For research use only. Not for use in humans.

Catalog No.S3030 Synonyms: Niclocide

12 publications

Niclosamide Chemical Structure

Molecular Weight(MW): 327.12

Niclosamide can inhibit DNA replication and inhibit STAT3 with IC50 of 0.7 μM in a cell-free assay. Niclosamide selectively inhibited the phosphorylation of STAT3 and had no obvious inhibition against the activation of other homologues (e.g., STAT1 and STAT5).

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Selleck's Niclosamide has been cited by 12 publications

6 Customer Reviews

  • Effects of some confirmed hits on IRF7 transcription level in response to IFN-α2a treatment (1 h) in SH-SY5Y cells. Data represent mean expression fold±SEM relative to GAPDH, measured from three independent experiments, each in triplicates. *P<0.05, **P<0.01, and ***P<0.001 compared to IFN-α2a treated cells.

    Acta Pharm Sin B, 2018, 8(6):889-899. Niclosamide purchased from Selleck.

    (B) H&E images. (C–E) IHC for PCNA, pSTAT3, and pPI3K after the drug treatment. BKM120 treatment showed downregulation of the pPI3K expression. pSTAT3 expression, however, was not completely suppressed by STAT3 inhibitor treatment. Note that pSTAT3 and PCNA were heavily expressed in the same dysplastic area of serial sections (red circles, sections from the same liver tissue). (F) ISH for Appa showing downregulation by STAT3 inhibitors. Bars, 50 μm.

    Neoplasia, 2015, 17(7):586-97. Niclosamide purchased from Selleck.

  • LAMP1 IF analysis. (A) Representative confocal IF images (60×) of LAMP1 (green) in SK-GT-4 cells or (C) FLO-1 cells treated with vehicle or niclosamide (0.5 μM) for 48 hours. Arrows point to cells with peripheral lysosomes, while arrowheads indicate cells with perinuclear lysosomes. (B) Quantification of the percentage of SK-GT-4 cells or (D) FLO-1 cells with either peripheral or perinuclear lysosomes relative to their respective total cell number after treatment with vehicle or niclosamide.

    Neoplasia, 2018, 20(10):1008-1022. Niclosamide purchased from Selleck.

    Bacterial infection in IPEC-J2 cells. The invasion and attachment of EHECO157:H7 was increased in the IPEC-J2 cells in the presence of 1 μM niclosamide. Data are expressed as the mean ± SEM (n= 6). Differences between groups were determined by paired samples t-test. *P<0.05 compared with the control.

    Int Immunopharmacol, 2016, 36:199-204.. Niclosamide purchased from Selleck.

  • Inhibition of STAT3 phosphorylation by niclosamide in colon cancer cells HCT116 and SW620. Notes: (A) SW620 cells were treated with niclosamide (5 µM) for different lengths of time (0, 2, 4, 6, 8, and 12 h). Total protein was extracted, and the expression levels of P-STAT3, STAT3, and GAPDH proteins were detected by Western blot analysis. (B) HCT116 cells were treated with niclosamide (5 µM) for different lengths of time (0, 2, 4, 6, 8, and 12 h). Total protein was extracted, and the expression levels of P-STAT3, STAT3, and GAPDH proteins were detected by Western blot analysis. (C) SW620 cells were treated with niclosamide at different concentrations (0.5, 1, 2.5, 5, and 10 µM) or vehicle control (DMSO) for 12 h. (D) HCT116 cells were treated with niclosamide at different concentrations (0.5, 1, 2.5, 5, and 10 µM) or vehicle control (DMSO) for 12 h. Total protein was then extracted and detected by Western blot analysis. The data were obtained from 3 independent experiments. *P<0.05.

    Onco Targets Ther, 2017, 10:1767-1776. Niclosamide purchased from Selleck.

    STAT 3 inhibitor, niclosamide, had the antitumor activity for ovarian clear cell cancer cell lines, KK. (A) Niclosamide had antitumor effect for ovarian clear-cell carcinoma cell line, KK. (B) Western blot analysis revealed the downregulation of p-STAT3, and XIAP proteins and increased expression of cleaved-PARP by treatment with niclosamide in a dose-dependent manner. Cell viability was assessed using MTT assay after 24 h from the treatment with niclosamide. Equivalent amounts (10 µg) of protein were subjected to SDS-PAGE and blotted with anti-STAT3, anti-phospho-STAT3, anti-XIAP, anti-cleaved-PARP, and anti-β-actin antibodies.

    Oncol Lett, 2018, 15(4):5772-5780. Niclosamide purchased from Selleck.

Purity & Quality Control

Choose Selective STAT Inhibitors

Biological Activity

Description Niclosamide can inhibit DNA replication and inhibit STAT3 with IC50 of 0.7 μM in a cell-free assay. Niclosamide selectively inhibited the phosphorylation of STAT3 and had no obvious inhibition against the activation of other homologues (e.g., STAT1 and STAT5).
STAT3 [1]
(in Hela cells)
0.7 μM
In vitro

Niclosamide (< 5 μM) dose dependently inhibits STAT3-mediated luciferase reporter activity with IC50 of 0.25 μM in HeLa cells. Niclosamide(< 2 μM) dose dependently inhibits the phosphorylation of STAT3 in Du145 cells. Niclosamide (1 μM) inhibits the EGF-induced nuclear translocation of STAT3 in Du145 cells. Niclosamide(< 2 μM) dose dependently inhibits the transcription of STAT3 downstream genes in Du145 cells. Niclosamide(< 10 μM) dose dependently induces G0/G1 arrest and apoptosis of Du145 cancer cells. [1] Niclosamide is able to inhibit SARS-CoV replication at a micromolar concentration in Vero E6 cells infected with SARS-CoV. [2] Niclosamide(< 7.5 μM) promotes Frizzled1 endocytosis, downregulates Dishevelled-2 protein, and inhibits Wnt3A-stimulated beta-catenin stabilization and LEF/TCF reporter activity in U2OS cells. [3] Niclosamide inhibits the TNF-induced NF-κB reporter activity in a dose- and time-dependent manner in U2OS cells. Niclosamide(125 nM) inhibits NF-κB activation induced by p65, IKKα, IKKβ, IKKγ, and TAK1 in U2OS cells. Niclosamide(< 500 nM) completely block the time- and dose-dependent TNFα-induced alteration of the NF-κB–DNA complex in HL-60 cells. Niclosamide(< 10 nM) inhibits constitutive NF-κB activation in U266 cells. Niclosamide inhibits TNF-induced degradation of IκBα and relocation of p65 in a dose- and time-dependent manner in HL-60, Molm13, or AML primary cells. Niclosamide(500 nM) decreases TNF-induced NF-κB–dependent gene products involved in cell survival in HL-60 cells. Niclosamide dose dependently inhibits the growth and induces robust apoptosis of AML cells associated with decreased Mcl-1 and XIAP levels and increased intracellular ROS levels. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Vero E6 cells MVrGeY5kfGmxbjDhd5NigQ>? NGm5cXVCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBUSVKVIHPvdo9v[X[rcoXzJIlvKF[ncn:gSVYh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJidCC{ZYDsbYNifGmxbjDifUBGVEmVQTygSWM2OD1yLkGg{txO M3TMW|E4PjZ|NUO5
human blood cancer cells M4XzNmN6fG:2b4jpZ:Kh[XO|YYm= MlvkO|IhcA>? NWr0dnQ{S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4h[myxb3SgZ4Fv[2W{IHPlcIx{KGG|c3Xzd4VlKGG|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDd{IHjyd{BjgSCFZXzsJHRqfGW{IFfsc{BCe3OjeTygTWM2OD1yLkGzJO69VQ>? M3rMW|I3Ojd{MEOy
human pancreatic cancer cells NWjvcmxYS3m2b4TvfIlkyqCjc4PhfS=> NG[wTIg4OiCq M1nFbWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJJBidmO{ZXH0bYMh[2GwY3XyJINmdGy|IHHzd4V{e2WmIHHzJIdzd3e2aDDpcohq[mm2aX;uJIFnfGW{IEeyJIhzeyCkeTDD[YxtKFSrdHXyJGdtdyCDc4PhfUwhUUN3ME2wMlE{KM7:TR?= NWfhW4d7OjZ{N{KwN|I>
human ovarian cancer cells Moe2R5l1d3SxeHnjxsBie3OjeR?= NEDtR5Y4OiCq Mor3R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gc5ZiemmjbjDjZY5k\XJiY3XscJMh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPzJiaILzJIJ6KEOnbHygWIl1\XJiR3zvJGF{e2G7LDDJR|UxRTBwMUOg{txO MXGyOlI4OjB|Mh?=
human lung cancer cells M4DWbGN6fG:2b4jpZ:Kh[XO|YYm= NWrHUVZUPzJiaB?= M1n3SmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJIx2dmdiY3HuZ4VzKGOnbHzzJIF{e2W|c3XkJIF{KGe{b4f0bEBqdmirYnn0bY9vKGGodHXyJFczKGi{czDifUBE\WyuIGTpeIVzKEeubzDBd5NigSxiSVO1NF0xNjF|IN88US=> M3jt[|I3Ojd{MEOy
human colon cancer cells MXTDfZRwfG:6aXRCpIF{e2G7 MUK3NkBp MWPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDjc4xwdiClYX7j[ZIh[2WubIOgZZN{\XO|ZXSgZZMh\3Kxd4ToJIlvcGmkaYTpc44h[W[2ZYKgO|IhcHK|IHL5JGNmdGxiVHn0[ZIhT2yxIFHzd4F6NCCLQ{WwQVAvOTNizszN Mn\DNlYzPzJyM{K=
human prostate cancer cells MmrlR5l1d3SxeHnjxsBie3OjeR?= M1jRWVczKGh? NYTqe5ZZS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4heHKxc4TheIUh[2GwY3XyJINmdGy|IHHzd4V{e2WmIHHzJIdzd3e2aDDpcohq[mm2aX;uJIFnfGW{IEeyJIhzeyCkeTDD[YxtKFSrdHXyJGdtdyCDc4PhfUwhUUN3ME2wMlE{KM7:TR?= NV;reppvOjZ{N{KwN|I>
human breast cancer cells NWD2OXBlS3m2b4TvfIlkyqCjc4PhfS=> M3fXZVczKGh? M1rLfWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJIJz\WG|dDDjZY5k\XJiY3XscJMh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPzJiaILzJIJ6KEOnbHygWIl1\XJiR3zvJGF{e2G7LDDJR|UxRTBwMUOg{txO MV2yOlI4OjB|Mh?=
human HeLa cells NXnNU2pkT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUWyOEBp Mkm5TY5pcWKrdHnvckBw\iCVVFHUN{BqdiCqdX3hckBJ\UyjIHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVA:OC5{NTFOwG0> MmDMNlQ6ODB{M{G=
HEK293 cells NVfQeFQyTnWwY4Tpc44h[XO|YYm= NIHEU|g5KGh? Ml\pTY5pcWKrdHnvckBw\iCZboSvZoV1[S2lYYTlcolvKGmwIFjFT|I6OyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJHdvfDODLYP0bY12dGG2ZXSgWG9RTmyjc3igZYN1cX[rdImgZYZ1\XJiODDodpMtKEmFNUC9NE4{PCEQvF2= M2\HeVI3Ojd{MEOy
human PC3 cells NYLGUWRTWHKxbHnm[ZJifGmxbjDhd5NigQ>? Mkm0NVIxKGh? MWjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFCFMzDj[YxteyCjZoTldkAyOjBiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0xNjRizszN MUKxOlY5ODF3OR?=
HEK293 cells NVP6R2wyTnWwY4Tpc44h[XO|YYm= MoXZPEBp M1vXfWlvcGmkaYTpc44hd2ZiV370N2Ew[mW2YT3jZZNmcW5ic3nncoFtcW6pIHnuJGhGUzJ7MzDj[YxteyCjZoTldkA5KGi{czDifUBVV1CobHHzbEBz\XCxcoTldkBie3OjeTygTWM2OD1yLkSg{txO MnTmNlM1PTNyN{O=
human A549 cells NXLlUHlIWHKxbHnm[ZJifGmxbjDhd5NigQ>? MY[xNlAhcA>? M3vDXWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDh[pRmeiBzMkCgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1yLkSg{txO Mn;YNVY3QDBzNUm=
human U87MG cells NUW5NG5bWHKxbHnm[ZJifGmxbjDhd5NigQ>? M{nlblEzOCCq MYPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFV6N13HJINmdGy|IHHmeIVzKDF{MDDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTBwNDFOwG0> MXGxOlY5ODF3OR?=
human HCT116 cells M4TsdWN6fG:2b4jpZ:Kh[XO|YYm= NGXnN2E4OiCq NIKwelZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiN{KgbJJ{KGK7IF3UV{Bie3OjeTygTWM2OD1yLkS1JO69VQ>? NFy5eFMzPjJ5MkCzNi=>
human DU145 cells NE\OcYtRem:uaX\ldoF1cW:wIHHzd4F6 NYjjeItGPzJiaB?= NXTHdnpYSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDEWVE1PSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUCuO{DPxE1? MWOyOFkxODJ|MR?=
human LoVo cells MVXQdo9tcW[ncnH0bY9vKGG|c3H5 NFnjV2oyOjBiaB?= MVTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEyxVn:gZ4VtdHNiYX\0[ZIhOTJyIHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OC55IN88US=> M{fhT|E3PjhyMUW5
human MCF7 cells MXTDfZRwfG:6aXRCpIF{e2G7 MXnDfZRwfG:6aXPpeJkh[WejaX7zeEBGWi2yb4PpeIl3\SCqdX3hckBOS0Z5IHPlcIx{KGK7IF3UV{Bie3OjeTygTWM2OCB;MT6wOkDPxE1? M3fMXVI{PDF4MUmx
human MIAPaCa2 cells NE\1OopRem:uaX\ldoF1cW:wIHHzd4F6 NIrwU24yOjBiaB?= MYrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2LQWDhR4EzKGOnbHzzJIFnfGW{IEGyNEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUGuNUDPxE1? M1zHOFE3PjhyMUW5
MDA-MB-231 cells M4T3SWN6fG:2b4jpZ:Kh[XO|YYm= MlqyO|IhcA>? MkDIR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gSXIhdmWpYYTpeoUhVUSDLV3CMVI{OSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRUKGG|c3H5MEBKSzVyPUCuO|kh|ryP NWHwdlI6OjN2NUm2NVM>
human HeLa cells M2nvN3Bzd2yrZnXyZZRqd25iYYPzZZk> M2KxcVczKGh? Mmf1RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZVzhJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OS52IN88US=> NED1OYwzPDlyMEKzNS=>
human AsPC1 cells NHnMNHBEgXSxdH;4bYPDqGG|c3H5 M{j2SVczKGh? M3r0WWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGF{WENzIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFNiYYPzZZktKEmFNUC9NU41PyEQvF2= NV\rR3NlOjN2NUm2NVM>
human PANC1 cells NVzzeW9{S3m2b4TvfIlkyqCjc4PhfS=> NV7kc2xVPzJiaB?= M13QNmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHBCVkNzIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFNiYYPzZZktKEmFNUC9NU44OyEQvF2= M4PVdVI{PDV7NkGz
human A549 cells  M4j4[XBzd2yrZnXyZZRqd25iYYPzZZk> MWS3NkBp M4\tRmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTNizszN Mn\mNlQ6ODB{M{G=
human HT-29 cells MXTQdo9tcW[ncnH0bY9vKGG|c3H5 M2HndlczKGh? M3XOcGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSGStNlkh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD15LkKg{txO M1zRW|I1QTByMkOx
human A431 cells MnvCVJJwdGmoZYLheIlwdiCjc4PhfS=> NF;H[Xg4OiCq NVjrSWZMSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOFMyKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;OD64JO69VQ>? M3m3TVI1QTByMkOx
human Ava5 cells NXT3SGQ4S3m2b4TvfIlkyqCjc4PhfS=> M4LofVMh\GG7cx?= NWfBfnB7S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSX[jNTDj[YxteyCjZoTldkA{KGSjeYOgZpkhdmW3dILhcEBz\WRiZInlJIF{e2G7LDDDR|UxRTFyIN88US=> MYSyNlA2QTl6Mx?=
human PC3 cells NVT0cmpmWHKxbHnm[ZJifGmxbjDhd5NigQ>? M{LERlczKGh? MYjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFCFMzDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVEyNjdizszN MViyOFkxODJ|MR?=
mouse RAW264.7 cells M1rRNGZ2dmO2aX;uJIF{e2G7 NE\OcogyOCEQvF2= MYDDfZRweHKxdHXjeIl3\SCjY4Tpeol1gSCjZ3HpcpN1KGGwdHjyZZghfG:6aX6gcIV1cGGuIH\hZ5Rwei:ycn;0[YN1cX[nIHHueIlo\W5vaX7keYNm\CClZXzsJIRm[XSqIHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGG2IEGwJJVOKGK7IF3UWEBz\WS3Y4Tpc44h[XO|YYm= MkfuNVk2PDB5NkS=
CHO cells MmXVSpVv[3Srb36gZZN{[Xl? NXnIUFc6S3m2b4Dyc5Rm[3SrdnWgZYN1cX[rdImgZYdicW6|dDDhcpRpemG6IH\1d4lwdiC2b4jpckBHWDV7LXnu[JVk\WRiY3XscEBl\WG2aDDpckBEUE9iY3XscJMh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[nliTWTUJJJm\HWldHnvckBie3OjeR?= NVXPeYQ{OTl3NEC3OlQ>
human HeLa cells Mle4SpVv[3Srb36gZZN{[Xl? MkjmOUDPxE1? NE\4dJozPCCq MVfJcohq[mm2aX;uJI9nKFOWQWSzJIlvKGi3bXHuJGhmVGFiY3XscJMh[XRiNTD1UUBi\nSncjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6 NUD6TYpHOjR7MECyN|E>
human U2OS cells NFvYbndHfW6ldHnvckBie3OjeR?= MkTYNVIvPSEQvF2= MXy2JIg> MXjJcoR2[3Srb36gc4YhcW62ZYLuZYxqgmG2aX;uJI9nKE[{aYr6cIVlOS2JRmCgLJVvc26xd36gc5Jq\2mwKTDlfJBz\XO|ZXSgbY4hcHWvYX6gWVJQWyClZXzsd{BifCBzMj61JJVOKGGodHXyJFYhcHK|IHL5JINwdm[xY3HsJI1q[3Kxc3PvdJk> NWe3UmpsOjN2NUOwO|M>

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
p-STAT3 / STAT3 / c-Myc / Survivin ; 

PubMed: 25970160     

LNCaP, C4-2B or DU145 cells were treated with DMSO, 0.5 μM or 1 μM niclosamide overnight, cells were then stimulated with 10 ng/mL of IL6 for 30 minutes. Whole cell lysates were prepared and subjected to Western blot analysis using antibodies as indicated.


PubMed: 29358661     

KBM5 cells harboring wild-type or T315I-BCR-ABL and K562 cell were exposed to different concentrations of niclosamide, and then analyzed by Western blotting analysis.

p-STAT5 / STAT5 / p-AKT / AKT / p-ERK / ERK ; 

PubMed: 29358661     

CML cells were treated with increasing concentrations of niclosamide for 48 h and subjected to Western blotting analysis. Actin served as a loading control for blots above, which were performed sequentially.


PubMed: 27652012     

Niclosamide decreases intracellular β-catenin levels in A-498 and SW-839 cells. Cells were treated with niclosamide for 24 h prior to WB analysis.

25970160 29358661 27652012
Growth inhibition assay
Cell viability; 

PubMed: 28418862     

Cell viability of colon cancer cells after treatment of niclosamide for a 48-h period. Cell viability was determined by trypan blue exclusion assay from triplicate experiments.

In vivo Niclosamide(40 mg/kg/d, i.p.) inhibits growth of xenografted AML cells in nude mice bearing HL-60 xenograft tumors. [4]


Kinase Assay:


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Protein Kinase profiling assay:

Assay for 22 different proteins kinases is carried out by ProQinase Gmbh. All of the protein kinases are expressed either in Sf9 insect cells or in E.coli as recombinant GST-fusion proteins or His-tagged proteins. Protein kinases are purified by affinity chromatography using either GSH-agarose or Ni_NTH-agarose. A radiometric protein kinase assay is used for measuring the kinase activity of the 22 protein kinases. Briefly, for each protein kinase, 50 μL reaction cocktail containing 60 mM HEPES-NaOH, 3 mM MgCl2, 3 mM MnCl2, 3 μM Na-orthovanadate, 1.2 mM DTT, 50 μg/mL PEG20000, 1 μM [γ-33P]-ATP, Niclosamide, adequate amount of enzyme and its substrate. The PKC-alpha assay additionally contain 1 mM Cacl2, 4 mM EDTA, 5 μg/mL phosphatidylserine and 1 μg/mL 1, 2-Dioleyl-glycerol. The reaction cocktails are incubated at 37 °C for 60 minutes and stop with 50 μL 2% (v/v) H3PO4. Incorporation of 33Pi is determined with a microplate scintillation counter. The activities and the IC50 values are calculated using Quattro Workflow V2.28.
Cell Research:


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  • Cell lines: Hela, A549, Du145, HT-29, A431, PC3 cells
  • Concentrations: 16 μM
  • Incubation Time: 72 hours
  • Method:

    Cells are plated in 96-well culture plates with cell density of 3-4 × 103 cells/well and treat with Niclosamide by adding 100 μL medium containing Niclosamide of various concentrations on the second day. After 72-hour's treatment, MTT is added to each well and incubated for additional 4-5 hours, and the absorbance is measured on a microplate reader at 570nm. Cell growth inhibition is evaluated as the ratio of the absorbance of the sample to that of the control. The results are representative of at least 3 independent experiments.

    (Only for Reference)
Animal Research:


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  • Animal Models: nude mice bearing HL-60 xenograft tumors.
  • Formulation: DMSO
  • Dosages: 40 mg/kg
  • Administration: Intraperitoneal injection
    (Only for Reference)

Solubility (25°C)

In vitro DMF 12 mg/mL warmed (36.68 mM)
DMSO Insoluble
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
1% DMSO+30% polyethylene glycol+1% Tween 80
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 327.12


CAS No. 50-65-7
Storage powder
in solvent
Synonyms Niclocide
Smiles OC1=CC=C(Cl)C=C1C(=O)NC2=C(Cl)C=C(C=C2)[N+]([O-])=O

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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