Niclosamide (BAY2353)

For research use only.

Catalog No.S3030 Synonyms: Niclocide, NSC 178296

28 publications

Niclosamide (BAY2353) Chemical Structure

CAS No. 50-65-7

Niclosamide (BAY2353, Niclocide, NSC 178296) can inhibit DNA replication and inhibit STAT3 with IC50 of 0.7 μM in a cell-free assay. Niclosamide selectively inhibited the phosphorylation of STAT3 and had no obvious inhibition against the activation of other homologues (e.g., STAT1 and STAT5).

Selleck's Niclosamide (BAY2353) has been cited by 28 publications

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Choose Selective STAT Inhibitors

Biological Activity

Description Niclosamide (BAY2353, Niclocide, NSC 178296) can inhibit DNA replication and inhibit STAT3 with IC50 of 0.7 μM in a cell-free assay. Niclosamide selectively inhibited the phosphorylation of STAT3 and had no obvious inhibition against the activation of other homologues (e.g., STAT1 and STAT5).
Targets
STAT3 [1]
(in Hela cells)
0.7 μM
In vitro

Niclosamide (< 5 μM) dose dependently inhibits STAT3-mediated luciferase reporter activity with IC50 of 0.25 μM in HeLa cells. Niclosamide(< 2 μM) dose dependently inhibits the phosphorylation of STAT3 in Du145 cells. Niclosamide (1 μM) inhibits the EGF-induced nuclear translocation of STAT3 in Du145 cells. Niclosamide(< 2 μM) dose dependently inhibits the transcription of STAT3 downstream genes in Du145 cells. Niclosamide(< 10 μM) dose dependently induces G0/G1 arrest and apoptosis of Du145 cancer cells. [1] Niclosamide is able to inhibit SARS-CoV replication at a micromolar concentration in Vero E6 cells infected with SARS-CoV. [2] Niclosamide(< 7.5 μM) promotes Frizzled1 endocytosis, downregulates Dishevelled-2 protein, and inhibits Wnt3A-stimulated beta-catenin stabilization and LEF/TCF reporter activity in U2OS cells. [3] Niclosamide inhibits the TNF-induced NF-κB reporter activity in a dose- and time-dependent manner in U2OS cells. Niclosamide(125 nM) inhibits NF-κB activation induced by p65, IKKα, IKKβ, IKKγ, and TAK1 in U2OS cells. Niclosamide(< 500 nM) completely block the time- and dose-dependent TNFα-induced alteration of the NF-κB–DNA complex in HL-60 cells. Niclosamide(< 10 nM) inhibits constitutive NF-κB activation in U266 cells. Niclosamide inhibits TNF-induced degradation of IκBα and relocation of p65 in a dose- and time-dependent manner in HL-60, Molm13, or AML primary cells. Niclosamide(500 nM) decreases TNF-induced NF-κB–dependent gene products involved in cell survival in HL-60 cells. Niclosamide dose dependently inhibits the growth and induces robust apoptosis of AML cells associated with decreased Mcl-1 and XIAP levels and increased intracellular ROS levels. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
PC3 Mmf3RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MYCxNlAhcHK| NFPzZ41CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGDDN{Bk\WyuczDh[pRmeiBzMkCgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1yLkVOwG0> MlrzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
A549 NV7zTllvSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M4LERVEzOCCqcoO= NX7mbIYxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEGyNEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUCuOO69VQ>? NW[4b|BjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[2PFAyPTlpPkG2OlgxOTV7PD;hQi=>
U87MG NWLOVYp5SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M3PDRlEzOCCqcoO= M3P5fGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iVUi3UWch[2WubIOgZYZ1\XJiMUKwJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD60{txO M{nKXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NkiwNVU6Lz5zNk[4NFE2QTxxYU6=
LoVo NHvyS3JCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NXvMOFJpOTJyIHjydy=> Mn\QRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCOb2\vJINmdGy|IHHmeIVzKDF{MDDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTBwN988US=> M{PUUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NkiwNVU6Lz5zNk[4NFE2QTxxYU6=
MIAPaCa2 M1[3[WFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MUSxNlAhcHK| MXrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2LQWDhR4Ez[2WubIOgZYZ1\XJiMUKwJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MT6x{txO MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjZ6MEG1PUc,OTZ4OECxOVk9N2F-
PC3 NF7ZSGNHfW6ldHnvckBie3OjeR?= NYX5TmF3OSCqch?= M1vVWWlvcGmkaYTpc44hd2ZibXn0c4Npd26mcnnhcEBu\W2kcnHu[UBxd3SnboTpZYwhcW5iaIXtZY4hWEN|IHPlcIx{KGGodHXyJFEhcHJ? MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjZ6MEG1PUc,OTZ4OECxOVk9N2F-
HEK293 NHfLSoRHfW6ldHnvckBie3OjeR?= MYqxJIhz MlXETY5pcWKrdHnvckBw\iCvaYTvZ4hwdmS{aXHsJI1mdWK{YX7lJJBwfGWwdHnhcEBqdiCqdX3hckBJTUt{OUOgZ4VtdHNiYX\0[ZIhOSCqch?= MkjtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
neural precursor MWXGeY5kfGmxbjDhd5NigQ>? NVXkSoI2UW6qaXLpeIlwdiCxZjDu[ZVzd3OyaHXy[UBxem:uaX\ldoF1cW:wIH;mJI1wfXOnIH7leZJidCCycnXjeZJ{d3JiY3XscJMh[nliTWTUJIF{e2G7 MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzRzN{[zNUc,OTd2MUe2N|E9N2F-
Vero E6 NYTOe2RZSW62aY\pdoFtKGG|c3H5 NF\ydVNCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBUSVKVIHPvdo9v[X[rcoXzJIlvKF[ncn:gSVYh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJidCC{ZYDsbYNifGmxbjDifUBGVEmVQTygSWM2ODxyLkJOwG0> MlX0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4NkO1N|koRjF5Nk[zOVM6RC:jPh?=
Vero E6 NXfPPHU5S3m2b4TvfIlkcXS7IHHzd4F6 M4rSNmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KF[ncn:gSVYh[2WubIOgZpkhVVSWIHHzd4F6NCCFQ{WwQVIzNjIQvF2= MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ4M{WzPUc,OTd4NkO1N|k9N2F-
RAW264.7 MWDDfZRweHKxdHXjeIl3\SCjc4PhfS=> NWXUSGJ1OTBidV2= MWLDfZRweHKxdHXjeIl3\SCjY4Tpeol1gSCjZ3HpcpN1KGGwdHjyZZghfG:6aX6gcIV1cGGuIH\hZ5Rwei:ycn;0[YN1cX[nIHHueIlo\W5vaX7keYNm\CClZXzsJIRm[XSqIHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGG2IEGwJJVOKGK7IF3UWEBz\WS3Y4Tpc44h[XO|YYm= NHT2Oog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUW0NFc3PCd-MUm1OFA4PjR:L3G+
CHO NF\nVohEgXSxcILveIVkfGm4ZTDhd5NigQ>? NUiwUmhrS3m2b4Dyc5Rm[3SrdnWgZYN1cX[rdImgZYdicW6|dDDhcpRpemG6IH\1d4lwdiC2b4jpckBHWDV7LXnu[JVk\WRiY3XscEBl\WG2aDDpckBEUE9iY3XscJMh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[nliTWTUJJJm\HWldHnvckBie3OjeR?= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTV2MEe2OEc,OTl3NEC3OlQ9N2F-
CHO M2XXd2Z2dmO2aX;uJIF{e2G7 MoHrTY5pcWKrdHnvckBw\iCEYXPpcIx2eyCjboTodoFkcXNiYX70bJJigCCycn;0[YN1cX[nIHHueIlo\W5iaHXweIFu\XJicILlMZBwemVidH:gdI9z\SClb372[ZJ{cW:wIHnuJGNOTzJvZYjwdoV{e2mwZzDDTG8h[2WubIO= NWTEc|JHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1OFA4PjRpPkG5OVQxPzZ2PD;hQi=>
Ava5 MUjBcpRqfmm{YXygZZN{[Xl? M4\RVlMh\GG7cx?= NGHYdXJCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJS1ZiZ3Xuc5R6eGViMXKgbY5n\WO2ZXSgbY4hSX[jNTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJJJmeGyrY3H0bY9vKGGodHXyJFMh\GG7czDifUBjdG:2IHj5ZpJq\Gm8YYTpc44h[W6jbInzbZMtKEWFNUC9NE4yPs7:TR?= NXfIR|dCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwOVk6QDNpPkKyNFU6QTh|PD;hQi=>
Ava5 NXHDVY1jSW62aY\pdoFtKGG|c3H5 NFrEUHE{KGSjeYO= NIn3bWFCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJS1ZiZ3Xuc5R6eGViMXKgbY5n\WO2ZXSgbY4hSX[jNTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJJJmeGyrY3H0bY9vKGGodHXyJFMh\GG7czDifUBjdG:2IHj5ZpJq\Gm8YYTpc44h[W6jbInzbZMtKEWFOUC9NE44|ryP NELGclI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC1PVk5Oyd-MkKwOVk6QDN:L3G+
Ava5 NFfSSo9EgXSxdH;4bYNqfHliYYPzZZk> M176[VMh\GG7cx?= NVrybodmS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSX[jNTDj[YxteyCjZoTldkA{KGSjeYOgZpkhdmW3dILhcEBz\WRiZInlJIF{e2G7LDDDR|UxRTFyzszN NXfue5k1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwOVk6QDNpPkKyNFU6QTh|PD;hQi=>
HFF MYnBcpRq[XCrY3;tdIxmgGGwIHHzd4F6 Mn\aNlQhcHK| M3\I[2FvfGmjcHnjc41xdGW6YX6gZYN1cX[rdImgZYdicW6|dDDUc5hweGyjc33hJIdwdmSraTDSTEB1[WOqeYrvbZRmeyCrbn\lZ5Rm\CCrbjDISmYh[2WubIOgZZN{\XO|ZXSgZZMhYzOKXT31doFkcWxiaX7jc5Jxd3KjdHnvckBi\nSncjCyOEBpenNiYomgd4NqdnSrbHzheIlwdiCudX3pcoV{[2WwY3WgZ492dnSncjygUWlEQTB;MD6y{txO MmjNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7N{C5N|coRjJ{OUewPVM4RC:jPh?=
HFF MUPBcpRq[XCrY3;tdIxmgGGwIHHzd4F6 NYn1eVAyOjRiaILz NHHYbnRCdnSrYYDpZ49ueGyneHHuJIFkfGm4aYT5JIFo[Wmwc4SgWI95d3CuYYPtZUBod26maXmgVmghfGGlaIn6c4l1\XNiaX7m[YN1\WRiaX6gTGZHKGOnbHzzJIF{e2W|c3XkJIF{KFt|SG2teZJi[2muIHnuZ49zeG:{YYTpc44h[W[2ZYKgNlQhcHK|IHL5JJNkcW62aXzsZZRqd25ibIXtbY5me2OnbnPlJINwfW62ZYKsJG1KSzVyPUCuNu69VQ>? MnfhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7N{C5N|coRjJ{OUewPVM4RC:jPh?=
MDA-MB-231 MoPRR5l1d3SxeHnjbZR6KGG|c3H5 MljzR3l1d3SxeHnjbZR6KGGpYXnud5QhTVJvbnXnZZRqfmViaIXtZY4hVUSDLV3CMVI{OSClZXzsd{BjgSCPVGOgZZN{[XluIFnDOVA:OC55Od88US=> M2LvS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEG2NVkyLz5{M{SxOlE6OTxxYU6=
MCF7 Mni1R5l1d3SxeHnjbZR6KGG|c3H5 MkTYR3l1d3SxeHnjbZR6KGGpYXnud5QhTVJvcH;zbZRqfmViaIXtZY4hVUOINzDj[YxteyCkeTDNWHMh[XO|YYmsJGlEPTB;MT6wOu69VQ>? NYnBU4I3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NVYyQTFpPkKzOFE3OTlzPD;hQi=>
AsPC1 MUPDfZRwfG:6aXPpeJkh[XO|YYm= MnPFR3l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRZNRSzFiY3XscJMh[nliTWTTJIF{e2G7LDDJR|UxRTFwNEhOwG0> M{\FbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEG2NVkyLz5{M{SxOlE6OTxxYU6=
PANC1 MXPDfZRwfG:6aXPpeJkh[XO|YYm= Mm\uR3l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVGFPSzFiY3XscJMh[nliTWTTJIF{e2G7LDDJR|UxRTFwN{ROwG0> M1\WXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEG2NVkyLz5{M{SxOlE6OTxxYU6=
HEK293 Mkn5SpVv[3Srb36gZZN{[Xl? NYjrd2pKQCCqcoO= MXTJcohq[mm2aX;uJI9nKFewdEPBM4JmfGFvY3Hz[YlvKHOrZ37hcIlv\yCrbjDISWszQTNiY3XscJMh[W[2ZYKgPEBpenNiYomgWG9R\myjc3igdoVxd3K2ZYKgZZN{[XluIFnDOVA:OC52zszN M4m1bFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEWzNFc{Lz5{M{S1N|A4OzxxYU6=
U2OS NEKwVpNHfW6ldHnvckBie3OjeR?= NHXYOVMyOi53IIXN MV62JIhzew>? MWLJcoR2[3Srb36gc4YhcW62ZYLuZYxqgmG2aX;uJI9nKE[{aYr6cIVlOS2JRmCgLJVvc26xd36gc5Jq\2mwKTDlfJBz\XO|ZXSgbY4hcHWvYX6gWVJQWyClZXzsd{BifCBzMj61JJVOKGGodHXyJFYhcHK|IHL5JINwdm[xY3HsJI1q[3Kxc3PvdJk> MkTTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NUOwO|MoRjJ|NEWzNFc{RC:jPh?=
MDA-MB-231 MoTzR5l1d3SxeHnjbZR6KGG|c3H5 MWC3NkBpenN? MY\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDFVkBv\WejdHn2[UBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHMh[XO|YYmsJGlEPTB;MD63Pe69VQ>? NUPaNVl6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVk3OTNpPkKzOFU6PjF|PD;hQi=>
MCF7 MWPDfZRwfG:6aXPpeJkh[XO|YYm= MWW3NkBpenN? MYTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDFVkBxd3OrdHn2[UBOS0Z5IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFNiYYPzZZktKEmFNUC9NU4xPs7:TR?= NF3Od4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S1PVYyOyd-MkO0OVk3OTN:L3G+
AsPC1 NHrQfY9EgXSxdH;4bYNqfHliYYPzZZk> NIPZcnQ4OiCqcoO= NW\SfG5sS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSXOSQ{GgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WUzDhd5NigSxiSVO1NF0yNjR5zszN NU\xWGI2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVk3OTNpPkKzOFU6PjF|PD;hQi=>
PANC1 NILuOFhEgXSxdH;4bYNqfHliYYPzZZk> M2DPTFczKGi{cx?= M1nqcWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHBCVkNzIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFNiYYPzZZktKEmFNUC9NU44O87:TR?= MorpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NUm2NVMoRjJ|NEW5OlE{RC:jPh?=
MDA-MB-231 MX7BcpRqfHWvb4KgZZN{[Xl? MnLaO|UhdWdxa3e= NV\VeItDPSCmYYnz NFvvcYpCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDFVkBv\WejdHn2[UBOTEFvTVKtNlMyKGOnbHzzJJhmdm:pcnHmeIVlKGmwIH71[IUhdW:3c3WgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD0eY1weiCpcn;3eIgh[XRiN{WgcYcwc2duIIDvJJFlKG[xcjC1JIRigXN? NILs[JY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S1PVYyOyd-MkO0OVk3OTN:L3G+
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MDA-MB-231 NX7BOmxlSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MVyxNEB1dyB{MDD1US=> NHrGWZYzPCCqcoO= NWLWfHV4SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDFVkBv\WejdHn2[UBOTEFvTVKtNlMyKGOnbHzzJIF1KDFyIITvJFIxKHWPIHHmeIVzKDJ2IHjyd{BjgSCPVGSgZZN{[Xl? M3e3dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEW5OlE{Lz5{M{S1PVYyOzxxYU6=
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MDA-MB-231 M1;qR2Z2dmO2aX;uJIF{e2G7 NWrxfFBHOSC2bzCxNEB2VQ>? MUW0PEBpenN? NH70b|FKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJIh2dWGwIFXSJI5m\2G2aY\lJG1FSS2PQj2yN|Eh[2WubIOgZZQhOSC2bzCxNEB2VSCjZoTldkA1QCCqcoOgZpkhdGmpaISgcYlkem:|Y3;wbYMh[W6jbInzbZM> NVrmXG04RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVk3OTNpPkKzOFU6PjF|PD;hQi=>
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DU145 M4jyUGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MXu3NkBpenN? M{j5emFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iRGWxOFUh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1yLkhOwG0> NETVUXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFI{OSd-MkS5NFAzOzF:L3G+
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HT-29 NWDvd|FMSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NV;WXIZUPzJiaILz NH;FWXNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjUMVI6KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;Nz6y{txO NUDUUVBuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFAzOzFpPkK0PVAxOjNzPD;hQi=>
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HCT116 NYmzNY9mTnWwY4Tpc44h[XO|YYm= NGTTcGI2KHWP M4C2T|E5KGi{cx?= NGjWOG1KdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5ic3nncoFtcW6pIHnuJIh2dWGwIFjDWFEyPiClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iCFeXPsbY4hTDFicILveIVqdiCneIDy[ZN{cW:wIHH0JFUhfU1iYX\0[ZIhOThiaILzJIJ6KFenc4Tldo4h[myxdDDt[ZRpd2R? NYHnW|VGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiyN|M3QDBpPkK4NlM{PjhyPD;hQi=>
HCT116 MnHBSpVv[3Srb36gZZN{[Xl? M335SlUhfU1? NUTGZWF1OThiaILz MU\Jcohq[mm2aX;uJI9nKFewdD;i[ZRiNWOjdHXubY4he2mpbnHsbY5oKGmwIHj1cYFvKEiFVEGxOkBk\WyuczDhd5Nme3OnZDDhd{Bld3ewcnXneYxifGmxbjDv[kB{fXK4aY\pckBxem:2ZXnuJIV5eHKnc4Ppc44h[XRiNTD1UUBi\nSncjCxPEBpenNiYomgW4V{fGW{bjDicI91KG2ndHjv[C=> NIOwPGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEKzN|Y5OCd-MkiyN|M3QDB:L3G+
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BT-37 MXPxTHRUKGG|c3H5 NH\FcnFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQmStN|ch[2WubIO= NFu2NZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
NB-EBc1 Ml\2dWhVWyCjc4PhfS=> NV3TUZFweUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF7CMWVD[zFiY3XscJM> NX3YVoFZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Saos-2 NHvUdIFyUFSVIHHzd4F6 NHPsV4NyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU3Hvd{0zKGOnbHzz NEPhbZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-SH M3nrUJFJXFNiYYPzZZk> NH30[|ZyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1UUCClZXzsdy=> NFfQR4w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
NB1643 MXLxTHRUKGG|c3H5 M4rHNpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCQQkG2OFMh[2WubIO= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
LAN-5 MlOzdWhVWyCjc4PhfS=> MXPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVEGQLUWgZ4VtdHN? MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
BT-12 MnyxdWhVWyCjc4PhfS=> NHnyZZVyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQmStNVIh[2WubIO= MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh18 MU\xTHRUKGG|c3H5 MXfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmhzODDj[Yxtew>? MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
OHS-50 MV3xTHRUKGG|c3H5 MVnxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy| MlzSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
RD MWrxTHRUKGG|c3H5 NUe3c2F6eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLEJINmdGy| MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
MG 63 (6-TG R) NF7jS4lyUFSVIHHzd4F6 NHPQ[GJyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTVegOlMhMDZvVFegVkkh[2WubIO= M17vU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh41 MWTxTHRUKGG|c3H5 MV7xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmh2MTDj[Yxtew>? MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
NB1643 M1\sbJFJXFNiYYPzZZk> NUHqN2RmeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhVkJzNkSzJINmdGy| M4\FTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
A673 M1rUSpFJXFNiYYPzZZk> NUDIS3A3eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhSTZ5MzDj[Yxteyl? MojhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC NW[4NY5xeUiWUzDhd5NigQ>? MXHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTT{1PNU2FIHPlcIx{ MoPmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
BT-12 M1;iUZFJXFNiYYPzZZk> NFTadGdyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBDXC1zMjDj[Yxtew>? MmGyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
LAN-5 NXLhSGtIeUiWUzDhd5NigQ>? Mk\2dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUGFPNTViY3XscJM> MmXaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
DAOY M3Hpe5FJXFNiYYPzZZk> M4LRbpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJGRCV1liY3XscJM> NIfaWmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
NB-EBc1 MnGwdWhVWyCjc4PhfS=> NWH5dnFIeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhVkJvRVLjNUBk\Wyucx?= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SJ-GBM2 NHywT3ZyUFSVIHHzd4F6 NEHuWW1yUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBUUi2JQl2yJINmdGy| M1nxTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
BT-37 Mm\PdWhVWyCjc4PhfS=> MmnmdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgRnQuOzdiY3XscJM> NFrZU2E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
TC32 MXrxTHRUKGG|c3H5 NWPVUIRHeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhXEN|MjDj[Yxtew>? MkPwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
MG 63 (6-TG R) MmPGdWhVWyCjc4PhfS=> M{jCV5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJG1IKDZ|IDi2MXRIKFJrIHPlcIx{ NWPURoRbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
fibroblast cells M3u3U5FJXFNiYYPzZZk> NWH0WXVKeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5Ih[2:wdILvcEBJcCC5aXzkJJR6eGViZnnido9jdGG|dDDj[Yxtew>? MkPzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
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Rh41 NHXiTnZyUFSVIHHzd4F6 MmHhdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgVog1OSClZXzsdy=> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
RD M2rmTJFJXFNiYYPzZZk> NFzuSZNyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBTTCClZXzsdy=> NFPMS4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
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Rh30 M3PSZ5FJXFNiYYPzZZk> MnHvdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgVog{OCClZXzsdy=> NV7aPIdURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Saos-2 MVjxTHRUKGG|c3H5 MUHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTZY9{NTJiY3XscJM> NFm0dW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
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LN229 MVTBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M322dVUh\GG7cx?= NGrP[oRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFzONlI6KGOnbHzzJIFnfGW{IEWg[IF6eyCkeTDBcIFu[XKEbIXlJIF{e2G7LDDJR|UxRTBwM988US=> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODV6M{K0PEc,OzB3OEOyOFg9N2F-
T98G NU\p[Io6SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= Mn7vOUBl[Xm| NVf3e5hkSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDUPVhIKGOnbHzzJIFnfGW{IEWg[IF6eyCkeTDBcIFu[XKEbIXlJIF{e2G7LDDJR|UxRTBwM988US=> MlzJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB3OEOyOFgoRjNyNUizNlQ5RC:jPh?=
U87 MXHBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M{XrcFUh\GG7cx?= MVzBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFV6NzDj[YxteyCjZoTldkA2KGSjeYOgZpkhSWyjbXHyRox2\SCjc4PhfUwhUUN3ME2wMlPPxE1? NHX4Xmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEW4N|I1QCd-M{C1PFMzPDh:L3G+
U138MG Mn;kRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MYO1JIRigXN? Mo\WRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCXMUO4UWch[2WubIOgZYZ1\XJiNTDkZZl{KGK7IFHsZY1iekKudXWgZZN{[XluIFnDOVA:OC5|zszN NXq4cJQ3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C1PFMzPDhpPkOwOVg{OjR6PD;hQi=>
U373 NYPJR2UySW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MUi1JIRigXN? MXTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFV|N{OgZ4VtdHNiYX\0[ZIhPSCmYYnzJIJ6KEGuYX3hdmJtfWViYYPzZZktKEmFNUC9NE4{|ryP MkLJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB3OEOyOFgoRjNyNUizNlQ5RC:jPh?=
HL60 MXXDfZRwfG:6aXPpeJkh[XO|YYm= NISzNos{KGSjeYO= M3LzPGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNPjBiY3XscJMhcW6ldXLheIVlKG[xcjCzJIRigXNiYomgR4VtdFSrdHXyMWdtdyCudX3pcoV{[2WwdDDhd5NigSxiSVO1NF0xNjQQvF2= NVXofpl6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{GyOVM2OjlpPkOxNlU{PTJ7PD;hQi=>
KG1 NFHHdGpEgXSxdH;4bYNqfHliYYPzZZk> MmS5N{Bl[Xm| MXLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLS|Eh[2WubIOgbY5kfWKjdHXkJIZweiB|IHThfZMh[nliQ3XscHRqfGW{LVfsc{BtfW2rbnXzZ4VvfCCjc4PhfUwhUUN3ME2wMlM3|ryP MnPEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF{NUO1NlkoRjNzMkWzOVI6RC:jPh?=
Jurkat M1jXcmN6fG:2b4jpZ4l1gSCjc4PhfS=> MVuzJIRigXN? Mm\QR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTpVzc2G2IHPlcIx{KGmwY4XiZZRm\CCob4KgN{Bl[Xm|IHL5JGNmdGyWaYTldk1IdG9ibIXtbY5me2OnboSgZZN{[XluIFnDOVA:OC52zszN NFzUc4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUK1N|UzQSd-M{GyOVM2Ojl:L3G+
NALM6 MUnDfZRwfG:6aXPpeJkh[XO|YYm= M3;uWlMh\GG7cx?= NGDFXnpEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBPSUyPNjDj[YxteyCrbnP1ZoF1\WRiZn;yJFMh\GG7czDifUBE\WyuVHn0[ZIuT2yxIHz1cYlv\XOlZX70JIF{e2G7LDDJR|UxRTBwNkNOwG0> NXvwfm5bRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{GyOVM2OjlpPkOxNlU{PTJ7PD;hQi=>
HEK293 NXniWmdyTnWwY4Tpc44h[XO|YYm= NF6zOGg6OCCvaX7z M1vQcmlvcGmkaYTpc44hd2ZiS1nYMXJNfWOQIH\1d4VlKEOEUDCoeY5sdm:5bjDvdolocW5rIHLpcoRqdmdidH:gT2lFNVKOdXPDJIZ2e2WmIFPSSWIhMHWwa37ve44hd3KrZ3nuLUB1emGwc3\lZ5Rm\CCrbjDoeY1idiCKRVuyPVMh[2WubIOgdJJmcW6ldXLheIVlKHerdHigZ49ueG:3bnSgN|AhdWmwczDi[YZwemViYnXmc5JmKG[xcoPrc4xqdiCjZHTpeIlwdiCjbnSgcYVie3W{ZXSgZYZ1\XJiOUCgcYlveyCrbjDwdoV{\W6lZTDv[kBkd2WuZX70[ZJigixiSVO1NF0yNjV|zszN Mn72QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF{NUO1NlkoRjNzMkWzOVI6RC:jPh?=
293beta5 M1fF[GFvfGm4aYLhcEBie3OjeR?= MmDnO{Bl[Xm| MonYRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTJVu[W5iYXTlco93cXK3czC1JIlv\mWldHXkJIlvKDJ7M3LleIE2KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gdIxieXWnIH\vdo1ifGmxbjDpcoN2[mG2ZXSg[o9zKDdiZHH5d{BjgSCJRmCgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3ME2wMlbPxE1? NHPRWYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MkC0OVI{QSd-M{KwOFUzOzl:L3G+
A549 M2XKe2FvfGm4aYLhcEBie3OjeR?= MUW0PEBpenN? NEP6SGhCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJfW2jbjDh[IVvd3[rcoXzJFUhcW6oZXP0[YQhcW5iaIXtZY4hSTV2OTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJIVvfHK7IHHmeIVzKDR6IHjyd{BjgSCJRmCgdoVxd3K2ZYKg[4Vv\SCkYYPl[EBie3OjeTygTWM2OD1zLkKy{txO NVfRNmk1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{KwOFUzOzlpPkOyNFQ2OjN7PD;hQi=>
A549 M1LE[mN6fG:2b4jpZ4l1gSCjc4PhfS=> MWG0PEBpenN? NFjGZ5VEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliYXzhcYFzKGKudXWgZZN{[XluIFPDOVA:OjJwOd88US=> NVTBdnpSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{KwOFUzOzlpPkOyNFQ2OjN7PD;hQi=>
Vero NVjJenVPTnWwY4Tpc44h[XO|YYm= NGGwTmZX\XKxIHPlcIx{KH[rYXLpcIl1gSCzSGTTJIZweiCcaXvhJJZqenW|IHnubIljcXSxcoO= M3;CTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzN|MkK5OVQ2Lz5|M{KyPVU1PTxxYU6=
Vero M37NRWFvfGm4aYLhcEBie3OjeR?= MV:yOEBpeg>? M1LXbGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JHNCWlNvQ3;WMVIhMH[rcnHsJJRqfGW{KTDt[YF{fXKnZDDifUBxdGGzdXWgZZN{[XliaX6gWoVzdyClZXzsd{BifCCPT1mgNE4xOTJ3IHHmeIVzKDJ2IHjyMEBKSzVyPUCuNljPxE1? Mo[zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ1QC9pPlPoSW1DVDxxYU6=
skeletal myoblast cells MY\DfZRwfG:6aXPpeJkh[XO|YYm= NUGyfYdmPzJiaB?= MlS2SG5FUTpiQ4n0c5RwgGmlaYT5JIlvKF[rdILvMEA4OiCqb4XyMEBqdiC{YYSgd4tmdGW2YXygcZlw[myjc4SgZ4VtdHNuIFnDOVA:Oi5|zszN M3ewV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PDhxJ{7DbGVOSkx:L3G+
VERO-E6 NILZbW9HfW6ldHnvckBie3OjeR?= MkT4OFghcHK| MXXE[ZRmem2rbnH0bY9vKG:oIFnDOVAhfmGudXXzJIZweiCrbnjpZol1cW:wIH;mJHNCWlNvQ3;WMVIhcW6mdXPl[EBkgXSxdH;4bYNqfHlib3[gWmVTVy2HNjDj[YxteyCjZoTldkA1QCCqb4Xyd{Ah\Xiyb4P1doUhfG9iMD6wNUBOV0liU1HSV{BEd1ZvMjD2bZJ2eyCkeTDobYdpKGOxboTlcpQhcW2jZ3nu[{whUUN3ME20MlHPxE1? MoG5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ1QC9pPlPoSW1DVDxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-STAT3 / STAT3 / c-Myc / Survivin ; 

PubMed: 25970160     


LNCaP, C4-2B or DU145 cells were treated with DMSO, 0.5 μM or 1 μM niclosamide overnight, cells were then stimulated with 10 ng/mL of IL6 for 30 minutes. Whole cell lysates were prepared and subjected to Western blot analysis using antibodies as indicated.

p-BCR-ABL / BCR-ABL ; 

PubMed: 29358661     


KBM5 cells harboring wild-type or T315I-BCR-ABL and K562 cell were exposed to different concentrations of niclosamide, and then analyzed by Western blotting analysis.

p-STAT5 / STAT5 / p-AKT / AKT / p-ERK / ERK ; 

PubMed: 29358661     


CML cells were treated with increasing concentrations of niclosamide for 48 h and subjected to Western blotting analysis. Actin served as a loading control for blots above, which were performed sequentially.

β-catenin; 

PubMed: 27652012     


Niclosamide decreases intracellular β-catenin levels in A-498 and SW-839 cells. Cells were treated with niclosamide for 24 h prior to WB analysis.

25970160 29358661 27652012
Growth inhibition assay
Cell viability; 

PubMed: 28418862     


Cell viability of colon cancer cells after treatment of niclosamide for a 48-h period. Cell viability was determined by trypan blue exclusion assay from triplicate experiments.

28418862
In vivo Niclosamide(40 mg/kg/d, i.p.) inhibits growth of xenografted AML cells in nude mice bearing HL-60 xenograft tumors. [4]

Protocol

Kinase Assay:

[1]

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Protein Kinase profiling assay:

Assay for 22 different proteins kinases is carried out by ProQinase Gmbh. All of the protein kinases are expressed either in Sf9 insect cells or in E.coli as recombinant GST-fusion proteins or His-tagged proteins. Protein kinases are purified by affinity chromatography using either GSH-agarose or Ni_NTH-agarose. A radiometric protein kinase assay is used for measuring the kinase activity of the 22 protein kinases. Briefly, for each protein kinase, 50 μL reaction cocktail containing 60 mM HEPES-NaOH, 3 mM MgCl2, 3 mM MnCl2, 3 μM Na-orthovanadate, 1.2 mM DTT, 50 μg/mL PEG20000, 1 μM [γ-33P]-ATP, Niclosamide, adequate amount of enzyme and its substrate. The PKC-alpha assay additionally contain 1 mM Cacl2, 4 mM EDTA, 5 μg/mL phosphatidylserine and 1 μg/mL 1, 2-Dioleyl-glycerol. The reaction cocktails are incubated at 37 °C for 60 minutes and stop with 50 μL 2% (v/v) H3PO4. Incorporation of 33Pi is determined with a microplate scintillation counter. The activities and the IC50 values are calculated using Quattro Workflow V2.28.
Cell Research:

[1]

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  • Cell lines: Hela, A549, Du145, HT-29, A431, PC3 cells
  • Concentrations: 16 μM
  • Incubation Time: 72 hours
  • Method:

    Cells are plated in 96-well culture plates with cell density of 3-4 × 103 cells/well and treat with Niclosamide by adding 100 μL medium containing Niclosamide of various concentrations on the second day. After 72-hour's treatment, MTT is added to each well and incubated for additional 4-5 hours, and the absorbance is measured on a microplate reader at 570nm. Cell growth inhibition is evaluated as the ratio of the absorbance of the sample to that of the control. The results are representative of at least 3 independent experiments.


    (Only for Reference)
Animal Research:

[4]

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  • Animal Models: nude mice bearing HL-60 xenograft tumors.
  • Dosages: 40 mg/kg
  • Administration: Intraperitoneal injection
    (Only for Reference)

Solubility (25°C)

In vitro DMF 12 mg/mL warmed (36.68 mM)
DMSO Insoluble
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 327.12
Formula

C13H8Cl2N2O4

CAS No. 50-65-7
Storage powder
in solvent
Synonyms Niclocide, NSC 178296
Smiles C1=CC(=C(C=C1[N+](=O)[O-])Cl)NC(=O)C2=C(C=CC(=C2)Cl)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Step 2: Enter the in vivo formulation ()
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04436458 Not yet recruiting Drug: Niclosamide Oral Tablet|Drug: Placebo COVID First Wave Bio Inc. June 2021 Phase 2
NCT04644705 Recruiting Drug: Niclosamide|Drug: Placebo Healthy Volunteers Charité Research Organisation GmbH|Bayer November 2 2020 Phase 1
NCT04592835 Not yet recruiting Drug: DWRX2003 COVID-19 Patients Daewoong Pharmaceutical Co. LTD.|Novotech (Australia) Pty Limited October 19 2020 Phase 1
NCT04524052 Not yet recruiting Drug: DWRX2003|Drug: Placebo Healthy Daewoong Pharmaceutical Co. LTD. August 2020 Phase 1
NCT03521232 Recruiting Drug: Niclosamide Ulcerative Colitis|Ulcerative Proctitis|Ulcerative Proctosigmoiditis First Wave Bio Inc. May 15 2018 Phase 1|Phase 2
NCT02687009 Terminated Drug: Niclosamide Colon Cancer Michael Morse MD|Duke University November 7 2017 Phase 1

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID