Stattic

For research use only.

Catalog No.S7024

102 publications

Stattic Chemical Structure

Molecular Weight(MW): 211.19

Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1. Stattic induces apoptosis.

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Selleck's Stattic has been cited by 102 publications

Purity & Quality Control

Choose Selective STAT Inhibitors

Biological Activity

Description Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1. Stattic induces apoptosis.
Features Stattic is the first non-peptide small molecule with inhibitory activity against STAT3 SH2 domain regardless of the STAT3 phosphorylation state in vitro.
Targets
STAT3 [1]
(Cell-free assay)
5.1 μM
In vitro

Stattic inhibits binding of a phosphotyrosine-containing peptide derived from the gp130 receptor to the STAT3 SH2 domain in a strongly temperature-dependent manner. Stattic only has a very weak effect on binding of a tyrosinephosphorylated peptide to the SH2-domain of the tyrosine kinase Lck. And it doesn’t inhibit dimerization of two other dimeric transcription factors (c-Myc/Max and Jun/Jun). It also inhibits fluorescein-labeled phosphopeptides to the SH2 domains of STAT1 and STAT5b. Stattic selectively inhibits DNA binding of STAT3 homodimers at a concentration of 10 μM. Shown to inhibit cellular phosphorylation of STAT3 at Tyr705 with little effect towards STAT1 phosphorylation at Tyr701 (in HepG2 cells) or the phosphorylation of JAK1, JAK2, and c-Src (in MDA-MB-231 and MDA-MB-235S cells). Stattic increases the apoptotic rate of STAT3-dependent breast cancer cell lines. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H9c2 M2LRVWZ2dmO2aX;uJGF{e2G7 MWexNQKBkc7:TR?= MVS05qCKcA>? M{HCNJJmfmW{c3XzJJRp\SCnZn\lZ5R{KG:oIFnMMVI4 MXKyOlM{QTZ|Mx?=
NPC  MkP0SpVv[3Srb36gRZN{[Xl? NUDEdFVDOC15LkWgxtVO NV;FcGN2[WKxbHnzbIV{KEWPVD3sbYtmKG2xbHXjeYxieiCjbITldoF1cW:wczygZY5lKGOnbHygcYloemG2aX;uJIFv\CCrbo\hd4lwdiCrbnT1Z4VlKGK7IGLLTXAhc26xY3vkc5dv MmTTNlU6OTV2M{C=
HASMC M3LMV2Z2dmO2aX;uJGF{e2G7 M1L3dFEvOjVvNTFOwG0> Ml\oNlAhdWmw M37RVGROW00EoB?= MXzpcohq[mm2czDwMUh[MS2VVFHUMVEtOyx3IIPp[45idHQEoB?= MkezNlU5PDl4MkK=
H9c2  M1u5OWZ2dmO2aX;uJGF{e2G7 NFO5SWMzNzFyIN88US=> M4\qU|IhcA>? MYfEUXNQyqB? M4TQbYFjem:pYYTld:KhfGinIHP5eI9xem:2ZXP0bZZmKGWoZnXjeJMhd2ZiSVytNlch[WejaX7zeEBUUA>? NYW5ZXZROjV6MkC5NFc>
A431 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M17TT|LDqM7:TR?= NUPEc3ByOsLiaB?= MkPhZoxw[2u|IFXHSk1z\X[ncoPl[EBl\WO{ZXHz[ZMhcW5iY3XscEB3cWGkaXzpeJk> MnfjNlU4OjB2M{W=
A431 NXH5fZc{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWG4W4ZIOsLizszN MkDMNuKhcA>? NGLl[XJqdmO{ZXHz[ZMhcW5iYYDvdJRwe2m|IHnu[JVk\WRiYomgd4hqc2:waX6= MXyyOVczODR|NR?=
SiHa M1fyOWNmdGxiVnnhZoltcXS7IFHzd4F6 Mn7yOU04PSCwTR?= MoLrNlTDqGkEoB?= NXewV2xXe2ixd4OgcY9zeGixbH;nfUBw\iCjIIT5dIlk[WxiYYDvdJRwfGmlIHPlcIwh[W6mIHTvd4Uu\GWyZX7k[Y51KGyxc4Ogc4Yh[2WubDD2bYFjcWyrdIpCpC=> NGDBUIszPTV|OU[0OC=>
SiHa MX3GeY5kfGmxbjDBd5NigQ>? MnrmOU04PSCwTR?= NVTPe3VPOjUEoHlCpC=> MnTqdoVlfWOnczD0bIXDqHCqb4PwbI9zgWyjdHnvckBifCC2aHWgeJlzd3OrbnWgdoV{cWS3ZTC3NFXDqA>? MWmyOVU{QTZ2NB?=
ECA109 NFe3NYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWCwMVIxKM7:TR?= NGnGOZUzPMLiaNMg M3P4NGlEPTB;NT61NEDPxE1? NYi5[VdqOjV2OUK0PFA>
TE13 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVXNRXc3OC1{MDFOwG0> NILtelgzPMLiaNMg MnroTWM2OD14LkG1xsDPxE1? NIXac5czPTR7MkS4NC=>
KYSE150  M37PU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFe4PYoxNTJyIN88US=> NVvXR4ZsOjUEoHlCpC=> MoXQTWM2OD1zMj62OOKh|ryP MYCyOVQ6OjR6MB?=
ECA109 NWPnNFV1S2yxbn;n[Y5q[yCVdYL2bZZidCCDc4PhfS=> NWXWNldIOC53wrFOwG0> MoDyNlTDqGkEoB?= NG\MOlZ{fXCycnXzd4V{KHSqZTDjcI9vd2enbnnjJIZwem2jdHnvci=> M{TnWlI2PDl{NEiw
TE13 M3\QdmNtd26xZ3XubYMhW3W{dnn2ZYwhSXO|YYm= NEP2bVMxNjYEoN88US=> MV6yOOKhcMLi MUjzeZBxemW|c3XzJJRp\SClbH;uc4dmdmmlIH\vdo1ifGmxbh?= NVLZdJhqOjV2OUK0PFA>
KYSE150  NF3yVWxEdG:wb3flcolkKFO3co\peoFtKEG|c3H5 MV[wMlXDqM7:TR?= NY\rZ4FZOjUEoHlCpC=> MVzzeZBxemW|c3XzJJRp\SClbH;uc4dmdmmlIH\vdo1ifGmxbh?= NUDGRZVrOjV2OUK0PFA>
ECA109 M376PGZ2dmO2aX;uJGF{e2G7 Mon4NE42yqEQvF2= MoK4NlTDqGkEoB?= M2j0RoVvcGGwY3XzJGlTNWmwZIXj[YQh\2WwZYLheIlwdiCxZjDEV2J{ NFLhNIczPTR7MkS4NC=>
PC3M-1E8 MXnGeY5kfGmxbjDBd5NigQ>? M4exb|IvPS93L{GwJO69VQ>? Mm\YNE01KGh? NFHnWphqdmirYnn0d{B1cGViU2TBWFMh[WO2aY\heIlwdiCrbjDhJIRwe2VvIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XMEoB?= NEHldZczPTJ4MUO2OS=>
PC3M-1E8 M3zYO2Z2dmO2aX;uJGF{e2G7 NUO0VoZjOTBizszN NH3pSmgzPCCq MYjkc5dvemWpdXzheIV{KEKlbD34UEwhe3W{dnn2bY4h[W6mIHOtUZlk NXTvOXR2OjV{NkGzOlU>
PC3M-1E8 MXPGeY5kfGmxbjDBd5NigQ>? MXmxNEDPxE1? NH;RcWQzPCCq NX[zVlBjcW6qaXLpeJMhUUxvNjDpcoR2[2WmIGPURXQ{KGGldHn2ZZRqd25iYX7kJJRp\SCLTD22MYlv\HWlZXSgV3RCXDNiYXP0bZZifGmxbh?= MWSyOVI3OTN4NR?=
PC3M-1E8 NHrDe4VEdG:wb3flcolkKFO3co\peoFtKEG|c3H5 NFXlV28zNjVxNT:xNEDPxE1? MmPrbY5pcWKrdIRCpJRp\SClb3zvcpkh\m:{bXH0bY9vKHOrZ37p[olk[W62bIm= M{D2dlI2OjZzM{[1
MDA-MB-231 MWHGeY5kfGmxbjDBd5NigQ>? NWjkU2s3OjBizszN M1:2bFIhcA>? NVv3dYNs\XiqaXLpeJMhW26jaXygZY5lKEVvY3HkbIVzcW5iZYjwdoV{e2mxbh?= MWqyOVE2OzN2OR?=
H9c2 NXW4XpF6TnWwY4Tpc44hSXO|YYm= NF7rRYozOCEEtV2= M2PMNFMxKG2rbh?= MkDZSG1UV8Li M{Hsd4Fjd2yrc3jld{Bxem:yb3\vcE1qdmS3Y3XkJGFMXCCyaH;zdIhwenmuYYTpc44h[XRiYn;0bEB{\XJ2N{OgZY5lKHSqckOwPC=> NVnFXHd[OjVzMEWwOlc>
HaCaT  MnzjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1:4PFExKML3TR?= MX:yNEBucW5? M4rUPWROW00EoB?= NVfn[Hcz\W6qYX7j[ZMhe2:{YX\lcoljNSCjbnSgd5VvcXSrbnniMYlv\HWlZXSg[5Jwf3SqIHnubIljcXSrb36= Mmj4NlUxOTN7MEe=
Caki-1 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVqxNEDDvU1? M1;zVFIxKG2rbh?= MVPEUXNQyqB? MnzL[Y5p[W6lZYOgd49z[W[nbnniMUBidmRic4XubZRqdmmkLXnu[JVk\WRiZ4Lve5RpKGmwaHnibZRqd25? NHvXSXYzPTBzM{mwOy=>
HaCaT  NUK2d441SXCxcITvd4l{KEG|c3H5 MlS0NVAhyrWP NEXQeI4zOCCvaX6= NX\YfJo3TE2VT9Mg MoPobY5kemWjc3XzJJBzd3CxcoTpc45{KG:oIHHwc5B1d3SrYzDj[YxteyCmdXWgeI8hfHKnYYTt[Y51KHerdHigd49z[W[nbnniJI9zKHO3bnn0bY5q[g>? M2jUd|I2ODF|OUC3
FHL-primed hNSCs NHP5dllE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MknWNE4xOi13IN88US=> NXLabJd2PzJiaB?= NX;3eFFzdGWjZIOgeI8hfGinIHzvd5Mhd2ZiY3XscEB3cWGkaXzpeJkh[XRiaHnnbEBkd26lZX70doF1cW:w NXvkRY5pOjR7NEW0N|Q>
ELL-primed hNSCs NXvj[25HS2WubDDWbYFjcWyrdImgRZN{[Xl? NVnZZWlZOC5yMj21JO69VQ>? MXq3NkBp NEnoeHRt\WGmczD0c{B1cGVibH;zd{Bw\iClZXzsJJZq[WKrbHn0fUBifCCqaXfoJINwdmOnboTyZZRqd25? MknGNlQ6PDV2M{S=
SS MXTD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NULzdFBHOS1zMDFOwG0> M37peVczKGh? NYTGT4piTE2VTx?= M1HtXoNifXOnczDhJIRwe2VvZHXw[Y5l\W62IHnubIljcXSrb36gc4YhfGinII\pZYJqdGm2edMg NFu5XlIzPDd3NkGxNS=>
SeAx  M{[4fWNmdGxiVnnhZoltcXS7IFHzd4F6 NY\RRllTOS1zMDFOwG0> MW[3NkBp M2PFfGROW09? NFK1U2tk[XW|ZYOgZUBld3OnLXTldIVv\GWwdDDpcohq[mm2aX;uJI9nKHSqZTD2bYFjcWyrdIpCpC=> MVeyOFc2PjFzMR?=
HuT-78 NVLKe49tS2WubDDWbYFjcWyrdImgRZN{[Xl? NIi2e2YyNTFyIN88US=> NFf2WJQ4OiCq NFPkenVFVVOR M3z2foNifXOnczDhJIRwe2VvZHXw[Y5l\W62IHnubIljcXSrb36gc4YhfGinII\pZYJqdGm2edMg M4XCOFI1PzV4MUGx
CD4+ NIPxbZpCeG:ydH;zbZMhSXO|YYm= NWXKNIdNOTEkgJpOwI0> MmLmNlQhcA>? Mk[1SG1UVw>? NXHaSJdNcW6mdXPld{BieG:ydH;zbZMhe3S{b37ncJk> NEjnR2czPDd3NkGxNS=>
MCF-7 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnnMNE41PjlvMz63OUDPxE1? MoT0OUBl NUL4c4hDemWmdXPld{Bk\WyuIH71cYJmeiC|aXfubYZq[2GwdHz5 NGm0d2gzPDd{OEC3PC=>
MCF-7/LCC1 NFTqdldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmrlNE41PjlvMz63OUDPxE1? MX[1JIQ> M2j3[5Jm\HWlZYOgZ4VtdCCwdX3i[ZIhe2mpbnnmbYNidnSueR?= NYXEeGFCOjR5MkiwO|g>
MCF-7/LCC9 NWjMSZpCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXSwMlQ3QS1|Lke1JO69VQ>? NYDWZml{PSCm MnH5doVlfWOnczDj[YxtKG63bXLldkB{cWewaX\pZ4FvfGy7 NHPLXJQzPDd{OEC3PC=>
HaCaT M2fZdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHHxRYMyOCEEtV2= Mny4NlAhdWmw MVfEUXNQyqB? M4TDWYVvcGGwY3XzJIV3\XKxbHnteZMucW6mdXPl[EBk\WyuIHfyc5d1cCCrbnjpZol1cW:w NVrObpd[OjR2MkOxN|E>
HaCaT NIHRSppCeG:ydH;zbZMhSXO|YYm= M1n5XlExKML3TR?= NFfmN|gzOCCvaX6= NXTnNYpPTE2VT9Mg MlrE[Y5p[W6lZYOgeIhmKGGyb4D0c5Rq[yCnZn\lZ5R{KG:oIHX2[ZJwdGmvdYO= MVKyOFQzOzF|MR?=
MDA-MB-231 NW\wOJNDTnWwY4Tpc44hSXO|YYm= Mn7iNVAhyrWP NV3seI5iOjRiaB?= NEH5N25FVVORwrC= NGjKe2Jz\WS3Y3XzJHAuW1SDVEOg[ZhxemW|c3nvci=> NXTpNnFYOjR|N{[1PFY>
SUM-159 MnjQSpVv[3Srb36gRZN{[Xl? NGfWclUyOCEEtV2= NFX4SGczPCCq M{DyZWROW00EoB?= MYry[YR2[2W|IGCtV3RCXDNiZYjwdoV{e2mxbh?= MoP1NlQ{PzZ3OE[=
SK-BR-3 M2XFe2Z2dmO2aX;uJGF{e2G7 MoHLNVAhyrWP NHPwTJczPCCq Ml;LSG1UV8Li NWDmRWVRemWmdXPld{BRNVOWQWSzJIV5eHKnc4Ppc44> M1jwNlI1Ozd4NUi2
MCF7-HER2 MojLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIfSZWYxNTFyIN88US=> MUi0PEBp MX;EUXNQ NFLTNJJqdmS3Y3XzJINmdGxiZHXheIgh\G:|ZTDk[ZBmdmSnboTsfS=> NVnmOYtyOjR{OUe1NFg>
MCF7-HER2 M4HEPWZ2dmO2aX;uJGF{e2G7 MnPoOUDPxE1? NWDuW5BVOjRiaB?= NGPn[nlFVVOR MYHkbY1qdmm|aHXzJHNwgC1{LDDPZ5QuPCxiYX7kJJNtfWdiZYjwdoV{e2mxbh?= NU[xPHJiOjR{OUe1NFg>
MCF7-HER2 NXnGT3d5TnWwY4Tpc44hSXO|YYm= NYnKfIF{PSEQvF2= NYmwbYtIOjRiaB?= MXXEUXNQ Mnfu[IVkemWjc3XzJJRp\SCneIDy[ZN{cW:wIHzleoVteyCxZjDFUXQhdWG{a3Xyd{whfmmvZX70bY4h[W6mIIPseYc> NXnNdXR5OjR{OUe1NFg>
MCF7-HER2 NVfpZ|lIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3;4UVUh|ryP MWeyOEBp Mo\0SG1UVw>? M3;meIVvcGGwY3XzJINmdGxiZ4Lve5RpKGmwaHnibZRqd25iY3;tZolv\WRid3n0bEBJ\XKlZYD0bY4> MXGyOFI6PzVyOB?=
HMECs  NFTmfJRHfW6ldHnvckBCe3OjeR?= NYO0OZJLOTBizszN MV2yJIg> M2DMeYlvcGmkaYTzJGlHVs7zwrDt[YRq[XSnZDDwbI9{eGixconsZZRqd25ib3[gV3RCXDFuIGPURXQzKGGwZDDTWGFVOw>? MnfBNlQzOTF|Mke=
HTR8/SVneo MWnGeY5kfGmxbjDBd5NigQ>? NIDBW|IyyqEQvF5CpC=> MVyxxsBp M2LOb5N2eHC{ZYPz[YQhV1OPLXnu[JVk\WRiU2TBWFMheGixc4Doc5J6dGG2aX;u MmX5NlQxPjB{NEG=
HTR8/SVneo NUjiZVEzTnWwY4Tpc44hSXO|YYm= NGHLcWgxNjVxMdMg{txOyqB? Ml;kOFghcA>? Mmm3doV{fG:{ZYOgeIhmKGW6cILld5Nqd25ib3[gSU1k[WSqZYLpckB{fXCycnXzd4VlKGK7IF;TUS=> NGS2[48zPDB4MEK0NS=>
HTR8/SVneo MU\GeY5kfGmxbjDBd5NigQ>? M4PHNFHDqM7:TdMg NIPSZmE1QCCq NW\3TnJte2mpbnnmbYNidnSueTDpcoNz\WG|ZYOgcYloemG2aX;uJIJ6KE:VTdMg MlHpNlQxPjB{NEG=
C13* MUXBdI9xfG:|aYOgRZN{[Xl? M1LPbFAuOTBizszN NIDDW4gzPC92ODDo MkPhbY5lfWOnczDhdI9xfG:|aYOgbY4h[SCmb4PlJIFv\CC2aX3lJIRmeGWwZHXueEBu[W6wZYK= MXqyN|k3OjV3OB?=
OV2008 MlzjRZBweHSxc3nzJGF{e2G7 MnnGNE0yOCEQvF2= NU\KNYtiOjRxNEigbC=> NH3V[VNqdmS3Y3XzJIFxd3C2b4Ppd{BqdiCjIHTvd4Uh[W6mIITpcYUh\GWyZX7k[Y51KG2jbn7ldi=> NYD5dpBSOjN7NkK1OVg>
C13* M3;uOmFxd3C2b4Ppd{BCe3OjeR?= NUnnTJczOjRxNEigbC=> Mmrh[Y5p[W6lZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZZBweHSxc3nz M3zRVlI{QTZ{NUW4
OV2008 NIrmbm1CeG:ydH;zbZMhSXO|YYm= Mmn1NlQwPDhiaB?= NWGxb5BK\W6qYX7j[ZMh[2m|cHzheIlvNWmwZIXj[YQh[XCxcITvd4l{ NH;3RmMzOzl4MkW1PC=>
W480  MkCwSpVv[3Srb36gRZN{[Xl? NFHuZmEzNjVxMUCg{txO NELSPHY{OCCvaX6= NXLqeZJVTE2VTx?= MkDEd4Vve2m2aYrld{Bk\WyuczD0c{BkcGWvb4Lh[IlwfGincnHwfUBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NH:5VW8zOzl|NEm3Ni=>
SW837 M4n3PWZ2dmO2aX;uJGF{e2G7 M2WxclIvPS9zMDFOwG0> M373PFMxKG2rbh?= NIS3N|ZFVVOR NELqUGd{\W6|aYTpfoV{KGOnbHzzJJRwKGOqZX3vdoFlcW:2aHXyZZB6KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NG\mZ28zOzl|NEm3Ni=>
T24 NFyze5NHfW6ldHnvckBCe3OjeR?= MnTNNk8yOC9{MDFOwG0> MWOyOEBp NHT3bplk[XW|ZYOg[I9{\S2mZYDlcoRmdnRiaX7obYJqfGmxbjDv[kB1cGViQ2jDUFEzNWmwZIXj[YQhcW6lcnXhd4Uhd2ZiaX72ZYRqdmdiY3XscJM> NIPEToQzOzV{NkC3PS=>
CNE1 MWDGeY5kfGmxbjDBd5NigQ>? NEnQdm8zOCEEtV2= MlPnOFghcA>? NHzJOWNjdG:la4OgeIhmKEmOLU[gbY5kemWjc3XkJJBpd3OyaH;yfYxifGmxbjDv[kBUfGG2Mx?= Mo\wNlM{QDJ7MUS=
CNE2 MnK2SpVv[3Srb36gRZN{[Xl? NWTMPFZoOjBiwsXN MnHGOFghcA>? NVHKUWlw[myxY3vzJJRp\SCLTD22JIlv[3KnYYPl[EBxcG:|cHjvdplt[XSrb36gc4YhW3SjdEO= NXvqbXA2OjN|OEK5NVQ>
HONE1 NETwOpJHfW6ldHnvckBCe3OjeR?= NV7sVlZmOjBiwsXN M1v2dlQ5KGh? MYDicI9kc3NidHjlJGlNNTZiaX7jdoVie2WmIIDoc5NxcG:{eXzheIlwdiCxZjDTeIF1Ow>? MlXCNlM{QDJ7MUS=
CNE1 NXq2XZYzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NID5[WU1KM7:TR?= NIT2SFV{cWewaX\pZ4FvfGy7IILl[JVk\XNiY3XscEB3cWGkaXzpeJk> NWO1PVdkOjN|OEK5NVQ>
CNE1 NIj4V5NHfW6ldHnvckBCe3OjeR?= Mnf0NE0zOCEQvF2= M13xPVAuPCCq Mmi5bY5pcWKrdIOgV5RifDNiYXP0bZZifGmxbjDpckBiKGSxc3WtJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK= M3Pq[lI{Ozh{OUG0
CNE2 NF7sNmhHfW6ldHnvckBCe3OjeR?= MkjMNE0zOCEQvF2= NFX0XXcxNTRiaB?= NXjxNmh[cW6qaXLpeJMhW3SjdEOgZYN1cX[jdHnvckBqdiCjIHTvd4UuKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> M4HyNlI{Ozh{OUG0
HONE1 M4DGbWZ2dmO2aX;uJGF{e2G7 NVHNcIwxOC1{MDFOwG0> NYrtSXY1OC12IHi= NELldHVqdmirYnn0d{BUfGG2MzDhZ5RqfmG2aX;uJIlvKGFiZH;z[U0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> NUmxSnBsOjN|OEK5NVQ>
CNE1 MWnD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NIDqN44xNjVvNkSg{txO M2fPRVQ5KGh? NIDu[I1{fXCycnXzd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> NVy5W4VDOjN|OEK5NVQ>
CNE2 NHfzNWpE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NFLWV|IxNjVvNkSg{txO NFLQOWw1QCCq NH:4VVR{fXCycnXzd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> MoWwNlM{QDJ7MUS=
HONE1 MorQR4VtdCCYaXHibYxqfHliQYPzZZk> NFzQ[24xNjVvNkSg{txO NX\sNG9OPDhiaB?= MX7zeZBxemW|c3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT2gZY5lKHSrbXWt[IVx\W6mZX70JI1idm6nch?= NWHTbolSOjN|OEK5NVQ>
C666-1  NEHRPZpE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MYKwMlUuPjRizszN M3fFZlQ5KGh? M3\FXJN2eHC{ZYPz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy Mlv1NlM{QDJ7MUS=
CNE1 M4DEeWFxd3C2b4Ppd{BCe3OjeR?= M3qwd|ExKML3TdMg NGO1PGM1QCCq NHjHUWpqdmS3Y3XzJIFxd3C2b4Ppd:Kh NX35WXUxOjN|OEK5NVQ>
CNE2 NETQbJpCeG:ydH;zbZMhSXO|YYm= NFH3UG0yOCEEtV5CpC=> NFHTbZE1QCCq M2TlT4lv\HWlZYOgZZBweHSxc3nzxsA> M{XxNVI{Ozh{OUG0
HONE1 MVHBdI9xfG:|aYOgRZN{[Xl? NHXm[Y0yOCEEtV5CpC=> M3ryeFQ5KGh? NVPLUJV7cW6mdXPld{BieG:ydH;zbZPDqA>? NXTWdXFnOjN|OEK5NVQ>
CNE2 NF2wXodE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M{fsUFEwOiEQvF2= NXrHRY5jPDhiaB?= MX7z[Y5{cXSrenWgZ4VtdHNidH:gdoFlcW:2aHXyZZB6 NEnE[nEzOzN6MkmxOC=>
HONE1 NGnUelhE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NET2W2cyNzJizszN MWe0PEBp M1rSdpNmdnOrdHn6[UBk\WyuczD0c{Bz[WSrb4To[ZJieHl? NF3x[JMzOzN6MkmxOC=>
C666-1  MYHD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MVWxM|Ih|ryP MoDsOFghcA>? MYPz[Y5{cXSrenWgZ4VtdHNidH:gdoFlcW:2aHXyZZB6 NFzTXmszOzN6MkmxOC=>
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RL95-2 NHPmfWZHfW6ldHnvckBCe3OjeR?= MnXaNeKh|ryPwrC= MUKyOEBp MnXRSG1UVw>? M4f0U4Jtd2OtczD0bIUhVVWFMkCt[Y5p[W6lZXSgbY53[XOrb36geJJq\2encnXkJIJ6KDFyJTDGRnM> MlHKNlMzPjJ{MEi=
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CT26 M1TjWWZ2dmO2aX;uJGF{e2G7 NWHad5VFOjBibV2= Mnq0NUBp NVnoTo9ze3WycILld5NmeyCKR1[tbY5lfWOnZDDWSWdHKGW6cILld5Nqd28EoB?= M4j4PFI{OjN|MU[z
UM-SCC-17B M13VWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1nFV2lEPTB;Mj61OlIhyrFiMD60NFkh|ryPLDDHTVUxRTFwMke5JOKyKDBwMUm0JO69VQ>? M37mZlIzPzdyOEm5
OSC-19 MkTDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mo\1TWM2OD1|LkS4NUDDuSByLkm1N{DPxE1uIFfJOVA:OS5|Nk[gxtEhOC55N{Cg{txO NFPWUJUzOjd5MEi5PS=>
Cal33 MnX2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUPwbmhNUUN3ME2yMlI5OiEEsTCwMlQzOyEQvF2sJGdKPTB;MT6zOFkhyrFiMD6zOlMh|ryP Ml;RNlI4PzB6OUm=
UM-SCC-22B NHzUeoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3n5[mlEPTB;Mj62OFghyrFiMD61OFIh|ryPLDDHTVUxRTFwM{KwJOKyKDBwMkC0JO69VQ>? MYSyNlc4ODh7OR?=
UM-SCC-17B M2nOR2Z2dmO2aX;uJGF{e2G7 MWewMVMxKM7:TR?= MoHFNE0zPCCq NH\vSnFqdmirYnn0d{BUXEGWMzDhZ5RqfmG2aX;uJIRwe2ViYX7kJJRqdWViZHXw[Y5l\W62bIm= Mm\iNlI4PzB6OUm=
OSC-19 MW\GeY5kfGmxbjDBd5NigQ>? M2PJW|AuOzBizszN NXnLXIZxOC1{NDDo M2TDR4lvcGmkaYTzJHNVSVR|IHHjeIl3[XSrb36g[I9{\SCjbnSgeIlu\SCmZYDlcoRmdnSueR?= NVGwNnEyOjJ5N{C4PVk>
Cal33 NWnnRpNETnWwY4Tpc44hSXO|YYm= NVXnN4I{OC1|MDFOwG0> MUCwMVI1KGh? NHuxUnNqdmirYnn0d{BUXEGWMzDhZ5RqfmG2aX;uJIRwe2ViYX7kJJRqdWViZHXw[Y5l\W62bIm= MWiyNlc4ODh7OR?=
UM-SCC-22B MkjSSpVv[3Srb36gRZN{[Xl? NVTVWGp1OC1|MDFOwG0> NHrm[W8xNTJ2IHi= MXvpcohq[mm2czDTWGFVOyCjY4TpeoF1cW:wIHTvd4Uh[W6mIITpcYUh\GWyZX7k[Y51dHl? NUPsUlRbOjJ5N{C4PVk>
U-87MG NGHD[5ZE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NYexRYJ6OC1zMDFOwG0> M4LzTVczKGh? NH7VXVVFVVOR NXrQdVhpcW6qaXLpeJMh[2WubDD2bYFjcWyrdImg[I9{\SCmZYDlcoRmdnSueR?= NHXDR2wzPTR|Nk[4Ni=>
U-373MG NGn6ZYRE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NYjwOlRYOC1zMDFOwG0> M2PhRlczKGh? NELMd4pFVVOR MoHjbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? NHvRXnIzPTR|Nk[4Ni=>
SH-SY5Y NV[2UZlXS2WubDDWbYFjcWyrdImgRZN{[Xl? NUjFOm1SOC1zMDFOwG0> MVO3NkBp MVXEUXNQ MYTpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 Mli5NlU1OzZ4OEK=
Tu-9648 M3TqO2NmdGxiVnnhZoltcXS7IFHzd4F6 NX6z[Hc1OC1zMDFOwG0> M3GzSlczKGh? MnjmSG1UVw>? M{fyOolvcGmkaYTzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? M2fjSFI2PDN4Nkiy
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PCNs MVzD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MmnSNE0yOCEQvF2= M1;oNlczKGh? M3fTSGROW09? MXXpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 M4roTVI2PDN4Nkiy
PGCs M1;wS2NmdGxiVnnhZoltcXS7IFHzd4F6 NWPYfYg1OC1zMDFOwG0> MnrVO|IhcA>? NU\TUG06TE2VTx?= MYPpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 MUKyOVQ{PjZ6Mh?=
RAW264.7 M2rnemZ2dmO2aX;uJGF{e2G7 NHO5bHEyOCEQvF2= NVK1cYRmOTJiaB?= NFzjOFli[nKxZ3H0[ZMhfGinIH3SUmEh\XiycnXzd4lwdnNib3[gTmFMOixiU2TBWFEtKFOWQWSyMEBidmRiU2TBWFMhcW6mdXPl[EBjgSCGT16gZY5lKFRvMjD0c5hqdg>? M4jRXVIzPDV2NEOx
RAW264.7 NUD4SId1SXCxcITvd4l{KEG|c3H5 MXu1M|ExKM7:TR?= M3PRRlQ2KG2rbh?= Mm\C[Y5p[W6lZYOgeI95cW6|IHnu[JVk\WRiYYDvdJRwe2m|IHHu[EBOVVBibH;zdy=> M{\SPFIzPDV2NEOx
SW480 NUCzVYIzS2WubDDWbYFjcWyrdImgRZN{[Xl? NIW1enE2NzFyL{KwJO69VQ>? M1L1O|czKGh? NFKx[GNqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDv[kB1cGViQVzETEswS0RzM{OrxsBk\Wyucx?= NWPmXHE1OjF7MECzPVc>
HCT116 MmTzR4VtdCCYaXHibYxqfHliQYPzZZk> NUDsW|Y5PS9zMD:yNEDPxE1? NEP3ZYw4OiCq NGf6TXhqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDv[kB1cGViQVzETEswS0RzM{OrxsBk\Wyucx?= NGX1ZmkzOTlyMEO5Oy=>
DLD-1  NUDsRnpxS2WubDDWbYFjcWyrdImgRZN{[Xl? MVS1M|ExNzJyIN88US=> NYH6TlJZPzJiaB?= MUjpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCxZjD0bIUhSUyGSDuvR2QyOzNtwrDj[Yxtew>? Ml;tNlE6ODB|OUe=
SNU387  M{T4cmNmdGxiVnnhZoltcXS7IFHzd4F6 NYj4dGZpOjEEoN88UeKh NGHBfXQzPCCq NUnITG1temWmdXPld{Bk\WyuII\pZYJqdGm2eR?= NWPxe5pCOjF|MUG5O|U>
SNU398 NFPXbplE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NVLNZ5dCOjEEoN88UeKh M2C2NFI1KGh? MWHy[YR2[2W|IHPlcIwhfmmjYnnsbZR6 MXqyNVMyOTl5NR?=
HepG2 MWnD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NFPrN5QzOMLizszNxsA> NV3DNYN7OjRiaB?= M3XWbZJm\HWlZYOgZ4VtdCC4aXHibYxqfHl? NGDpSWszOTNzMUm3OS=>
Huh-7 M{HScWNmdGxiVnnhZoltcXS7IFHzd4F6 MWKyNOKh|ryPwrC= M{WwVVI1KGh? NGLnd5Bz\WS3Y3XzJINmdGxidnnhZoltcXS7 M2ex[VIyOzFzOUe1
VSMC MonYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVLLT5p{Oy93L{GwJO69VQ>? NXHD[281OzBibXnu NXvKNnhwTE2VTx?= M{DQNZBz\X[nboTzJHBFT0ZvIHHu[EB1cHKxbXLpck1u\WSrYYTl[EBXW02FIIDyc4xq\mW{YYTpc44hcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> M1rUcVIxQDR5M{C2
MDA-MB-231 NH7FUJJCeG:ydH;zbZMhSXO|YYm= NV\WWWVYOTBizszN MYiyOEBp MlfJSG1UV8Li NHfsfmFqdmS3Y3XzJIFxd3C2b4Ppdy=> MmHiNVcyOTRyMEW=
MDA-MB-435S MljDRZBweHSxc3nzJGF{e2G7 MkPaNVAh|ryP MkCxNlQhcA>? M{fYN2ROW00EoB?= MkfJbY5lfWOnczDhdI9xfG:|aYO= MnTVNVcyOTRyMEW=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-STAT3 / STAT3 ; 

PubMed: 25261365     


(A) PC3M-1E8 cells were treated with different doses of Stattic for 4 h. (B) PC3M-1E8 cells were treated with 10 μM Stattic for different time points. 

Survivin / c-Myc / Bcl-xl ; 

PubMed: 25261365     


Western blot analysis of STAT3 downstream targets in PC3M-1E8 cells treated with 10 μM Stattic for 24 h. 

PARP / C-PARP / Caspase-3 / C-Caspse-3 ; 

PubMed: 23382914     


NPC cells were exposed to the indicated concentrations of Stattic for 48 h; apoptotic cells were measured by western blot analysis of cleaved PARP and cleaved caspase-3. Protein levels were quantified using ImageJ software. 

25261365 23382914
Immunofluorescence
p-STAT3 / STAT3 / Survivin ; 

PubMed: 25261365     


Cellular localization of pSTAT3 and survivin (green) was observed by confocal microscopy after immunofluorescent staining of PC3M-1E8 cancer cells treated with 10 μM Stattic.

25261365
Growth inhibition assay
Cell viability; 

PubMed: 23382914     


Stattic inhibits the cell viability of NPC cells in a time-dependent manner. CNE2 cells were treated with 8 µM Stattic for the indicated times, and cell viability was measured by the MTT assay. 

23382914
ELISA
BDNF; 

PubMed: 27456333     


BDNF secretion was analyzed using ELISA in A549 and H1299 cells treated with or without Stattic. Results are shown as the mean ± S.E. n = 3; *p < 0.05, vs Control group, Student’s t-test. 

27456333

Protocol

Kinase Assay:

[1]

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High-Throughput Screening and FluorescencePolarization Assays:

Screening is performed at approximately 30 °C. The specificity ofscreening hits is validated in analogous assays for binding of thetest compounds to the SH2 domains of STAT1, STAT5, and Lck. The final concentration of buffer components used for all FP assays is 10 mM HEPES (pH 7.5), 1 mM EDTA, 0.1% Nonidet P-40, 50 mM NaCl, and 10% DMSO. The absence of dithiothreitol is essential for inhibitory activity. The sequences of the peptides are: STAT3, 5-carboxyfluorescein-GY(PO3H2)LPQTV-NH2; STAT1, 5-carboxyfluorescein-GY(PO3H2)DKPHVL; STAT5, 5-carboxyfluorescein-GY(PO3H2)LVLDKW; and Lck, 5-carboxyfluorescein-GY(PO3H2)EEIP. For specificity analysis at 30 °C, proteins are used at 150 nM (STAT1, STAT3, and STAT5). For specificity analysis at 37 °C, proteins are used at 370 nM (STAT3) or 100 nM (Lck). Proteins are incubated with test compounds in Eppendorf tubes at the indicated temperatures for 60 min prior addition of therespective 5-carboxyfluorescein labeled peptides (final concentration: 10 nM).Before measurement at room temperature, the mixtures are allowed to equilibrate for at least 30 min. Test compounds are used at the indicated concentrations diluted from a 20× stock in DMSO. Binding curves and inhibition curves are fitted with SigmaPlot. All competition curves are repeated three times in independent experiment
Cell Research:

[2]

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  • Cell lines: Ly3 cells
  • Concentrations: ~2.5 μM
  • Incubation Time: 48 h
  • Method:

    MTS


    (Only for Reference)
Animal Research:

[2]

- Collapse
  • Animal Models: Ly3 parental NOD/SCID IL2R null xenograft
  • Dosages: 3.75 mg/kg every day
  • Administration: intra tumor
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 42 mg/mL (198.87 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O
For best results, use promptly after mixing.
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 211.19
Formula

C8H5NO4S

CAS No. 19983-44-9
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC(=CC2=C1C=CS2(=O)=O)[N+](=O)[O-]

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    We need to dissolve the compound for i.p. injection, could you give me some suggestions?

  • Answer:

    S7024 Stattic can be dissolved in 2% DMSO/PEG 300/dd H2O at 8 mg/mL as a clear solution.

STAT Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID