Stattic

For research use only.

Catalog No.S7024

139 publications

Stattic Chemical Structure

CAS No. 19983-44-9

Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1. Stattic induces apoptosis.

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Selleck's Stattic has been cited by 139 publications

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Choose Selective STAT Inhibitors

Biological Activity

Description Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1. Stattic induces apoptosis.
Features Stattic is the first non-peptide small molecule with inhibitory activity against STAT3 SH2 domain regardless of the STAT3 phosphorylation state in vitro.
Targets
STAT3 [1]
(Cell-free assay)
5.1 μM
In vitro

Stattic inhibits binding of a phosphotyrosine-containing peptide derived from the gp130 receptor to the STAT3 SH2 domain in a strongly temperature-dependent manner. Stattic only has a very weak effect on binding of a tyrosinephosphorylated peptide to the SH2-domain of the tyrosine kinase Lck. And it doesn’t inhibit dimerization of two other dimeric transcription factors (c-Myc/Max and Jun/Jun). It also inhibits fluorescein-labeled phosphopeptides to the SH2 domains of STAT1 and STAT5b. Stattic selectively inhibits DNA binding of STAT3 homodimers at a concentration of 10 μM. Shown to inhibit cellular phosphorylation of STAT3 at Tyr705 with little effect towards STAT1 phosphorylation at Tyr701 (in HepG2 cells) or the phosphorylation of JAK1, JAK2, and c-Src (in MDA-MB-231 and MDA-MB-235S cells). Stattic increases the apoptotic rate of STAT3-dependent breast cancer cell lines. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H9c2 M4fSV2Z2dmO2aX;uJGF{e2G7 M1HBeFEx6oDLzszN NYfpZo12POLCiXi= M1TKV5JmfmW{c3XzJJRp\SCnZn\lZ5R{KG:oIFnMMVI4 NXy5S5hlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zN|k3OzNpPkK2N|M6PjN|PD;hQi=>
NPC  M4S3dmZ2dmO2aX;uJGF{e2G7 NX:1NWR[OC15LkWgxtVO NEfG[GRi[m:uaYPo[ZMhTU2WLXzpb4UhdW:uZXP1cIFzKGGudHXyZZRqd26|LDDhcoQh[2WubDDtbYdz[XSrb36gZY5lKGmwdnHzbY9vKGmwZIXj[YQh[nliUlvJVEBsdm:la3Tve44> NIPkbYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUmxOVQ{OCd-MkW5NVU1OzB:L3G+
HASMC M{XOTWZ2dmO2aX;uJGF{e2G7 M371e|EvOjVvNTFOwG0> M4i2WVIxKG2rbh?= NIPwUIVFVVORwrC= NFvOc41qdmirYnn0d{BxNSi\KT3TWGFVNTFuMzy1JJNq\26jbIRCpC=> NF7PXYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi0PVYzOid-MkW4OFk3OjJ:L3G+
H9c2  NEPQeFdHfW6ldHnvckBCe3OjeR?= M33VO|IwOTBizszN M2fJcFIhcA>? MVfEUXNQyqB? MVfhZpJw\2G2ZYRCpJRp\SCleYTvdJJwfGWldHn2[UBm\m[nY4TzJI9nKEmOLUK3JIFo[Wmwc4SgV2g> MoDyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6MkC5NFcoRjJ3OEKwPVA4RC:jPh?=
A431 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVqyxsDPxE1? MnLwNuKhcA>? NGLhc5FjdG:la4OgSWdHNXKndnXyd4VlKGSnY4LlZZNmeyCrbjDj[YxtKH[rYXLpcIl1gQ>? MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd{MESzOUc,OjV5MkC0N|U9N2F-
A431 MkX1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUSyxsDPxE1? M173UlLDqGh? M1PD[Ylv[3KnYYPld{BqdiCjcH;weI9{cXNiaX7keYNm\CCkeTDzbIlsd26rbh?= NHjYfVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUeyNFQ{PSd-MkW3NlA1OzV:L3G+
SiHa NUDQc5NFS2WubDDWbYFjcWyrdImgRZN{[Xl? NHrQTlA2NTd3IH7N NFXvWmwzPMLiaNMg NI[wXIt{cG:5czDtc5JxcG:ub3f5JI9nKGFidInwbYNidCCjcH;weI91cWNiY3XscEBidmRiZH;z[U1l\XCnbnTlcpQhdG:|czDv[kBk\WyuII\pZYJqdGm2edMg M13DS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUO5OlQ1Lz5{NUWzPVY1PDxxYU6=
SiHa M1:4dmZ2dmO2aX;uJGF{e2G7 MoH3OU04PSCwTR?= NIHGRpgzPMLiaNMg M1PSe5Jm\HWlZYOgeIhmyqCyaH;zdIhwenmuYYTpc44h[XRidHjlJJR6em:|aX7lJJJme2mmdXWgO|A2yqB? NXHXXXhlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1N|k3PDRpPkK1OVM6PjR2PD;hQi=>
ECA109 MlTWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1j1TVAuOjBizszN NXL5bnVSOjUEoHlCpC=> MWDJR|UxRTVwNUCg{txO MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR7MkS4NEc,OjV2OUK0PFA9N2F-
TE13 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUCwMVIxKM7:TR?= NIixS5MzPMLiaNMg MVfJR|UxRTZwMUZCpO69VQ>? NYPlO4pFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0PVI1QDBpPkK1OFkzPDhyPD;hQi=>
KYSE150  M2O5bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWXBXWpPOC1{MDFOwG0> NHzvUFczPMLiaNMg NHX0do1KSzVyPUGyMlY1yqEQvF2= M1LDOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEmyOFgxLz5{NUS5NlQ5ODxxYU6=
ECA109 MXjDcI9vd2enbnnjJHN2en[rdnHsJGF{e2G7 Mn;tNE42yqEQvF2= NXPqTlRxOjUEoHlCpC=> NIjWOGN{fXCycnXzd4V{KHSqZTDjcI9vd2enbnnjJIZwem2jdHnvci=> MnjYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2OUK0PFAoRjJ3NEmyOFgxRC:jPh?=
TE13 Ml33R4xwdm:pZX7pZ{BUfXK4aY\hcEBCe3OjeR?= MlnlNE42yqEQvF2= NHLsUGMzPMLiaNMg NHnxVFl{fXCycnXzd4V{KHSqZTDjcI9vd2enbnnjJIZwem2jdHnvci=> NHTLR5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS5NlQ5OCd-MkW0PVI1QDB:L3G+
KYSE150  MlTwR4xwdm:pZX7pZ{BUfXK4aY\hcEBCe3OjeR?= Mke3NE42yqEQvF2= NWLNSph{OjUEoHlCpC=> NYr3OGR2e3WycILld5NmeyC2aHWgZ4xwdm:pZX7pZ{Bnd3KvYYTpc44> MorBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2OUK0PFAoRjJ3NEmyOFgxRC:jPh?=
ECA109 M3LxUWZ2dmO2aX;uJGF{e2G7 MWSwMlXDqM7:TR?= NEOwWm4zPMLiaNMg MV3lcohidmOnczDJVk1qdmS3Y3XkJIdmdmW{YYTpc44hd2ZiRGPCdy=> NVni[XlDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0PVI1QDBpPkK1OFkzPDhyPD;hQi=>
PC3M-1E8 NX;vTJF1TnWwY4Tpc44hSXO|YYm= M2fWUFIvPS93L{GwJO69VQ>? MkHMNE01KGh? NHPmRY9qdmirYnn0d{B1cGViU2TBWFMh[WO2aY\heIlwdiCrbjDhJIRwe2VvIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XMEoB?= NGHIOWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK2NVM3PSd-MkWyOlE{PjV:L3G+
PC3M-1E8 M2PnZmZ2dmO2aX;uJGF{e2G7 NHnBXJYyOCEQvF2= NVzOXlU3OjRiaB?= MlXZ[I94dnKnZ4XsZZRmeyCEY3ytfGwtKHO3co\peolvKGGwZDDjMW16[w>? M{Tmb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Mk[xN|Y2Lz5{NUK2NVM3PTxxYU6=
PC3M-1E8 MlfJSpVv[3Srb36gRZN{[Xl? NWizN29HOTBizszN NXWwXINEOjRiaB?= NF3yb3lqdmirYnn0d{BKVC14IHnu[JVk\WRiU2TBWFMh[WO2aY\heIlwdiCjbnSgeIhmKEmOLU[tbY5lfWOnZDDTWGFVOyCjY4TpeoF1cW:w NWL6cGtTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyOlE{PjVpPkK1NlYyOzZ3PD;hQi=>
PC3M-1E8 M1fVXmNtd26xZ3XubYMhW3W{dnn2ZYwhSXO|YYm= M1PLV|IvPS93L{GwJO69VQ>? NV[z[mhbcW6qaXLpeJPDqHSqZTDjc4xwdnliZn;ycYF1cW:wIIPp[45q\mmlYX70cJk> MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ4MUO2OUc,OjV{NkGzOlU9N2F-
MDA-MB-231 M2PQPGZ2dmO2aX;uJGF{e2G7 Ml7wNlAh|ryP MXOyJIg> NFnVNGpmgGirYnn0d{BUdmGrbDDhcoQhTS2lYXTo[ZJqdiCneIDy[ZN{cW:w MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF3M{O0PUc,OjVzNUOzOFk9N2F-
H9c2 NEHiVlhHfW6ldHnvckBCe3OjeR?= Mke0NlAhyrWP MUOzNEBucW5? MlrmSG1UV8Li MlexZYJwdGm|aHXzJJBzd3CxZn;sMYlv\HWlZXSgRWtVKHCqb4PwbI9zgWyjdHnvckBifCCkb4ToJJNmejR5MzDhcoQhfGi{M{C4 NXX5fHd5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNFUxPjdpPkK1NVA2ODZ5PD;hQi=>
HaCaT  NXjJTXhjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVWxNEDDvU1? MWmyNEBucW5? M1fORmROW00EoB?= M2jrToVvcGGwY3XzJJNwemGoZX7pZk0h[W6mIIP1col1cW6rYj3pcoR2[2WmIHfyc5d1cCCrbnjpZol1cW:w NEW5UI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCxN|kxPyd-MkWwNVM6ODd:L3G+
Caki-1 MmX2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV7OUIhkOTBiwsXN MnnyNlAhdWmw Mo\BSG1UV8Li MoDt[Y5p[W6lZYOgd49z[W[nbnniMUBidmRic4XubZRqdmmkLXnu[JVk\WRiZ4Lve5RpKGmwaHnibZRqd25? NWDWZ45bRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwNVM6ODdpPkK1NFE{QTB5PD;hQi=>
HaCaT  NEPpZWhCeG:ydH;zbZMhSXO|YYm= NFnRTJYyOCEEtV2= NVrtXoVJOjBibXnu MoLlSG1UV8Li NGTZ[W1qdmO{ZXHz[ZMheHKxcH;yeIlwdnNib3[gZZBweHSxdHnjJINmdGy|IHT1[UB1dyC2cnXheI1mdnRid3n0bEB{d3KjZnXubYIhd3Jic4XubZRqdmmk NGP1S5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCxN|kxPyd-MkWwNVM6ODd:L3G+
FHL-primed hNSCs NFP5[pZE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NIruVmoxNjB{LUWg{txO M2fQUVczKGh? NHOwdlZt\WGmczD0c{B1cGVibH;zd{Bw\iClZXzsJJZq[WKrbHn0fUBifCCqaXfoJINwdmOnboTyZZRqd25? NYPWV5d3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OFU1OzRpPkK0PVQ2PDN2PD;hQi=>
ELL-primed hNSCs MmP3R4VtdCCYaXHibYxqfHliQYPzZZk> MWCwMlAzNTVizszN MWq3NkBp M133Voxm[WS|IITvJJRp\SCub4PzJI9nKGOnbHygeoli[mmuaYT5JIF1KGirZ3igZ49v[2WwdILheIlwdg>? Mn[yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NEW0N|QoRjJ2OUS1OFM1RC:jPh?=
SS M{HZPGNmdGxiVnnhZoltcXS7IFHzd4F6 NWfoO|BxOS1zMDFOwG0> NXfiNZJIPzJiaB?= MnrjSG1UVw>? MWLjZZV{\XNiYTDkc5NmNWSncHXu[IVvfCCrbnjpZol1cW:wIH;mJJRp\SC4aXHibYxqfHoEoB?= NFnFWIs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe1OlEyOSd-MkS3OVYyOTF:L3G+
SeAx  MVvD[YxtKF[rYXLpcIl1gSCDc4PhfS=> Mnz4NU0yOCEQvF2= NHu2eYk4OiCq M3r6dGROW09? MkjSZ4F2e2W|IHGg[I9{\S2mZYDlcoRmdnRiaX7obYJqfGmxbjDv[kB1cGVidnnhZoltcXS7wrC= MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd3NkGxNUc,OjR5NU[xNVE9N2F-
HuT-78 NIDtfmVE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NUnGZVZROS1zMDFOwG0> NF:2Rm04OiCq M4LTVGROW09? NWPneVZ7[2G3c3XzJIEh\G:|ZT3k[ZBmdmSnboSgbY5pcWKrdHnvckBw\iC2aHWgeoli[mmuaYT5xsA> MlW3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5NU[xNVEoRjJ2N{W2NVEyRC:jPh?=
CD4+ NXTE[IdmSXCxcITvd4l{KEG|c3H5 NX76ZopHOTEkgJpOwI0> NInPR2QzPCCq MmG5SG1UVw>? NGG4ZoVqdmS3Y3XzJIFxd3C2b4Ppd{B{fHKxbnfsfS=> NUXURZFTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3OVYyOTFpPkK0O|U3OTFzPD;hQi=>
MCF-7 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYCwMlQ3QS1|Lke1JO69VQ>? NXnJPYRlPSCm NWLRRmR3emWmdXPld{Bk\WyuIH71cYJmeiC|aXfubYZq[2GwdHz5 NITKd2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEeyPFA4QCd-MkS3NlgxPzh:L3G+
MCF-7/LCC1 NEizdIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4PMVlAvPDZ7LUOuO|Uh|ryP MXy1JIQ> M2fOS5Jm\HWlZYOgZ4VtdCCwdX3i[ZIhe2mpbnnmbYNidnSueR?= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd{OEC3PEc,OjR5MkiwO|g9N2F-
MCF-7/LCC9 NWe2WYZpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVfDO5J[OC52NkmtN{44PSEQvF2= M2LlW|Uh\A>? NFrJW|dz\WS3Y3XzJINmdGxiboXtZoVzKHOrZ37p[olk[W62bIm= M{PJNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{K4NFc5Lz5{NEeyPFA4QDxxYU6=
HaCaT MkK1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1zxbVExKML3TR?= M1HF[FIxKG2rbh?= MlnwSG1UV8Li NVn3OZh6\W6qYX7j[ZMh\X[ncn;sbY12ey2rbnT1Z4VlKGOnbHyg[5Jwf3SqIHnubIljcXSrb36= NILrNpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NESyN|E{OSd-MkS0NlMyOzF:L3G+
HaCaT Mm\sRZBweHSxc3nzJGF{e2G7 M4LFRlExKML3TR?= MX6yNEBucW5? M4nT[WROW00EoB?= Mmi1[Y5p[W6lZYOgeIhmKGGyb4D0c5Rq[yCnZn\lZ5R{KG:oIHX2[ZJwdGmvdYO= M2HHWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEKzNVMyLz5{NESyN|E{OTxxYU6=
MDA-MB-231 NVzZTXQ1TnWwY4Tpc44hSXO|YYm= NHjyOYwyOCEEtV2= NVvId4MyOjRiaB?= NUC5XlJxTE2VT9Mg NEi5WYxz\WS3Y3XzJHAuW1SDVEOg[ZhxemW|c3nvci=> M2rycFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{e2OVg3Lz5{NEO3OlU5PjxxYU6=
SUM-159 NVPxc3oyTnWwY4Tpc44hSXO|YYm= NXjac2k6OTBiwsXN NX3uSnhKOjRiaB?= M3vaSWROW00EoB?= NELCSZNz\WS3Y3XzJHAuW1SDVEOg[ZhxemW|c3nvci=> M3y5c|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{e2OVg3Lz5{NEO3OlU5PjxxYU6=
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A549 MnzKRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M4X1NFczKGi{cx?= MWrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF3NEmgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDRwNDFOwG0v M{CyUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5N{G4OFcxLz5{N{exPFQ4ODxxYU6=
AD293 M1;6PGZ2dmO2aX;uJIF{e2G7 MoPJOkBpenN? MY\Jcohq[mm2aX;uJI9nKEmITnfhcY1iNXO2aX31cIF1\WRiR1\QM2ZNSUdvdHHn[4VlKFOWQWSzJIRqdWW{aYrheIlwdiCrbjDoeY1idiCDREK5N{Bk\WyuczDpcoN2[mG2ZXSg[o9zKDZiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrczygTWM2OCB;IEWuNUDPxE1w NELrU2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEKyPFAxOCd-M{CyNlgxODB:L3G+
MDA-MB-231 MYLGeY5kfGmxbjDhd5NigQ>? NY\zNJF2OSC2bzCxNEB2VQ>? M1rmZ|EzKGi{cx?= M2nkZWlvcGmkaYTpc44hd2ZiU2TBWFMheGixc4Doc5J6dGG2aX;uJIF1KFS7ckewOUBqdiCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIF1KDFidH:gNVAhfU1iYX\0[ZIhOTJiaILzJIJ6KHenc4Tldo4h[myxdDDhcoFtgXOrcx?= NEftUXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwOFk3Pid-MkS5NFQ6PjZ:L3G+
MDA-MB-231 Ml3QRY51cWOjbnPldkBie3OjeR?= NXXqdIR3OSC2bzCxNEB2VQ>? NFXyUGg1QCCqcoO= NUPmcIpYSW62aXPhcoNmeiCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZZN{\XO|ZXSgZZMh[2WubDDndo94fGhiaX7obYJqfGmxbjygZZBweHSxc3nzJIFv\CClZXzseYxieiCvb4LwbI9td2erY3HsJINp[W6pZYOgZZQhOSC2bzCxNEB2VSCjZoTldkA1QCCqcoOgZpkhdGmpaISgcYlkem:|Y3;wfS=> NW\XVmhZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFQ6PjZpPkK0PVA1QTZ4PD;hQi=>
MDA-MB-231 NGDvfFZHfW6ldHnvckBie3OjeR?= MVSxJJRwKDFyIIXN MVuxNkBpenN? MXfE[YNz\WG|ZTDpckBUXEGWMzDwdo91\WmwIHX4dJJme3Orb36gbY4hcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDheEAyKHSxIEGwJJVOKGGodHXyJFEzKGi{czDifUB4\XO2ZYLuJIJtd3RiYX7hcJl{cXN? MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyNEm2Okc,OjR7MES5OlY9N2F-
MCF7 NUDi[G0yTnWwY4Tpc44h[XO|YYm= M1PWXlEzKGi{cx?= NW\tPGgyUW6qaXLpeIlwdiCxZjDTWGFVOyCyaH;zdIhwenmuYYTpc44h[XRiWUewOUBqdiCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFEzKGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7Nk[4PUc,OjZ|OU[2PFk9N2F-
MDA-MB-435S MlnWSpVv[3Srb36gZZN{[Xl? MX[xNkBpenN? MmD2TY5pcWKrdHnvckBw\iCVVFHUN{BxcG:|cHjvdplt[XSrb36gZZQhYTdyNTDpckBpfW2jbjDNSGEuVUJvNEO1V{Bk\WyuczDh[pRmeiBzMjDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz NFu2PII9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5OlY5QSd-Mk[zPVY3QDl:L3G+
MDA-MB-231 NXfJV5ZGTnWwY4Tpc44h[XO|YYm= NHXlRZoyOiCqcoO= MlTKTY5pcWKrdHnvckBw\iCVVFHUN{BxcG:|cHjvdplt[XSrb36gZZQhYTdyNTDpckBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHmeIVzKDF{IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= MmLRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OU[2PFkoRjJ4M{m2Olg6RC:jPh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-STAT3 / STAT3 ; 

PubMed: 25261365     


(A) PC3M-1E8 cells were treated with different doses of Stattic for 4 h. (B) PC3M-1E8 cells were treated with 10 μM Stattic for different time points. 

Survivin / c-Myc / Bcl-xl ; 

PubMed: 25261365     


Western blot analysis of STAT3 downstream targets in PC3M-1E8 cells treated with 10 μM Stattic for 24 h. 

PARP / C-PARP / Caspase-3 / C-Caspse-3 ; 

PubMed: 23382914     


NPC cells were exposed to the indicated concentrations of Stattic for 48 h; apoptotic cells were measured by western blot analysis of cleaved PARP and cleaved caspase-3. Protein levels were quantified using ImageJ software. 

25261365 23382914
Immunofluorescence
p-STAT3 / STAT3 / Survivin ; 

PubMed: 25261365     


Cellular localization of pSTAT3 and survivin (green) was observed by confocal microscopy after immunofluorescent staining of PC3M-1E8 cancer cells treated with 10 μM Stattic.

25261365
Growth inhibition assay
Cell viability; 

PubMed: 23382914     


Stattic inhibits the cell viability of NPC cells in a time-dependent manner. CNE2 cells were treated with 8 µM Stattic for the indicated times, and cell viability was measured by the MTT assay. 

23382914
ELISA
BDNF; 

PubMed: 27456333     


BDNF secretion was analyzed using ELISA in A549 and H1299 cells treated with or without Stattic. Results are shown as the mean ± S.E. n = 3; *p < 0.05, vs Control group, Student’s t-test. 

27456333

Protocol

Kinase Assay:

[1]

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High-Throughput Screening and FluorescencePolarization Assays:

Screening is performed at approximately 30 °C. The specificity ofscreening hits is validated in analogous assays for binding of thetest compounds to the SH2 domains of STAT1, STAT5, and Lck. The final concentration of buffer components used for all FP assays is 10 mM HEPES (pH 7.5), 1 mM EDTA, 0.1% Nonidet P-40, 50 mM NaCl, and 10% DMSO. The absence of dithiothreitol is essential for inhibitory activity. The sequences of the peptides are: STAT3, 5-carboxyfluorescein-GY(PO3H2)LPQTV-NH2; STAT1, 5-carboxyfluorescein-GY(PO3H2)DKPHVL; STAT5, 5-carboxyfluorescein-GY(PO3H2)LVLDKW; and Lck, 5-carboxyfluorescein-GY(PO3H2)EEIP. For specificity analysis at 30 °C, proteins are used at 150 nM (STAT1, STAT3, and STAT5). For specificity analysis at 37 °C, proteins are used at 370 nM (STAT3) or 100 nM (Lck). Proteins are incubated with test compounds in Eppendorf tubes at the indicated temperatures for 60 min prior addition of therespective 5-carboxyfluorescein labeled peptides (final concentration: 10 nM).Before measurement at room temperature, the mixtures are allowed to equilibrate for at least 30 min. Test compounds are used at the indicated concentrations diluted from a 20× stock in DMSO. Binding curves and inhibition curves are fitted with SigmaPlot. All competition curves are repeated three times in independent experiment
Cell Research:

[2]

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  • Cell lines: Ly3 cells
  • Concentrations: ~2.5 μM
  • Incubation Time: 48 h
  • Method:

    MTS


    (Only for Reference)
Animal Research:

[2]

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  • Animal Models: Ly3 parental NOD/SCID IL2R null xenograft
  • Dosages: 3.75 mg/kg every day
  • Administration: intra tumor
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 42 mg/mL (198.87 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O
For best results, use promptly after mixing.
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 211.19
Formula

C8H5NO4S

CAS No. 19983-44-9
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC(=CC2=C1C=CS2(=O)=O)[N+](=O)[O-]

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    We need to dissolve the compound for i.p. injection, could you give me some suggestions?

  • Answer:

    S7024 Stattic can be dissolved in 2% DMSO/PEG 300/dd H2O at 8 mg/mL as a clear solution.

STAT Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID