Stattic

For research use only. Not for use in humans.

Catalog No.S7024

68 publications

Stattic Chemical Structure

Molecular Weight(MW): 211.19

Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1.

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Selleck's Stattic has been cited by 68 publications

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Choose Selective STAT Inhibitors

Biological Activity

Description Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1.
Features Stattic is the first non-peptide small molecule with inhibitory activity against STAT3 SH2 domain regardless of the STAT3 phosphorylation state in vitro.
Targets
STAT3 [1]
(Cell-free assay)
5.1 μM
In vitro

Stattic inhibits binding of a phosphotyrosine-containing peptide derived from the gp130 receptor to the STAT3 SH2 domain in a strongly temperature-dependent manner. Stattic only has a very weak effect on binding of a tyrosinephosphorylated peptide to the SH2-domain of the tyrosine kinase Lck. And it doesn’t inhibit dimerization of two other dimeric transcription factors (c-Myc/Max and Jun/Jun). It also inhibits fluorescein-labeled phosphopeptides to the SH2 domains of STAT1 and STAT5b. Stattic selectively inhibits DNA binding of STAT3 homodimers at a concentration of 10 μM. Shown to inhibit cellular phosphorylation of STAT3 at Tyr705 with little effect towards STAT1 phosphorylation at Tyr701 (in HepG2 cells) or the phosphorylation of JAK1, JAK2, and c-Src (in MDA-MB-231 and MDA-MB-235S cells). Stattic increases the apoptotic rate of STAT3-dependent breast cancer cell lines. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H9c2 MVrGeY5kfGmxbjDBd5NigQ>? Ml\TNVDjiIoQvF2= NWe2dW82POLCiXi= NHvx[I1z\X[ncoPld{B1cGViZX\m[YN1eyCxZjDJUE0zPw>? NIHiNHYzPjN|OU[zNy=>
NPC  M4nvVWZ2dmO2aX;uJGF{e2G7 NFjKeIYxNTdwNTFCuW0> MXPhZo9tcXOqZYOgSW1VNWyra3WgcY9t\WO3bHHyJIFtfGW{YYTpc45{NCCjbnSgZ4VtdCCvaXfyZZRqd25iYX7kJIlvfmG|aX;uJIlv\HWlZXSgZpkhWkuLUDDrco9kc2Sxd36= M{XVb|I2QTF3NEOw
HASMC MmjuSpVv[3Srb36gRZN{[Xl? MYqxMlI2NTVizszN M3iwd|IxKG2rbh?= NEPOeZZFVVORwrC= M4rIOYlvcGmkaYTzJJAuMFlrLWPURXQuOSx|LEWgd4lodmGuc9Mg MlX2NlU5PDl4MkK=
H9c2  NUP1[3R{TnWwY4Tpc44hSXO|YYm= NXzsWm1EOi9zMDFOwG0> NFLYWI8zKGh? MlXNSG1UV8Li NY\zb4o4[WK{b3fheIV{yqC2aHWgZ5l1d3C{b4TlZ5RqfmViZX\m[YN1eyCxZjDJUE0zPyCjZ3HpcpN1KFOK Mnm4NlU5OjB7MEe=
A431 MmC2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGfZXFAzyqEQvF2= M2rhSlLDqGh? NILtV3hjdG:la4OgSWdHNXKndnXyd4VlKGSnY4LlZZNmeyCrbjDj[YxtKH[rYXLpcIl1gQ>? MWSyOVczODR|NR?=
A431 M2f2cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWiyxsDPxE1? NGjVZ4UzyqCq M1rxXIlv[3KnYYPld{BqdiCjcH;weI9{cXNiaX7keYNm\CCkeTDzbIlsd26rbh?= NYP0XGtEOjV5MkC0N|U>
SiHa Mom3R4VtdCCYaXHibYxqfHliQYPzZZk> M2eyR|UuPzVibl2= M1HHNlI1yqCqwrC= NV33doh2e2ixd4OgcY9zeGixbH;nfUBw\iCjIIT5dIlk[WxiYYDvdJRwfGmlIHPlcIwh[W6mIHTvd4Uu\GWyZX7k[Y51KGyxc4Ogc4Yh[2WubDD2bYFjcWyrdIpCpC=> NYDXUIJuOjV3M{m2OFQ>
SiHa M2\yZ2Z2dmO2aX;uJGF{e2G7 MV[1MVc2KG6P MlLqNlTDqGkEoB?= NX;LWFFpemWmdXPld{B1cGYEoIDoc5NxcG:{eXzheIlwdiCjdDD0bIUhfHm{b4PpcoUhemW|aXT1[UA4ODYEoB?= MWiyOVU{QTZ2NB?=
ECA109 MnXHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2[5dlAuOjBizszN MYmyOOKhcMLi Ml;LTWM2OD13LkWwJO69VQ>? MmnVNlU1QTJ2OEC=
TE13 NUjKblBVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFm5XWoxNTJyIN88US=> MUWyOOKhcMLi MmTCTWM2OD14LkG1xsDPxE1? M4OxUlI2PDl{NEiw
KYSE150  NEnzVGpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYTrVHpVOC1{MDFOwG0> NXvCeHZtOjUEoHlCpC=> NVPlZnVMUUN3ME2xNk43PMLizszN NGnNUWMzPTR7MkS4NC=>
ECA109 M3m2NmNtd26xZ3XubYMhW3W{dnn2ZYwhSXO|YYm= M3\KVlAvPcLizszN NXS2VZhpOjUEoHlCpC=> NXzZRVBse3WycILld5NmeyC2aHWgZ4xwdm:pZX7pZ{Bnd3KvYYTpc44> NIjmT4ozPTR7MkS4NC=>
TE13 MXPDcI9vd2enbnnjJHN2en[rdnHsJGF{e2G7 MYWwMlXDqM7:TR?= M1vCT|I1yqCqwrC= MVTzeZBxemW|c3XzJJRp\SClbH;uc4dmdmmlIH\vdo1ifGmxbh?= NF\HNJkzPTR7MkS4NC=>
KYSE150  M{fLOmNtd26xZ3XubYMhW3W{dnn2ZYwhSXO|YYm= M2TNSlAvPcLizszN MmnxNlTDqGkEoB?= NH7MVnF{fXCycnXzd4V{KHSqZTDjcI9vd2enbnnjJIZwem2jdHnvci=> M1jkclI2PDl{NEiw
ECA109 NV\leY9tTnWwY4Tpc44hSXO|YYm= NX;JfYJROC53wrFOwG0> NXTJRWprOjUEoHlCpC=> M4i4VIVvcGGwY3XzJGlTNWmwZIXj[YQh\2WwZYLheIlwdiCxZjDEV2J{ NFjuSZgzPTR7MkS4NC=>
PC3M-1E8 MV3GeY5kfGmxbjDBd5NigQ>? M1v2S|IvPS93L{GwJO69VQ>? M2\3W|AuPCCq NIj4cZBqdmirYnn0d{B1cGViU2TBWFMh[WO2aY\heIlwdiCrbjDhJIRwe2VvIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XMEoB?= NELrOJQzPTJ4MUO2OS=>
PC3M-1E8 MWnGeY5kfGmxbjDBd5NigQ>? NUGxVo5uOTBizszN NXPIOZRMOjRiaB?= NF:0OVBld3ewcnXneYxifGW|IFLjcE15VCxic4Xyeol3cW5iYX7kJIMuVXml MnLTNlUzPjF|NkW=
PC3M-1E8 MYTGeY5kfGmxbjDBd5NigQ>? Mo\hNVAh|ryP NUDFOGhSOjRiaB?= M1Kw[IlvcGmkaYTzJGlNNTZiaX7keYNm\CCVVFHUN{Bi[3SrdnH0bY9vKGGwZDD0bIUhUUxvNj3pcoR2[2WmIGPURXQ{KGGldHn2ZZRqd25? MlW3NlUzPjF|NkW=
PC3M-1E8 MY\DcI9vd2enbnnjJHN2en[rdnHsJGF{e2G7 NVLLXWVyOi53L{WvNVAh|ryP MnHYbY5pcWKrdIRCpJRp\SClb3zvcpkh\m:{bXH0bY9vKHOrZ37p[olk[W62bIm= NYjlTI02OjV{NkGzOlU>
MDA-MB-231 NWi1OIVRTnWwY4Tpc44hSXO|YYm= MkH6NlAh|ryP MnXmNkBp NFjJXWxmgGirYnn0d{BUdmGrbDDhcoQhTS2lYXTo[ZJqdiCneIDy[ZN{cW:w M2XSdVI2OTV|M{S5
H9c2 NYDnUWUxTnWwY4Tpc44hSXO|YYm= MkPDNlAhyrWP MlfqN|AhdWmw NUHjcYxCTE2VT9Mg M2myU4Fjd2yrc3jld{Bxem:yb3\vcE1qdmS3Y3XkJGFMXCCyaH;zdIhwenmuYYTpc44h[XRiYn;0bEB{\XJ2N{OgZY5lKHSqckOwPC=> MYOyOVExPTB4Nx?=
HaCaT  NEnq[lFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH3nSokyOCEEtV2= NVPrPGhzOjBibXnu MYTEUXNQyqB? MV3lcohidmOnczDzc5Ji\mWwaXKtJIFv\CC|dX7peIlvcWJvaX7keYNm\CCpcn;3eIghcW6qaXLpeIlwdg>? NInWTW8zPTBzM{mwOy=>
Caki-1 M2PifWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3zY[lExKML3TR?= MoHNNlAhdWmw NIrPdG9FVVORwrC= NWPoT3hF\W6qYX7j[ZMhe2:{YX\lcoljNSCjbnSgd5VvcXSrbnniMYlv\HWlZXSg[5Jwf3SqIHnubIljcXSrb36= M13l[FI2ODF|OUC3
HaCaT  NFfsXJZCeG:ydH;zbZMhSXO|YYm= Ml3YNVAhyrWP NGXqNZQzOCCvaX6= NXH4WZZPTE2VT9Mg M4\0[olv[3KnYYPld{Bxem:yb4L0bY9veyCxZjDhdI9xfG:2aXOgZ4VtdHNiZIXlJJRwKHS{ZXH0cYVvfCC5aYToJJNwemGoZX7pZkBweiC|dX7peIlvcWJ? MUWyOVAyOzlyNx?=
FHL-primed hNSCs NGnaU2RE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NHXNPYcxNjB{LUWg{txO NFOzNmg4OiCq NVHKSGVKdGWjZIOgeI8hfGinIHzvd5Mhd2ZiY3XscEB3cWGkaXzpeJkh[XRiaHnnbEBkd26lZX70doF1cW:w MojuNlQ6PDV2M{S=
ELL-primed hNSCs MlLpR4VtdCCYaXHibYxqfHliQYPzZZk> NFfTfGQxNjB{LUWg{txO MUG3NkBp NVGzd3NLdGWjZIOgeI8hfGinIHzvd5Mhd2ZiY3XscEB3cWGkaXzpeJkh[XRiaHnnbEBkd26lZX70doF1cW:w MljxNlQ6PDV2M{S=
SS NVPDXGJTS2WubDDWbYFjcWyrdImgRZN{[Xl? NIjGOoQyNTFyIN88US=> M{LPWVczKGh? NFTY[GNFVVOR NULufWtM[2G3c3XzJIEh\G:|ZT3k[ZBmdmSnboSgbY5pcWKrdHnvckBw\iC2aHWgeoli[mmuaYT5xsA> MVeyOFc2PjFzMR?=
SeAx  MlruR4VtdCCYaXHibYxqfHliQYPzZZk> MWOxMVExKM7:TR?= NHHqWIM4OiCq NGniNWlFVVOR NGrKfYJk[XW|ZYOgZUBld3OnLXTldIVv\GWwdDDpcohq[mm2aX;uJI9nKHSqZTD2bYFjcWyrdIpCpC=> NXy1N2E3OjR5NU[xNVE>
HuT-78 MknoR4VtdCCYaXHibYxqfHliQYPzZZk> M13qcFEuOTBizszN NGjE[pU4OiCq MUXEUXNQ M2n2fYNifXOnczDhJIRwe2VvZHXw[Y5l\W62IHnubIljcXSrb36gc4YhfGinII\pZYJqdGm2edMg Mnv3NlQ4PTZzMUG=
CD4+ NIPJe|ZCeG:ydH;zbZMhSXO|YYm= NX3ETVVxOTEkgJpOwI0> MkXzNlQhcA>? NVTjVVl2TE2VTx?= MXfpcoR2[2W|IHHwc5B1d3OrczDzeJJwdmeueR?= NEPGRoczPDd3NkGxNS=>
MCF-7 M33VWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGPzOI8xNjR4OT2zMlc2KM7:TR?= Mo\0OUBl M3rqdpJm\HWlZYOgZ4VtdCCwdX3i[ZIhe2mpbnnmbYNidnSueR?= NWHIbGRnOjR5MkiwO|g>
MCF-7/LCC1 M3nDTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUTtUmYxOC52NkmtN{44PSEQvF2= MnTTOUBl NIrEOFJz\WS3Y3XzJINmdGxiboXtZoVzKHOrZ37p[olk[W62bIm= MXqyOFczQDB5OB?=
MCF-7/LCC9 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUGwMlQ3QS1|Lke1JO69VQ>? MVu1JIQ> NYfNXmVRemWmdXPld{Bk\WyuIH71cYJmeiC|aXfubYZq[2GwdHz5 MV2yOFczQDB5OB?=
HaCaT MljRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mny0NVAhyrWP Mn[yNlAhdWmw NEC0dm5FVVORwrC= NV\uNI1Q\W6qYX7j[ZMh\X[ncn;sbY12ey2rbnT1Z4VlKGOnbHyg[5Jwf3SqIHnubIljcXSrb36= M2fJVVI1PDJ|MUOx
HaCaT NEDWfGNCeG:ydH;zbZMhSXO|YYm= NEDlbWcyOCEEtV2= NW\udYJWOjBibXnu M3S4UmROW00EoB?= MoLn[Y5p[W6lZYOgeIhmKGGyb4D0c5Rq[yCnZn\lZ5R{KG:oIHX2[ZJwdGmvdYO= NH7LO3czPDR{M{GzNS=>
MDA-MB-231 NIPsOXdHfW6ldHnvckBCe3OjeR?= M3HxXlExKML3TR?= MY[yOEBp NIjhN5dFVVORwrC= M4TySZJm\HWlZYOgVE1UXEGWMzDlfJBz\XO|aX;u M4DB[VI1Ozd4NUi2
SUM-159 M2\ySWZ2dmO2aX;uJGF{e2G7 MVOxNEDDvU1? NWW1SVNkOjRiaB?= NF\UdG5FVVORwrC= MkPzdoVlfWOnczDQMXNVSVR|IHX4dJJme3Orb36= MmThNlQ{PzZ3OE[=
SK-BR-3 M4LiN2Z2dmO2aX;uJGF{e2G7 MYexNEDDvU1? MUiyOEBp NHvu[|NFVVORwrC= MX7y[YR2[2W|IGCtV3RCXDNiZYjwdoV{e2mxbh?= Ml3nNlQ{PzZ3OE[=
MCF7-HER2 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIHMXJMxNTFyIN88US=> NHf1OJA1QCCq NFnXVIRFVVOR NXjvVWVwcW6mdXPld{Bk\WyuIHTlZZRpKGSxc3Wg[IVx\W6mZX70cJk> MnzPNlQzQTd3MEi=
MCF7-HER2 M4ftUmZ2dmO2aX;uJGF{e2G7 MnLmOUDPxE1? NYHxcIpjOjRiaB?= MlzHSG1UVw>? NFu0W3llcW2rbnnzbIV{KFOxeD2yMEBQ[3RvNDygZY5lKHOudXeg[ZhxemW|c3nvci=> M4HVZlI1Ojl5NUC4
MCF7-HER2 NX;qbGt7TnWwY4Tpc44hSXO|YYm= NUK4ZXlwPSEQvF2= Mm\3NlQhcA>? M1LVcGROW09? MkDI[IVkemWjc3XzJJRp\SCneIDy[ZN{cW:wIHzleoVteyCxZjDFUXQhdWG{a3Xyd{whfmmvZX70bY4h[W6mIIPseYc> NGrPZ3IzPDJ7N{WwPC=>
MCF7-HER2 MlvXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkDiOUDPxE1? MUWyOEBp NITySHhFVVOR NVLZbpFb\W6qYX7j[ZMh[2WubDDndo94fGhiaX7obYJqfGmxbjDjc41jcW6nZDD3bZRpKEincnPldJRqdg>? NHnzSGQzPDJ7N{WwPC=>
HMECs  NWP4ZWRoTnWwY4Tpc44hSXO|YYm= M{j5c|ExKM7:TR?= NXfKbW5LOiCq MX\pcohq[mm2czDJSm7PucLibXXkbYF1\WRicHjvd5Bpd3K7bHH0bY9vKG:oIGPURXQyNCCVVFHUNkBidmRiU2TBWFM> NYXSbI9qOjR{MUGzNlc>
HTR8/SVneo M2m1SmZ2dmO2aX;uJGF{e2G7 NUCwcYM2OcLizszNxsA> M1jW[|HDqGh? M13aVJN2eHC{ZYPz[YQhV1OPLXnu[JVk\WRiU2TBWFMheGixc4Doc5J6dGG2aX;u NVLQO5RDOjRyNkCyOFE>
HTR8/SVneo NI\6[GdHfW6ldHnvckBCe3OjeR?= M3:0fVAvPS9zwrFOwG3DqA>? NX3GcYVHPDhiaB?= MWLy[ZN1d3KnczD0bIUh\XiycnXzd4lwdiCxZjDFMYNi\GincnnuJJN2eHC{ZYPz[YQh[nliT2PN M4HI[lI1ODZyMkSx
HTR8/SVneo MYfGeY5kfGmxbjDBd5NigQ>? Mo\2NeKh|ryPwrC= MVG0PEBp M1\hN5Nq\26rZnnjZY51dHliaX7jdoVie2W|IH3p[5JifGmxbjDifUBQW04EoB?= NFm4NlgzPDB4MEK0NS=>
C13* MmnJRZBweHSxc3nzJGF{e2G7 MlzvNE0yOCEQvF2= NUnmcnBtOjRxNEigbC=> MlXhbY5lfWOnczDhdI9xfG:|aYOgbY4h[SCmb4PlJIFv\CC2aX3lJIRmeGWwZHXueEBu[W6wZYK= NX;QPGo5OjN7NkK1OVg>
OV2008 NVPrW|VYSXCxcITvd4l{KEG|c3H5 NYXKOJRQOC1zMDFOwG0> NYTRNo9POjRxNEigbC=> M2fZNIlv\HWlZYOgZZBweHSxc3nzJIlvKGFiZH;z[UBidmRidHnt[UBl\XCnbnTlcpQhdWGwbnXy NF\NV20zOzl4MkW1PC=>
C13* M3TuZWFxd3C2b4Ppd{BCe3OjeR?= NEXnW3MzPC92ODDo MULlcohidmOnczDjbZNxdGG2aX6tbY5lfWOnZDDhdI9xfG:|aYO= MXWyN|k3OjV3OB?=
OV2008 M{DKWmFxd3C2b4Ppd{BCe3OjeR?= NWPBfHlrOjRxNEigbC=> NWHRdIxm\W6qYX7j[ZMh[2m|cHzheIlvNWmwZIXj[YQh[XCxcITvd4l{ MmfWNlM6PjJ3NUi=
W480  MnixSpVv[3Srb36gRZN{[Xl? MkDXNk42NzFyIN88US=> MVyzNEBucW5? NXLBeZNYTE2VTx?= NUDTTIJ[e2Wwc3n0bZpmeyClZXzsd{B1dyClaHXtc5Ji\GmxdHjldoFxgSCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? NH24emQzOzl|NEm3Ni=>
SW837 MlK5SpVv[3Srb36gRZN{[Xl? Mm\mNk42NzFyIN88US=> M{D6NlMxKG2rbh?= NWfIUHZpTE2VTx?= NXzVN2RGe2Wwc3n0bZpmeyClZXzsd{B1dyClaHXtc5Ji\GmxdHjldoFxgSCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? MX2yN|k{PDl5Mh?=
T24 MlPHSpVv[3Srb36gRZN{[Xl? NXz2VYlTOi9zMD:yNEDPxE1? NUPrcWR7OjRiaB?= MljLZ4F2e2W|IHTvd4Uu\GWyZX7k[Y51KGmwaHnibZRqd25ib3[geIhmKEO[Q1yxNk1qdmS3Y3XkJIlv[3KnYYPlJI9nKGmwdnHkbY5oKGOnbHzz Ml3iNlM2OjZyN{m=
CNE1 MUHGeY5kfGmxbjDBd5NigQ>? NEHKZXozOCEEtV2= MonEOFghcA>? NIqxT45jdG:la4OgeIhmKEmOLU[gbY5kemWjc3XkJJBpd3OyaH;yfYxifGmxbjDv[kBUfGG2Mx?= NEH4WlgzOzN6MkmxOC=>
CNE2 NVz0V3I3TnWwY4Tpc44hSXO|YYm= MY[yNEDDvU1? NGX5[I01QCCq MV;icI9kc3NidHjlJGlNNTZiaX7jdoVie2WmIIDoc5NxcG:{eXzheIlwdiCxZjDTeIF1Ow>? MojjNlM{QDJ7MUS=
HONE1 NVv1[mVoTnWwY4Tpc44hSXO|YYm= MojONlAhyrWP MX20PEBp NGDCWmVjdG:la4OgeIhmKEmOLU[gbY5kemWjc3XkJJBpd3OyaH;yfYxifGmxbjDv[kBUfGG2Mx?= NIXQPVgzOzN6MkmxOC=>
CNE1 M2jTPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1fkVVQh|ryP MX3zbYdvcW[rY3HueIx6KHKnZIXj[ZMh[2WubDD2bYFjcWyrdIm= MYKyN|M5OjlzNB?=
CNE1 MYPGeY5kfGmxbjDBd5NigQ>? MmXBNE0zOCEQvF2= MkK5NE01KGh? Mme5bY5pcWKrdIOgV5RifDNiYXP0bZZifGmxbjDpckBiKGSxc3WtJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK= MYSyN|M5OjlzNB?=
CNE2 MU\GeY5kfGmxbjDBd5NigQ>? NGnU[IQxNTJyIN88US=> MmHENE01KGh? MXPpcohq[mm2czDTeIF1OyCjY4TpeoF1cW:wIHnuJIEh\G:|ZT2gZY5lKHSrbXWt[IVx\W6mZX70JI1idm6nch?= MX6yN|M5OjlzNB?=
HONE1 MoX4SpVv[3Srb36gRZN{[Xl? MXGwMVIxKM7:TR?= NF\iWIExNTRiaB?= M{DES4lvcGmkaYTzJHN1[XR|IHHjeIl3[XSrb36gbY4h[SCmb4PlMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy MoPoNlM{QDJ7MUS=
CNE1 MVXD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NVTQfIszOC53LU[0JO69VQ>? NYL2c3RXPDhiaB?= NEPR[5R{fXCycnXzd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> MV[yN|M5OjlzNB?=
CNE2 MmLsR4VtdCCYaXHibYxqfHliQYPzZZk> MlnjNE42NTZ2IN88US=> MXG0PEBp NX\1ZnhCe3WycILld5NmeyClZXzsJJZq[WKrbHn0fUBqdiCjIHTvd4UuKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> MViyN|M5OjlzNB?=
HONE1 MWHD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M2jlWVAvPS14NDFOwG0> NVTVXFF{PDhiaB?= MX7zeZBxemW|c3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT2gZY5lKHSrbXWt[IVx\W6mZX70JI1idm6nch?= M3u2XFI{Ozh{OUG0
C666-1  NH3o[JJE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NUTGRmQzOC53LU[0JO69VQ>? M1i0bFQ5KGh? NYLJb4J1e3WycILld5NmeyClZXzsJJZq[WKrbHn0fUBqdiCjIHTvd4UuKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> Mnu5NlM{QDJ7MUS=
CNE1 Ml;zRZBweHSxc3nzJGF{e2G7 NWLrV5lQOTBiwsXNxsA> NXPFPXB7PDhiaB?= NX7QcVFucW6mdXPld{BieG:ydH;zbZPDqA>? NHX2cZgzOzN6MkmxOC=>
CNE2 NUnQXZBSSXCxcITvd4l{KEG|c3H5 MmTPNVAhyrWPwrC= NV3RT4FNPDhiaB?= NIP5dZpqdmS3Y3XzJIFxd3C2b4Ppd:Kh MUWyN|M5OjlzNB?=
HONE1 MYjBdI9xfG:|aYOgRZN{[Xl? Mn;hNVAhyrWPwrC= NHvxWG81QCCq NFGyO|FqdmS3Y3XzJIFxd3C2b4Ppd:Kh MV6yN|M5OjlzNB?=
CNE2 M1PzSWNmdGxiVnnhZoltcXS7IFHzd4F6 MmDoNU8zKM7:TR?= NIW5eoI1QCCq M{[3TZNmdnOrdHn6[UBk\WyuczD0c{Bz[WSrb4To[ZJieHl? M2fRT|I{Ozh{OUG0
HONE1 MX\D[YxtKF[rYXLpcIl1gSCDc4PhfS=> M3PqR|EwOiEQvF2= NWnBdIVjPDhiaB?= NWH6RWpbe2Wwc3n0bZpmKGOnbHzzJJRwKHKjZHnveIhmemGyeR?= NILCbHYzOzN6MkmxOC=>
C666-1  M4DOSmNmdGxiVnnhZoltcXS7IFHzd4F6 NXXG[4lsOS9{IN88US=> NWfWfGI{PDhiaB?= NIDDdXN{\W6|aYTpfoUh[2WubIOgeI8hemGmaX;0bIVz[XC7 NX34eJRwOjN|OEK5NVQ>
HEC-1A NETNcWJHfW6ldHnvckBCe3OjeR?= MUCxxsDPxE4EoB?= MVyyOEBp M3[x[mROW09? NEO1XVdjdG:la4OgeIhmKE2XQ{KwMYVvcGGwY3XkJIlvfmG|aX;uJJRzcWepZYLl[EBjgSBzMDWgSmJU NHTUfpIzOzJ4MkKwPC=>
RL95-2 MVfGeY5kfGmxbjDBd5NigQ>? NVzUV3FEOcLizszNxsA> MWiyOEBp M2LY[GROW09? NHTme|VjdG:la4OgeIhmKE2XQ{KwMYVvcGGwY3XkJIlvfmG|aX;uJJRzcWepZYLl[EBjgSBzMDWgSmJU NHvmPXozOzJ4MkKwPC=>
HEC-1A M4Oyc2Z2dmO2aX;uJGF{e2G7 NUHaWphvOcLizszNxsA> MoDWNlQhcA>? NIfPd2RFVVOR NGewS5VjdG:la4OgeIhmKE2XQ{KwMYVvcGGwY3XkJIlvfmG|aX;uJJRzcWepZYLl[EBjgSCHR1dCpC=> MVyyN|I3OjJyOB?=
RL95-2 NFjQdYlHfW6ldHnvckBCe3OjeR?= MnjyNeKh|ryPwrC= MWiyOEBp MonxSG1UVw>? NFLDSGxjdG:la4OgeIhmKE2XQ{KwMYVvcGGwY3XkJIlvfmG|aX;uJJRzcWepZYLl[EBjgSCHR1dCpC=> M1T6TFI{OjZ{MkC4
CT26 NUfVZ3d7TnWwY4Tpc44hSXO|YYm= NVr5d2FmOjBibV2= MUixJIg> NUXqdVY1e3WycILld5NmeyCKR1[tbY5lfWOnZDDWSWdHKGW6cILld5Nqd28EoB?= M1\TUFI{OjN|MU[z
UM-SCC-17B NVuzc3A1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGm1enRKSzVyPUKuOVYzKMLzIECuOFA6KM7:TTygS2k2OD1zLkK3PUDDuSByLkG5OEDPxE1? M4nkXFIzPzdyOEm5
OSC-19 MnTkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\2fGhKSzVyPUOuOFgyKMLzIECuPVU{KM7:TTygS2k2OD1zLkO2OkDDuSByLke3NEDPxE1? M4f1cVIzPzdyOEm5
Cal33 MnXyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn6zTWM2OD1{LkK4NkDDuSByLkSyN{DPxE1uIFfJOVA:OS5|NEmgxtEhOC5|NkOg{txO NIP3W4wzOjd5MEi5PS=>
UM-SCC-22B MkSxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoLETWM2OD1{Lk[0PEDDuSByLkW0NkDPxE1uIFfJOVA:OS5|MkCgxtEhOC5{MESg{txO NXLQZmFMOjJ5N{C4PVk>
UM-SCC-17B NVLiXJgxTnWwY4Tpc44hSXO|YYm= MVWwMVMxKM7:TR?= M3XkOFAuOjRiaB?= NX;lflBwcW6qaXLpeJMhW1SDVEOgZYN1cX[jdHnvckBld3OnIHHu[EB1cW2nIHTldIVv\GWwdHz5 M3nBflIzPzdyOEm5
OSC-19 M3nWdGZ2dmO2aX;uJGF{e2G7 MVuwMVMxKM7:TR?= MVewMVI1KGh? NVG4W4s5cW6qaXLpeJMhW1SDVEOgZYN1cX[jdHnvckBld3OnIHHu[EB1cW2nIHTldIVv\GWwdHz5 MXyyNlc4ODh7OR?=
Cal33 MWjGeY5kfGmxbjDBd5NigQ>? NX7xNZBGOC1|MDFOwG0> NIjFSokxNTJ2IHi= NH30NYtqdmirYnn0d{BUXEGWMzDhZ5RqfmG2aX;uJIRwe2ViYX7kJJRqdWViZHXw[Y5l\W62bIm= MWGyNlc4ODh7OR?=
UM-SCC-22B NYXrN2RvTnWwY4Tpc44hSXO|YYm= NVK2fXNsOC1|MDFOwG0> MVuwMVI1KGh? MkPSbY5pcWKrdIOgV3RCXDNiYXP0bZZifGmxbjDkc5NmKGGwZDD0bY1mKGSncHXu[IVvfGy7 MnrGNlI4PzB6OUm=
U-87MG MUHD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NUT5fHlOOC1zMDFOwG0> MljBO|IhcA>? NIn0bFdFVVOR NYTUcINTcW6qaXLpeJMh[2WubDD2bYFjcWyrdImg[I9{\SCmZYDlcoRmdnSueR?= NGf1[|IzPTR|Nk[4Ni=>
U-373MG MmKwR4VtdCCYaXHibYxqfHliQYPzZZk> MXWwMVExKM7:TR?= MmnLO|IhcA>? MXHEUXNQ M2H4cIlvcGmkaYTzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? M3LjZlI2PDN4Nkiy
SH-SY5Y M3\mT2NmdGxiVnnhZoltcXS7IFHzd4F6 MViwMVExKM7:TR?= M2O0OlczKGh? M1PhN2ROW09? M3HWUIlvcGmkaYTzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? MXeyOVQ{PjZ6Mh?=
Tu-9648 M{\rTmNmdGxiVnnhZoltcXS7IFHzd4F6 NUfre|U4OC1zMDFOwG0> MnjtO|IhcA>? NXKxbGo4TE2VTx?= MUHpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 MkLlNlU1OzZ4OEK=
Neuro-2a M1HJeWNmdGxiVnnhZoltcXS7IFHzd4F6 M1fXVVAuOTBizszN MmrKO|IhcA>? M2\YdWROW09? MnX3bY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? MmTUNlU1OzZ4OEK=
PCNs M2TnRWNmdGxiVnnhZoltcXS7IFHzd4F6 M1XhSlAuOTBizszN MnyxO|IhcA>? M{fDfmROW09? MofzbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? M3LSS|I2PDN4Nkiy
PGCs NYS1dYxqS2WubDDWbYFjcWyrdImgRZN{[Xl? NH75OlkxNTFyIN88US=> MmG2O|IhcA>? NGD3N|hFVVOR MofGbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? M{flXVI2PDN4Nkiy
RAW264.7 NWHme4ZUTnWwY4Tpc44hSXO|YYm= M3TINlExKM7:TR?= NVnDVox1OTJiaB?= NGfUbGVi[nKxZ3H0[ZMhfGinIH3SUmEh\XiycnXzd4lwdnNib3[gTmFMOixiU2TBWFEtKFOWQWSyMEBidmRiU2TBWFMhcW6mdXPl[EBjgSCGT16gZY5lKFRvMjD0c5hqdg>? NVPK[XhoOjJ2NUS0N|E>
RAW264.7 MVzBdI9xfG:|aYOgRZN{[Xl? MnjQOU8yOCEQvF2= M2\C[|Q2KG2rbh?= NIHIbFdmdmijbnPld{B1d3irboOgbY5lfWOnZDDhdI9xfG:|aYOgZY5lKE2PUDDsc5N{ MYqyNlQ2PDR|MR?=
SW480 MkfRR4VtdCCYaXHibYxqfHliQYPzZZk> MlTrOU8yOC9{MDFOwG0> NXL6TpY3PzJiaB?= Mny2bY5pcWKrdIOgZ4VtdCC4aXHibYxqfHlib3[geIhmKEGORFirM2NFOTN|K9MgZ4VtdHN? MnPjNlE6ODB|OUe=
HCT116 NV\GV2c6S2WubDDWbYFjcWyrdImgRZN{[Xl? MonYOU8yOC9{MDFOwG0> M1qxfVczKGh? NVvQR5AxcW6qaXLpeJMh[2WubDD2bYFjcWyrdImgc4YhfGinIFHMSGgsN0OGMUOzL:Kh[2WubIO= MkSyNlE6ODB|OUe=
DLD-1  Mk\UR4VtdCCYaXHibYxqfHliQYPzZZk> MljXOU8yOC9{MDFOwG0> M1v3fFczKGh? MX;pcohq[mm2czDj[YxtKH[rYXLpcIl1gSCxZjD0bIUhSUyGSDuvR2QyOzNtwrDj[Yxtew>? MYeyNVkxODN7Nx?=
SNU387  MonZR4VtdCCYaXHibYxqfHliQYPzZZk> MWOyNOKh|ryPwrC= MnSyNlQhcA>? MkXndoVlfWOnczDj[YxtKH[rYXLpcIl1gQ>? M{OwVFIyOzFzOUe1
SNU398 NVrVO3I1S2WubDDWbYFjcWyrdImgRZN{[Xl? M4PnO|IxyqEQvF5CpC=> NHW0U2czPCCq NXLzVmFMemWmdXPld{Bk\WyuII\pZYJqdGm2eR?= NFL1WmUzOTNzMUm3OS=>
HepG2 NVTzWHdyS2WubDDWbYFjcWyrdImgRZN{[Xl? NH34XlEzOMLizszNxsA> NHLLOXEzPCCq MUXy[YR2[2W|IHPlcIwhfmmjYnnsbZR6 NWWybWlEOjF|MUG5O|U>
Huh-7 NInzeFlE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M2LsflIxyqEQvF5CpC=> MnyyNlQhcA>? NXf4XVV6emWmdXPld{Bk\WyuII\pZYJqdGm2eR?= M1;DZVIyOzFzOUe1
VSMC M{LTemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHrjZng{NzVxMUCg{txO NG[2c48{OCCvaX6= MoLQSG1UVw>? NYDMRpN5eHKndnXueJMhWESJRj2gZY5lKHSqcn;tZolvNW2nZHnheIVlKF[VTVOgdJJwdGmoZYLheIlwdiCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? Moe0NlA5PDd|ME[=
MDA-MB-231 M2fFTGFxd3C2b4Ppd{BCe3OjeR?= M1zuZ|ExKM7:TR?= Mn\uNlQhcA>? NHnsNGtFVVORwrC= M{\4Xolv\HWlZYOgZZBweHSxc3nz NX7IdVFGOTdzMUSwNFU>
MDA-MB-435S NEHrc3VCeG:ydH;zbZMhSXO|YYm= M{XpNlExKM7:TR?= NHqxTmUzPCCq NXHycYhRTE2VT9Mg NIXXbXNqdmS3Y3XzJIFxd3C2b4Ppdy=> M3\HVVE4OTF2MEC1

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-STAT3 / STAT3 ; 

PubMed: 25261365     


(A) PC3M-1E8 cells were treated with different doses of Stattic for 4 h. (B) PC3M-1E8 cells were treated with 10 μM Stattic for different time points. 

Survivin / c-Myc / Bcl-xl ; 

PubMed: 25261365     


Western blot analysis of STAT3 downstream targets in PC3M-1E8 cells treated with 10 μM Stattic for 24 h. 

PARP / C-PARP / Caspase-3 / C-Caspse-3 ; 

PubMed: 23382914     


NPC cells were exposed to the indicated concentrations of Stattic for 48 h; apoptotic cells were measured by western blot analysis of cleaved PARP and cleaved caspase-3. Protein levels were quantified using ImageJ software. 

25261365 23382914
Immunofluorescence
p-STAT3 / STAT3 / Survivin ; 

PubMed: 25261365     


Cellular localization of pSTAT3 and survivin (green) was observed by confocal microscopy after immunofluorescent staining of PC3M-1E8 cancer cells treated with 10 μM Stattic.

25261365
Growth inhibition assay
Cell viability; 

PubMed: 23382914     


Stattic inhibits the cell viability of NPC cells in a time-dependent manner. CNE2 cells were treated with 8 µM Stattic for the indicated times, and cell viability was measured by the MTT assay. 

23382914
ELISA
BDNF; 

PubMed: 27456333     


BDNF secretion was analyzed using ELISA in A549 and H1299 cells treated with or without Stattic. Results are shown as the mean ± S.E. n = 3; *p < 0.05, vs Control group, Student’s t-test. 

27456333

Protocol

Kinase Assay:

[1]

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High-Throughput Screening and FluorescencePolarization Assays:

Screening is performed at approximately 30 °C. The specificity ofscreening hits is validated in analogous assays for binding of thetest compounds to the SH2 domains of STAT1, STAT5, and Lck. The final concentration of buffer components used for all FP assays is 10 mM HEPES (pH 7.5), 1 mM EDTA, 0.1% Nonidet P-40, 50 mM NaCl, and 10% DMSO. The absence of dithiothreitol is essential for inhibitory activity. The sequences of the peptides are: STAT3, 5-carboxyfluorescein-GY(PO3H2)LPQTV-NH2; STAT1, 5-carboxyfluorescein-GY(PO3H2)DKPHVL; STAT5, 5-carboxyfluorescein-GY(PO3H2)LVLDKW; and Lck, 5-carboxyfluorescein-GY(PO3H2)EEIP. For specificity analysis at 30 °C, proteins are used at 150 nM (STAT1, STAT3, and STAT5). For specificity analysis at 37 °C, proteins are used at 370 nM (STAT3) or 100 nM (Lck). Proteins are incubated with test compounds in Eppendorf tubes at the indicated temperatures for 60 min prior addition of therespective 5-carboxyfluorescein labeled peptides (final concentration: 10 nM).Before measurement at room temperature, the mixtures are allowed to equilibrate for at least 30 min. Test compounds are used at the indicated concentrations diluted from a 20× stock in DMSO. Binding curves and inhibition curves are fitted with SigmaPlot. All competition curves are repeated three times in independent experiment
Cell Research:

[2]

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  • Cell lines: Ly3 cells
  • Concentrations: ~2.5 μM
  • Incubation Time: 48 h
  • Method:

    MTS


    (Only for Reference)
Animal Research:

[2]

- Collapse
  • Animal Models: Ly3 parental NOD/SCID IL2R null xenograft
  • Formulation: --
  • Dosages: 3.75 mg/kg every day
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 42 mg/mL (198.87 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+H2O
For best results, use promptly after mixing.
8mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 211.19
Formula

C8H5NO4S

CAS No. 19983-44-9
Storage powder
in solvent
Synonyms N/A
Smiles [O-][N+](=O)C1=CC=C2C=C[S](=O)(=O)C2=C1

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
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    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    We need to dissolve the compound for i.p. injection, could you give me some suggestions?

  • Answer:

    S7024 Stattic can be dissolved in 2% DMSO/PEG 300/dd H2O at 8 mg/mL as a clear solution.

STAT Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID