Aspirin

For research use only.

Catalog No.S3017 Synonyms: Acetylsalicylic acid

9 publications

Aspirin Chemical Structure

CAS No. 50-78-2

Aspirin (Acetylsalicylic acid) is a salicylate, and irreversible COX1 and COX2 inhibitor, used as an analgesic to relieve minor aches and pains, as an antipyretic to reduce fever, and as an anti-inflammatory medication. Aspirin induces autophagy and stimulates mitophagy.

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10mM (1mL in DMSO) USD 130 In stock
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Biological Activity

Description Aspirin (Acetylsalicylic acid) is a salicylate, and irreversible COX1 and COX2 inhibitor, used as an analgesic to relieve minor aches and pains, as an antipyretic to reduce fever, and as an anti-inflammatory medication. Aspirin induces autophagy and stimulates mitophagy.
Targets
COX2 [1] COX1 [1]
In vitro

Aspirin inhibits the activation of NF-kappa B, thus prevents the degradation of the NF-kappa B inhibitor, I kappa B, and therefore NF-kappa B is retained in the cytosol. Aspirin also inhibits NF-kappa B-dependent transcription from the Ig kappa enhancer and the human immunodeficiency virus (HIV) long terminal repeat (LTR) in transfected T cells. [1] Aspirin and salicylate are mediated in part by their specific inhibition of IKK-beta, thereby preventing activation by NF-kappaB of genes involved in the pathogenesis of the inflammatory response. [2] Aspirin is protective against neurotoxicity elicited by the excitatory amino acid glutamate in rat primary neuronal cultures and hippocampal slices. [3] Aspirin triggers transcellular biosynthesis of a previously unrecognized class of eicosanoidsduring coincubations of human umbilical vein endothelial cells (HUVEC) and neutrophils [polymorphonuclear leukocytes (PMN)]. Aspirin evokes a unique class of eicosanoids formed by acetylated PGHS-2 and 5-lipoxygenase interactions. [4] Aspirin treatment inhibits the phosphorylation of IRS-1 at Ser307 as well as the phosphorylation of JNK, c-Jun, and degradation of IkappaBalpha in 3T3-L1 and Hep G2 cells treated with tumor necrosis factor (TNF)-alpha. Aspirin treatment inhibits phosphorylation of Akt and the mammalian target of rapamycin (but not extracellular regulated kinase or PKCzeta) in response to TNF-alpha. Aspirin rescues insulin-induced glucose uptake in 3T3-L1 adipocytes pretreated with TNF-alpha. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human AGS cells M{L5fmZ2dmO2aX;uJIF{e2G7 M3PzdFE2NTZyIN88cY9tN0x? MWGxNkBp NUjBOXFsUW6qaXLpeIlwdiCxZjDFd4Np\XKrY3jpZUBkd2yrLYP0bY12dGG2ZXSgTWwuQCCycn;keYN1cW:wIHnuJIh2dWGwIFHHV{Bk\WyuczDheEAyPSC2bzC2NEB2dW:uL1ygZYZ1\XJiMUKgbJJ{KGK7IFXMTXNC NHjsT3gzODF3M{G4Ny=>
human PC3 cells M4PjTGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkKxN|AxKM7:TR?= NYnx[3VqPDhiaB?= MYnHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDQR|Mh[2WubIOgZZQhOzByIIXNJIFnfGW{IES4JIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeR?= M1TKSlIzPDl2NkG3
human HCT116 cells MWDGeY5kfGmxbjDhd5NigQ>? MV6xJI1O MVi2JIg> M4\4U2lvcGmkaYTpc44hd2ZiVF7GMYFteGijLXnu[JVk\WRiTl[tb4FxeGGEIHHjeIl3[XSrb36gbY4hcHWvYX6gTGNVOTF4IHPlcIx{KGG2IEGgcW0h[W[2ZYKgOkBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6 MYiyNlE2PDh|NB?=
human THP1 cells MVTGeY5kfGmxbjDhd5NigQ>? MonZNVAxKM7:TR?= MVmzNEBucW6| NYDKVItkUXK{ZY\ldpNq[mynIHnubIljcXSrb36gc4YhS0:[LUGgbY4hcHWvYX6gWGhROSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJIFz[WOqaXTvcolkKGGlaXStbY5lfWOnZDDUXGIzKG[xcn3heIlwdiCjdDCxNFAhfU1iaX7jeYJifGWmIH\vdkA{OCCvaX7zJIZwdGyxd3XkJIJ6KGOxbYDveY5lKHejc3jveZQhdWWjc4Xy[YQhOzBibXnud{Bxd3O2IHHyZYNpcWSxbnnjJIFkcWRiY3jhcIxmdmenIHL5JJJi\GmxaX3teY5w[XO|YYm= MnvHNlM3PTF|NUm=
human PANC1 cells M33RU2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWSzNFAh|ryP NGHERoc1QCCq NX\HSIhFT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hWEGQQ{GgZ4VtdHNiYYSgN|AxKHWPIHHmeIVzKDR6IHjyd{BjgSCjbHHtZZIh[my3ZTDhd5NigQ>? M4G5[lIzPDl2NkG3
human SKBR3 cells NILifGVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHvT[2g{ODBizszN MV60PEBp NEXOXVVIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBUU0KUMzDj[YxteyCjdDCzNFAhfU1iYX\0[ZIhPDhiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6 NX76bGpFOjJ2OUS2NVc>
human MDA-MB-231 cells M4TJS2Z2dmO2aX;uJIF{e2G7 M17odVExOCEQvF2= NFTp[YU{OCCvaX7z M{L4W2lzemW4ZYLzbYJt\SCrbnjpZol1cW:wIH;mJGNQYC1zIHnuJIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCjcnHjbIlld26rYzDhZ4llNWmwZIXj[YQhWEeHMjDmc5Ju[XSrb36gZZQhOTByIIXNJIlv[3WkYYTl[EBnd3JiM{CgcYlveyCob3zsc5dm\CCkeTDjc41xd3WwZDD3ZZNpd3W2IH3lZZN2emWmIEOwJI1qdnNicH;zeEBiemGlaHnkc45q[yCjY3nkJINp[WyuZX7n[UBjgSC{YXTpc4ludXWwb3Hzd4F6 M{XhRlI{PjVzM{W5

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
MCL-1; 

PubMed: 26918349     


Western blot analysis of the effects of aspirin on MCL-1 expression.

p-AKT / AKT / p-ERK / ERK ; 

PubMed: 26918349     


Western blot analysis of the effects of aspirin on Akt and ERK1/2 phosphorylation.

p-AMPKα / AMPKα / p-ACC / ACC; 

PubMed: 26918349     


Western blot analysis of the effects of aspirin on AMPK and ACC phosphorylation.

PubMed: 23741443     


Western blotting revealed that aspirin down-regulated COX1 and COX2 expression in HCC cell lines.

SHH / SMO / GLI1 / Bcl-2 / Foxm1 ; 

PubMed: 28446712     


Western blot analysis showed SHH, SMO, GLI1, Bcl-2 and Foxm1 proteins expression in U87 and T98G cells treated with indicated concentrations of aspirin for 24 h. GAPDH was used as an internal control. 

26918349 23741443 28446712
Growth inhibition assay
Cell proliferation ; 

PubMed: 28446712     


U87 and T98G cells were treated with indicated concentrations of aspirin and harvested after 24, 48 and 72 h. Cell proliferation was analyzed by CCK-8 assay. 

Cell viability; 

PubMed: 30221683     


The CCK-8 assay results are presented as bar graphs for the antiproliferative effects of aspirin on RA-FLS. (A-C) RA-FLS were treated with (0, DMSO, 1, 2, 5 and 10 mM) aspirin for 12, 24 and 48 h. At each time interval, cell viability was determined by CCK-8 analysis. Data are presented as the means ± SD (error bars) from three independent experiments. Aspirin inhibited the growth of RA-FLS in a dose-dependent manner. (D) Cell proliferation decreased at 12, 24 and 48 h. Data are presented as the means ± SD (error bars) from three independent experiments. *P<0.05, **P<0.01, ***P<0.001 vs. DMSO group. CCK-8: Cell-Counting Kit-8; RA-FLS, rheumatoid arthritis-fibroblast-like synoviocytes; DMSO, dimethyl sulfoxide; SD, standard deviation.

28446712 30221683

Protocol

Solubility (25°C)

In vitro DMSO 36 mg/mL (199.82 mM)
Water Insoluble
Ethanol ''36 mg/mL
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+PBS
For best results, use promptly after mixing.
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 180.16
Formula

C9H8O4

CAS No. 50-78-2
Storage powder
in solvent
Synonyms Acetylsalicylic acid
Smiles CC(=O)OC1=CC=CC=C1C(=O)O

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04356326 Not yet recruiting Drug: Aspirin 150 mg|Drug: Placebo Chronic Hypertension Complicating Pregnancy|Pre-Eclampsia|Intrauterine Growth Restriction|Aspirin|Perinatal Death|Placental Abruption Centre Hospitalier Intercommunal Creteil January 2021 Phase 3
NCT04308551 Not yet recruiting Drug: Indobufen|Drug: Aspirin Stable Coronary Heart Disease Henan Institute of Cardiovascular Epidemiology October 30 2020 Not Applicable
NCT03615846 Not yet recruiting -- Platelet Aggregation|Coronary Artery Disease Accriva Diagnostics October 20 2020 --
NCT04442256 Recruiting Drug: Dupilumab AERD - Aspirin Exacerbated Respiratory Disease Medical University of Vienna June 1 2020 Phase 4

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID