Stattic

Catalog No.S7024

Stattic Chemical Structure

Molecular Weight(MW): 211.19

Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1.

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In DMSO USD 90 In stock
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Cited by 54 Publications

Purity & Quality Control

Choose Selective STAT Inhibitors

Biological Activity

Description Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1.
Features Stattic is the first non-peptide small molecule with inhibitory activity against STAT3 SH2 domain regardless of the STAT3 phosphorylation state in vitro.
Targets
STAT3 [1]
(Cell-free assay)
5.1 μM
In vitro

Stattic inhibits binding of a phosphotyrosine-containing peptide derived from the gp130 receptor to the STAT3 SH2 domain in a strongly temperature-dependent manner. Stattic only has a very weak effect on binding of a tyrosinephosphorylated peptide to the SH2-domain of the tyrosine kinase Lck. And it doesn’t inhibit dimerization of two other dimeric transcription factors (c-Myc/Max and Jun/Jun). It also inhibits fluorescein-labeled phosphopeptides to the SH2 domains of STAT1 and STAT5b. Stattic selectively inhibits DNA binding of STAT3 homodimers at a concentration of 10 μM. Shown to inhibit cellular phosphorylation of STAT3 at Tyr705 with little effect towards STAT1 phosphorylation at Tyr701 (in HepG2 cells) or the phosphorylation of JAK1, JAK2, and c-Src (in MDA-MB-231 and MDA-MB-235S cells). Stattic increases the apoptotic rate of STAT3-dependent breast cancer cell lines. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H9c2 NHL6VJdHfW6ldHnvckBCe3OjeR?= NVzodFRXOTEkgJpOwG0> MlHsOQKBkWh? NXv4Om5ZemW4ZYLz[ZMhfGinIHXm[oVkfHNib3[gTWwuOjd? MVWyOlM{QTZ|Mx?=
NPC  NHnDNIpHfW6ldHnvckBCe3OjeR?= NILyWpMxNTdwNTFCuW0> NVn4Slgy[WKxbHnzbIV{KEWPVD3sbYtmKG2xbHXjeYxieiCjbITldoF1cW:wczygZY5lKGOnbHygcYloemG2aX;uJIFv\CCrbo\hd4lwdiCrbnT1Z4VlKGK7IGLLTXAhc26xY3vkc5dv M1O1c|I2QTF3NEOw
HASMC NU\TOXFGTnWwY4Tpc44hSXO|YYm= NHfnbXkyNjJ3LUWg{txO NGXrdFIzOCCvaX6= MkLNSG1UV8Li M17EdolvcGmkaYTzJJAuMFlrLWPURXQuOSx|LEWgd4lodmGuc9Mg NWH3eox6OjV6NEm2NlI>
H9c2  NUL4XZpvTnWwY4Tpc44hSXO|YYm= M4\iW|IwOTBizszN NF\VbnYzKGh? M3\GWGROW00EoB?= MnTNZYJzd2ejdHXzxsB1cGViY4n0c5Bzd3SnY4TpeoUh\W[oZXP0d{Bw\iCLTD2yO{Bi\2GrboP0JHNJ MWmyOVgzODlyNx?=
A431 MoP6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH36VFgzyqEQvF2= MVWyxsBp MkjQZoxw[2u|IFXHSk1z\X[ncoPl[EBl\WO{ZXHz[ZMhcW5iY3XscEB3cWGkaXzpeJk> MWmyOVczODR|NR?=
A431 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVrXcIs1OsLizszN MYiyxsBp M2TwXIlv[3KnYYPld{BqdiCjcH;weI9{cXNiaX7keYNm\CCkeTDzbIlsd26rbh?= MXuyOVczODR|NR?=
SiHa MXzD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NF;jZmI2NTd3IH7N NWexUVFLOjUEoHlCpC=> MVPzbI94eyCvb4LwbI9td2e7IH;mJIEhfHmyaXPhcEBieG:ydH;0bYMh[2WubDDhcoQh\G:|ZT3k[ZBmdmSnboSgcI9{eyCxZjDj[YxtKH[rYXLpcIl1gcLi M3rxPVI2PTN7NkS0
SiHa NGDpNVRHfW6ldHnvckBCe3OjeR?= M{Dy[VUuPzVibl2= M3PrflI1yqCqwrC= NFnqeG1z\WS3Y3XzJJRp\cLicHjvd5Bpd3K7bHH0bY9vKGG2IITo[UB1gXKxc3nu[UBz\XOrZIXlJFcxPcLi NYT6eJNbOjV3M{m2OFQ>
ECA109 M4K4OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVPaSIhlOC1{MDFOwG0> M3\oVlI1yqCqwrC= NVvzPI53UUN3ME21MlUxKM7:TR?= M4C1cVI2PDl{NEiw
TE13 M{\zN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NG\TeGExNTJyIN88US=> NHjF[4MzPMLiaNMg MlTWTWM2OD14LkG1xsDPxE1? NFfmeHozPTR7MkS4NC=>
KYSE150  NEnYOVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mof1NE0zOCEQvF2= NYnVXFluOjUEoHlCpC=> NU\ldpZLUUN3ME2xNk43PMLizszN MnvENlU1QTJ2OEC=
ECA109 MoTsR4xwdm:pZX7pZ{BUfXK4aY\hcEBCe3OjeR?= M{nPTVAvPcLizszN MoPzNlTDqGkEoB?= M13qbJN2eHC{ZYPz[ZMhfGinIHPsc45w\2WwaXOg[o9zdWG2aX;u M1LNZlI2PDl{NEiw
TE13 NILlWWVEdG:wb3flcolkKFO3co\peoFtKEG|c3H5 MUSwMlXDqM7:TR?= NE\BVIszPMLiaNMg MVHzeZBxemW|c3XzJJRp\SClbH;uc4dmdmmlIH\vdo1ifGmxbh?= NHzWfJQzPTR7MkS4NC=>
KYSE150  M{flWGNtd26xZ3XubYMhW3W{dnn2ZYwhSXO|YYm= NX60fIdEOC53wrFOwG0> MWOyOOKhcMLi MWLzeZBxemW|c3XzJJRp\SClbH;uc4dmdmmlIH\vdo1ifGmxbh?= MmfJNlU1QTJ2OEC=
ECA109 NXTWTJFOTnWwY4Tpc44hSXO|YYm= MXqwMlXDqM7:TR?= MoHYNlTDqGkEoB?= M2PVVoVvcGGwY3XzJGlTNWmwZIXj[YQh\2WwZYLheIlwdiCxZjDEV2J{ NGGwXWwzPTR7MkS4NC=>
PC3M-1E8 MYHGeY5kfGmxbjDBd5NigQ>? MVqyMlUwPS9zMDFOwG0> Ml7INE01KGh? M2DJRYlvcGmkaYTzJJRp\SCVVFHUN{Bi[3SrdnH0bY9vKGmwIHGg[I9{\S1iYX7kJJRqdWVvZHXw[Y5l\W62IH3hco5mesLi NGjjdZkzPTJ4MUO2OS=>
PC3M-1E8 MYDGeY5kfGmxbjDBd5NigQ>? MX:xNEDPxE1? Mm\QNlQhcA>? MmDD[I94dnKnZ4XsZZRmeyCEY3ytfGwtKHO3co\peolvKGGwZDDjMW16[w>? M3ewdlI2OjZzM{[1
PC3M-1E8 Mle2SpVv[3Srb36gRZN{[Xl? MUKxNEDPxE1? MYWyOEBp MlHBbY5pcWKrdIOgTWwuPiCrbnT1Z4VlKFOWQWSzJIFkfGm4YYTpc44h[W6mIITo[UBKVC14LXnu[JVk\WRiU2TBWFMh[WO2aY\heIlwdg>? M2CyUVI2OjZzM{[1
PC3M-1E8 MmjjR4xwdm:pZX7pZ{BUfXK4aY\hcEBCe3OjeR?= Ml\MNk42NzVxMUCg{txO MXHpcohq[mm2c9MgeIhmKGOxbH;ufUBnd3KvYYTpc44he2mpbnnmbYNidnSueR?= MXiyOVI3OTN4NR?=
MDA-MB-231 M1\VUGZ2dmO2aX;uJGF{e2G7 Mn;GNlAh|ryP M3;EWFIhcA>? MYflfIhq[mm2czDTcoFqdCCjbnSgSU1k[WSqZYLpckBmgHC{ZYPzbY9v NXn2NGJoOjVzNUOzOFk>
H9c2 MUHGeY5kfGmxbjDBd5NigQ>? NGPifHYzOCEEtV2= NWDweWNWOzBibXnu MYPEUXNQyqB? NVnZSnZi[WKxbHnzbIV{KHC{b4Dv[o9tNWmwZIXj[YQhSUuWIIDoc5NxcG:{eXzheIlwdiCjdDDic5RpKHOnckS3N{BidmRidHjyN|A5 Mn6wNlUyODVyNke=
HaCaT  Mnq0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV6xNEDDvU1? MkP5NlAhdWmw MUTEUXNQyqB? NFTJOHFmdmijbnPld{B{d3KjZnXubYIuKGGwZDDzeY5qfGmwaXKtbY5lfWOnZDDndo94fGhiaX7obYJqfGmxbh?= MkD2NlUxOTN7MEe=
Caki-1 M2DYNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NE\pRYwyOCEEtV2= Moq0NlAhdWmw MnLySG1UV8Li M{n4RoVvcGGwY3XzJJNwemGoZX7pZk0h[W6mIIP1col1cW6rYj3pcoR2[2WmIHfyc5d1cCCrbnjpZol1cW:w MoTINlUxOTN7MEe=
HaCaT  NFzyeVhCeG:ydH;zbZMhSXO|YYm= Ml7TNVAhyrWP MW[yNEBucW5? MXvEUXNQyqB? M2HrXYlv[3KnYYPld{Bxem:yb4L0bY9veyCxZjDhdI9xfG:2aXOgZ4VtdHNiZIXlJJRwKHS{ZXH0cYVvfCC5aYToJJNwemGoZX7pZkBweiC|dX7peIlvcWJ? NF7UVWgzPTBzM{mwOy=>
FHL-primed hNSCs MljnR4VtdCCYaXHibYxqfHliQYPzZZk> MVSwMlAzNTVizszN NYPoR3hTPzJiaB?= NF65XoNt\WGmczD0c{B1cGVibH;zd{Bw\iClZXzsJJZq[WKrbHn0fUBifCCqaXfoJINwdmOnboTyZZRqd25? NHPtTGszPDl2NUSzOC=>
ELL-primed hNSCs MUnD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NWn0SopGOC5yMj21JO69VQ>? NHPpd4Y4OiCq NX6zfYpQdGWjZIOgeI8hfGinIHzvd5Mhd2ZiY3XscEB3cWGkaXzpeJkh[XRiaHnnbEBkd26lZX70doF1cW:w NGfvZYMzPDl2NUSzOC=>
SS NVrCT2dWS2WubDDWbYFjcWyrdImgRZN{[Xl? MX2xMVExKM7:TR?= MoXtO|IhcA>? NGDzZY1FVVOR MWDjZZV{\XNiYTDkc5NmNWSncHXu[IVvfCCrbnjpZol1cW:wIH;mJJRp\SC4aXHibYxqfHoEoB?= MVGyOFc2PjFzMR?=
SeAx  M2X4OmNmdGxiVnnhZoltcXS7IFHzd4F6 NELj[IQyNTFyIN88US=> M2\VNFczKGh? MXzEUXNQ NFixN5Jk[XW|ZYOgZUBld3OnLXTldIVv\GWwdDDpcohq[mm2aX;uJI9nKHSqZTD2bYFjcWyrdIpCpC=> Mn75NlQ4PTZzMUG=
HuT-78 MYDD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MXmxMVExKM7:TR?= NY\LSnJtPzJiaB?= NFHke3dFVVOR MXfjZZV{\XNiYTDkc5NmNWSncHXu[IVvfCCrbnjpZol1cW:wIH;mJJRp\SC4aXHibYxqfHoEoB?= NHqzPXgzPDd3NkGxNS=>
CD4+ NF7PZXpCeG:ydH;zbZMhSXO|YYm= MYCxNQKBkc7:bR?= NV7zcmhkOjRiaB?= NHfTO|VFVVOR M1LCd4lv\HWlZYOgZZBweHSxc3nzJJN1em:wZ3z5 M4GwXVI1PzV4MUGx
MCF-7 NWTTcIJHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M336T|AvPDZ7LUOuO|Uh|ryP M2HFWFUh\A>? MVTy[YR2[2W|IHPlcIwhdnWvYnXyJJNq\26rZnnjZY51dHl? NUXHfoFsOjR5MkiwO|g>
MCF-7/LCC1 MljlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlTUNE41PjlvMz63OUDPxE1? NWOxUpJLPSCm MmnGdoVlfWOnczDj[YxtKG63bXLldkB{cWewaX\pZ4FvfGy7 MnfONlQ4OjhyN{i=
MCF-7/LCC9 NHHJSJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWHyT2xwOC52NkmtN{44PSEQvF2= MYO1JIQ> NHzTdZRz\WS3Y3XzJINmdGxiboXtZoVzKHOrZ37p[olk[W62bIm= NWHwXppZOjR5MkiwO|g>
HaCaT MnvRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4PjSlExKML3TR?= MXSyNEBucW5? MlLMSG1UV8Li M4Hn[IVvcGGwY3XzJIV3\XKxbHnteZMucW6mdXPl[EBk\WyuIHfyc5d1cCCrbnjpZol1cW:w MlnMNlQ1OjNzM{G=
HaCaT M4W3TGFxd3C2b4Ppd{BCe3OjeR?= NWX2OGRROTBiwsXN MmHnNlAhdWmw MnSwSG1UV8Li NITyN|NmdmijbnPld{B1cGViYYDvdJRwfGmlIHXm[oVkfHNib3[g[ZZmem:uaX31dy=> MV[yOFQzOzF|MR?=
MDA-MB-231 NFnnSVlHfW6ldHnvckBCe3OjeR?= MV:xNEDDvU1? M{fmWFI1KGh? NH7oc4FFVVORwrC= MnjXdoVlfWOnczDQMXNVSVR|IHX4dJJme3Orb36= NFzUeVMzPDN5NkW4Oi=>
SUM-159 MnfYSpVv[3Srb36gRZN{[Xl? NEP5ZpQyOCEEtV2= Mn7GNlQhcA>? NYr4fG1sTE2VT9Mg MXzy[YR2[2W|IGCtV3RCXDNiZYjwdoV{e2mxbh?= Mom4NlQ{PzZ3OE[=
SK-BR-3 NW[0[Y11TnWwY4Tpc44hSXO|YYm= NETobmcyOCEEtV2= NY\3bXlROjRiaB?= MVjEUXNQyqB? MVTy[YR2[2W|IGCtV3RCXDNiZYjwdoV{e2mxbh?= NUHLUZVxOjR|N{[1PFY>
MCF7-HER2 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2W4elAuOTBizszN MXy0PEBp Ml;DSG1UVw>? NXOwe|FUcW6mdXPld{Bk\WyuIHTlZZRpKGSxc3Wg[IVx\W6mZX70cJk> M4D5SVI1Ojl5NUC4
MCF7-HER2 M3zXcWZ2dmO2aX;uJGF{e2G7 MVu1JO69VQ>? NX;p[ZhHOjRiaB?= M3vuSmROW09? MUjkbY1qdmm|aHXzJHNwgC1{LDDPZ5QuPCxiYX7kJJNtfWdiZYjwdoV{e2mxbh?= NFPoSo8zPDJ7N{WwPC=>
MCF7-HER2 MlPRSpVv[3Srb36gRZN{[Xl? NUTxeXd4PSEQvF2= NX7wZVlROjRiaB?= M3Hvc2ROW09? NYLuSmN3\GWlcnXhd4V{KHSqZTDlfJBz\XO|aX;uJIxmfmWuczDv[kBGVVRibXHyb4Vzeyxidnnt[Y51cW5iYX7kJJNtfWd? NUPxUWMyOjR{OUe1NFg>
MCF7-HER2 NH;PcopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MonCOUDPxE1? MoDRNlQhcA>? M3KxXGROW09? NWnkTFdn\W6qYX7j[ZMh[2WubDDndo94fGhiaX7obYJqfGmxbjDjc41jcW6nZDD3bZRpKEincnPldJRqdg>? M3vjdlI1Ojl5NUC4
HMECs  MoKwSpVv[3Srb36gRZN{[Xl? MUSxNEDPxE1? Mn65NkBp M{fGcYlvcGmkaYTzJGlHVs7zwrDt[YRq[XSnZDDwbI9{eGixconsZZRqd25ib3[gV3RCXDFuIGPURXQzKGGwZDDTWGFVOw>? MX:yOFIyOTN{Nx?=
HTR8/SVneo M3rrUWZ2dmO2aX;uJGF{e2G7 Ml;KNeKh|ryPwrC= Mmj2NeKhcA>? NGjKVm1{fXCycnXzd4VlKE:VTT3pcoR2[2WmIGPURXQ{KHCqb4PwbI9zgWyjdHnvci=> NV3oWW5uOjRyNkCyOFE>
HTR8/SVneo MoKxSpVv[3Srb36gRZN{[Xl? NIDaUW4xNjVxMdMg{txOyqB? NXTSZnh7PDhiaB?= M3W4epJme3SxcnXzJJRp\SCneIDy[ZN{cW:wIH;mJGUu[2GmaHXybY4he3WycILld5Nm\CCkeTDPV20> MoPxNlQxPjB{NEG=
HTR8/SVneo M3PlbGZ2dmO2aX;uJGF{e2G7 NX;VOmU2OcLizszNxsA> NIDuZ5c1QCCq MlTGd4lodmmoaXPhcpRtgSCrbnPy[YF{\XNibXnndoF1cW:wIHL5JG9UVcLi NUTNSINLOjRyNkCyOFE>
C13* NFvMUHRCeG:ydH;zbZMhSXO|YYm= NGO3V3gxNTFyIN88US=> Mn3iNlQwPDhiaB?= NE\6NlhqdmS3Y3XzJIFxd3C2b4Ppd{BqdiCjIHTvd4Uh[W6mIITpcYUh\GWyZX7k[Y51KG2jbn7ldi=> NWrLenNTOjN7NkK1OVg>
OV2008 M3HIVmFxd3C2b4Ppd{BCe3OjeR?= M2TNNlAuOTBizszN MlfMNlQwPDhiaB?= MmjnbY5lfWOnczDhdI9xfG:|aYOgbY4h[SCmb4PlJIFv\CC2aX3lJIRmeGWwZHXueEBu[W6wZYK= MnTJNlM6PjJ3NUi=
C13* NHzMUolCeG:ydH;zbZMhSXO|YYm= NYDjbGxOOjRxNEigbC=> NHrqe3RmdmijbnPld{BkcXOybHH0bY4ucW6mdXPl[EBieG:ydH;zbZM> NF;nXG0zOzl4MkW1PC=>
OV2008 MlO4RZBweHSxc3nzJGF{e2G7 MoDuNlQwPDhiaB?= Mmm3[Y5p[W6lZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZZBweHSxc3nz NIL6eFQzOzl4MkW1PC=>
W480  MVHGeY5kfGmxbjDBd5NigQ>? NVn1UVZNOi53L{GwJO69VQ>? M{HpblMxKG2rbh?= Mn74SG1UVw>? NG[2ZZd{\W6|aYTpfoV{KGOnbHzzJJRwKGOqZX3vdoFlcW:2aHXyZZB6KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz M2LHb|I{QTN2OUey
SW837 NYKybHdLTnWwY4Tpc44hSXO|YYm= MnTHNk42NzFyIN88US=> MVSzNEBucW5? MYPEUXNQ MlLqd4Vve2m2aYrld{Bk\WyuczD0c{BkcGWvb4Lh[IlwfGincnHwfUBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MYWyN|k{PDl5Mh?=
T24 M1TX[mZ2dmO2aX;uJGF{e2G7 M3nEeVIwOTBxMkCg{txO MlXjNlQhcA>? NIDsWnFk[XW|ZYOg[I9{\S2mZYDlcoRmdnRiaX7obYJqfGmxbjDv[kB1cGViQ2jDUFEzNWmwZIXj[YQhcW6lcnXhd4Uhd2ZiaX72ZYRqdmdiY3XscJM> MYWyN|UzPjB5OR?=
CNE1 NGPGXYhHfW6ldHnvckBCe3OjeR?= M4nYZlIxKML3TR?= NIX1bIk1QCCq NGWyfmJjdG:la4OgeIhmKEmOLU[gbY5kemWjc3XkJJBpd3OyaH;yfYxifGmxbjDv[kBUfGG2Mx?= NYfnbXV6OjN|OEK5NVQ>
CNE2 M{nFTGZ2dmO2aX;uJGF{e2G7 MlvpNlAhyrWP MWm0PEBp NFrWVpljdG:la4OgeIhmKEmOLU[gbY5kemWjc3XkJJBpd3OyaH;yfYxifGmxbjDv[kBUfGG2Mx?= NX3BTI92OjN|OEK5NVQ>
HONE1 NUTCUHZ3TnWwY4Tpc44hSXO|YYm= NGjJSJEzOCEEtV2= Mn3JOFghcA>? NU\STY1S[myxY3vzJJRp\SCLTD22JIlv[3KnYYPl[EBxcG:|cHjvdplt[XSrb36gc4YhW3SjdEO= NXf3e2d1OjN|OEK5NVQ>
CNE1 M37sfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX\Ic|lsPCEQvF2= MmC3d4lodmmoaXPhcpRtgSC{ZXT1Z4V{KGOnbHygeoli[mmuaYT5 MmfMNlM{QDJ7MUS=
CNE1 NG\Ffo5HfW6ldHnvckBCe3OjeR?= Mn;jNE0zOCEQvF2= Ml30NE01KGh? Mn7xbY5pcWKrdIOgV5RifDNiYXP0bZZifGmxbjDpckBiKGSxc3WtJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK= NF3OPZEzOzN6MkmxOC=>
CNE2 M1[yVWZ2dmO2aX;uJGF{e2G7 NELqR4kxNTJyIN88US=> MmLXNE01KGh? MnHCbY5pcWKrdIOgV5RifDNiYXP0bZZifGmxbjDpckBiKGSxc3WtJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK= NXPzOnp6OjN|OEK5NVQ>
HONE1 MXHGeY5kfGmxbjDBd5NigQ>? MYWwMVIxKM7:TR?= NXXKSYFEOC12IHi= M13lNYlvcGmkaYTzJHN1[XR|IHHjeIl3[XSrb36gbY4h[SCmb4PlMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy MWWyN|M5OjlzNB?=
CNE1 M{PWOGNmdGxiVnnhZoltcXS7IFHzd4F6 MknCNE42NTZ2IN88US=> MUm0PEBp MUHzeZBxemW|c3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT2gZY5lKHSrbXWt[IVx\W6mZX70JI1idm6nch?= NVHSV5BNOjN|OEK5NVQ>
CNE2 MWHD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MVWwMlUuPjRizszN MYW0PEBp NVnC[2Yze3WycILld5NmeyClZXzsJJZq[WKrbHn0fUBqdiCjIHTvd4UuKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> MmW4NlM{QDJ7MUS=
HONE1 NYTKT4ZIS2WubDDWbYFjcWyrdImgRZN{[Xl? NFrRcGwxNjVvNkSg{txO NUnNcll6PDhiaB?= NILae4p{fXCycnXzd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> MYqyN|M5OjlzNB?=
C666-1  NIXzSXVE\WyuIG\pZYJqdGm2eTDBd5NigQ>? Ml3nNE42NTZ2IN88US=> NGPtZXI1QCCq Mnn0d5VxeHKnc4Pld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3WtJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK= NHTZR40zOzN6MkmxOC=>
CNE1 NVLwVZVvSXCxcITvd4l{KEG|c3H5 NXTJUZF2OTBiwsXNxsA> M2jaU|Q5KGh? M4DPe4lv\HWlZYOgZZBweHSxc3nzxsA> NV34V41bOjN|OEK5NVQ>
CNE2 MWXBdI9xfG:|aYOgRZN{[Xl? MmWwNVAhyrWPwrC= M3K3[VQ5KGh? NH2zbG1qdmS3Y3XzJIFxd3C2b4Ppd:Kh MViyN|M5OjlzNB?=
HONE1 M1P0dmFxd3C2b4Ppd{BCe3OjeR?= M2PUZVExKML3TdMg NWHFdFJSPDhiaB?= MWLpcoR2[2W|IHHwc5B1d3Orc9Mg MoGxNlM{QDJ7MUS=
CNE2 Mlv4R4VtdCCYaXHibYxqfHliQYPzZZk> M1PXdVEwOiEQvF2= NGXnUVU1QCCq NYjJ[lFOe2Wwc3n0bZpmKGOnbHzzJJRwKHKjZHnveIhmemGyeR?= NYjMeZZOOjN|OEK5NVQ>
HONE1 NWXBeYlWS2WubDDWbYFjcWyrdImgRZN{[Xl? M1zNUlEwOiEQvF2= NV:yVmNOPDhiaB?= M2fYcZNmdnOrdHn6[UBk\WyuczD0c{Bz[WSrb4To[ZJieHl? NGL3NXQzOzN6MkmxOC=>
C666-1  M1LueWNmdGxiVnnhZoltcXS7IFHzd4F6 NU\CWldROS9{IN88US=> NGnYZ|c1QCCq M1TBfJNmdnOrdHn6[UBk\WyuczD0c{Bz[WSrb4To[ZJieHl? MUKyN|M5OjlzNB?=
HEC-1A M3fvW2Z2dmO2aX;uJGF{e2G7 MUGxxsDPxE4EoB?= M4T6blI1KGh? M4PsW2ROW09? NULiSZI{[myxY3vzJJRp\SCPVVOyNE1mdmijbnPl[EBqdn[jc3nvckB1emmpZ3Xy[YQh[nliMUClJGZDWw>? NIC0O2EzOzJ4MkKwPC=>
RL95-2 MkTVSpVv[3Srb36gRZN{[Xl? MVyxxsDPxE4EoB?= NW\1eHdOOjRiaB?= NHf6WIlFVVOR NELkWZFjdG:la4OgeIhmKE2XQ{KwMYVvcGGwY3XkJIlvfmG|aX;uJJRzcWepZYLl[EBjgSBzMDWgSmJU MnPPNlMzPjJ{MEi=
HEC-1A MkX0SpVv[3Srb36gRZN{[Xl? MYGxxsDPxE4EoB?= MUCyOEBp MYrEUXNQ NV7rW4d5[myxY3vzJJRp\SCPVVOyNE1mdmijbnPl[EBqdn[jc3nvckB1emmpZ3Xy[YQh[nliRVfGxsA> NF7zcGEzOzJ4MkKwPC=>
RL95-2 MkPGSpVv[3Srb36gRZN{[Xl? NFnz[|EyyqEQvF5CpC=> MYeyOEBp MV;EUXNQ MkjyZoxw[2u|IITo[UBOXUN{MD3lcohidmOnZDDpcpZie2mxbjD0dolo\2W{ZXSgZpkhTUeIwrC= MnTjNlMzPjJ{MEi=
CT26 MWTGeY5kfGmxbjDBd5NigQ>? NYjoe|c4OjBibV2= NVXDc3I3OSCq MoLId5VxeHKnc4Pld{BJT0ZvaX7keYNm\CCYRVfGJIV5eHKnc4Ppc47DqA>? NXT5SIFSOjN{M{OxOlM>
UM-SCC-17B NUTTVZF{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVjOfWZ5UUN3ME2yMlU3OiEEsTCwMlQxQSEQvF2sJGdKPTB;MT6yO|khyrFiMD6xPVQh|ryP MoPHNlI4PzB6OUm=
OSC-19 NVzyUFFKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M13tVGlEPTB;Mz60PFEhyrFiMD65OVMh|ryPLDDHTVUxRTFwM{[2JOKyKDBwN{ewJO69VQ>? NV7MdmtbOjJ5N{C4PVk>
Cal33 M3zGcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1y3TWlEPTB;Mj6yPFIhyrFiMD60NlMh|ryPLDDHTVUxRTFwM{S5JOKyKDBwM{[zJO69VQ>? NVjJOGU2OjJ5N{C4PVk>
UM-SCC-22B M1LURmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LjSmlEPTB;Mj62OFghyrFiMD61OFIh|ryPLDDHTVUxRTFwM{KwJOKyKDBwMkC0JO69VQ>? MUmyNlc4ODh7OR?=
UM-SCC-17B MnnQSpVv[3Srb36gRZN{[Xl? NVq3N44zOC1|MDFOwG0> MkLHNE0zPCCq NHr2WXpqdmirYnn0d{BUXEGWMzDhZ5RqfmG2aX;uJIRwe2ViYX7kJJRqdWViZHXw[Y5l\W62bIm= NVfYUWdnOjJ5N{C4PVk>
OSC-19 MoHUSpVv[3Srb36gRZN{[Xl? M2jVTFAuOzBizszN MUWwMVI1KGh? MVrpcohq[mm2czDTWGFVOyCjY4TpeoF1cW:wIHTvd4Uh[W6mIITpcYUh\GWyZX7k[Y51dHl? NHLmTZUzOjd5MEi5PS=>
Cal33 M3O1OmZ2dmO2aX;uJGF{e2G7 MnG3NE0{OCEQvF2= MYmwMVI1KGh? M1vtV4lvcGmkaYTzJHNVSVR|IHHjeIl3[XSrb36g[I9{\SCjbnSgeIlu\SCmZYDlcoRmdnSueR?= MmjGNlI4PzB6OUm=
UM-SCC-22B M1rReGZ2dmO2aX;uJGF{e2G7 MU[wMVMxKM7:TR?= MWGwMVI1KGh? NHPIdWhqdmirYnn0d{BUXEGWMzDhZ5RqfmG2aX;uJIRwe2ViYX7kJJRqdWViZHXw[Y5l\W62bIm= NH\G[4kzOjd5MEi5PS=>
U-87MG NWn5SHZ5S2WubDDWbYFjcWyrdImgRZN{[Xl? MkPnNE0yOCEQvF2= MUO3NkBp MXTEUXNQ NGq3XoVqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 Ml72NlU1OzZ4OEK=
U-373MG M3exeWNmdGxiVnnhZoltcXS7IFHzd4F6 MlXDNE0yOCEQvF2= MnzqO|IhcA>? NV7qbXpQTE2VTx?= Mn23bY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? NIq4dI0zPTR|Nk[4Ni=>
SH-SY5Y MULD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NIHWPG4xNTFyIN88US=> MmTqO|IhcA>? Mn3RSG1UVw>? MkjwbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? MWSyOVQ{PjZ6Mh?=
Tu-9648 MnTUR4VtdCCYaXHibYxqfHliQYPzZZk> MlTsNE0yOCEQvF2= MXe3NkBp M2LBemROW09? MVXpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 M3jvSVI2PDN4Nkiy
Neuro-2a M2G3VGNmdGxiVnnhZoltcXS7IFHzd4F6 NFLCRpoxNTFyIN88US=> MVG3NkBp NVr4NIhwTE2VTx?= MlTvbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? MUeyOVQ{PjZ6Mh?=
PCNs NH;GT41E\WyuIG\pZYJqdGm2eTDBd5NigQ>? MYqwMVExKM7:TR?= Mk\aO|IhcA>? M13GbmROW09? MYPpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 NEL1[oczPTR|Nk[4Ni=>
PGCs NV7FfYNlS2WubDDWbYFjcWyrdImgRZN{[Xl? MYSwMVExKM7:TR?= MXS3NkBp M2\ROGROW09? MUXpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 NUnFOpNnOjV2M{[2PFI>
RAW264.7 M2e5VGZ2dmO2aX;uJGF{e2G7 NVr6R3NNOTBizszN NIf1UXoyOiCq MYfhZpJw\2G2ZYOgeIhmKG2UTlGg[ZhxemW|c3nvcpMhd2ZiSlHLNkwhW1SDVEGsJHNVSVR{LDDhcoQhW1SDVEOgbY5lfWOnZDDifUBFV05iYX7kJHQuOiC2b4jpci=> NYS0Vm1iOjJ2NUS0N|E>
RAW264.7 NFLW[GxCeG:ydH;zbZMhSXO|YYm= MmPLOU8yOCEQvF2= M2DIZ|Q2KG2rbh?= MlXU[Y5p[W6lZYOgeI95cW6|IHnu[JVk\WRiYYDvdJRwe2m|IHHu[EBOVVBibH;zdy=> MXSyNlQ2PDR|MR?=
SW480 NF61V|RE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NG\ifog2NzFyL{KwJO69VQ>? MWC3NkBp MUDpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCxZjD0bIUhSUyGSDuvR2QyOzNtwrDj[Yxtew>? M{T2TVIyQTByM{m3
HCT116 Mli0R4VtdCCYaXHibYxqfHliQYPzZZk> M{TyfFUwOTBxMkCg{txO MoTUO|IhcA>? M3jQZYlvcGmkaYTzJINmdGxidnnhZoltcXS7IH;mJJRp\SCDTFTIL{9ETDF|MzxCpINmdGy| NVL1VYt4OjF7MECzPVc>
DLD-1  MkL4R4VtdCCYaXHibYxqfHliQYPzZZk> NEfrfJI2NzFyL{KwJO69VQ>? MnrQO|IhcA>? MkHEbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHlib3[geIhmKEGORFirM2NFOTN|K9MgZ4VtdHN? M4PiVVIyQTByM{m3
SNU387  MVzD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NGnJOlMzOMLizszNxsA> Ml;tNlQhcA>? MXjy[YR2[2W|IHPlcIwhfmmjYnnsbZR6 Mkn6NlE{OTF7N{W=
SNU398 NEnPZ3dE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NEHvdGUzOMLizszNxsA> NV31dVFVOjRiaB?= M3zneZJm\HWlZYOgZ4VtdCC4aXHibYxqfHl? M2qxclIyOzFzOUe1
HepG2 MnTHR4VtdCCYaXHibYxqfHliQYPzZZk> MoH6NlDDqM7:TdMg NH7qR4ozPCCq NGT3WmVz\WS3Y3XzJINmdGxidnnhZoltcXS7 MUiyNVMyOTl5NR?=
Huh-7 MVLD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NYjqR2NCOjEEoN88UeKh MX2yOEBp MUHy[YR2[2W|IHPlcIwhfmmjYnnsbZR6 M2DV[FIyOzFzOUe1
VSMC NIGwcVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmfEN{82NzFyIN88US=> M{\TUVMxKG2rbh?= M4nTe2ROW09? M3LDSpBz\X[nboTzJHBFT0ZvIHHu[EB1cHKxbXLpck1u\WSrYYTl[EBXW02FIIDyc4xq\mW{YYTpc44hcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> MYOyNFg1PzNyNh?=
MDA-MB-231 NULhW4E6SXCxcITvd4l{KEG|c3H5 M2O1fFExKM7:TR?= MUKyOEBp NGTJTZJFVVORwrC= NV3mNpJGcW6mdXPld{BieG:ydH;zbZM> NYG5cYhTOTdzMUSwNFU>
MDA-MB-435S NF3KbYtCeG:ydH;zbZMhSXO|YYm= M{PoNVExKM7:TR?= NFKyeFAzPCCq NVHYSJJvTE2VT9Mg M2DaWYlv\HWlZYOgZZBweHSxc3nz M2PJbFE4OTF2MEC1

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-STAT3 / STAT3 ; 

PubMed: 25261365     


(A) PC3M-1E8 cells were treated with different doses of Stattic for 4 h. (B) PC3M-1E8 cells were treated with 10 μM Stattic for different time points. 

Survivin / c-Myc / Bcl-xl ; 

PubMed: 25261365     


Western blot analysis of STAT3 downstream targets in PC3M-1E8 cells treated with 10 μM Stattic for 24 h. 

PARP / C-PARP / Caspase-3 / C-Caspse-3 ; 

PubMed: 23382914     


NPC cells were exposed to the indicated concentrations of Stattic for 48 h; apoptotic cells were measured by western blot analysis of cleaved PARP and cleaved caspase-3. Protein levels were quantified using ImageJ software. 

25261365 23382914
Immunofluorescence
p-STAT3 / STAT3 / Survivin ; 

PubMed: 25261365     


Cellular localization of pSTAT3 and survivin (green) was observed by confocal microscopy after immunofluorescent staining of PC3M-1E8 cancer cells treated with 10 μM Stattic.

25261365
Growth inhibition assay
Cell viability; 

PubMed: 23382914     


Stattic inhibits the cell viability of NPC cells in a time-dependent manner. CNE2 cells were treated with 8 µM Stattic for the indicated times, and cell viability was measured by the MTT assay. 

23382914
ELISA
BDNF; 

PubMed: 27456333     


BDNF secretion was analyzed using ELISA in A549 and H1299 cells treated with or without Stattic. Results are shown as the mean ± S.E. n = 3; *p < 0.05, vs Control group, Student’s t-test. 

27456333

Protocol

Kinase Assay:

[1]

+ Expand

High-Throughput Screening and FluorescencePolarization Assays:

Screening is performed at approximately 30 °C. The specificity ofscreening hits is validated in analogous assays for binding of thetest compounds to the SH2 domains of STAT1, STAT5, and Lck. The final concentration of buffer components used for all FP assays is 10 mM HEPES (pH 7.5), 1 mM EDTA, 0.1% Nonidet P-40, 50 mM NaCl, and 10% DMSO. The absence of dithiothreitol is essential for inhibitory activity. The sequences of the peptides are: STAT3, 5-carboxyfluorescein-GY(PO3H2)LPQTV-NH2; STAT1, 5-carboxyfluorescein-GY(PO3H2)DKPHVL; STAT5, 5-carboxyfluorescein-GY(PO3H2)LVLDKW; and Lck, 5-carboxyfluorescein-GY(PO3H2)EEIP. For specificity analysis at 30 °C, proteins are used at 150 nM (STAT1, STAT3, and STAT5). For specificity analysis at 37 °C, proteins are used at 370 nM (STAT3) or 100 nM (Lck). Proteins are incubated with test compounds in Eppendorf tubes at the indicated temperatures for 60 min prior addition of therespective 5-carboxyfluorescein labeled peptides (final concentration: 10 nM).Before measurement at room temperature, the mixtures are allowed to equilibrate for at least 30 min. Test compounds are used at the indicated concentrations diluted from a 20× stock in DMSO. Binding curves and inhibition curves are fitted with SigmaPlot. All competition curves are repeated three times in independent experiment
Cell Research:

[2]

+ Expand
  • Cell lines: Ly3 cells
  • Concentrations: ~2.5 μM
  • Incubation Time: 48 h
  • Method:

    MTS


    (Only for Reference)
Animal Research:

[2]

+ Expand
  • Animal Models: Ly3 parental NOD/SCID IL2R null xenograft
  • Formulation: --
  • Dosages: 3.75 mg/kg every day
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 42 mg/mL (198.87 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+H2O
For best results, use promptly after mixing.
8mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 211.19
Formula

C8H5NO4S

CAS No. 19983-44-9
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    We need to dissolve the compound for i.p. injection, could you give me some suggestions?

  • Answer:

    S7024 Stattic can be dissolved in 2% DMSO/PEG 300/dd H2O at 8 mg/mL as a clear solution.

STAT Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID