For research use only.

Catalog No.S7337

28 publications

SH-4-54 Chemical Structure

Molecular Weight(MW): 610.59

SH-4-54 is a potent STAT inhibitor with KD of 300 nM and 464 nM for STAT3 and STAT5, respectively.

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Selleck's SH-4-54 has been cited by 28 publications

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Biological Activity

Description SH-4-54 is a potent STAT inhibitor with KD of 300 nM and 464 nM for STAT3 and STAT5, respectively.
STAT3 [1]
(Cell-free assay)
STAT5 [1]
(Cell-free assay)
300 nM(Kd) 464 nM(Kd)
In vitro

SH-4-54 shows unprecedented cytotoxicity in human glioblastoma brain cancer stem cells (BTSCs), while has no toxicity in human fetal astrocytes. In addition, SH-4-54 effectively suppresses STAT3 phosphorylation and its downstream transcriptional targets. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human 127EF cells NXrjSWhQS3m2b4TvfIlkyqCjc4PhfS=> NGDv[Yw{KGSjeYO= MlOyR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gNVI4TUZiY3XscJMh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgN{Bl[Xm|IHL5JGFt[W2jcjDCcJVmKGG|c3H5MEBKSzVyPU[2JI5O NXq1cJVwOjR7MEC2NVI>
human 30M cells M1Pod2N6fG:2b4jpZ:Kh[XO|YYm= M1XjeFMh\GG7cx?= MX;DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjCzNG0h[2WubIOgZZN{\XO|ZXSgZZMh[2WubDD2bYFjcWyrdImgZYZ1\XJiMzDkZZl{KGK7IFHsZY1ieiCEbIXlJIF{e2G7LDDFR|UxRTBwMTFOwG0> NGq0ZYMzPDlyME[xNi=>
human 84EF cells M{PRS2N6fG:2b4jpZ:Kh[XO|YYm= NWK3TpduOyCmYYnz NHL0R|JEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckA5PEWIIHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFMh\GG7czDifUBCdGGvYYKgRox2\SCjc4PhfUwhUUN3ME2wMlExOiEQvF2= MWKyOFkxODZzMh?=
human 67EF cells MoPJR5l1d3SxeHnjxsBie3OjeR?= M3ftdFMh\GG7cx?= MlvBR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gOldGTiClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBi\nSncjCzJIRigXNiYomgRYxidWG{IFLseYUh[XO|YYmsJGlEPTB;MD6xNFYh|ryP NY\WTmtbOjR7MEC2NVI>
human 73EF cells NFTpSpZEgXSxdH;4bYPDqGG|c3H5 MVSzJIRigXN? MofhR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gO|NGTiClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBi\nSncjCzJIRigXNiYomgRYxidWG{IFLseYUh[XO|YYmsJGlEPTB;MD6xOlIh|ryP NUS1TVNsOjR7MEC2NVI>
human 25EF cells MWHDfZRwfG:6aXRCpIF{e2G7 MY[zJIRigXN? M4nabGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJFI2TUZiY3XscJMh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgN{Bl[Xm|IHL5JGFt[W2jcjDCcJVmKGG|c3H5MEBKSzVyPUCuNlM1KM7:TR?= NEHoWXIzPDlyME[xNi=>
human 73M cells Mnf2R5l1d3SxeHnjxsBie3OjeR?= M1G0eFMh\GG7cx?= NIDUSoxEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckA4O01iY3XscJMh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgPEBl[Xm|IHL5JGFt[W2jcjDCcJVmKGG|c3H5MEBKSzVyPUGuNFMh|ryP MViyOFkxODZzMh?=
human 147EF cells M1zh[mZ2dmO2aX;uJIF{e2G7 NYPBWGtLOi15MjDo NVH2RnVOUW6qaXLpeIlwdiCxZjDTWGFVOyCrbjDoeY1idiBzNEfFSkBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hd2ZicHjvd5Bpd3K7bHH0[YQh[3mlbHnuJGQyKGyndnXsJIFnfGW{IEKgeI8hPzJiaILzJIJ6KFenc4Tldo4h[myxdITpcoch[W6jbInzbZM> M4LYe|I1QTByNkGy

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
LMP1 / p-STAT3 / STAT3 ; 

PubMed: 28445949     

Farage cells were treated with 0.1, 1, or 5 μM SH-4-54 for 3 h. The cells were lysed followed by western blotting with anti-LMP1, pSTAT3, and pNF-κB p65 (Ser536) antibodies.

In vivo In mice orthotopically xenografted with BT73, SH-4-54 (10 mg/kg i.p.) exhibits BBB permeability, potently suppresses glioma tumor growth, and inhibits pSTAT3. [1]


Kinase Assay:


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Surface Plasmon Resonance (SPR) studies:

The binding experiments are carried out on a ProteOn XPR36 biosensor at 25°C using the HTE sensor chip. The flow cells of the sensor chip are loaded with a nickel solution at 30 μL/min for 120 s to saturate the Tris–NTA surface with Ni(II) ions. Purified His-tagged STAT3 and STAT5 in PBST buffer (PBS with 0.005% (v/v) Tween-20 and 0.001% DMSO pH 7.4) is injected in the first and second channels of the chip respectively in the vertical direction at a flow rate of 25 μg/μL for 300 s, which attained, on average, ~8000 resonance unit (RU). After a wash with PBST buffer, inhibitors binding to the immobilized proteins is monitored by injecting a range of concentrations along with a blank at a flow rate of 100 μL/min for 200 s for each of these small molecules. When the injection of the small molecule inhibitor is completed, running buffer is allowed to flow over the immobilized substrates for the non-specifically bound inhibitors to dissociate for 600 s. Following dissociation of the inhibitors, the chip surface is regenerated with an injection of 1 M NaCl at a flow rate of 100 μL/ml for 18 s. Interspot channel reference is used for non-specific binding corrections and the blank channel used with each analyte injection served as a double reference to correct for possible baseline drift. Data are analyzed using ProteOn Manager Software version 3.1. The Langmuir 1:1 binding model was used to determine the KD values.
Cell Research:


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  • Cell lines: BTSC lines 25M, 67EF, 73EF, 84EF and 127EF
  • Concentrations: ~25 μM
  • Incubation Time: 72 hours
  • Method:

    BTSC spheres are dissociated to single cells with the enzyme Accumax, seeded at 1500 cells/ 96-well and treated with drug or vehicle (DMSO) one day after plating. Cytotoxicity studies are repeated independently using BTSC lines 25M, 67EF, 73EF, 84EF and 127EF. BTSC spheres are dissociated to single cells as above and plated in 96 well plates in triplicate at 3000 cells/ 96-well. In both sets of experiments drugs are used as serial dilutions within the range of 5 μM to 100 nM in the first set and 25 μM to 10 nM. Cell viability following drug treatment is assessed three days later using the alamarBlue assay according to the manufacturer’s instructions. All culture experiments are performed in triplicate with a minimum of three wells per condition.

    (Only for Reference)
Animal Research:


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  • Animal Models: NOD-SCID bearing ed with BT73 glioma xenografts
  • Dosages: Suspended in 50% polyethylene glycol 300 in water
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (163.77 mM)
Ethanol 50 mg/mL warmed (81.88 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 610.59


CAS No. 1456632-40-8
Storage powder
in solvent
Synonyms N/A
Smiles CN(CC(=O)N(CC1=CC=C(C=C1)C2CCCCC2)C3=CC=C(C=C3)C(O)=O)[S](=O)(=O)C4=C(F)C(=C(F)C(=C4F)F)F

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)
% DMSO % % Tween 80 % ddH2O

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID