Home JAK/STAT STAT inhibitor SH5-07 (SH-5-07) For research use only.

SH5-07 (SH-5-07)

SH5-07 is a robust hydroxamic acid-based STAT3 inhibitor, which induce antitumor cell effects in vitro and antitumor response in vivo against human glioma and breast cancer models.

SH5-07 (SH-5-07) Chemical Structure

SH5-07 (SH-5-07) Chemical Structure

CAS: 1456632-41-9

Selleck's SH5-07 (SH-5-07) has been cited by 1 publication

Purity & Quality Control

Batch: S792301 DMSO] 100 mg/mL] false] Ethanol] 100 mg/mL] false] Water] Insoluble] false Purity: >97%
97

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Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
30M cells Cytotoxicity assay Cytotoxicity against human 30M cells assessed as cell viability after 3 days by Alamar Blue assay, IC50=0.1 μM 24900612
127EF cells Cytotoxicity assay Cytotoxicity against human 127EF cells assessed as cell viability after 3 days by Alamar Blue assay, IC50=0.1955 μM 24900612
67EF cells Cytotoxicity assay Cytotoxicity against human 67EF cells assessed as cell viability after 3 days by Alamar Blue assay, IC50=0.2145 μM 24900612
84EF cells Cytotoxicity assay Cytotoxicity against human 84EF cells assessed as cell viability after 3 days by Alamar Blue assay, IC50=0.295 μM 24900612
73EF cells Cytotoxicity assay Cytotoxicity against human 73EF cells assessed as cell viability after 3 days by Alamar Blue assay, IC50=0.8635 μM 24900612
25EF cells Cytotoxicity assay Cytotoxicity against human 25EF cells assessed as cell viability after 3 days by Alamar Blue assay, IC50=1.1205 μM 24900612
147EF cells Function assay 2 to 72 hrs Inhibition of STAT3 in human 147EF cells assessed as reduction of phosphorylated Bcl-xL level after 2 to 72 hrs by Western blotting analysis 24900612
Click to View More Cell Line Experimental Data

Biological Activity

Description SH5-07 is a robust hydroxamic acid-based STAT3 inhibitor, which induce antitumor cell effects in vitro and antitumor response in vivo against human glioma and breast cancer models.
Targets
STAT3 [1]
In vitro
In vitro SH5-07 blocks STAT3 DNA binding activity in vitro and in human glioma, breast, and prostate cancer cells and in v-Src-transformed murine fibroblasts. STAT3-dependent gene transcription is blocked along with Bcl-2, Bcl-xL, Mcl-1, Cyclin D1, c-Myc and Survivin expression[1].
Cell Research Cell lines Normal mouse fibroblasts (NIH3T3)
Concentrations 0-8 μM
Incubation Time 24-48 h
Method Cells are treated with 0-8 μM agent for 24-48 h. For cell cycle profile analysis, cells are harvested and fixed with 70% ice-cold ethanol and stained with propidium iodide (PI). For apoptosis analysis, cells are collected and stained with FITC-Annexin V using Apoptosis Detection Kit. Both the DNA content of cells and the Annexin V-positive cells are analyzed by FACScan flow cytometer. Cell cycle phase distribution is analyzed using the Cell-Fit program. Data acquisition is gated to exclude cell doublets.
In Vivo
In vivo In mouse xenograft models of glioma and breast cancer, administration of SH5-07 effectively inhibits tumor growth[1].
Animal Research Animal Models female athymic nude mice
Dosages 3, 5 or 6 mg/kg
Administration oral gavage/tail vein injection

Chemical Information & Solubility

Molecular Weight 625.61 Formula

C29H28F5N3O5S
CAS No. 1456632-41-9 SDF Download SH5-07 (SH-5-07) SDF
Smiles CN(CC(=O)N(CC1=CC=C(C=C1)C2CCCCC2)C3=CC=C(C=C3)C(=O)NO)S(=O)(=O)C4=C(C(=C(C(=C4F)F)F)F)F
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (159.84 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 100 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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