Stattic

For research use only.

Catalog No.S7024

99 publications

Stattic Chemical Structure

Molecular Weight(MW): 211.19

Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1. Stattic induces apoptosis.

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Selleck's Stattic has been cited by 99 publications

Purity & Quality Control

Choose Selective STAT Inhibitors

Biological Activity

Description Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1. Stattic induces apoptosis.
Features Stattic is the first non-peptide small molecule with inhibitory activity against STAT3 SH2 domain regardless of the STAT3 phosphorylation state in vitro.
Targets
STAT3 [1]
(Cell-free assay)
5.1 μM
In vitro

Stattic inhibits binding of a phosphotyrosine-containing peptide derived from the gp130 receptor to the STAT3 SH2 domain in a strongly temperature-dependent manner. Stattic only has a very weak effect on binding of a tyrosinephosphorylated peptide to the SH2-domain of the tyrosine kinase Lck. And it doesn’t inhibit dimerization of two other dimeric transcription factors (c-Myc/Max and Jun/Jun). It also inhibits fluorescein-labeled phosphopeptides to the SH2 domains of STAT1 and STAT5b. Stattic selectively inhibits DNA binding of STAT3 homodimers at a concentration of 10 μM. Shown to inhibit cellular phosphorylation of STAT3 at Tyr705 with little effect towards STAT1 phosphorylation at Tyr701 (in HepG2 cells) or the phosphorylation of JAK1, JAK2, and c-Src (in MDA-MB-231 and MDA-MB-235S cells). Stattic increases the apoptotic rate of STAT3-dependent breast cancer cell lines. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H9c2 MlHpSpVv[3Srb36gRZN{[Xl? NWH6fHJ[OTEkgJpOwG0> M2DzdFTjiImq NV[2TlZGemW4ZYLz[ZMhfGinIHXm[oVkfHNib3[gTWwuOjd? MlryNlY{Ozl4M{O=
NPC  NXrIWXJHTnWwY4Tpc44hSXO|YYm= NHu0OVUxNTdwNTFCuW0> MXPhZo9tcXOqZYOgSW1VNWyra3WgcY9t\WO3bHHyJIFtfGW{YYTpc45{NCCjbnSgZ4VtdCCvaXfyZZRqd25iYX7kJIlvfmG|aX;uJIlv\HWlZXSgZpkhWkuLUDDrco9kc2Sxd36= NUfMWndEOjV7MUW0N|A>
HASMC NVfTSGdkTnWwY4Tpc44hSXO|YYm= NVLleIxiOS5{NT21JO69VQ>? M1rncVIxKG2rbh?= M4P0eWROW00EoB?= MV\pcohq[mm2czDwMUh[MS2VVFHUMVEtOyx3IIPp[45idHQEoB?= M1G1bFI2QDR7NkKy
H9c2  MWfGeY5kfGmxbjDBd5NigQ>? MmHnNk8yOCEQvF2= MojENkBp MUfEUXNQyqB? MYfhZpJw\2G2ZYRCpJRp\SCleYTvdJJwfGWldHn2[UBm\m[nY4TzJI9nKEmOLUK3JIFo[Wmwc4SgV2g> NYTDfHE1OjV6MkC5NFc>
A431 NWTlSHg6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2nO[lLDqM7:TR?= NH\J[XczyqCq MVzicI9kc3NiRVfGMZJmfmW{c3XkJIRm[3KnYYPld{BqdiClZXzsJJZq[WKrbHn0fS=> Mlu3NlU4OjB2M{W=
A431 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYXUWFU{OsLizszN MknFNuKhcA>? MXTpcoNz\WG|ZYOgbY4h[XCxcITvd4l{KGmwZIXj[YQh[nlic3jpb49vcW5? M3\hUFI2PzJyNEO1
SiHa NVfCNZlRS2WubDDWbYFjcWyrdImgRZN{[Xl? MUe1MVc2KG6P M4PEXlI1yqCqwrC= NXrYO2ZLe2ixd4OgcY9zeGixbH;nfUBw\iCjIIT5dIlk[WxiYYDvdJRwfGmlIHPlcIwh[W6mIHTvd4Uu\GWyZX7k[Y51KGyxc4Ogc4Yh[2WubDD2bYFjcWyrdIpCpC=> M3z5RVI2PTN7NkS0
SiHa NIXxbI9HfW6ldHnvckBCe3OjeR?= MmHoOU04PSCwTR?= M3TjfVI1yqCqwrC= MWPy[YR2[2W|IITo[eKheGixc4Doc5J6dGG2aX;uJIF1KHSqZTD0fZJwe2mwZTDy[ZNq\HWnIEewOeKh NIT5WYUzPTV|OU[0OC=>
ECA109 M4\VeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3zxblAuOjBizszN M1PLTVI1yqCqwrC= NHzsT2xKSzVyPUWuOVAh|ryP MoXnNlU1QTJ2OEC=
TE13 MlPJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWGwMVIxKM7:TR?= MYWyOOKhcMLi MmDMTWM2OD14LkG1xsDPxE1? M4WzflI2PDl{NEiw
KYSE150  MkjiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4qzV|AuOjBizszN M{H6ZVI1yqCqwrC= M3\Y[mlEPTB;MUKuOlTDqM7:TR?= NHX3bpIzPTR7MkS4NC=>
ECA109 NETSPGdEdG:wb3flcolkKFO3co\peoFtKEG|c3H5 NXnscVhNOC53wrFOwG0> NHjac|EzPMLiaNMg M1\xV5N2eHC{ZYPz[ZMhfGinIHPsc45w\2WwaXOg[o9zdWG2aX;u MnzONlU1QTJ2OEC=
TE13 M1nLXGNtd26xZ3XubYMhW3W{dnn2ZYwhSXO|YYm= Mlv2NE42yqEQvF2= MVSyOOKhcMLi NEPrcY9{fXCycnXzd4V{KHSqZTDjcI9vd2enbnnjJIZwem2jdHnvci=> MoLzNlU1QTJ2OEC=
KYSE150  NIm3Oo1EdG:wb3flcolkKFO3co\peoFtKEG|c3H5 MVWwMlXDqM7:TR?= NWfNZppROjUEoHlCpC=> NVzsbZpCe3WycILld5NmeyC2aHWgZ4xwdm:pZX7pZ{Bnd3KvYYTpc44> NWXLOnh3OjV2OUK0PFA>
ECA109 M{S5[2Z2dmO2aX;uJGF{e2G7 NF3JSmwxNjYEoN88US=> MUCyOOKhcMLi MXXlcohidmOnczDJVk1qdmS3Y3XkJIdmdmW{YYTpc44hd2ZiRGPCdy=> MVyyOVQ6OjR6MB?=
PC3M-1E8 NH75UXFHfW6ldHnvckBCe3OjeR?= NWj2R3dDOi53L{WvNVAh|ryP NYW2c5pVOC12IHi= M{HYeYlvcGmkaYTzJJRp\SCVVFHUN{Bi[3SrdnH0bY9vKGmwIHGg[I9{\S1iYX7kJJRqdWVvZHXw[Y5l\W62IH3hco5mesLi NUfYToxWOjV{NkGzOlU>
PC3M-1E8 NWfNTGs6TnWwY4Tpc44hSXO|YYm= M{\kRVExKM7:TR?= M2GxPFI1KGh? NXvWNWtV\G:5boLl[5Vt[XSnczDCZ4wugExuIIP1dpZqfmmwIHHu[EBkNU27Yx?= MmLCNlUzPjF|NkW=
PC3M-1E8 MkiySpVv[3Srb36gRZN{[Xl? MnW1NVAh|ryP M2PlXFI1KGh? NGXtc4NqdmirYnn0d{BKVC14IHnu[JVk\WRiU2TBWFMh[WO2aY\heIlwdiCjbnSgeIhmKEmOLU[tbY5lfWOnZDDTWGFVOyCjY4TpeoF1cW:w MUmyOVI3OTN4NR?=
PC3M-1E8 M4Ha[2Ntd26xZ3XubYMhW3W{dnn2ZYwhSXO|YYm= MkPRNk42NzVxMUCg{txO MXzpcohq[mm2c9MgeIhmKGOxbH;ufUBnd3KvYYTpc44he2mpbnnmbYNidnSueR?= NVy2VWFtOjV{NkGzOlU>
MDA-MB-231 MoDkSpVv[3Srb36gRZN{[Xl? NYruPHZUOjBizszN MnvuNkBp NWfucHIz\XiqaXLpeJMhW26jaXygZY5lKEVvY3HkbIVzcW5iZYjwdoV{e2mxbh?= NVftfHNiOjVzNUOzOFk>
H9c2 NV7FSVhQTnWwY4Tpc44hSXO|YYm= MUiyNEDDvU1? MUGzNEBucW5? NX\xS5UzTE2VT9Mg MUPhZo9tcXOqZYOgdJJweG:ob3ytbY5lfWOnZDDBT3QheGixc4Doc5J6dGG2aX;uJIF1KGKxdHigd4VzPDd|IHHu[EB1cHJ|MEi= NGLoS4IzPTFyNUC2Oy=>
HaCaT  MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NInTNGEyOCEEtV2= NFvN[5czOCCvaX6= MYnEUXNQyqB? M{e0dYVvcGGwY3XzJJNwemGoZX7pZk0h[W6mIIP1col1cW6rYj3pcoR2[2WmIHfyc5d1cCCrbnjpZol1cW:w M1rDfVI2ODF|OUC3
Caki-1 M2H4Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXmxNEDDvU1? MXmyNEBucW5? Mn3iSG1UV8Li MkTO[Y5p[W6lZYOgd49z[W[nbnniMUBidmRic4XubZRqdmmkLXnu[JVk\WRiZ4Lve5RpKGmwaHnibZRqd25? MXGyOVAyOzlyNx?=
HaCaT  M4X4SmFxd3C2b4Ppd{BCe3OjeR?= NIXRfZEyOCEEtV2= M3zFT|IxKG2rbh?= NXm5cId{TE2VT9Mg NYjDZZdYcW6lcnXhd4V{KHC{b4DvdpRqd26|IH;mJIFxd3C2b4TpZ{Bk\WyuczDkeYUhfG9idILlZZRu\W62IIfpeIghe2:{YX\lcoljKG:{IIP1col1cW6rYh?= M{TNPVI2ODF|OUC3
FHL-primed hNSCs NHu4b4tE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NGj5[5kxNjB{LUWg{txO NH\ycW84OiCq MV7s[YFleyC2bzD0bIUhdG:|czDv[kBk\WyuII\pZYJqdGm2eTDheEBpcWeqIHPvcoNmdnS{YYTpc44> MXmyOFk1PTR|NB?=
ELL-primed hNSCs Mlu0R4VtdCCYaXHibYxqfHliQYPzZZk> M1LZS|AvODJvNTFOwG0> MonzO|IhcA>? M4\CUoxm[WS|IITvJJRp\SCub4PzJI9nKGOnbHygeoli[mmuaYT5JIF1KGirZ3igZ49v[2WwdILheIlwdg>? NITKWZgzPDl2NUSzOC=>
SS MmjqR4VtdCCYaXHibYxqfHliQYPzZZk> M2DKSFEuOTBizszN M4HqOVczKGh? MV;EUXNQ M3\nRoNifXOnczDhJIRwe2VvZHXw[Y5l\W62IHnubIljcXSrb36gc4YhfGinII\pZYJqdGm2edMg M373cVI1PzV4MUGx
SeAx  NGDQNmtE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MWSxMVExKM7:TR?= MojCO|IhcA>? MUHEUXNQ NGW4bndk[XW|ZYOgZUBld3OnLXTldIVv\GWwdDDpcohq[mm2aX;uJI9nKHSqZTD2bYFjcWyrdIpCpC=> Mmn3NlQ4PTZzMUG=
HuT-78 M1vhemNmdGxiVnnhZoltcXS7IFHzd4F6 M4XMdVEuOTBizszN MYS3NkBp MYrEUXNQ NYf2WIxu[2G3c3XzJIEh\G:|ZT3k[ZBmdmSnboSgbY5pcWKrdHnvckBw\iC2aHWgeoli[mmuaYT5xsA> NUTafINmOjR5NU[xNVE>
CD4+ MornRZBweHSxc3nzJGF{e2G7 MUKxNQKBkc7:bR?= NV\ORnlHOjRiaB?= MWPEUXNQ NGjyN3JqdmS3Y3XzJIFxd3C2b4Ppd{B{fHKxbnfsfS=> MlHiNlQ4PTZzMUG=
MCF-7 NX\JZ5pUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI[zT3IxNjR4OT2zMlc2KM7:TR?= NXrDOXlUPSCm M3zN[5Jm\HWlZYOgZ4VtdCCwdX3i[ZIhe2mpbnnmbYNidnSueR?= M3zMUlI1PzJ6MEe4
MCF-7/LCC1 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFPJfm0xNjR4OT2zMlc2KM7:TR?= MmSxOUBl Moj5doVlfWOnczDj[YxtKG63bXLldkB{cWewaX\pZ4FvfGy7 Mn2wNlQ4OjhyN{i=
MCF-7/LCC9 M{\xVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVewMlQ3QS1|Lke1JO69VQ>? Mk\vOUBl M4XuRZJm\HWlZYOgZ4VtdCCwdX3i[ZIhe2mpbnnmbYNidnSueR?= MmD5NlQ4OjhyN{i=
HaCaT NYDsPItNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYHlcGpPOTBiwsXN MYmyNEBucW5? MnTmSG1UV8Li MoO3[Y5p[W6lZYOg[ZZmem:uaX31d{1qdmS3Y3XkJINmdGxiZ4Lve5RpKGmwaHnibZRqd25? M3TkVFI1PDJ|MUOx
HaCaT MkS2RZBweHSxc3nzJGF{e2G7 MlXhNVAhyrWP NEjuPWQzOCCvaX6= NHnYd3FFVVORwrC= MXTlcohidmOnczD0bIUh[XCxcITveIlkKGWoZnXjeJMhd2ZiZY\ldo9tcW23cx?= M2HJdlI1PDJ|MUOx
MDA-MB-231 NFfGdHBHfW6ldHnvckBCe3OjeR?= MVexNEDDvU1? NEPiNFQzPCCq NIH5coVFVVORwrC= MVHy[YR2[2W|IGCtV3RCXDNiZYjwdoV{e2mxbh?= NYn4Nmw1OjR|N{[1PFY>
SUM-159 MUfGeY5kfGmxbjDBd5NigQ>? MmLINVAhyrWP MkP6NlQhcA>? NFPRS5lFVVORwrC= M4e5epJm\HWlZYOgVE1UXEGWMzDlfJBz\XO|aX;u NVG3em1kOjR|N{[1PFY>
SK-BR-3 NWXzelNmTnWwY4Tpc44hSXO|YYm= MnK4NVAhyrWP M4i1bVI1KGh? Mn3vSG1UV8Li NIDXbGRz\WS3Y3XzJHAuW1SDVEOg[ZhxemW|c3nvci=> NVLv[4V4OjR|N{[1PFY>
MCF7-HER2 M1;hZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHmzTYMxNTFyIN88US=> M1izb|Q5KGh? MUXEUXNQ NVPI[lVJcW6mdXPld{Bk\WyuIHTlZZRpKGSxc3Wg[IVx\W6mZX70cJk> MV[yOFI6PzVyOB?=
MCF7-HER2 MoW3SpVv[3Srb36gRZN{[Xl? Ml3LOUDPxE1? NXXDT49jOjRiaB?= MUXEUXNQ MnX5[IlucW6rc3jld{BUd3hvMjygU4N1NTRuIHHu[EB{dHWpIHX4dJJme3Orb36= MX6yOFI6PzVyOB?=
MCF7-HER2 NXvFXIc3TnWwY4Tpc44hSXO|YYm= NXfZeFdlPSEQvF2= NEnFVnIzPCCq MYDEUXNQ Mkew[IVkemWjc3XzJJRp\SCneIDy[ZN{cW:wIHzleoVteyCxZjDFUXQhdWG{a3Xyd{whfmmvZX70bY4h[W6mIIPseYc> MoTjNlQzQTd3MEi=
MCF7-HER2 M2faUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn\uOUDPxE1? MoSyNlQhcA>? NXTIRYpoTE2VTx?= MYflcohidmOnczDj[YxtKGe{b4f0bEBqdmirYnn0bY9vKGOxbXLpcoVlKHerdHigTIVz[2WydHnu NIDNcJMzPDJ7N{WwPC=>
HMECs  M1rpb2Z2dmO2aX;uJGF{e2G7 NWW4XI02OTBizszN MV2yJIg> M4HSbolvcGmkaYTzJGlHVs7zwrDt[YRq[XSnZDDwbI9{eGixconsZZRqd25ib3[gV3RCXDFuIGPURXQzKGGwZDDTWGFVOw>? NEj5d4gzPDJzMUOyOy=>
HTR8/SVneo NGHzXJJHfW6ldHnvckBCe3OjeR?= M3P3eVHDqM7:TdMg NUnOSJREOcLiaB?= M33xNpN2eHC{ZYPz[YQhV1OPLXnu[JVk\WRiU2TBWFMheGixc4Doc5J6dGG2aX;u MlLlNlQxPjB{NEG=
HTR8/SVneo M2H1R2Z2dmO2aX;uJGF{e2G7 NWnMVIxXOC53L{JCpO69VcLi MlXnOFghcA>? NHq2cXBz\XO2b4Lld{B1cGViZYjwdoV{e2mxbjDv[kBGNWOjZHjldolvKHO3cIDy[ZN{\WRiYomgU3NO NYHUNGk3OjRyNkCyOFE>
HTR8/SVneo MlXOSpVv[3Srb36gRZN{[Xl? MoLWNeKh|ryPwrC= NUXlOpZWPDhiaB?= M2LNZpNq\26rZnnjZY51dHliaX7jdoVie2W|IH3p[5JifGmxbjDifUBQW04EoB?= MoHxNlQxPjB{NEG=
C13* NI\UdIhCeG:ydH;zbZMhSXO|YYm= M4XBWFAuOTBizszN NX;ud3FoOjRxNEigbC=> MlfabY5lfWOnczDhdI9xfG:|aYOgbY4h[SCmb4PlJIFv\CC2aX3lJIRmeGWwZHXueEBu[W6wZYK= M3y0XlI{QTZ{NUW4
OV2008 NYrKUYNOSXCxcITvd4l{KEG|c3H5 MXuwMVExKM7:TR?= NYPiTWVXOjRxNEigbC=> NYrDO2VycW6mdXPld{BieG:ydH;zbZMhcW5iYTDkc5NmKGGwZDD0bY1mKGSncHXu[IVvfCCvYX7u[ZI> MnLMNlM6PjJ3NUi=
C13* MX;BdI9xfG:|aYOgRZN{[Xl? NF7JRXkzPC92ODDo M37BPIVvcGGwY3XzJINqe3CuYYTpck1qdmS3Y3XkJIFxd3C2b4Ppdy=> MVuyN|k3OjV3OB?=
OV2008 NEW4W3hCeG:ydH;zbZMhSXO|YYm= MVmyOE81QCCq NFrLboZmdmijbnPld{BkcXOybHH0bY4ucW6mdXPl[EBieG:ydH;zbZM> MnvjNlM6PjJ3NUi=
W480  MVnGeY5kfGmxbjDBd5NigQ>? NVXuNZlMOi53L{GwJO69VQ>? M1LWd|MxKG2rbh?= M{K5WWROW09? NF7aNYV{\W6|aYTpfoV{KGOnbHzzJJRwKGOqZX3vdoFlcW:2aHXyZZB6KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NF3ZOZQzOzl|NEm3Ni=>
SW837 NGm0T2JHfW6ldHnvckBCe3OjeR?= NF7ySIwzNjVxMUCg{txO NIroO5A{OCCvaX6= MkL5SG1UVw>? MkTod4Vve2m2aYrld{Bk\WyuczD0c{BkcGWvb4Lh[IlwfGincnHwfUBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NVX4U4MxOjN7M{S5O|I>
T24 NWfo[GtyTnWwY4Tpc44hSXO|YYm= MkLXNk8yOC9{MDFOwG0> MUKyOEBp MUHjZZV{\XNiZH;z[U1l\XCnbnTlcpQhcW6qaXLpeIlwdiCxZjD0bIUhS1iFTEGyMYlv\HWlZXSgbY5kemWjc3Wgc4YhcW64YXTpcoch[2WubIO= M{HtVVI{PTJ4MEe5
CNE1 NEXoeXNHfW6ldHnvckBCe3OjeR?= MWCyNEDDvU1? NFjRNZk1QCCq M2HUW4Jtd2OtczD0bIUhUUxvNjDpcoNz\WG|ZXSgdIhwe3Cqb4L5cIF1cW:wIH;mJHN1[XR| NX76ems1OjN|OEK5NVQ>
CNE2 NHjoS5lHfW6ldHnvckBCe3OjeR?= NXPC[lRbOjBiwsXN MXq0PEBp M{DnN4Jtd2OtczD0bIUhUUxvNjDpcoNz\WG|ZXSgdIhwe3Cqb4L5cIF1cW:wIH;mJHN1[XR| M3j5XFI{Ozh{OUG0
HONE1 MUnGeY5kfGmxbjDBd5NigQ>? M3;2b|IxKML3TR?= NGLIfFk1QCCq M2TX[4Jtd2OtczD0bIUhUUxvNjDpcoNz\WG|ZXSgdIhwe3Cqb4L5cIF1cW:wIH;mJHN1[XR| M13xb|I{Ozh{OUG0
CNE1 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2\MRlQh|ryP NF\RZXN{cWewaX\pZ4FvfGy7IILl[JVk\XNiY3XscEB3cWGkaXzpeJk> Mn2zNlM{QDJ7MUS=
CNE1 Ml7MSpVv[3Srb36gRZN{[Xl? MmrnNE0zOCEQvF2= MYiwMVQhcA>? NVXYWJNkcW6qaXLpeJMhW3SjdEOgZYN1cX[jdHnvckBqdiCjIHTvd4UuKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> M1fYelI{Ozh{OUG0
CNE2 MXjGeY5kfGmxbjDBd5NigQ>? M{DXXFAuOjBizszN M{PSPVAuPCCq M3:zUIlvcGmkaYTzJHN1[XR|IHHjeIl3[XSrb36gbY4h[SCmb4PlMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy NVnyNFlFOjN|OEK5NVQ>
HONE1 NGf6VGJHfW6ldHnvckBCe3OjeR?= MX2wMVIxKM7:TR?= NEjDNZMxNTRiaB?= NGjSdZJqdmirYnn0d{BUfGG2MzDhZ5RqfmG2aX;uJIlvKGFiZH;z[U0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> Mmr2NlM{QDJ7MUS=
CNE1 MnTJR4VtdCCYaXHibYxqfHliQYPzZZk> MVOwMlUuPjRizszN NEnCWG01QCCq NFe0UFd{fXCycnXzd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> MYmyN|M5OjlzNB?=
CNE2 MmXER4VtdCCYaXHibYxqfHliQYPzZZk> MnrKNE42NTZ2IN88US=> MnnGOFghcA>? MUfzeZBxemW|c3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT2gZY5lKHSrbXWt[IVx\W6mZX70JI1idm6nch?= NWTTPFJ5OjN|OEK5NVQ>
HONE1 M2G2c2NmdGxiVnnhZoltcXS7IFHzd4F6 MViwMlUuPjRizszN NELCfnY1QCCq NHL2S|h{fXCycnXzd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> NGHJSpEzOzN6MkmxOC=>
C666-1  Ml7NR4VtdCCYaXHibYxqfHliQYPzZZk> NGm5UIExNjVvNkSg{txO Mk\tOFghcA>? MkD4d5VxeHKnc4Pld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3WtJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK= MU[yN|M5OjlzNB?=
CNE1 M2D1eWFxd3C2b4Ppd{BCe3OjeR?= NFnrW5oyOCEEtV5CpC=> MXO0PEBp MlrDbY5lfWOnczDhdI9xfG:|aYRCpC=> MUCyN|M5OjlzNB?=
CNE2 M4q4WWFxd3C2b4Ppd{BCe3OjeR?= NGfFbZIyOCEEtV5CpC=> MYC0PEBp NFrwUHdqdmS3Y3XzJIFxd3C2b4Ppd:Kh MWqyN|M5OjlzNB?=
HONE1 MV3BdI9xfG:|aYOgRZN{[Xl? Ml\VNVAhyrWPwrC= MmrGOFghcA>? MW\pcoR2[2W|IHHwc5B1d3Orc9Mg MkDrNlM{QDJ7MUS=
CNE2 MmTuR4VtdCCYaXHibYxqfHliQYPzZZk> MVWxM|Ih|ryP NULBb2ZpPDhiaB?= M3rIZpNmdnOrdHn6[UBk\WyuczD0c{Bz[WSrb4To[ZJieHl? M1zJXFI{Ozh{OUG0
HONE1 MlTMR4VtdCCYaXHibYxqfHliQYPzZZk> MkTHNU8zKM7:TR?= NX\VbpFKPDhiaB?= M3L6T5NmdnOrdHn6[UBk\WyuczD0c{Bz[WSrb4To[ZJieHl? MluxNlM{QDJ7MUS=
C666-1  NFHqe2RE\WyuIG\pZYJqdGm2eTDBd5NigQ>? Mm\TNU8zKM7:TR?= NWPwTYJyPDhiaB?= MmHGd4Vve2m2aYrlJINmdGy|IITvJJJi\GmxdHjldoFxgQ>? MVmyN|M5OjlzNB?=
HEC-1A M1zWNGZ2dmO2aX;uJGF{e2G7 NXL3b|I6OcLizszNxsA> NWTSbJZPOjRiaB?= M{i5UGROW09? M{nPUYJtd2OtczD0bIUhVVWFMkCt[Y5p[W6lZXSgbY53[XOrb36geJJq\2encnXkJIJ6KDFyJTDGRnM> M2T6eVI{OjZ{MkC4
RL95-2 NIrB[VlHfW6ldHnvckBCe3OjeR?= M1rBW|HDqM7:TdMg MYiyOEBp MmjPSG1UVw>? M{HsfoJtd2OtczD0bIUhVVWFMkCt[Y5p[W6lZXSgbY53[XOrb36geJJq\2encnXkJIJ6KDFyJTDGRnM> Mn\XNlMzPjJ{MEi=
HEC-1A NV72[ZNzTnWwY4Tpc44hSXO|YYm= MUixxsDPxE4EoB?= NXLnZlB2OjRiaB?= NUTHZo5RTE2VTx?= MmnaZoxw[2u|IITo[UBOXUN{MD3lcohidmOnZDDpcpZie2mxbjD0dolo\2W{ZXSgZpkhTUeIwrC= MlvZNlMzPjJ{MEi=
RL95-2 NXWyTpBVTnWwY4Tpc44hSXO|YYm= MofCNeKh|ryPwrC= MV6yOEBp NX64NVVyTE2VTx?= NV65UpRr[myxY3vzJJRp\SCPVVOyNE1mdmijbnPl[EBqdn[jc3nvckB1emmpZ3Xy[YQh[nliRVfGxsA> Mo\YNlMzPjJ{MEi=
CT26 NVG5T2R[TnWwY4Tpc44hSXO|YYm= MVuyNEBuVQ>? Mk\oNUBp M1:5R5N2eHC{ZYPz[ZMhUEeILXnu[JVk\WRiVlXHSkBmgHC{ZYPzbY9vyqB? Mke3NlMzOzNzNkO=
UM-SCC-17B NXv2TVk2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkL3TWM2OD1{LkW2NkDDuSByLkSwPUDPxE1uIFfJOVA:OS5{N{mgxtEhOC5zOUSg{txO M3\IelIzPzdyOEm5
OSC-19 M3f4XGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYjYe4pyUUN3ME2zMlQ5OSEEsTCwMlk2OyEQvF2sJGdKPTB;MT6zOlYhyrFiMD63O|Ah|ryP M{[4RVIzPzdyOEm5
Cal33 NHfTcFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTJwMkiyJOKyKDBwNEKzJO69VSxiR1m1NF0yNjN2OTFCtUAxNjN4MzFOwG0> MWOyNlc4ODh7OR?=
UM-SCC-22B NEfHO4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnznTWM2OD1{Lk[0PEDDuSByLkW0NkDPxE1uIFfJOVA:OS5|MkCgxtEhOC5{MESg{txO NGHjWmwzOjd5MEi5PS=>
UM-SCC-17B Mn21SpVv[3Srb36gRZN{[Xl? MnK5NE0{OCEQvF2= MUSwMVI1KGh? NWrQN|JUcW6qaXLpeJMhW1SDVEOgZYN1cX[jdHnvckBld3OnIHHu[EB1cW2nIHTldIVv\GWwdHz5 M1TvNlIzPzdyOEm5
OSC-19 NH7aVItHfW6ldHnvckBCe3OjeR?= MUiwMVMxKM7:TR?= M1;zOFAuOjRiaB?= NGHUZZVqdmirYnn0d{BUXEGWMzDhZ5RqfmG2aX;uJIRwe2ViYX7kJJRqdWViZHXw[Y5l\W62bIm= M13G[lIzPzdyOEm5
Cal33 M2P4UWZ2dmO2aX;uJGF{e2G7 MXqwMVMxKM7:TR?= NEXld2kxNTJ2IHi= M1LqcYlvcGmkaYTzJHNVSVR|IHHjeIl3[XSrb36g[I9{\SCjbnSgeIlu\SCmZYDlcoRmdnSueR?= M2DzPFIzPzdyOEm5
UM-SCC-22B NVP5U2hRTnWwY4Tpc44hSXO|YYm= NUnsfnl2OC1|MDFOwG0> MkDuNE0zPCCq NWnSeldHcW6qaXLpeJMhW1SDVEOgZYN1cX[jdHnvckBld3OnIHHu[EB1cW2nIHTldIVv\GWwdHz5 NH7mUpAzOjd5MEi5PS=>
U-87MG MYLD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MWqwMVExKM7:TR?= NWrGSVRRPzJiaB?= MlWxSG1UVw>? NF\sSoxqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 NU\vVmNYOjV2M{[2PFI>
U-373MG M1;uZWNmdGxiVnnhZoltcXS7IFHzd4F6 MnnLNE0yOCEQvF2= NVHUU5U3PzJiaB?= MUnEUXNQ M2iwUolvcGmkaYTzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? MnT5NlU1OzZ4OEK=
SH-SY5Y MYLD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M{jTS|AuOTBizszN MojWO|IhcA>? MmG1SG1UVw>? Mn3wbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? MXqyOVQ{PjZ6Mh?=
Tu-9648 MnXxR4VtdCCYaXHibYxqfHliQYPzZZk> NIrq[4kxNTFyIN88US=> M2LtUlczKGh? NF3nb4pFVVOR NIG4fFBqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 M2njUFI2PDN4Nkiy
Neuro-2a MonVR4VtdCCYaXHibYxqfHliQYPzZZk> MmLQNE0yOCEQvF2= NUPZVJZVPzJiaB?= MmDXSG1UVw>? MX3pcohq[mm2czDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 NXj6OoFUOjV2M{[2PFI>
PCNs M3rJVGNmdGxiVnnhZoltcXS7IFHzd4F6 NYrKfnp3OC1zMDFOwG0> MWK3NkBp M{DiRmROW09? M1e5OIlvcGmkaYTzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? Mn;4NlU1OzZ4OEK=
PGCs M{DjOGNmdGxiVnnhZoltcXS7IFHzd4F6 MmL5NE0yOCEQvF2= NXH1WJlVPzJiaB?= NVuyZVZYTE2VTx?= M3HIVolvcGmkaYTzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? M4jOPFI2PDN4Nkiy
RAW264.7 NIHhO4FHfW6ldHnvckBCe3OjeR?= NFnHd4MyOCEQvF2= NXmyVpFnOTJiaB?= NH[2bZBi[nKxZ3H0[ZMhfGinIH3SUmEh\XiycnXzd4lwdnNib3[gTmFMOixiU2TBWFEtKFOWQWSyMEBidmRiU2TBWFMhcW6mdXPl[EBjgSCGT16gZY5lKFRvMjD0c5hqdg>? MlK0NlI1PTR2M{G=
RAW264.7 M{jtVGFxd3C2b4Ppd{BCe3OjeR?= NFvId3A2NzFyIN88US=> MXG0OUBucW5? NVP5[Wlx\W6qYX7j[ZMhfG:6aX7zJIlv\HWlZXSgZZBweHSxc3nzJIFv\CCPTWCgcI9{ew>? MkPoNlI1PTR2M{G=
SW480 MlPyR4VtdCCYaXHibYxqfHliQYPzZZk> MoS5OU8yOC9{MDFOwG0> MnftO|IhcA>? MoLubY5pcWKrdIOgZ4VtdCC4aXHibYxqfHlib3[geIhmKEGORFirM2NFOTN|K9MgZ4VtdHN? M1LLeVIyQTByM{m3
HCT116 NX;nbYVqS2WubDDWbYFjcWyrdImgRZN{[Xl? NWjCV5BrPS9zMD:yNEDPxE1? M1HUOlczKGh? NXjUS5NMcW6qaXLpeJMh[2WubDD2bYFjcWyrdImgc4YhfGinIFHMSGgsN0OGMUOzL:Kh[2WubIO= NXTFc5NQOjF7MECzPVc>
DLD-1  MVXD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NHHvZ4g2NzFyL{KwJO69VQ>? MlvCO|IhcA>? MoHhbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHlib3[geIhmKEGORFirM2NFOTN|K9MgZ4VtdHN? NXHLWmVPOjF7MECzPVc>
SNU387  MV;D[YxtKF[rYXLpcIl1gSCDc4PhfS=> NUWxfXJOOjEEoN88UeKh NVzxS3lYOjRiaB?= NVzrNIU{emWmdXPld{Bk\WyuII\pZYJqdGm2eR?= MlvJNlE{OTF7N{W=
SNU398 M1vrVmNmdGxiVnnhZoltcXS7IFHzd4F6 MVuyNOKh|ryPwrC= MofXNlQhcA>? NXjWdWtUemWmdXPld{Bk\WyuII\pZYJqdGm2eR?= MkXVNlE{OTF7N{W=
HepG2 MXzD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NGjUdlIzOMLizszNxsA> MmT2NlQhcA>? NYfsbXBoemWmdXPld{Bk\WyuII\pZYJqdGm2eR?= MkD0NlE{OTF7N{W=
Huh-7 M{jxZmNmdGxiVnnhZoltcXS7IFHzd4F6 MWKyNOKh|ryPwrC= NVruTXBHOjRiaB?= M1LtXZJm\HWlZYOgZ4VtdCC4aXHibYxqfHl? M4[4c|IyOzFzOUe1
VSMC Mo\OS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUGzM|UwOTBizszN M3OwNlMxKG2rbh?= M3rFS2ROW09? M2LhUJBz\X[nboTzJHBFT0ZvIHHu[EB1cHKxbXLpck1u\WSrYYTl[EBXW02FIIDyc4xq\mW{YYTpc44hcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NFrhVWczODh2N{OwOi=>
MDA-MB-231 M2n0PGFxd3C2b4Ppd{BCe3OjeR?= M2DNcVExKM7:TR?= NVe4bVJJOjRiaB?= NVTUOWdkTE2VT9Mg NUP6VGxbcW6mdXPld{BieG:ydH;zbZM> NH;mTmQyPzFzNECwOS=>
MDA-MB-435S MojiRZBweHSxc3nzJGF{e2G7 M3XuNVExKM7:TR?= M4jtR|I1KGh? MmGzSG1UV8Li NUXtRnFzcW6mdXPld{BieG:ydH;zbZM> Mnq2NVcyOTRyMEW=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-STAT3 / STAT3 ; 

PubMed: 25261365     


(A) PC3M-1E8 cells were treated with different doses of Stattic for 4 h. (B) PC3M-1E8 cells were treated with 10 μM Stattic for different time points. 

Survivin / c-Myc / Bcl-xl ; 

PubMed: 25261365     


Western blot analysis of STAT3 downstream targets in PC3M-1E8 cells treated with 10 μM Stattic for 24 h. 

PARP / C-PARP / Caspase-3 / C-Caspse-3 ; 

PubMed: 23382914     


NPC cells were exposed to the indicated concentrations of Stattic for 48 h; apoptotic cells were measured by western blot analysis of cleaved PARP and cleaved caspase-3. Protein levels were quantified using ImageJ software. 

25261365 23382914
Immunofluorescence
p-STAT3 / STAT3 / Survivin ; 

PubMed: 25261365     


Cellular localization of pSTAT3 and survivin (green) was observed by confocal microscopy after immunofluorescent staining of PC3M-1E8 cancer cells treated with 10 μM Stattic.

25261365
Growth inhibition assay
Cell viability; 

PubMed: 23382914     


Stattic inhibits the cell viability of NPC cells in a time-dependent manner. CNE2 cells were treated with 8 µM Stattic for the indicated times, and cell viability was measured by the MTT assay. 

23382914
ELISA
BDNF; 

PubMed: 27456333     


BDNF secretion was analyzed using ELISA in A549 and H1299 cells treated with or without Stattic. Results are shown as the mean ± S.E. n = 3; *p < 0.05, vs Control group, Student’s t-test. 

27456333

Protocol

Kinase Assay:

[1]

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High-Throughput Screening and FluorescencePolarization Assays:

Screening is performed at approximately 30 °C. The specificity ofscreening hits is validated in analogous assays for binding of thetest compounds to the SH2 domains of STAT1, STAT5, and Lck. The final concentration of buffer components used for all FP assays is 10 mM HEPES (pH 7.5), 1 mM EDTA, 0.1% Nonidet P-40, 50 mM NaCl, and 10% DMSO. The absence of dithiothreitol is essential for inhibitory activity. The sequences of the peptides are: STAT3, 5-carboxyfluorescein-GY(PO3H2)LPQTV-NH2; STAT1, 5-carboxyfluorescein-GY(PO3H2)DKPHVL; STAT5, 5-carboxyfluorescein-GY(PO3H2)LVLDKW; and Lck, 5-carboxyfluorescein-GY(PO3H2)EEIP. For specificity analysis at 30 °C, proteins are used at 150 nM (STAT1, STAT3, and STAT5). For specificity analysis at 37 °C, proteins are used at 370 nM (STAT3) or 100 nM (Lck). Proteins are incubated with test compounds in Eppendorf tubes at the indicated temperatures for 60 min prior addition of therespective 5-carboxyfluorescein labeled peptides (final concentration: 10 nM).Before measurement at room temperature, the mixtures are allowed to equilibrate for at least 30 min. Test compounds are used at the indicated concentrations diluted from a 20× stock in DMSO. Binding curves and inhibition curves are fitted with SigmaPlot. All competition curves are repeated three times in independent experiment
Cell Research:

[2]

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  • Cell lines: Ly3 cells
  • Concentrations: ~2.5 μM
  • Incubation Time: 48 h
  • Method:

    MTS


    (Only for Reference)
Animal Research:

[2]

- Collapse
  • Animal Models: Ly3 parental NOD/SCID IL2R null xenograft
  • Dosages: 3.75 mg/kg every day
  • Administration: intra tumor
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 42 mg/mL (198.87 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O
For best results, use promptly after mixing.
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 211.19
Formula

C8H5NO4S

CAS No. 19983-44-9
Storage powder
in solvent
Synonyms N/A
Smiles [O-][N+](=O)C1=CC=C2C=C[S](=O)(=O)C2=C1

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    We need to dissolve the compound for i.p. injection, could you give me some suggestions?

  • Answer:

    S7024 Stattic can be dissolved in 2% DMSO/PEG 300/dd H2O at 8 mg/mL as a clear solution.

STAT Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID