Stattic

Catalog No.S7024

Stattic Chemical Structure

Molecular Weight(MW): 211.19

Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1.

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In DMSO USD 90 In stock
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Cited by 60 Publications

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Choose Selective STAT Inhibitors

Biological Activity

Description Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1.
Features Stattic is the first non-peptide small molecule with inhibitory activity against STAT3 SH2 domain regardless of the STAT3 phosphorylation state in vitro.
Targets
STAT3 [1]
(Cell-free assay)
5.1 μM
In vitro

Stattic inhibits binding of a phosphotyrosine-containing peptide derived from the gp130 receptor to the STAT3 SH2 domain in a strongly temperature-dependent manner. Stattic only has a very weak effect on binding of a tyrosinephosphorylated peptide to the SH2-domain of the tyrosine kinase Lck. And it doesn’t inhibit dimerization of two other dimeric transcription factors (c-Myc/Max and Jun/Jun). It also inhibits fluorescein-labeled phosphopeptides to the SH2 domains of STAT1 and STAT5b. Stattic selectively inhibits DNA binding of STAT3 homodimers at a concentration of 10 μM. Shown to inhibit cellular phosphorylation of STAT3 at Tyr705 with little effect towards STAT1 phosphorylation at Tyr701 (in HepG2 cells) or the phosphorylation of JAK1, JAK2, and c-Src (in MDA-MB-231 and MDA-MB-235S cells). Stattic increases the apoptotic rate of STAT3-dependent breast cancer cell lines. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H9c2 MVzGeY5kfGmxbjDBd5NigQ>? M3;Tc|Ex6oDLzszN NUPPdm5LPOLCiXi= M4i5cpJmfmW{c3XzJJRp\SCnZn\lZ5R{KG:oIFnMMVI4 MkPTNlY{Ozl4M{O=
NPC  Mmj4SpVv[3Srb36gRZN{[Xl? M3LKdFAuPy53INM1US=> MXjhZo9tcXOqZYOgSW1VNWyra3WgcY9t\WO3bHHyJIFtfGW{YYTpc45{NCCjbnSgZ4VtdCCvaXfyZZRqd25iYX7kJIlvfmG|aX;uJIlv\HWlZXSgZpkhWkuLUDDrco9kc2Sxd36= NWXQXGlXOjV7MUW0N|A>
HASMC M2\mdWZ2dmO2aX;uJGF{e2G7 NHzrR2wyNjJ3LUWg{txO NGrEeY8zOCCvaX6= M{HwOmROW00EoB?= NWXxUJI1cW6qaXLpeJMheC1qWTmtV3RCXC1zLEOsOUB{cWewYXzzxsA> NGrLbmwzPTh2OU[yNi=>
H9c2  MV3GeY5kfGmxbjDBd5NigQ>? NITVVIIzNzFyIN88US=> MV6yJIg> NHLXNGVFVVORwrC= MUfhZpJw\2G2ZYRCpJRp\SCleYTvdJJwfGWldHn2[UBm\m[nY4TzJI9nKEmOLUK3JIFo[Wmwc4SgV2g> NWHXUFQxOjV6MkC5NFc>
A431 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV:5bodwOsLizszN NGLQU|IzyqCq MoDsZoxw[2u|IFXHSk1z\X[ncoPl[EBl\WO{ZXHz[ZMhcW5iY3XscEB3cWGkaXzpeJk> NGLxfIIzPTd{MESzOS=>
A431 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVOyxsDPxE1? M1Sx[FLDqGh? MVzpcoNz\WG|ZYOgbY4h[XCxcITvd4l{KGmwZIXj[YQh[nlic3jpb49vcW5? MXqyOVczODR|NR?=
SiHa NFzWb5ZE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M2q3d|UuPzVibl2= NHfNSGszPMLiaNMg M1[zZ5Npd3e|IH3vdpBpd2yxZ4mgc4Yh[SC2eYDpZ4FtKGGyb4D0c5Rq[yClZXzsJIFv\CCmb4PlMYRmeGWwZHXueEBtd3O|IH;mJINmdGxidnnhZoltcXS7wrC= NVrXPHBHOjV3M{m2OFQ>
SiHa M{jJXmZ2dmO2aX;uJGF{e2G7 MUO1MVc2KG6P MnS5NlTDqGkEoB?= NH\DVZFz\WS3Y3XzJJRp\cLicHjvd5Bpd3K7bHH0bY9vKGG2IITo[UB1gXKxc3nu[UBz\XOrZIXlJFcxPcLi NX2zeoF5OjV3M{m2OFQ>
ECA109 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYCwMVIxKM7:TR?= MonqNlTDqGkEoB?= NVfkTnd5UUN3ME21MlUxKM7:TR?= NEDa[FkzPTR7MkS4NC=>
TE13 M{LGcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmPzNE0zOCEQvF2= NULmbXE2OjUEoHlCpC=> MXnJR|UxRTZwMUZCpO69VQ>? NWTHcFZlOjV2OUK0PFA>
KYSE150  MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXfhNlN4OC1{MDFOwG0> NGDoTVQzPMLiaNMg MlHsTWM2OD1zMj62OOKh|ryP NVPOcndmOjV2OUK0PFA>
ECA109 M4SzdGNtd26xZ3XubYMhW3W{dnn2ZYwhSXO|YYm= MWGwMlXDqM7:TR?= Mm\JNlTDqGkEoB?= MmPSd5VxeHKnc4Pld{B1cGViY3zvco9o\W6rYzDmc5Ju[XSrb36= NVX2[WFMOjV2OUK0PFA>
TE13 NEXSRndEdG:wb3flcolkKFO3co\peoFtKEG|c3H5 NWCzeGFkOC53wrFOwG0> NVrlcllbOjUEoHlCpC=> MVzzeZBxemW|c3XzJJRp\SClbH;uc4dmdmmlIH\vdo1ifGmxbh?= M3rWfVI2PDl{NEiw
KYSE150  MnHWR4xwdm:pZX7pZ{BUfXK4aY\hcEBCe3OjeR?= NVr3Z41TOC53wrFOwG0> NW\ESYJLOjUEoHlCpC=> Ml;nd5VxeHKnc4Pld{B1cGViY3zvco9o\W6rYzDmc5Ju[XSrb36= M2W0TVI2PDl{NEiw
ECA109 M4fY[GZ2dmO2aX;uJGF{e2G7 MWGwMlXDqM7:TR?= M{\2XFI1yqCqwrC= MYHlcohidmOnczDJVk1qdmS3Y3XkJIdmdmW{YYTpc44hd2ZiRGPCdy=> NVrXfVBVOjV2OUK0PFA>
PC3M-1E8 MlzDSpVv[3Srb36gRZN{[Xl? M1jZb|IvPS93L{GwJO69VQ>? NVT5NFJ4OC12IHi= NEnKeFJqdmirYnn0d{B1cGViU2TBWFMh[WO2aY\heIlwdiCrbjDhJIRwe2VvIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XMEoB?= MlzsNlUzPjF|NkW=
PC3M-1E8 NIXEb|RHfW6ldHnvckBCe3OjeR?= NU[ze3lQOTBizszN NXrZfo14OjRiaB?= NVjHXFhV\G:5boLl[5Vt[XSnczDCZ4wugExuIIP1dpZqfmmwIHHu[EBkNU27Yx?= NFzmc2QzPTJ4MUO2OS=>
PC3M-1E8 MUfGeY5kfGmxbjDBd5NigQ>? MmLwNVAh|ryP M1\UZVI1KGh? NE[xS45qdmirYnn0d{BKVC14IHnu[JVk\WRiU2TBWFMh[WO2aY\heIlwdiCjbnSgeIhmKEmOLU[tbY5lfWOnZDDTWGFVOyCjY4TpeoF1cW:w MlrkNlUzPjF|NkW=
PC3M-1E8 NXvtUo8yS2yxbn;n[Y5q[yCVdYL2bZZidCCDc4PhfS=> NGLwV2ozNjVxNT:xNEDPxE1? MoPobY5pcWKrdIRCpJRp\SClb3zvcpkh\m:{bXH0bY9vKHOrZ37p[olk[W62bIm= MnT3NlUzPjF|NkW=
MDA-MB-231 NGDUXlRHfW6ldHnvckBCe3OjeR?= NYjodlBtOjBizszN NHnMOngzKGh? NVeyOVh5\XiqaXLpeJMhW26jaXygZY5lKEVvY3HkbIVzcW5iZYjwdoV{e2mxbh?= NI[4UnEzPTF3M{O0PS=>
H9c2 M2W2NGZ2dmO2aX;uJGF{e2G7 MUWyNEDDvU1? MXKzNEBucW5? MX7EUXNQyqB? NITScJpi[m:uaYPo[ZMheHKxcH;mc4wucW6mdXPl[EBCU1RicHjvd5Bpd3K7bHH0bY9vKGG2IHLveIghe2W{NEezJIFv\CC2aIKzNFg> MoKzNlUyODVyNke=
HaCaT  NIDhWGRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUXiNGRrOTBiwsXN MnzINlAhdWmw MX3EUXNQyqB? M3nneoVvcGGwY3XzJJNwemGoZX7pZk0h[W6mIIP1col1cW6rYj3pcoR2[2WmIHfyc5d1cCCrbnjpZol1cW:w M2PqUVI2ODF|OUC3
Caki-1 NW\3NIpPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mm\GNVAhyrWP MXqyNEBucW5? NXzUbINGTE2VT9Mg Mojo[Y5p[W6lZYOgd49z[W[nbnniMUBidmRic4XubZRqdmmkLXnu[JVk\WRiZ4Lve5RpKGmwaHnibZRqd25? M4T5OVI2ODF|OUC3
HaCaT  MUTBdI9xfG:|aYOgRZN{[Xl? NUHnN2RWOTBiwsXN MXmyNEBucW5? NXHqZlI4TE2VT9Mg NWXKOpJJcW6lcnXhd4V{KHC{b4DvdpRqd26|IH;mJIFxd3C2b4TpZ{Bk\WyuczDkeYUhfG9idILlZZRu\W62IIfpeIghe2:{YX\lcoljKG:{IIP1col1cW6rYh?= MlThNlUxOTN7MEe=
FHL-primed hNSCs MUXD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NWDTWoNjOC5yMj21JO69VQ>? MV63NkBp NGfkV|Vt\WGmczD0c{B1cGVibH;zd{Bw\iClZXzsJJZq[WKrbHn0fUBifCCqaXfoJINwdmOnboTyZZRqd25? M2TJXFI1QTR3NEO0
ELL-primed hNSCs NUXTN3NES2WubDDWbYFjcWyrdImgRZN{[Xl? M{XvS|AvODJvNTFOwG0> MnLYO|IhcA>? MX3s[YFleyC2bzD0bIUhdG:|czDv[kBk\WyuII\pZYJqdGm2eTDheEBpcWeqIHPvcoNmdnS{YYTpc44> M2jUbVI1QTR3NEO0
SS MkixR4VtdCCYaXHibYxqfHliQYPzZZk> M4W1XFEuOTBizszN M2G1dFczKGh? MoHXSG1UVw>? MnLpZ4F2e2W|IHGg[I9{\S2mZYDlcoRmdnRiaX7obYJqfGmxbjDv[kB1cGVidnnhZoltcXS7wrC= M2WyPVI1PzV4MUGx
SeAx  M2DMPWNmdGxiVnnhZoltcXS7IFHzd4F6 M{DDSlEuOTBizszN NFnCSHA4OiCq MVnEUXNQ NUi5b5N5[2G3c3XzJIEh\G:|ZT3k[ZBmdmSnboSgbY5pcWKrdHnvckBw\iC2aHWgeoli[mmuaYT5xsA> MlzhNlQ4PTZzMUG=
HuT-78 NFjNXINE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M1HOflEuOTBizszN NXvFOGJwPzJiaB?= MXLEUXNQ MnTaZ4F2e2W|IHGg[I9{\S2mZYDlcoRmdnRiaX7obYJqfGmxbjDv[kB1cGVidnnhZoltcXS7wrC= MnXONlQ4PTZzMUG=
CD4+ NUjYd2V{SXCxcITvd4l{KEG|c3H5 Mk\FNVDjiIoQvH2= NWfMXVU5OjRiaB?= NUXwNpdwTE2VTx?= NX\zeWY6cW6mdXPld{BieG:ydH;zbZMhe3S{b37ncJk> M{fzXFI1PzV4MUGx
MCF-7 NGnheXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHfpPYMxNjR4OT2zMlc2KM7:TR?= NH;NdZk2KGR? Mn;jdoVlfWOnczDj[YxtKG63bXLldkB{cWewaX\pZ4FvfGy7 MonmNlQ4OjhyN{i=
MCF-7/LCC1 Mn2zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3LjSFAvPDZ7LUOuO|Uh|ryP M2HyS|Uh\A>? MWDy[YR2[2W|IHPlcIwhdnWvYnXyJJNq\26rZnnjZY51dHl? MkPrNlQ4OjhyN{i=
MCF-7/LCC9 NHzQU2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mki3NE41PjlvMz63OUDPxE1? MWC1JIQ> M{jjWZJm\HWlZYOgZ4VtdCCwdX3i[ZIhe2mpbnnmbYNidnSueR?= NXfUS29VOjR5MkiwO|g>
HaCaT NVPBVm1lT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFfDSVIyOCEEtV2= MWCyNEBucW5? M2SwcmROW00EoB?= Mkfw[Y5p[W6lZYOg[ZZmem:uaX31d{1qdmS3Y3XkJINmdGxiZ4Lve5RpKGmwaHnibZRqd25? MnTCNlQ1OjNzM{G=
HaCaT MV;BdI9xfG:|aYOgRZN{[Xl? NVPyOFhPOTBiwsXN M3LzeVIxKG2rbh?= NVv2c4VPTE2VT9Mg NHy5NHhmdmijbnPld{B1cGViYYDvdJRwfGmlIHXm[oVkfHNib3[g[ZZmem:uaX31dy=> M4rIPFI1PDJ|MUOx
MDA-MB-231 MmDRSpVv[3Srb36gRZN{[Xl? M13xUlExKML3TR?= NEfYTpczPCCq NFrmcG5FVVORwrC= MVjy[YR2[2W|IGCtV3RCXDNiZYjwdoV{e2mxbh?= MnjxNlQ{PzZ3OE[=
SUM-159 MlPvSpVv[3Srb36gRZN{[Xl? NUL5SVBDOTBiwsXN MVSyOEBp NWPFcpRvTE2VT9Mg M136TpJm\HWlZYOgVE1UXEGWMzDlfJBz\XO|aX;u MXyyOFM4PjV6Nh?=
SK-BR-3 Mny4SpVv[3Srb36gRZN{[Xl? NUPzOpc2OTBiwsXN NITocY8zPCCq NXXPfXN2TE2VT9Mg NWOxWlY1emWmdXPld{BRNVOWQWSzJIV5eHKnc4Ppc44> M1vx[|I1Ozd4NUi2
MCF7-HER2 Mmn2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn\jNE0yOCEQvF2= NVfY[GxJPDhiaB?= NF:0OGdFVVOR M3i0Oolv\HWlZYOgZ4VtdCCmZXH0bEBld3OnIHTldIVv\GWwdHz5 MYiyOFI6PzVyOB?=
MCF7-HER2 NFTKfXlHfW6ldHnvckBCe3OjeR?= Mn7EOUDPxE1? MUSyOEBp NX3SXYZKTE2VTx?= Ml3w[IlucW6rc3jld{BUd3hvMjygU4N1NTRuIHHu[EB{dHWpIHX4dJJme3Orb36= NH\RfpMzPDJ7N{WwPC=>
MCF7-HER2 MnnLSpVv[3Srb36gRZN{[Xl? NVuwdmVlPSEQvF2= MWWyOEBp NFXRUXpFVVOR NWHiXZhD\GWlcnXhd4V{KHSqZTDlfJBz\XO|aX;uJIxmfmWuczDv[kBGVVRibXHyb4Vzeyxidnnt[Y51cW5iYX7kJJNtfWd? NH3w[VUzPDJ7N{WwPC=>
MCF7-HER2 NVnDclF{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3TQNVUh|ryP MlXKNlQhcA>? MW\EUXNQ NICxeHFmdmijbnPld{Bk\WyuIHfyc5d1cCCrbnjpZol1cW:wIHPvcYJqdmWmIIfpeIghUGW{Y3XweIlv M1fyOFI1Ojl5NUC4
HMECs  NEHsZ21HfW6ldHnvckBCe3OjeR?= MVexNEDPxE1? MYiyJIg> NVu3cHVycW6qaXLpeJMhUU[QzsJCpI1m\GmjdHXkJJBpd3OyaH;yfYxifGmxbjDv[kBUXEGWMTygV3RCXDJiYX7kJHNVSVR| MYeyOFIyOTN{Nx?=
HTR8/SVneo NY[1UYpJTnWwY4Tpc44hSXO|YYm= NH3R[nMyyqEQvF5CpC=> NYPSeoVGOcLiaB?= Mojod5VxeHKnc4Pl[EBQW01vaX7keYNm\CCVVFHUN{BxcG:|cHjvdplt[XSrb36= MYqyOFA3ODJ2MR?=
HTR8/SVneo MknVSpVv[3Srb36gRZN{[Xl? MYGwMlUwOcLizszNxsA> M17FZVQ5KGh? MWTy[ZN1d3KnczD0bIUh\XiycnXzd4lwdiCxZjDFMYNi\GincnnuJJN2eHC{ZYPz[YQh[nliT2PN MUGyOFA3ODJ2MR?=
HTR8/SVneo M1\qb2Z2dmO2aX;uJGF{e2G7 MUCxxsDPxE4EoB?= NH\MWZM1QCCq MoGwd4lodmmoaXPhcpRtgSCrbnPy[YF{\XNibXnndoF1cW:wIHL5JG9UVcLi M4HYVFI1ODZyMkSx
C13* M1Lr[mFxd3C2b4Ppd{BCe3OjeR?= NUPTXIpoOC1zMDFOwG0> NVG4N4s6OjRxNEigbC=> Mnr0bY5lfWOnczDhdI9xfG:|aYOgbY4h[SCmb4PlJIFv\CC2aX3lJIRmeGWwZHXueEBu[W6wZYK= M3[3UVI{QTZ{NUW4
OV2008 MV\BdI9xfG:|aYOgRZN{[Xl? MXqwMVExKM7:TR?= NVjwSXhKOjRxNEigbC=> NWjoRlB1cW6mdXPld{BieG:ydH;zbZMhcW5iYTDkc5NmKGGwZDD0bY1mKGSncHXu[IVvfCCvYX7u[ZI> MVKyN|k3OjV3OB?=
C13* MUTBdI9xfG:|aYOgRZN{[Xl? M1zRT|I1NzR6IHi= NELCfopmdmijbnPld{BkcXOybHH0bY4ucW6mdXPl[EBieG:ydH;zbZM> MoDNNlM6PjJ3NUi=
OV2008 MnnCRZBweHSxc3nzJGF{e2G7 MW[yOE81QCCq NHrTeY9mdmijbnPld{BkcXOybHH0bY4ucW6mdXPl[EBieG:ydH;zbZM> M{HWTlI{QTZ{NUW4
W480  MX3GeY5kfGmxbjDBd5NigQ>? M{P2XFIvPS9zMDFOwG0> M1XoS|MxKG2rbh?= NHXzSZJFVVOR MWPz[Y5{cXSrenXzJINmdGy|IITvJINp\W2xcnHkbY91cGW{YYD5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NHzaV4YzOzl|NEm3Ni=>
SW837 NF36WZdHfW6ldHnvckBCe3OjeR?= NFfUcWYzNjVxMUCg{txO M3vWUVMxKG2rbh?= M{S0UmROW09? Mm[zd4Vve2m2aYrld{Bk\WyuczD0c{BkcGWvb4Lh[IlwfGincnHwfUBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MXiyN|k{PDl5Mh?=
T24 M3nDW2Z2dmO2aX;uJGF{e2G7 NWTo[4hHOi9zMD:yNEDPxE1? NWfHXG1sOjRiaB?= MX;jZZV{\XNiZH;z[U1l\XCnbnTlcpQhcW6qaXLpeIlwdiCxZjD0bIUhS1iFTEGyMYlv\HWlZXSgbY5kemWjc3Wgc4YhcW64YXTpcoch[2WubIO= MUKyN|UzPjB5OR?=
CNE1 NXXIOFhKTnWwY4Tpc44hSXO|YYm= NEXBSlEzOCEEtV2= MnPnOFghcA>? MXTicI9kc3NidHjlJGlNNTZiaX7jdoVie2WmIIDoc5NxcG:{eXzheIlwdiCxZjDTeIF1Ow>? M3i5PFI{Ozh{OUG0
CNE2 NXnm[ohoTnWwY4Tpc44hSXO|YYm= MYKyNEDDvU1? M1K5WVQ5KGh? NX\6U2dk[myxY3vzJJRp\SCLTD22JIlv[3KnYYPl[EBxcG:|cHjvdplt[XSrb36gc4YhW3SjdEO= MVKyN|M5OjlzNB?=
HONE1 NHTyZ3BHfW6ldHnvckBCe3OjeR?= NHHXcY8zOCEEtV2= MWC0PEBp MkjlZoxw[2u|IITo[UBKVC14IHnuZ5Jm[XOnZDDwbI9{eGixconsZZRqd25ib3[gV5RifDN? NVToXYZSOjN|OEK5NVQ>
CNE1 M3;RRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVO0JO69VQ>? M1HyUZNq\26rZnnjZY51dHlicnXkeYNmeyClZXzsJJZq[WKrbHn0fS=> MViyN|M5OjlzNB?=
CNE1 NXzud2RJTnWwY4Tpc44hSXO|YYm= NUPuVVdXOC1{MDFOwG0> NFfOUmoxNTRiaB?= MYLpcohq[mm2czDTeIF1OyCjY4TpeoF1cW:wIHnuJIEh\G:|ZT2gZY5lKHSrbXWt[IVx\W6mZX70JI1idm6nch?= MYCyN|M5OjlzNB?=
CNE2 NGHlR2FHfW6ldHnvckBCe3OjeR?= MofmNE0zOCEQvF2= NWGxfFg6OC12IHi= NVXBSFlZcW6qaXLpeJMhW3SjdEOgZYN1cX[jdHnvckBqdiCjIHTvd4UuKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> NUnZVJp3OjN|OEK5NVQ>
HONE1 MkHUSpVv[3Srb36gRZN{[Xl? MUewMVIxKM7:TR?= M{\BflAuPCCq NWrEVnFIcW6qaXLpeJMhW3SjdEOgZYN1cX[jdHnvckBqdiCjIHTvd4UuKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> NXfYXWxKOjN|OEK5NVQ>
CNE1 MVjD[YxtKF[rYXLpcIl1gSCDc4PhfS=> Mn\yNE42NTZ2IN88US=> MmLEOFghcA>? NEj5N3d{fXCycnXzd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> NFPV[G0zOzN6MkmxOC=>
CNE2 MWjD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NYm1ZlJmOC53LU[0JO69VQ>? M3nvb|Q5KGh? MmXpd5VxeHKnc4Pld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3WtJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK= MluzNlM{QDJ7MUS=
HONE1 M3;tbWNmdGxiVnnhZoltcXS7IFHzd4F6 Mn:5NE42NTZ2IN88US=> M3PCSVQ5KGh? M{P1[pN2eHC{ZYPz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy MYqyN|M5OjlzNB?=
C666-1  NGnWbHBE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MX[wMlUuPjRizszN NEO3SW81QCCq NHr4dnl{fXCycnXzd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> NVvXfJd5OjN|OEK5NVQ>
CNE1 NWXxRXc5SXCxcITvd4l{KEG|c3H5 NGDBN4UyOCEEtV5CpC=> M4ezSVQ5KGh? MXfpcoR2[2W|IHHwc5B1d3Orc9Mg MWGyN|M5OjlzNB?=
CNE2 NGfWd4pCeG:ydH;zbZMhSXO|YYm= NVTXboYxOTBiwsXNxsA> MnXuOFghcA>? M1zBNIlv\HWlZYOgZZBweHSxc3nzxsA> MlzGNlM{QDJ7MUS=
HONE1 NX;CfIVySXCxcITvd4l{KEG|c3H5 MVGxNEDDvU4EoB?= NHHScmM1QCCq NUHMV4V4cW6mdXPld{BieG:ydH;zbZPDqA>? NILVeXYzOzN6MkmxOC=>
CNE2 M37FdWNmdGxiVnnhZoltcXS7IFHzd4F6 MUCxM|Ih|ryP MWK0PEBp M2nrOJNmdnOrdHn6[UBk\WyuczD0c{Bz[WSrb4To[ZJieHl? MV6yN|M5OjlzNB?=
HONE1 MVrD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NHTJUlEyNzJizszN NFzPW5M1QCCq NW\DR41He2Wwc3n0bZpmKGOnbHzzJJRwKHKjZHnveIhmemGyeR?= MWGyN|M5OjlzNB?=
C666-1  NHnjfVFE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M1zDUlEwOiEQvF2= MlTjOFghcA>? Mn\Id4Vve2m2aYrlJINmdGy|IITvJJJi\GmxdHjldoFxgQ>? MlnRNlM{QDJ7MUS=
HEC-1A M1KxV2Z2dmO2aX;uJGF{e2G7 Moe2NeKh|ryPwrC= NUO4SpNwOjRiaB?= NXfMfXIxTE2VTx?= NUP1ZmNn[myxY3vzJJRp\SCPVVOyNE1mdmijbnPl[EBqdn[jc3nvckB1emmpZ3Xy[YQh[nliMUClJGZDWw>? MoHLNlMzPjJ{MEi=
RL95-2 NWfMNXJrTnWwY4Tpc44hSXO|YYm= M{HuZlHDqM7:TdMg Mn7TNlQhcA>? NF;nd3BFVVOR Mki3Zoxw[2u|IITo[UBOXUN{MD3lcohidmOnZDDpcpZie2mxbjD0dolo\2W{ZXSgZpkhOTBnIF\CVy=> NXnRO2pjOjN{NkKyNFg>
HEC-1A NYLlXnhuTnWwY4Tpc44hSXO|YYm= MXexxsDPxE4EoB?= M2i0VVI1KGh? MYPEUXNQ MWricI9kc3NidHjlJG1WSzJyLXXubIFv[2WmIHnueoF{cW:wIITybYdo\XKnZDDifUBGT0cEoB?= NI\ze5UzOzJ4MkKwPC=>
RL95-2 MWHGeY5kfGmxbjDBd5NigQ>? M4TpTVHDqM7:TdMg Mn;LNlQhcA>? MUnEUXNQ MUHicI9kc3NidHjlJG1WSzJyLXXubIFv[2WmIHnueoF{cW:wIITybYdo\XKnZDDifUBGT0cEoB?= MmjpNlMzPjJ{MEi=
CT26 NEXhSGRHfW6ldHnvckBCe3OjeR?= NYDTOZZHOjBibV2= MXOxJIg> M2G0[5N2eHC{ZYPz[ZMhUEeILXnu[JVk\WRiVlXHSkBmgHC{ZYPzbY9vyqB? M4T1NVI{OjN|MU[z
UM-SCC-17B M{C0N2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXvWOXR5UUN3ME2yMlU3OiEEsTCwMlQxQSEQvF2sJGdKPTB;MT6yO|khyrFiMD6xPVQh|ryP MViyNlc4ODh7OR?=
OSC-19 NF\C[mlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmLMTWM2OD1|LkS4NUDDuSByLkm1N{DPxE1uIFfJOVA:OS5|Nk[gxtEhOC55N{Cg{txO NULadldxOjJ5N{C4PVk>
Cal33 MnfvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnHkTWM2OD1{LkK4NkDDuSByLkSyN{DPxE1uIFfJOVA:OS5|NEmgxtEhOC5|NkOg{txO NFrDUpkzOjd5MEi5PS=>
UM-SCC-22B NVfUVWtZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4jtZmlEPTB;Mj62OFghyrFiMD61OFIh|ryPLDDHTVUxRTFwM{KwJOKyKDBwMkC0JO69VQ>? NYfRWll{OjJ5N{C4PVk>
UM-SCC-17B NEjRUWlHfW6ldHnvckBCe3OjeR?= M1fzb|AuOzBizszN NHjxc4cxNTJ2IHi= MWPpcohq[mm2czDTWGFVOyCjY4TpeoF1cW:wIHTvd4Uh[W6mIITpcYUh\GWyZX7k[Y51dHl? MmTENlI4PzB6OUm=
OSC-19 MXvGeY5kfGmxbjDBd5NigQ>? NUPUOoJTOC1|MDFOwG0> NXu1fo1MOC1{NDDo M4K1O4lvcGmkaYTzJHNVSVR|IHHjeIl3[XSrb36g[I9{\SCjbnSgeIlu\SCmZYDlcoRmdnSueR?= NE\RW5QzOjd5MEi5PS=>
Cal33 MofBSpVv[3Srb36gRZN{[Xl? NFLuWJIxNTNyIN88US=> NIPUe5QxNTJ2IHi= NGTUNpJqdmirYnn0d{BUXEGWMzDhZ5RqfmG2aX;uJIRwe2ViYX7kJJRqdWViZHXw[Y5l\W62bIm= M3rSS|IzPzdyOEm5
UM-SCC-22B MlG0SpVv[3Srb36gRZN{[Xl? MnPwNE0{OCEQvF2= MV6wMVI1KGh? NF7VTYZqdmirYnn0d{BUXEGWMzDhZ5RqfmG2aX;uJIRwe2ViYX7kJJRqdWViZHXw[Y5l\W62bIm= M3jUWVIzPzdyOEm5
U-87MG M2ThS2NmdGxiVnnhZoltcXS7IFHzd4F6 MVmwMVExKM7:TR?= M4HZc|czKGh? MnrSSG1UVw>? Mof6bY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? NV;PTlhLOjV2M{[2PFI>
U-373MG NHrRRXhE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MUewMVExKM7:TR?= NWTHZWlrPzJiaB?= MWLEUXNQ NVfUe5F1cW6qaXLpeJMh[2WubDD2bYFjcWyrdImg[I9{\SCmZYDlcoRmdnSueR?= MV6yOVQ{PjZ6Mh?=
SH-SY5Y MVrD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M2fYZ|AuOTBizszN NY[zSXk3PzJiaB?= NXPxV5VTTE2VTx?= MV\pcohq[mm2czDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 NHr3WmYzPTR|Nk[4Ni=>
Tu-9648 NIrBT5lE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MnzPNE0yOCEQvF2= NWTNc|lbPzJiaB?= MmPpSG1UVw>? MmLabY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? NV;DfHBpOjV2M{[2PFI>
Neuro-2a NXHkU2V3S2WubDDWbYFjcWyrdImgRZN{[Xl? NXq1VmRlOC1zMDFOwG0> MWi3NkBp NX6zTYZ1TE2VTx?= NEj5WJJqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 NIOyN5ozPTR|Nk[4Ni=>
PCNs NEjzVFhE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NYXVTHp[OC1zMDFOwG0> MmDtO|IhcA>? NE\IcHFFVVOR NI\LNnBqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 M{DDVVI2PDN4Nkiy
PGCs MoSyR4VtdCCYaXHibYxqfHliQYPzZZk> NV;afZd3OC1zMDFOwG0> NFviR2o4OiCq NHT0d4RFVVOR MYXpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 NVXU[5ZuOjV2M{[2PFI>
RAW264.7 Mn\PSpVv[3Srb36gRZN{[Xl? NH7DN4oyOCEQvF2= MmfqNVIhcA>? NXPCdoFW[WK{b3fheIV{KHSqZTDtVm5CKGW6cILld5Nqd26|IH;mJGpCUzJuIGPURXQyNCCVVFHUNkwh[W6mIGPURXQ{KGmwZIXj[YQh[nliRF;OJIFv\CCWLUKgeI95cW5? NHf0R5gzOjR3NESzNS=>
RAW264.7 NVrwc|JMSXCxcITvd4l{KEG|c3H5 Mlq3OU8yOCEQvF2= NVH3XHJjPDVibXnu M1zPeoVvcGGwY3XzJJRwgGmwczDpcoR2[2WmIHHwc5B1d3OrczDhcoQhVU2SIHzvd5M> NHrNPJUzOjR3NESzNS=>
SW480 NWr6coczS2WubDDWbYFjcWyrdImgRZN{[Xl? NVrZXFZQPS9zMD:yNEDPxE1? NUnhTFVQPzJiaB?= MkH5bY5pcWKrdIOgZ4VtdCC4aXHibYxqfHlib3[geIhmKEGORFirM2NFOTN|K9MgZ4VtdHN? NFP6SmgzOTlyMEO5Oy=>
HCT116 MnfLR4VtdCCYaXHibYxqfHliQYPzZZk> NUG1dWRIPS9zMD:yNEDPxE1? NFrG[nI4OiCq NXnOZ|ROcW6qaXLpeJMh[2WubDD2bYFjcWyrdImgc4YhfGinIFHMSGgsN0OGMUOzL:Kh[2WubIO= NIKxbHEzOTlyMEO5Oy=>
DLD-1  MXnD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M3TISFUwOTBxMkCg{txO M1rJ[|czKGh? M2[4dYlvcGmkaYTzJINmdGxidnnhZoltcXS7IH;mJJRp\SCDTFTIL{9ETDF|MzxCpINmdGy| NYP2T5FlOjF7MECzPVc>
SNU387  Mnj4R4VtdCCYaXHibYxqfHliQYPzZZk> MYOyNOKh|ryPwrC= NY\FfWkyOjRiaB?= NF3x[ZNz\WS3Y3XzJINmdGxidnnhZoltcXS7 NVmxZlU5OjF|MUG5O|U>
SNU398 NVrhUHE4S2WubDDWbYFjcWyrdImgRZN{[Xl? MlrzNlDDqM7:TdMg NX:4VVFTOjRiaB?= NYThUFVVemWmdXPld{Bk\WyuII\pZYJqdGm2eR?= NYTNflc{OjF|MUG5O|U>
HepG2 M1PmcGNmdGxiVnnhZoltcXS7IFHzd4F6 MWWyNOKh|ryPwrC= MXOyOEBp MUTy[YR2[2W|IHPlcIwhfmmjYnnsbZR6 NH3IO|YzOTNzMUm3OS=>
Huh-7 NFLBZ2lE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NHzNbIMzOMLizszNxsA> NEXueXAzPCCq NHfPeXhz\WS3Y3XzJINmdGxidnnhZoltcXS7 MVGyNVMyOTl5NR?=
VSMC M4\EeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVyzM|UwOTBizszN NYXDc|ZkOzBibXnu NEjTSJlFVVOR MoXTdJJmfmWwdIOgVGRITi1iYX7kJJRpem:vYnnuMY1m\GmjdHXkJHZUVUNicILvcIln\XKjdHnvckBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> M4LJeVIxQDR5M{C2
MDA-MB-231 M3nM[2Fxd3C2b4Ppd{BCe3OjeR?= MVOxNEDPxE1? NXjRVYlSOjRiaB?= NEX6PIpFVVORwrC= MYjpcoR2[2W|IHHwc5B1d3Orcx?= NYPXeZZJOTdzMUSwNFU>
MDA-MB-435S MXXBdI9xfG:|aYOgRZN{[Xl? NFzLcY0yOCEQvF2= MV6yOEBp MXTEUXNQyqB? MoeybY5lfWOnczDhdI9xfG:|aYO= MVqxO|EyPDByNR?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-STAT3 / STAT3 ; 

PubMed: 25261365     


(A) PC3M-1E8 cells were treated with different doses of Stattic for 4 h. (B) PC3M-1E8 cells were treated with 10 μM Stattic for different time points. 

Survivin / c-Myc / Bcl-xl ; 

PubMed: 25261365     


Western blot analysis of STAT3 downstream targets in PC3M-1E8 cells treated with 10 μM Stattic for 24 h. 

PARP / C-PARP / Caspase-3 / C-Caspse-3 ; 

PubMed: 23382914     


NPC cells were exposed to the indicated concentrations of Stattic for 48 h; apoptotic cells were measured by western blot analysis of cleaved PARP and cleaved caspase-3. Protein levels were quantified using ImageJ software. 

25261365 23382914
Immunofluorescence
p-STAT3 / STAT3 / Survivin ; 

PubMed: 25261365     


Cellular localization of pSTAT3 and survivin (green) was observed by confocal microscopy after immunofluorescent staining of PC3M-1E8 cancer cells treated with 10 μM Stattic.

25261365
Growth inhibition assay
Cell viability; 

PubMed: 23382914     


Stattic inhibits the cell viability of NPC cells in a time-dependent manner. CNE2 cells were treated with 8 µM Stattic for the indicated times, and cell viability was measured by the MTT assay. 

23382914
ELISA
BDNF; 

PubMed: 27456333     


BDNF secretion was analyzed using ELISA in A549 and H1299 cells treated with or without Stattic. Results are shown as the mean ± S.E. n = 3; *p < 0.05, vs Control group, Student’s t-test. 

27456333

Protocol

Kinase Assay:

[1]

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High-Throughput Screening and FluorescencePolarization Assays:

Screening is performed at approximately 30 °C. The specificity ofscreening hits is validated in analogous assays for binding of thetest compounds to the SH2 domains of STAT1, STAT5, and Lck. The final concentration of buffer components used for all FP assays is 10 mM HEPES (pH 7.5), 1 mM EDTA, 0.1% Nonidet P-40, 50 mM NaCl, and 10% DMSO. The absence of dithiothreitol is essential for inhibitory activity. The sequences of the peptides are: STAT3, 5-carboxyfluorescein-GY(PO3H2)LPQTV-NH2; STAT1, 5-carboxyfluorescein-GY(PO3H2)DKPHVL; STAT5, 5-carboxyfluorescein-GY(PO3H2)LVLDKW; and Lck, 5-carboxyfluorescein-GY(PO3H2)EEIP. For specificity analysis at 30 °C, proteins are used at 150 nM (STAT1, STAT3, and STAT5). For specificity analysis at 37 °C, proteins are used at 370 nM (STAT3) or 100 nM (Lck). Proteins are incubated with test compounds in Eppendorf tubes at the indicated temperatures for 60 min prior addition of therespective 5-carboxyfluorescein labeled peptides (final concentration: 10 nM).Before measurement at room temperature, the mixtures are allowed to equilibrate for at least 30 min. Test compounds are used at the indicated concentrations diluted from a 20× stock in DMSO. Binding curves and inhibition curves are fitted with SigmaPlot. All competition curves are repeated three times in independent experiment
Cell Research:

[2]

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  • Cell lines: Ly3 cells
  • Concentrations: ~2.5 μM
  • Incubation Time: 48 h
  • Method:

    MTS


    (Only for Reference)
Animal Research:

[2]

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  • Animal Models: Ly3 parental NOD/SCID IL2R null xenograft
  • Formulation: --
  • Dosages: 3.75 mg/kg every day
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 42 mg/mL (198.87 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+H2O
For best results, use promptly after mixing.
8mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 211.19
Formula

C8H5NO4S

CAS No. 19983-44-9
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    We need to dissolve the compound for i.p. injection, could you give me some suggestions?

  • Answer:

    S7024 Stattic can be dissolved in 2% DMSO/PEG 300/dd H2O at 8 mg/mL as a clear solution.

STAT Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID