Stattic

Catalog No.S7024

For research use only.

Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1. Stattic induces apoptosis.

Stattic Chemical Structure

CAS No. 19983-44-9

Selleck's Stattic has been cited by 189 publications

Purity & Quality Control

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Biological Activity

Description Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1. Stattic induces apoptosis.
Features Stattic is the first non-peptide small molecule with inhibitory activity against STAT3 SH2 domain regardless of the STAT3 phosphorylation state in vitro.
Targets
STAT3 [1]
(Cell-free assay)
5.1 μM
In vitro

Stattic inhibits binding of a phosphotyrosine-containing peptide derived from the gp130 receptor to the STAT3 SH2 domain in a strongly temperature-dependent manner. Stattic only has a very weak effect on binding of a tyrosinephosphorylated peptide to the SH2-domain of the tyrosine kinase Lck. And it doesn’t inhibit dimerization of two other dimeric transcription factors (c-Myc/Max and Jun/Jun). It also inhibits fluorescein-labeled phosphopeptides to the SH2 domains of STAT1 and STAT5b. Stattic selectively inhibits DNA binding of STAT3 homodimers at a concentration of 10 μM. Shown to inhibit cellular phosphorylation of STAT3 at Tyr705 with little effect towards STAT1 phosphorylation at Tyr701 (in HepG2 cells) or the phosphorylation of JAK1, JAK2, and c-Src (in MDA-MB-231 and MDA-MB-235S cells). Stattic increases the apoptotic rate of STAT3-dependent breast cancer cell lines. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H9c2 NX7tbnBoTnWwY4Tpc44hSXO|YYm= M1jzblEx6oDLzszN NFiy[Hg16oDLaB?= MV;y[ZZmenOnczD0bIUh\W[oZXP0d{Bw\iCLTD2yOy=> MlrIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|M{m2N|MoRjJ4M{O5OlM{RC:jPh?=
NPC  M1TSXWZ2dmO2aX;uJGF{e2G7 M4TqSFAuPy53INM1US=> MUjhZo9tcXOqZYOgSW1VNWyra3WgcY9t\WO3bHHyJIFtfGW{YYTpc45{NCCjbnSgZ4VtdCCvaXfyZZRqd25iYX7kJIlvfmG|aX;uJIlv\HWlZXSgZpkhWkuLUDDrco9kc2Sxd36= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTlzNUSzNEc,OjV7MUW0N|A9N2F-
HASMC NHm4OYVHfW6ldHnvckBCe3OjeR?= Mn7qNU4zPS13IN88US=> NGPjVVczOCCvaX6= NIe0fphFVVORwrC= M3PwS4lvcGmkaYTzJJAuMFlrLWPURXQuOSx|LEWgd4lodmGuc9Mg NXTCVIw1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4OFk3OjJpPkK1PFQ6PjJ{PD;hQi=>
H9c2  NV3KO2JDTnWwY4Tpc44hSXO|YYm= MXSyM|ExKM7:TR?= M{frb|IhcA>? NFv2elFFVVORwrC= MXXhZpJw\2G2ZYRCpJRp\SCleYTvdJJwfGWldHn2[UBm\m[nY4TzJI9nKEmOLUK3JIFo[Wmwc4SgV2g> M2DIWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEKwPVA4Lz5{NUiyNFkxPzxxYU6=
A431 M4jFfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF\QUokzyqEQvF2= MWiyxsBp MorPZoxw[2u|IFXHSk1z\X[ncoPl[EBl\WO{ZXHz[ZMhcW5iY3XscEB3cWGkaXzpeJk> M3zqb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{KwOFM2Lz5{NUeyNFQ{PTxxYU6=
A431 M4WxZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX2yxsDPxE1? MmjFNuKhcA>? MV;pcoNz\WG|ZYOgbY4h[XCxcITvd4l{KGmwZIXj[YQh[nlic3jpb49vcW5? NIPYdog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUeyNFQ{PSd-MkW3NlA1OzV:L3G+
SiHa NECxWY9E\WyuIG\pZYJqdGm2eTDBd5NigQ>? MUK1MVc2KG6P M3XUWVI1yqCqwrC= M2HnSpNpd3e|IH3vdpBpd2yxZ4mgc4Yh[SC2eYDpZ4FtKGGyb4D0c5Rq[yClZXzsJIFv\CCmb4PlMYRmeGWwZHXueEBtd3O|IH;mJINmdGxidnnhZoltcXS7wrC= M1;xcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUO5OlQ1Lz5{NUWzPVY1PDxxYU6=
SiHa NX;FempSTnWwY4Tpc44hSXO|YYm= NX;lcnA3PS15NTDuUS=> MoH6NlTDqGkEoB?= NVHUcYU5emWmdXPld{B1cGYEoIDoc5NxcG:{eXzheIlwdiCjdDD0bIUhfHm{b4PpcoUhemW|aXT1[UA4ODYEoB?= MlHMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3M{m2OFQoRjJ3NUO5OlQ1RC:jPh?=
ECA109 M{P4[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFzmelMxNTJyIN88US=> NHTLSGczPMLiaNMg NGG3cmZKSzVyPUWuOVAh|ryP MnrxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2OUK0PFAoRjJ3NEmyOFgxRC:jPh?=
TE13 M3\zeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlPFNE0zOCEQvF2= NYL4eYprOjUEoHlCpC=> M{TWTGlEPTB;Nj6xOeKh|ryP NYH5b3drRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0PVI1QDBpPkK1OFkzPDhyPD;hQi=>
KYSE150  M33XcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUfLflV1OC1{MDFOwG0> NVflXmZJOjUEoHlCpC=> Mle3TWM2OD1zMj62OOKh|ryP M1LmfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEmyOFgxLz5{NUS5NlQ5ODxxYU6=
ECA109 MWDDcI9vd2enbnnjJHN2en[rdnHsJGF{e2G7 MlPENE42yqEQvF2= MVGyOOKhcMLi MVjzeZBxemW|c3XzJJRp\SClbH;uc4dmdmmlIH\vdo1ifGmxbh?= M1jpbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEmyOFgxLz5{NUS5NlQ5ODxxYU6=
TE13 NHzyZpFEdG:wb3flcolkKFO3co\peoFtKEG|c3H5 NWTSVmRyOC53wrFOwG0> NGDiZo0zPMLiaNMg MoS5d5VxeHKnc4Pld{B1cGViY3zvco9o\W6rYzDmc5Ju[XSrb36= MlHOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2OUK0PFAoRjJ3NEmyOFgxRC:jPh?=
KYSE150  MnjUR4xwdm:pZX7pZ{BUfXK4aY\hcEBCe3OjeR?= M4rnclAvPcLizszN Mkf2NlTDqGkEoB?= NUWwTZl4e3WycILld5NmeyC2aHWgZ4xwdm:pZX7pZ{Bnd3KvYYTpc44> NIXBbW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS5NlQ5OCd-MkW0PVI1QDB:L3G+
ECA109 NVT6b|E5TnWwY4Tpc44hSXO|YYm= NULz[3Q2OC53wrFOwG0> MWSyOOKhcMLi Mor1[Y5p[W6lZYOgTXIucW6mdXPl[EBo\W6ncnH0bY9vKG:oIFTTRpM> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR7MkS4NEc,OjV2OUK0PFA9N2F-
PC3M-1E8 M331V2Z2dmO2aX;uJGF{e2G7 M{f0TVIvPS93L{GwJO69VQ>? MUiwMVQhcA>? Mn3DbY5pcWKrdIOgeIhmKFOWQWSzJIFkfGm4YYTpc44hcW5iYTDkc5NmNSCjbnSgeIlu\S2mZYDlcoRmdnRibXHucoVzyqB? NGHPdFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK2NVM3PSd-MkWyOlE{PjV:L3G+
PC3M-1E8 NU\nVpU1TnWwY4Tpc44hSXO|YYm= NVH5OGdwOTBizszN M2X0bVI1KGh? M{fleoRwf26{ZXf1cIF1\XNiQnPsMZhNNCC|dYL2bZZqdiCjbnSgZ{1OgWN? NH;EfpA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK2NVM3PSd-MkWyOlE{PjV:L3G+
PC3M-1E8 NULWbIc3TnWwY4Tpc44hSXO|YYm= MlzYNVAh|ryP MluyNlQhcA>? NXq0WJJxcW6qaXLpeJMhUUxvNjDpcoR2[2WmIGPURXQ{KGGldHn2ZZRqd25iYX7kJJRp\SCLTD22MYlv\HWlZXSgV3RCXDNiYXP0bZZifGmxbh?= M3zvbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Mk[xN|Y2Lz5{NUK2NVM3PTxxYU6=
PC3M-1E8 MWHDcI9vd2enbnnjJHN2en[rdnHsJGF{e2G7 MlXqNk42NzVxMUCg{txO MlPPbY5pcWKrdIRCpJRp\SClb3zvcpkh\m:{bXH0bY9vKHOrZ37p[olk[W62bIm= M1W2NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Mk[xN|Y2Lz5{NUK2NVM3PTxxYU6=
MDA-MB-231 Mor0SpVv[3Srb36gRZN{[Xl? NIHI[2ozOCEQvF2= NV;neJdnOiCq NE\DUnpmgGirYnn0d{BUdmGrbDDhcoQhTS2lYXTo[ZJqdiCneIDy[ZN{cW:w MmnUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzNUOzOFkoRjJ3MUWzN|Q6RC:jPh?=
H9c2 NVjGeYJ4TnWwY4Tpc44hSXO|YYm= NYjTdm1KOjBiwsXN Mnm3N|AhdWmw MUPEUXNQyqB? MmLyZYJwdGm|aHXzJJBzd3CxZn;sMYlv\HWlZXSgRWtVKHCqb4PwbI9zgWyjdHnvckBifCCkb4ToJJNmejR5MzDhcoQhfGi{M{C4 Ml;2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMEWwOlcoRjJ3MUC1NFY4RC:jPh?=
HaCaT  NV:xd|hwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4LVcVExKML3TR?= NVHPOnNxOjBibXnu MnXaSG1UV8Li NETPN2lmdmijbnPld{B{d3KjZnXubYIuKGGwZDDzeY5qfGmwaXKtbY5lfWOnZDDndo94fGhiaX7obYJqfGmxbh?= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTBzM{mwO{c,OjVyMUO5NFc9N2F-
Caki-1 M3e3Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVrZZ5NJOTBiwsXN MmK3NlAhdWmw M4\PVWROW00EoB?= MVPlcohidmOnczDzc5Ji\mWwaXKtJIFv\CC|dX7peIlvcWJvaX7keYNm\CCpcn;3eIghcW6qaXLpeIlwdg>? NVP5UYg{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwNVM6ODdpPkK1NFE{QTB5PD;hQi=>
HaCaT  MkHURZBweHSxc3nzJGF{e2G7 NIG2fJUyOCEEtV2= NIrtUYUzOCCvaX6= NFrqTlFFVVORwrC= M{\CTolv[3KnYYPld{Bxem:yb4L0bY9veyCxZjDhdI9xfG:2aXOgZ4VtdHNiZIXlJJRwKHS{ZXH0cYVvfCC5aYToJJNwemGoZX7pZkBweiC|dX7peIlvcWJ? NVTremxXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwNVM6ODdpPkK1NFE{QTB5PD;hQi=>
FHL-primed hNSCs MYfD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NEjIbngxNjB{LUWg{txO Mle3O|IhcA>? M1XQSIxm[WS|IITvJJRp\SCub4PzJI9nKGOnbHygeoli[mmuaYT5JIF1KGirZ3igZ49v[2WwdILheIlwdg>? NX62fmoyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OFU1OzRpPkK0PVQ2PDN2PD;hQi=>
ELL-primed hNSCs Mmi0R4VtdCCYaXHibYxqfHliQYPzZZk> NX7wTmE4OC5yMj21JO69VQ>? M2Oze|czKGh? NYXVcXVsdGWjZIOgeI8hfGinIHzvd5Mhd2ZiY3XscEB3cWGkaXzpeJkh[XRiaHnnbEBkd26lZX70doF1cW:w NVvrb2tQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OFU1OzRpPkK0PVQ2PDN2PD;hQi=>
SS NGfGSo5E\WyuIG\pZYJqdGm2eTDBd5NigQ>? NInZbmMyNTFyIN88US=> MYe3NkBp MmW1SG1UVw>? NWftcJpH[2G3c3XzJIEh\G:|ZT3k[ZBmdmSnboSgbY5pcWKrdHnvckBw\iC2aHWgeoli[mmuaYT5xsA> M1HlV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{W2NVEyLz5{NEe1OlEyOTxxYU6=
SeAx  MkLDR4VtdCCYaXHibYxqfHliQYPzZZk> MXuxMVExKM7:TR?= MUe3NkBp NYT4V5RjTE2VTx?= MmrPZ4F2e2W|IHGg[I9{\S2mZYDlcoRmdnRiaX7obYJqfGmxbjDv[kB1cGVidnnhZoltcXS7wrC= NF7NfYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe1OlEyOSd-MkS3OVYyOTF:L3G+
HuT-78 NYnad4h2S2WubDDWbYFjcWyrdImgRZN{[Xl? M3HodVEuOTBizszN MYi3NkBp NVTvZYRbTE2VTx?= MXzjZZV{\XNiYTDkc5NmNWSncHXu[IVvfCCrbnjpZol1cW:wIH;mJJRp\SC4aXHibYxqfHoEoB?= MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd3NkGxNUc,OjR5NU[xNVE9N2F-
CD4+ NYD6TVNWSXCxcITvd4l{KEG|c3H5 NIji[4MyOOLCid88cS=> NXvRUnJZOjRiaB?= NXfLW5d{TE2VTx?= NY\hdmlRcW6mdXPld{BieG:ydH;zbZMhe3S{b37ncJk> NX;5SW17RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3OVYyOTFpPkK0O|U3OTFzPD;hQi=>
MCF-7 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlTBNE41PjlvMz63OUDPxE1? M3nLOlUh\A>? NV7DR3pHemWmdXPld{Bk\WyuIH71cYJmeiC|aXfubYZq[2GwdHz5 Ml;WQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5MkiwO|goRjJ2N{K4NFc5RC:jPh?=
MCF-7/LCC1 M3fNU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MliwNE41PjlvMz63OUDPxE1? MUi1JIQ> M{LWdZJm\HWlZYOgZ4VtdCCwdX3i[ZIhe2mpbnnmbYNidnSueR?= M4fwUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{K4NFc5Lz5{NEeyPFA4QDxxYU6=
MCF-7/LCC9 M3Swbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlG5NE41PjlvMz63OUDPxE1? M{DxZlUh\A>? NI\RNpBz\WS3Y3XzJINmdGxiboXtZoVzKHOrZ37p[olk[W62bIm= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd{OEC3PEc,OjR5MkiwO|g9N2F-
HaCaT MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1nNZ|ExKML3TR?= MmDMNlAhdWmw NULuU4R5TE2VT9Mg NVXSNmVW\W6qYX7j[ZMh\X[ncn;sbY12ey2rbnT1Z4VlKGOnbHyg[5Jwf3SqIHnubIljcXSrb36= NXvxU5F6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0NlMyOzFpPkK0OFI{OTNzPD;hQi=>
HaCaT MnXkRZBweHSxc3nzJGF{e2G7 MmG1NVAhyrWP MX2yNEBucW5? MXLEUXNQyqB? MWjlcohidmOnczD0bIUh[XCxcITveIlkKGWoZnXjeJMhd2ZiZY\ldo9tcW23cx?= NFvVPVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NESyN|E{OSd-MkS0NlMyOzF:L3G+
MDA-MB-231 Ml;hSpVv[3Srb36gRZN{[Xl? NVrPVWVIOTBiwsXN MUmyOEBp MXrEUXNQyqB? Mmn5doVlfWOnczDQMXNVSVR|IHX4dJJme3Orb36= Mn2yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|N{[1PFYoRjJ2M{e2OVg3RC:jPh?=
SUM-159 MVvGeY5kfGmxbjDBd5NigQ>? NUCybmlLOTBiwsXN NES5PFUzPCCq M4LjXGROW00EoB?= MU\y[YR2[2W|IGCtV3RCXDNiZYjwdoV{e2mxbh?= NEHhPGQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO3OlU5Pid-MkSzO|Y2QDZ:L3G+
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MDA-MB-231 NYLpeItsSW62aXPhcoNmeiCjc4PhfS=> M37ycFEhfG9iMUCgeW0> MX:0PEBpenN? NXLKZppvSW62aXPhcoNmeiCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZZN{\XO|ZXSgZZMh[2WubDDndo94fGhiaX7obYJqfGmxbjygZZBweHSxc3nzJIFv\CClZXzseYxieiCvb4LwbI9td2erY3HsJINp[W6pZYOgZZQhOSC2bzCxNEB2VSCjZoTldkA1QCCqcoOgZpkhdGmpaISgcYlkem:|Y3;wfS=> MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyNEm2Okc,OjR7MES5OlY9N2F-
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Assay
Methods Test Index PMID
Western blot p-STAT3 / STAT3 ; Survivin / c-Myc / Bcl-xl ; PARP / C-PARP / Caspase-3 / C-Caspse-3 25261365 23382914
Immunofluorescence p-STAT3 / STAT3 / Survivin 25261365
Growth inhibition assay Cell viability 23382914
ELISA BDNF 27456333

Protocol (from reference)

Kinase Assay:

[1]

  • High-Throughput Screening and FluorescencePolarization Assays:

    Screening is performed at approximately 30 °C. The specificity ofscreening hits is validated in analogous assays for binding of thetest compounds to the SH2 domains of STAT1, STAT5, and Lck. The final concentration of buffer components used for all FP assays is 10 mM HEPES (pH 7.5), 1 mM EDTA, 0.1% Nonidet P-40, 50 mM NaCl, and 10% DMSO. The absence of dithiothreitol is essential for inhibitory activity. The sequences of the peptides are: STAT3, 5-carboxyfluorescein-GY(PO3H2)LPQTV-NH2; STAT1, 5-carboxyfluorescein-GY(PO3H2)DKPHVL; STAT5, 5-carboxyfluorescein-GY(PO3H2)LVLDKW; and Lck, 5-carboxyfluorescein-GY(PO3H2)EEIP. For specificity analysis at 30 °C, proteins are used at 150 nM (STAT1, STAT3, and STAT5). For specificity analysis at 37 °C, proteins are used at 370 nM (STAT3) or 100 nM (Lck). Proteins are incubated with test compounds in Eppendorf tubes at the indicated temperatures for 60 min prior addition of therespective 5-carboxyfluorescein labeled peptides (final concentration: 10 nM).Before measurement at room temperature, the mixtures are allowed to equilibrate for at least 30 min. Test compounds are used at the indicated concentrations diluted from a 20× stock in DMSO. Binding curves and inhibition curves are fitted with SigmaPlot. All competition curves are repeated three times in independent experiment

Cell Research:

[2]

  • Cell lines: Ly3 cells
  • Concentrations: ~2.5 μM
  • Incubation Time: 48 h
  • Method:

    MTS

Animal Research:

[2]

  • Animal Models: Ly3 parental NOD/SCID IL2R null xenograft
  • Dosages: 3.75 mg/kg every day
  • Administration: intra tumor

Solubility (25°C)

In vitro

DMSO 42 mg/mL
(198.87 mM)
Water Insoluble
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O
For best results, use promptly after mixing.

10mg/mL

Chemical Information

Molecular Weight 211.19
Formula

C8H5NO4S

CAS No. 19983-44-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC(=CC2=C1C=CS2(=O)=O)[N+](=O)[O-]

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Frequently Asked Questions

Question 1:
We need to dissolve the compound for i.p. injection, could you give me some suggestions?

Answer:
S7024 Stattic can be dissolved in 2% DMSO/PEG 300/dd H2O at 8 mg/mL as a clear solution.

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