Niclosamide (BAY2353)

Catalog No.S3030 Synonyms: Niclocide, NSC 178296

For research use only.

Niclosamide (BAY2353, Niclocide, NSC 178296) can inhibit DNA replication and inhibit STAT3 with IC50 of 0.7 μM in a cell-free assay. Niclosamide selectively inhibited the phosphorylation of STAT3 and had no obvious inhibition against the activation of other homologues (e.g., STAT1 and STAT5).

Niclosamide (BAY2353) Chemical Structure

CAS No. 50-65-7

Selleck's Niclosamide (BAY2353) has been cited by 31 publications

Purity & Quality Control

Choose Selective STAT Inhibitors

Other STAT Products

Biological Activity

Description Niclosamide (BAY2353, Niclocide, NSC 178296) can inhibit DNA replication and inhibit STAT3 with IC50 of 0.7 μM in a cell-free assay. Niclosamide selectively inhibited the phosphorylation of STAT3 and had no obvious inhibition against the activation of other homologues (e.g., STAT1 and STAT5).
Targets
STAT3 [1]
(in Hela cells)
0.7 μM
In vitro

Niclosamide (< 5 μM) dose dependently inhibits STAT3-mediated luciferase reporter activity with IC50 of 0.25 μM in HeLa cells. Niclosamide(< 2 μM) dose dependently inhibits the phosphorylation of STAT3 in Du145 cells. Niclosamide (1 μM) inhibits the EGF-induced nuclear translocation of STAT3 in Du145 cells. Niclosamide(< 2 μM) dose dependently inhibits the transcription of STAT3 downstream genes in Du145 cells. Niclosamide(< 10 μM) dose dependently induces G0/G1 arrest and apoptosis of Du145 cancer cells. [1] Niclosamide is able to inhibit SARS-CoV replication at a micromolar concentration in Vero E6 cells infected with SARS-CoV. [2] Niclosamide(< 7.5 μM) promotes Frizzled1 endocytosis, downregulates Dishevelled-2 protein, and inhibits Wnt3A-stimulated beta-catenin stabilization and LEF/TCF reporter activity in U2OS cells. [3] Niclosamide inhibits the TNF-induced NF-κB reporter activity in a dose- and time-dependent manner in U2OS cells. Niclosamide(125 nM) inhibits NF-κB activation induced by p65, IKKα, IKKβ, IKKγ, and TAK1 in U2OS cells. Niclosamide(< 500 nM) completely block the time- and dose-dependent TNFα-induced alteration of the NF-κB–DNA complex in HL-60 cells. Niclosamide(< 10 nM) inhibits constitutive NF-κB activation in U266 cells. Niclosamide inhibits TNF-induced degradation of IκBα and relocation of p65 in a dose- and time-dependent manner in HL-60, Molm13, or AML primary cells. Niclosamide(500 nM) decreases TNF-induced NF-κB–dependent gene products involved in cell survival in HL-60 cells. Niclosamide dose dependently inhibits the growth and induces robust apoptosis of AML cells associated with decreased Mcl-1 and XIAP levels and increased intracellular ROS levels. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
PC3 MVHBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M4PZW|EzOCCqcoO= M2fiXGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUFOzJINmdGy|IHHmeIVzKDF{MDDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTBwNN88US=> M4T0Z|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NkiwNVU6Lz5zNk[4NFE2QTxxYU6=
A549 NEOwU21CdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M4P1e|EzOCCqcoO= NYDYdHRtSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEGyNEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUCuOO69VQ>? MmO3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
U87MG NI\NOZdCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NUS2fYtCOTJyIHjydy=> NUDsS5VZSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDVPFdOTyClZXzsd{Bi\nSncjCxNlAhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlTPxE1? M2TONlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NkiwNVU6Lz5zNk[4NFE2QTxxYU6=
LoVo NXrSXIh1SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M4ntcFEzOCCqcoO= M2XiVmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTH;Wc{Bk\WyuczDh[pRmeiBzMkCgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1yLkhOwG0> MnHsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
MIAPaCa2 MV;BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NF7UOlAyOjBiaILz MXjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2LQWDhR4Ez[2WubIOgZYZ1\XJiMUKwJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MT6x{txO MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjZ6MEG1PUc,OTZ4OECxOVk9N2F-
PC3 NFrnRoJHfW6ldHnvckBie3OjeR?= NY\qdpdIOSCqch?= MojQTY5pcWKrdHnvckBw\iCvaYTvZ4hwdmS{aXHsJI1mdWK{YX7lJJBwfGWwdHnhcEBqdiCqdX3hckBRSzNiY3XscJMh[W[2ZYKgNUBpeg>? MnLmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
HEK293 MWnGeY5kfGmxbjDhd5NigQ>? M4nCWlEhcHJ? M4rSOWlvcGmkaYTpc44hd2ZibXn0c4Npd26mcnnhcEBu\W2kcnHu[UBxd3SnboTpZYwhcW5iaIXtZY4hUEWNMkmzJINmdGy|IHHmeIVzKDFiaIK= Mlj3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
neural precursor NVzpOIhLTnWwY4Tpc44h[XO|YYm= M1XHTWlvcGmkaYTpc44hd2ZibnX1do9{eGincnWgdJJwdGmoZYLheIlwdiCxZjDtc5V{\SCwZYXyZYwheHKnY4Xyd49zKGOnbHzzJIJ6KE2WVDDhd5NigQ>? M1jwb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEG3OlMyLz5zN{SxO|Y{OTxxYU6=
Vero E6 MmfqRY51cX[rcnHsJIF{e2G7 NX[1XJk1SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhW0GUUzDjc5JwdmG4aYL1d{BqdiCYZYLvJGU3KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolz[WxicnXwcIlk[XSrb36gZpkhTUyLU1GsJGVEPTB:MD6x{txO MknVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4NkO1N|koRjF5Nk[zOVM6RC:jPh?=
Vero E6 M4fadWN6fG:2b4jpZ4l1gSCjc4PhfS=> MnP0R5l1d3SxeHnjbZR6KGGpYXnud5QhXmW{bzDFOkBk\WyuczDifUBOXFRiYYPzZZktKEOFNUC9NlIvOc7:TR?= Ml25QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4NkO1N|koRjF5Nk[zOVM6RC:jPh?=
RAW264.7 MUPDfZRweHKxdHXjeIl3\SCjc4PhfS=> MYixNEB2VQ>? Ml3iR5l1d3C{b4TlZ5RqfmViYXP0bZZqfHliYXfhbY5{fCCjboTodoF5KHSxeHnuJIxmfGijbDDmZYN1d3JxcILveIVkfGm4ZTDhcpRq\2WwLXnu[JVk\WRiY3XscEBl\WG2aDDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjdDCxNEB2VSCkeTDNWHQhemWmdXP0bY9vKGG|c3H5 MkP2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl3NEC3OlQoRjF7NUSwO|Y1RC:jPh?=
CHO MX7DfZRweHKxdHXjeIl3\SCjc4PhfS=> MkHwR5l1d3C{b4TlZ5RqfmViYXP0bZZqfHliYXfhbY5{fCCjboTodoF5KG[3c3nvckB1d3irbjDGVFU6NWmwZIXj[YQh[2WubDDk[YF1cCCrbjDDTG8h[2WubIOgZZN{\XO|ZXSgZZMh[2WubDD2bYFjcWyrdImgZpkhVVSWIILl[JVkfGmxbjDhd5NigQ>? MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTV2MEe2OEc,OTl3NEC3OlQ9N2F-
CHO NVHMXZlvTnWwY4Tpc44h[XO|YYm= NEfzbpRKdmirYnn0bY9vKG:oIFLhZ4ltdHW|IHHueIhz[WOrczDhcpRpemG6IIDyc5Rm[3SrdnWgZY51cWenbjDo[ZB1[W2ncjDwdoUueG:{ZTD0c{Bxd3KnIHPvcpZmenOrb36gbY4hS02JMj3lfJBz\XO|aX7nJGNJVyClZXzsdy=> NYHn[nZrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1OFA4PjRpPkG5OVQxPzZ2PD;hQi=>
Ava5 NV7s[pdVSW62aY\pdoFtKGG|c3H5 MYCzJIRigXN? NYTFc5M5SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEOYIHflco91gXCnIEHiJIlv\mWldHXkJIlvKEG4YUWgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC4aYLhcEBz\XCuaXPheIlwdiCjZoTldkA{KGSjeYOgZpkh[myxdDDofYJzcWSrenH0bY9vKGGwYXz5d4l{NCCHQ{WwQVAvOTcQvF2= NYr1U5JYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwOVk6QDNpPkKyNFU6QTh|PD;hQi=>
Ava5 M3vuS2FvfGm4aYLhcEBie3OjeR?= MXSzJIRigXN? NEfKRXdCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJS1ZiZ3Xuc5R6eGViMXKgbY5n\WO2ZXSgbY4hSX[jNTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJJJmeGyrY3H0bY9vKGGodHXyJFMh\GG7czDifUBjdG:2IHj5ZpJq\Gm8YYTpc44h[W6jbInzbZMtKEWFOUC9NE44|ryP M3Hxe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEW5PVg{Lz5{MkC1PVk5OzxxYU6=
Ava5 MXXDfZRwfG:6aXPpeJkh[XO|YYm= NWPVO4RHOyCmYYnz M4TXTmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGF3[TViY3XscJMh[W[2ZYKgN{Bl[Xm|IHL5JI5mfXS{YXygdoVlKGS7ZTDhd5NigSxiQ1O1NF0yOM7:TR?= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB3OUm4N{c,OjJyNUm5PFM9N2F-
HFF MV;BcpRq[XCrY3;tdIxmgGGwIHHzd4F6 MmrDNlQhcHK| MorqRY51cWGyaXPvcZBt\XijbjDhZ5Rqfmm2eTDh[4FqdnO2IGTvfI9xdGG|bXGg[49v\GmrIGLIJJRi[2i7en;peIV{KGmwZnXjeIVlKGmwIFjGSkBk\WyuczDhd5Nme3OnZDDhd{BcO0ifLYXyZYNqdCCrbnPvdpBwemG2aX;uJIFnfGW{IEK0JIhzeyCkeTDzZ4lvfGmubHH0bY9vKGy3bXnu[ZNk\W6lZTDjc5VvfGW{LDDNTWM6OD1yLkNOwG0> MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl5MEmzO{c,OjJ7N{C5N|c9N2F-
HFF NIHySodCdnSrYYDpZ49ueGyneHHuJIF{e2G7 MXuyOEBpenN? MlTkRY51cWGyaXPvcZBt\XijbjDhZ5Rqfmm2eTDh[4FqdnO2IGTvfI9xdGG|bXGg[49v\GmrIGLIJJRi[2i7en;peIV{KGmwZnXjeIVlKGmwIFjGSkBk\WyuczDhd5Nme3OnZDDhd{BcO0ifLYXyZYNqdCCrbnPvdpBwemG2aX;uJIFnfGW{IEK0JIhzeyCkeTDzZ4lvfGmubHH0bY9vKGy3bXnu[ZNk\W6lZTDjc5VvfGW{LDDNTWM2OD1yLkNOwG0> NHT2XVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mkm3NFk{Pyd-MkK5O|A6Ozd:L3G+
MDA-MB-231 NV24bI17S3m2b4TvfIlkcXS7IHHzd4F6 MYfDXZRwfG:6aXPpeJkh[WejaX7zeEBGWi2wZXfheIl3\SCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIJ6KE2WUzDhd5NigSxiSVO1NF0xNjd7zszN MoDuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2MU[xPVEoRjJ|NEG2NVkyRC:jPh?=
MCF7 M{fVWmN6fG:2b4jpZ4l1gSCjc4PhfS=> NUK5PFBqS1m2b4TvfIlkcXS7IHHnZYlve3RiRWKtdI9{cXSrdnWgbJVu[W5iTVPGO{Bk\WyuczDifUBOXFNiYYPzZZktKEmFNUC9NU4xPs7:TR?= NEL2V3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SxOlE6OSd-MkO0NVYyQTF:L3G+
AsPC1 Mn\ZR5l1d3SxeHnjbZR6KGG|c3H5 NVOzfpI5S1m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSXOSQ{GgZ4VtdHNiYomgUXRUKGG|c3H5MEBKSzVyPUGuOFfPxE1? NUXu[nBkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NVYyQTFpPkKzOFE3OTlzPD;hQi=>
PANC1 NG\SOYxEgXSxdH;4bYNqfHliYYPzZZk> MYXDXZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDQRW5EOSClZXzsd{BjgSCPVGOgZZN{[XluIFnDOVA:OS55M988US=> NW\RW|JZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NVYyQTFpPkKzOFE3OTlzPD;hQi=>
HEK293 MmTTSpVv[3Srb36gZZN{[Xl? MmfuPEBpenN? MoHwTY5pcWKrdHnvckBw\iCZboSzRU9j\XSjLXPhd4VqdiC|aXfuZYxqdmdiaX6gTGVMOjl|IHPlcIx{KGGodHXyJFghcHK|IHL5JHRQWG[uYYPoJJJmeG:{dHXyJIF{e2G7LDDJR|UxRTBwNN88US=> MkTOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NUOwO|MoRjJ|NEWzNFc{RC:jPh?=
U2OS NGe3OGNHfW6ldHnvckBie3OjeR?= NHLuOXoyOi53IIXN NGO2bZY3KGi{cx?= MnzLTY5lfWO2aX;uJI9nKGmwdHXycoFtcXqjdHnvckBw\iCIcnn6foxm\DFvR1\QJEh2dmuwb4fuJI9zcWerbjmg[ZhxemW|c3XkJIlvKGi3bXHuJHUzV1NiY3XscJMh[XRiMUKuOUB2VSCjZoTldkA3KGi{czDifUBkd26ob3PhcEBucWO{b4Pjc5B6 MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR3M{C3N{c,OjN2NUOwO|M9N2F-
MDA-MB-231 NWDNSFZ7S3m2b4TvfIlkcXS7IHHzd4F6 Mor0O|IhcHK| M3THRWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGVTKG6nZ3H0bZZmKE2GQT3NRk0zOzFiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VWyCjc4PhfUwhUUN3ME2wMlc6|ryP MorLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NUm2NVMoRjJ|NEW5OlE{RC:jPh?=
MCF7 Mk\KR5l1d3SxeHnjbZR6KGG|c3H5 NEHn[Zk4OiCqcoO= MXfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDFVkBxd3OrdHn2[UBOS0Z5IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFNiYYPzZZktKEmFNUC9NU4xPs7:TR?= NWK4dlBIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVk3OTNpPkKzOFU6PjF|PD;hQi=>
AsPC1 M{\aN2N6fG:2b4jpZ4l1gSCjc4PhfS=> NILCZ484OiCqcoO= MoG3R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRZNRSzFiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VWyCjc4PhfUwhUUN3ME2xMlQ4|ryP MmqwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NUm2NVMoRjJ|NEW5OlE{RC:jPh?=
PANC1 NF3VPIlEgXSxdH;4bYNqfHliYYPzZZk> Mm[2O|IhcHK| MlTTR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVGFPSzFiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VWyCjc4PhfUwhUUN3ME2xMlc{|ryP M1fSb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEW5OlE{Lz5{M{S1PVYyOzxxYU6=
MDA-MB-231 MYTBcpRqfHWvb4KgZZN{[Xl? M37YUFc2KG2pL3vn MWq1JIRigXN? Mli4RY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iRWKgcoVo[XSrdnWgUWRCNU2ELUKzNUBk\WyuczD4[Y5w\3KjZoTl[EBqdiCwdXTlJI1wfXOnIHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidIXtc5Ih\3Kxd4ToJIF1KDd3IH3nM4toNCCybzDx[EBnd3JiNTDkZZl{ M{jmXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEW5OlE{Lz5{M{S1PVYyOzxxYU6=
MDA-MB-231 M3S2RWFvfGm2dX3vdkBie3OjeR?= Mmj0NVIvPSCvZz;r[y=> NHPoUoQ2KGSjeYO= M2HsNmFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFXSJI5m\2G2aY\lJG1FSS2PQj2yN|Eh[2WubIOgfIVvd2e{YX\0[YQhcW5iboXk[UBud3W|ZTDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIIT1cY9zKGe{b4f0bEBifCBzMj61JI1oN2upLDDpdEBy\CCob4KgOUBl[Xm| MmC5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NUm2NVMoRjJ|NEW5OlE{RC:jPh?=
MDA-MB-231 Mn\rRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NGnGb2EyOCC2bzCyNEB2VQ>? NFz4e|UzPCCqcoO= MYfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEWUIH7l[4F1cX[nIF3ERU1OSi1{M{GgZ4VtdHNiYYSgNVAhfG9iMkCgeW0h[W[2ZYKgNlQhcHK|IHL5JG1VXCCjc4PhfS=> NUW5N2k6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVk3OTNpPkKzOFU6PjF|PD;hQi=>
MDA-MB-231 M3PUZWZ2dmO2aX;uJIF{e2G7 M{jEOVI1KGi{cx?= NF36dpVKdmirYnn0bY9vKG:oIGPURXQ{KHC{b33veIVzKGGldHn2bZR6KGmwIHj1cYFvKEWUIH7l[4F1cX[nIF3ERU1OSi1{M{GgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KGS3YXygcJVkcW[ncnHz[UBz\XCxcoTldkBie3OjeR?= NEXmU2k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S1PVYyOyd-MkO0OVk3OTN:L3G+
MDA-MB-231 NID2NIdHfW6ldHnvckBie3OjeR?= NF\yfWwzOCC3TR?= M2X3PFI1KGi{cx?= M{jTZWlvcGmkaYTpc44hd2ZiU2TBWFMheHKxbX;0[ZIh[WO2aY\peJkhcW5iaIXtZY4hTVJibnXnZZRqfmViTVTBMW1DNTJ|MTDj[YxteyCjdDCyNEB2VSCjZoTldkAzPCCqcoOgZpkh\HWjbDDseYNq\mW{YYPlJJJmeG:{dHXyJIF{e2G7 M3i3O|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEW5OlE{Lz5{M{S1PVYyOzxxYU6=
MDA-MB-231 NXzQUWFUTnWwY4Tpc44h[XO|YYm= NVHtfoNEOTBidV2= NVjIc5F4OjRiaILz NIHq[4RKdmirYnn0bY9vKG:oIGPURXQ{KHC{b33veIVzKGGldHn2bZR6KGmwIHj1cYFvKEWUIH7l[4F1cX[nIF3ERU1OSi1{M{GgZ4VtdHNiYYSgNVAhfU1iYX\0[ZIhOjRiaILzJIJ6KGS3YXygcJVkcW[ncnHz[UBz\XCxcoTldkBie3OjeR?= MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR3OU[xN{c,OjN2NUm2NVM9N2F-
MDA-MB-231 MorGSpVv[3Srb36gZZN{[Xl? MmL5NUB1dyBzMDD1US=> NYH3ZZBPOjRiaILz NXnhNYl6UW6qaXLpeIlwdiCxZjDTWGFVOyCrbjDoeY1idiCHUjDu[YdifGm4ZTDNSGEuVUJvMkOxJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBw\iC2b4ThcEBUXEGWMzDs[ZZmdCCjdDCxJJRwKDFyIIXNJIFnfGW{IEK0JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> NHH6N|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S1PVYyOyd-MkO0OVk3OTN:L3G+
MDA-MB-231 NGPwWolHfW6ldHnvckBie3OjeR?= M{Toe|EhfG9iMUCgeW0> MoDJOFghcHK| NFz5PJBKdmS3Y4Tpc44hd2ZibX;ydIhwdG:paXPhcEBkcGGwZ3XzJIlvKGi3bXHuJGVTKG6nZ3H0bZZmKE2GQT3NRk0zOzFiY3XscJMh[XRiMTD0c{AyOCC3TTDh[pRmeiB2ODDodpMh[nlibHnnbJQhdWmlcn;zZ49xcWNiYX7hcJl{cXN? NH7WS|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S1PVYyOyd-MkO0OVk3OTN:L3G+
MDA-MB-231 MljBSpVv[3Srb36gZZN{[Xl? Mke5NUB1dyBzMDD1US=> NXr1boxsPDhiaILz NVyweZhZUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDFVkBv\WejdHn2[UBOTEFvTVKtNlMyKGOnbHzzJIF1KDFidH:gNVAhfU1iYX\0[ZIhPDhiaILzJIJ6KGyrZ3j0JI1q[3Kxc3PvdIlkKGGwYXz5d4l{ NH7semo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S1PVYyOyd-MkO0OVk3OTN:L3G+
MDA-MB-231 MVHBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MonjNUB1dyBzMDD1US=> NWTRbYFuPDhiaILz NUXq[5JRSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDFVkBv\WejdHn2[UBOTEFvTVKtNlMyKGOnbHzzJIF1KDFidH:gNVAhfU1iYX\0[ZIhPDhiaILzJIJ6KGyrZ3j0JI1q[3Kxc3PvdIlkKGGwYXz5d4l{ Ml3oQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NUm2NVMoRjJ|NEW5OlE{RC:jPh?=
MDA-MB-231 NHrGVldHfW6ldHnvckBie3OjeR?= M2HQVlEhfG9iMUCgeW0> NG\Xb2gzPCCqcoO= M{HweWlvcGmkaYTpc44hd2ZiU2TBWFMhcW5iaIXtZY4hTVJibnXnZZRqfmViTVTBMW1DNTJ|MTDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gc4YheGixc4Doc5J6dGG2ZXSgV3RCXDNiYYSgWJlzNTdyNTDs[ZZmdCCjdDCxJJRwKDFyIIXNJIFnfGW{IEK0JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR3OU[xN{c,OjN2NUm2NVM9N2F-
HeLa NUPmOIZPTnWwY4Tpc44h[XO|YYm= MWmyOEBpenN? NUnRRnBtUW6qaXLpeIlwdiCxZjDTWGFVOyCrbjDoeY1idiCKZVzhJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGlEPTB;MD6yOe69VQ>? NFHqTZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFI{OSd-MkS5NFAzOzF:L3G+
DU145 NEf5XoJCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MnLaO|IhcHK| MWLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKESXMUS1JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OC55zszN M1P0UVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwNlMyLz5{NEmwNFI{OTxxYU6=
DU145 MXjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1XtN|EyKHSxIEGyJIRigXN? M2LVVGdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGRWOTR3IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yh[2:ub375JIZwem2jdHnvckBi\nSncjCxNUB1dyBzMjDkZZl{KGK7IHPyfZN1[WxidnnvcIV1KHO2YXnubY5oKGKjc3XkJI1q[3Kxc3PvdIlkKGGwYXz5d4l{NCCLQ{WwQVAvP87:TR?= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEKzNUc,OjR7MECyN|E9N2F-
HeLa MnfFRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NU\xfpB3PzJiaILz NUHpU5lWSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MT60{txO MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEKzNUc,OjR7MECyN|E9N2F-
A549 NFHFUItCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M4rzXlczKGi{cx?= Ml7zRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDNUS5JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:O87:TR?= MnqzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MECyN|EoRjJ2OUCwNlMyRC:jPh?=
HT-29 NWXCZZVxSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NXPiPVd2PzJiaILz M3TLNWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSGStNlkh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD15LkNOwG0> NHjhdGE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFI{OSd-MkS5NFAzOzF:L3G+
A431 NGS5[YdCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MVK3NkBpenN? MnOwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDNEOxJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:QC56zszN MkO1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MECyN|EoRjJ2OUCwNlMyRC:jPh?=
PC3 MkD5RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MoO0O|IhcHK| MVHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFCFMzDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVEyNjgQvF2= MkPLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MECyN|EoRjJ2OUCwNlMyRC:jPh?=
HeLa NGfDcHJHfW6ldHnvckBie3OjeR?= M4PMOFUhfU1? MV6yOEBpenN? MYrJcohq[mm2aX;uJI9nKFOWQWSzJIlvKGi3bXHuJGhmVGFiY3XscJMh[XRiNTD1UUBi\nSncjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6 NHHkZ5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFI{OSd-MkS5NFAzOzF:L3G+
DU145 M2PFVGZ2dmO2aX;uJIF{e2G7 MUixJJVO NV\QbXlPOiCqcoO= NHvITJpKdmirYnn0bY9vKG:oIGPURXQ{KG63Y3zlZZIhfHKjboPsc4NifGmxbjDpckBGT0Zic4TpcZVt[XSnZDDoeY1idiCGVUG0OUBk\WyuczDheEAyKHWPIHHkcYlvcXO2ZYLl[EBj\W[xcnWgNVAxKG6pL33sJGVITiC|dHnteYxifGmxbjDt[YF{fXKnZDDh[pRmeiB{IHjyd{BjgSClb37mc4NidCCuYYPldkBucWO{b4Pjc5B6 NYXEUlh6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFAzOzFpPkK0PVAxOjNzPD;hQi=>
DU145 M1PBSWZ2dmO2aX;uJIF{e2G7 M4C0VFA2KHSxIEGwJJVO M3\6XlIhcHK| NX\SUoxzUW6qaXLpeIlwdiCxZjDTWGFVOyCrboTldoFkfGmxbjD3bZRpKESQQTDibY5lcW6pIIPpeIUhcW5iaIXtZY4hTFVzNEWgZ4VtdHNiYYSgNFUhfG9iMUCgeW0h[W[2ZYKgNkBpenNiYomgSW1USQ>? NWD6PGJNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFAzOzFpPkK0PVAxOjNzPD;hQi=>
human MVPGeY5kfGmxbjDhd5NigQ>? MmPFNE4yKHSxIEKgeW0> Mnq0NkBpenN? NHrOVIdF\WO{ZXHz[UBqdiCleXPsbY4hTDFicILveIVqdiCuZY\lcEBqdiCqdX3hckBFXTF2NTDj[YxteyCjdDCwMlEhfG9iMjD1UUBi\nSncjCyJIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> NFm5OFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFI{OSd-MkS5NFAzOzF:L3G+
human MX;GeY5kfGmxbjDhd5NigQ>? M{PjTlAvOSC2bzCyJJVO MX6yJIhzew>? M3nCWGRm[3KnYYPlJIlvKGNvTYnjJJBzd3SnaX6gcIV3\WxiaX6gbJVu[W5iRGWxOFUh[2WubIOgZZQhOC5zIITvJFIhfU1iYX\0[ZIhOiCqcoOgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| NIHBZ5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFI{OSd-MkS5NFAzOzF:L3G+
human NE[xR5ZHfW6ldHnvckBie3OjeR?= M1O4[|AvOSC2bzCyJJVO M4fPRVIhcHK| MXvE[YNz\WG|ZTDpckBD[2xvWHygdJJwfGWrbjDs[ZZmdCCrbjDoeY1idiCGVUG0OUBk\WyuczDheEAxNjFidH:gNkB2VSCjZoTldkAzKGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? NHfjWWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFI{OSd-MkS5NFAzOzF:L3G+
DU145 MUjGeY5kfGmxbjDhd5NigQ>? NYn1NHZiOC5zIITvJFIhfU1? Mn7YNkBpenN? NETZRmRFd3ewcnXneYxifGmxbjDv[kBO[2xvMTDwdo91\WmwIHnuJIh2dWGwIFTVNVQ2KGOnbHzzJIF1KDBwMTD0c{AzKHWPIHHmeIVzKDJiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEKzNUc,OjR7MECyN|E9N2F-
human NGT3cXhHfW6ldHnvckBie3OjeR?= NWjP[oxPOiC3TR?= M2rzNVIhcHK| MkHvTY5pcWKrdHnvckBw\iCVVFHUN{BxcG:|cHjvdplt[XSrb36gZZQhXHm{N{C1JIlvKGi3bXHuJGRWOTR3IHPlcIx{KGG2IEKgeW0hf2m2aHnuJFIhcHK|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? NGfteVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFI{OSd-MkS5NFAzOzF:L3G+
breast cancer cells M2nqUmN6fG:2b4jpZ4l1gSCjc4PhfS=> MkPPO|IhcHK| MkTHR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gZpJm[XO2IHPhcoNmeiClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA4OiCqcoOgZpkhS2WubDDUbZRmeiCJbH:gRZN{[XluIFnDOVA:OC5zM988US=> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ5MkCzNkc,OjZ{N{KwN|I9N2F-
colon cancer cells MXHDfZRwfG:6aXPpeJkh[XO|YYm= MUC3NkBpenN? NXTqbIxbS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4h[2:ub36gZ4Fv[2W{IHPlcIx{KGG|c3Xzd4VlKGG|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDd{IHjyd{BjgSCFZXzsJHRqfGW{IFfsc{BCe3OjeTygTWM2OD1yLkGz{txO M4H2WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MkeyNFMzLz5{NkK3NlA{OjxxYU6=
lung cancer cells MlnzR5l1d3SxeHnjbZR6KGG|c3H5 MoXEO|IhcHK| M4HQS2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJIx2dmdiY3HuZ4VzKGOnbHzzJIF{e2W|c3XkJIF{KGe{b4f0bEBqdmirYnn0bY9vKGGodHXyJFczKGi{czDifUBE\WyuIGTpeIVzKEeubzDBd5NigSxiSVO1NF0xNjF|zszN MonqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{N{KwN|IoRjJ4MkeyNFMzRC:jPh?=
prostate cancer cells MnO2R5l1d3SxeHnjbZR6KGG|c3H5 M1rDe|czKGi{cx?= NWHENI5YS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4heHKxc4TheIUh[2GwY3XyJINmdGy|IHHzd4V{e2WmIHHzJIdzd3e2aDDpcohq[mm2aX;uJIFnfGW{IEeyJIhzeyCkeTDD[YxtKFSrdHXyJGdtdyCDc4PhfUwhUUN3ME2wMlE{|ryP NUTXb3JoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[yO|IxOzJpPkK2NlczODN{PD;hQi=>
ovarian cancer cells MVHDfZRwfG:6aXPpeJkh[XO|YYm= NUHhZ2ZVPzJiaILz M3ux[mN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJI93[XKrYX6gZ4Fv[2W{IHPlcIx{KGG|c3Xzd4VlKGG|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDd{IHjyd{BjgSCFZXzsJHRqfGW{IFfsc{BCe3OjeTygTWM2OD1yLkGz{txO MnX6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{N{KwN|IoRjJ4MkeyNFMzRC:jPh?=
blood cancer cells M{K4XmN6fG:2b4jpZ4l1gSCjc4PhfS=> NFPCZ2Y4OiCqcoO= MV\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDicI9w\CClYX7j[ZIh[2WubIOgZZN{\XO|ZXSgZZMh\3Kxd4ToJIlvcGmkaYTpc44h[W[2ZYKgO|IhcHK|IHL5JGNmdGxiVHn0[ZIhT2yxIFHzd4F6NCCLQ{WwQVAvOTQQvF2= M4DaOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MkeyNFMzLz5{NkK3NlA{OjxxYU6=
pancreatic cancer cells MXfDfZRwfG:6aXPpeJkh[XO|YYm= M2DkUlczKGi{cx?= NYr0fodPS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4heGGwY4LlZZRq[yClYX7j[ZIh[2WubIOgZZN{\XO|ZXSgZZMh\3Kxd4ToJIlvcGmkaYTpc44h[W[2ZYKgO|IhcHK|IHL5JGNmdGxiVHn0[ZIhT2yxIFHzd4F6NCCLQ{WwQVAvOTQQvF2= M4PUXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MkeyNFMzLz5{NkK3NlA{OjxxYU6=
HEK293 Mn\DSpVv[3Srb36gZZN{[Xl? MYi4JIhzew>? NW\QdFRbUW6qaXLpeIlwdiCxZjDXcpQw[mW2YT3jZZRmdmmwIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKFewdEPBMZN1cW23bHH0[YQhXE:SRnzhd4gh[WO2aY\peJkh[W[2ZYKgPEBpenNuIFnDOVA:OC5|NN88US=> NFTCdVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkK3NlA{Oid-Mk[yO|IxOzJ:L3G+
HCT116 NXjIVXRbS3m2b4TvfIlkcXS7IHHzd4F6 MUG3NkBpenN? M3LyUGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDFzNjDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB5MjDodpMh[nliTWTTJIF{e2G7LDDJR|UxRTBwNEZOwG0> M3PP[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MkeyNFMzLz5{NkK3NlA{OjxxYU6=
U2OS M4K0PGZ2dmO2aX;uJIF{e2G7 NGHS[VIyOi53IIXN MYW2JIhzew>? NF;qb4pKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5iaX6gbJVu[W5iVULPV{Bk\WyuczDhd5Nme3OnZDDhd{BqdnSncn7hcIl7[XSrb36gc4Yh\nKreors[YQuT0[SIHH0JFEzNjVidV2gZYZ1\XJiNjDodpMh[nliY3;u[o9k[WxibXnjdo9{[2:yaXOgZY5idHm|aYO= NFXMb449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkK3NlA{Oid-Mk[yO|IxOzJ:L3G+
HCT116 NYrhRlhsTnWwY4Tpc44h[XO|YYm= NU\4cHdIOyCqcoO= NVTqeoZmTW[oZXP0JI9vKEGWUDDoc41md3O2YYPpd{BqdiCqdX3hckBJS1RzMU[gZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKEGWUDDs[ZZmdCCjZoTldkA{KGi{czDpckBi[nOnbnPlJI9nKGeudXPvd4Uh[nliQWTQJIJqd2y3bXnu[ZNk\W62IHHzd4F6NCCHQ{WwQVAvODV4zszN MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDJ|M{[4NEc,Ojh{M{O2PFA9N2F-
HEK293 M4OwNGZ2dmO2aX;uJIF{e2G7 M3nsXVYhcHK| M2HpbWlvcGmkaYTpc44hd2ZiV370M4JmfGFvY3H0[Y5qdiC|aXfuZYxqdmdiKIXub45wf25ib4Lp[4lvMSCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhX262M1Gtd5RqdXWuYYTl[EBj\XSjLXPheIVvcW5icnXzdI9ve2VidILhcpNkemmydHnvckBi\nSncjC2JIhzeyCkeTDUU3BndGG|aDDkeYFtKGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OD1yLkGy{txO MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDJ|M{[4NEc,Ojh{M{O2PFA9N2F-
HCT116 MofWSpVv[3Srb36gZZN{[Xl? NX\vV2k3OyCqcoO= M{fx[2Vn\mWldDDvckBCXFBiaH;t[Y9{fGG|aYOgbY4hcHWvYX6gTGNVOTF4IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBCXFBibHX2[Ywh[W[2ZYKgN{BpenNiaX6gZYJ{\W6lZTDv[kBodHWlb4PlJIJ6KEGWUDDibY9tfW2rbnXzZ4VvfCCjc4PhfUwhUUN3ME2wMlI2|ryP MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDJ|M{[4NEc,Ojh{M{O2PFA9N2F-
HCT116 MWrGeY5kfGmxbjDhd5NigQ>? MUm1JJVO M2H5U|E5KGi{cx?= M1ftT2lvcGmkaYTpc44hd2ZiV370M4JmfGFvY3H0[Y5qdiC|aXfuZYxqdmdiaX6gbJVu[W5iSFPUNVE3KGOnbHzzJIF{e2W|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKGO7dH;zc4xq[yCkZYThMYNifGWwaX6gdJJwfGWrbjDlfJBz\XO|aX;uJIF1KDVidV2gZYZ1\XJiMUigbJJ{KGK7IGfld5Rmem5iYnzveEBu\XSqb3S= NWHTT|l7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiyN|M3QDBpPkK4NlM{PjhyPD;hQi=>
HCT116 MUTGeY5kfGmxbjDhd5NigQ>? MUi1JJVO Mn7vNVghcHK| MlTZTY5pcWKrdHnvckBw\iCZboSvZoV1[S2lYYTlcolvKHOrZ37hcIlv\yCrbjDoeY1idiCKQ2SxNVYh[2WubIOgZZN{\XO|ZXSgZZMh\G:5boLl[5Vt[XSrb36gc4Yh[3m2b4PvcIlkKEG6aX6yJJBzd3SnaX6g[ZhxemW|c3nvckBifCB3IIXNJIFnfGW{IEG4JIhzeyCkeTDX[ZN1\XKwIHLsc5QhdWW2aH;k MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDJ|M{[4NEc,Ojh{M{O2PFA9N2F-
HCT116 MYXGeY5kfGmxbjDhd5NigQ>? MojSOUB2VQ>? MWCxPEBpenN? M17QVGlvcGmkaYTpc44hd2ZiV370M4JmfGFvY3H0[Y5qdiC|aXfuZYxqdmdiaX6gbJVu[W5iSFPUNVE3KGOnbHzzJIF{e2W|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKGNvTYnjJJBzd3SnaX6g[ZhxemW|c3nvckBifCB3IIXNJIFnfGW{IEG4JIhzeyCkeTDX[ZN1\XKwIHLsc5QhdWW2aH;k M2fhPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkOzOlgxLz5{OEKzN|Y5ODxxYU6=
HCT116 MWfGeY5kfGmxbjDhd5NigQ>? M2HzdlUhfU1? NU\iSGNnOThiaILz M3HSZ2lvcGmkaYTpc44hd2ZiV370M4JmfGFvY3H0[Y5qdiC|aXfuZYxqdmdiaX6gbJVu[W5iSFPUNVE3KGOnbHzzJIF{e2W|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKEO7Y3zpckBFOSCycn;0[YlvKGW6cILld5Nqd25iYYSgOUB2VSCjZoTldkAyQCCqcoOgZpkhX2W|dHXyckBjdG:2IH3leIhw\A>? M162c|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkOzOlgxLz5{OEKzN|Y5ODxxYU6=
HCT116 M1;mZWZ2dmO2aX;uJIF{e2G7 NIDF[XY2KHWP NF60blAyQCCqcoO= MYPJcohq[mm2aX;uJI9nKFewdD;i[ZRiNWOjdHXubY4he2mpbnHsbY5oKGmwIHj1cYFvKEiFVEGxOkBk\WyuczDhd5Nme3OnZDDhd{Bld3ewcnXneYxifGmxbjDv[kB{fXK4aY\pckBxem:2ZXnuJIV5eHKnc4Ppc44h[XRiNTD1UUBi\nSncjCxPEBpenNiYomgW4V{fGW{bjDicI91KG2ndHjv[C=> MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDJ|M{[4NEc,Ojh{M{O2PFA9N2F-
SW480 MnXRSpVv[3Srb36gZZN{[Xl? NUXW[nNvPSC3TR?= MUixPEBpenN? NWPlfYVuUW6qaXLpeIlwdiCxZjDXcpQw[mW2YT3jZZRmdmmwIIPp[45idGmwZzDpckBpfW2jbjDTW|Q5OCClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iCleYTvd49tcWNiYnX0ZU1k[XSnbnnuJJBzd3SnaX6g[ZhxemW|c3nvckBifCB3IIXNJIFnfGW{IEG4JIhzeyCkeTDX[ZN1\XKwIHLsc5QhdWW2aH;k M13kWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkOzOlgxLz5{OEKzN|Y5ODxxYU6=
SW480 MVzGeY5kfGmxbjDhd5NigQ>? NVTkWGlNPSC3TR?= MoHHNVghcHK| NUHuTo9[UW6qaXLpeIlwdiCxZjDXcpQw[mW2YT3jZZRmdmmwIIPp[45idGmwZzDpckBpfW2jbjDTW|Q5OCClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iCleYTvd49tcWNiQYjpclIheHKxdHXpckBmgHC{ZYPzbY9vKGG2IEWgeW0h[W[2ZYKgNVghcHK|IHL5JHdme3Sncn6gZoxwfCCvZYToc4Q> Mly0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{M{O2PFAoRjJ6MkOzOlgxRC:jPh?=
SW480 MmPkSpVv[3Srb36gZZN{[Xl? MkXoOUB2VQ>? MYGxPEBpenN? NI\ofFJKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5ic3nncoFtcW6pIHnuJIh2dWGwIGPXOFgxKGOnbHzzJIF{e2W|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKGNvTYnjJJBzd3SnaX6g[ZhxemW|c3nvckBifCB3IIXNJIFnfGW{IEG4JIhzeyCkeTDX[ZN1\XKwIHLsc5QhdWW2aH;k M3K4NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkOzOlgxLz5{OEKzN|Y5ODxxYU6=
SW480 NUjYbZE2TnWwY4Tpc44h[XO|YYm= MkHqOUB2VQ>? NFzOWosyQCCqcoO= NIf0ZYpKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5ic3nncoFtcW6pIHnuJIh2dWGwIGPXOFgxKGOnbHzzJIF{e2W|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKEO7Y3zpckBFOSCycn;0[YlvKGW6cILld5Nqd25iYYSgOUB2VSCjZoTldkAyQCCqcoOgZpkhX2W|dHXyckBjdG:2IH3leIhw\A>? MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDJ|M{[4NEc,Ojh{M{O2PFA9N2F-
SW480 MYXGeY5kfGmxbjDhd5NigQ>? MVu1JJVO M3e2VVE5KGi{cx?= NIKyRoJKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5ic3nncoFtcW6pIHnuJIh2dWGwIGPXOFgxKGOnbHzzJIF{e2W|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKHO3co\peolvKHC{b4TlbY4h\XiycnXzd4lwdiCjdDC1JJVOKGGodHXyJFE5KGi{czDifUBY\XO2ZYLuJIJtd3RibXX0bI9l M3LERlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkOzOlgxLz5{OEKzN|Y5ODxxYU6=
HCT116 MWHGeY5kfGmxbjDhd5NigQ>? Ml\iNkB2VQ>? MofhN|AhdWmwcx?= NEf6dmVKdmS3Y4Tpc44hd2ZiQV3QT{BxcG:|cHjvdplt[XSrb36gZZQhXGi{LUG3NkBz\XOrZIXlJIlvKGi3bXHuJGhEXDFzNjDj[YxteyCjdDCyJJVOKGGodHXyJFMxKG2rboOgbY4h\2y3Y3;z[UB{fXCybHXt[Y51\WRibXXkbYEh[nliaX3teY5w[myxdDDt[ZRpd2R? NID4NXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEKzN|Y5OCd-MkiyN|M3QDB:L3G+
HCT116 MlX2SpVv[3Srb36gZZN{[Xl? NVPye5RoOiC3TR?= Ml7nN|AhdWmwcx?= MUjJcoR2[3Srb36gc4YhSU2SSzDwbI9{eGixconsZZRqd25iYYSgWIhzNTF5MjDy[ZNq\HWnIHnuJIh2dWGwIFjDWFEyPiClZXzsd{BifCB{IIXNJIFnfGW{IEOwJI1qdnNiaX6gZYJ{\W6lZTDv[kBodHWlb4PlJIJ6KGmvbYXuc4Jtd3RibXX0bI9l M4PTdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkOzOlgxLz5{OEKzN|Y5ODxxYU6=
U2OS NF:0cGhCdnSrdnnyZYwh[XO|YYm= MoG1RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgR4hqc3WwZ4XufYEhfmm{dYOgbY5n\WO2ZXSgbY4hcHWvYX6gWVJQWyClZXzsd{BjgSCUVD3xVGNTKGGwYXz5d4l{NCCHQ{WwQVAvOzcQvF2= NFzhco49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[4PVk4PSd-Mki2PFk6PzV:L3G+
BHK-21 Ml\SRY51cX[rcnHsJIF{e2G7 MnPVRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgR4hqc3WwZ4XufYEhfmm{dYOgNFYyOWGWdzDpcoZm[3SnZDDpckBDUEtvMkGgZ4VtdHNiYomgVnQueVCFUjDhcoFtgXOrczygSWM2OD1yLki1{txO MnXPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4OEm5O|UoRjJ6Nki5PVc2RC:jPh?=
BHK-21 MWPBcpRqfmm{YXygZZN{[Xl? NGrxcGdCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBEcGmtdX7neY56[SC4aYL1d{AxQDFyYmT3JIlv\mWldHXkJIlvKEKKSz2yNUBk\WyuczDifUBTXC2zUFPSJIFv[Wy7c3nzMEBGSzVyPUCuPe69VQ>? NWr3[oE6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2PFk6PzVpPkK4Olg6QTd3PD;hQi=>
BHK-21 Mnu1RY51cX[rcnHsJIF{e2G7 NHrIeWFCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBEcGmtdX7neY56[SC4aYL1d{Bqdm[nY4Tl[EBqdiCESFutNlEh[2WubIOgZpkhWlRvcWDDVkBidmGueYPpd{whTUN3ME2wMlk2|ryP M4XCd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6Nki5PVc2Lz5{OE[4PVk4PTxxYU6=
DAOY M4ftVpFJXFNiYYPzZZk> NWS3NG1yeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFTBU3kh[2WubIO= M3zqeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SJ-GBM2 NE\5N41yUFSVIHHzd4F6 M3KxR5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSj3HRm0zKGOnbHzz M17wSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
A673 NU\N[3RmeUiWUzDhd5NigQ>? M{m0SpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCDNkezJINmdGy| NIjDXWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-MC MnzzdWhVWyCjc4PhfS=> M2rRU5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMW1EKGOnbHzz MkfCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
BT-37 NFfPToFyUFSVIHHzd4F6 M1nYNpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCEVD2zO{Bk\Wyucx?= MnPiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB-EBc1 MVnxTHRUKGG|c3H5 NYT6PFk5eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF7CMWVD[zFiY3XscJM> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Saos-2 M3vqO5FJXFNiYYPzZZk> MlfUdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOjb4OtNkBk\Wyucx?= MlLQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-SH MWrxTHRUKGG|c3H5 MXvxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3TTEBk\Wyucx?= NFzqc|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
NB1643 NHHoSHNyUFSVIHHzd4F6 MnT6dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6EMU[0N{Bk\Wyucx?= MoHzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
LAN-5 M3izN5FJXFNiYYPzZZk> M{SwcJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCOQV6tOUBk\Wyucx?= M33iflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
BT-12 MlvDdWhVWyCjc4PhfS=> M3XtdJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCEVD2xNkBk\Wyucx?= NWHYNGdORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Rh18 NF;aUpFyUFSVIHHzd4F6 NXPUWFVWeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLoNVgh[2WubIO= NGDEe2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
OHS-50 M1qx[pFJXFNiYYPzZZk> NGroTGpyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiT1jTMVUxKGOnbHzz MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
RD MkfzdWhVWyCjc4PhfS=> MWTxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWkRiY3XscJM> MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
MG 63 (6-TG R) M{PJOJFJXFNiYYPzZZk> Mn\IdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE2JIE[zJEg3NVSJIGKpJINmdGy| NGnHfIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Rh41 NFvESIFyUFSVIHHzd4F6 NIntUmxyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUni0NUBk\Wyucx?= NEDMU2M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
NB1643 NEX3dmRyUFSVIHHzd4F6 MYPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDORlE3PDNiY3XscJM> M4[yUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
A673 NVzUZmxneUiWUzDhd5NigQ>? NHjsVmlyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBCPjd|IHPlcIx{MQ>? MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SK-N-MC MX;xTHRUKGG|c3H5 Mn7WdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgV2suVi2PQzDj[Yxtew>? MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
BT-12 M4exdZFJXFNiYYPzZZk> MVvxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDCWE0yOiClZXzsdy=> NV\KUpJIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
LAN-5 MV;xTHRUKGG|c3H5 M2TBcpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJGxCVi13IHPlcIx{ NIXKdXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
DAOY M1;xZpFJXFNiYYPzZZk> MnvzdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgSGFQYSClZXzsdy=> NUXvN4RNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
NB-EBc1 MlPTdWhVWyCjc4PhfS=> NYLTcZpOeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhVkJvRVLjNUBk\Wyucx?= MnvrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SJ-GBM2 NWDGUnNkeUiWUzDhd5NigQ>? MoPsdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgV2ouT0KPMjDj[Yxtew>? MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
BT-37 MYnxTHRUKGG|c3H5 NYewe5NDeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhSlRvM{egZ4VtdHN? Mn;3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
TC32 MUjxTHRUKGG|c3H5 M4q0cJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHREOzJiY3XscJM> M3GwWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
MG 63 (6-TG R) NHTVVGFyUFSVIHHzd4F6 MlztdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUWchPjNiKE[tWGchWiliY3XscJM> NFrMVGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
fibroblast cells MVTxTHRUKGG|c3H5 M{HwcZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJINwdnS{b3ygTIghf2muZDD0fZBmKG[rYoLvZoxie3RiY3XscJM> NUfzeGVrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
U-2 OS MV3xTHRUKGG|c3H5 MV7xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDVMVIhV1NiY3XscJM> NFG3cpc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Rh41 NVHjbnZreUiWUzDhd5NigQ>? M3ixcpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHJpPDFiY3XscJM> M1jLOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
RD MUjxTHRUKGG|c3H5 NE\zZVFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBTTCClZXzsdy=> NV75[IhlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Rh18 MoPwdWhVWyCjc4PhfS=> M4PNcJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHJpOThiY3XscJM> MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh30 MXjxTHRUKGG|c3H5 NGruNYdyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBTcDNyIHPlcIx{ M3G3WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Saos-2 MnfBdWhVWyCjc4PhfS=> M3m4TZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHNid3NvMjDj[Yxtew>? M2PPXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
OHS-50 NGr4TolyUFSVIHHzd4F6 NVrifXJLeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy| MkLzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-SH MlrOdWhVWyCjc4PhfS=> NFnINFFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBUUy2QLWPIJINmdGy| MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SK-N-SH MXvxTHRUKGG|c3H5 NFnhRoVyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCRcoToc4dwdmGuIEPEJJZq[WKrbHn0fUB{[3KnZX6g[o9zKFONLV6tV2gh[2WubIO= M1nwWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SJ-GBM2 M3njN5FJXFNiYYPzZZk> MlLkdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohV3K2aH;nc45idCB|RDD2bYFjcWyrdImgd4Nz\WWwIH\vdkBUUi2JQl2yJINmdGy| MnfTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Daoy M3zKVZFJXFNiYYPzZZk> MmPldWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohV3K2aH;nc45idCB|RDD2bYFjcWyrdImgd4Nz\WWwIH\vdkBF[W:7IHPlcIx{ NVz2blFSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
TC32 Mn;ydWhVWyCjc4PhfS=> NX\oWmFpeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiT4L0bI9od26jbDCzSEB3cWGkaXzpeJkhe2O{ZXXuJIZweiCWQ{OyJINmdGy| NFLlNWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
MG 63 (6-TG R) NH\VWGlyUFSVIHHzd4F6 M3TKTJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KE:{dHjv[49v[WxiM1Sgeoli[mmuaYT5JJNkemWnbjDmc5IhVUdiNkOgLFYuXEdiUjmgZ4VtdHN? NHzoRYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
RD MkHVdWhVWyCjc4PhfS=> MYLxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBQenSqb3fvcoFtKDOGII\pZYJqdGm2eTDzZ5Jm\W5iZn;yJHJFKGOnbHzz NH;G[HI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SW948 NYXGZlRDSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MYe3NkBpenN? M2fCe2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU2e5OFgh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IHPvcI9zcW2ndILpZ{BOXFNiYYPzZZktKEmFNUC9NE4yOc7:TR?= MnT1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{N{S5N|koRjNyMke0PVM6RC:jPh?=
HT-29 NEW2SGhCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M1T3VVczKGi{cx?= MX\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiWLUK5JINmdGy|IHHmeIVzKDd{IHjyd{BjgSClb3zvdolu\XS{aXOgUXRUKGG|c3H5MEBKSzVyPUCuNVPPxE1? NUL0eXJpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyO|Q6OzlpPkOwNlc1QTN7PD;hQi=>
HCT116 MWrBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M2Pnc|czKGi{cx?= M{\oO2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFPUNVE3KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDjc4xwemmvZYTybYMhVVSVIHHzd4F6NCCLQ{WwQVAvPDIQvF2= Mkj1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{N{S5N|koRjNyMke0PVM6RC:jPh?=
HEK293 NUW3SHRVTnWwY4Tpc44h[XO|YYm= Mn;ZOkBpenN? M3HWXWlvcGmkaYTpc44hd2ZiV370N2Eue3SrbYXsZZRm\CCZboSvZoV1[S2lYYTlcolvKHCjdHj3ZZkhcW5iSFXLNlk{KGOnbHzzJJRz[W6|ZnXjeIVlKHerdHigdFh5XE:SRnzhd4gtKFKnbnnscIEhdHWlaX\ldoF{\SCybHHzcYllKHCUTD3UT{BidmRicFzLU{4yKGGodHXyJFYhcHK|IHL5JGR2[WxidH;w[oxie2hibIXjbYZmemG|ZTDy[ZBwenSncjDhd5NigSxiSVO1NF0xNjR3zszN MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJ5NEmzPUc,OzB{N{S5N|k9N2F-
CRC240 MnriRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MkTpO|IhcHK| M1O1eWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQ2LDNlQxKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDjc4xwemmvZYTybYMhVVSVIHHzd4F6NCCLQ{WwQVAvQDoQvF2= NIH3ZnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEK3OFk{QSd-M{CyO|Q6Ozl:L3G+
SW480 Mn22RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NFO5U3U4OiCqcoO= M1[3emFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU2e0PFAh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IHPvcI9zcW2ndILpZ{BOXFNiYYPzZZktKEmFNUC9NE46QM7:TR?= MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJ5NEmzPUc,OzB{N{S5N|k9N2F-
DLD1 M{HFPWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 Mn3IO|IhcHK| NHLKXldCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFTMSFEh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IHPvcI9zcW2ndILpZ{BOXFNiYYPzZZktKEmFNUC9Nk4{Qc7:TR?= NG\TXow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEK3OFk{QSd-M{CyO|Q6Ozl:L3G+
HCT116 NXvaTIp6TnWwY4Tpc44h[XO|YYm= NXTH[YdVPSC3TR?= M{LHSVE5KGi{cx?= NXLzNFNnUW6qaXLpeIlwdiCxZjDXcpQw[mW2YT3jZZRmdmmwIIPp[45idGmwZzDwZZRpf2G7IHnuJIh2dWGwIFjDWFEyPiClZXzsd{BkgXSxc3;sJIx6e2G2ZTDoZZJjd3KrbnegZoV1[S2lYYTlcolvKG23dHH0bY9vKGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBCgGmwMjDwdo91\WmwIHX4dJJme3Orb36gZZQhPSC3TTDh[pRmeiBzODDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz NWfISYxlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyO|Q6OzlpPkOwNlc1QTN7PD;hQi=>
HCT116 NVrNSGJwTnWwY4Tpc44h[XO|YYm= MX61JJVO Ml3uNVghcHK| NVS5UmFOUW6qaXLpeIlwdiCxZjDXcpQw[mW2YT3jZZRmdmmwIIPp[45idGmwZzDwZZRpf2G7IHnuJIh2dWGwIFjDWFEyPiClZXzsd{BkgXSxc3;sJIx6e2G2ZTDoZZJjd3KrbnegZoV1[S2lYYTlcolvKG23dHH0bY9vKGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBj\XSjLXPheIVvcW5icILveIVqdiCneIDy[ZN{cW:wIHH0JFUhfU1iYX\0[ZIhOThiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= NHnWXFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEK3OFk{QSd-M{CyO|Q6Ozl:L3G+
SW480 NFjJUo1HfW6ldHnvckBie3OjeR?= MXW1JJVO MnnZNVghcHK| NY\HclliUW6qaXLpeIlwdiCxZjDXcpQw[mW2YT3jZZRmdmmwIIPp[45idGmwZzDwZZRpf2G7IHnuJIh2dWGwIGPXOFgxKGOnbHzzJIN6fG:|b3ygcJl{[XSnIHjhdoJwemmwZzDBVGMhdXW2YYTpc44h[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJGF5cW5{IIDyc5RmcW5iZYjwdoV{e2mxbjDheEA2KHWPIHHmeIVzKDF6IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= NYTCZppHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyO|Q6OzlpPkOwNlc1QTN7PD;hQi=>
SW480 NYLkbVNMTnWwY4Tpc44h[XO|YYm= NWDk[3ZEPSC3TR?= MkWzNVghcHK| NFT5OGpKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5ic3nncoFtcW6pIIDheIh4[XliaX6gbJVu[W5iU2e0PFAh[2WubIOgZ5l1d3OxbDDsfZNifGViaHHyZo9zcW6pIFHQR{BufXSjdHnvckBie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZoV1[S2lYYTlcolvKHC{b4TlbY4h\XiycnXzd4lwdiCjdDC1JJVOKGGodHXyJFE5KGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? M3WwUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMke0PVM6Lz5|MEK3OFk{QTxxYU6=
CRC240 NX\X[|hLTnWwY4Tpc44h[XO|YYm= NFnUS5M2KHWP M1fkU|E5KGi{cx?= NGDvUGhKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPheIVvcW5ic3nncoFtcW6pIIDheIh4[XliaX6gbJVu[W5iQ2LDNlQxKGOnbHzzJIN6fG:|b3ygcJl{[XSnIHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCDeHnuNkBxem:2ZXnuJIV5eHKnc4Ppc44h[XRiNTD1UUBi\nSncjCxPEBpenNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ Mn;lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{N{S5N|koRjNyMke0PVM6RC:jPh?=
CRC240 M3y4UmZ2dmO2aX;uJIF{e2G7 NIrEc4g2KHWP NHj5PVgyQCCqcoO= M{fiW2lvcGmkaYTpc44hd2ZiV370M4JmfGFvY3H0[Y5qdiC|aXfuZYxqdmdicHH0bJdigSCrbjDoeY1idiCFUlOyOFAh[2WubIOgZ5l1d3OxbDDsfZNifGViYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGKndHGtZ4F1\W6rbjDwdo91\WmwIHX4dJJme3Orb36gZZQhPSC3TTDh[pRmeiBzODDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz NXO4PHlRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyO|Q6OzlpPkOwNlc1QTN7PD;hQi=>
CCD-841-CoN Ml;BSpVv[3Srb36gZZN{[Xl? NHvTXmMyKHWP NIXMOJVUfGmvdXzheIlwdiCxZjDtbZRw[2ixbnTybYFtKHKnc4DpdoF1cW:wIHnuJIh2dWGwIFPDSE05PDFvQ3;OJINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKG:6eXflckBkd26|dX3weIlwdiC{YYTlJIF1KDFidV2gbY4heHKnc3XuZ4Uhd2ZiRlPDVEBjgSCyb3zhdo9oemGyaHnjJIFv[Wy7c3nz NFPOeJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEK3OFk{QSd-M{CyO|Q6Ozl:L3G+
CCD-841-CoN MlLISpVv[3Srb36gZZN{[Xl? M3PDRlAvOSC2bzCxNEB2VQ>? NHu3OGszKGi{cx?= M1HBXmlv\HWldHnvckBw\iCDTWDLJJBpd3OyaH;yfYxifGmxbjDpckBpfW2jbjDDR2QuQDRzLVPvUkBk\WyuczDheEAxNjFidH:gNVAhfU1iYX\0[ZIhOiCqcoOgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| MkXDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{N{S5N|koRjNyMke0PVM6RC:jPh?=
CRC240 NUCwWplYSW62aYT1cY9zKGG|c3H5 MVu3NkBu\y:tZx?= MVTBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCFUlOyOFAh[2WubIOgfIVvd2e{YX\0[YQhcW5iTl;EM3NEUURibX;1d4Uh[XRiN{KgcYcwc2duIIDvJIFldWmwaYP0[ZJm\CBzMTDkZZl{KGGwZDDt[YF{fXKnZDDvckBl[XliNDygPEBlfXKrbnegZ49ueG:3bnSg[I9{cW6pIHHu[EBl[XliMUGgdI9{fCCuYYP0JIRwe2V? MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJ5NEmzPUc,OzB{N{S5N|k9N2F-
U2OS MlLySpVv[3Srb36gZZN{[Xl? MoTqOkBpenN? MlfPTY5lfWO2aX;uJI9nKEeIUD30ZYdo\WRiRorkNUApfW6tbn;3ckBwemmpaX6pJIlvfGW{bnHsbZpifGmxbjDlfJBz\XO|ZXSgbY4hcHWvYX6gWVJQWyClZXzsd{BifCBzMj61JIFnfGW{IE[gbJJ{KGK7IHPvcoZw[2GuIH3pZ5Jwe2OxcHnjJI1mfGixZB?= M2LYUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMke0PVM6Lz5|MEK3OFk{QTxxYU6=
A549 M1juO2Z2dmO2aX;uJIF{e2G7 MXW2JIhzew>? MU\JcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKEF3NEmgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4h[2G|cHHz[UA{NzdiYXP0bZZqfHliYX\0[ZIhPiCqcoOgZpkh[2G|cHHz[UBodG9iMz:3JIF{e2G7 NH2x[lM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEO3NVA3PCd-M{CzO|ExPjR:L3G+
Caco2 Mo[zSpVv[3Srb36gZZN{[Xl? MnLKOkBpenN? NYDX[HhQUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDDZYNwOiClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDjZZNx[XOnIEOvO{Bi[3Srdnn0fUBi\nSncjC2JIhzeyCkeTDjZZNx[XOnIHfsc{A{NzdiYYPzZZk> NFrrZlg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEO3NVA3PCd-M{CzO|ExPjR:L3G+
AsPC1 M{L1[2Z2dmO2aX;uJIF{e2G7 NXnMU4ZKPiCqcoO= M4roU2lv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iQYPQR|Eh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iY3HzdIF{\SB|L{egZYN1cX[rdImgZYZ1\XJiNjDodpMh[nliY3HzdIF{\SCpbH:gN{84KGG|c3H5 MmjVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB|N{GwOlQoRjNyM{exNFY1RC:jPh?=
HEK293 NXrFOHdYTnWwY4Tpc44h[XO|YYm= NWT5[WNrQCCqcoO= M2SwfWlvcGmkaYTpc44hd2ZiV370N2Eue3SrbYXsZZRm\CCZboSvZoV1[S2lYYTlcolvKHCjdHj3ZZkhcW5iSFXLNlk{KGOnbHzzJJRz[W6|ZnXjeIVlKHerdHigdFh5XE:SRnzhd4gtKFKnbnnscIEhdHWlaX\ldoF{\SCybHHzcYllKHCUTD3UT{BidmRicFzLU{4yKGGodHXyJFghcHK|IHL5JGR2[WxidH;w[oxie2hibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyPUCuN|TPxE1? M124VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyNUWxPVAyLz5|MEW1NVkxOTxxYU6=
LN229 NWfOZVk{SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MofJOUBl[Xm| NYrj[otKSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDMUlIzQSClZXzsd{Bi\nSncjC1JIRigXNiYomgRYxidWG{Qnz1[UBie3OjeTygTWM2OD1yLkROwG0> NIHP[3A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEW4N|I1QCd-M{C1PFMzPDh:L3G+
T98G NWnFNXpYSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MXu1JIRigXN? NXTGOWY{SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDUPVhIKGOnbHzzJIFnfGW{IEWg[IF6eyCkeTDBcIFu[XKEbIXlJIF{e2G7LDDJR|UxRTBwM988US=> MkPuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB3OEOyOFgoRjNyNUizNlQ5RC:jPh?=
U87 MmXCRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NYDrVGlTPSCmYYnz NIHoNHhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGW4O{Bk\WyuczDh[pRmeiB3IHThfZMh[nliQXzhcYFzSmy3ZTDhd5NigSxiSVO1NF0xNjQQvF2= M3u3Z|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyNUizNlQ5Lz5|MEW4N|I1QDxxYU6=
U138MG M1roVWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MV21JIRigXN? NHK3PYNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGWxN|hOTyClZXzsd{Bi\nSncjC1JIRigXNiYomgRYxidWG{Qnz1[UBie3OjeTygTWM2OD1yLkROwG0> M2rPc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyNUizNlQ5Lz5|MEW4N|I1QDxxYU6=
U373 M2r1eGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MnzWOUBl[Xm| MVHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFV|N{OgZ4VtdHNiYX\0[ZIhPSCmYYnzJIJ6KEGuYX3hdmJtfWViYYPzZZktKEmFNUC9NE4{|ryP MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODV6M{K0PEc,OzB3OEOyOFg9N2F-
HL60 MoDLR5l1d3SxeHnjbZR6KGG|c3H5 NIDVcFE{KGSjeYO= NIrSbnJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJVDZyIHPlcIx{KGmwY4XiZZRm\CCob4KgN{Bl[Xm|IHL5JGNmdGyWaYTldk1IdG9ibIXtbY5me2OnboSgZZN{[XluIFnDOVA:OC5|zszN MoPOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF{NUO1NlkoRjNzMkWzOVI6RC:jPh?=
KG1 MXHDfZRwfG:6aXPpeJkh[XO|YYm= M{LaWFMh\GG7cx?= Mn3KR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2cyKGOnbHzzJIlv[3WkYYTl[EBnd3JiMzDkZZl{KGK7IFPlcIxVcXSncj3HcI8hdHWvaX7ld4NmdnRiYYPzZZktKEmFNUC9NE4{Ps7:TR?= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTJ3M{WyPUc,OzF{NUO1Nlk9N2F-
Jurkat M3mwPWN6fG:2b4jpZ4l1gSCjc4PhfS=> M13jVlMh\GG7cx?= NH7QT3lEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBLfXKtYYSgZ4VtdHNiaX7jeYJifGWmIH\vdkA{KGSjeYOgZpkhS2WubGTpeIVzNUeubzDseY1qdmW|Y3XueEBie3OjeTygTWM2OD1yLkVOwG0> MlP5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF{NUO1NlkoRjNzMkWzOVI6RC:jPh?=
NALM6 MW\DfZRwfG:6aXPpeJkh[XO|YYm= MorxN{Bl[Xm| NH\nbppEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBPSUyPNjDj[YxteyCrbnP1ZoF1\WRiZn;yJFMh\GG7czDifUBE\WyuVHn0[ZIuT2yxIHz1cYlv\XOlZX70JIF{e2G7LDDJR|UxRTBwNkNOwG0> NWmzU41sRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{GyOVM2OjlpPkOxNlU{PTJ7PD;hQi=>
HEK293 NYK2VHN5TnWwY4Tpc44h[XO|YYm= M3rQWVkxKG2rboO= NHTofo5KdmirYnn0bY9vKG:oIFvJXE1TVHWlTjDmeZNm\CCFQmCgLJVvc26xd36gc5Jq\2mwKTDibY5lcW6pIITvJGtKTC2UTIXjR{BnfXOnZDDDVmVDKCi3bnvuc5dvKG:{aXfpckkhfHKjboPm[YN1\WRiaX6gbJVu[W5iSFXLNlk{KGOnbHzzJJBz\WmwY4XiZZRm\CC5aYToJINwdXCxdX7kJFMxKG2rboOgZoVnd3KnIHLl[o9z\SCob4Lzb49tcW5iYXTkbZRqd25iYX7kJI1m[XO3cnXkJIFnfGW{IEmwJI1qdnNiaX6gdJJme2WwY3Wgc4Yh[2:nbHXueIVz[XpuIFnDOVA:OS53M988US=> NWjFb3V1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{GyOVM2OjlpPkOxNlU{PTJ7PD;hQi=>
293beta5 NVfR[WFuSW62aY\pdoFtKGG|c3H5 NX\lUFFEPyCmYYnz NVq4V5V4SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUHWvYX6gZYRmdm:4aYL1d{A2KGmwZnXjeIVlKGmwIEK5N4JmfGF3IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YheGyjcYXlJIZwem2jdHnvckBqdmO3YnH0[YQh\m:{IEeg[IF6eyCkeTDHSnAhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NF0xNjcQvF2= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OjB2NUKzPUc,OzJyNEWyN|k9N2F-
A549 MYPBcpRqfmm{YXygZZN{[Xl? MoDSOFghcHK| M{LSNWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGh2dWGwIHHk[Y5wfmm{dYOgOUBqdm[nY4Tl[EBqdiCqdX3hckBCPTR7IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yhfmm{YXyg[Y51enliYX\0[ZIhPDhiaILzJIJ6KEeIUDDy[ZBwenSncjDn[Y5mKGKjc3XkJIF{e2G7LDDJR|UxRTFwMkNOwG0> MoLxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzJyNEWyN|koRjN{MES1NlM6RC:jPh?=
A549 MV7DfZRwfG:6aXPpeJkh[XO|YYm= M{nhPFQ5KGi{cx?= MVHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZktKEOFNUC9NlIvQc7:TR?= NIf2W2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MkC0OVI{QSd-M{KwOFUzOzl:L3G+
Vero NVTD[3Q3TnWwY4Tpc44h[XO|YYm= MnL0WoVzdyClZXzsd{B3cWGkaXzpeJkheUiWUzDmc5IhYmmtYTD2bZJ2eyCrbnjpZol1d3K| NITyVXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|M{KyPVU1PSd-M{OyNlk2PDV:L3G+
Vero MmDJRY51cX[rcnHsJIF{e2G7 NV:1UlVmOjRiaIK= MUXBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDTRXJUNUOxVj2yJEh3cXKjbDD0bZRmeilibXXhd5Vz\WRiYomgdIxieXWnIHHzd4F6KGmwIG\ldo8h[2WubIOgZZQhVU:LIECuNFEzPSCjZoTldkAzPCCqcjygTWM2OD1yLkK4{txO MmG3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ1QC9pPlPoSW1DVDxxYU6=
skeletal myoblast cells M3vESWN6fG:2b4jpZ4l1gSCjc4PhfS=> NYHzSIZOPzJiaB?= MmXLSG5FUTpiQ4n0c5RwgGmlaYT5JIlvKF[rdILvMEA4OiCqb4XyMEBqdiC{YYSgd4tmdGW2YXygcZlw[myjc4SgZ4VtdHNuIFnDOVA:Oi5|zszN MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxOFQ5Nyd-Q3jFUWJNRC:jPh?=
VERO-E6 NGq1cWdHfW6ldHnvckBie3OjeR?= M1e0R|Q5KGi{cx?= NX7PdVJ7TGW2ZYLtbY5ifGmxbjDv[kBKSzVyII\hcJVmeyCob4KgbY5pcWKrdHnvckBw\iCVQWLTMWNwXi1{IHnu[JVk\WRiY4n0c5RwgGmlaYT5JI9nKF[HUl:tSVYh[2WubIOgZYZ1\XJiNEigbI92enNiIHX4dI9{fXKnIITvJFAvODFiTV;JJHNCWlNiQ3;WMVIhfmm{dYOgZpkhcGmpaDDjc451\W62IHntZYdqdmduIFnDOVA:PC5zzszN M4PRVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PDhxJ{7DbGVOSkx:L3G+
Assay
Methods Test Index PMID
Western blot p-STAT3 / STAT3 / c-Myc / Survivin ; p-BCR-ABL / BCR-ABL ; p-STAT5 / STAT5 / p-AKT / AKT / p-ERK / ERK ; β-catenin 25970160 29358661 27652012
Growth inhibition assay Cell viability 28418862
In vivo Niclosamide(40 mg/kg/d, i.p.) inhibits growth of xenografted AML cells in nude mice bearing HL-60 xenograft tumors. [4]

Protocol (from reference)

Kinase Assay:

[1]

  • Protein Kinase profiling assay:

    Assay for 22 different proteins kinases is carried out by ProQinase Gmbh. All of the protein kinases are expressed either in Sf9 insect cells or in E.coli as recombinant GST-fusion proteins or His-tagged proteins. Protein kinases are purified by affinity chromatography using either GSH-agarose or Ni_NTH-agarose. A radiometric protein kinase assay is used for measuring the kinase activity of the 22 protein kinases. Briefly, for each protein kinase, 50 μL reaction cocktail containing 60 mM HEPES-NaOH, 3 mM MgCl2, 3 mM MnCl2, 3 μM Na-orthovanadate, 1.2 mM DTT, 50 μg/mL PEG20000, 1 μM [γ-33P]-ATP, Niclosamide, adequate amount of enzyme and its substrate. The PKC-alpha assay additionally contain 1 mM Cacl2, 4 mM EDTA, 5 μg/mL phosphatidylserine and 1 μg/mL 1, 2-Dioleyl-glycerol. The reaction cocktails are incubated at 37 °C for 60 minutes and stop with 50 μL 2% (v/v) H3PO4. Incorporation of 33Pi is determined with a microplate scintillation counter. The activities and the IC50 values are calculated using Quattro Workflow V2.28.

Cell Research:

[1]

  • Cell lines: Hela, A549, Du145, HT-29, A431, PC3 cells
  • Concentrations: 16 μM
  • Incubation Time: 72 hours
  • Method:

    Cells are plated in 96-well culture plates with cell density of 3-4 × 103 cells/well and treat with Niclosamide by adding 100 μL medium containing Niclosamide of various concentrations on the second day. After 72-hour's treatment, MTT is added to each well and incubated for additional 4-5 hours, and the absorbance is measured on a microplate reader at 570nm. Cell growth inhibition is evaluated as the ratio of the absorbance of the sample to that of the control. The results are representative of at least 3 independent experiments.

Animal Research:

[4]

  • Animal Models: nude mice bearing HL-60 xenograft tumors.
  • Dosages: 40 mg/kg
  • Administration: Intraperitoneal injection

Solubility (25°C)

In vitro

DMF 12 mg/mL warmed
(36.68 mM)
DMSO Insoluble
Water Insoluble

Chemical Information

Molecular Weight 327.12
Formula

C13H8Cl2N2O4

CAS No. 50-65-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC(=C(C=C1[N+](=O)[O-])Cl)NC(=O)C2=C(C=CC(=C2)Cl)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05188170 Not yet recruiting Drug: Niclosamide Acute Myeloid Leukemia (AML) Stanford University July 2022 Phase 1
NCT05168644 Recruiting Drug: Niclosamide Inhalation Powder|Drug: Placebo Healthy TFF Pharmaceuticals Inc. November 14 2021 Phase 1
NCT04436458 Not yet recruiting Drug: Niclosamide Oral Tablet|Drug: Placebo COVID First Wave Bio Inc. June 2021 Phase 2
NCT04644705 Completed Drug: Niclosamide|Drug: Placebo Healthy Volunteers Charité Research Organisation GmbH|Bayer November 2 2020 Phase 1
NCT04592835 Not yet recruiting Drug: DWRX2003 COVID-19 Patients Daewoong Pharmaceutical Co. LTD.|Novotech (Australia) Pty Limited October 19 2020 Phase 1
NCT04524052 Not yet recruiting Drug: DWRX2003|Drug: Placebo Healthy Daewoong Pharmaceutical Co. LTD. August 2020 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Niclosamide (BAY2353) | Niclosamide (BAY2353) supplier | purchase Niclosamide (BAY2353) | Niclosamide (BAY2353) cost | Niclosamide (BAY2353) manufacturer | order Niclosamide (BAY2353) | Niclosamide (BAY2353) distributor