For research use only.

Catalog No.S3017 Synonyms: Acetylsalicylic acid

9 publications

Aspirin Chemical Structure

Molecular Weight(MW): 180.16

Aspirin is a salicylate, and irreversible COX1 and COX2 inhibitor, used as an analgesic to relieve minor aches and pains, as an antipyretic to reduce fever, and as an anti-inflammatory medication.

Size Price Stock Quantity  
10mM (1mL in DMSO) USD 130 In stock
USD 97 In stock
USD 470 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Purity & Quality Control

Choose Selective COX Inhibitors

Biological Activity

Description Aspirin is a salicylate, and irreversible COX1 and COX2 inhibitor, used as an analgesic to relieve minor aches and pains, as an antipyretic to reduce fever, and as an anti-inflammatory medication.
COX2 [1] COX1 [1]
In vitro

Aspirin inhibits the activation of NF-kappa B, thus prevents the degradation of the NF-kappa B inhibitor, I kappa B, and therefore NF-kappa B is retained in the cytosol. Aspirin also inhibits NF-kappa B-dependent transcription from the Ig kappa enhancer and the human immunodeficiency virus (HIV) long terminal repeat (LTR) in transfected T cells. [1] Aspirin and salicylate are mediated in part by their specific inhibition of IKK-beta, thereby preventing activation by NF-kappaB of genes involved in the pathogenesis of the inflammatory response. [2] Aspirin is protective against neurotoxicity elicited by the excitatory amino acid glutamate in rat primary neuronal cultures and hippocampal slices. [3] Aspirin triggers transcellular biosynthesis of a previously unrecognized class of eicosanoidsduring coincubations of human umbilical vein endothelial cells (HUVEC) and neutrophils [polymorphonuclear leukocytes (PMN)]. Aspirin evokes a unique class of eicosanoids formed by acetylated PGHS-2 and 5-lipoxygenase interactions. [4] Aspirin treatment inhibits the phosphorylation of IRS-1 at Ser307 as well as the phosphorylation of JNK, c-Jun, and degradation of IkappaBalpha in 3T3-L1 and Hep G2 cells treated with tumor necrosis factor (TNF)-alpha. Aspirin treatment inhibits phosphorylation of Akt and the mammalian target of rapamycin (but not extracellular regulated kinase or PKCzeta) in response to TNF-alpha. Aspirin rescues insulin-induced glucose uptake in 3T3-L1 adipocytes pretreated with TNF-alpha. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human AGS cells MlTESpVv[3Srb36gZZN{[Xl? M1jHd|E2NTZyIN88cY9tN0x? MYCxNkBp NEXIUFNKdmirYnn0bY9vKG:oIFXzZ4hmemmlaHnhJINwdGlvc4TpcZVt[XSnZDDJUE05KHC{b3T1Z5Rqd25iaX6gbJVu[W5iQVfTJINmdGy|IHH0JFE2KHSxIE[wJJVud2xxTDDh[pRmeiBzMjDodpMh[nliRVzJV2E> Mn7JNlAyPTNzOEO=
human PC3 cells M1TUeWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEPZPI8{ODBizszN NGXtSIY1QCCq NYG2co0xT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hWEN|IHPlcIx{KGG2IEOwNEB2VSCjZoTldkA1QCCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZk> NW[2eXduOjJ2OUS2NVc>
human HCT116 cells MWrGeY5kfGmxbjDhd5NigQ>? M2HoUlEhdU1? MWW2JIg> MW\Jcohq[mm2aX;uJI9nKFSQRj3hcJBp[S2rbnT1Z4VlKE6ILXvhdJBiSiCjY4TpeoF1cW:wIHnuJIh2dWGwIFjDWFEyPiClZXzsd{BifCBzIH3NJIFnfGW{IE[gbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=> NXGy[2dUOjJzNUS4N|Q>
human THP1 cells NW\3NHdLTnWwY4Tpc44h[XO|YYm= MYCxNFAh|ryP MUWzNEBucW6| Mlr5TZJz\X[ncoPpZoxmKGmwaHnibZRqd25ib3[gR29ZNTFiaX6gbJVu[W5iVFjQNUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHHyZYNpcWSxbnnjJIFkcWRvaX7keYNm\CCWWFKyJIZwem2jdHnvckBifCBzMECgeW0hcW6ldXLheIVlKG[xcjCzNEBucW6|IH\vcIxwf2WmIHL5JINwdXCxdX7kJJdie2ixdYSgcYVie3W{ZXSgN|AhdWmwczDwc5N1KGG{YXPobYRwdmmlIHHjbYQh[2ijbHzlcodmKGK7IILh[IlwcW2vdX7vZZN{[Xl? NX[3WIdLOjN4NUGzOVk>
human PANC1 cells M1HJ[mdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mlq0N|AxKM7:TR?= NH;2bmk1QCCq M3zZfmdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJHBCVkNzIHPlcIx{KGG2IEOwNEB2VSCjZoTldkA1QCCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZk> NFLLclkzOjR7NE[xOy=>
human SKBR3 cells NXHqSWZDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUPPNXRZOzByIN88US=> MVu0PEBp NVLZ[YRFT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hW0uEUkOgZ4VtdHNiYYSgN|AxKHWPIHHmeIVzKDR6IHjyd{BjgSCjbHHtZZIh[my3ZTDhd5NigQ>? NFnUSHEzOjR7NE[xOy=>
human MDA-MB-231 cells MnPaSpVv[3Srb36gZZN{[Xl? M2S1T|ExOCEQvF2= NH24PWc{OCCvaX7z MUDJdpJmfmW{c3nicIUhcW6qaXLpeIlwdiCxZjDDU3guOSCrbjDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yh[XKjY3jp[I9vcWNiYXPp[E1qdmS3Y3XkJHBITTJiZn;ycYF1cW:wIHH0JFExOCC3TTDpcoN2[mG2ZXSg[o9zKDNyIH3pcpMh\m:ubH;3[YQh[nliY3;tdI92dmRid3HzbI92fCCvZXHzeZJm\CB|MDDtbY5{KHCxc4SgZZJi[2irZH;ubYMh[WOrZDDjbIFtdGWwZ3WgZpkhemGmaX;pcY12dm:jc4PhfS=> M3\HSFI{PjVzM{W5

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot

PubMed: 26918349     

Western blot analysis of the effects of aspirin on MCL-1 expression.

p-AKT / AKT / p-ERK / ERK ; 

PubMed: 26918349     

Western blot analysis of the effects of aspirin on Akt and ERK1/2 phosphorylation.

p-AMPKα / AMPKα / p-ACC / ACC; 

PubMed: 26918349     

Western blot analysis of the effects of aspirin on AMPK and ACC phosphorylation.

PubMed: 23741443     

Western blotting revealed that aspirin down-regulated COX1 and COX2 expression in HCC cell lines.

SHH / SMO / GLI1 / Bcl-2 / Foxm1 ; 

PubMed: 28446712     

Western blot analysis showed SHH, SMO, GLI1, Bcl-2 and Foxm1 proteins expression in U87 and T98G cells treated with indicated concentrations of aspirin for 24 h. GAPDH was used as an internal control. 

26918349 23741443 28446712
Growth inhibition assay
Cell proliferation ; 

PubMed: 28446712     

U87 and T98G cells were treated with indicated concentrations of aspirin and harvested after 24, 48 and 72 h. Cell proliferation was analyzed by CCK-8 assay. 

Cell viability; 

PubMed: 30221683     

The CCK-8 assay results are presented as bar graphs for the antiproliferative effects of aspirin on RA-FLS. (A-C) RA-FLS were treated with (0, DMSO, 1, 2, 5 and 10 mM) aspirin for 12, 24 and 48 h. At each time interval, cell viability was determined by CCK-8 analysis. Data are presented as the means ± SD (error bars) from three independent experiments. Aspirin inhibited the growth of RA-FLS in a dose-dependent manner. (D) Cell proliferation decreased at 12, 24 and 48 h. Data are presented as the means ± SD (error bars) from three independent experiments. *P<0.05, **P<0.01, ***P<0.001 vs. DMSO group. CCK-8: Cell-Counting Kit-8; RA-FLS, rheumatoid arthritis-fibroblast-like synoviocytes; DMSO, dimethyl sulfoxide; SD, standard deviation.

28446712 30221683


Solubility (25°C)

In vitro DMSO 36 mg/mL (199.82 mM)
Ethanol 36 mg/mL (199.82 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 180.16


CAS No. 50-78-2
Storage powder
in solvent
Synonyms Acetylsalicylic acid
Smiles CC(=O)OC1=C(C=CC=C1)C(O)=O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)
% DMSO % % Tween 80 % ddH2O

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04356326 Not yet recruiting Drug: Aspirin 150 mg|Drug: Placebo Chronic Hypertension Complicating Pregnancy|Pre-Eclampsia|Intrauterine Growth Restriction|Aspirin|Perinatal Death|Placental Abruption Centre Hospitalier Intercommunal Creteil January 2021 Phase 3
NCT04295850 Not yet recruiting Drug: Aspirin 81 mg Preeclampsia Thomas Jefferson University April 1 2020 --
NCT04308551 Not yet recruiting Drug: Indobufen|Drug: Aspirin Stable Coronary Heart Disease Henan Institute of Cardiovascular Epidemiology March 16 2020 Not Applicable
NCT04325373 Recruiting -- Abdominal Aortic Aneurysm University Hospital Bordeaux March 6 2020 --
NCT04040465 Not yet recruiting Drug: Aspirin Aspirin Sensitivity University of Utah|University of Colorado Denver February 1 2020 Early Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

COX Signaling Pathway Map

Related COX Products

Tags: buy Aspirin | Aspirin supplier | purchase Aspirin | Aspirin cost | Aspirin manufacturer | order Aspirin | Aspirin distributor
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID