJJmeG:{dHXyJIdmdmViYnHz[YQh[XO\|YYmsJGlEPTB;MT6yNu69VQ>?	M1TQR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzN{MES1NlM6Lz5\|MkC0OVI{QTxxYU6=
A549	M4TNbWN6fG:2b4jpZ4l1gSCjc4PhfS=>		M4\rRlQ5KGi{cx?=	MX\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIF{e2W\|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZktKEOFNUC9NlIvQc7:TR?=	NIDaSmQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MkC0OVI{QSd-M{KwOFUzOzl:L3G+
Vero	NID3V\|dHfW6ldHnvckBie3OjeR?=			MULW[ZJwKGOnbHzzJJZq[WKrbHn0fUByUFSVIH\vdkBbcWujII\pdpV{KGmwaHnibZRwenN?	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OzJ{OUW0OUc,OzN{Mkm1OFU9N2F-
Vero	NH\TZm1CdnSrdnnyZYwh[XO\|YYm=		MU[yOEBpeg>?	NFzQe41CdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBUSVKVLVPvWk0zKCi4aYLhcEB1cXSncjmgcYVie3W{ZXSgZpkheGyjcYXlJIF{e2G7IHnuJHZmem9iY3XscJMh[XRiTV;JJFAvODF{NTDh[pRmeiB{NDDodkwhUUN3ME2wMlI5\|ryP	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxOFQ5Nyd-Q3jFUWJNRC:jPh?=
skeletal myoblast cells	M2jYR2N6fG:2b4jpZ4l1gSCjc4PhfS=>		NHPZPVk4OiCq	NInESllFVkSLOjDDfZRwfG:6aXPpeJkhcW5iVnn0do8tKDd{IHjveZItKGmwIILheEB{c2WuZYThcEBugW:kbHHzeEBk\WyuczygTWM2OD1{LkROwG0>	MnvTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ1QC9pPlPoSW1DVDxxYU6=
VERO-E6	NFTVXWRHfW6ldHnvckBie3OjeR?=		M3jFe\|Q5KGi{cx?=	M3PVOmRmfGW{bXnuZZRqd25ib3[gTWM2OCC4YXz1[ZMh\m:{IHnubIljcXSrb36gc4YhW0GUUz3Dc3YuOiCrbnT1Z4VlKGO7dH;0c5hq[2m2eTDv[kBXTVKRLVW2JINmdGy\|IHHmeIVzKDR6IHjveZJ{KCCneIDvd5Vz\SC2bzCwMlAyKE2RSTDTRXJUKEOxVj2yJJZqenW\|IHL5JIhq\2hiY3;ueIVvfCCrbXHnbY5oNCCLQ{WwQVQvOc7:TR?=	M2DxR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PDhxJ{7DbGVOSkx:L3G+

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	p-STAT3 / STAT3 / c-Myc / Survivin ; PubMed: 25970160 Prostate LNCaP, C4-2B or DU145 cells were treated with DMSO, 0.5 μM or 1 μM niclosamide overnight, cells were then stimulated with 10 ng/mL of IL6 for 30 minutes. Whole cell lysates were prepared and subjected to Western blot analysis using antibodies as indicated. p-BCR-ABL / BCR-ABL ; PubMed: 29358661 Cell Death Dis KBM5 cells harboring wild-type or T315I-BCR-ABL and K562 cell were exposed to different concentrations of niclosamide, and then analyzed by Western blotting analysis. p-STAT5 / STAT5 / p-AKT / AKT / p-ERK / ERK ; PubMed: 29358661 Cell Death Dis CML cells were treated with increasing concentrations of niclosamide for 48 h and subjected to Western blotting analysis. Actin served as a loading control for blots above, which were performed sequentially. β-catenin; PubMed: 27652012 Springerplus Niclosamide decreases intracellular β-catenin levels in A-498 and SW-839 cells. Cells were treated with niclosamide for 24 h prior to WB analysis.	25970160 29358661 27652012
Growth inhibition assay	Cell viability; PubMed: 28418862 Oncotarget Cell viability of colon cancer cells after treatment of niclosamide for a 48-h period. Cell viability was determined by trypan blue exclusion assay from triplicate experiments.	28418862

In vivo

Niclosamide(40 mg/kg/d, i.p.) inhibits growth of xenografted AML cells in nude mice bearing HL-60 xenograft tumors. ^[4]

Protocol

Kinase Assay: ^[1]	- Collapse Protein Kinase profiling assay: Assay for 22 different proteins kinases is carried out by ProQinase Gmbh. All of the protein kinases are expressed either in Sf9 insect cells or in E.coli as recombinant GST-fusion proteins or His-tagged proteins. Protein kinases are purified by affinity chromatography using either GSH-agarose or Ni_NTH-agarose. A radiometric protein kinase assay is used for measuring the kinase activity of the 22 protein kinases. Briefly, for each protein kinase, 50 μL reaction cocktail containing 60 mM HEPES-NaOH, 3 mM MgCl₂, 3 mM MnCl₂, 3 μM Na-orthovanadate, 1.2 mM DTT, 50 μg/mL PEG20000, 1 μM [γ-33P]-ATP, Niclosamide, adequate amount of enzyme and its substrate. The PKC-alpha assay additionally contain 1 mM Cacl₂, 4 mM EDTA, 5 μg/mL phosphatidylserine and 1 μg/mL 1, 2-Dioleyl-glycerol. The reaction cocktails are incubated at 37 °C for 60 minutes and stop with 50 μL 2% (v/v) H₃PO₄. Incorporation of 33Pi is determined with a microplate scintillation counter. The activities and the IC50 values are calculated using Quattro Workflow V2.28.
Cell Research: ^[1]	- Collapse Cell lines: Hela, A549, Du145, HT-29, A431, PC3 cells Concentrations: 16 μM Incubation Time: 72 hours Method: Cells are plated in 96-well culture plates with cell density of 3-4 × 10³ cells/well and treat with Niclosamide by adding 100 μL medium containing Niclosamide of various concentrations on the second day. After 72-hour's treatment, MTT is added to each well and incubated for additional 4-5 hours, and the absorbance is measured on a microplate reader at 570nm. Cell growth inhibition is evaluated as the ratio of the absorbance of the sample to that of the control. The results are representative of at least 3 independent experiments. (Only for Reference)
Animal Research: ^[4]	- Collapse Animal Models: nude mice bearing HL-60 xenograft tumors. Dosages: 40 mg/kg Administration: Intraperitoneal injection (Only for Reference)

References

[4] Jin Y, et al. Cancer Res, 2010, 70(6), 2516-2527.

- Collapse

Solubility (25°C)

In vitro	DMF	12 mg/mL warmed (36.68 mM)
	DMSO	Insoluble
	Water	Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Niclosamide SDF

Molecular Weight	327.12
Formula	C₁₃H₈Cl₂N₂O₄
CAS No.	50-65-7
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	Niclocide, BAY2353
Smiles	C1=CC(=C(C=C1[N+](=O)[O-])Cl)NC(=O)C2=C(C=CC(=C2)Cl)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)

% DMSO+ % + % Tween 80+ % ddH₂O

Calculate Reset

Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2021-01-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04436458	Not yet recruiting	Drug: Niclosamide Oral Tablet\|Drug: Placebo	COVID	First Wave Bio Inc.	December 2020	Phase 2
NCT04644705	Recruiting	Drug: Niclosamide\|Drug: Placebo	Healthy Volunteers	Charité Research Organisation GmbH\|Bayer	November 2 2020	Phase 1
NCT04592835	Not yet recruiting	Drug: DWRX2003	COVID-19 Patients	Daewoong Pharmaceutical Co. LTD.\|Novotech (Australia) Pty Limited	October 19 2020	Phase 1
NCT04524052	Not yet recruiting	Drug: DWRX2003\|Drug: Placebo	Healthy	Daewoong Pharmaceutical Co. LTD.	August 2020	Phase 1
NCT03521232	Recruiting	Drug: Niclosamide	Ulcerative Colitis\|Ulcerative Proctitis\|Ulcerative Proctosigmoiditis	First Wave Bio Inc.	May 15 2018	Phase 1\|Phase 2
NCT02687009	Terminated	Drug: Niclosamide	Colon Cancer	Michael Morse MD\|Duke University	November 7 2017	Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

STAT Signaling Pathway Map

Related STAT Products

Cerdulatinib (PRT062070) hydrochloride ^New

Cerdulatinib (PRT-062070, PRT2070) hydrochloride is an oral active, multi-targeted tyrosine kinase inhibitor with IC50 of 12 nM/6 nM/8 nM/0.5 nM and 32 nM for JAK1/JAK2/JAK3/TYK2 and Syk, respectively. Also inhibits 19 other tested kinases with IC50 less than 200 nM.
FLLL32 ^New

FLLL32 is a potent JAK2/STAT3 inhibitor with IC50 of <5 μM. FLLL32 inhibits the induction of STAT3 phosphorylation by IFNα and IL-6 in breast cancer cells.
S3I-201

S3I-201 (NSC 74859) shows potent inhibition of STAT3 DNA-binding activity with IC50 of 86 μM in cell-free assays, and low activity towards STAT1 and STAT5.

Features:A chemical probe inhibitor of Stat3 activity.
Fludarabine (NSC 118218)

Fludarabine (NSC 118218, FaraA, Fludarabinum) is a STAT1 activation inhibitor which causes a specific depletion of STAT1 protein (and mRNA) but not of other STATs. Also a DNA synthesis inhibitor in vascular smooth muscle cells. Fludarabine induces apoptosis.
Cryptotanshinone

Cryptotanshinone is a STAT3 inhibitor with IC50 of 4.6 μM in a cell-free assay, strongly inhibits phosphorylation of STAT3 Tyr705, with a small effect on STAT3 Ser727, but none against STAT1 nor STAT5. Cryptotanshinone induces ROS-dependent autophagy and mitochondria-mediated apoptosis.
Stattic

Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1. Stattic induces apoptosis.

Features:Stattic is the first non-peptide small molecule with inhibitory activity against STAT3 SH2 domain regardless of the STAT3 phosphorylation state in vitro.
Nifuroxazide

Nifuroxazide is a cell-permeable and orally available nitrofuran-based antidiarrheal agent that effectively suppresses the activation of cellular STAT1/3/5 transcription activity with IC50 of 3 μM against IL-6-induced STAT3 activation in U3A cells.
HO-3867

HO-3867, an analog of curcumin, is a selective STAT3 inhibitor that inhibits its phosphorylation, transcription, and DNA binding without affecting the expression of other active STATs. HO-3867 induces apoptosis.
SH-4-54

SH-4-54 is a potent STAT inhibitor with K_D of 300 nM and 464 nM for STAT3 and STAT5, respectively.

Choose Your Country or Region

By Signaling Pathways

By product type

Niclosamide