Stattic

Catalog No.S7024

Stattic Chemical Structure

Molecular Weight(MW): 211.19

Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1.

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In DMSO USD 90 In stock
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Cited by 66 Publications

Purity & Quality Control

Choose Selective STAT Inhibitors

Biological Activity

Description Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1.
Features Stattic is the first non-peptide small molecule with inhibitory activity against STAT3 SH2 domain regardless of the STAT3 phosphorylation state in vitro.
Targets
STAT3 [1]
(Cell-free assay)
5.1 μM
In vitro

Stattic inhibits binding of a phosphotyrosine-containing peptide derived from the gp130 receptor to the STAT3 SH2 domain in a strongly temperature-dependent manner. Stattic only has a very weak effect on binding of a tyrosinephosphorylated peptide to the SH2-domain of the tyrosine kinase Lck. And it doesn’t inhibit dimerization of two other dimeric transcription factors (c-Myc/Max and Jun/Jun). It also inhibits fluorescein-labeled phosphopeptides to the SH2 domains of STAT1 and STAT5b. Stattic selectively inhibits DNA binding of STAT3 homodimers at a concentration of 10 μM. Shown to inhibit cellular phosphorylation of STAT3 at Tyr705 with little effect towards STAT1 phosphorylation at Tyr701 (in HepG2 cells) or the phosphorylation of JAK1, JAK2, and c-Src (in MDA-MB-231 and MDA-MB-235S cells). Stattic increases the apoptotic rate of STAT3-dependent breast cancer cell lines. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H9c2 NIPNfoxHfW6ldHnvckBCe3OjeR?= NYXpWoIyOTEkgJpOwG0> MUG05qCKcA>? MlnmdoV3\XK|ZYOgeIhmKGWoZnXjeJMhd2ZiSVytNlc> MXmyOlM{QTZ|Mx?=
NPC  MnfVSpVv[3Srb36gRZN{[Xl? NEPnU3IxNTdwNTFCuW0> NXPwXpZv[WKxbHnzbIV{KEWPVD3sbYtmKG2xbHXjeYxieiCjbITldoF1cW:wczygZY5lKGOnbHygcYloemG2aX;uJIFv\CCrbo\hd4lwdiCrbnT1Z4VlKGK7IGLLTXAhc26xY3vkc5dv NWDnPZFYOjV7MUW0N|A>
HASMC M4HaRmZ2dmO2aX;uJGF{e2G7 NGS2cG8yNjJ3LUWg{txO M1e4PFIxKG2rbh?= MVTEUXNQyqB? MUDpcohq[mm2czDwMUh[MS2VVFHUMVEtOyx3IIPp[45idHQEoB?= NXXFeFB7OjV6NEm2NlI>
H9c2  MV\GeY5kfGmxbjDBd5NigQ>? NEK1WmozNzFyIN88US=> MoTjNkBp NVLjUmpLTE2VT9Mg MmLzZYJzd2ejdHXzxsB1cGViY4n0c5Bzd3SnY4TpeoUh\W[oZXP0d{Bw\iCLTD2yO{Bi\2GrboP0JHNJ NH3qe2wzPTh{MEmwOy=>
A431 Mn3BS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2PzdVLDqM7:TR?= NFHGfVIzyqCq NW\iPWlT[myxY3vzJGVITi2{ZY\ldpNm\CCmZXPy[YF{\XNiaX6gZ4VtdCC4aXHibYxqfHl? MViyOVczODR|NR?=
A431 MlziS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1jpVlLDqM7:TR?= NGDNUYYzyqCq MlzpbY5kemWjc3XzJIlvKGGyb4D0c5NqeyCrbnT1Z4VlKGK7IIPobYtwdmmw MmnRNlU4OjB2M{W=
SiHa M4\wNWNmdGxiVnnhZoltcXS7IFHzd4F6 M2jibFUuPzVibl2= M1HyTVI1yqCqwrC= NH7JfYt{cG:5czDtc5JxcG:ub3f5JI9nKGFidInwbYNidCCjcH;weI91cWNiY3XscEBidmRiZH;z[U1l\XCnbnTlcpQhdG:|czDv[kBk\WyuII\pZYJqdGm2edMg NYjafpd4OjV3M{m2OFQ>
SiHa NV;zPHlWTnWwY4Tpc44hSXO|YYm= MWG1MVc2KG6P NInGRYQzPMLiaNMg MVHy[YR2[2W|IITo[eKheGixc4Doc5J6dGG2aX;uJIF1KHSqZTD0fZJwe2mwZTDy[ZNq\HWnIEewOeKh M1rSe|I2PTN7NkS0
ECA109 NVfIWXY5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEGxU3UxNTJyIN88US=> NX3wVFE{OjUEoHlCpC=> M3\C[WlEPTB;NT61NEDPxE1? M2Txb|I2PDl{NEiw
TE13 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVXoSHh7OC1{MDFOwG0> NF;vWZUzPMLiaNMg MYLJR|UxRTZwMUZCpO69VQ>? M1GwV|I2PDl{NEiw
KYSE150  NVzQW25RT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVewMVIxKM7:TR?= M3rFTFI1yqCqwrC= NHqyU5NKSzVyPUGyMlY1yqEQvF2= M4PCTFI2PDl{NEiw
ECA109 M1\qXmNtd26xZ3XubYMhW3W{dnn2ZYwhSXO|YYm= NILvVGQxNjYEoN88US=> Mmr3NlTDqGkEoB?= Ml;zd5VxeHKnc4Pld{B1cGViY3zvco9o\W6rYzDmc5Ju[XSrb36= MoixNlU1QTJ2OEC=
TE13 MXfDcI9vd2enbnnjJHN2en[rdnHsJGF{e2G7 NWfpfpA1OC53wrFOwG0> M3LhblI1yqCqwrC= NVmybWJJe3WycILld5NmeyC2aHWgZ4xwdm:pZX7pZ{Bnd3KvYYTpc44> NIHjUlAzPTR7MkS4NC=>
KYSE150  M{nFSmNtd26xZ3XubYMhW3W{dnn2ZYwhSXO|YYm= NUjoc45sOC53wrFOwG0> NX:2cnY3OjUEoHlCpC=> NHfGc5l{fXCycnXzd4V{KHSqZTDjcI9vd2enbnnjJIZwem2jdHnvci=> M4fsZlI2PDl{NEiw
ECA109 M{W5TWZ2dmO2aX;uJGF{e2G7 NXu4WJIzOC53wrFOwG0> MkO5NlTDqGkEoB?= Ml\6[Y5p[W6lZYOgTXIucW6mdXPl[EBo\W6ncnH0bY9vKG:oIFTTRpM> MYCyOVQ6OjR6MB?=
PC3M-1E8 MXrGeY5kfGmxbjDBd5NigQ>? MknWNk42NzVxMUCg{txO NWPBVnRUOC12IHi= MnjkbY5pcWKrdIOgeIhmKFOWQWSzJIFkfGm4YYTpc44hcW5iYTDkc5NmNSCjbnSgeIlu\S2mZYDlcoRmdnRibXHucoVzyqB? M4fJVFI2OjZzM{[1
PC3M-1E8 M3XyRWZ2dmO2aX;uJGF{e2G7 NIPBdnUyOCEQvF2= NFK5O24zPCCq MoC2[I94dnKnZ4XsZZRmeyCEY3ytfGwtKHO3co\peolvKGGwZDDjMW16[w>? M4DPXVI2OjZzM{[1
PC3M-1E8 M1fDeWZ2dmO2aX;uJGF{e2G7 NFS2VWgyOCEQvF2= NWXVNIkxOjRiaB?= MVLpcohq[mm2czDJUE03KGmwZIXj[YQhW1SDVEOgZYN1cX[jdHnvckBidmRidHjlJGlNNTZvaX7keYNm\CCVVFHUN{Bi[3SrdnH0bY9v NEHzXmgzPTJ4MUO2OS=>
PC3M-1E8 MX7DcI9vd2enbnnjJHN2en[rdnHsJGF{e2G7 NXLqR5hwOi53L{WvNVAh|ryP NFL4c3FqdmirYnn0d:KhfGinIHPvcI9vgSCob4LtZZRqd25ic3nncolncWOjboTsfS=> MnTyNlUzPjF|NkW=
MDA-MB-231 NH3pb2NHfW6ldHnvckBCe3OjeR?= MUiyNEDPxE1? NUfFbJYzOiCq NVfXfpZL\XiqaXLpeJMhW26jaXygZY5lKEVvY3HkbIVzcW5iZYjwdoV{e2mxbh?= NF;SeFUzPTF3M{O0PS=>
H9c2 NHzhWG5HfW6ldHnvckBCe3OjeR?= NULmcId4OjBiwsXN Mom1N|AhdWmw MYPEUXNQyqB? NFLhVnBi[m:uaYPo[ZMheHKxcH;mc4wucW6mdXPl[EBCU1RicHjvd5Bpd3K7bHH0bY9vKGG2IHLveIghe2W{NEezJIFv\CC2aIKzNFg> M2O2WVI2OTB3ME[3
HaCaT  MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVSxNEDDvU1? M1XZ[lIxKG2rbh?= NYfEeHR4TE2VT9Mg NH;ib|VmdmijbnPld{B{d3KjZnXubYIuKGGwZDDzeY5qfGmwaXKtbY5lfWOnZDDndo94fGhiaX7obYJqfGmxbh?= NF;rbFEzPTBzM{mwOy=>
Caki-1 M1fiPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYTafGdxOTBiwsXN NFrWenQzOCCvaX6= NUfuTGF6TE2VT9Mg Ml;o[Y5p[W6lZYOgd49z[W[nbnniMUBidmRic4XubZRqdmmkLXnu[JVk\WRiZ4Lve5RpKGmwaHnibZRqd25? NF7ncWUzPTBzM{mwOy=>
HaCaT  NE\BVXFCeG:ydH;zbZMhSXO|YYm= MVixNEDDvU1? NGnoOVUzOCCvaX6= NUexb5doTE2VT9Mg NHzN[mJqdmO{ZXHz[ZMheHKxcH;yeIlwdnNib3[gZZBweHSxdHnjJINmdGy|IHT1[UB1dyC2cnXheI1mdnRid3n0bEB{d3KjZnXubYIhd3Jic4XubZRqdmmk NIHSRlkzPTBzM{mwOy=>
FHL-primed hNSCs MX7D[YxtKF[rYXLpcIl1gSCDc4PhfS=> MmnWNE4xOi13IN88US=> MU[3NkBp MWLs[YFleyC2bzD0bIUhdG:|czDv[kBk\WyuII\pZYJqdGm2eTDheEBpcWeqIHPvcoNmdnS{YYTpc44> M2PFOVI1QTR3NEO0
ELL-primed hNSCs MWXD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M1zDTVAvODJvNTFOwG0> M1jhVVczKGh? MmPHcIVi\HNidH:geIhmKGyxc4Ogc4Yh[2WubDD2bYFjcWyrdImgZZQhcGmpaDDjc45k\W62cnH0bY9v MorvNlQ6PDV2M{S=
SS MmCwR4VtdCCYaXHibYxqfHliQYPzZZk> NInOTI8yNTFyIN88US=> NH3helQ4OiCq M2XwTmROW09? MmjqZ4F2e2W|IHGg[I9{\S2mZYDlcoRmdnRiaX7obYJqfGmxbjDv[kB1cGVidnnhZoltcXS7wrC= M3PxbFI1PzV4MUGx
SeAx  NG\wWXpE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MkHWNU0yOCEQvF2= NHHFVnU4OiCq M2\4SmROW09? Mo\ZZ4F2e2W|IHGg[I9{\S2mZYDlcoRmdnRiaX7obYJqfGmxbjDv[kB1cGVidnnhZoltcXS7wrC= M4C2NFI1PzV4MUGx
HuT-78 M1TtVGNmdGxiVnnhZoltcXS7IFHzd4F6 MlXuNU0yOCEQvF2= NYLu[Y1oPzJiaB?= NF[3NYRFVVOR M{LJV4NifXOnczDhJIRwe2VvZHXw[Y5l\W62IHnubIljcXSrb36gc4YhfGinII\pZYJqdGm2edMg NWfqcWlZOjR5NU[xNVE>
CD4+ M4L5WGFxd3C2b4Ppd{BCe3OjeR?= MlPoNVDjiIoQvH2= MmHJNlQhcA>? MV\EUXNQ NGPVfYlqdmS3Y3XzJIFxd3C2b4Ppd{B{fHKxbnfsfS=> MlvQNlQ4PTZzMUG=
MCF-7 M1\UTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHr2W5gxNjR4OT2zMlc2KM7:TR?= NWW5VYRCPSCm NUfCUm9zemWmdXPld{Bk\WyuIH71cYJmeiC|aXfubYZq[2GwdHz5 MV:yOFczQDB5OB?=
MCF-7/LCC1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnrYNE41PjlvMz63OUDPxE1? M3jacFUh\A>? M1j5Z5Jm\HWlZYOgZ4VtdCCwdX3i[ZIhe2mpbnnmbYNidnSueR?= NFHyWIQzPDd{OEC3PC=>
MCF-7/LCC9 MkDpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYC3[Y1UOC52NkmtN{44PSEQvF2= NIq2d4Q2KGR? NEXCboxz\WS3Y3XzJINmdGxiboXtZoVzKHOrZ37p[olk[W62bIm= NVnSUos6OjR5MkiwO|g>
HaCaT Mmq5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV2xNEDDvU1? NGrESFMzOCCvaX6= M2\ze2ROW00EoB?= NWmy[m96\W6qYX7j[ZMh\X[ncn;sbY12ey2rbnT1Z4VlKGOnbHyg[5Jwf3SqIHnubIljcXSrb36= MYqyOFQzOzF|MR?=
HaCaT M4Tkc2Fxd3C2b4Ppd{BCe3OjeR?= M3PnWlExKML3TR?= MX[yNEBucW5? NXLQXWtwTE2VT9Mg MX;lcohidmOnczD0bIUh[XCxcITveIlkKGWoZnXjeJMhd2ZiZY\ldo9tcW23cx?= NIPWZ2EzPDR{M{GzNS=>
MDA-MB-231 NIDs[5VHfW6ldHnvckBCe3OjeR?= NU[zXYx6OTBiwsXN NHGy[|AzPCCq MW\EUXNQyqB? NVXNVm1SemWmdXPld{BRNVOWQWSzJIV5eHKnc4Ppc44> MnrpNlQ{PzZ3OE[=
SUM-159 NVfreIJLTnWwY4Tpc44hSXO|YYm= M{m3UFExKML3TR?= MlrZNlQhcA>? MlTVSG1UV8Li M3XDZ5Jm\HWlZYOgVE1UXEGWMzDlfJBz\XO|aX;u NWn3UHFFOjR|N{[1PFY>
SK-BR-3 M1jGNmZ2dmO2aX;uJGF{e2G7 MUCxNEDDvU1? M174RlI1KGh? M{nOPWROW00EoB?= M3fwRZJm\HWlZYOgVE1UXEGWMzDlfJBz\XO|aX;u M3XnbVI1Ozd4NUi2
MCF7-HER2 NVLlWHZ5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX;iRotxOC1zMDFOwG0> NFWzd|k1QCCq M17HOmROW09? NGjtdZFqdmS3Y3XzJINmdGxiZHXheIgh\G:|ZTDk[ZBmdmSnboTsfS=> NHf1PIQzPDJ7N{WwPC=>
MCF7-HER2 MWnGeY5kfGmxbjDBd5NigQ>? NUHmUphlPSEQvF2= NGjWW5gzPCCq M{fhcmROW09? NHXmVWRlcW2rbnnzbIV{KFOxeD2yMEBQ[3RvNDygZY5lKHOudXeg[ZhxemW|c3nvci=> Mlj6NlQzQTd3MEi=
MCF7-HER2 NFq2cZVHfW6ldHnvckBCe3OjeR?= MnqyOUDPxE1? NGXqU5gzPCCq M{PnfmROW09? Mnn0[IVkemWjc3XzJJRp\SCneIDy[ZN{cW:wIHzleoVteyCxZjDFUXQhdWG{a3Xyd{whfmmvZX70bY4h[W6mIIPseYc> MX[yOFI6PzVyOB?=
MCF7-HER2 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHuxOlc2KM7:TR?= NEXQWXMzPCCq M{DBSmROW09? MWrlcohidmOnczDj[YxtKGe{b4f0bEBqdmirYnn0bY9vKGOxbXLpcoVlKHerdHigTIVz[2WydHnu MmOyNlQzQTd3MEi=
HMECs  M{TETGZ2dmO2aX;uJGF{e2G7 M1fN[lExKM7:TR?= NWnQTIlKOiCq MVnpcohq[mm2czDJSm7PucLibXXkbYF1\WRicHjvd5Bpd3K7bHH0bY9vKG:oIGPURXQyNCCVVFHUNkBidmRiU2TBWFM> NF;xWoozPDJzMUOyOy=>
HTR8/SVneo NXz3OHVCTnWwY4Tpc44hSXO|YYm= Mlv0NeKh|ryPwrC= MYexxsBp M2DEW5N2eHC{ZYPz[YQhV1OPLXnu[JVk\WRiU2TBWFMheGixc4Doc5J6dGG2aX;u NIH5eIMzPDB4MEK0NS=>
HTR8/SVneo M2fpNGZ2dmO2aX;uJGF{e2G7 NIr1blExNjVxMdMg{txOyqB? MWe0PEBp MXLy[ZN1d3KnczD0bIUh\XiycnXzd4lwdiCxZjDFMYNi\GincnnuJJN2eHC{ZYPz[YQh[nliT2PN M1PiRlI1ODZyMkSx
HTR8/SVneo MmXjSpVv[3Srb36gRZN{[Xl? NH7tbm4yyqEQvF5CpC=> NInGXFg1QCCq MoPDd4lodmmoaXPhcpRtgSCrbnPy[YF{\XNibXnndoF1cW:wIHL5JG9UVcLi M3ixW|I1ODZyMkSx
C13* NFPrdZhCeG:ydH;zbZMhSXO|YYm= M3G4elAuOTBizszN M{XQcFI1NzR6IHi= MYHpcoR2[2W|IHHwc5B1d3OrczDpckBiKGSxc3WgZY5lKHSrbXWg[IVx\W6mZX70JI1idm6nch?= NH7PSlEzOzl4MkW1PC=>
OV2008 MlW1RZBweHSxc3nzJGF{e2G7 MorlNE0yOCEQvF2= NF7kRokzPC92ODDo MWjpcoR2[2W|IHHwc5B1d3OrczDpckBiKGSxc3WgZY5lKHSrbXWg[IVx\W6mZX70JI1idm6nch?= NUS1bHdKOjN7NkK1OVg>
C13* M3THXGFxd3C2b4Ppd{BCe3OjeR?= MkjBNlQwPDhiaB?= Mo\Q[Y5p[W6lZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZZBweHSxc3nz Mn;aNlM6PjJ3NUi=
OV2008 MUTBdI9xfG:|aYOgRZN{[Xl? MYiyOE81QCCq MYrlcohidmOnczDjbZNxdGG2aX6tbY5lfWOnZDDhdI9xfG:|aYO= M{PZT|I{QTZ{NUW4
W480  MknRSpVv[3Srb36gRZN{[Xl? MXeyMlUwOTBizszN MYqzNEBucW5? MmDFSG1UVw>? NWHlZ2NFe2Wwc3n0bZpmeyClZXzsd{B1dyClaHXtc5Ji\GmxdHjldoFxgSCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? NEjLOWozOzl|NEm3Ni=>
SW837 MlX6SpVv[3Srb36gRZN{[Xl? NXLCbWFpOi53L{GwJO69VQ>? MV6zNEBucW5? NYXJfJJpTE2VTx?= MUTz[Y5{cXSrenXzJINmdGy|IITvJINp\W2xcnHkbY91cGW{YYD5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MUKyN|k{PDl5Mh?=
T24 MkfXSpVv[3Srb36gRZN{[Xl? NUP6eYpZOi9zMD:yNEDPxE1? MnHjNlQhcA>? M4LtZ4NifXOnczDkc5NmNWSncHXu[IVvfCCrbnjpZol1cW:wIH;mJJRp\SCFWFPMNVIucW6mdXPl[EBqdmO{ZXHz[UBw\iCrbo\h[Ilv\yClZXzsdy=> NIDJcIozOzV{NkC3PS=>
CNE1 MXrGeY5kfGmxbjDBd5NigQ>? MUGyNEDDvU1? MljROFghcA>? NX3nO4E5[myxY3vzJJRp\SCLTD22JIlv[3KnYYPl[EBxcG:|cHjvdplt[XSrb36gc4YhW3SjdEO= Mki3NlM{QDJ7MUS=
CNE2 MmrKSpVv[3Srb36gRZN{[Xl? MUOyNEDDvU1? M4\vcFQ5KGh? NF\TV41jdG:la4OgeIhmKEmOLU[gbY5kemWjc3XkJJBpd3OyaH;yfYxifGmxbjDv[kBUfGG2Mx?= NXnybJdrOjN|OEK5NVQ>
HONE1 NHnWcHRHfW6ldHnvckBCe3OjeR?= NFHsfIkzOCEEtV2= NFzIWYg1QCCq M3n4RYJtd2OtczD0bIUhUUxvNjDpcoNz\WG|ZXSgdIhwe3Cqb4L5cIF1cW:wIH;mJHN1[XR| M4W3XFI{Ozh{OUG0
CNE1 M1jBbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmfDOEDPxE1? MVHzbYdvcW[rY3HueIx6KHKnZIXj[ZMh[2WubDD2bYFjcWyrdIm= NHe0N28zOzN6MkmxOC=>
CNE1 MV7GeY5kfGmxbjDBd5NigQ>? NH;LOoExNTJyIN88US=> Mo\DNE01KGh? NXG5blh1cW6qaXLpeJMhW3SjdEOgZYN1cX[jdHnvckBqdiCjIHTvd4UuKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> M1LvOFI{Ozh{OUG0
CNE2 MVXGeY5kfGmxbjDBd5NigQ>? MXWwMVIxKM7:TR?= MVuwMVQhcA>? M1zRXolvcGmkaYTzJHN1[XR|IHHjeIl3[XSrb36gbY4h[SCmb4PlMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy NFLxb5YzOzN6MkmxOC=>
HONE1 NEDReYpHfW6ldHnvckBCe3OjeR?= MVWwMVIxKM7:TR?= NX;4VGVXOC12IHi= NWPBNoxvcW6qaXLpeJMhW3SjdEOgZYN1cX[jdHnvckBqdiCjIHTvd4UuKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> NVnqbIpJOjN|OEK5NVQ>
CNE1 NELyTpBE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MmLPNE42NTZ2IN88US=> Mni3OFghcA>? NImxZZp{fXCycnXzd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> NHvqS5QzOzN6MkmxOC=>
CNE2 M2jO[mNmdGxiVnnhZoltcXS7IFHzd4F6 NVLNT5poOC53LU[0JO69VQ>? M1vPdVQ5KGh? M3rhWJN2eHC{ZYPz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy M1jlV|I{Ozh{OUG0
HONE1 M{D4dGNmdGxiVnnhZoltcXS7IFHzd4F6 NV\hN4E4OC53LU[0JO69VQ>? MWW0PEBp NH64cpZ{fXCycnXzd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> NH21c5AzOzN6MkmxOC=>
C666-1  NULnblFzS2WubDDWbYFjcWyrdImgRZN{[Xl? MUKwMlUuPjRizszN MmSwOFghcA>? NEnr[GV{fXCycnXzd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> M{LQSlI{Ozh{OUG0
CNE1 NH;EU4ZCeG:ydH;zbZMhSXO|YYm= M2rxUlExKML3TdMg M{\SRlQ5KGh? MnrObY5lfWOnczDhdI9xfG:|aYRCpC=> NIm0eI4zOzN6MkmxOC=>
CNE2 M1LUd2Fxd3C2b4Ppd{BCe3OjeR?= MYexNEDDvU4EoB?= M2PiclQ5KGh? NXrGSoFXcW6mdXPld{BieG:ydH;zbZPDqA>? NX3XeGN3OjN|OEK5NVQ>
HONE1 NFLsZ2JCeG:ydH;zbZMhSXO|YYm= NH2yXXIyOCEEtV5CpC=> NYPx[IpzPDhiaB?= NGPSc|RqdmS3Y3XzJIFxd3C2b4Ppd:Kh NYXqV4x4OjN|OEK5NVQ>
CNE2 NWr0W3pLS2WubDDWbYFjcWyrdImgRZN{[Xl? MUOxM|Ih|ryP M2nJdlQ5KGh? MoDNd4Vve2m2aYrlJINmdGy|IITvJJJi\GmxdHjldoFxgQ>? MlHHNlM{QDJ7MUS=
HONE1 NFfwcGNE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MmTsNU8zKM7:TR?= M3jBVVQ5KGh? Mn:2d4Vve2m2aYrlJINmdGy|IITvJJJi\GmxdHjldoFxgQ>? NWmwNVBUOjN|OEK5NVQ>
C666-1  MXnD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NGf5[nMyNzJizszN NHvuWIY1QCCq MYfz[Y5{cXSrenWgZ4VtdHNidH:gdoFlcW:2aHXyZZB6 NEP1VoQzOzN6MkmxOC=>
HEC-1A MnrYSpVv[3Srb36gRZN{[Xl? NGrM[ZgyyqEQvF5CpC=> MXqyOEBp NITJb2hFVVOR M3nUc4Jtd2OtczD0bIUhVVWFMkCt[Y5p[W6lZXSgbY53[XOrb36geJJq\2encnXkJIJ6KDFyJTDGRnM> NF3Be4YzOzJ4MkKwPC=>
RL95-2 MnvnSpVv[3Srb36gRZN{[Xl? NGLvNooyyqEQvF5CpC=> NEHaU5UzPCCq M4nIcmROW09? M4LWbYJtd2OtczD0bIUhVVWFMkCt[Y5p[W6lZXSgbY53[XOrb36geJJq\2encnXkJIJ6KDFyJTDGRnM> NUjrUGNDOjN{NkKyNFg>
HEC-1A NXPTU4ZOTnWwY4Tpc44hSXO|YYm= NWnkXmpnOcLizszNxsA> NUOzbXlXOjRiaB?= NXvYcJEzTE2VTx?= NYfieIZj[myxY3vzJJRp\SCPVVOyNE1mdmijbnPl[EBqdn[jc3nvckB1emmpZ3Xy[YQh[nliRVfGxsA> NUnQ[HIyOjN{NkKyNFg>
RL95-2 NEPmVXlHfW6ldHnvckBCe3OjeR?= M3LmOVHDqM7:TdMg NGnkcpozPCCq M2jndWROW09? MWTicI9kc3NidHjlJG1WSzJyLXXubIFv[2WmIHnueoF{cW:wIITybYdo\XKnZDDifUBGT0cEoB?= MU[yN|I3OjJyOB?=
CT26 MkjCSpVv[3Srb36gRZN{[Xl? MkHTNlAhdU1? MVmxJIg> NH2yTFB{fXCycnXzd4V{KEiJRj3pcoR2[2WmIG\FS2Yh\XiycnXzd4lwdsLi MoPmNlMzOzNzNkO=
UM-SCC-17B MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4rXZmlEPTB;Mj61OlIhyrFiMD60NFkh|ryPLDDHTVUxRTFwMke5JOKyKDBwMUm0JO69VQ>? MXGyNlc4ODh7OR?=
OSC-19 Mnu4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUnxOFdMUUN3ME2zMlQ5OSEEsTCwMlk2OyEQvF2sJGdKPTB;MT6zOlYhyrFiMD63O|Ah|ryP MX2yNlc4ODh7OR?=
Cal33 NXvEbnl3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXfqZVUxUUN3ME2yMlI5OiEEsTCwMlQzOyEQvF2sJGdKPTB;MT6zOFkhyrFiMD6zOlMh|ryP MXuyNlc4ODh7OR?=
UM-SCC-22B MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYLrfYNoUUN3ME2yMlY1QCEEsTCwMlU1OiEQvF2sJGdKPTB;MT6zNlAhyrFiMD6yNFQh|ryP NVmwPZFwOjJ5N{C4PVk>
UM-SCC-17B NIS1OY5HfW6ldHnvckBCe3OjeR?= MnHkNE0{OCEQvF2= NGe3T2kxNTJ2IHi= MWjpcohq[mm2czDTWGFVOyCjY4TpeoF1cW:wIHTvd4Uh[W6mIITpcYUh\GWyZX7k[Y51dHl? MXSyNlc4ODh7OR?=
OSC-19 NFv2V|FHfW6ldHnvckBCe3OjeR?= NITxb5MxNTNyIN88US=> NHPSe2oxNTJ2IHi= M1\ndolvcGmkaYTzJHNVSVR|IHHjeIl3[XSrb36g[I9{\SCjbnSgeIlu\SCmZYDlcoRmdnSueR?= NVXG[VdPOjJ5N{C4PVk>
Cal33 MWXGeY5kfGmxbjDBd5NigQ>? MYGwMVMxKM7:TR?= NWjiPI1lOC1{NDDo NXvq[VFIcW6qaXLpeJMhW1SDVEOgZYN1cX[jdHnvckBld3OnIHHu[EB1cW2nIHTldIVv\GWwdHz5 NVLHSFFHOjJ5N{C4PVk>
UM-SCC-22B NVXSPI53TnWwY4Tpc44hSXO|YYm= MYSwMVMxKM7:TR?= NV;6ZlRvOC1{NDDo MXfpcohq[mm2czDTWGFVOyCjY4TpeoF1cW:wIHTvd4Uh[W6mIITpcYUh\GWyZX7k[Y51dHl? M2DGW|IzPzdyOEm5
U-87MG M2XnNGNmdGxiVnnhZoltcXS7IFHzd4F6 MXSwMVExKM7:TR?= M1r6UVczKGh? MWXEUXNQ NH;KNG9qdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 MojLNlU1OzZ4OEK=
U-373MG Mm\FR4VtdCCYaXHibYxqfHliQYPzZZk> MlPONE0yOCEQvF2= NFH2cGs4OiCq Ml;3SG1UVw>? M3q1OYlvcGmkaYTzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? MkTONlU1OzZ4OEK=
SH-SY5Y NHTS[4xE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M1q0UlAuOTBizszN NXWyZphCPzJiaB?= NWTGcYpRTE2VTx?= M1TVRolvcGmkaYTzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? M17NV|I2PDN4Nkiy
Tu-9648 NUXhe442S2WubDDWbYFjcWyrdImgRZN{[Xl? M{\xOVAuOTBizszN MlHIO|IhcA>? NV[zbVFQTE2VTx?= NHPKcYZqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 MUeyOVQ{PjZ6Mh?=
Neuro-2a M17xSmNmdGxiVnnhZoltcXS7IFHzd4F6 MYqwMVExKM7:TR?= MVO3NkBp MmjsSG1UVw>? M{ji[4lvcGmkaYTzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? NXXYNlY5OjV2M{[2PFI>
PCNs NG\McI9E\WyuIG\pZYJqdGm2eTDBd5NigQ>? NETqdIMxNTFyIN88US=> Mnm5O|IhcA>? MmDBSG1UVw>? M2XlVIlvcGmkaYTzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? NV7SVXZnOjV2M{[2PFI>
PGCs MVHD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M{XyflAuOTBizszN Ml\IO|IhcA>? NUPDcXNuTE2VTx?= NH7QPWVqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 MlTENlU1OzZ4OEK=
RAW264.7 NWfYRlNNTnWwY4Tpc44hSXO|YYm= NH3nN|UyOCEQvF2= NGf2eWcyOiCq M1q0OYFjem:pYYTld{B1cGVibWLORUBmgHC{ZYPzbY9veyCxZjDKRWszNCCVVFHUNUwhW1SDVEKsJIFv\CCVVFHUN{BqdmS3Y3XkJIJ6KESRTjDhcoQhXC1{IITvfIlv NULsdVd[OjJ2NUS0N|E>
RAW264.7 NU\PPG0{SXCxcITvd4l{KEG|c3H5 NUTCSYNzPS9zMDFOwG0> MVq0OUBucW5? NGjDSGdmdmijbnPld{B1d3irboOgbY5lfWOnZDDhdI9xfG:|aYOgZY5lKE2PUDDsc5N{ MWiyNlQ2PDR|MR?=
SW480 NHzsfJdE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MYC1M|ExNzJyIN88US=> NXT2dml4PzJiaB?= M2T2c4lvcGmkaYTzJINmdGxidnnhZoltcXS7IH;mJJRp\SCDTFTIL{9ETDF|MzxCpINmdGy| M{\ze|IyQTByM{m3
HCT116 M{flVWNmdGxiVnnhZoltcXS7IFHzd4F6 Mo\sOU8yOC9{MDFOwG0> M33KOFczKGh? MnXqbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHlib3[geIhmKEGORFirM2NFOTN|K9MgZ4VtdHN? MW[yNVkxODN7Nx?=
DLD-1  MVLD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MXO1M|ExNzJyIN88US=> NVThWIZlPzJiaB?= MWfpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCxZjD0bIUhSUyGSDuvR2QyOzNtwrDj[Yxtew>? MUiyNVkxODN7Nx?=
SNU387  M2\3UGNmdGxiVnnhZoltcXS7IFHzd4F6 NEPWRWYzOMLizszNxsA> NXLZcotLOjRiaB?= MUTy[YR2[2W|IHPlcIwhfmmjYnnsbZR6 M1u0c|IyOzFzOUe1
SNU398 NXT4U2hjS2WubDDWbYFjcWyrdImgRZN{[Xl? NV\BO4REOjEEoN88UeKh NV\WeGhjOjRiaB?= MlXkdoVlfWOnczDj[YxtKH[rYXLpcIl1gQ>? NF7CSWUzOTNzMUm3OS=>
HepG2 M2DpR2NmdGxiVnnhZoltcXS7IFHzd4F6 MmPZNlDDqM7:TdMg MYKyOEBp NIPq[Ylz\WS3Y3XzJINmdGxidnnhZoltcXS7 NGXpfWszOTNzMUm3OS=>
Huh-7 MVvD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M1:1SlIxyqEQvF5CpC=> M13TUlI1KGh? MkPVdoVlfWOnczDj[YxtKH[rYXLpcIl1gQ>? M3PO[VIyOzFzOUe1
VSMC M2TWW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2PtVlMwPS9zMDFOwG0> NVe4bVlMOzBibXnu NXTGcnVWTE2VTx?= Mn\ZdJJmfmWwdIOgVGRITi1iYX7kJJRpem:vYnnuMY1m\GmjdHXkJHZUVUNicILvcIln\XKjdHnvckBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NGLsZWUzODh2N{OwOi=>
MDA-MB-231 MXXBdI9xfG:|aYOgRZN{[Xl? M{H0fVExKM7:TR?= MXuyOEBp MmPnSG1UV8Li MV7pcoR2[2W|IHHwc5B1d3Orcx?= MmrDNVcyOTRyMEW=
MDA-MB-435S NFLWTnFCeG:ydH;zbZMhSXO|YYm= NVX2S3pJOTBizszN NYfTNWViOjRiaB?= MlfNSG1UV8Li M2nwTIlv\HWlZYOgZZBweHSxc3nz M1TZcVE4OTF2MEC1

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-STAT3 / STAT3 ; 

PubMed: 25261365     


(A) PC3M-1E8 cells were treated with different doses of Stattic for 4 h. (B) PC3M-1E8 cells were treated with 10 μM Stattic for different time points. 

Survivin / c-Myc / Bcl-xl ; 

PubMed: 25261365     


Western blot analysis of STAT3 downstream targets in PC3M-1E8 cells treated with 10 μM Stattic for 24 h. 

PARP / C-PARP / Caspase-3 / C-Caspse-3 ; 

PubMed: 23382914     


NPC cells were exposed to the indicated concentrations of Stattic for 48 h; apoptotic cells were measured by western blot analysis of cleaved PARP and cleaved caspase-3. Protein levels were quantified using ImageJ software. 

25261365 23382914
Immunofluorescence
p-STAT3 / STAT3 / Survivin ; 

PubMed: 25261365     


Cellular localization of pSTAT3 and survivin (green) was observed by confocal microscopy after immunofluorescent staining of PC3M-1E8 cancer cells treated with 10 μM Stattic.

25261365
Growth inhibition assay
Cell viability; 

PubMed: 23382914     


Stattic inhibits the cell viability of NPC cells in a time-dependent manner. CNE2 cells were treated with 8 µM Stattic for the indicated times, and cell viability was measured by the MTT assay. 

23382914
ELISA
BDNF; 

PubMed: 27456333     


BDNF secretion was analyzed using ELISA in A549 and H1299 cells treated with or without Stattic. Results are shown as the mean ± S.E. n = 3; *p < 0.05, vs Control group, Student’s t-test. 

27456333

Protocol

Kinase Assay:

[1]

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High-Throughput Screening and FluorescencePolarization Assays:

Screening is performed at approximately 30 °C. The specificity ofscreening hits is validated in analogous assays for binding of thetest compounds to the SH2 domains of STAT1, STAT5, and Lck. The final concentration of buffer components used for all FP assays is 10 mM HEPES (pH 7.5), 1 mM EDTA, 0.1% Nonidet P-40, 50 mM NaCl, and 10% DMSO. The absence of dithiothreitol is essential for inhibitory activity. The sequences of the peptides are: STAT3, 5-carboxyfluorescein-GY(PO3H2)LPQTV-NH2; STAT1, 5-carboxyfluorescein-GY(PO3H2)DKPHVL; STAT5, 5-carboxyfluorescein-GY(PO3H2)LVLDKW; and Lck, 5-carboxyfluorescein-GY(PO3H2)EEIP. For specificity analysis at 30 °C, proteins are used at 150 nM (STAT1, STAT3, and STAT5). For specificity analysis at 37 °C, proteins are used at 370 nM (STAT3) or 100 nM (Lck). Proteins are incubated with test compounds in Eppendorf tubes at the indicated temperatures for 60 min prior addition of therespective 5-carboxyfluorescein labeled peptides (final concentration: 10 nM).Before measurement at room temperature, the mixtures are allowed to equilibrate for at least 30 min. Test compounds are used at the indicated concentrations diluted from a 20× stock in DMSO. Binding curves and inhibition curves are fitted with SigmaPlot. All competition curves are repeated three times in independent experiment
Cell Research:

[2]

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  • Cell lines: Ly3 cells
  • Concentrations: ~2.5 μM
  • Incubation Time: 48 h
  • Method:

    MTS


    (Only for Reference)
Animal Research:

[2]

- Collapse
  • Animal Models: Ly3 parental NOD/SCID IL2R null xenograft
  • Formulation: --
  • Dosages: 3.75 mg/kg every day
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 42 mg/mL (198.87 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+H2O
For best results, use promptly after mixing.
8mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 211.19
Formula

C8H5NO4S

CAS No. 19983-44-9
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    We need to dissolve the compound for i.p. injection, could you give me some suggestions?

  • Answer:

    S7024 Stattic can be dissolved in 2% DMSO/PEG 300/dd H2O at 8 mg/mL as a clear solution.

STAT Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID