C188-9

Catalog No.S8605

C188-9 Chemical Structure

Molecular Weight(MW): 471.52

C188-9 is a potent inhibitor of STAT3 that binds to STAT3 with high affinity (KD=4.7±0.4 nM). C188-9 is well tolerated in mice, shows good oral bioavailability, and is concentrated in tumors.

Size Price Stock Quantity  
USD 127 In stock
USD 390 In stock
USD 890 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Cited by 2 Publications

1 Customer Review

  • (E) Hep3B cells were pre-infected with flag-CEP55 for 24 h and were then infected with 10 nM JAK2 inhibitor XL019 or 10 nM STAT3 inhibitor C188-9 for 48 h. Cells’ invasion ability was determined by trans-well assays. Representative microscope images are plotted. Bars, 50 μm;

    Cells, 2018, 7(8). C188-9 purchased from Selleck.

Purity & Quality Control

Choose Selective STAT Inhibitors

Biological Activity

Description C188-9 is a potent inhibitor of STAT3 that binds to STAT3 with high affinity (KD=4.7±0.4 nM). C188-9 is well tolerated in mice, shows good oral bioavailability, and is concentrated in tumors.
Targets
STAT3 [1]
(Cell-free)
4.7 nM(Kd)
In vitro

C188-9 is a small-molecule inhibitor of Stat3 that targets the phosphotyrosyl peptide binding site within the Stat3 Src homology 2 (SH2) domain with Ki 136 nM. It does not inhibit upstream Jak or Src kinases[1].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Y79 M1mxOmZ2dmO2aX;uJIF{e2G7 MWKxNEDPxE1? MkPSNlg3QDR2MUm=
SK-N-DZ NHPOW3RHfW6ldHnvckBie3OjeR?= M3TFSlExKM7:TR?= M33SSlI5Pjh2NEG5
Huh7 MXPD[YxtKH[rYXLpcIl1gSCjc4PhfS=> MYm0PEBp NVLrXHB2UUN3ME2xNU4zPyEQvF2= MkHFNlg2OzN{MkW=
PLC/PRF/5 MmniR4VtdCC4aXHibYxqfHliYYPzZZk> Mk\BOFghcA>? MmizTWM2OD1zMT64N{DPxE1? NY\Nc48yOjh3M{OyNlU>
HepG2 M1\IemNmdGxidnnhZoltcXS7IHHzd4F6 MYK0PEBp M{jMOGlEPTB;MUCuNVkh|ryP NInsOJUzQDV|M{KyOS=>
SCC-35 NWPEOlJyS2WubDDndo94fGhiYYPzZZk> MX6wM|AvOS9yLkOvNU8{NzFyL{GwNEDPxE1? MnrZN|YhcA>? NF7nSmFKSzVyPUGwMlghyrFiMD6wJO69VQ>? MV6yO|AzPzR2NR?=
SCC-61 NHHnZYdE\WyuIHfyc5d1cCCjc4PhfS=> M{PxZ|AwOC5zL{CuN{8yNzNxMUCvNVAxKM7:TR?= NYD6N2F{OzZiaB?= MYLJR|UxRTF2LkigxtEzNjlizszN MmnjNlcxOjd2NEW=
UM-SCC-17B NVPhcZR4S2WubDDndo94fGhiYYPzZZk> Ml\6NE8xNjFxMD6zM|EwOy9zMD:xNFAh|ryP NXLOTnV[OzZiaB?= MVfJR|UxRTBwNzFCtUAxNjZizszN MV6yO|AzPzR2NR?=
HN30 NFnLdnlE\WyuIHfyc5d1cCCjc4PhfS=> M3z0R|AwOC5zL{CuN{8yNzNxMUCvNVAxKM7:TR?= MXqzOkBp MUDJR|UxRTRwNDFCtUAxNjFizszN NF7mOXozPzB{N{S0OS=>
A549 MVfGeY5kfGmxbjDhd5NigQ>? NXz1U5F2Oi53IHi= NVe1TWFs\GWlcnXhd4VlKHCVVFHUN{Bt\X[nbIOgbY4h[SClb37j[Y51emG2aX;uMYRmeGWwZHXueEBu[W6wZYKgd5RienSrbnegZZQh[2:wY3XueJJifGmxboOgZZMhdG:5IHHzJFMhdk1? MmLaNlY1OTBzN{e=
Kasumi-1 M{fMUWZ2dmO2aX;uJIF{e2G7 M3n6bmlEPTB;ND61JO69VQ>? M3LIVVIyPDR5OEOw
THP-1 NHHFVI1HfW6ldHnvckBie3OjeR?= NVriU5FQUUN3ME20Mlgh|ryP MXqyNVQ1Pzh|MB?=
GDM-1 NG\3SlRHfW6ldHnvckBie3OjeR?= NYTycpJTUUN3ME21MlAh|ryP MoXSNlE1PDd6M{C=
NB-1 M2npSGZ2dmO2aX;uJIF{e2G7 MX;JR|UxRThwMzFOwG0> MlzKNlE1PDd6M{C=
HL-60 NITsfHpHfW6ldHnvckBie3OjeR?= MYPJR|UxRTVwMzFOwG0> Mnj2NlE1PDd6M{C=
KG-1 NGrQeIxHfW6ldHnvckBie3OjeR?= NILmdlNKSzVyPUSuNUDPxE1? M1vYU|IyPDR5OEOw
K562 NGHp[nRCeG:ydH;zbZMh[XO|YYm= MV;pcoR2[3Srb36gc4Yh[XCxcITvd4l{KHerdHigZY4hTUN3MDDv[kA1Oy54IN88US=> MV6yNVQ1Pzh|MB?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-STAT3 / STAT3 / CEBPδ / Myostatin / p-Akt / Akt ; 

PubMed: 24011072     


C2C12 myotubes were treated with or without C188-9 for 2 h before adding IL-6 (100 ng/ml) for 24 h. A representative western blot for the indicated proteins is shown.

24011072
Immunofluorescence
VE-cadherin / β-catenin; 

PubMed: 26948077     


Confocal microscopy of VE-cadherin (green), total β-catenin (red), and DAPI (blue) in DMSO and C188–9(1µM) pre-treated HUVECs then subsequently exposed to histamine (100 µM). 

non-phosphorylated β-Catenin; 

PubMed: 26948077     


Confocal microscopy of active (non-phosphorylated) β-Catenin (green) and DAPI (blue) in DMSO and C188–9 (1 µM) pre-treated HUVECs then subsequently exposed to histamine (100 µM). 

26948077
In vivo Treatment of nude mice bearing xenografts of UM-SCC-17B, a radioresistant HNSCC line, with C188-9, but not C188, prevented tumor xenograft growth. C188-9 was well tolerated in mice, showed good oral bioavailability, and was concentrated in tumors[2].

Protocol

Cell Research:

[2]

- Collapse
  • Cell lines: HNSCC cell line, UM-SCC-17B
  • Concentrations: 0, 0.1, 0.3, 1, 3, 10, 30 μM
  • Incubation Time: 24 h
  • Method:

    UM-SCC-17B cells were incubated with various doses (0/0.1/0.3/1/3/10/30μM) of C188 or C188-9 for 24 hrs.


    (Only for Reference)
Animal Research:

[1]

- Collapse
  • Animal Models: CD2F1 female mice received isogenic C26 tumor cell via s.c injection
  • Formulation: D5W (5g of dextrose in 100 ml of water)
  • Dosages: 12.5 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 94 mg/mL (199.35 mM)
Ethanol 6 mg/mL (12.72 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 471.52
Formula

C27H21NO5S

CAS No. 432001-19-9
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

STAT Signaling Pathway Map

Related STAT Products

Tags: buy C188-9 | C188-9 supplier | purchase C188-9 | C188-9 cost | C188-9 manufacturer | order C188-9 | C188-9 distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID