For research use only.
CAS No. 1172133-28-6
HO-3867, an analog of curcumin, is a selective STAT3 inhibitor that inhibits its phosphorylation, transcription, and DNA binding without affecting the expression of other active STATs. HO-3867 induces apoptosis.
Selleck's HO-3867 has been cited by 16 publications
4 Customer Reviews
Cell apoptosis as measured by TUNEL. Representative sections as determined at 12 hours after reperfusion (3100 magnification). Apoptotic nuclei were stained red, and the software of Image J was used to analyse quantity of TUNEL-positive cells in the livers. Scale bars5100 lm. *P<0.05, **P<0.01, ***P<0.001 compared with IR group.
Liver Transpl, 2016, 22(12):1697-1709. HO-3867 purchased from Selleck.
The inhibitory effect of IL-6/IL-6R on TRPM7 currents is blocked by the STAT3 inhibitor HO-3867, but not by the inhibitor MAPK signaling pathway. (A) Relative currents normalized to TRPM7 currents recorded during perfusion with divalent-free extracellular solution. The specific STAT3 inhibitor HO-3867 did not inhibit TRPM7 inward currents at -100 mV, but blocked the effect of IL-6/sIL-6R (n = 3, * p < 0.05 vs. control, # p < 0.05 vs. IL-6/sIL-6R). (B) The specific MAPK−MEK inhibitor PD98059, did not inhibit TRPM7 inward currents at -100 mV, and did not block the effect of IL-6/sIL-6R (n = 3, * p < 0.05 vs. control). All currents were normalized to controls at -100 mV.
PLOS ONE, 2016, 11(3): e0152120.. HO-3867 purchased from Selleck.
Inhibition of Stat3 activity on autophagy and hepatic IRI. (A) Pathological analyses showing that inhibition of Stat3 activity increased histopathologic injury in livers subjected to IRI treatment (×200). Scale bars=20μm. And quantified IR-induced liver injury by measuring Suzuki's score. (B) Levels of serum AST and ALT were increased when Stat3 activity was inhibited. (C) Quantitative analysis using TUNEL showing that inhibition of Stat3 activity increased hepatic apoptosis. (D) Immunohistochemistry showing that Cleaved caspase-3 expression increased when Stat3 was inhibited (magnification × 200). Scale bars=20μm. (E) Western blot analysis showing that ATG5 expression was decreased while Bax increased when the expression of p-stat3 (Tyr705) was decreased. (F) Quantitative analysis using transmission electron microscopy (TEM) showing that the number of autophagosomes per cross-sectioned cell were significantly decreased when Stat3 was inhibited. Each data point represents mean±standard deviation of three independent experiments. ***P < 0.001 vs 2h group.
J Cell Biochem, 2018, 119(4):3440-3450. HO-3867 purchased from Selleck.
Interleukin (IL)-22-mediated protection against sodium nitroprussiate (SNP)-induced apoptosis in fibroblast-like synoviocytes established from rheumatoid arthritis (RA) patients (RA-FLS) is mediated by upregulation of the anti-apoptotic proteins Bcl2 and Bcl-xL. Cells were pretreated with HO-3867 (10 lmol/L) or STA21 (25 lmol/L) 2 h before the addition of IL-22 (100 ng/mL). Cells were then cultured for 30 min prior to the addition of SNP (1.33 mmol/L) and incubation for an additional 24 h. a. Western blot analysis of Bcl-2 protein expression in RA-FLS. A representative Western blot is shown in the left panel. Expression of Bcl-2 was quantified by densitometric analysis (right panel). Data represent the mean SD of three independent experiments. b. Western blot analysis of Bcl-xL in RA-FLS. A representative Western blot is shown in the left panel. Expression of Bcl-xL was quantified by densitometric analysis (right panel). Data represent the mean SD of three independent experiments. Each value is expressed as the ratio of the measured protein level to that of b-actin. *P < 0.05, #P > 0.05 versus control, &P < 0.05 versus SNP, %P < 0.05 versus SNP+ IL-22.
Int J Rheum Dis, 2017, 20(2):214-224. HO-3867 purchased from Selleck.
Purity & Quality Control
Choose Selective STAT Inhibitors
|Description||HO-3867, an analog of curcumin, is a selective STAT3 inhibitor that inhibits its phosphorylation, transcription, and DNA binding without affecting the expression of other active STATs. HO-3867 induces apoptosis.|
HO-3867 produces significant cytotoxicity in A2780 and other tested ovarian cancer cell lines, with less toxic to noncancerous ovarian surface epithelial cells. HO-3867 induces G(2)-M cell cycle arrest in A2780 cells and promotes apoptosis by caspase-8 and caspase-3 activation. HO-3867 blocks the JAK/STAT3 pathway in human ovarian cancer cell lines. 
|In vivo||HO-3867 (100 ppm p.o.) inhibits the growth of ovarian cancer xenograft tumor in mice without any apparent signs of toxicity, and also results in inhibition of pSTAT3 as well as downregulation of the STAT3-targeting proteins.  HO-3867 sensitizes cisplatin-resistant ovarian carcinoma through STAT3 inhibition.  HO-3867 (100 ppm p.o.) also attenuates left-heart-failure-induced pulmonary hypertension by decreasing oxidative stress and increasing PTEN expression in the lung of rats. |
|In vitro||DMSO||13 mg/mL warmed (27.98 mM)|
|Ethanol||6 mg/mL warmed (12.91 mM)|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
In vivo Formulation Calculator (Clear solution)
|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
|Dosage||mg/kg||Average weight of animals||g||Dosing volume per animal||ul||Number of animals|
|Step 2: Enter the in vivo formulation ()|
|% DMSO % % Tween 80 % ddH2O|
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:
Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)
*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).
Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).
Molecular Weight Calculator
Enter the chemical formula of a compound to calculate its molar mass and elemental composition:
Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2
Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.