Catalog No.S2285

Cryptotanshinone Chemical Structure

Molecular Weight(MW): 296.36

Cryptotanshinone is a STAT3 inhibitor with IC50 of 4.6 μM in a cell-free assay, strongly inhibits phosphorylation of STAT3 Tyr705, with a small effect on STAT3 Ser727, but none against STAT1 nor STAT5.

Size Price Stock Quantity  
USD 50 In stock
USD 110 In stock
USD 210 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Cited by 18 Publications

6 Customer Reviews

  • (a) Effect on STAT3 DNA binding activity of the STAT3 inhibitors cryptotanshinone (CTN) and S31-201 in STAT3 mutant cell lines OCI-Ly12 and OCI-Ly13.2. (b) Effect on cell viability at 48 h of the STAT3 inhibitors cryptotanshinone and S31-201 in OCI-Ly12 and OCI-Ly13.2 cells.

    Nat Commun, 2017, 8:14290. Cryptotanshinone purchased from Selleck.

  • After treatment with STAT3 inhibitor cryptotanshinone (crypto; 20 μmol/L) for 48 h, ADSCs were detected for the expression of p-STAT3, STAT3, and exosomal markers. Macrophages were stimulated with exosomes from ADSCs treated with crypto; the expression of Arg-1, p-STAT3, and STAT3 was detected by Western blot

    Diabetes, 2018, 67(2):235-247. Cryptotanshinone purchased from Selleck.

  • Immunoblot analysis of STAT3 in PC3 cells treated or not with cryptotanshinone at the indicated concentration for 30 min or DMSO as a control before incubation in culture medium.

    Cancer Lett, 2018, 416:24-30. Cryptotanshinone purchased from Selleck.

  • (E, F) The mPAC-MIP-RFP cells were infected with Ad-PNM, and treated with cryptotanshinone (CPT; 1 μM), BP-1-102 (10 μM), or DMSO. The percentage of RFP-positive cells among GFP-expressing cells was quantified. *, p < .05 versus DMSO.

    EBioMedicine, 2018, 36:358-366. Cryptotanshinone purchased from Selleck.

  • a. Changes in the expression of p-STAT3, p-ERK1/2, p-AKT, STAT3, p-ERK1/2 and AKT according to Western blotting. p-STAT3 and p-ERK1/2 were detected during the first 30 min of rhIL-6 treatment, indicating that rhIL-6 activated the JAK/STAT3 and MAPK/ERK1/2 pathways but not the PI3K/AKT pathway. b. MTT assay for F5M2 cells treated with different concentrations of the STAT3 inhibitor cryptotanshinone or the ERK1/2 inhibitor FR180204. Both inhibitors reduced F5M2 cell proliferation compared with the control DMSO (P < 0.001). Cryptotanshinone had a substantially greater inhibitory effect than FR180204 (P < 0.001). ED50cryptotanshinone=19.03 μM and ED50FR180204=89.20 μM. c. Pre-treatment of F5M2 cells with 50 μM cryptotanshinone prior to rhIL-6 incubation effectively reduced p-STAT3 levels. d. Pre-treatment of F5M2 cells with 2 μM FR180204 prior to rhIL-6 incubation effectively reduced p-ERK1/2 expression.

    Oncotarget, 2016, 7(1):446-58. Cryptotanshinone purchased from Selleck.

  • A. A2780 and ES2 cells were treated with different concentrations of STAT3 inhibitor, Cryptotanshinone (0, 0.5, 1, 1.5 μM for A2780 cells and 0, 20, 40, 60 μM for ES2 cells) for 24 h. Then the expression level of p-STAT3, STAT3, Bcl-2 and Survivin were analyzed by Western blotting.

    Biochem Biophys Res Commun, 2016, 470(4):947-54.. Cryptotanshinone purchased from Selleck.

Purity & Quality Control

Choose Selective STAT Inhibitors

Biological Activity

Description Cryptotanshinone is a STAT3 inhibitor with IC50 of 4.6 μM in a cell-free assay, strongly inhibits phosphorylation of STAT3 Tyr705, with a small effect on STAT3 Ser727, but none against STAT1 nor STAT5.
STAT3 [1]
(HCT-116 cells)
4.6 μM
In vitro

Cryptotanshinone, a natural compound isolated from the roots of Salvia miltiorrhiza Bunge (Danshen), significantly inhibits STAT3-dependent luciferase activity, the STAT3 Tyr705 phosphorylation and the dimerization of STAT3, compared to tanshinone IIA which exhibits no activity. Cryptotanshinone (7 μM) dramatically blocks STAT3 Tyr705 phosphorylation but not STAT3 Ser727 phosphorylation in DU145 cells, and significantly inhibits JAK2 phosphorylation with IC50 of ~5 μM without affecting the phosphorylation of upstream kinases c-Src and EGFR, suggesting the inhibition of STAT3 Tyr705 phosphorylation might due to a direct mechanism probably by binding to the SH2 domain of STAT3. Cryptotanshinone significantly inhibits the proliferation of DU145 prostate cancer cells harboring constitutively active STAT3 with GI50 of 7 μM by blocking STAT3 activity, which leads to the down-regulation of cyclin D1, Bcl-xL, and survivin, subsequently the accumulation in the G0-G1 phase. Cryptotanshinone exhibits less growth inhibitory effect on PC3, LNCaP and MDA-MB-468 cells. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human Hep3B cells MVXGeY5kfGmxbjDhd5NigQ>? NX;JbXhQOTZiaB?= NI\weJNKdmirYnn0bY9vKG:oIFjJSlEh[WO2aY\heIlwdiCrbjDoeY1idiCKZYCzRkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHj5dI95cWFvaX7keYNm\CCudXPp[oVz[XOnIHX4dJJme3Orb36gZYZ1\XJiMU[gbJJ{KGK7IILldI9zfGW{IHHzd4F6NCCLQ{WwQVEvOzZizszN MoP2NVc2QDN7NUC=
human AGS cells MWnGeY5kfGmxbjDhd5NigQ>? NIO4SVUyPiCq NYDvVWo1UW6qaXLpeIlwdiCxZjDITWYyKGGldHn2ZZRqd25iaX6gbJVu[W5iQVfTJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiaInwc5hq[S2rbnT1Z4VlKGy3Y3nm[ZJie2ViZYjwdoV{e2mxbjDh[pRmeiBzNjDodpMh[nlicnXwc5J1\XJiYYPzZZktKEmFNUC9NU42QCEQvF2= NVHBPJRqOTd3OEO5OVA>
human HCT116 cells NEHje2VHfW6ldHnvckBie3OjeR?= MnTRNlQhcA>? M362[mlvcGmkaYTpc44hd2ZiU2TBWFMh\XiycnXzd4VlKGmwIHj1cYFvKEiFVEGxOkBk\WyuczDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBq[zVyPUSuOkDPxE1? NH;ufmkzOjZ3MEOyOS=>
human MIAPaCa2 cells MWDDfZRwfG:6aXRCpIF{e2G7 NV36dXFNOjRiaB?= MXPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNTWFR[UOjMjDj[YxteyCjZoTldkAzPCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVUvQCEQvF2= NFnF[mozOTd5NUG1Oi=>
human KB-3-1 cells NEPOOItEgXSxdH;4bYPDqGG|c3H5 MmjmOFghcA>? NIPNV45EgXSxdH;4bYNqfHliYXfhbY5{fCCmcoXnMZNmdnOrdHn2[UBpfW2jbjDLRk0{NTFiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VWy:STWOgZZN{[XluIFnDOVA:Pi57IN88US=> MUOxNVczODV{MB?=
human KBV1 cells Mn\KR5l1d3SxeHnjxsBie3OjeR?= MUe0PEBp M125W2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KG23bITp[JJ2\y2{ZYPpd5RidnRiaIXtZY4hU0KYMTDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSVL2DNV{Bie3OjeTygTWM2OD15LkWg{txO M{\6blEyPzJyNUKw
human AGS cells MVPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWeyOEBp NVLCcFFPXmmjYnnsbZR6KG:oIHj1cYFvKEGJUzDj[YxteyC3bnTldkBpgXCxeHnjJINwdmSrdHnvcpMh[W[2ZYKgNlQhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xNE4zKM7:TR?= M33TeFE4PTh|OUWw
human THLE3 cells NUTF[ZBTS3m2b4TvfIlkyqCjc4PhfS=> MYfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDUTGxGOyClZXzsd{BjgSCPVGSgZZN{[XluIFXDOVA:OjJwOTFOwG0> NXzkS5kyOjB2NUW1O|g>
human PBMC MYPGeY5kfGmxbjDhd5NigQ>? MYDJcohq[mm2aX;uJI9nKHCqeYTvbIVu[WepbIX0bY5qdmmwIFGtd5RqdXWuYYTl[EBpfW2jbjDQRm1EKHC{b3zp[oVz[XSrb36gZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5iW{G0R311cHmvaXTpcoUhcW6lb4Lwc5JifGmxbjygTWM2OD1|Nz60JO69VQ>? MmTUNVE4OjB3MkC=
PTPN11 E76K/+ MEF M4XvfWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MojuNlAh|ryP MXvHdo94fGhiaX7obYJqfGmxbjDv[kBRXFCQMUGgSVc3Uy9tIF3FSkBifCB{MDD1US=> NUjzXlhJOjN7NUe0NlY>
human HeLa cells NYWzTWhrT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWCyOEBp MnTUS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gTIVN[SClZXzsd{Bi\nSncjCyOEBpenN? MYKyN|k2PzR{Nh?=
human HepG2 cells NYnIXpNWS3m2b4TvfIlkyqCjc4PhfS=> MkGzR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMh[nliTWTUJIF{e2G7 M4DJW|E3ODN6NUWw
human OVCAR-3 cells M3W2c2N6fG:2b4jpZ:Kh[XO|YYm= NHjGW3ZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBQXkODUj2zJINmdGy|IHL5JG1VXCCjc4PhfS=> MXKxOlA{QDV3MB?=
mouse Ba/F3 cells Mn;lSpVv[3Srb36gZZN{[Xl? MYWzNEDPxE1? NYXxXIV2OyCq NVziTGJ6UW6qaXLpeIlwdiCxZjDJUFMucW6mdXPl[EBUUFB{LVfhZlIhcW62ZYLhZ5Rqd25iaX6gcY92e2ViQnGvSlMh[2WubIOgdJJmfHKnYYTl[EBifCB|MDD1UUBnd3JiMzDodpMheHKrb4KgTWw{KHO2aX31cIF1cW:wIHL5JIludXWwb3Lsc5R1cW6pIH3leIhw\A>? Ml3ENlM6PTd2Mk[=

... Click to View More Cell Line Experimental Data

In vivo Cryptotanshinone administration significantly reduces the body weight and food intake of ob/ob mice (C57BL/6J-Lepob) and diet-induced obese (DIO) mice in a dose-dependent manner. Cryptotanshinone causes noticeably less fat in the adipose tissues, significant reductions of serum triglycerides and cholesterol levels, and 2.5- to 3-fold higher AMPK activity of the skeletal muscles than in the control mice. Oral administration of Cryptotanshinone at 600 mg/kg/day produces dramatic reductions in blood glucose levels of ob/ob mice (C57BL/6J-Lepob), db/db mice (C57BL/KsJ-Leprdb), and ZDF rats, which occur after 3 days and persist over the entirety of the monitoring period. [2]


Kinase Assay:[1]
+ Expand

STAT3-dependent dual-luciferase assay:

HCT-116 cells are transiently transfected with reporter plasmid having the STAT3-binding element for regulating luciferase assay. Cells are treated with Cryptotanshinone for 24 hours at a concentration range of 0.2 to 50 μM. After treatment, cells are harvested in 20 μL of passive lysis buffer and luciferase activity is evaluated by the Dual Luciferase Reporter Assay kit on Wallac Victor2. The concentration of Cryptotanshinone that inhibits the luciferase activity by 50% represents IC50 value.
Cell Research:[1]
+ Expand
  • Cell lines: KATO III, DU145, PC3, LNCaP, MDA-MB-231, MDA-MB-468, MDA-MB-453, MCF-7, MCF-10A, HeLa and HCT-116
  • Concentrations: Dissolved in DMSO, final concentrations ~50 μM
  • Incubation Time: 24 or 48 hours
  • Method: Cells are exposed to Cryptotanshinone for 24 or 48 hours. For the determination of cell proliferation, the cell proliferation reagent WST-1 is added and WST-1 formazan is quantitatively measured at 450 nm using an ELISA reader.
    (Only for Reference)
Animal Research:[2]
+ Expand
  • Animal Models: Zucker Diabetic Fatty (ZDF) (male) type 2 diabetic rat, ob/ob mice (C57BL/6J-Lepob), db/db mice (C57BL/KsJ-Leprdb) and male C57BL/6J mice with high-fat diet-induced obesity
  • Formulation: Dissolved in 0.1% solution of sodium lauryl sulfate
  • Dosages: ~600 mg/kg/day
  • Administration: Orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 5 mg/mL warmed (16.87 mM)
Ethanol 1 mg/mL warmed (3.37 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 296.36


CAS No. 35825-57-1
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    I want to use this compound for my mouse in vivo experiment, so how to reconstitute it?

  • Answer:

    Cryptotanshinone can be dissolved in 2% DMSO/30% PEG 300/5% Tween 80/ddH2O at 2 mg/ml. But after stayed for about 0.5-1 hour, the precipitation went out. So if you are going to use this vehicle, it is recommended to be prepared just before use.

STAT Signaling Pathway Map

Related STAT Products0

Tags: buy Cryptotanshinone | Cryptotanshinone supplier | purchase Cryptotanshinone | Cryptotanshinone cost | Cryptotanshinone manufacturer | order Cryptotanshinone | Cryptotanshinone distributor
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID