Aspirin

Catalog No.S3017 Synonyms: Acetylsalicylic acid

Aspirin Chemical Structure

Molecular Weight(MW): 180.16

Aspirin is a salicylate, and irreversible COX1 and COX2 inhibitor, used as an analgesic to relieve minor aches and pains, as an antipyretic to reduce fever, and as an anti-inflammatory medication.

Size Price Stock Quantity  
In DMSO USD 130 In stock
USD 97 In stock
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Purity & Quality Control

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Biological Activity

Description Aspirin is a salicylate, and irreversible COX1 and COX2 inhibitor, used as an analgesic to relieve minor aches and pains, as an antipyretic to reduce fever, and as an anti-inflammatory medication.
Targets
COX2 [1] COX1 [1]
In vitro

Aspirin inhibits the activation of NF-kappa B, thus prevents the degradation of the NF-kappa B inhibitor, I kappa B, and therefore NF-kappa B is retained in the cytosol. Aspirin also inhibits NF-kappa B-dependent transcription from the Ig kappa enhancer and the human immunodeficiency virus (HIV) long terminal repeat (LTR) in transfected T cells. [1] Aspirin and salicylate are mediated in part by their specific inhibition of IKK-beta, thereby preventing activation by NF-kappaB of genes involved in the pathogenesis of the inflammatory response. [2] Aspirin is protective against neurotoxicity elicited by the excitatory amino acid glutamate in rat primary neuronal cultures and hippocampal slices. [3] Aspirin triggers transcellular biosynthesis of a previously unrecognized class of eicosanoidsduring coincubations of human umbilical vein endothelial cells (HUVEC) and neutrophils [polymorphonuclear leukocytes (PMN)]. Aspirin evokes a unique class of eicosanoids formed by acetylated PGHS-2 and 5-lipoxygenase interactions. [4] Aspirin treatment inhibits the phosphorylation of IRS-1 at Ser307 as well as the phosphorylation of JNK, c-Jun, and degradation of IkappaBalpha in 3T3-L1 and Hep G2 cells treated with tumor necrosis factor (TNF)-alpha. Aspirin treatment inhibits phosphorylation of Akt and the mammalian target of rapamycin (but not extracellular regulated kinase or PKCzeta) in response to TNF-alpha. Aspirin rescues insulin-induced glucose uptake in 3T3-L1 adipocytes pretreated with TNF-alpha. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human AGS cells NXnRU4UyTnWwY4Tpc44h[XO|YYm= Mo\BNVUuPjBizsztc4wwVA>? M1XZc|EzKGh? NFnQcJdKdmirYnn0bY9vKG:oIFXzZ4hmemmlaHnhJINwdGlvc4TpcZVt[XSnZDDJUE05KHC{b3T1Z5Rqd25iaX6gbJVu[W5iQVfTJINmdGy|IHH0JFE2KHSxIE[wJJVud2xxTDDh[pRmeiBzMjDodpMh[nliRVzJV2E> MVuyNFE2OzF6Mx?=
human PC3 cells NXfGTIxwT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXXk[IdpOzByIN88US=> M3vWR|Q5KGh? NYL6[ZNoT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hWEN|IHPlcIx{KGG2IEOwNEB2VSCjZoTldkA1QCCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZk> M3;MZ|IzPDl2NkG3
human HCT116 cells NE\jfIZHfW6ldHnvckBie3OjeR?= NFrIUlQyKG2P M2rBOVYhcA>? NFXwOYxKdmirYnn0bY9vKG:oIGTOSk1idHCqYT3pcoR2[2WmIF7GMYtieHCjQjDhZ5RqfmG2aX;uJIlvKGi3bXHuJGhEXDFzNjDj[YxteyCjdDCxJI1OKGGodHXyJFYhcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigQ>? MYCyNlE2PDh|NB?=
human THP1 cells MXfGeY5kfGmxbjDhd5NigQ>? NF\K[2cyODBizszN MVSzNEBucW6| M3\wVmlzemW4ZYLzbYJt\SCrbnjpZol1cW:wIH;mJGNQYC1zIHnuJIh2dWGwIGTIVFEh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDhdoFkcGmmb37pZ{Bi[2mmLXnu[JVk\WRiVGjCNkBnd3KvYYTpc44h[XRiMUCwJJVOKGmwY4XiZZRm\CCob4KgN|AhdWmwczDmc4xtd3enZDDifUBkd22yb4Xu[EB4[XOqb4X0JI1m[XO3cnXkJFMxKG2rboOgdI9{fCCjcnHjbIlld26rYzDhZ4llKGOqYXzs[Y5o\SCkeTDyZYRqd2mvbYXuc4F{e2G7 MW[yN|Y2OTN3OR?=
human PANC1 cells M3TtTmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVSyOFhmOzByIN88US=> MlvqOFghcA>? NVzGSY9rT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hWEGQQ{GgZ4VtdHNiYYSgN|AxKHWPIHHmeIVzKDR6IHjyd{BjgSCjbHHtZZIh[my3ZTDhd5NigQ>? MU[yNlQ6PDZzNx?=
human SKBR3 cells MmXPS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4nz[FMxOCEQvF2= M1LBe|Q5KGh? MWPHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDTT2JTOyClZXzsd{BifCB|MECgeW0h[W[2ZYKgOFghcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5 MlTlNlI1QTR4MUe=
human MDA-MB-231 cells Mm[1SpVv[3Srb36gZZN{[Xl? NX22bmM{OTByIN88US=> NVvKe5VXOzBibXnudy=> MUHJdpJmfmW{c3nicIUhcW6qaXLpeIlwdiCxZjDDU3guOSCrbjDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yh[XKjY3jp[I9vcWNiYXPp[E1qdmS3Y3XkJHBITTJiZn;ycYF1cW:wIHH0JFExOCC3TTDpcoN2[mG2ZXSg[o9zKDNyIH3pcpMh\m:ubH;3[YQh[nliY3;tdI92dmRid3HzbI92fCCvZXHzeZJm\CB|MDDtbY5{KHCxc4SgZZJi[2irZH;ubYMh[WOrZDDjbIFtdGWwZ3WgZpkhemGmaX;pcY12dm:jc4PhfS=> Moe3NlM3PTF|NUm=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
MCL-1; 

PubMed: 26918349     


Western blot analysis of the effects of aspirin on MCL-1 expression.

p-AKT / AKT / p-ERK / ERK ; 

PubMed: 26918349     


Western blot analysis of the effects of aspirin on Akt and ERK1/2 phosphorylation.

p-AMPKα / AMPKα / p-ACC / ACC; 

PubMed: 26918349     


Western blot analysis of the effects of aspirin on AMPK and ACC phosphorylation.

PubMed: 23741443     


Western blotting revealed that aspirin down-regulated COX1 and COX2 expression in HCC cell lines.

SHH / SMO / GLI1 / Bcl-2 / Foxm1 ; 

PubMed: 28446712     


Western blot analysis showed SHH, SMO, GLI1, Bcl-2 and Foxm1 proteins expression in U87 and T98G cells treated with indicated concentrations of aspirin for 24 h. GAPDH was used as an internal control. 

26918349 23741443 28446712
Growth inhibition assay
Cell proliferation ; 

PubMed: 28446712     


U87 and T98G cells were treated with indicated concentrations of aspirin and harvested after 24, 48 and 72 h. Cell proliferation was analyzed by CCK-8 assay. 

Cell viability; 

PubMed: 30221683     


The CCK-8 assay results are presented as bar graphs for the antiproliferative effects of aspirin on RA-FLS. (A-C) RA-FLS were treated with (0, DMSO, 1, 2, 5 and 10 mM) aspirin for 12, 24 and 48 h. At each time interval, cell viability was determined by CCK-8 analysis. Data are presented as the means ± SD (error bars) from three independent experiments. Aspirin inhibited the growth of RA-FLS in a dose-dependent manner. (D) Cell proliferation decreased at 12, 24 and 48 h. Data are presented as the means ± SD (error bars) from three independent experiments. *P<0.05, **P<0.01, ***P<0.001 vs. DMSO group. CCK-8: Cell-Counting Kit-8; RA-FLS, rheumatoid arthritis-fibroblast-like synoviocytes; DMSO, dimethyl sulfoxide; SD, standard deviation.

28446712 30221683

Protocol

Solubility (25°C)

In vitro DMSO 36 mg/mL (199.82 mM)
Ethanol 36 mg/mL (199.82 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+PBS
For best results, use promptly after mixing.
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 180.16
Formula

C9H8O4

CAS No. 50-78-2
Storage powder
in solvent
Synonyms Acetylsalicylic acid

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04070573 Not yet recruiting Drug: acetylsalicylic acid Preeclampsia Weill Medical College of Cornell University October 2019 Phase 3
NCT04097912 Not yet recruiting Drug: Acetylsalicylic Acid (Aspirin BAYE4465) Myocardial Infarction|Stroke (Including Ischaemic Stroke and Transient Ischaemic Attack)|Unstable Angina|Angina|Ischaemic Heart Disease Bayer September 30 2019 --
NCT03188705 Not yet recruiting Drug: Clopidogrel|Drug: Aspirin Heart Diseases|Coronary Disease|Coronary Artery Disease|Cardiovascular Diseases|Myocardial Ischemia|Artery Occlusion|Aspirin Sensitivity|Clopidogrel Poor Metabolism of|Platelet Dysfunction|Platelet Thrombus University of Maryland Baltimore September 1 2019 Phase 4
NCT04040465 Not yet recruiting Drug: Aspirin Aspirin Sensitivity University of Utah|University of Colorado Denver August 1 2019 Early Phase 1
NCT04081831 Active not recruiting Drug: Acetylsalicylic Acid (Aspirin BAYE4465) Gastrointestinal Cancer Bayer July 31 2019 --

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COX Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID