Niflumic acid

Catalog No.S3018

For research use only.

Niflumic acid is an inhibitor of cyclooxygenase-2 used for joint and muscular pain.

Niflumic acid Chemical Structure

CAS No. 4394-00-7

Selleck's Niflumic acid has been cited by 1 Publication

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Biological Activity

Description Niflumic acid is an inhibitor of cyclooxygenase-2 used for joint and muscular pain.
Targets
COX-2 [1] GABA receptor [1]
In vitro

Niflumic acid inhibits Ca2+-activated Cl- channels with inhibition constant of 17 mM. Niflumic acid also inhibits ICl(Ca) elicited by bath application of Ca2+ to oocytes permeabilized using the Ca2+ ionophore A23187, demonstrating that the inhibition of ICl(Ca) is due to a direct interaction with the Cl- channel, rather than by interference with Ca2+ entry through voltage-dependent Ca2+ channels. [1] Niflumic acid blocks Ca2+-activated non-selective cation channels in inside-out patches from the basolateral membrane of rat exocrine pancreatic cells with IC50 of 50 μM. [2] Niflumic acid dose-dependently and reversibly activates large conductance calcium-activated K+ (KCa) channels. [3] Niflumic acid produces a concentration-dependent inhibition of spontaneous transient inward current (STIC, calcium-activated chloride current) amplitude. Niflumic acid inhibits noradrenaline- and caffeine-evoked IO(Ca) with an ICM50 of 6.6 μM, i.e.is less potent against evoked currents compared to spontaneous currents. Niflumic acid voltage-dependently inhibits spontaneous transient inward current (STIC) amplitude with IC50 of 2.3 μM and 1.1 μM at -50 and +50 mV respectively. [4] Niflumic acid inhibits not only IL-13-induced goblet cell hyperplasia but also airway hyperresponsiveness and eosinophilic infiltration. Niflumic suppresses the eotaxin levels in bronchoalveolar lavage fluids and overexpression of the MUC5AC gene, a marker of goblet cell hyperplasia, in the lung after IL-13 instillation. Niflumic acid suppresses JAK2 activation, STAT6 activation, and eotaxin expression in epithelial cells. [5]

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 56 mg/mL
(198.42 mM)
Water Insoluble
Ethanol Insoluble

Chemical Information

Molecular Weight 282.22
Formula

C13H9F3N2O2

CAS No. 4394-00-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC(=CC(=C1)NC2=C(C=CC=N2)C(=O)O)C(F)(F)F

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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