Stattic

For research use only.

Catalog No.S7024

125 publications

Stattic Chemical Structure

CAS No. 19983-44-9

Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1. Stattic induces apoptosis.

Size Price Stock Quantity  
10mM (1mL in DMSO) USD 90 In stock
USD 110 In stock
USD 170 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's Stattic has been cited by 125 publications

Purity & Quality Control

Choose Selective STAT Inhibitors

Biological Activity

Description Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1. Stattic induces apoptosis.
Features Stattic is the first non-peptide small molecule with inhibitory activity against STAT3 SH2 domain regardless of the STAT3 phosphorylation state in vitro.
Targets
STAT3 [1]
(Cell-free assay)
5.1 μM
In vitro

Stattic inhibits binding of a phosphotyrosine-containing peptide derived from the gp130 receptor to the STAT3 SH2 domain in a strongly temperature-dependent manner. Stattic only has a very weak effect on binding of a tyrosinephosphorylated peptide to the SH2-domain of the tyrosine kinase Lck. And it doesn’t inhibit dimerization of two other dimeric transcription factors (c-Myc/Max and Jun/Jun). It also inhibits fluorescein-labeled phosphopeptides to the SH2 domains of STAT1 and STAT5b. Stattic selectively inhibits DNA binding of STAT3 homodimers at a concentration of 10 μM. Shown to inhibit cellular phosphorylation of STAT3 at Tyr705 with little effect towards STAT1 phosphorylation at Tyr701 (in HepG2 cells) or the phosphorylation of JAK1, JAK2, and c-Src (in MDA-MB-231 and MDA-MB-235S cells). Stattic increases the apoptotic rate of STAT3-dependent breast cancer cell lines. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H9c2 M3nTOmZ2dmO2aX;uJGF{e2G7 NH3ZXlkyOOLCid88US=> NEP1VYY16oDLaB?= MV7y[ZZmenOnczD0bIUh\W[oZXP0d{Bw\iCLTD2yOy=> MnnmNlY{Ozl4M{O=
NPC  NELiWGtHfW6ldHnvckBCe3OjeR?= NUHmTol6OC15LkWgxtVO MXXhZo9tcXOqZYOgSW1VNWyra3WgcY9t\WO3bHHyJIFtfGW{YYTpc45{NCCjbnSgZ4VtdCCvaXfyZZRqd25iYX7kJIlvfmG|aX;uJIlv\HWlZXSgZpkhWkuLUDDrco9kc2Sxd36= NWi4Z5V1OjV7MUW0N|A>
HASMC NIS0fJVHfW6ldHnvckBCe3OjeR?= MUWxMlI2NTVizszN NXT4bJRFOjBibXnu NYrIWYl4TE2VT9Mg NI\PWVlqdmirYnn0d{BxNSi\KT3TWGFVNTFuMzy1JJNq\26jbIRCpC=> NF\Eb5MzPTh2OU[yNi=>
H9c2  MorPSpVv[3Srb36gRZN{[Xl? NFvnOYMzNzFyIN88US=> MWqyJIg> Mk\sSG1UV8Li MYDhZpJw\2G2ZYRCpJRp\SCleYTvdJJwfGWldHn2[UBm\m[nY4TzJI9nKEmOLUK3JIFo[Wmwc4SgV2g> MXGyOVgzODlyNx?=
A431 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{PLcFLDqM7:TR?= Ml;vNuKhcA>? MXPicI9kc3NiRVfGMZJmfmW{c3XkJIRm[3KnYYPld{BqdiClZXzsJJZq[WKrbHn0fS=> NVLISFF2OjV5MkC0N|U>
A431 MlrMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYTCdZFFOsLizszN M4HBUVLDqGh? MorObY5kemWjc3XzJIlvKGGyb4D0c5NqeyCrbnT1Z4VlKGK7IIPobYtwdmmw NH\iToYzPTd{MESzOS=>
SiHa NHLabo9E\WyuIG\pZYJqdGm2eTDBd5NigQ>? NV36O3BsPS15NTDuUS=> NXXl[JY{OjUEoHlCpC=> MYPzbI94eyCvb4LwbI9td2e7IH;mJIEhfHmyaXPhcEBieG:ydH;0bYMh[2WubDDhcoQh\G:|ZT3k[ZBmdmSnboSgcI9{eyCxZjDj[YxtKH[rYXLpcIl1gcLi Ml62NlU2Ozl4NES=
SiHa NITHcYlHfW6ldHnvckBCe3OjeR?= NXXFOFgxPS15NTDuUS=> MUCyOOKhcMLi NGrQcXVz\WS3Y3XzJJRp\cLicHjvd5Bpd3K7bHH0bY9vKGG2IITo[UB1gXKxc3nu[UBz\XOrZIXlJFcxPcLi NYXwfmd3OjV3M{m2OFQ>
ECA109 MlzQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4XufVAuOjBizszN NVrlc|A1OjUEoHlCpC=> MoKyTWM2OD13LkWwJO69VQ>? MXSyOVQ6OjR6MB?=
TE13 M4j2[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXSwMVIxKM7:TR?= NVfYXWJ[OjUEoHlCpC=> NULme3g6UUN3ME22MlE2yqEQvF2= M1HYV|I2PDl{NEiw
KYSE150  M3HlUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2PUWlAuOjBizszN NGHBTlgzPMLiaNMg MXTJR|UxRTF{Lk[0xsDPxE1? NU[5OVZjOjV2OUK0PFA>
ECA109 NYfMOGVVS2yxbn;n[Y5q[yCVdYL2bZZidCCDc4PhfS=> NVTUPXE4OC53wrFOwG0> MnfoNlTDqGkEoB?= NWnkWJJFe3WycILld5NmeyC2aHWgZ4xwdm:pZX7pZ{Bnd3KvYYTpc44> NVThfVdSOjV2OUK0PFA>
TE13 MVjDcI9vd2enbnnjJHN2en[rdnHsJGF{e2G7 NELsT4QxNjYEoN88US=> MXmyOOKhcMLi MV;zeZBxemW|c3XzJJRp\SClbH;uc4dmdmmlIH\vdo1ifGmxbh?= MXyyOVQ6OjR6MB?=
KYSE150  Ml:0R4xwdm:pZX7pZ{BUfXK4aY\hcEBCe3OjeR?= M4jaTlAvPcLizszN MVOyOOKhcMLi Ml;Kd5VxeHKnc4Pld{B1cGViY3zvco9o\W6rYzDmc5Ju[XSrb36= NELHN2kzPTR7MkS4NC=>
ECA109 NEnFN3FHfW6ldHnvckBCe3OjeR?= MYCwMlXDqM7:TR?= MYqyOOKhcMLi M{TPToVvcGGwY3XzJGlTNWmwZIXj[YQh\2WwZYLheIlwdiCxZjDEV2J{ NIPBPY0zPTR7MkS4NC=>
PC3M-1E8 MUfGeY5kfGmxbjDBd5NigQ>? MlS4Nk42NzVxMUCg{txO NUTKU3R[OC12IHi= NWfuPWFOcW6qaXLpeJMhfGinIGPURXQ{KGGldHn2ZZRqd25iaX6gZUBld3OnLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{wrC= MlvmNlUzPjF|NkW=
PC3M-1E8 Ml7PSpVv[3Srb36gRZN{[Xl? MXWxNEDPxE1? M1TnNVI1KGh? M2myVIRwf26{ZXf1cIF1\XNiQnPsMZhNNCC|dYL2bZZqdiCjbnSgZ{1OgWN? MU[yOVI3OTN4NR?=
PC3M-1E8 MoHFSpVv[3Srb36gRZN{[Xl? MonqNVAh|ryP NXHDO3JkOjRiaB?= M{OzNYlvcGmkaYTzJGlNNTZiaX7keYNm\CCVVFHUN{Bi[3SrdnH0bY9vKGGwZDD0bIUhUUxvNj3pcoR2[2WmIGPURXQ{KGGldHn2ZZRqd25? MkTnNlUzPjF|NkW=
PC3M-1E8 Mor2R4xwdm:pZX7pZ{BUfXK4aY\hcEBCe3OjeR?= MmnTNk42NzVxMUCg{txO NX3CZo9lcW6qaXLpeJPDqHSqZTDjc4xwdnliZn;ycYF1cW:wIIPp[45q\mmlYX70cJk> M3PzUlI2OjZzM{[1
MDA-MB-231 NF:yW4dHfW6ldHnvckBCe3OjeR?= MkWwNlAh|ryP NUjZbJJsOiCq M1\3cYV5cGmkaYTzJHNv[WmuIHHu[EBGNWOjZHjldolvKGW6cILld5Nqd25? NYLxZ5Z1OjVzNUOzOFk>
H9c2 MoLQSpVv[3Srb36gRZN{[Xl? Mn3YNlAhyrWP MXKzNEBucW5? M3HUWmROW00EoB?= MonvZYJwdGm|aHXzJJBzd3CxZn;sMYlv\HWlZXSgRWtVKHCqb4PwbI9zgWyjdHnvckBifCCkb4ToJJNmejR5MzDhcoQhfGi{M{C4 NXTzNm44OjVzMEWwOlc>
HaCaT  Ml3XS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mlf3NVAhyrWP MUWyNEBucW5? MljPSG1UV8Li NUPwS2N2\W6qYX7j[ZMhe2:{YX\lcoljNSCjbnSgd5VvcXSrbnniMYlv\HWlZXSg[5Jwf3SqIHnubIljcXSrb36= M1zDeVI2ODF|OUC3
Caki-1 NYnPNYV{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUKxNEDDvU1? NEnq[ZUzOCCvaX6= Mm[0SG1UV8Li M2G3TIVvcGGwY3XzJJNwemGoZX7pZk0h[W6mIIP1col1cW6rYj3pcoR2[2WmIHfyc5d1cCCrbnjpZol1cW:w MmnFNlUxOTN7MEe=
HaCaT  MlT6RZBweHSxc3nzJGF{e2G7 MmrTNVAhyrWP M2C5clIxKG2rbh?= NV;nTpJGTE2VT9Mg MYrpcoNz\WG|ZYOgdJJweG:{dHnvcpMhd2ZiYYDvdJRwfGmlIHPlcIx{KGS3ZTD0c{B1emWjdH3lcpQhf2m2aDDzc5Ji\mWwaXKgc5Ihe3WwaYTpcolj Mo\3NlUxOTN7MEe=
FHL-primed hNSCs NYfaS2VbS2WubDDWbYFjcWyrdImgRZN{[Xl? MVewMlAzNTVizszN M{nUUlczKGh? NFrXZ3dt\WGmczD0c{B1cGVibH;zd{Bw\iClZXzsJJZq[WKrbHn0fUBifCCqaXfoJINwdmOnboTyZZRqd25? MmPsNlQ6PDV2M{S=
ELL-primed hNSCs MmDrR4VtdCCYaXHibYxqfHliQYPzZZk> MkXQNE4xOi13IN88US=> NIPxe|c4OiCq NELVTHVt\WGmczD0c{B1cGVibH;zd{Bw\iClZXzsJJZq[WKrbHn0fUBifCCqaXfoJINwdmOnboTyZZRqd25? MoXmNlQ6PDV2M{S=
SS MY\D[YxtKF[rYXLpcIl1gSCDc4PhfS=> NHTLOngyNTFyIN88US=> NYPaU5BlPzJiaB?= NHvaTpVFVVOR M3vl[4NifXOnczDhJIRwe2VvZHXw[Y5l\W62IHnubIljcXSrb36gc4YhfGinII\pZYJqdGm2edMg NU\4WlBvOjR5NU[xNVE>
SeAx  MWTD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MlPLNU0yOCEQvF2= NH;0OZY4OiCq MVrEUXNQ M1[zSoNifXOnczDhJIRwe2VvZHXw[Y5l\W62IHnubIljcXSrb36gc4YhfGinII\pZYJqdGm2edMg MXeyOFc2PjFzMR?=
HuT-78 MVvD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NV32SHNKOS1zMDFOwG0> M3;1WlczKGh? M2HUWWROW09? NYeyU3hG[2G3c3XzJIEh\G:|ZT3k[ZBmdmSnboSgbY5pcWKrdHnvckBw\iC2aHWgeoli[mmuaYT5xsA> NWX4eGQ1OjR5NU[xNVE>
CD4+ NIn3N3JCeG:ydH;zbZMhSXO|YYm= MlniNVDjiIoQvH2= NEjoTI0zPCCq NVP0T5B4TE2VTx?= MUnpcoR2[2W|IHHwc5B1d3OrczDzeJJwdmeueR?= NXjsfI9OOjR5NU[xNVE>
MCF-7 M1LLTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmXENE41PjlvMz63OUDPxE1? MYK1JIQ> MUHy[YR2[2W|IHPlcIwhdnWvYnXyJJNq\26rZnnjZY51dHl? MXGyOFczQDB5OB?=
MCF-7/LCC1 NGXXNWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NELpWYcxNjR4OT2zMlc2KM7:TR?= MWi1JIQ> M3XBc5Jm\HWlZYOgZ4VtdCCwdX3i[ZIhe2mpbnnmbYNidnSueR?= M3e5eVI1PzJ6MEe4
MCF-7/LCC9 NIH4XopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUGwMlQ3QS1|Lke1JO69VQ>? NXn1cYI6PSCm NHTMXZFz\WS3Y3XzJINmdGxiboXtZoVzKHOrZ37p[olk[W62bIm= NGLOS2ozPDd{OEC3PC=>
HaCaT MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2fPO|ExKML3TR?= NYPPN484OjBibXnu M4HrWWROW00EoB?= Mlj0[Y5p[W6lZYOg[ZZmem:uaX31d{1qdmS3Y3XkJINmdGxiZ4Lve5RpKGmwaHnibZRqd25? NVX4T3FXOjR2MkOxN|E>
HaCaT MYrBdI9xfG:|aYOgRZN{[Xl? MnvaNVAhyrWP MUOyNEBucW5? MWfEUXNQyqB? NGTZTpNmdmijbnPld{B1cGViYYDvdJRwfGmlIHXm[oVkfHNib3[g[ZZmem:uaX31dy=> NHHvcYEzPDR{M{GzNS=>
MDA-MB-231 MmHmSpVv[3Srb36gRZN{[Xl? M{jOXVExKML3TR?= Ml7ZNlQhcA>? NFLOZmtFVVORwrC= M4Dsb5Jm\HWlZYOgVE1UXEGWMzDlfJBz\XO|aX;u Mm\RNlQ{PzZ3OE[=
SUM-159 MYPGeY5kfGmxbjDBd5NigQ>? MVuxNEDDvU1? NHvUcY0zPCCq M2PPPGROW00EoB?= NHTZdWhz\WS3Y3XzJHAuW1SDVEOg[ZhxemW|c3nvci=> Mn3KNlQ{PzZ3OE[=
SK-BR-3 NFK5fXZHfW6ldHnvckBCe3OjeR?= MXixNEDDvU1? NHPYWmgzPCCq NYDYXHBrTE2VT9Mg Moj3doVlfWOnczDQMXNVSVR|IHX4dJJme3Orb36= MWKyOFM4PjV6Nh?=
MCF7-HER2 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MojuNE0yOCEQvF2= M1HwelQ5KGh? MYLEUXNQ NF3oU3VqdmS3Y3XzJINmdGxiZHXheIgh\G:|ZTDk[ZBmdmSnboTsfS=> NITwSGkzPDJ7N{WwPC=>
MCF7-HER2 NGnSb|JHfW6ldHnvckBCe3OjeR?= M1jhOlUh|ryP MljsNlQhcA>? NFPEVZJFVVOR MnG4[IlucW6rc3jld{BUd3hvMjygU4N1NTRuIHHu[EB{dHWpIHX4dJJme3Orb36= MVuyOFI6PzVyOB?=
MCF7-HER2 NEXnRXpHfW6ldHnvckBCe3OjeR?= NYLxPWFbPSEQvF2= MYGyOEBp MWTEUXNQ NXPTdWZH\GWlcnXhd4V{KHSqZTDlfJBz\XO|aX;uJIxmfmWuczDv[kBGVVRibXHyb4Vzeyxidnnt[Y51cW5iYX7kJJNtfWd? M4fRNFI1Ojl5NUC4
MCF7-HER2 MmP3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIq1PXQ2KM7:TR?= MlP0NlQhcA>? MmrUSG1UVw>? NEXCVYpmdmijbnPld{Bk\WyuIHfyc5d1cCCrbnjpZol1cW:wIHPvcYJqdmWmIIfpeIghUGW{Y3XweIlv M{\kW|I1Ojl5NUC4
HMECs  NGftWpNHfW6ldHnvckBCe3OjeR?= NULabJo6OTBizszN MXOyJIg> MWHpcohq[mm2czDJSm7PucLibXXkbYF1\WRicHjvd5Bpd3K7bHH0bY9vKG:oIGPURXQyNCCVVFHUNkBidmRiU2TBWFM> Mkm5NlQzOTF|Mke=
HTR8/SVneo MXLGeY5kfGmxbjDBd5NigQ>? M2\JZlHDqM7:TdMg MWexxsBp MnLvd5VxeHKnc4Pl[EBQW01vaX7keYNm\CCVVFHUN{BxcG:|cHjvdplt[XSrb36= NWrmN5B4OjRyNkCyOFE>
HTR8/SVneo M2ToeWZ2dmO2aX;uJGF{e2G7 NVrMV5oxOC53L{JCpO69VcLi NVHCT|A5PDhiaB?= M4rpU5Jme3SxcnXzJJRp\SCneIDy[ZN{cW:wIH;mJGUu[2GmaHXybY4he3WycILld5Nm\CCkeTDPV20> NFfvfY0zPDB4MEK0NS=>
HTR8/SVneo M2DPOGZ2dmO2aX;uJGF{e2G7 Mlf0NeKh|ryPwrC= NFLzW2c1QCCq Ml71d4lodmmoaXPhcpRtgSCrbnPy[YF{\XNibXnndoF1cW:wIHL5JG9UVcLi NX3NdINKOjRyNkCyOFE>
C13* NYi1bJA4SXCxcITvd4l{KEG|c3H5 MmXiNE0yOCEQvF2= MYeyOE81QCCq M4\lUIlv\HWlZYOgZZBweHSxc3nzJIlvKGFiZH;z[UBidmRidHnt[UBl\XCnbnTlcpQhdWGwbnXy NITqbHUzOzl4MkW1PC=>
OV2008 M3XSWmFxd3C2b4Ppd{BCe3OjeR?= MlrLNE0yOCEQvF2= M2PGOVI1NzR6IHi= NHjTc4hqdmS3Y3XzJIFxd3C2b4Ppd{BqdiCjIHTvd4Uh[W6mIITpcYUh\GWyZX7k[Y51KG2jbn7ldi=> MUWyN|k3OjV3OB?=
C13* MXfBdI9xfG:|aYOgRZN{[Xl? Mm\0NlQwPDhiaB?= MXLlcohidmOnczDjbZNxdGG2aX6tbY5lfWOnZDDhdI9xfG:|aYO= NUPuNHlbOjN7NkK1OVg>
OV2008 Mnn1RZBweHSxc3nzJGF{e2G7 MX2yOE81QCCq NIDmeIRmdmijbnPld{BkcXOybHH0bY4ucW6mdXPl[EBieG:ydH;zbZM> MXGyN|k3OjV3OB?=
W480  Mlq1SpVv[3Srb36gRZN{[Xl? MVqyMlUwOTBizszN NIrDblM{OCCvaX6= NVi5XmZvTE2VTx?= M2ezZ5NmdnOrdHn6[ZMh[2WubIOgeI8h[2inbX;yZYRqd3SqZYLhdJkhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NIOwb24zOzl|NEm3Ni=>
SW837 NHq5U3JHfW6ldHnvckBCe3OjeR?= NF;ZeoIzNjVxMUCg{txO NFTFT5k{OCCvaX6= NEXCenRFVVOR NH\oVHZ{\W6|aYTpfoV{KGOnbHzzJJRwKGOqZX3vdoFlcW:2aHXyZZB6KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz MWiyN|k{PDl5Mh?=
T24 NHnje5FHfW6ldHnvckBCe3OjeR?= M1PKUlIwOTBxMkCg{txO NIG1c|UzPCCq Mn\HZ4F2e2W|IHTvd4Uu\GWyZX7k[Y51KGmwaHnibZRqd25ib3[geIhmKEO[Q1yxNk1qdmS3Y3XkJIlv[3KnYYPlJI9nKGmwdnHkbY5oKGOnbHzz M1zUdVI{PTJ4MEe5
CNE1 MX3GeY5kfGmxbjDBd5NigQ>? M3fYTFIxKML3TR?= NEnwbnU1QCCq NV;qZ2o1[myxY3vzJJRp\SCLTD22JIlv[3KnYYPl[EBxcG:|cHjvdplt[XSrb36gc4YhW3SjdEO= NIDVdIgzOzN6MkmxOC=>
CNE2 NVrSepR7TnWwY4Tpc44hSXO|YYm= M2PCOVIxKML3TR?= MVS0PEBp MX\icI9kc3NidHjlJGlNNTZiaX7jdoVie2WmIIDoc5NxcG:{eXzheIlwdiCxZjDTeIF1Ow>? NFXTdGszOzN6MkmxOC=>
HONE1 NYLvb5ByTnWwY4Tpc44hSXO|YYm= NIDucpYzOCEEtV2= NVLVPJF{PDhiaB?= NIPIcZljdG:la4OgeIhmKEmOLU[gbY5kemWjc3XkJJBpd3OyaH;yfYxifGmxbjDv[kBUfGG2Mx?= Ml7pNlM{QDJ7MUS=
CNE1 M13MNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{P4fVQh|ryP MmPvd4lodmmoaXPhcpRtgSC{ZXT1Z4V{KGOnbHygeoli[mmuaYT5 MnrSNlM{QDJ7MUS=
CNE1 MUnGeY5kfGmxbjDBd5NigQ>? NGXkU3IxNTJyIN88US=> MkizNE01KGh? NV7Mdnp{cW6qaXLpeJMhW3SjdEOgZYN1cX[jdHnvckBqdiCjIHTvd4UuKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> NILhNGwzOzN6MkmxOC=>
CNE2 MVXGeY5kfGmxbjDBd5NigQ>? MYiwMVIxKM7:TR?= M1fTcVAuPCCq MnS1bY5pcWKrdIOgV5RifDNiYXP0bZZifGmxbjDpckBiKGSxc3WtJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK= M{LJWVI{Ozh{OUG0
HONE1 MkOySpVv[3Srb36gRZN{[Xl? MlnwNE0zOCEQvF2= MUWwMVQhcA>? NECwcY5qdmirYnn0d{BUfGG2MzDhZ5RqfmG2aX;uJIlvKGFiZH;z[U0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> M2nQTlI{Ozh{OUG0
CNE1 M{P0NWNmdGxiVnnhZoltcXS7IFHzd4F6 MWiwMlUuPjRizszN MmC5OFghcA>? NVXWZlRie3WycILld5NmeyClZXzsJJZq[WKrbHn0fUBqdiCjIHTvd4UuKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> NXrweGF{OjN|OEK5NVQ>
CNE2 M{n4bGNmdGxiVnnhZoltcXS7IFHzd4F6 NHrn[IkxNjVvNkSg{txO MmfCOFghcA>? M{TC[ZN2eHC{ZYPz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy MojsNlM{QDJ7MUS=
HONE1 MV7D[YxtKF[rYXLpcIl1gSCDc4PhfS=> M1G5UlAvPS14NDFOwG0> NXTGU5VUPDhiaB?= NXmzeo1ie3WycILld5NmeyClZXzsJJZq[WKrbHn0fUBqdiCjIHTvd4UuKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> NXXwbJVQOjN|OEK5NVQ>
C666-1  MWHD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NUTsSGFxOC53LU[0JO69VQ>? M2PTZ|Q5KGh? Mm\md5VxeHKnc4Pld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3WtJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK= NHXKdYEzOzN6MkmxOC=>
CNE1 M3fFTGFxd3C2b4Ppd{BCe3OjeR?= MoDTNVAhyrWPwrC= NYL0Onh7PDhiaB?= NHLVOm5qdmS3Y3XzJIFxd3C2b4Ppd:Kh M33IUFI{Ozh{OUG0
CNE2 NHfNPXlCeG:ydH;zbZMhSXO|YYm= NXzKcmJFOTBiwsXNxsA> M2P0R|Q5KGh? M2TNVYlv\HWlZYOgZZBweHSxc3nzxsA> NIT2ZZAzOzN6MkmxOC=>
HONE1 MojmRZBweHSxc3nzJGF{e2G7 MXmxNEDDvU4EoB?= MUG0PEBp MkHGbY5lfWOnczDhdI9xfG:|aYRCpC=> MVuyN|M5OjlzNB?=
CNE2 M1\GWGNmdGxiVnnhZoltcXS7IFHzd4F6 M365NVEwOiEQvF2= NHnpSHE1QCCq NEXvcHF{\W6|aYTpfoUh[2WubIOgeI8hemGmaX;0bIVz[XC7 NXzJfWJCOjN|OEK5NVQ>
HONE1 MVnD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MmDDNU8zKM7:TR?= Mn7JOFghcA>? Ml3Nd4Vve2m2aYrlJINmdGy|IITvJJJi\GmxdHjldoFxgQ>? MYWyN|M5OjlzNB?=
C666-1  NVHaN5Y5S2WubDDWbYFjcWyrdImgRZN{[Xl? MV6xM|Ih|ryP MoC4OFghcA>? NGTBZlh{\W6|aYTpfoUh[2WubIOgeI8hemGmaX;0bIVz[XC7 NEK3[YwzOzN6MkmxOC=>
HEC-1A NXLIXJNSTnWwY4Tpc44hSXO|YYm= NUDnVYx5OcLizszNxsA> M{LPflI1KGh? MoniSG1UVw>? NHTkPJljdG:la4OgeIhmKE2XQ{KwMYVvcGGwY3XkJIlvfmG|aX;uJJRzcWepZYLl[EBjgSBzMDWgSmJU M{H2VVI{OjZ{MkC4
RL95-2 MnrwSpVv[3Srb36gRZN{[Xl? NGHqdGgyyqEQvF5CpC=> M{PjVlI1KGh? NF3qbHBFVVOR MlniZoxw[2u|IITo[UBOXUN{MD3lcohidmOnZDDpcpZie2mxbjD0dolo\2W{ZXSgZpkhOTBnIF\CVy=> MnvyNlMzPjJ{MEi=
HEC-1A NYXwN4t7TnWwY4Tpc44hSXO|YYm= NIXW[oEyyqEQvF5CpC=> M3v5VVI1KGh? MmDzSG1UVw>? MVjicI9kc3NidHjlJG1WSzJyLXXubIFv[2WmIHnueoF{cW:wIITybYdo\XKnZDDifUBGT0cEoB?= M125XlI{OjZ{MkC4
RL95-2 NWmwZZdGTnWwY4Tpc44hSXO|YYm= NUXKUW9bOcLizszNxsA> NWn1U4s2OjRiaB?= MWrEUXNQ NFvjbHVjdG:la4OgeIhmKE2XQ{KwMYVvcGGwY3XkJIlvfmG|aX;uJJRzcWepZYLl[EBjgSCHR1dCpC=> MXyyN|I3OjJyOB?=
CT26 NHm4OmNHfW6ldHnvckBCe3OjeR?= MlixNlAhdU1? Moe2NUBp NV;l[IRme3WycILld5NmeyCKR1[tbY5lfWOnZDDWSWdHKGW6cILld5Nqd28EoB?= NYfJd3h[OjN{M{OxOlM>
UM-SCC-17B NVnOeplOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTJwNU[yJOKyKDBwNEC5JO69VSxiR1m1NF0yNjJ5OTFCtUAxNjF7NDFOwG0> NIjESG0zOjd5MEi5PS=>
OSC-19 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlTLTWM2OD1|LkS4NUDDuSByLkm1N{DPxE1uIFfJOVA:OS5|Nk[gxtEhOC55N{Cg{txO NYDaZYE2OjJ5N{C4PVk>
Cal33 NIr4ZmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3H4V2lEPTB;Mj6yPFIhyrFiMD60NlMh|ryPLDDHTVUxRTFwM{S5JOKyKDBwM{[zJO69VQ>? MlzaNlI4PzB6OUm=
UM-SCC-22B MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWL1fohtUUN3ME2yMlY1QCEEsTCwMlU1OiEQvF2sJGdKPTB;MT6zNlAhyrFiMD6yNFQh|ryP NE\DXI4zOjd5MEi5PS=>
UM-SCC-17B NGHkToFHfW6ldHnvckBCe3OjeR?= NV3Dc|NTOC1|MDFOwG0> MUiwMVI1KGh? MV7pcohq[mm2czDTWGFVOyCjY4TpeoF1cW:wIHTvd4Uh[W6mIITpcYUh\GWyZX7k[Y51dHl? MnjSNlI4PzB6OUm=
OSC-19 MYfGeY5kfGmxbjDBd5NigQ>? NEjEdoQxNTNyIN88US=> MkPONE0zPCCq NYj4T|BncW6qaXLpeJMhW1SDVEOgZYN1cX[jdHnvckBld3OnIHHu[EB1cW2nIHTldIVv\GWwdHz5 NFLGVVkzOjd5MEi5PS=>
Cal33 NYTMV4x7TnWwY4Tpc44hSXO|YYm= NYLYfG42OC1|MDFOwG0> NF65T4YxNTJ2IHi= NHfsbmlqdmirYnn0d{BUXEGWMzDhZ5RqfmG2aX;uJIRwe2ViYX7kJJRqdWViZHXw[Y5l\W62bIm= MV6yNlc4ODh7OR?=
UM-SCC-22B NUfXRm86TnWwY4Tpc44hSXO|YYm= MYKwMVMxKM7:TR?= MXqwMVI1KGh? MkfXbY5pcWKrdIOgV3RCXDNiYXP0bZZifGmxbjDkc5NmKGGwZDD0bY1mKGSncHXu[IVvfGy7 NXHjPFdNOjJ5N{C4PVk>
U-87MG MXnD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NWXnOJhvOC1zMDFOwG0> MnzlO|IhcA>? MlzHSG1UVw>? Mlm2bY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? NWi5b3M{OjV2M{[2PFI>
U-373MG MnXhR4VtdCCYaXHibYxqfHliQYPzZZk> MWSwMVExKM7:TR?= NIX2RpM4OiCq MWDEUXNQ NFfF[2pqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 M4\oZlI2PDN4Nkiy
SH-SY5Y M134fGNmdGxiVnnhZoltcXS7IFHzd4F6 MVewMVExKM7:TR?= MmXXO|IhcA>? NYDSXIZuTE2VTx?= NGHBeVZqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 MUiyOVQ{PjZ6Mh?=
Tu-9648 NYn3XZd[S2WubDDWbYFjcWyrdImgRZN{[Xl? MVWwMVExKM7:TR?= MmPYO|IhcA>? MYHEUXNQ NWXnVFZEcW6qaXLpeJMh[2WubDD2bYFjcWyrdImg[I9{\SCmZYDlcoRmdnSueR?= M4XFb|I2PDN4Nkiy
Neuro-2a MnHrR4VtdCCYaXHibYxqfHliQYPzZZk> NUHh[JdqOC1zMDFOwG0> MljrO|IhcA>? MnLDSG1UVw>? M1P2PIlvcGmkaYTzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? MmPzNlU1OzZ4OEK=
PCNs MVfD[YxtKF[rYXLpcIl1gSCDc4PhfS=> Mnm1NE0yOCEQvF2= NIjFRYE4OiCq Mn;TSG1UVw>? NEnJZ|hqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 NYXwT3JvOjV2M{[2PFI>
PGCs NVHjcnJpS2WubDDWbYFjcWyrdImgRZN{[Xl? MWewMVExKM7:TR?= MUW3NkBp NH;ZbpJFVVOR NYPiOY97cW6qaXLpeJMh[2WubDD2bYFjcWyrdImg[I9{\SCmZYDlcoRmdnSueR?= NV\zcYFxOjV2M{[2PFI>
RAW264.7 Mn\USpVv[3Srb36gRZN{[Xl? MV[xNEDPxE1? M12y[FEzKGh? Mn7oZYJzd2ejdHXzJJRp\SCvUl7BJIV5eHKnc4Ppc45{KG:oIFrBT|ItKFOWQWSxMEBUXEGWMjygZY5lKFOWQWSzJIlv\HWlZXSgZpkhTE:QIHHu[EBVNTJidH;4bY4> Mm\ZNlI1PTR2M{G=
RAW264.7 M33zOmFxd3C2b4Ppd{BCe3OjeR?= M2Ky[lUwOTBizszN MknPOFUhdWmw NYK1XHJ3\W6qYX7j[ZMhfG:6aX7zJIlv\HWlZXSgZZBweHSxc3nzJIFv\CCPTWCgcI9{ew>? MWSyNlQ2PDR|MR?=
SW480 Mn:zR4VtdCCYaXHibYxqfHliQYPzZZk> NE\SVZc2NzFyL{KwJO69VQ>? MkL4O|IhcA>? MoDxbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHlib3[geIhmKEGORFirM2NFOTN|K9MgZ4VtdHN? MWWyNVkxODN7Nx?=
HCT116 NV3ZXI1rS2WubDDWbYFjcWyrdImgRZN{[Xl? MVq1M|ExNzJyIN88US=> MWm3NkBp MmrBbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHlib3[geIhmKEGORFirM2NFOTN|K9MgZ4VtdHN? MmPsNlE6ODB|OUe=
DLD-1  NGOwbVhE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MYC1M|ExNzJyIN88US=> Ml\QO|IhcA>? MU\pcohq[mm2czDj[YxtKH[rYXLpcIl1gSCxZjD0bIUhSUyGSDuvR2QyOzNtwrDj[Yxtew>? NIT6V|gzOTlyMEO5Oy=>
SNU387  MoT6R4VtdCCYaXHibYxqfHliQYPzZZk> MlLoNlDDqM7:TdMg MnK2NlQhcA>? M{jOcJJm\HWlZYOgZ4VtdCC4aXHibYxqfHl? M{T3OFIyOzFzOUe1
SNU398 M1PpUGNmdGxiVnnhZoltcXS7IFHzd4F6 NHPYOmozOMLizszNxsA> M2nkbFI1KGh? MXjy[YR2[2W|IHPlcIwhfmmjYnnsbZR6 MWKyNVMyOTl5NR?=
HepG2 Mn7KR4VtdCCYaXHibYxqfHliQYPzZZk> NHG1S3ozOMLizszNxsA> NIrFNmkzPCCq MVny[YR2[2W|IHPlcIwhfmmjYnnsbZR6 NEXJVWEzOTNzMUm3OS=>
Huh-7 NHe2Z2RE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NXXhNVB3OjEEoN88UeKh MknnNlQhcA>? MljodoVlfWOnczDj[YxtKH[rYXLpcIl1gQ>? NFqwOWQzOTNzMUm3OS=>
VSMC NHvVOFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4XOR|MwPS9zMDFOwG0> M3WxN|MxKG2rbh?= MknISG1UVw>? MYjwdoV3\W62czDQSGdHNSCjbnSgeIhzd22kaX6tcYVlcWG2ZXSgWnNOSyCycn;sbYZmemG2aX;uJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MYGyNFg1PzNyNh?=
MDA-MB-231 M{fVfGFxd3C2b4Ppd{BCe3OjeR?= M4\vUFExKM7:TR?= MljvNlQhcA>? NH2yPXNFVVORwrC= NUmxU|lUcW6mdXPld{BieG:ydH;zbZM> NF7nOWEyPzFzNECwOS=>
MDA-MB-435S NXL5dotlSXCxcITvd4l{KEG|c3H5 M1HZeFExKM7:TR?= M1TmTlI1KGh? NU[zdIFETE2VT9Mg M4Xad4lv\HWlZYOgZZBweHSxc3nz NXTkU3BwOTdzMUSwNFU>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-STAT3 / STAT3 ; 

PubMed: 25261365     


(A) PC3M-1E8 cells were treated with different doses of Stattic for 4 h. (B) PC3M-1E8 cells were treated with 10 μM Stattic for different time points. 

Survivin / c-Myc / Bcl-xl ; 

PubMed: 25261365     


Western blot analysis of STAT3 downstream targets in PC3M-1E8 cells treated with 10 μM Stattic for 24 h. 

PARP / C-PARP / Caspase-3 / C-Caspse-3 ; 

PubMed: 23382914     


NPC cells were exposed to the indicated concentrations of Stattic for 48 h; apoptotic cells were measured by western blot analysis of cleaved PARP and cleaved caspase-3. Protein levels were quantified using ImageJ software. 

25261365 23382914
Immunofluorescence
p-STAT3 / STAT3 / Survivin ; 

PubMed: 25261365     


Cellular localization of pSTAT3 and survivin (green) was observed by confocal microscopy after immunofluorescent staining of PC3M-1E8 cancer cells treated with 10 μM Stattic.

25261365
Growth inhibition assay
Cell viability; 

PubMed: 23382914     


Stattic inhibits the cell viability of NPC cells in a time-dependent manner. CNE2 cells were treated with 8 µM Stattic for the indicated times, and cell viability was measured by the MTT assay. 

23382914
ELISA
BDNF; 

PubMed: 27456333     


BDNF secretion was analyzed using ELISA in A549 and H1299 cells treated with or without Stattic. Results are shown as the mean ± S.E. n = 3; *p < 0.05, vs Control group, Student’s t-test. 

27456333

Protocol

Kinase Assay:

[1]

- Collapse

High-Throughput Screening and FluorescencePolarization Assays:

Screening is performed at approximately 30 °C. The specificity ofscreening hits is validated in analogous assays for binding of thetest compounds to the SH2 domains of STAT1, STAT5, and Lck. The final concentration of buffer components used for all FP assays is 10 mM HEPES (pH 7.5), 1 mM EDTA, 0.1% Nonidet P-40, 50 mM NaCl, and 10% DMSO. The absence of dithiothreitol is essential for inhibitory activity. The sequences of the peptides are: STAT3, 5-carboxyfluorescein-GY(PO3H2)LPQTV-NH2; STAT1, 5-carboxyfluorescein-GY(PO3H2)DKPHVL; STAT5, 5-carboxyfluorescein-GY(PO3H2)LVLDKW; and Lck, 5-carboxyfluorescein-GY(PO3H2)EEIP. For specificity analysis at 30 °C, proteins are used at 150 nM (STAT1, STAT3, and STAT5). For specificity analysis at 37 °C, proteins are used at 370 nM (STAT3) or 100 nM (Lck). Proteins are incubated with test compounds in Eppendorf tubes at the indicated temperatures for 60 min prior addition of therespective 5-carboxyfluorescein labeled peptides (final concentration: 10 nM).Before measurement at room temperature, the mixtures are allowed to equilibrate for at least 30 min. Test compounds are used at the indicated concentrations diluted from a 20× stock in DMSO. Binding curves and inhibition curves are fitted with SigmaPlot. All competition curves are repeated three times in independent experiment
Cell Research:

[2]

- Collapse
  • Cell lines: Ly3 cells
  • Concentrations: ~2.5 μM
  • Incubation Time: 48 h
  • Method:

    MTS


    (Only for Reference)
Animal Research:

[2]

- Collapse
  • Animal Models: Ly3 parental NOD/SCID IL2R null xenograft
  • Dosages: 3.75 mg/kg every day
  • Administration: intra tumor
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 42 mg/mL (198.87 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O
For best results, use promptly after mixing.
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 211.19
Formula

C8H5NO4S

CAS No. 19983-44-9
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC(=CC2=C1C=CS2(=O)=O)[N+](=O)[O-]

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    We need to dissolve the compound for i.p. injection, could you give me some suggestions?

  • Answer:

    S7024 Stattic can be dissolved in 2% DMSO/PEG 300/dd H2O at 8 mg/mL as a clear solution.

STAT Signaling Pathway Map

Related STAT Products

Tags: buy Stattic | Stattic supplier | purchase Stattic | Stattic cost | Stattic manufacturer | order Stattic | Stattic distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID