Stattic

For research use only.

Catalog No.S7024

150 publications

Stattic Chemical Structure

CAS No. 19983-44-9

Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1. Stattic induces apoptosis.

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Selleck's Stattic has been cited by 150 publications

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Choose Selective STAT Inhibitors

Biological Activity

Description Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1. Stattic induces apoptosis.
Features Stattic is the first non-peptide small molecule with inhibitory activity against STAT3 SH2 domain regardless of the STAT3 phosphorylation state in vitro.
Targets
STAT3 [1]
(Cell-free assay)
5.1 μM
In vitro

Stattic inhibits binding of a phosphotyrosine-containing peptide derived from the gp130 receptor to the STAT3 SH2 domain in a strongly temperature-dependent manner. Stattic only has a very weak effect on binding of a tyrosinephosphorylated peptide to the SH2-domain of the tyrosine kinase Lck. And it doesn’t inhibit dimerization of two other dimeric transcription factors (c-Myc/Max and Jun/Jun). It also inhibits fluorescein-labeled phosphopeptides to the SH2 domains of STAT1 and STAT5b. Stattic selectively inhibits DNA binding of STAT3 homodimers at a concentration of 10 μM. Shown to inhibit cellular phosphorylation of STAT3 at Tyr705 with little effect towards STAT1 phosphorylation at Tyr701 (in HepG2 cells) or the phosphorylation of JAK1, JAK2, and c-Src (in MDA-MB-231 and MDA-MB-235S cells). Stattic increases the apoptotic rate of STAT3-dependent breast cancer cell lines. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H9c2 NWfCcldJTnWwY4Tpc44hSXO|YYm= M4\jUlEx6oDLzszN Mn\jOQKBkWh? M{TDcZJmfmW{c3XzJJRp\SCnZn\lZ5R{KG:oIFnMMVI4 MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN|OU[zN{c,OjZ|M{m2N|M9N2F-
NPC  M4e5Z2Z2dmO2aX;uJGF{e2G7 M{PVTlAuPy53INM1US=> M13EN4Fjd2yrc3jld{BGVVRvbHnr[UBud2ynY4XsZZIh[Wy2ZYLheIlwdnNuIHHu[EBk\WyuIH3p[5JifGmxbjDhcoQhcW64YYPpc44hcW6mdXPl[EBjgSCUS1nQJItvd2OtZH;3ci=> Ml;LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7MUW0N|AoRjJ3OUG1OFMxRC:jPh?=
HASMC M372dGZ2dmO2aX;uJGF{e2G7 NIDCWmoyNjJ3LUWg{txO NUHYTolbOjBibXnu NVO5WWlFTE2VT9Mg MnP1bY5pcWKrdIOgdE0pYSlvU2TBWE0yNDNuNTDzbYdv[Wy|wrC= MmrJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6NEm2NlIoRjJ3OES5OlIzRC:jPh?=
H9c2  NVnvT4VRTnWwY4Tpc44hSXO|YYm= NE\CNlczNzFyIN88US=> M1LmflIhcA>? M1TMeGROW00EoB?= NXPIW2Fz[WK{b3fheIV{yqC2aHWgZ5l1d3C{b4TlZ5RqfmViZX\m[YN1eyCxZjDJUE0zPyCjZ3HpcpN1KFOK M4nCeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEKwPVA4Lz5{NUiyNFkxPzxxYU6=
A431 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{fNUVLDqM7:TR?= MlfiNuKhcA>? NVPtSolS[myxY3vzJGVITi2{ZY\ldpNm\CCmZXPy[YF{\XNiaX6gZ4VtdCC4aXHibYxqfHl? Mk\mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5MkC0N|UoRjJ3N{KwOFM2RC:jPh?=
A431 NE\Pc3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEe0dYgzyqEQvF2= MoLkNuKhcA>? Mn34bY5kemWjc3XzJIlvKGGyb4D0c5NqeyCrbnT1Z4VlKGK7IIPobYtwdmmw NWPD[Y43RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3NlA1OzVpPkK1O|IxPDN3PD;hQi=>
SiHa M2rCcmNmdGxiVnnhZoltcXS7IFHzd4F6 NUHWe25rPS15NTDuUS=> NXvVdVdPOjUEoHlCpC=> NWDEUYVMe2ixd4OgcY9zeGixbH;nfUBw\iCjIIT5dIlk[WxiYYDvdJRwfGmlIHPlcIwh[W6mIHTvd4Uu\GWyZX7k[Y51KGyxc4Ogc4Yh[2WubDD2bYFjcWyrdIpCpC=> M{\VPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUO5OlQ1Lz5{NUWzPVY1PDxxYU6=
SiHa NIjCTVJHfW6ldHnvckBCe3OjeR?= Mnu5OU04PSCwTR?= M1LFd|I1yqCqwrC= M2XY[ZJm\HWlZYOgeIhmyqCyaH;zdIhwenmuYYTpc44h[XRidHjlJJR6em:|aX7lJJJme2mmdXWgO|A2yqB? MofUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3M{m2OFQoRjJ3NUO5OlQ1RC:jPh?=
ECA109 NEPtSHdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mlz1NE0zOCEQvF2= NU\WUJo6OjUEoHlCpC=> M2nI[mlEPTB;NT61NEDPxE1? M2jR[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEmyOFgxLz5{NUS5NlQ5ODxxYU6=
TE13 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXewMVIxKM7:TR?= MWqyOOKhcMLi NH7FSZdKSzVyPU[uNVXDqM7:TR?= NInw[4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS5NlQ5OCd-MkW0PVI1QDB:L3G+
KYSE150  NWSzSJp2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIT5bm0xNTJyIN88US=> NXfKS2xLOjUEoHlCpC=> NF;QUYFKSzVyPUGyMlY1yqEQvF2= MmDMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2OUK0PFAoRjJ3NEmyOFgxRC:jPh?=
ECA109 NVv0[JhQS2yxbn;n[Y5q[yCVdYL2bZZidCCDc4PhfS=> MW[wMlXDqM7:TR?= MVmyOOKhcMLi NEf0NIl{fXCycnXzd4V{KHSqZTDjcI9vd2enbnnjJIZwem2jdHnvci=> MlvCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2OUK0PFAoRjJ3NEmyOFgxRC:jPh?=
TE13 NX\HS4VCS2yxbn;n[Y5q[yCVdYL2bZZidCCDc4PhfS=> NFjPZmYxNjYEoN88US=> NV;nfHZLOjUEoHlCpC=> Mkjpd5VxeHKnc4Pld{B1cGViY3zvco9o\W6rYzDmc5Ju[XSrb36= NFvkbGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS5NlQ5OCd-MkW0PVI1QDB:L3G+
KYSE150  NUPUUFh3S2yxbn;n[Y5q[yCVdYL2bZZidCCDc4PhfS=> NUTJWopTOC53wrFOwG0> MmHaNlTDqGkEoB?= MWTzeZBxemW|c3XzJJRp\SClbH;uc4dmdmmlIH\vdo1ifGmxbh?= NHTtVpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS5NlQ5OCd-MkW0PVI1QDB:L3G+
ECA109 MnXwSpVv[3Srb36gRZN{[Xl? MmK3NE42yqEQvF2= MlTVNlTDqGkEoB?= NGXNTZNmdmijbnPld{BKWi2rbnT1Z4VlKGenbnXyZZRqd25ib3[gSHNDew>? NGmzdIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS5NlQ5OCd-MkW0PVI1QDB:L3G+
PC3M-1E8 M1znOWZ2dmO2aX;uJGF{e2G7 M1njZ|IvPS93L{GwJO69VQ>? MUmwMVQhcA>? NVP2bHVHcW6qaXLpeJMhfGinIGPURXQ{KGGldHn2ZZRqd25iaX6gZUBld3OnLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{wrC= NEeySlA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK2NVM3PSd-MkWyOlE{PjV:L3G+
PC3M-1E8 NVL3SndETnWwY4Tpc44hSXO|YYm= Ml61NVAh|ryP M4\oVlI1KGh? M3jjUYRwf26{ZXf1cIF1\XNiQnPsMZhNNCC|dYL2bZZqdiCjbnSgZ{1OgWN? NY[yS|hiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyOlE{PjVpPkK1NlYyOzZ3PD;hQi=>
PC3M-1E8 MlHHSpVv[3Srb36gRZN{[Xl? NHHlZ2YyOCEQvF2= MnLpNlQhcA>? MVzpcohq[mm2czDJUE03KGmwZIXj[YQhW1SDVEOgZYN1cX[jdHnvckBidmRidHjlJGlNNTZvaX7keYNm\CCVVFHUN{Bi[3SrdnH0bY9v NVS3elhkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyOlE{PjVpPkK1NlYyOzZ3PD;hQi=>
PC3M-1E8 MWLDcI9vd2enbnnjJHN2en[rdnHsJGF{e2G7 MnPENk42NzVxMUCg{txO MX3pcohq[mm2c9MgeIhmKGOxbH;ufUBnd3KvYYTpc44he2mpbnnmbYNidnSueR?= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ4MUO2OUc,OjV{NkGzOlU9N2F-
MDA-MB-231 NUnP[o1MTnWwY4Tpc44hSXO|YYm= M1\GU|IxKM7:TR?= NH;TNFQzKGh? Mkjj[ZhpcWKrdIOgV45icWxiYX7kJGUu[2GmaHXybY4h\XiycnXzd4lwdg>? M1\PNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUWzN|Q6Lz5{NUG1N|M1QTxxYU6=
H9c2 M1rDOmZ2dmO2aX;uJGF{e2G7 M4rJclIxKML3TR?= NFLsXpQ{OCCvaX6= NEG5[JpFVVORwrC= NEm4e5Ni[m:uaYPo[ZMheHKxcH;mc4wucW6mdXPl[EBCU1RicHjvd5Bpd3K7bHH0bY9vKGG2IHLveIghe2W{NEezJIFv\CC2aIKzNFg> NF34ToU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGwOVA3Pyd-MkWxNFUxPjd:L3G+
HaCaT  NGjvbnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFrnVoIyOCEEtV2= MmjtNlAhdWmw NF;GUGJFVVORwrC= NGnpbnJmdmijbnPld{B{d3KjZnXubYIuKGGwZDDzeY5qfGmwaXKtbY5lfWOnZDDndo94fGhiaX7obYJqfGmxbh?= NWLmSnB{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwNVM6ODdpPkK1NFE{QTB5PD;hQi=>
Caki-1 NHzFSm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\UNlExKML3TR?= NFfye3gzOCCvaX6= MmXjSG1UV8Li M33yVYVvcGGwY3XzJJNwemGoZX7pZk0h[W6mIIP1col1cW6rYj3pcoR2[2WmIHfyc5d1cCCrbnjpZol1cW:w MofsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyMUO5NFcoRjJ3MEGzPVA4RC:jPh?=
HaCaT  Moq5RZBweHSxc3nzJGF{e2G7 MXmxNEDDvU1? NHnRT4YzOCCvaX6= M1;s[mROW00EoB?= MU\pcoNz\WG|ZYOgdJJweG:{dHnvcpMhd2ZiYYDvdJRwfGmlIHPlcIx{KGS3ZTD0c{B1emWjdH3lcpQhf2m2aDDzc5Ji\mWwaXKgc5Ihe3WwaYTpcolj MmD4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyMUO5NFcoRjJ3MEGzPVA4RC:jPh?=
FHL-primed hNSCs MXzD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MUSwMlAzNTVizszN NEfv[FU4OiCq NV\pcXo1dGWjZIOgeI8hfGinIHzvd5Mhd2ZiY3XscEB3cWGkaXzpeJkh[XRiaHnnbEBkd26lZX70doF1cW:w NGDLbG09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm0OVQ{PCd-MkS5OFU1OzR:L3G+
ELL-primed hNSCs NEXzeINE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NIi3OFExNjB{LUWg{txO NEXrZ|U4OiCq MYrs[YFleyC2bzD0bIUhdG:|czDv[kBk\WyuII\pZYJqdGm2eTDheEBpcWeqIHPvcoNmdnS{YYTpc44> MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl2NUSzOEc,OjR7NEW0N|Q9N2F-
SS NG\5dXZE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NWPzXHREOS1zMDFOwG0> M3nOblczKGh? M1vPXmROW09? MoHDZ4F2e2W|IHGg[I9{\S2mZYDlcoRmdnRiaX7obYJqfGmxbjDv[kB1cGVidnnhZoltcXS7wrC= NV:yN3NlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3OVYyOTFpPkK0O|U3OTFzPD;hQi=>
SeAx  MmfnR4VtdCCYaXHibYxqfHliQYPzZZk> NYL2dnF2OS1zMDFOwG0> NYHSRZVjPzJiaB?= NXzHSZhHTE2VTx?= NF\vbWtk[XW|ZYOgZUBld3OnLXTldIVv\GWwdDDpcohq[mm2aX;uJI9nKHSqZTD2bYFjcWyrdIpCpC=> M13ETFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{W2NVEyLz5{NEe1OlEyOTxxYU6=
HuT-78 NULMWlU5S2WubDDWbYFjcWyrdImgRZN{[Xl? Mn[zNU0yOCEQvF2= NGPTWGQ4OiCq M1PKd2ROW09? NV\K[pZD[2G3c3XzJIEh\G:|ZT3k[ZBmdmSnboSgbY5pcWKrdHnvckBw\iC2aHWgeoli[mmuaYT5xsA> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd3NkGxNUc,OjR5NU[xNVE9N2F-
CD4+ M2fwZmFxd3C2b4Ppd{BCe3OjeR?= NIC2VI4yOOLCid88cS=> NGO4RYczPCCq NXrzNnJFTE2VTx?= NWjaUoV7cW6mdXPld{BieG:ydH;zbZMhe3S{b37ncJk> MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd3NkGxNUc,OjR5NU[xNVE9N2F-
MCF-7 M3WzUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoLRNE41PjlvMz63OUDPxE1? NICxem42KGR? MWny[YR2[2W|IHPlcIwhdnWvYnXyJJNq\26rZnnjZY51dHl? MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd{OEC3PEc,OjR5MkiwO|g9N2F-
MCF-7/LCC1 Mn;LS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWSzdmI6OC52NkmtN{44PSEQvF2= M1fOT|Uh\A>? M2GydZJm\HWlZYOgZ4VtdCCwdX3i[ZIhe2mpbnnmbYNidnSueR?= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd{OEC3PEc,OjR5MkiwO|g9N2F-
MCF-7/LCC9 NXrxRlVWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWewMlQ3QS1|Lke1JO69VQ>? NF3QRpo2KGR? MVny[YR2[2W|IHPlcIwhdnWvYnXyJJNq\26rZnnjZY51dHl? M1jBeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{K4NFc5Lz5{NEeyPFA4QDxxYU6=
HaCaT MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEKzbIIyOCEEtV2= MorHNlAhdWmw NEfsOWxFVVORwrC= M{C3bYVvcGGwY3XzJIV3\XKxbHnteZMucW6mdXPl[EBk\WyuIHfyc5d1cCCrbnjpZol1cW:w MlrKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2MkOxN|EoRjJ2NEKzNVMyRC:jPh?=
HaCaT NGPucJpCeG:ydH;zbZMhSXO|YYm= NVvLUmlnOTBiwsXN MWCyNEBucW5? MWnEUXNQyqB? M4nnXYVvcGGwY3XzJJRp\SCjcH;weI91cWNiZX\m[YN1eyCxZjDleoVzd2yrbYXz MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR{M{GzNUc,OjR2MkOxN|E9N2F-
MDA-MB-231 MVHGeY5kfGmxbjDBd5NigQ>? M3LqbFExKML3TR?= NEXLOm0zPCCq M1jMTGROW00EoB?= NWriS|FYemWmdXPld{BRNVOWQWSzJIV5eHKnc4Ppc44> NHHhd3A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO3OlU5Pid-MkSzO|Y2QDZ:L3G+
SUM-159 M1P3TWZ2dmO2aX;uJGF{e2G7 NF7E[o8yOCEEtV2= M1[1blI1KGh? MlfzSG1UV8Li M{XRZZJm\HWlZYOgVE1UXEGWMzDlfJBz\XO|aX;u M2XReFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{e2OVg3Lz5{NEO3OlU5PjxxYU6=
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MDA-MB-231 MUjBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? Ml;3O|IhcHK| M2rvSWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgSXIudmWpYYTpeoUh[W6mIITybZBt\S2wZXfheIl3\SCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gZ4VtdCCycn;sbYZmemG2aX;uJIFnfGW{IEeyJIhzeyCkeTDNWHMh[XO|YYmsJGlEPTBiPTCyMlg6KM7:TT6= NWDH[4lnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFQ6PjZpPkK0PVA1QTZ4PD;hQi=>
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MDA-MB-435S Ml\hR5l1d3SxeHnjbZR6KGG|c3H5 NW\aZmpDPDhiaILz NIP5dIREgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtOFM2WyClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOS56NzFOwG0v M2LK[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m2Olg6Lz5{NkO5OlY5QTxxYU6=
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HCT116 MmnWRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M{HpNVczKGi{cx?= M2ToRWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFPUNVE3KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCxMlA5KM7:TT6= NX75TXlxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke3NVg1PzBpPkK3O|E5PDdyPD;hQi=>
MDA-MB-231 NV\vbXlvSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MX:3NkBpenN? MXPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFEvPjhizszNMi=> NX7tRpRCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke3NVg1PzBpPkK3O|E5PDdyPD;hQi=>
MCF7 MXLBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NFLPdm44OiCqcoO= M36zdGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMj6zOkDPxE1w NWq5NmlmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke3NVg1PzBpPkK3O|E5PDdyPD;hQi=>
A549 M2TyXGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MUO3NkBpenN? NHHld|RCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IESuOEDPxE1w MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzdzOES3NEc,Ojd5MUi0O|A9N2F-
AD293 M2nRfGZ2dmO2aX;uJIF{e2G7 NFX4Wok3KGi{cx?= NUnJcXNDUW6qaXLpeIlwdiCxZjDJSm5o[W2vYT3zeIlufWyjdHXkJGdHWC:ITFHHMZRi\2enZDDTWGFVOyCmaX3ldol7[XSrb36gbY4hcHWvYX6gRWQzQTNiY3XscJMhcW6ldXLheIVlKG[xcjC2JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZMtKEmFNUCgQUA2NjFizszNMi=> NUL4RXFHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyNlgxODBpPkOwNlI5ODByPD;hQi=>
MDA-MB-231 M4H1XGZ2dmO2aX;uJIF{e2G7 M4n6fVEhfG9iMUCgeW0> NF62fG4yOiCqcoO= Mo[yTY5pcWKrdHnvckBw\iCVVFHUN{BxcG:|cHjvdplt[XSrb36gZZQhXHm{N{C1JIlvKGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZZQhOSC2bzCxNEB2VSCjZoTldkAyOiCqcoOgZpkhf2W|dHXyckBjdG:2IHHuZYx6e2m| NIWyZo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwOFk3Pid-MkS5NFQ6PjZ:L3G+
MDA-MB-231 MmLvRY51cWOjbnPldkBie3OjeR?= Mn22NUB1dyBzMDD1US=> MUe0PEBpenN? M3HvSmFvfGmlYX7j[ZIh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHzd4V{e2WmIHHzJINmdGxiZ4Lve5RpKGmwaHnibZRqd25uIHHwc5B1d3OrczDhcoQh[2WubIXsZZIhdW:{cHjvcI9ocWOjbDDjbIFv\2W|IHH0JFEhfG9iMUCgeW0h[W[2ZYKgOFghcHK|IHL5JIxq\2i2IH3pZ5Jwe2OxcIm= NEj4e249[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwOFk3Pid-MkS5NFQ6PjZ:L3G+
MDA-MB-231 NVzvVY5lTnWwY4Tpc44h[XO|YYm= NY[2PZNYOSC2bzCxNEB2VQ>? NW\NXY5XOTJiaILz MnrtSIVkemWjc3WgbY4hW1SDVEOgdJJwfGWrbjDlfJBz\XO|aX;uJIlvKGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZZQhOSC2bzCxNEB2VSCjZoTldkAyOiCqcoOgZpkhf2W|dHXyckBjdG:2IHHuZYx6e2m| MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyNEm2Okc,OjR7MES5OlY9N2F-
MCF7 MVrGeY5kfGmxbjDhd5NigQ>? NF;Ke48yOiCqcoO= M3fJeGlvcGmkaYTpc44hd2ZiU2TBWFMheGixc4Doc5J6dGG2aX;uJIF1KFl5MEWgbY4hcHWvYX6gUWNHPyClZXzsd{Bi\nSncjCxNkBpenNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ MoW2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OU[2PFkoRjJ4M{m2Olg6RC:jPh?=
MDA-MB-435S MoHaSpVv[3Srb36gZZN{[Xl? NEXBOJUyOiCqcoO= MYjJcohq[mm2aX;uJI9nKFOWQWSzJJBpd3OyaH;yfYxifGmxbjDheEB[PzB3IHnuJIh2dWGwIF3ERU1OSi12M{XTJINmdGy|IHHmeIVzKDF{IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= NXTGN2NJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVY3QDlpPkK2N|k3Pjh7PD;hQi=>
MDA-MB-231 MofGSpVv[3Srb36gZZN{[Xl? M37VS|EzKGi{cx?= MoXKTY5pcWKrdHnvckBw\iCVVFHUN{BxcG:|cHjvdplt[XSrb36gZZQhYTdyNTDpckBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHmeIVzKDF{IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= M1WxbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m2Olg6Lz5{NkO5OlY5QTxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-STAT3 / STAT3 ; 

PubMed: 25261365     


(A) PC3M-1E8 cells were treated with different doses of Stattic for 4 h. (B) PC3M-1E8 cells were treated with 10 μM Stattic for different time points. 

Survivin / c-Myc / Bcl-xl ; 

PubMed: 25261365     


Western blot analysis of STAT3 downstream targets in PC3M-1E8 cells treated with 10 μM Stattic for 24 h. 

PARP / C-PARP / Caspase-3 / C-Caspse-3 ; 

PubMed: 23382914     


NPC cells were exposed to the indicated concentrations of Stattic for 48 h; apoptotic cells were measured by western blot analysis of cleaved PARP and cleaved caspase-3. Protein levels were quantified using ImageJ software. 

25261365 23382914
Immunofluorescence
p-STAT3 / STAT3 / Survivin ; 

PubMed: 25261365     


Cellular localization of pSTAT3 and survivin (green) was observed by confocal microscopy after immunofluorescent staining of PC3M-1E8 cancer cells treated with 10 μM Stattic.

25261365
Growth inhibition assay
Cell viability; 

PubMed: 23382914     


Stattic inhibits the cell viability of NPC cells in a time-dependent manner. CNE2 cells were treated with 8 µM Stattic for the indicated times, and cell viability was measured by the MTT assay. 

23382914
ELISA
BDNF; 

PubMed: 27456333     


BDNF secretion was analyzed using ELISA in A549 and H1299 cells treated with or without Stattic. Results are shown as the mean ± S.E. n = 3; *p < 0.05, vs Control group, Student’s t-test. 

27456333

Protocol

Kinase Assay:

[1]

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High-Throughput Screening and FluorescencePolarization Assays:

Screening is performed at approximately 30 °C. The specificity ofscreening hits is validated in analogous assays for binding of thetest compounds to the SH2 domains of STAT1, STAT5, and Lck. The final concentration of buffer components used for all FP assays is 10 mM HEPES (pH 7.5), 1 mM EDTA, 0.1% Nonidet P-40, 50 mM NaCl, and 10% DMSO. The absence of dithiothreitol is essential for inhibitory activity. The sequences of the peptides are: STAT3, 5-carboxyfluorescein-GY(PO3H2)LPQTV-NH2; STAT1, 5-carboxyfluorescein-GY(PO3H2)DKPHVL; STAT5, 5-carboxyfluorescein-GY(PO3H2)LVLDKW; and Lck, 5-carboxyfluorescein-GY(PO3H2)EEIP. For specificity analysis at 30 °C, proteins are used at 150 nM (STAT1, STAT3, and STAT5). For specificity analysis at 37 °C, proteins are used at 370 nM (STAT3) or 100 nM (Lck). Proteins are incubated with test compounds in Eppendorf tubes at the indicated temperatures for 60 min prior addition of therespective 5-carboxyfluorescein labeled peptides (final concentration: 10 nM).Before measurement at room temperature, the mixtures are allowed to equilibrate for at least 30 min. Test compounds are used at the indicated concentrations diluted from a 20× stock in DMSO. Binding curves and inhibition curves are fitted with SigmaPlot. All competition curves are repeated three times in independent experiment
Cell Research:

[2]

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  • Cell lines: Ly3 cells
  • Concentrations: ~2.5 μM
  • Incubation Time: 48 h
  • Method:

    MTS


    (Only for Reference)
Animal Research:

[2]

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  • Animal Models: Ly3 parental NOD/SCID IL2R null xenograft
  • Dosages: 3.75 mg/kg every day
  • Administration: intra tumor
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 42 mg/mL (198.87 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O
For best results, use promptly after mixing.
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 211.19
Formula

C8H5NO4S

CAS No. 19983-44-9
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC(=CC2=C1C=CS2(=O)=O)[N+](=O)[O-]

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    We need to dissolve the compound for i.p. injection, could you give me some suggestions?

  • Answer:

    S7024 Stattic can be dissolved in 2% DMSO/PEG 300/dd H2O at 8 mg/mL as a clear solution.

STAT Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID