Niclosamide (BAY2353)

Name: Niclosamide (BAY2353)
Brand: Selleck Chemicals
SKU: S3030
Rating: 5 (28 reviews)

For research use only.

Catalog No.S3030 Synonyms: Niclocide, NSC 178296

28 publications

Niclosamide (BAY2353) Chemical Structure

CAS No. 50-65-7

Niclosamide (BAY2353, Niclocide, NSC 178296) can inhibit DNA replication and inhibit STAT3 with IC50 of 0.7 μM in a cell-free assay. Niclosamide selectively inhibited the phosphorylation of STAT3 and had no obvious inhibition against the activation of other homologues (e.g., STAT1 and STAT5).

Selleck's Niclosamide (BAY2353) has been cited by 28 publications

Cell Death Differ, 2021, 10.1038/s41418-021-00781-4

PubMed: 33963314 ( click the link to review the publication )

Biochim Biophys Acta Mol Cell Res, 2021, 1868(10):119080

PubMed: 34174290 ( click the link to review the publication )

Cell Death Dis, 2020, 11(12):1035

PubMed: 33279931 ( click the link to review the publication )

Cell Death Dis, 2020, 11(6):466

PubMed: 32546700 ( click the link to review the publication )

J Cell Mol Med, 2020, 5

PubMed: 32372557 ( click the link to review the publication )

Biomed J, 2020, S2319-4170(20)30066-4

PubMed: 32563698 ( click the link to review the publication )

Clin Exp Pharmacol Physiol, 2020, 4

PubMed: 32248559 ( click the link to review the publication )

bioRxiv, 2020, 2020/9/20.4.7.30734

PubMed: 32511305 ( click the link to review the publication )

J Invest Dermatol, 2020, S0022-202X(20)31981-3

PubMed: 32805217 ( click the link to review the publication )

Nat Microbiol, 2019, 4(5):813-825

PubMed: 30833724 ( click the link to review the publication )

J Immunother Cancer, 2019, 7(1):245

PubMed: 31511071 ( click the link to review the publication )

Mol Immunol, 2019, 114:369-377

PubMed: 31450182 ( click the link to review the publication )

Fish Shellfish Immunol, 2019, 92:813-820

PubMed: 31271840 ( click the link to review the publication )

Oncol Rep, 2019, 41(4):2389-2395

PubMed: 30816524 ( click the link to review the publication )

Thorac Cancer, 2019, 10(4):848-855

PubMed: 30810286 ( click the link to review the publication )

Eur Rev Med Pharmacol Sci, 2019, 23(3):1055-1062

PubMed: 30779072 ( click the link to review the publication )

Exp Ther Med, 2019, 18(5):4022-4030

PubMed: 31611940 ( click the link to review the publication )

J Clin Endocrinol Metab, 2018, 103(10):3706-3713

PubMed: 30053001 ( click the link to review the publication )

Oncol Rep, 2018, 40(6):3743-3751

PubMed: 30272302 ( click the link to review the publication )

Autoimmunity, 2018, 51(4):191-198

PubMed: 29869537 ( click the link to review the publication )

Acta Pharm Sin B, 2018, 8(6):889-899

PubMed: 30505658 ( click the link to review the publication )

Neoplasia, 2018, 20(10):1008-1022

PubMed: 30189359 ( click the link to review the publication )

Oncol Lett, 2018, 15(4):5772-5780

PubMed: 29545902 ( click the link to review the publication )

Oncotarget, 2018, 9(10):9273-9284

PubMed: 29507689 ( click the link to review the publication )
Onco Targets Ther, 2017, 10:1767-1776

PubMed: 28367059 ( click the link to review the publication )

Oncotarget, 2017, 8(33):54265-54276

PubMed: 28903339 ( click the link to review the publication )

Int Immunopharmacol, 2016, 36:199-204

PubMed: 27160867 ( click the link to review the publication )

Neoplasia, 2015, 17(7):586-97

PubMed: 26297436 ( click the link to review the publication )

Purity & Quality Control

Choose Selective STAT Inhibitors

Biological Activity

Description

Targets

STAT3 ^[1]
(in Hela cells)

0.7 μM

In vitro

Niclosamide (< 5 μM) dose dependently inhibits STAT3-mediated luciferase reporter activity with IC50 of 0.25 μM in HeLa cells. Niclosamide(< 2 μM) dose dependently inhibits the phosphorylation of STAT3 in Du145 cells. Niclosamide (1 μM) inhibits the EGF-induced nuclear translocation of STAT3 in Du145 cells. Niclosamide(< 2 μM) dose dependently inhibits the transcription of STAT3 downstream genes in Du145 cells. Niclosamide(< 10 μM) dose dependently induces G0/G1 arrest and apoptosis of Du145 cancer cells. ^[1] Niclosamide is able to inhibit SARS-CoV replication at a micromolar concentration in Vero E6 cells infected with SARS-CoV. ^[2] Niclosamide(< 7.5 μM) promotes Frizzled1 endocytosis, downregulates Dishevelled-2 protein, and inhibits Wnt3A-stimulated beta-catenin stabilization and LEF/TCF reporter activity in U2OS cells. ^[3] Niclosamide inhibits the TNF-induced NF-κB reporter activity in a dose- and time-dependent manner in U2OS cells. Niclosamide(125 nM) inhibits NF-κB activation induced by p65, IKKα, IKKβ, IKKγ, and TAK1 in U2OS cells. Niclosamide(< 500 nM) completely block the time- and dose-dependent TNFα-induced alteration of the NF-κB–DNA complex in HL-60 cells. Niclosamide(< 10 nM) inhibits constitutive NF-κB activation in U266 cells. Niclosamide inhibits TNF-induced degradation of IκBα and relocation of p65 in a dose- and time-dependent manner in HL-60, Molm13, or AML primary cells. Niclosamide(500 nM) decreases TNF-induced NF-κB–dependent gene products involved in cell survival in HL-60 cells. Niclosamide dose dependently inhibits the growth and induces robust apoptosis of AML cells associated with decreased Mcl-1 and XIAP levels and increased intracellular ROS levels. ^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
PC3	Mmf3RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MYCxNlAhcHK\|	NFPzZ41CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGDDN{Bk\WyuczDh[pRmeiBzMkCgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1yLkVOwG0>	MlrzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
A549	NV7zTllvSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		M4LERVEzOCCqcoO=	NX7mbIYxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEGyNEBpenNiYomgUXRVKGG\|c3H5MEBKSzVyPUCuOO69VQ>?	NW[4b\|BjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[2PFAyPTlpPkG2OlgxOTV7PD;hQi=>
U87MG	NWLOVYp5SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		M3PDRlEzOCCqcoO=	M3P5fGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iVUi3UWch[2WubIOgZYZ1\XJiMUKwJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MD60{txO	M{nKXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NkiwNVU6Lz5zNk[4NFE2QTxxYU6=
LoVo	NHvyS3JCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NXvMOFJpOTJyIHjydy=>	Mn\QRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCOb2\vJINmdGy\|IHHmeIVzKDF{MDDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRTBwN988US=>	M{PUUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NkiwNVU6Lz5zNk[4NFE2QTxxYU6=
MIAPaCa2	M1[3[WFvfGmycn;sbYZmemG2aY\lJIF{e2G7		MUSxNlAhcHK\|	MXrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2LQWDhR4Ez[2WubIOgZYZ1\XJiMUKwJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MT6x{txO	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjZ6MEG1PUc,OTZ4OECxOVk9N2F-
PC3	NF7ZSGNHfW6ldHnvckBie3OjeR?=		NYX5TmF3OSCqch?=	M1vVWWlvcGmkaYTpc44hd2ZibXn0c4Npd26mcnnhcEBu\W2kcnHu[UBxd3SnboTpZYwhcW5iaIXtZY4hWEN\|IHPlcIx{KGGodHXyJFEhcHJ?	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjZ6MEG1PUc,OTZ4OECxOVk9N2F-
HEK293	NHfLSoRHfW6ldHnvckBie3OjeR?=		MYqxJIhz	MlXETY5pcWKrdHnvckBw\iCvaYTvZ4hwdmS{aXHsJI1mdWK{YX7lJJBwfGWwdHnhcEBqdiCqdX3hckBJTUt{OUOgZ4VtdHNiYX\0[ZIhOSCqch?=	MkjtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
neural precursor	MWXGeY5kfGmxbjDhd5NigQ>?			NVXkSoI2UW6qaXLpeIlwdiCxZjDu[ZVzd3OyaHXy[UBxem:uaX\ldoF1cW:wIH;mJI1wfXOnIH7leZJidCCycnXjeZJ{d3JiY3XscJMh[nliTWTUJIF{e2G7	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzRzN{[zNUc,OTd2MUe2N\|E9N2F-
Vero E6	NYTOe2RZSW62aY\pdoFtKGG\|c3H5			NF\ydVNCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBUSVKVIHPvdo9v[X[rcoXzJIlvKF[ncn:gSVYh[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJidCC{ZYDsbYNifGmxbjDifUBGVEmVQTygSWM2ODxyLkJOwG0>	MlX0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4NkO1N\|koRjF5Nk[zOVM6RC:jPh?=
Vero E6	NXfPPHU5S3m2b4TvfIlkcXS7IHHzd4F6			M4rSNmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KF[ncn:gSVYh[2WubIOgZpkhVVSWIHHzd4F6NCCFQ{WwQVIzNjIQvF2=	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ4M{WzPUc,OTd4NkO1N\|k9N2F-
RAW264.7	MWDDfZRweHKxdHXjeIl3\SCjc4PhfS=>	NWXUSGJ1OTBidV2=		MWLDfZRweHKxdHXjeIl3\SCjY4Tpeol1gSCjZ3HpcpN1KGGwdHjyZZghfG:6aX6gcIV1cGGuIH\hZ5Rwei:ycn;0[YN1cX[nIHHueIlo\W5vaX7keYNm\CClZXzsJIRm[XSqIHnuJI1wfXOnIGLBW\|I3PC55IHPlcIx{KGG\|c3Xzd4VlKGG\|IHPlcIwhfmmjYnnsbZR6KGG2IEGwJJVOKGK7IF3UWEBz\WS3Y4Tpc44h[XO\|YYm=	NHT2Oog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUW0NFc3PCd-MUm1OFA4PjR:L3G+
CHO	NF\nVohEgXSxcILveIVkfGm4ZTDhd5NigQ>?			NUiwUmhrS3m2b4Dyc5Rm[3SrdnWgZYN1cX[rdImgZYdicW6\|dDDhcpRpemG6IH\1d4lwdiC2b4jpckBHWDV7LXnu[JVk\WRiY3XscEBl\WG2aDDpckBEUE9iY3XscJMh[XO\|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[nliTWTUJJJm\HWldHnvckBie3OjeR?=	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTV2MEe2OEc,OTl3NEC3OlQ9N2F-
CHO	M2XXd2Z2dmO2aX;uJIF{e2G7			MoHrTY5pcWKrdHnvckBw\iCEYXPpcIx2eyCjboTodoFkcXNiYX70bJJigCCycn;0[YN1cX[nIHHueIlo\W5iaHXweIFu\XJicILlMZBwemVidH:gdI9z\SClb372[ZJ{cW:wIHnuJGNOTzJvZYjwdoV{e2mwZzDDTG8h[2WubIO=	NWTEc\|JHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1OFA4PjRpPkG5OVQxPzZ2PD;hQi=>
Ava5	MUjBcpRqfmm{YXygZZN{[Xl?		M4\RVlMh\GG7cx?=	NGHYdXJCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJS1ZiZ3Xuc5R6eGViMXKgbY5n\WO2ZXSgbY4hSX[jNTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJJJmeGyrY3H0bY9vKGGodHXyJFMh\GG7czDifUBjdG:2IHj5ZpJq\Gm8YYTpc44h[W6jbInzbZMtKEWFNUC9NE4yPs7:TR?=	NXfIR\|dCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwOVk6QDNpPkKyNFU6QTh\|PD;hQi=>
Ava5	NXHDVY1jSW62aY\pdoFtKGG\|c3H5		NFrEUHE{KGSjeYO=	NIn3bWFCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJS1ZiZ3Xuc5R6eGViMXKgbY5n\WO2ZXSgbY4hSX[jNTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJJJmeGyrY3H0bY9vKGGodHXyJFMh\GG7czDifUBjdG:2IHj5ZpJq\Gm8YYTpc44h[W6jbInzbZMtKEWFOUC9NE44\|ryP	NELGclI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC1PVk5Oyd-MkKwOVk6QDN:L3G+
Ava5	NFfSSo9EgXSxdH;4bYNqfHliYYPzZZk>		M176[VMh\GG7cx?=	NVrybodmS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSX[jNTDj[YxteyCjZoTldkA{KGSjeYOgZpkhdmW3dILhcEBz\WRiZInlJIF{e2G7LDDDR\|UxRTFyzszN	NXfue5k1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwOVk6QDNpPkKyNFU6QTh\|PD;hQi=>
HFF	MYnBcpRq[XCrY3;tdIxmgGGwIHHzd4F6		Mn\aNlQhcHK\|	M3\I[2FvfGmjcHnjc41xdGW6YX6gZYN1cX[rdImgZYdicW6\|dDDUc5hweGyjc33hJIdwdmSraTDSTEB1[WOqeYrvbZRmeyCrbn\lZ5Rm\CCrbjDISmYh[2WubIOgZZN{\XO\|ZXSgZZMhYzOKXT31doFkcWxiaX7jc5Jxd3KjdHnvckBi\nSncjCyOEBpenNiYomgd4NqdnSrbHzheIlwdiCudX3pcoV{[2WwY3WgZ492dnSncjygUWlEQTB;MD6y{txO	MmjNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7N{C5N\|coRjJ{OUewPVM4RC:jPh?=
HFF	MUPBcpRq[XCrY3;tdIxmgGGwIHHzd4F6		NYn1eVAyOjRiaILz	NHHYbnRCdnSrYYDpZ49ueGyneHHuJIFkfGm4aYT5JIFo[Wmwc4SgWI95d3CuYYPtZUBod26maXmgVmghfGGlaIn6c4l1\XNiaX7m[YN1\WRiaX6gTGZHKGOnbHzzJIF{e2W\|c3XkJIF{KFt\|SG2teZJi[2muIHnuZ49zeG:{YYTpc44h[W[2ZYKgNlQhcHK\|IHL5JJNkcW62aXzsZZRqd25ibIXtbY5me2OnbnPlJINwfW62ZYKsJG1KSzVyPUCuNu69VQ>?	MnfhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7N{C5N\|coRjJ{OUewPVM4RC:jPh?=
MDA-MB-231	MoPRR5l1d3SxeHnjbZR6KGG\|c3H5			MljzR3l1d3SxeHnjbZR6KGGpYXnud5QhTVJvbnXnZZRqfmViaIXtZY4hVUSDLV3CMVI{OSClZXzsd{BjgSCPVGOgZZN{[XluIFnDOVA:OC55Od88US=>	M2LvS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEG2NVkyLz5{M{SxOlE6OTxxYU6=
MCF7	Mni1R5l1d3SxeHnjbZR6KGG\|c3H5			MkTYR3l1d3SxeHnjbZR6KGGpYXnud5QhTVJvcH;zbZRqfmViaIXtZY4hVUOINzDj[YxteyCkeTDNWHMh[XO\|YYmsJGlEPTB;MT6wOu69VQ>?	NYnBU4I3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NVYyQTFpPkKzOFE3OTlzPD;hQi=>
AsPC1	MUPDfZRwfG:6aXPpeJkh[XO\|YYm=			MnPFR3l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRZNRSzFiY3XscJMh[nliTWTTJIF{e2G7LDDJR\|UxRTFwNEhOwG0>	M{\FbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEG2NVkyLz5{M{SxOlE6OTxxYU6=
PANC1	MXPDfZRwfG:6aXPpeJkh[XO\|YYm=			Mm\uR3l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVGFPSzFiY3XscJMh[nliTWTTJIF{e2G7LDDJR\|UxRTFwN{ROwG0>	M1\WXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEG2NVkyLz5{M{SxOlE6OTxxYU6=
HEK293	Mkn5SpVv[3Srb36gZZN{[Xl?		NYjrd2pKQCCqcoO=	MXTJcohq[mm2aX;uJI9nKFewdEPBM4JmfGFvY3Hz[YlvKHOrZ37hcIlv\yCrbjDISWszQTNiY3XscJMh[W[2ZYKgPEBpenNiYomgWG9R\myjc3igdoVxd3K2ZYKgZZN{[XluIFnDOVA:OC52zszN	M4m1bFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEWzNFc{Lz5{M{S1N\|A4OzxxYU6=
U2OS	NEKwVpNHfW6ldHnvckBie3OjeR?=	NHXYOVMyOi53IIXN	MV62JIhzew>?	MWLJcoR2[3Srb36gc4YhcW62ZYLuZYxqgmG2aX;uJI9nKE[{aYr6cIVlOS2JRmCgLJVvc26xd36gc5Jq\2mwKTDlfJBz\XO\|ZXSgbY4hcHWvYX6gWVJQWyClZXzsd{BifCBzMj61JJVOKGGodHXyJFYhcHK\|IHL5JINwdm[xY3HsJI1q[3Kxc3PvdJk>	MkTTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NUOwO\|MoRjJ\|NEWzNFc{RC:jPh?=
MDA-MB-231	MoTzR5l1d3SxeHnjbZR6KGG\|c3H5		MWC3NkBpenN?	MY\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDFVkBv\WejdHn2[UBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHMh[XO\|YYmsJGlEPTB;MD63Pe69VQ>?	NUPaNVl6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVk3OTNpPkKzOFU6PjF\|PD;hQi=>
MCF7	MWPDfZRwfG:6aXPpeJkh[XO\|YYm=		MWW3NkBpenN?	MYTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDFVkBxd3OrdHn2[UBOS0Z5IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFNiYYPzZZktKEmFNUC9NU4xPs7:TR?=	NF3Od4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S1PVYyOyd-MkO0OVk3OTN:L3G+
AsPC1	NHrQfY9EgXSxdH;4bYNqfHliYYPzZZk>		NIPZcnQ4OiCqcoO=	NW\SfG5sS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSXOSQ{GgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WUzDhd5NigSxiSVO1NF0yNjR5zszN	NU\xWGI2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVk3OTNpPkKzOFU6PjF\|PD;hQi=>
PANC1	NILuOFhEgXSxdH;4bYNqfHliYYPzZZk>		M2DPTFczKGi{cx?=	M1nqcWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHBCVkNzIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFNiYYPzZZktKEmFNUC9NU44O87:TR?=	MorpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NUm2NVMoRjJ\|NEW5OlE{RC:jPh?=
MDA-MB-231	MX7BcpRqfHWvb4KgZZN{[Xl?	MnLaO\|UhdWdxa3e=	NV\VeItDPSCmYYnz	NFvvcYpCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDFVkBv\WejdHn2[UBOTEFvTVKtNlMyKGOnbHzzJJhmdm:pcnHmeIVlKGmwIH71[IUhdW:3c3WgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjD0eY1weiCpcn;3eIgh[XRiN{WgcYcwc2duIIDvJJFlKG[xcjC1JIRigXN?	NILs[JY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S1PVYyOyd-MkO0OVk3OTN:L3G+
MDA-MB-231	M1fUSGFvfGm2dX3vdkBie3OjeR?=	M{LFeFEzNjVibXevb4c>	MWG1JIRigXN?	NWnBd2hXSW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gSXIhdmWpYYTpeoUhVUSDLV3CMVI{OSClZXzsd{B5\W6xZ4Lh[pRm\CCrbjDueYRmKG2xdYPlJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[geJVud3JiZ4Lve5RpKGG2IEGyMlUhdWdxa3esJIlxKHGmIH\vdkA2KGSjeYO=	NEH3Rm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S1PVYyOyd-MkO0OVk3OTN:L3G+
MDA-MB-231	NX7BOmxlSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=	MVyxNEB1dyB{MDD1US=>	NHrGWZYzPCCqcoO=	NWLWfHV4SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDFVkBv\WejdHn2[UBOTEFvTVKtNlMyKGOnbHzzJIF1KDFyIITvJFIxKHWPIHHmeIVzKDJ2IHjyd{BjgSCPVGSgZZN{[Xl?	M3e3dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEW5OlE{Lz5{M{S1PVYyOzxxYU6=
MDA-MB-231	NGjCeGRHfW6ldHnvckBie3OjeR?=		NHfOclkzPCCqcoO=	NFTY[ZlKdmirYnn0bY9vKG:oIGPURXQ{KHC{b33veIVzKGGldHn2bZR6KGmwIHj1cYFvKEWUIH7l[4F1cX[nIF3ERU1OSi1{M{GgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KGS3YXygcJVkcW[ncnHz[UBz\XCxcoTldkBie3OjeR?=	M1nldVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEW5OlE{Lz5{M{S1PVYyOzxxYU6=
MDA-MB-231	NYnLWYxTTnWwY4Tpc44h[XO\|YYm=	MkH5NlAhfU1?	NGPrZ4wzPCCqcoO=	NYLRWYtqUW6qaXLpeIlwdiCxZjDTWGFVOyCycn;tc5RmeiCjY4Tpeol1gSCrbjDoeY1idiCHUjDu[YdifGm4ZTDNSGEuVUJvMkOxJINmdGy\|IHH0JFIxKHWPIHHmeIVzKDJ2IHjyd{BjgSCmdXHsJIx2[2moZYLhd4UhemWyb4L0[ZIh[XO\|YYm=	NWDqfmR6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVk3OTNpPkKzOFU6PjF\|PD;hQi=>
MDA-MB-231	NVXMcm06TnWwY4Tpc44h[XO\|YYm=	M4XQS\|ExKHWP	NFLwdlAzPCCqcoO=	M1e0NGlvcGmkaYTpc44hd2ZiU2TBWFMheHKxbX;0[ZIh[WO2aY\peJkhcW5iaIXtZY4hTVJibnXnZZRqfmViTVTBMW1DNTJ\|MTDj[YxteyCjdDCxNEB2VSCjZoTldkAzPCCqcoOgZpkh\HWjbDDseYNq\mW{YYPlJJJmeG:{dHXyJIF{e2G7	NEDzTGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S1PVYyOyd-MkO0OVk3OTN:L3G+
MDA-MB-231	NYnJb\|BXTnWwY4Tpc44h[XO\|YYm=	NXyzW29IOSC2bzCxNEB2VQ>?	MUKyOEBpenN?	M{nqNGlvcGmkaYTpc44hd2ZiU2TBWFMhcW5iaIXtZY4hTVJibnXnZZRqfmViTVTBMW1DNTJ\|MTDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gc4YhfG:2YXygV3RCXDNibHX2[Ywh[XRiMTD0c{AyOCC3TTDh[pRmeiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz	M{HUW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEW5OlE{Lz5{M{S1PVYyOzxxYU6=
MDA-MB-231	M3TjVmZ2dmO2aX;uJIF{e2G7	MXKxJJRwKDFyIIXN	NUiyXVNtPDhiaILz	M{DON2lv\HWldHnvckBw\iCvb4LwbI9td2erY3HsJINp[W6pZYOgbY4hcHWvYX6gSXIhdmWpYYTpeoUhVUSDLV3CMVI{OSClZXzsd{BifCBzIITvJFExKHWPIHHmeIVzKDR6IHjyd{BjgSCuaXfoeEBucWO{b4Pjc5Bq[yCjbnHsfZNqew>?	M17mNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEW5OlE{Lz5{M{S1PVYyOzxxYU6=
MDA-MB-231	M1;qR2Z2dmO2aX;uJIF{e2G7	NWrxfFBHOSC2bzCxNEB2VQ>?	MUW0PEBpenN?	NH70b\|FKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m\|IHnuJIh2dWGwIFXSJI5m\2G2aY\lJG1FSS2PQj2yN\|Eh[2WubIOgZZQhOSC2bzCxNEB2VSCjZoTldkA1QCCqcoOgZpkhdGmpaISgcYlkem:\|Y3;wbYMh[W6jbInzbZM>	NVrmXG04RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVk3OTNpPkKzOFU6PjF\|PD;hQi=>
MDA-MB-231	MULBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?	NFXkSmQyKHSxIEGwJJVO	MWK0PEBpenN?	MWfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEWUIH7l[4F1cX[nIF3ERU1OSi1{M{GgZ4VtdHNiYYSgNUB1dyBzMDD1UUBi\nSncjC0PEBpenNiYomgcIlocHRibXnjdo9{[2:yaXOgZY5idHm\|aYO=	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR3OU[xN{c,OjN2NUm2NVM9N2F-
MDA-MB-231	NUTNfnI5TnWwY4Tpc44h[XO\|YYm=	MnjONUB1dyBzMDD1US=>	MkPKNlQhcHK\|	MWLJcohq[mm2aX;uJI9nKFOWQWSzJIlvKGi3bXHuJGVTKG6nZ3H0bZZmKE2GQT3NRk0zOzFiY3XscJMh[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIH;mJJBpd3OyaH;yfYxifGWmIGPURXQ{KGG2IGT5dk04ODVibHX2[Ywh[XRiMTD0c{AyOCC3TTDh[pRmeiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz	NHnPTYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S1PVYyOyd-MkO0OVk3OTN:L3G+
HeLa	NF7lRZZHfW6ldHnvckBie3OjeR?=		MYWyOEBpenN?	NHLoOWtKdmirYnn0bY9vKG:oIGPURXQ{KGmwIHj1cYFvKEinTHGgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OD1yLkK1{txO	NV7ZdotrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFAzOzFpPkK0PVAxOjNzPD;hQi=>
DU145	M4jyUGFvfGmycn;sbYZmemG2aY\lJIF{e2G7		MXu3NkBpenN?	M{j5emFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iRGWxOFUh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1yLkhOwG0>	NETVUXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFI{OSd-MkS5NFAzOzF:L3G+
DU145	NXnJOWdJT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MUOxNUB1dyBzMjDkZZl{	MoHnS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gSHUyPDViY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBkd2yxbomg[o9zdWG2aX;uJIFnfGW{IEGxJJRwKDF{IHThfZMh[nliY4L5d5RidCC4aX;s[ZQhe3SjaX7pcoch[mG\|ZXSgcYlkem:\|Y3;wbYMh[W6jbInzbZMtKEmFNUC9NE44\|ryP	NYP5V4lqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFAzOzFpPkK0PVAxOjNzPD;hQi=>
HeLa	MV\BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NG\GR\|M4OiCqcoO=	NV3hVWhrSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MT60{txO	NV;B[XJ7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFAzOzFpPkK0PVAxOjNzPD;hQi=>
A549	NEL0VWRCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NWHqUop7PzJiaILz	NIPYO2VCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1\|zszN	NYL4fJd2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFAzOzFpPkK0PVAxOjNzPD;hQi=>
HT-29	NWDvd\|FMSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NV;WXIZUPzJiaILz	NH;FWXNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjUMVI6KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;Nz6y{txO	NUDUUVBuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFAzOzFpPkK0PVAxOjNzPD;hQi=>
A431	NHvFZ5pCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		M3vET\|czKGi{cx?=	M4D3W2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUSzNUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRThwON88US=>	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEKzNUc,OjR7MECyN\|E9N2F-
PC3	NIOwOJFCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		M3;0d\|czKGi{cx?=	M2LSUmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUFOzJINmdGy\|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OTFwN988US=>	Mo\rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MECyN\|EoRjJ2OUCwNlMyRC:jPh?=
HeLa	NFTVNIlHfW6ldHnvckBie3OjeR?=	NEjoOpQ2KHWP	M4jqZVI1KGi{cx?=	M4n5RWlvcGmkaYTpc44hd2ZiU2TBWFMhcW5iaIXtZY4hUGWOYTDj[YxteyCjdDC1JJVOKGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[Xl?	NYL5cGdTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFAzOzFpPkK0PVAxOjNzPD;hQi=>
DU145	MmD2SpVv[3Srb36gZZN{[Xl?	MXqxJJVO	M2T1[\|IhcHK\|	MkXGTY5pcWKrdHnvckBw\iCVVFHUN{BvfWOuZXHyJJRz[W6\|bH;jZZRqd25iaX6gSWdHKHO2aX31cIF1\WRiaIXtZY4hTFVzNEWgZ4VtdHNiYYSgNUB2VSCjZH3pcol{fGW{ZXSgZoVnd3KnIEGwNEBv\y:vbDDFS2Yhe3SrbYXsZZRqd25ibXXhd5Vz\WRiYX\0[ZIhOiCqcoOgZpkh[2:wZn;jZYwhdGG\|ZYKgcYlkem:\|Y3;wfS=>	MoLVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MECyN\|EoRjJ2OUCwNlMyRC:jPh?=
DU145	NI\zWmNHfW6ldHnvckBie3OjeR?=	M2XySFA2KHSxIEGwJJVO	MnjVNkBpenN?	NEPYPZpKdmirYnn0bY9vKG:oIGPURXQ{KGmwdHXyZYN1cW:wIIfpeIghTE6DIHLpcoRqdmdic3n0[UBqdiCqdX3hckBFXTF2NTDj[YxteyCjdDCwOUB1dyBzMDD1UUBi\nSncjCyJIhzeyCkeTDFUXNC	NVrm[4hMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFAzOzFpPkK0PVAxOjNzPD;hQi=>
human	M{PVU2Z2dmO2aX;uJIF{e2G7	MWWwMlEhfG9iMjD1US=>	MUSyJIhzew>?	M{W5R2Rm[3KnYYPlJIlvKGO7Y3zpckBFOSCycn;0[YlvKGyndnXsJIlvKGi3bXHuJGRWOTR3IHPlcIx{KGG2IECuNUB1dyB{IIXNJIFnfGW{IEKgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=>	Mn;KQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MECyN\|EoRjJ2OUCwNlMyRC:jPh?=
human	NFvyfVJHfW6ldHnvckBie3OjeR?=	MkjsNE4yKHSxIEKgeW0>	NUPMVVl[OiCqcoO=	Mo\pSIVkemWjc3WgbY4h[y2PeXOgdJJwfGWrbjDs[ZZmdCCrbjDoeY1idiCGVUG0OUBk\WyuczDheEAxNjFidH:gNkB2VSCjZoTldkAzKGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN?	Mke3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MECyN\|EoRjJ2OUCwNlMyRC:jPh?=
human	NH71c25HfW6ldHnvckBie3OjeR?=	MnHoNE4yKHSxIEKgeW0>	M3GzSlIhcHK\|	NHnTb4lF\WO{ZXHz[UBqdiCEY3ytXIwheHKxdHXpckBt\X[nbDDpckBpfW2jbjDEWVE1PSClZXzsd{BifCByLkGgeI8hOiC3TTDh[pRmeiB{IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm\|aYO=	NULDUGg{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFAzOzFpPkK0PVAxOjNzPD;hQi=>
DU145	NHfxZoZHfW6ldHnvckBie3OjeR?=	NIqzXnMxNjFidH:gNkB2VQ>?	Ml\YNkBpenN?	NITYVo1Fd3ewcnXneYxifGmxbjDv[kBO[2xvMTDwdo91\WmwIHnuJIh2dWGwIFTVNVQ2KGOnbHzzJIF1KDBwMTD0c{AzKHWPIHHmeIVzKDJiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?=	NYHmd5pwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFAzOzFpPkK0PVAxOjNzPD;hQi=>
human	M{O2UWZ2dmO2aX;uJIF{e2G7	MUmyJJVO	NEPmUZgzKGi{cx?=	NXrkT49HUW6qaXLpeIlwdiCxZjDTWGFVOyCyaH;zdIhwenmuYYTpc44h[XRiVInyO\|A2KGmwIHj1cYFvKESXMUS1JINmdGy\|IHH0JFIhfU1id3n0bIlvKDJiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?=	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEKzNUc,OjR7MECyN\|E9N2F-
breast cancer cells	NIrvb5ZEgXSxdH;4bYNqfHliYYPzZZk>		MUW3NkBpenN?	NUnmc4RCS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4h[nKnYYP0JINidmOncjDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB5MjDodpMh[nliQ3XscEBVcXSncjDHcI8hSXO\|YYmsJGlEPTB;MD6xN:69VQ>?	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ5MkCzNkc,OjZ{N{KwN\|I9N2F-
colon cancer cells	MorGR5l1d3SxeHnjbZR6KGG\|c3H5		NUO1cmJYPzJiaILz	NUXFV21bS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4h[2:ub36gZ4Fv[2W{IHPlcIx{KGG\|c3Xzd4VlKGG\|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDd{IHjyd{BjgSCFZXzsJHRqfGW{IFfsc{BCe3OjeTygTWM2OD1yLkGz{txO	NGDYcXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkK3NlA{Oid-Mk[yO\|IxOzJ:L3G+
lung cancer cells	MlrzR5l1d3SxeHnjbZR6KGG\|c3H5		MUO3NkBpenN?	NEC2Z45EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBtfW6pIHPhcoNmeiClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA4OiCqcoOgZpkhS2WubDDUbZRmeiCJbH:gRZN{[XluIFnDOVA:OC5zM988US=>	Ml\2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{N{KwN\|IoRjJ4MkeyNFMzRC:jPh?=
prostate cancer cells	MUXDfZRwfG:6aXPpeJkh[XO\|YYm=		MYS3NkBpenN?	NGTuUVVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBxem:\|dHH0[UBk[W6lZYKgZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiN{KgbJJ{KGK7IFPlcIwhXGm2ZYKgS4xwKEG\|c3H5MEBKSzVyPUCuNVPPxE1?	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ5MkCzNkc,OjZ{N{KwN\|I9N2F-
ovarian cancer cells	NFTxPIhEgXSxdH;4bYNqfHliYYPzZZk>		NEPPTmo4OiCqcoO=	MYPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDveoFzcWGwIHPhcoNmeiClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA4OiCqcoOgZpkhS2WubDDUbZRmeiCJbH:gRZN{[XluIFnDOVA:OC5zM988US=>	NIDRfGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkK3NlA{Oid-Mk[yO\|IxOzJ:L3G+
blood cancer cells	M1nyeWN6fG:2b4jpZ4l1gSCjc4PhfS=>		NHP3Rno4OiCqcoO=	NIXXO2dEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBjdG:xZDDjZY5k\XJiY3XscJMh[XO\|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPzJiaILzJIJ6KEOnbHygWIl1\XJiR3zvJGF{e2G7LDDJR\|UxRTBwMUROwG0>	MnvJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{N{KwN\|IoRjJ4MkeyNFMzRC:jPh?=
pancreatic cancer cells	M3qzXmN6fG:2b4jpZ4l1gSCjc4PhfS=>		MUO3NkBpenN?	NFTkT5pEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBx[W6lcnXheIlkKGOjbnPldkBk\WyuczDhd5Nme3OnZDDhd{Boem:5dHigbY5pcWKrdHnvckBi\nSncjC3NkBpenNiYomgR4VtdCCWaYTldkBIdG9iQYPzZZktKEmFNUC9NE4yO87:TR?=	MoX4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{N{KwN\|IoRjJ4MkeyNFMzRC:jPh?=
HEK293	NXTRb49RTnWwY4Tpc44h[XO\|YYm=		M3rtT\|ghcHK\|	MkT0TY5pcWKrdHnvckBw\iCZboSvZoV1[S2lYYTlcolvKGmwIFjFT\|I6OyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJHdvfDODLYP0bY12dGG2ZXSgWG9RTmyjc3igZYN1cX[rdImgZYZ1\XJiODDodpMtKEmFNUC9NE4{PM7:TR?=	MlTrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{N{KwN\|IoRjJ4MkeyNFMzRC:jPh?=
HCT116	M2TMV2N6fG:2b4jpZ4l1gSCjc4PhfS=>		MmjUO\|IhcHK\|	NGXEWXZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiN{KgbJJ{KGK7IF3UV{Bie3OjeTygTWM2OD1yLkS1{txO	NIPUOpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkK3NlA{Oid-Mk[yO\|IxOzJ:L3G+
U2OS	MlfCSpVv[3Srb36gZZN{[Xl?	M3nK[FEzNjVidV2=	M2fTV\|YhcHK\|	MVnJcohq[mm2aX;uJI9nKFewdD;i[ZRiNWOjdHXubY4hcW5iaIXtZY4hXTKRUzDj[YxteyCjc4Pld5Nm\CCjczDpcpRmem6jbHn6ZZRqd25ib3[g[pJqgnquZXStS2ZRKGG2IEGyMlUhfU1iYX\0[ZIhPiCqcoOgZpkh[2:wZn;jZYwhdWmlcn;zZ49xcWNiYX7hcJl{cXN?	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ5MkCzNkc,OjZ{N{KwN\|I9N2F-
HCT116	MW\GeY5kfGmxbjDhd5NigQ>?		NYToNZJuOyCqcoO=	MmrNSYZn\WO2IH;uJGFVWCCqb33lc5N1[XOrczDpckBpfW2jbjDIR3QyOTZiY3XscJMh[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJGFVWCCuZY\lcEBi\nSncjCzJIhzeyCrbjDhZpNmdmOnIH;mJIdtfWOxc3WgZpkhSVSSIHLpc4x2dWmwZYPj[Y51KGG\|c3H5MEBGSzVyPUCuNFU3\|ryP	MmWwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{M{O2PFAoRjJ6MkOzOlgxRC:jPh?=
HEK293	MXvGeY5kfGmxbjDhd5NigQ>?		MlLLOkBpenN?	NHT0[ZNKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5ic3nncoFtcW6pIDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKFewdEPBMZN1cW23bHH0[YQh[mW2YT3jZZRmdmmwIILld5BwdnOnIITyZY5{[3KrcITpc44h[W[2ZYKgOkBpenNiYomgWG9R\myjc3ig[JVidCCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGlEPTB;MD6xNu69VQ>?	MmPwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{M{O2PFAoRjJ6MkOzOlgxRC:jPh?=
HCT116	MUjGeY5kfGmxbjDhd5NigQ>?		NWnmd5U2OyCqcoO=	M1vLTmVn\mWldDDvckBCXFBiaH;t[Y9{fGG\|aYOgbY4hcHWvYX6gTGNVOTF4IHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBCXFBibHX2[Ywh[W[2ZYKgN{BpenNiaX6gZYJ{\W6lZTDv[kBodHWlb4PlJIJ6KEGWUDDibY9tfW2rbnXzZ4VvfCCjc4PhfUwhUUN3ME2wMlI2\|ryP	M1j5TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkOzOlgxLz5{OEKzN\|Y5ODxxYU6=
HCT116	MVXGeY5kfGmxbjDhd5NigQ>?	NGjRdnQ2KHWP	MWSxPEBpenN?	NFrXSZpKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5ic3nncoFtcW6pIHnuJIh2dWGwIFjDWFEyPiClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iCleYTvd49tcWNiYnX0ZU1k[XSnbnnuJJBzd3SnaX6g[ZhxemW\|c3nvckBifCB3IIXNJIFnfGW{IEG4JIhzeyCkeTDX[ZN1\XKwIHLsc5QhdWW2aH;k	NEjxXW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEKzN\|Y5OCd-MkiyN\|M3QDB:L3G+
HCT116	NYTYdmdQTnWwY4Tpc44h[XO\|YYm=	NH71T5E2KHWP	NHnNW5kyQCCqcoO=	NHXvOZFKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5ic3nncoFtcW6pIHnuJIh2dWGwIFjDWFEyPiClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iCleYTvd49tcWNiQYjpclIheHKxdHXpckBmgHC{ZYPzbY9vKGG2IEWgeW0h[W[2ZYKgNVghcHK\|IHL5JHdme3Sncn6gZoxwfCCvZYToc4Q>	NULaVVhrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiyN\|M3QDBpPkK4NlM{PjhyPD;hQi=>
HCT116	M3rJdGZ2dmO2aX;uJIF{e2G7	NHjzVo02KHWP	NVvqbYxzOThiaILz	NGfsRlRKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5ic3nncoFtcW6pIHnuJIh2dWGwIFjDWFEyPiClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iClLV35Z{Bxem:2ZXnuJIV5eHKnc4Ppc44h[XRiNTD1UUBi\nSncjCxPEBpenNiYomgW4V{fGW{bjDicI91KG2ndHjv[C=>	Mly1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{M{O2PFAoRjJ6MkOzOlgxRC:jPh?=
HCT116	NYmzNY9mTnWwY4Tpc44h[XO\|YYm=	NGTTcGI2KHWP	M4C2T\|E5KGi{cx?=	NGjWOG1KdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5ic3nncoFtcW6pIHnuJIh2dWGwIFjDWFEyPiClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iCFeXPsbY4hTDFicILveIVqdiCneIDy[ZN{cW:wIHH0JFUhfU1iYX\0[ZIhOThiaILzJIJ6KFenc4Tldo4h[myxdDDt[ZRpd2R?	NYHnW\|VGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiyN\|M3QDBpPkK4NlM{PjhyPD;hQi=>
HCT116	MnHBSpVv[3Srb36gZZN{[Xl?	M335SlUhfU1?	NUTGZWF1OThiaILz	MU\Jcohq[mm2aX;uJI9nKFewdD;i[ZRiNWOjdHXubY4he2mpbnHsbY5oKGmwIHj1cYFvKEiFVEGxOkBk\WyuczDhd5Nme3OnZDDhd{Bld3ewcnXneYxifGmxbjDv[kB{fXK4aY\pckBxem:2ZXnuJIV5eHKnc4Ppc44h[XRiNTD1UUBi\nSncjCxPEBpenNiYomgW4V{fGW{bjDicI91KG2ndHjv[C=>	NIOwPGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEKzN\|Y5OCd-MkiyN\|M3QDB:L3G+
SW480	NYLT[m9LTnWwY4Tpc44h[XO\|YYm=	MY[1JJVO	M37xVFE5KGi{cx?=	MoewTY5pcWKrdHnvckBw\iCZboSvZoV1[S2lYYTlcolvKHOrZ37hcIlv\yCrbjDoeY1idiCVV{S4NEBk\WyuczDhd5Nme3OnZDDhd{Bld3ewcnXneYxifGmxbjDv[kBkgXSxc3;sbYMh[mW2YT3jZZRmdmmwIIDyc5RmcW5iZYjwdoV{e2mxbjDheEA2KHWPIHHmeIVzKDF6IHjyd{BjgSCZZYP0[ZJvKGKub4SgcYV1cG:m	NHrac209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEKzN\|Y5OCd-MkiyN\|M3QDB:L3G+
SW480	Mn3OSpVv[3Srb36gZZN{[Xl?	MlrtOUB2VQ>?	M3rKRlE5KGi{cx?=	M3jMZmlvcGmkaYTpc44hd2ZiV370M4JmfGFvY3H0[Y5qdiC\|aXfuZYxqdmdiaX6gbJVu[W5iU2e0PFAh[2WubIOgZZN{\XO\|ZXSgZZMh\G:5boLl[5Vt[XSrb36gc4Yh[3m2b4PvcIlkKEG6aX6yJJBzd3SnaX6g[ZhxemW\|c3nvckBifCB3IIXNJIFnfGW{IEG4JIhzeyCkeTDX[ZN1\XKwIHLsc5QhdWW2aH;k	NX\VdIx{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiyN\|M3QDBpPkK4NlM{PjhyPD;hQi=>
SW480	MYHGeY5kfGmxbjDhd5NigQ>?	NGjIW2M2KHWP	M{PqTVE5KGi{cx?=	MVPJcohq[mm2aX;uJI9nKFewdD;i[ZRiNWOjdHXubY4he2mpbnHsbY5oKGmwIHj1cYFvKFOZNEiwJINmdGy\|IHHzd4V{e2WmIHHzJIRwf26{ZXf1cIF1cW:wIH;mJIMuVXmlIIDyc5RmcW5iZYjwdoV{e2mxbjDheEA2KHWPIHHmeIVzKDF6IHjyd{BjgSCZZYP0[ZJvKGKub4SgcYV1cG:m	Ml7IQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{M{O2PFAoRjJ6MkOzOlgxRC:jPh?=
SW480	MnPySpVv[3Srb36gZZN{[Xl?	MXW1JJVO	NFO2UWoyQCCqcoO=	M1nNR2lvcGmkaYTpc44hd2ZiV370M4JmfGFvY3H0[Y5qdiC\|aXfuZYxqdmdiaX6gbJVu[W5iU2e0PFAh[2WubIOgZZN{\XO\|ZXSgZZMh\G:5boLl[5Vt[XSrb36gc4YhS3mlbHnuJGQyKHC{b4TlbY4h\XiycnXzd4lwdiCjdDC1JJVOKGGodHXyJFE5KGi{czDifUBY\XO2ZYLuJIJtd3RibXX0bI9l	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDJ\|M{[4NEc,Ojh{M{O2PFA9N2F-
SW480	NEjNPGdHfW6ldHnvckBie3OjeR?=	NVnEZpFEPSC3TR?=	NUjpc2I4OThiaILz	MVLJcohq[mm2aX;uJI9nKFewdD;i[ZRiNWOjdHXubY4he2mpbnHsbY5oKGmwIHj1cYFvKFOZNEiwJINmdGy\|IHHzd4V{e2WmIHHzJIRwf26{ZXf1cIF1cW:wIH;mJJN2en[rdnnuJJBzd3SnaX6g[ZhxemW\|c3nvckBifCB3IIXNJIFnfGW{IEG4JIhzeyCkeTDX[ZN1\XKwIHLsc5QhdWW2aH;k	M2XGU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkOzOlgxLz5{OEKzN\|Y5ODxxYU6=
HCT116	NF6zNJlHfW6ldHnvckBie3OjeR?=	NFnTSoUzKHWP	M2jFZlMxKG2rboO=	NFLnN2RKdmS3Y4Tpc44hd2ZiQV3QT{BxcG:\|cHjvdplt[XSrb36gZZQhXGi{LUG3NkBz\XOrZIXlJIlvKGi3bXHuJGhEXDFzNjDj[YxteyCjdDCyJJVOKGGodHXyJFMxKG2rboOgbY4h\2y3Y3;z[UB{fXCybHXt[Y51\WRibXXkbYEh[nliaX3teY5w[myxdDDt[ZRpd2R?	NVzMb4V4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiyN\|M3QDBpPkK4NlM{PjhyPD;hQi=>
HCT116	NXrp[ZRjTnWwY4Tpc44h[XO\|YYm=	NIX4R\|QzKHWP	M3fTcVMxKG2rboO=	NHjCVnpKdmS3Y4Tpc44hd2ZiQV3QT{BxcG:\|cHjvdplt[XSrb36gZZQhXGi{LUG3NkBz\XOrZIXlJIlvKGi3bXHuJGhEXDFzNjDj[YxteyCjdDCyJJVOKGGodHXyJFMxKG2rboOgbY4h[WK\|ZX7j[UBw\iCpbIXjc5NmKGK7IHntcZVvd2Kub4SgcYV1cG:m	NVLRZpRzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiyN\|M3QDBpPkK4NlM{PjhyPD;hQi=>
U2OS	M2fsT2FvfGm4aYLhcEBie3OjeR?=			M{TnWWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGNpcWu3bnf1cpliKH[rcoXzJIlv\mWldHXkJIlvKGi3bXHuJHUzV1NiY3XscJMh[nliUmStdXBEWiCjbnHsfZNqeyxiRVO1NF0xNjN4zszN	M1XoVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6Nki5PVc2Lz5{OE[4PVk4PTxxYU6=
BHK-21	MX;BcpRqfmm{YXygZZN{[Xl?			NFvLSFFCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBEcGmtdX7neY56[SC4aYL1d{AxPjFzYWT3JIlv\mWldHXkJIlvKEKKSz2yNUBk\WyuczDifUBTXC2zUFPSJIFv[Wy7c3nzMEBGSzVyPUCuPFXPxE1?	NIS0So89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[4PVk4PSd-Mki2PFk6PzV:L3G+
BHK-21	M4\oPGFvfGm4aYLhcEBie3OjeR?=			M2HGdWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGNpcWu3bnf1cpliKH[rcoXzJFA5OTCkVIegbY5n\WO2ZXSgbY4hSkiNLUKxJINmdGy\|IHL5JHJVNXGSQ2KgZY5idHm\|aYOsJGVEPTB;MD65{txO	NYD5b41jRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2PFk6PzVpPkK4Olg6QTd3PD;hQi=>
BHK-21	NYrXbG44SW62aY\pdoFtKGG\|c3H5			MXvBcpRqfmm{YXygZYN1cX[rdImgZYdicW6\|dDDDbIlsfW6pdX75ZUB3cXK3czDpcoZm[3SnZDDpckBDUEtvMkGgZ4VtdHNiYomgVnQueVCFUjDhcoFtgXOrczygSWM2OD1yLkm1{txO	NGrJc2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[4PVk4PSd-Mki2PFk6PzV:L3G+
DAOY	MWfxTHRUKGG\|c3H5			NYLQ[llMeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IFTBU3kh[2WubIO=	Mn\VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
SJ-GBM2	NVW2eINIeUiWUzDhd5NigQ>?			NIn4dpByUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1qtS2JOOiClZXzsdy=>	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
A673	NEXqbFByUFSVIHHzd4F6			M2HZOpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCDNkezJINmdGy\|	MmexQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC	M1i3R5FJXFNiYYPzZZk>			MXfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?=	M3riXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
BT-37	MXPxTHRUKGG\|c3H5			NH\FcnFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQmStN\|ch[2WubIO=	NFu2NZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
NB-EBc1	Ml\2dWhVWyCjc4PhfS=>			NV3TUZFweUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IF7CMWVD[zFiY3XscJM>	NX3YVoFZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
Saos-2	NHvUdIFyUFSVIHHzd4F6			NHPsV4NyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU3Hvd{0zKGOnbHzz	NEPhbZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
SK-N-SH	M3nrUJFJXFNiYYPzZZk>			NH30[\|ZyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1UUCClZXzsdy=>	NFfQR4w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
NB1643	MXLxTHRUKGG\|c3H5			M4rHNpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCQQkG2OFMh[2WubIO=	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
LAN-5	MlOzdWhVWyCjc4PhfS=>			MXPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVEGQLUWgZ4VtdHN?	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
BT-12	MnyxdWhVWyCjc4PhfS=>			NHnyZZVyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQmStNVIh[2WubIO=	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
Rh18	MU\xTHRUKGG\|c3H5			MXfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmhzODDj[Yxtew>?	MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
OHS-50	MV3xTHRUKGG\|c3H5			MVnxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy\|	MlzSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
RD	MWrxTHRUKGG\|c3H5			NUe3c2F6eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IGLEJINmdGy\|	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
MG 63 (6-TG R)	NF7jS4lyUFSVIHHzd4F6			NHPQ[GJyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTVegOlMhMDZvVFegVkkh[2WubIO=	M17vU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh41	MWTxTHRUKGG\|c3H5			MV7xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmh2MTDj[Yxtew>?	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
NB1643	M1\sbJFJXFNiYYPzZZk>			NUHqN2RmeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhVkJzNkSzJINmdGy\|	M4\FTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
A673	M1rUSpFJXFNiYYPzZZk>			NUDIS3A3eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhSTZ5MzDj[Yxteyl?	MojhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC	NW[4NY5xeUiWUzDhd5NigQ>?			MXHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTT{1PNU2FIHPlcIx{	MoPmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
BT-12	M1;iUZFJXFNiYYPzZZk>			NFTadGdyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBDXC1zMjDj[Yxtew>?	MmGyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
LAN-5	NXLhSGtIeUiWUzDhd5NigQ>?			Mk\2dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUGFPNTViY3XscJM>	MmXaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
DAOY	M3Hpe5FJXFNiYYPzZZk>			M4LRbpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJGRCV1liY3XscJM>	NIfaWmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
NB-EBc1	MnGwdWhVWyCjc4PhfS=>			NWH5dnFIeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhVkJvRVLjNUBk\Wyucx?=	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
SJ-GBM2	NHywT3ZyUFSVIHHzd4F6			NEHuWW1yUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBUUi2JQl2yJINmdGy\|	M1nxTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
BT-37	Mm\PdWhVWyCjc4PhfS=>			MmnmdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgRnQuOzdiY3XscJM>	NFrZU2E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
TC32	MXrxTHRUKGG\|c3H5			NWPVUIRHeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhXEN\|MjDj[Yxtew>?	MkPwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
MG 63 (6-TG R)	MmPGdWhVWyCjc4PhfS=>			M{jCV5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJG1IKDZ\|IDi2MXRIKFJrIHPlcIx{	NWPURoRbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
fibroblast cells	M3u3U5FJXFNiYYPzZZk>			NWH0WXVKeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5Ih[2:wdILvcEBJcCC5aXzkJJR6eGViZnnido9jdGG\|dDDj[Yxtew>?	MkPzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
U-2 OS	NYfrem4xeUiWUzDhd5NigQ>?			M1nlSpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHUuOiCRUzDj[Yxtew>?	MortQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
Rh41	NHXiTnZyUFSVIHHzd4F6			MmHhdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgVog1OSClZXzsdy=>	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
RD	M2rmTJFJXFNiYYPzZZk>			NFzuSZNyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBTTCClZXzsdy=>	NFPMS4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
Rh18	NXfmTJNDeUiWUzDhd5NigQ>?			MlXVdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgVogyQCClZXzsdy=>	NU\W[oNkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
Rh30	M3PSZ5FJXFNiYYPzZZk>			MnHvdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgVog{OCClZXzsdy=>	NV7aPIdURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
Saos-2	MVjxTHRUKGG\|c3H5			MUHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTZY9{NTJiY3XscJM>	NFm0dW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
OHS-50	MUjxTHRUKGG\|c3H5			MWHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDPTHMuPTBiY3XscJM>	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
SK-N-SH	MoSxdWhVWyCjc4PhfS=>			NF3YeXFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBUUy2QLWPIJINmdGy\|	MoLkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
SK-N-SH	NYTneXBieUiWUzDhd5NigQ>?			M2TEXpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KE:{dHjv[49v[WxiM1Sgeoli[mmuaYT5JJNkemWnbjDmc5IhW0tvTj3TTEBk\Wyucx?=	NH;VSGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
SJ-GBM2	NFHxe2pyUFSVIHHzd4F6			MXLxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBQenSqb3fvcoFtKDOGII\pZYJqdGm2eTDzZ5Jm\W5iZn;yJHNLNUeETUKgZ4VtdHN?	NHTGZmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
Daoy	NX70[VQ1eUiWUzDhd5NigQ>?			NIj5VIFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCRcoToc4dwdmGuIEPEJJZq[WKrbHn0fUB{[3KnZX6g[o9zKESjb4mgZ4VtdHN?	NYjlfINVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
TC32	NUj2d4FkeUiWUzDhd5NigQ>?			NEnYXlJyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCRcoToc4dwdmGuIEPEJJZq[WKrbHn0fUB{[3KnZX6g[o9zKFSFM{KgZ4VtdHN?	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
MG 63 (6-TG R)	M4PZN5FJXFNiYYPzZZk>			NXHWfG9zeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiT4L0bI9od26jbDCzSEB3cWGkaXzpeJkhe2O{ZXXuJIZweiCPRzC2N{ApPi2WRzDSLUBk\Wyucx?=	NHL5S4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
RD	NEDCTVFyUFSVIHHzd4F6			NXe0SnhXeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiT4L0bI9od26jbDCzSEB3cWGkaXzpeJkhe2O{ZXXuJIZweiCURDDj[Yxtew>?	M{G4cFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SW948	MYnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NH3RS4Q4OiCqcoO=	MY\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFOZOUS4JINmdGy\|IHHmeIVzKDd{IHjyd{BjgSClb3zvdolu\XS{aXOgUXRUKGG\|c3H5MEBKSzVyPUCuNVHPxE1?	NU\ob401RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyO\|Q6OzlpPkOwNlc1QTN7PD;hQi=>
HT-29	Mn7XRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NHrRXoo4OiCqcoO=	M1PYSGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSGStNlkh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IHPvcI9zcW2ndILpZ{BOXFNiYYPzZZktKEmFNUC9NE4yO87:TR?=	NH;PWow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEK3OFk{QSd-M{CyO\|Q6Ozl:L3G+
HCT116	MlvaRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MV63NkBpenN?	NFvXdXlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjDWFEyPiClZXzsd{Bi\nSncjC3NkBpenNiYomgZ49td3KrbXX0dolkKE2WUzDhd5NigSxiSVO1NF0xNjRzzszN	NX7kW\|J{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyO\|Q6OzlpPkOwNlc1QTN7PD;hQi=>
HEK293	MUjGeY5kfGmxbjDhd5NigQ>?		NWPhNJpNPiCqcoO=	MlT6TY5pcWKrdHnvckBw\iCZboSzRU1{fGmvdXzheIVlKFewdD;i[ZRiNWOjdHXubY4heGG2aIfhfUBqdiCKRVuyPVMh[2WubIOgeJJidnOoZXP0[YQhf2m2aDDwPJhVV1CIbHHzbEwhWmWwaXzsZUBtfWOrZnXyZZNmKHCuYYPtbYQheFKOLWTLJIFv\CCyTFvPMlEh[W[2ZYKgOkBpenNiYomgSJVidCC2b4DmcIF{cCCudXPp[oVz[XOnIILldI9zfGW{IHHzd4F6NCCLQ{WwQVAvPDYQvF2=	NGnifHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEK3OFk{QSd-M{CyO\|Q6Ozl:L3G+
CRC240	MXjBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		M2XYclczKGi{cx?=	Mn:zRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCFUlOyOFAh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IHPvcI9zcW2ndILpZ{BOXFNiYYPzZZktKEmFNUC9NE45Qc7:TR?=	M3TpcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMke0PVM6Lz5\|MEK3OFk{QTxxYU6=
SW480	NXLUcYtISW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		MmCzO\|IhcHK\|	MmPpRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCVV{S4NEBk\WyuczDh[pRmeiB5MjDodpMh[nliY3;sc5JqdWW2cnnjJG1VWyCjc4PhfUwhUUN3ME2wMlk5\|ryP	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJ5NEmzPUc,OzB{N{S5N\|k9N2F-
DLD1	MnfQRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NGHsXY44OiCqcoO=	MWHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKESOREGgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGOxbH;ybY1mfHKrYzDNWHMh[XO\|YYmsJGlEPTB;Mj6zPe69VQ>?	M2HYc\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMke0PVM6Lz5\|MEK3OFk{QTxxYU6=
HCT116	MVLGeY5kfGmxbjDhd5NigQ>?	NFTtUVE2KHWP	NIn3N44yQCCqcoO=	NHXUXYlKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5ic3nncoFtcW6pIIDheIh4[XliaX6gbJVu[W5iSFPUNVE3KGOnbHzzJIN6fG:\|b3ygcJl{[XSnIHjhdoJwemmwZzDi[ZRiNWOjdHXubY4hdXW2YYTpc44h[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJGF5cW5{IIDyc5RmcW5iZYjwdoV{e2mxbjDheEA2KHWPIHHmeIVzKDF6IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm\|aYO=	NXTrUlBWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyO\|Q6OzlpPkOwNlc1QTN7PD;hQi=>
HCT116	NFTwSZhHfW6ldHnvckBie3OjeR?=	M37lelUhfU1?	MVyxPEBpenN?	NX;DOphLUW6qaXLpeIlwdiCxZjDXcpQw[mW2YT3jZZRmdmmwIIPp[45idGmwZzDwZZRpf2G7IHnuJIh2dWGwIFjDWFEyPiClZXzsd{BkgXSxc3;sJIx6e2G2ZTDoZZJjd3KrbnegZoV1[S2lYYTlcolvKG23dHH0bY9vKGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBj\XSjLXPheIVvcW5icILveIVqdiCneIDy[ZN{cW:wIHH0JFUhfU1iYX\0[ZIhOThiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?=	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJ5NEmzPUc,OzB{N{S5N\|k9N2F-
SW480	M3rqSmZ2dmO2aX;uJIF{e2G7	MVu1JJVO	MmfNNVghcHK\|	MVvJcohq[mm2aX;uJI9nKFewdD;i[ZRiNWOjdHXubY4he2mpbnHsbY5oKHCjdHj3ZZkhcW5iaIXtZY4hW1d2OECgZ4VtdHNiY4n0c5NwdCCueYPheIUhcGG{Yn;ybY5oKEGSQzDteZRifGmxbjDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iQYjpclIheHKxdHXpckBmgHC{ZYPzbY9vKGG2IEWgeW0h[W[2ZYKgNVghcHK\|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>?	Mn;HQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{N{S5N\|koRjNyMke0PVM6RC:jPh?=
SW480	NGLi[5pHfW6ldHnvckBie3OjeR?=	NVnWT5BCPSC3TR?=	NEXSd4cyQCCqcoO=	NXTBNZRtUW6qaXLpeIlwdiCxZjDXcpQw[mW2YT3jZZRmdmmwIIPp[45idGmwZzDwZZRpf2G7IHnuJIh2dWGwIGPXOFgxKGOnbHzzJIN6fG:\|b3ygcJl{[XSnIHjhdoJwemmwZzDBVGMhdXW2YYTpc44h[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJIJmfGFvY3H0[Y5qdiCycn;0[YlvKGW6cILld5Nqd25iYYSgOUB2VSCjZoTldkAyQCCqcoOgZpkhX2W\|dHXyckBjdG:2IHHuZYx6e2m\|	NGOwVWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEK3OFk{QSd-M{CyO\|Q6Ozl:L3G+
CRC240	MkXCSpVv[3Srb36gZZN{[Xl?	NEXiZpU2KHWP	M{C4RlE5KGi{cx?=	M1\Cc2lvcGmkaYTpc44hd2ZiV370M4JmfGFvY3H0[Y5qdiC\|aXfuZYxqdmdicHH0bJdigSCrbjDoeY1idiCFUlOyOFAh[2WubIOgZ5l1d3OxbDDsfZNifGViYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKEG6aX6yJJBzd3SnaX6g[ZhxemW\|c3nvckBifCB3IIXNJIFnfGW{IEG4JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM>	Mnf6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{N{S5N\|koRjNyMke0PVM6RC:jPh?=
CRC240	NXfBOYh5TnWwY4Tpc44h[XO\|YYm=	NGH4R\|c2KHWP	Mlz5NVghcHK\|	Mn21TY5pcWKrdHnvckBw\iCZboSvZoV1[S2lYYTlcolvKHOrZ37hcIlv\yCyYYToe4F6KGmwIHj1cYFvKEOUQ{K0NEBk\WyuczDjfZRwe2:uIHz5d4F1\SCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h[mW2YT3jZZRmdmmwIIDyc5RmcW5iZYjwdoV{e2mxbjDheEA2KHWPIHHmeIVzKDF6IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm\|aYO=	M4DwU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMke0PVM6Lz5\|MEK3OFk{QTxxYU6=
CCD-841-CoN	NIHrS4JHfW6ldHnvckBie3OjeR?=	M4jIUlEhfU1?		NXL3dWdLW3SrbYXsZZRqd25ib3[gcYl1d2Oqb37kdolidCC{ZYPwbZJifGmxbjDpckBpfW2jbjDDR2QuQDRzLVPvUkBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCxeInn[Y4h[2:wc4XtdJRqd25icnH0[UBifCBzIIXNJIlvKHC{ZYPlcoNmKG:oIF\DR3Ah[nlicH;sZZJw\3KjcHjpZ{BidmGueYPpdy=>	MkjBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{N{S5N\|koRjNyMke0PVM6RC:jPh?=
CCD-841-CoN	NVOxZ4JZTnWwY4Tpc44h[XO\|YYm=	NYDweVdYOC5zIITvJFExKHWP	NWPC[IZwOiCqcoO=	NXHZ[XJKUW6mdXP0bY9vKG:oIFHNVGsheGixc4Doc5J6dGG2aX;uJIlvKGi3bXHuJGNETC16NEGtR49PKGOnbHzzJIF1KDBwMTD0c{AyOCC3TTDh[pRmeiB{IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm\|aYO=	NVv0e21XRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyO\|Q6OzlpPkOwNlc1QTN7PD;hQi=>
CRC240	MnfNRY51cXS3bX;yJIF{e2G7	M{PQRVczKG2pL3vn		NFixNWZCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDDVmMzPDBiY3XscJMhgGWwb3fyZYZ1\WRiaX6gUm9FN1OFSVSgcY92e2ViYYSgO\|IhdWdxa3esJJBwKGGmbXnubZN1\XKnZDCxNUBl[Xm\|IHHu[EBu\WG\|dYLl[EBwdiCmYYmgOEwhQCCmdYLpcoch[2:vcH;1coQh\G:\|aX7nJIFv\CCmYYmgNVEheG:\|dDDsZZN1KGSxc3W=	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJ5NEmzPUc,OzB{N{S5N\|k9N2F-
U2OS	NGfQZphHfW6ldHnvckBie3OjeR?=		MX62JIhzew>?	MknGTY5lfWO2aX;uJI9nKEeIUD30ZYdo\WRiRorkNUApfW6tbn;3ckBwemmpaX6pJIlvfGW{bnHsbZpifGmxbjDlfJBz\XO\|ZXSgbY4hcHWvYX6gWVJQWyClZXzsd{BifCBzMj61JIFnfGW{IE[gbJJ{KGK7IHPvcoZw[2GuIH3pZ5Jwe2OxcHnjJI1mfGixZB?=	Mn\jQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{N{S5N\|koRjNyMke0PVM6RC:jPh?=
A549	NVfFe\|l4TnWwY4Tpc44h[XO\|YYm=		MXW2JIhzew>?	M1O0c2lv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iQUW0PUBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiClYYPwZZNmKDNxNzDhZ5Rqfmm2eTDh[pRmeiB4IHjyd{BjgSClYYPwZZNmKGeubzCzM\|ch[XO\|YYm=	M4LudlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyM{exNFY1Lz5\|MEO3NVA3PDxxYU6=
Caco2	NGf5dHRHfW6ldHnvckBie3OjeR?=		MX[2JIhzew>?	MUXJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKEOjY3:yJINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGOjc4Dhd4UhOy95IHHjeIl3cXS7IHHmeIVzKDZiaILzJIJ6KGOjc4Dhd4Uh\2yxIEOvO{Bie3OjeR?=	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODN5MUC2OEc,OzB\|N{GwOlQ9N2F-
AsPC1	M{j6dWZ2dmO2aX;uJIF{e2G7		MY[2JIhzew>?	NG\POmNKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m\|IHnuJIh2dWGwIFHzVGMyKGOnbHzzJIF{e2W\|c3XkJIF{KGmwY4LlZZNmKGmwIHPhd5Bie2ViMz:3JIFkfGm4aYT5JIFnfGW{IE[gbJJ{KGK7IHPhd5Bie2ViZ3zvJFMwPyCjc4PhfS=>	MoXlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB\|N{GwOlQoRjNyM{exNFY1RC:jPh?=
HEK293	MlrISpVv[3Srb36gZZN{[Xl?		M3jucVghcHK\|	NEnWOY5KdmirYnn0bY9vKG:oIGfueFNCNXO2aX31cIF1\WRiV370M4JmfGFvY3H0[Y5qdiCyYYToe4F6KGmwIFjFT\|I6OyClZXzsd{B1emGwc3\lZ5Rm\CC5aYToJJA5gFSRUF\sZZNpNCCUZX7pcIxiKGy3Y3nm[ZJie2VicHzhd41q\CCyUlytWGsh[W6mIIDMT28vOSCjZoTldkA5KGi{czDifUBFfWGuIITvdIZt[XOqIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3ME2wMlM1\|ryP	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODV3MUmwNUc,OzB3NUG5NFE9N2F-
LN229	MVTBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		M322dVUh\GG7cx?=	NGrP[oRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFzONlI6KGOnbHzzJIFnfGW{IEWg[IF6eyCkeTDBcIFu[XKEbIXlJIF{e2G7LDDJR\|UxRTBwM988US=>	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODV6M{K0PEc,OzB3OEOyOFg9N2F-
T98G	NU\p[Io6SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		Mn7vOUBl[Xm\|	NVf3e5hkSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDUPVhIKGOnbHzzJIFnfGW{IEWg[IF6eyCkeTDBcIFu[XKEbIXlJIF{e2G7LDDJR\|UxRTBwM988US=>	MlzJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB3OEOyOFgoRjNyNUizNlQ5RC:jPh?=
U87	MXHBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		M{XrcFUh\GG7cx?=	MVzBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFV6NzDj[YxteyCjZoTldkA2KGSjeYOgZpkhSWyjbXHyRox2\SCjc4PhfUwhUUN3ME2wMlPPxE1?	NHX4Xmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEW4N\|I1QCd-M{C1PFMzPDh:L3G+
U138MG	Mn;kRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MYO1JIRigXN?	Mo\WRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCXMUO4UWch[2WubIOgZYZ1\XJiNTDkZZl{KGK7IFHsZY1iekKudXWgZZN{[XluIFnDOVA:OC5\|zszN	NXq4cJQ3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C1PFMzPDhpPkOwOVg{OjR6PD;hQi=>
U373	NYPJR2UySW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		MUi1JIRigXN?	MXTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFV\|N{OgZ4VtdHNiYX\0[ZIhPSCmYYnzJIJ6KEGuYX3hdmJtfWViYYPzZZktKEmFNUC9NE4{\|ryP	MkLJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB3OEOyOFgoRjNyNUizNlQ5RC:jPh?=
HL60	MXXDfZRwfG:6aXPpeJkh[XO\|YYm=		NISzNos{KGSjeYO=	M3LzPGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNPjBiY3XscJMhcW6ldXLheIVlKG[xcjCzJIRigXNiYomgR4VtdFSrdHXyMWdtdyCudX3pcoV{[2WwdDDhd5NigSxiSVO1NF0xNjQQvF2=	NVXofpl6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{GyOVM2OjlpPkOxNlU{PTJ7PD;hQi=>
KG1	NFHHdGpEgXSxdH;4bYNqfHliYYPzZZk>		MmS5N{Bl[Xm\|	MXLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLS\|Eh[2WubIOgbY5kfWKjdHXkJIZweiB\|IHThfZMh[nliQ3XscHRqfGW{LVfsc{BtfW2rbnXzZ4VvfCCjc4PhfUwhUUN3ME2wMlM3\|ryP	MnPEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF{NUO1NlkoRjNzMkWzOVI6RC:jPh?=
Jurkat	M1jXcmN6fG:2b4jpZ4l1gSCjc4PhfS=>		MVuzJIRigXN?	Mm\QR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTpVzc2G2IHPlcIx{KGmwY4XiZZRm\CCob4KgN{Bl[Xm\|IHL5JGNmdGyWaYTldk1IdG9ibIXtbY5me2OnboSgZZN{[XluIFnDOVA:OC52zszN	NFzUc4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MUK1N\|UzQSd-M{GyOVM2Ojl:L3G+
NALM6	MUnDfZRwfG:6aXPpeJkh[XO\|YYm=		M3;uWlMh\GG7cx?=	NGDFXnpEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBPSUyPNjDj[YxteyCrbnP1ZoF1\WRiZn;yJFMh\GG7czDifUBE\WyuVHn0[ZIuT2yxIHz1cYlv\XOlZX70JIF{e2G7LDDJR\|UxRTBwNkNOwG0>	NXvwfm5bRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{GyOVM2OjlpPkOxNlU{PTJ7PD;hQi=>
HEK293	NXniWmdyTnWwY4Tpc44h[XO\|YYm=		NF6zOGg6OCCvaX7z	M1vQcmlvcGmkaYTpc44hd2ZiS1nYMXJNfWOQIH\1d4VlKEOEUDCoeY5sdm:5bjDvdolocW5rIHLpcoRqdmdidH:gT2lFNVKOdXPDJIZ2e2WmIFPSSWIhMHWwa37ve44hd3KrZ3nuLUB1emGwc3\lZ5Rm\CCrbjDoeY1idiCKRVuyPVMh[2WubIOgdJJmcW6ldXLheIVlKHerdHigZ49ueG:3bnSgN\|AhdWmwczDi[YZwemViYnXmc5JmKG[xcoPrc4xqdiCjZHTpeIlwdiCjbnSgcYVie3W{ZXSgZYZ1\XJiOUCgcYlveyCrbjDwdoV{\W6lZTDv[kBkd2WuZX70[ZJigixiSVO1NF0yNjV\|zszN	Mn72QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF{NUO1NlkoRjNzMkWzOVI6RC:jPh?=
293beta5	M1fF[GFvfGm4aYLhcEBie3OjeR?=		MmDnO{Bl[Xm\|	MonYRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTJVu[W5iYXTlco93cXK3czC1JIlv\mWldHXkJIlvKDJ7M3LleIE2KGOnbHzzJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[gdIxieXWnIH\vdo1ifGmxbjDpcoN2[mG2ZXSg[o9zKDdiZHH5d{BjgSCJRmCgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3ME2wMlbPxE1?	NHPRWYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MkC0OVI{QSd-M{KwOFUzOzl:L3G+
A549	M2XKe2FvfGm4aYLhcEBie3OjeR?=		MUW0PEBpenN?	NEP6SGhCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJfW2jbjDh[IVvd3[rcoXzJFUhcW6oZXP0[YQhcW5iaIXtZY4hSTV2OTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJIVvfHK7IHHmeIVzKDR6IHjyd{BjgSCJRmCgdoVxd3K2ZYKg[4Vv\SCkYYPl[EBie3OjeTygTWM2OD1zLkKy{txO	NVfRNmk1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{KwOFUzOzlpPkOyNFQ2OjN7PD;hQi=>
A549	M1LE[mN6fG:2b4jpZ4l1gSCjc4PhfS=>		MWG0PEBpenN?	NFjGZ5VEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliYXzhcYFzKGKudXWgZZN{[XluIFPDOVA:OjJwOd88US=>	NVTBdnpSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{KwOFUzOzlpPkOyNFQ2OjN7PD;hQi=>
Vero	NVjJenVPTnWwY4Tpc44h[XO\|YYm=			NGGwTmZX\XKxIHPlcIx{KH[rYXLpcIl1gSCzSGTTJIZweiCcaXvhJJZqenW\|IHnubIljcXSxcoO=	M3;CTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzN\|MkK5OVQ2Lz5\|M{KyPVU1PTxxYU6=
Vero	M37NRWFvfGm4aYLhcEBie3OjeR?=		MV:yOEBpeg>?	M1LXbGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JHNCWlNvQ3;WMVIhMH[rcnHsJJRqfGW{KTDt[YF{fXKnZDDifUBxdGGzdXWgZZN{[XliaX6gWoVzdyClZXzsd{BifCCPT1mgNE4xOTJ3IHHmeIVzKDJ2IHjyMEBKSzVyPUCuNljPxE1?	Mo[zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ1QC9pPlPoSW1DVDxxYU6=
skeletal myoblast cells	MY\DfZRwfG:6aXPpeJkh[XO\|YYm=		NUGyfYdmPzJiaB?=	MlS2SG5FUTpiQ4n0c5RwgGmlaYT5JIlvKF[rdILvMEA4OiCqb4XyMEBqdiC{YYSgd4tmdGW2YXygcZlw[myjc4SgZ4VtdHNuIFnDOVA:Oi5\|zszN	M3ewV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PDhxJ{7DbGVOSkx:L3G+
VERO-E6	NILZbW9HfW6ldHnvckBie3OjeR?=		MkT4OFghcHK\|	MXXE[ZRmem2rbnH0bY9vKG:oIFnDOVAhfmGudXXzJIZweiCrbnjpZol1cW:wIH;mJHNCWlNvQ3;WMVIhcW6mdXPl[EBkgXSxdH;4bYNqfHlib3[gWmVTVy2HNjDj[YxteyCjZoTldkA1QCCqb4Xyd{Ah\Xiyb4P1doUhfG9iMD6wNUBOV0liU1HSV{BEd1ZvMjD2bZJ2eyCkeTDobYdpKGOxboTlcpQhcW2jZ3nu[{whUUN3ME20MlHPxE1?	MoG5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ1QC9pPlPoSW1DVDxxYU6=

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	p-STAT3 / STAT3 / c-Myc / Survivin ; PubMed: 25970160 Prostate LNCaP, C4-2B or DU145 cells were treated with DMSO, 0.5 μM or 1 μM niclosamide overnight, cells were then stimulated with 10 ng/mL of IL6 for 30 minutes. Whole cell lysates were prepared and subjected to Western blot analysis using antibodies as indicated. p-BCR-ABL / BCR-ABL ; PubMed: 29358661 Cell Death Dis KBM5 cells harboring wild-type or T315I-BCR-ABL and K562 cell were exposed to different concentrations of niclosamide, and then analyzed by Western blotting analysis. p-STAT5 / STAT5 / p-AKT / AKT / p-ERK / ERK ; PubMed: 29358661 Cell Death Dis CML cells were treated with increasing concentrations of niclosamide for 48 h and subjected to Western blotting analysis. Actin served as a loading control for blots above, which were performed sequentially. β-catenin; PubMed: 27652012 Springerplus Niclosamide decreases intracellular β-catenin levels in A-498 and SW-839 cells. Cells were treated with niclosamide for 24 h prior to WB analysis.	25970160 29358661 27652012
Growth inhibition assay	Cell viability; PubMed: 28418862 Oncotarget Cell viability of colon cancer cells after treatment of niclosamide for a 48-h period. Cell viability was determined by trypan blue exclusion assay from triplicate experiments.	28418862

In vivo

Niclosamide(40 mg/kg/d, i.p.) inhibits growth of xenografted AML cells in nude mice bearing HL-60 xenograft tumors. ^[4]

Protocol

Kinase Assay: ^[1]	- Collapse Protein Kinase profiling assay: Assay for 22 different proteins kinases is carried out by ProQinase Gmbh. All of the protein kinases are expressed either in Sf9 insect cells or in E.coli as recombinant GST-fusion proteins or His-tagged proteins. Protein kinases are purified by affinity chromatography using either GSH-agarose or Ni_NTH-agarose. A radiometric protein kinase assay is used for measuring the kinase activity of the 22 protein kinases. Briefly, for each protein kinase, 50 μL reaction cocktail containing 60 mM HEPES-NaOH, 3 mM MgCl₂, 3 mM MnCl₂, 3 μM Na-orthovanadate, 1.2 mM DTT, 50 μg/mL PEG20000, 1 μM [γ-33P]-ATP, Niclosamide, adequate amount of enzyme and its substrate. The PKC-alpha assay additionally contain 1 mM Cacl₂, 4 mM EDTA, 5 μg/mL phosphatidylserine and 1 μg/mL 1, 2-Dioleyl-glycerol. The reaction cocktails are incubated at 37 °C for 60 minutes and stop with 50 μL 2% (v/v) H₃PO₄. Incorporation of 33Pi is determined with a microplate scintillation counter. The activities and the IC50 values are calculated using Quattro Workflow V2.28.
Cell Research: ^[1]	- Collapse Cell lines: Hela, A549, Du145, HT-29, A431, PC3 cells Concentrations: 16 μM Incubation Time: 72 hours Method: Cells are plated in 96-well culture plates with cell density of 3-4 × 10³ cells/well and treat with Niclosamide by adding 100 μL medium containing Niclosamide of various concentrations on the second day. After 72-hour's treatment, MTT is added to each well and incubated for additional 4-5 hours, and the absorbance is measured on a microplate reader at 570nm. Cell growth inhibition is evaluated as the ratio of the absorbance of the sample to that of the control. The results are representative of at least 3 independent experiments. (Only for Reference)
Animal Research: ^[4]	- Collapse Animal Models: nude mice bearing HL-60 xenograft tumors. Dosages: 40 mg/kg Administration: Intraperitoneal injection (Only for Reference)

References

[4] Jin Y, et al. Cancer Res, 2010, 70(6), 2516-2527.

- Collapse

Solubility (25°C)

In vitro	DMF	12 mg/mL warmed (36.68 mM)
	DMSO	Insoluble
	Water	Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Niclosamide (BAY2353) SDF

Molecular Weight	327.12
Formula	C₁₃H₈Cl₂N₂O₄
CAS No.	50-65-7
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	Niclocide, NSC 178296
Smiles	C1=CC(=C(C=C1[N+](=O)[O-])Cl)NC(=O)C2=C(C=CC(=C2)Cl)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO+ % + % Tween 80+ % ddH₂O

Calculate Reset

Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2021-09-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04436458	Not yet recruiting	Drug: Niclosamide Oral Tablet\|Drug: Placebo	COVID	First Wave Bio Inc.	June 2021	Phase 2
NCT04644705	Recruiting	Drug: Niclosamide\|Drug: Placebo	Healthy Volunteers	Charité Research Organisation GmbH\|Bayer	November 2 2020	Phase 1
NCT04592835	Not yet recruiting	Drug: DWRX2003	COVID-19 Patients	Daewoong Pharmaceutical Co. LTD.\|Novotech (Australia) Pty Limited	October 19 2020	Phase 1
NCT04524052	Not yet recruiting	Drug: DWRX2003\|Drug: Placebo	Healthy	Daewoong Pharmaceutical Co. LTD.	August 2020	Phase 1
NCT03521232	Recruiting	Drug: Niclosamide	Ulcerative Colitis\|Ulcerative Proctitis\|Ulcerative Proctosigmoiditis	First Wave Bio Inc.	May 15 2018	Phase 1\|Phase 2
NCT02687009	Terminated	Drug: Niclosamide	Colon Cancer	Michael Morse MD\|Duke University	November 7 2017	Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

STAT Signaling Pathway Map

Related STAT Products

Cerdulatinib (PRT062070) hydrochloride ^New

Cerdulatinib (PRT-062070, PRT2070) hydrochloride is an oral active, multi-targeted tyrosine kinase inhibitor with IC50 of 12 nM/6 nM/8 nM/0.5 nM and 32 nM for JAK1/JAK2/JAK3/TYK2 and Syk, respectively. Also inhibits 19 other tested kinases with IC50 less than 200 nM.
FLLL32 ^New

FLLL32 is a potent JAK2/STAT3 inhibitor with IC50 of <5 μM. FLLL32 inhibits the induction of STAT3 phosphorylation by IFNα and IL-6 in breast cancer cells.
S3I-201

S3I-201 (NSC 74859) shows potent inhibition of STAT3 DNA-binding activity with IC50 of 86 μM in cell-free assays, and low activity towards STAT1 and STAT5.

Features:A chemical probe inhibitor of Stat3 activity.
Fludarabine (NSC 118218)

Fludarabine (NSC 118218, FaraA, Fludarabinum) is a STAT1 activation inhibitor which causes a specific depletion of STAT1 protein (and mRNA) but not of other STATs. Also a DNA synthesis inhibitor in vascular smooth muscle cells. Fludarabine induces apoptosis.
Cryptotanshinone

Cryptotanshinone is a STAT3 inhibitor with IC50 of 4.6 μM in a cell-free assay, strongly inhibits phosphorylation of STAT3 Tyr705, with a small effect on STAT3 Ser727, but none against STAT1 nor STAT5. Cryptotanshinone induces ROS-dependent autophagy and mitochondria-mediated apoptosis.
Stattic

Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1. Stattic induces apoptosis.

Features:Stattic is the first non-peptide small molecule with inhibitory activity against STAT3 SH2 domain regardless of the STAT3 phosphorylation state in vitro.
Nifuroxazide

Nifuroxazide is a cell-permeable and orally available nitrofuran-based antidiarrheal agent that effectively suppresses the activation of cellular STAT1/3/5 transcription activity with IC50 of 3 μM against IL-6-induced STAT3 activation in U3A cells.
HO-3867

HO-3867, an analog of curcumin, is a selective STAT3 inhibitor that inhibits its phosphorylation, transcription, and DNA binding without affecting the expression of other active STATs. HO-3867 induces apoptosis.

Choose Your Country or Region

By Signaling Pathways

By product type

Niclosamide (BAY2353)